CN101263781A - Broccoli male sterility line transfer and hybrid preparation method - Google Patents
Broccoli male sterility line transfer and hybrid preparation method Download PDFInfo
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- CN101263781A CN101263781A CNA200710086575XA CN200710086575A CN101263781A CN 101263781 A CN101263781 A CN 101263781A CN A200710086575X A CNA200710086575X A CN A200710086575XA CN 200710086575 A CN200710086575 A CN 200710086575A CN 101263781 A CN101263781 A CN 101263781A
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Abstract
The invention discloses a transfer and hybrid compound method for the male sterile lines of broccoli, which is characterized in that, broccoli cytoplasmic male sterile heterozygote 725 is used as the female parent; broccoli inbred line 891 is used as the recurrent parent; after hybridization, all sterile plants with the pollen of flowers having no vigor are chosen to perform backcrossing; after 6 to 8 generations of transfer, broccoli cytoplasmic male sterile line green crown 1 is developed; all recurrent parents are the maintainer lines of corresponding cytoplasmic sterile lines 891; the hybrid compound method of the male sterile line of broccoli is that, cytoplasmic male sterile line green crown 1 is used as the female parent, and excellent early, middle and late inbred lines of broccoli are used as the male parent; the female parent and the male parent are respectively timely sown according to the ratio of 2 to 3:1; then first filial generation hybrid is prepared. The transfer and hybrid compound method for the male sterile line of broccoli has the advantages of low cost, high purity of the finished product and easy provenances.
Description
Technical field
The present invention relates to the vegetables cross breeding method, a kind of specifically seed selection of broccoli cytoplasm male sterility line and the method for hybrid seeding thereof.
Background technology
Broccoli, being subordinate to that Cruciferae (Cruciferae) rape belongs in the brassica specie with green or purple bouquet is a mutation of product, one, the biennial herb plant.Formal name used at school: Brassicaoleracea L.var.italica Planch.Chromosome number Zn=2x=18.Be to be developed by wild cabbage, evolution center is littoral area, east, Mediterranean.The eighties, China begins to have introduced some external broccoli kinds; The nineties, the broccoli cultivated area increases fast, but domestic broccoli breeding lags far behind other kinds of Cruciferae.The breeding of China at present is mainly based on the traditional breeding way of purification and rejuvenation, systematic breeding.Units such as vegetable or flower institute of the Chinese Academy of Agricultural Sciences, Shanghai City academy of agricultural sciences horticulture institute, Zhejiang Sky Good Seeds Co., Ltd. have carried out the seed selection work of broccoli kind in succession.The kind 90% above dependence on import of using in the production.Demand cultivating the improved seeds that independent intellectual property right is arranged urgently.Broccoli has tangible hybrid vigour, Hybrid adopts self incompatible line more, but self incompatible line breeding parent takes a lot of work, cost is higher, and inbreeding of more generation is prone to the vitality degeneration, the seed hybrid rate of first-filial generation is stable inadequately, and purity does not reach 100%.So be necessary to improve the breeding system of broccoli heterosis utilization, preferentially carry out the seed selection work of broccoli male sterile line.Seed selection also utilizes male sterile line production generation seed, can simplify production of hybrid seeds formality, saves cost, and can guarantee half-blooded seed purity.
Summary of the invention
A kind of breeding cost is low, purity is high and the transformation and the hybrid preparation method of the easy broccoli male sterile line of provenance in order to provide for purpose of the present invention.
The technical scheme that realizes the foregoing invention purpose is as follows:
The transfer method of broccoli male sterile line, with broccoli cytoplasmic male sterilty heterozygote 725 is female parent, with broccoli inbred line 891 is recurrent parent, the hybridization back selects the unvital sterile strain of all flower pollen to carry out backcross transformation, cultivates into the green hat 1 of broccoli cytoplasm male sterility line through 6-8 for transformation; Described recurrent parent is the maintenance line 891 of corresponding cytoplasmic sterile line.
The green hat 1 of male sterile line is sowed to produce male sterile line with 2～3: 1 ratio simultaneously with maintenance line 891.
The hybrid preparation method of broccoli male sterile line, the green hat 1 morning, noon and afternoon selfing maternal and that broccoli is good of cytoplasm male sterility line is a male parent, respectively with 2～3: 1 ratio is begun sowing in good time, and produces the first-filial generation hybrid.
The good inbred line male parent of described broccoli is a code name precocity 712,724, in ripe 716,718, late-maturing 720.
In the new broccoli cytoplasm male sterility line method of seed selection of the present invention, in the backcross transformation process, the standard of perfection of sterile strain is: all flower stamen WUHUAFEN of complete stool or through aceto-camine dyeing identify only have that minority is unvital, the malformed flower powder.
The transformation of broccoli male sterile line provided by the invention and hybrid preparation method are compared with seeding technique with existing conventional breeding has following advantage and good effect:
The inventive method practical, the green hat 1 of the broccoli cytoplasm male sterility line that utilizes the inventive method to breed, the new broccoli cytoplasmic sterile line of breeding is not subjected to the heredity stably of illumination or Temperature Influence, for the heterosis utilization of broccoli has been started new approach.Utilize the numerous system first cross of broccoli cytoplasm male sterility line seed, its production cost be selfing not parent line about 80%, the hybrid rate of hybrid seed can reach 100%, the purity that does not need to carry out crossbreed is identified, thereby save time, help the sale of hybrid seed, save cost owing to utilize the numerous system hybrid seed of broccoli cytoplasm male sterility line, can reduce hybrid new breed seed price, thereby help the rapid popularization of new varieties, can make new varieties give play to the huge social benefit sooner.
Description of drawings
Fig. 1 is a transfer method flow chart of the present invention
Fig. 2 is a hybrid preparation method flow chart of the present invention
Embodiment
The invention will be further described below in conjunction with the drawings and specific embodiments.
As shown in Figure 1, the transformation of broccoli male sterile line of the present invention and hybrid preparation method, its detailed content is as follows:
1. seed selection broccoli cytoplasm male sterility line
With can genetic stability and be not subjected to broccoli male sterile line 725 (the about 58cm of plant height of environmental influences such as illumination, temperature, degree of development 70cm, bouquet diameter 16.5cm, the heavy 500g of single ball, be cytoplasm male sterility line, stigma development is normal, the male flower WUHUAFEN, be 100% infertile) be sterile source, and as maternal; (late-maturing cold-resistant, growth potential is strong, the about 60cm of plant height, degree of development 85cm to be 891 with inbreeding of more generation disease-resistant, high yield, bouquet diameter 15.5cm, the heavy 510g of single ball, disease resistance is strong, pollen is many) be the samsara male parent, hybridize, obtain F1 and hybridize with recurrent parent again, obtain BC1 generation; In BC1 generation, select all flower WUHUAFEN of complete stool or aceto-camine dyeing identify the sterile strain that minority abortion, unvital malformed flower powder are only arranged, carry out backcross transformation with the samsara male parent, through 6 generations evaluation select, breed sterile rate and sterile degree and can both reach 100%, and the green hat 1 of broccoli cytoplasm male sterility line (plant height 63cm, degree of development 93cm, the long 23cm of petiole of energy genetic stability, bouquet diameter 16cm, the heavy 600g of single ball).Its samsara male parent is respectively the maintenance line of green hat 1.
2. utilize the green hat 1 of broccoli cytoplasm male sterility line, the method for numerous production of hybrid seeds
(1) numerous system method of green hat 1 of broccoli cytoplasm male sterility line and corresponding maintenance line 891 thereof.
The selection fertility level is higher, the area that is fit to numerous kind of broccoli, adopt the breed place of plastic tunnel (isolating) as green hat 1 with other crop in cruciferae, green hat 1 of male sterile line and maintenance line 891 are sowed simultaneously with 2: 1 ratio, male sterile line and maintenance line interlacing field planting, put honeybee (produced in small quantities can be carried out manually auxiliary) pollination flowering stage, simultaneously the maintenance line plant is carried out selfing and reserve seed for planting.The Sheng phase topdresses as a result, in conjunction with the field extermination of disease and insect pest, and the threshing of in time gathering after the fruit pod maturation.The seed of gathering on the male sterile line plant is numerous kind of required male-sterile seed, and the seed of gathering on the maintenance line plant is the maintenance line seed.
(2) utilize the method for the green hat 1 numerous system first cross seed of broccoli cytoplasm male sterility line.
The selection fertility is higher, the area that is suitable for numerous kind of broccoli, adopt plastic tunnel or open country (more than the isolated area 1000m) as half-blooded numerous system place, each plants green hat 1 of male sterile line and good inbred line 712 grades with 2: 1 ratio, each parent's sowing time begins sowing in good time according to the flower synchronization principle, puts honeybee pollination flowering stage.Impose P, K and boron fertilizer in pod bearing period,, pull out the inbred line male parent after hybridization pollination finishes in conjunction with the extermination of disease and insect pest, the fruit pod maturation threshing of in time gathering on the male sterile line plant, the seed of being gathered in the crops is the first cross seed of required numerous system.
The green hat 1 of cytoplasm male sterility line with new seed selection is a female parent, with the broccoli inbreeding of more generation of the ripe property of difference is 712,724,718,716,720 to be male parent, the preparation combination, all show as stronger hybrid vigour as a result after the assembly, plant height on average increases 10cm, carries out more than the Du Keda 90cm with the combination of slow ripe inbred line preparation.Show that these advantageous combination are obvious, the combination of preparation is comparatively successful.The preparation commodity of its medium green hat 1x712 early maturing variety is good, and comprehensive proterties is approaching or surpass present main breed, and growth is fast, the bouquet bottle green, and bud is thin, and commodity is good, can be used as ealy maturing culture.Green hat 1x718 middle seasonal strain, disease resistance is strong, and comprehensive proterties is good, spends carefully, and ball is emerald green, and commodity is good.
Following table 1 is green hat 1 seed selection process of broccoli cytoplasm male sterility line and fruit properties table
Table 1
| Sequence number | Parent and transformation are from generation to generation | Plant height (cm) | Degree of development (cm) | Leaf long (cm) | Leaf wide (cm) | Petiole long (cm) | Bouquet diameter (cm) | Single ball heavy (g) | Receipts phase beginning (my god) |
| 1 | 725 female parents | 58.2 | 69.8 | 33.5 | 18.5 | 23.4 | 16.5 | 500 | 72 |
| 2 | 891 male parents | 60.5 | 85.2 | 32.5 | 19.5 | 22 | 15.5 | 510 | 110 |
| 3 | 725x891F1 | 62.5 | 92.5 | 38.5 | 20.3 | 23 | 15.5 | 600 | 98 |
Claims (4)
1. the transfer method of broccoli male sterile line, with broccoli cytoplasmic male sterilty heterozygote 725 is female parent, with broccoli inbred line 891 is recurrent parent, the hybridization back selects the unvital sterile strain of all flower pollen to carry out backcross transformation, cultivates into the green hat 1 of broccoli cytoplasm male sterility line through 6-8 for transformation; Described recurrent parent is the maintenance line 891 of corresponding cytoplasmic sterile line.
2. the transfer method of broccoli male sterile line according to claim 1 is characterized by the green hat 1 of male sterile line and sows simultaneously to produce male sterile line with 2～3: 1 ratio with maintenance line 891.
3. the hybrid preparation method of broccoli male sterile line, the green hat of cytoplasm male sterility line 1 maternal with the good morning, noon and afternoon selfing of broccoli be male parent, respectively with 2～3: 1 ratio is begun sowing in good time, and produces the first-filial generation hybrid.
4. the hybrid preparation method of broccoli male sterile line according to claim 3, it is characterized by the good inbred line male parent of described broccoli is code name precocity 712,724, in ripe 716,718, late-maturing 720.
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| CNA200710086575XA CN101263781A (en) | 2007-03-15 | 2007-03-15 | Broccoli male sterility line transfer and hybrid preparation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835306A (en) * | 2012-09-26 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for early-maturing hybrid broccoli in additive selection |
| CN107455255A (en) * | 2017-09-08 | 2017-12-12 | 江苏丘陵地区镇江农业科学研究所 | A kind of selection of broccoli |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835306A (en) * | 2012-09-26 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for early-maturing hybrid broccoli in additive selection |
| CN102835306B (en) * | 2012-09-26 | 2014-03-12 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for early-maturing hybrid broccoli in additive selection |
| CN107455255A (en) * | 2017-09-08 | 2017-12-12 | 江苏丘陵地区镇江农业科学研究所 | A kind of selection of broccoli |
| CN107455255B (en) * | 2017-09-08 | 2019-08-02 | 江苏丘陵地区镇江农业科学研究所 | A kind of selection of broccoli |
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Open date: 20080917 |