CN1910999A - Method for reproducing and producing hybrid seeds of tomato with crack-resistance and of pink color fruits - Google Patents
Method for reproducing and producing hybrid seeds of tomato with crack-resistance and of pink color fruits Download PDFInfo
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- CN1910999A CN1910999A CN 200610015436 CN200610015436A CN1910999A CN 1910999 A CN1910999 A CN 1910999A CN 200610015436 CN200610015436 CN 200610015436 CN 200610015436 A CN200610015436 A CN 200610015436A CN 1910999 A CN1910999 A CN 1910999A
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Abstract
A method for culturing the anticracking hybridized tomato 'Jinza 206' with farinaceous fruit features the artificial hybridization between selfing line Ta-BD and selfing line Roma VFP.
Description
Technical field
The invention belongs to agricultural breeding, particularly relate to a kind of crossbreeding method of cracking resistance Steamed dumpling with pork seeds of hybridized tomato.
Background technology
Since nineteen ninety-five, the consumption figure of global tomato and converted products thereof keeps the trend of steady growth always.Tomato also is one of important bulk vegetable crop of China, because tomato has salt resistance ability preferably, so particularly powerful in China's southeastern coastal areas growth momentum, protection ground tomato has become the major product of foreign exchange earning, increasing peasant income.Up-to-date result of study shows, lycopene in the tamato fruit has special physiological function, the intake that increases lycopene can reduce the incidence of disease of multiple chronic disease and cancer effectively, and the incidence of the concentration of lycopene and gastrointestinal cancer, prostate cancer, cancer of pancreas, cervical carcinoma etc. is negative correlation in the human serum.Therefore, the demand of tomato is also constantly strengthening.
Because be subjected to the influence of factors such as weather, geographical conditions, the Steamed dumpling with pork tomato is the main breed of China North China, and majority is all produced on the ground in protection.The pericarp water white transparency of this veriety, hardness is lower, and its maturing stage is easy to produce slight crack around the fruit navel, and the storage capability of fruit and the ability of opposing infected by microbes are descended significantly.According to estimates, the economic loss that dehiscent fruit caused accounts for 20% of vegetable grower's annual value of production.China North China presses for the Steamed dumpling with pork tomato new varieties of cracking resistance fruit, storage tolerance.
Summary of the invention
Problem at dehiscent fruit, the inventor has carried out a large amount of work, it is low finally to study, filter out a kind of dehiscent fruit rate, and the good Steamed dumpling with pork tomato new varieties of comprehensive agronomy proterties such as yielding ability, disease resistance, and the hardness of fruit, softening rate, pericarp elasticity etc. are greatly improved.
The crossbreeding method that the purpose of this invention is to provide the seeds of hybridized tomato that a kind of dehiscent fruit rate is low, comprehensive agronomy proterties such as yielding ability good, disease resistance are good.
The present invention seeks to realize by the following technical solutions:
1) being that the big powder in landrace Baoding of female parent, China is a paternal hybrid with the tomato variety RomaVF that introduces from the U.S., is that male parent is backcrossed once with the big powder in Baoding again; Select the low Steamed dumpling with pork individual plant of dehiscent fruit rate from separating the offspring, successive selection obtains 23 parts of inbred lines after 5 generations.Fruit structure, hardness and softening rate etc. to these inbred lines are identified, therefrom filter out the minimum inbred line RomaVFP of dehiscent fruit rate.
With from the Stone (landrace that U.S. tomato genetic research center TGRC provides, the unified LA1506 that is numbered) the anti-storage of the inbred line cracking resistance fruit tomato strain Ta14 that screens in is paternal hybrid for commercial crossbreed precious big 903 maternal, China, from the Steamed dumpling with pork individual plant of segregative generation selection from the type that binds, successive selection obtains 13 parts of type inbred lines that bind certainly that the dehiscent fruit rate is low after 5 generations.To their yielding ability with after the dehiscent fruit rate tests, filter out from binding type Steamed dumpling with pork inbred line Ta-BD.
2) be female parent with the type tomato inbred line Ta-BD that binds certainly, be male parent or be female parent that with indeterminate growth type tomato inbred line RomaVFP Ta-BD is that male parent is carried out artificial hybridization, breed F1 for crossbreed Tianjin assorted 206 with RomaVFP.
Inbred line Ta-BD or inbred line RomaVFP adopt the mode of growing seedlings early spring or directly sowing.Grow seedlings early spring and generally in greenhouse by solar heat or plastic tunnel, carry out, when 4 leaves, 1 heart, ground temperature are stablized above 10 ℃, carry out transplant planting when seedling is long.Planting density in the greenhouse by solar heat is 3500-4000 strain/mu, and the plantation density of outdoor cropping is 5000 strains.
Crossbreed Tianjin assorted 206 is that the maternal inbred line Ta-BD of being, male parent are that inbred line RomaVFP or male parent are the single cross combination that inbred line Ta-BD, female parent form for inbred line RomaVFP hybridization.The crossbreed that two kinds of hybridization modes obtain does not have difference on Main Agronomic Characters such as yielding ability, disease resistance, quality.The row of seed farm male parent and female parent is than being 2: 10～12.Under the situation of the sowing same period, maternal Ta-BD was generally bloomed ahead of time than male parent RomaVFP in 5 days, can guarantee flower synchronization.Begun just at maternal Ta-BD calyx that bifurcated opens, when flower pesticide begins by green flavescence, artificial removal's flower pesticide is castrated in back 1-3 days, gives maternal column cap pollination with paternal pollen.If, Ta-BD maternal with the RomaVFP work makes male parent, then should divide two phases sowing Ta-BD, to prolong the flowering time of male parent, guarantee enough pollen.
The present invention has the following advantages and characteristics:
1, the many fruits of fruit are even, yielding ability is outstanding: tomato Tianjin assorted 206 performance in the variety comparative test of multiple years is very outstanding, and 3 output gathered 6510 kilograms of average yield per mus in 2005, comparison according in assorted 9 volume increase 39.4%; 3 output gathered 5800 kilograms of average yield per mus in 2006, assorted 9 volume increase, 21.3% (table 1) during comparison is shone.
2, the dehiscent fruit rate is low, and the hardness of fruit, softening rate, pericarp elasticity etc. obtain improvement, anti-storage, commodity rate height.The dehiscent fruit rate is less than 20%, and hardness is 5.4 * 10
5Pa, softening rate are 5.9 * 10
3Pa/ days.
3, low temperature tolerance ability strengthens, suitable cultivation in early spring.
4, fruit-setting rate height, taste flavor is good, and fruit expands soon.
5, amphilepsis difference is big, with dna fingerprinting they and filial generation thereof is made a distinction (table 2) easily.
Description of drawings
The crossbreeding programme diagram of Fig. 1 inbred line parent Ta-BD of the present invention and RomaVFP;
The crossbreeding programme diagram in Fig. 2 crossbreed of the present invention Tianjin assorted 206.
Embodiment
Below by specific embodiment, be described in further detail the present invention.
Embodiment 1
The landrace that Stone provides for U.S. tomato genetic research center TGRC, the unified LA1506 that is numbered.
Referring to Fig. 1, Fig. 2.The selection gutless good farmland of tomato of crossing last year is as inbred line seminal propagation field.For fear of the biology hybrid that insect pollination causes, 5 meters wide rapes of plantation or the contour stalk crop of sunflower between different inbred lines carry out barrier and isolate.
1, the cultivation of parent RomaVFP:
With the tomato variety RomaVF that introduces from the U.S. is that the big powder in landrace Baoding of female parent, China is a paternal hybrid, is that male parent is backcrossed once with the big powder in Baoding again; Select the low Steamed dumpling with pork individual plant of dehiscent fruit rate from separating the offspring, successive selection obtains 23 parts of inbred lines after 5 generations.Fruit structure, hardness and softening rate etc. to these inbred lines are identified, therefrom filter out the minimum inbred line RomaVFP of dehiscent fruit rate.
Inbred line RomaVFP is an indeterminate growth type tomato, and the fruit Long Circle is easy to make a distinction with other kinds.After first bunch of fruit enters colour-change period, check plant type, fruit shape, fruit look and disease resistance one by one in the breeding field, pull out assorted strain and diseased plant, gather in the crops full ripe fruit then by stages, obtain high-quality tomato seeds with fermentation method.
The feature of RomaVFP: high bar, upright, split carve leaf, disease resistance is good, the big fruit of fruit is even, yielding ability is good, the stem stalk is sturdy, the pericarp water white transparency, epidermal cell and corneocyte thicker (about 45 μ m), anti-crack ability is strong, pink fruit, single fruit weight 150g, fruit type inbred line in the genus.Flower pesticide is big, pollen amount is sufficient, artificially collects flower pesticide and pollen easily.About 120 days breeding times, single raceme, indeterminate growth type.
2, the cultivation of parent Ta-BD:
Self progeny by landrace Stone that the U.S. is introduced carries out system and selects, and filters out the anti-storage tomato strain Ta14 of cracking resistance fruit.With Ta14 is that the commercial crossbreed precious big 903 of maternal China is a paternal hybrid, selects Steamed dumpling with pork individual plant from the type that binds from segregative generation, and successive selection obtains 13 parts of type inbred lines that bind certainly that the dehiscent fruit rate is low after 5 generations.To their yielding ability with after the dehiscent fruit rate tests, filter out from binding type Steamed dumpling with pork inbred line Ta-BD.
Inbred line Ta-BD is a determinate growth type tomato, and 5-6 bunch of inflorescence binds when occurring automatically, and internode is short between the titbit, and whole plant type is shorter, and flowering time is concentrated, the fruit-setting rate height.When first bunch of fruit enters colour-change period, check plant type and fruit look in the breeding field one by one, pull out indeterminate growth type and short especially determinate growth type plant, gather in the crops full ripe fruit then by stages, ferment after mashing and rinsing, obtain high-quality inbred line seed.These inbred line seeds are carried out airing and clean, carry out the check of indexs such as germination rate, water content, purity, carry out packing then, labelled, be placed on shady and cool place and preserve.
The feature of Ta-BD: of short stem, upright, split carve leaf, low temperature resistant, disease resistance good, fruit is big, yielding ability is good, the flat garden of fruit shape, smooth surface, the pericarp water white transparency, pink fruit, single fruit weight is generally 180g, belongs to the large fruit inbred line.Plant type compactness, titbit characteristics in great numbers, suitable dense planting, the fruit-setting rate height is easy to the artificial emasculation pollination.About 120 days breeding times, single raceme, determinate growth type.
3, the cultivation in cracking resistance Steamed dumpling with pork seeds of hybridized tomato Tianjin assorted 206:
With inbred line RomaVFP is that female parent, inbred line Ta-BD are male parent or are that female parent, inbred line RomaVFP are male parent with inbred line Ta-BD, breeds F1 for crossbreed Tianjin assorted 206.
Seed farm requirement fertility is higher, irrigation and drainage convenient, guarantees the higher natural fruit-setting rate and the normal development of fruit.In order to concentrate the artificial hybridization seed of gathering; be that female parent, indeterminate growth type inbred line RomaVFP are male parent generally with determinate growth type inbred line Ta-BD; according to 2 row male parents: the maternal ratios of 10 row are planted male parent inbred line and maternal inbred line successively, around the piece of field all plantation male parent inbred lines as guard row and pollen deposit colony.When maternal calyx begins to open, the male flower color is during by green flavescence, manually extract male flower, and 2 calyx are fractureed as artificial emasculation pollination's mark.Collect the male flower of foresythia simultaneously from the male parent inbred line, be placed on indoor drying, the male flower that dries is collected in the dixie cup smashs to pieces, visible a large amount of yellow powder is pollen grain at the cup end.Rubber tip with pencil is stained with pollen grain, carries out artificial pollination for 2-4 days the maternal inbred line in castration back.The florescence of seed farm Ta-BD should shift to an earlier date 1-2 days than RomaVFP.If the advance flowering period of RomaVFP can be carried out artificial emasculation to the RomaVFP of early flowering, with the pollen pollination of Ta-BD, the crossbreed of gained is just the same then.If seed farm all with RomaVFP do female parent, Ta-BD does male parent, then must carry out interval sowing to Ta-BD, otherwise the pollen in later stage can not get guaranteeing.
About 40 days, fruit begins maturation after the artificial pollination.Results lack the ripening fruits of 2 calyx by stages, mash after fermentation 2-3 days, dry behind the rinsing seed, clean classification then, test germination rate, purity etc.The artificial hybridization seed that reaches national secondary seed standard is carried out commercial packing, enter the seed market sale.Commodity Tianjin assorted 206 by name.
The feature in Tianjin assorted 206: cane is upright, splits the leaf at quarter, about 120 days breeding times, and single raceme, pericarp water white transparency, fruit pink colour.Eat the tomato new varieties raw for indeterminate growth type Steamed dumpling with pork, fruit-setting rate is moderate, spends for every bunch and generally can sit 6 fruits, and fruit expands soon, the output height.Fruit does not have green shoulder, and size evenly, and is really circular, and fruit shape index about 1.1, single fruit weight are generally the 200-250 gram, and single fruit weight can reach 300 grams when fertilizer and water condition was better; Hardness of fruit when ripe is 5.4 * 10
5Pa, softening rate are 5.9 * 10
3(hardness of fruit of contrast was 5.2 * 10 in Pa/ days
5Pa, softening rate are 20 * 10
3Pa/ days); Pol is higher, and taste flavor is good.Low temperature tolerance ability is strong, suitable cultivation in early spring.
The variety comparative test result of table 1 seeds of hybridized tomato TJ206 multiple years
| Time | The place | Area code | Harvest time | Output (kg/ mu) | In assorted 9 output | Volume increase % |
| 2005 | The Tianjin Xiqing District | TJ206 | 5.27-6.06 | 6,580 | 4,960 | 32.7 |
| Tianjin agricultural Demonstration Garden | TJ206 | 5.10-6.25 | 7,200 | 4,750 | 51.6 | |
| The Feng County, Jiangsu Province | TJ206 | 4.28-6.06 | 5,750 | 4,300 | 33.7 | |
| 3 average | - | 6,510 | 4,670 | 39.4 | ||
| 2006 | The Tianjin Xiqing District | TJ206 | 5.10-6.20 | 5,520 | 4,600 | 20.0 |
| Tianjin agricultural Demonstration Garden | TJ206 | 5.14-6.25 | 5,800 | 4,790 | 21.1 | |
| The Feng County, Jiangsu Province | TJ206 | 4.26-6.05 | 6,080 | 4,950 | 22.8 | |
| 3 average | - | 5,800 | 4,780 | 21.3 | ||
| - | ||||||
| 2 years 6 average | - | 6,155 | 4,725 | 30.3 |
Table 2 seeds of hybridized tomato Tianjin assorted 206 and parents' thereof dna fingerprinting
| The primer title | at | caa | ga | tat | 241 | 279 | 287 |
| Tianjin assorted 206 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
| RomaVFP | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
| Ta-BD | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
Claims (3)
1, a kind of crossbreeding method of cracking resistance Steamed dumpling with pork seeds of hybridized tomato is characterized in that:
1) is female parent with the type tomato inbred line Ta-BD that binds certainly, is male parent or is female parent that with indeterminate growth type tomato inbred line RomaVFP Ta-BD is that male parent is carried out artificial hybridization, improved crossing kind Tianjin assorted 206 with RomaVFP;
2) male parent and maternal field planting ratio are 2: 10～12 in assorted 206 seed farms in crossbreed Tianjin.
2, the crossbreeding method of cracking resistance Steamed dumpling with pork seeds of hybridized tomato according to claim 1, it is characterized in that: inbred line Ta-BD or inbred line RomaVFP grew seedlings in early spring, when growing to 4 leaves, 1 heart, seedling carries out transplant planting, planting density in the phytotron is 3500-4000 strain/mu, and the plantation density of outdoor cropping is 5000 strains.
3, the crossbreeding method of cracking resistance Steamed dumpling with pork seeds of hybridized tomato according to claim 1 is characterized in that: when maternal flower pesticide begins by green flavescence, and artificial removal's flower pesticide; Castrate in back 1-3 days, carry out artificial pollination with the pollen of male parent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610015436 CN1910999A (en) | 2006-08-25 | 2006-08-25 | Method for reproducing and producing hybrid seeds of tomato with crack-resistance and of pink color fruits |
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| CN 200610015436 CN1910999A (en) | 2006-08-25 | 2006-08-25 | Method for reproducing and producing hybrid seeds of tomato with crack-resistance and of pink color fruits |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835309A (en) * | 2012-10-11 | 2012-12-26 | 浙江省农业科学院 | Method for breeding inbred line of purple solanum lycopersicum |
| CN104885904A (en) * | 2014-09-28 | 2015-09-09 | 安徽国盛农业科技有限责任公司 | Tomato hybrid seed production marking method |
| CN112544439A (en) * | 2020-12-28 | 2021-03-26 | 江苏省农业科学院 | Exquisite tomato cross pollination method |
| CN114807192A (en) * | 2022-04-26 | 2022-07-29 | 南京农业大学 | BSR sequencing-based identification of tomato irregular fruit cracking key gene SlGH9-15 and application |
-
2006
- 2006-08-25 CN CN 200610015436 patent/CN1910999A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835309A (en) * | 2012-10-11 | 2012-12-26 | 浙江省农业科学院 | Method for breeding inbred line of purple solanum lycopersicum |
| CN102835309B (en) * | 2012-10-11 | 2014-02-26 | 浙江省农业科学院 | Method for breeding inbred line of purple solanum lycopersicum |
| CN104885904A (en) * | 2014-09-28 | 2015-09-09 | 安徽国盛农业科技有限责任公司 | Tomato hybrid seed production marking method |
| CN112544439A (en) * | 2020-12-28 | 2021-03-26 | 江苏省农业科学院 | Exquisite tomato cross pollination method |
| CN114807192A (en) * | 2022-04-26 | 2022-07-29 | 南京农业大学 | BSR sequencing-based identification of tomato irregular fruit cracking key gene SlGH9-15 and application |
| CN114807192B (en) * | 2022-04-26 | 2023-09-22 | 南京农业大学 | Identification of tomato irregular dehiscence key gene SlGH9-15 based on BSR sequencing and application |
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