CN102835309B - Method for breeding inbred line of purple solanum lycopersicum - Google Patents
Method for breeding inbred line of purple solanum lycopersicum Download PDFInfo
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- CN102835309B CN102835309B CN201210383516.XA CN201210383516A CN102835309B CN 102835309 B CN102835309 B CN 102835309B CN 201210383516 A CN201210383516 A CN 201210383516A CN 102835309 B CN102835309 B CN 102835309B
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Abstract
The invention discloses a method for breeding an inbred line of purple solanum lycopersicum, and belongs to the technical field of plant breeding. The method comprises the following steps of: 1) preparing a red F1-generation hybrid by using red and yellow solanum lycopersicum; 2) hybridizing the red F1-generation hybrid which serves as a male parent or a female parent and green solanum lycopersicum to obtain a three-way cross hybrid; 3) back-crossing the red three-way cross hybrid by using the green solanum lycopersicum as a male parent or a female parent to obtain a BC1 generation; 4) selecting a single green plant from a BC1-generation segregation population, and performing inbreeding to obtain a BC1F2-generation seed; 5) selecting the purple solanum lycopersicum from the BC1F2-generation seed for inbreeding seed reservation; and 6) performing inbreeding homozygosis of three to five generations to obtain the inbred line of the purple solanum lycopersicum. A novel breeding means is provided for germplasm resources of the purple solanum lycopersicum, can be used for breeding purple solanum lycopersicum hybrids with distinct advantages, and can be popularized and applied to breeding and production.
Description
Technical field
The present invention relates to field of plant variety breeding technology, relate in particular to a kind of method that tomato that utilizes three kinds of different fruit colors of red, yellow, and green is cultivated dissimilar purple Tomato Germplasm.
Background technology
Tomato (
solanum lycopersicum) be one of important in the world vegetable crop, approximately 398.9 ten thousand hectares of Annual planting areas, 10849.9 ten thousand tons of gross yields (FAO, 2010).Though tomato is short at China's cultivation history, become one of main vegetable species.Purple tomato is kind very unique in tomato, postgraduate Ka Er Jones of Ore. in 2000 finds purple tomato except the carotin and lycopene that contain common tomato and have by the research that this tomato nutrient is worth, also contain the unexistent anthocyan of other tomatoes, nutritive value is higher than common tomato.Purple tomato originates from the original jungle in America, the 60 to 70's of 20th century scientists has been collected wild purple Tomato Germplasm, wild purple tomato is at that time with toxic can not eating, and scientists is by having cultivated edible purple Tomato Germplasm with common cultivation kind crossbreeding and improvement method.Yet, between the purple Tomato Germplasm Resources of cultivating by this means, genotype is comparatively close, after hybridization, hybrid vigour is not obvious, therefore purple tomato variety mostly in the market is conventional, plants, and easily causes the adverse consequencess such as the loss of germ plasm resource and deterioration of variety.The research group that Britain John Ying Nisi center professor Kai Xi Martin in 2008 leads utilizes the characteristic that snapdragon anthocyanidin content is high, by two kinds of gene transfer of this Plants to common tomato, cultivate the tomato that a kind of outward appearance is purple, but at transgenic foods safety, fail to obtain to see clearly completely now, purple tomato not yet can get the nod and promote this transgenosis.Up to now, the breeding method of the purple tomato having good grounds only has above-mentioned two kinds, and innovates and the method for cultivating purple tomato yet there are no disclosed report by Tomato Germplasm Resources red, yellow and green three kinds of different colours.
Summary of the invention
The present invention seeks to, the problem of and edible safety single for the existing genotype of existing purple tomato, provides that a kind of cultivation period is short, economical character good, the method for the inbred line purple Tomato Germplasm Resources that isozygotys of different genotype.
The object of the invention is achieved by the following technical programs.
A selection for purple tomato inbred line, the method is carried out according to the following steps:
1) with red tomato, do male parent or maternal and tomato yellow hybridization, obtaining fruit color is red F1 generation crossbreed;
2), using F1 generation crossbreed as male parent or maternal and green tomato hybridization, obtain fruit color for redness and yellow triple hybrid;
3) usining green tomato as male parent or female parent, is that red triple hybrid backcrosses and obtains backcrossing BC1 generation to fruit color;
4) BC1 is respectively red, yellow and three green types of populations for being separated into fruit color, therefrom selects the good green tomato individual plant of Agronomic to carry out selfing and reserves seed for planting, and obtains BC1F2 for seed;
5) BC1F2 is respectively colony green and purple for being separated into fruit color, therefrom selects the good purple tomato individual plant of economical character to carry out selfing and reserves seed for planting;
6) above-mentioned selfed seed is carried out after 3-5 isozygotys for selfing again, therefrom select to obtain the purple tomato inbred line of color, economical character stable and consistent.
Described red tomato is the tomato variety of choosing any one kind of them from T07-026, Zhejiang assorted 203 and Zheza 204.
Described tomato yellow choosing is the tomato variety of choosing any one kind of them from T07-021 and CH03-056.
Described green tomato is CH09-004.
The invention has the beneficial effects as follows:
1, the present invention utilize existing germ plasm resource by hybridizing, backcross, the technological means such as selfing, separated selection carries out genetic recombination, created the new method that purple Tomato Germplasm Resources is cultivated in a kind of tomato material hybridization that utilizes redness, yellow and green three kinds of different colours, for the establishment of purple Tomato Germplasm Resources provides a kind of new breeding approach.
2, the purple tomato inbred line germ plasm resource that the present invention cultivates has the genotype different from existing purple kind, utilizes it as parent material, can cultivate into new purple seeds of hybridized tomato, more can fully demonstrate hybrid vigour;
3, the present invention can be the further investigation of Tomato Fruit Color genetic development, and new material base is provided.
Embodiment
By following examples, the present invention is described in further detail, but content of the present invention is not limited to this.
Explanation to the related material of following examples:
Red tomato material: T07-026 original number is H24, introduces from vegetables center, Asia (AVRDC); ' Zhejiang assorted 203 ' and ' Zheza 204 ' are by the autonomous red tomato first generation of hybrid kind being bred as of Zhejiang Academy of Agricultural Science Vegetable Research Institute, in 2007, obtained every year " the new variety of agricultural plant protection warrant book " that China Ministry of Agriculture ratifies, and announce on < < new variety of agricultural plant protection communique > >, wherein ' assorted 203 ' the kind power number in Zhejiang is CNA20040001.0, and ' Zheza 204 ' kind power number is CNA20040002.9;
Tomato yellow material: T07-021 original number is FLA456-4, introduces from vegetables center, Asia (AVRDC); " the yellow pearl cherry and tomato ”，Cong Chinese Academy of Agricultural Sciences introduces CH03-056 original name;
Green tomato material: CH09-004 original name " the red emerald in capital ", from agricultural and forest science institute vegetables center, Beijing, introduce.
The seed selection of embodiment 1:(purple tomato CL1-1, CL1-2 inbred line)
1) the 7-11 month in 2007: take red tomato T07-026 as maternal, the tomato yellow of take is hybridized as male parent for No. T07-021, obtains F1 generation material 2621, and fruit color is red;
2) in December, 2007-2008 year June: take green tomato CH09-004 as maternal, take and 2621 hybridize as male parent, obtain triple hybrid 09-004 * 2621, fruit color is redness and yellow;
3) the 7-11 month in 2008: the CH09-004 of take is maternal as samsara, and in 09-004 * 2621, red plant is male parent, hybridizes and obtains first backcross generation 09-004 * 2621BC1;
4) in December, 2008-2009 year June: 100 strains of plantation first backcross generation plant, fruit color is separated into redness, yellow and green three kinds, therefrom selects to grow, the good green tomato individual plant of Bearing performance carries out selfing and reserves seed for planting, and obtains BC1F2 for seed;
5) in July, 2009-2009 year November: 200 strains of plantation BC1F2 plant, fruit color is separated into green and purple, therefrom selects growth, the good purple tomato individual plant of Bearing performance, and selfing is reserved seed for planting;
6) in December, 2009-2011 year November: obtain stable purple tomato inbred line through selfing continuously 4 generations of isozygotying, therefrom select individual plant CL1-1, CL1-2 that proterties is good.
The seed selection of embodiment 2:(purple tomato CL2-1, CL2-2 inbred line)
1) in December, 2006-2007 year June: take tomato yellow CH03-056 as maternal, take red tomato Zhejiang assorted 203 to hybridize as male parent, the F1 generation material number obtaining is 5603, and fruit color is redness;
2) the 7-11 month in 2007: take green tomato CH09-004 as maternal, take and 5603 hybridize as male parent, obtain triple hybrid 09-004 * 5603, fruit color is redness and yellow;
3) in December, 2007-2008 year June: the red plant in 09-004 * 5603 of take, as maternal, be take CH09-004 as samsara male parent, hybridizes and obtains first backcross generation 09-004 * 5603BC1;
4) the 7-11 month in 2008: 100 strains of plantation first backcross generation 09-004 * 5603BC1 plant, fruit color is separated into redness, yellow and green three kinds, selects to grow, the good green tomato individual plant selfing of Bearing performance reserves seed for planting, and obtains BC1F2 for seed;
5) in December, 2008-2009 year June: 200 strains of plantation BC1F2 plant, fruit color is separated into green and purple, therefrom selects growth, the good purple tomato individual plant of Bearing performance, and selfing is reserved seed for planting;
6) in July, 2009-2011 year November: obtain stable purple tomato inbred line through 5 generations of selfing continuously, therefrom select individual plant CL2-1, CL2-2 that proterties is good.
The seed selection of embodiment 3:(purple tomato CL3-1, CL3-2 inbred line)
1) the 7-11 month in 2007: take red tomato Zheza 204 as maternal, the tomato yellow CH03-056 of take is hybridized as male parent, and obtaining F1 generation material number is 0456, and fruit color is red;
2) in December, 2007-2008 year June: take 0456 as maternal, the green tomato CH09-004 of take is hybridized as male parent, obtains triple hybrid 0456 * 09-004, and fruit color be redness and yellow;
3) the 7-11 month in 2008: the red plant in 0456 * 09-004 of take is maternal, and the CH09-004 of take is hybridized and obtained first backcross generation 0456 * 09-004BC1 as samsara male parent;
4) in December, 2008-2009 year June: plantation first backcross generation 0456 * 09-004BC1 plant 100 strains, fruit color is separated into redness, yellow and green three kinds, therefrom select growth, the good green tomato individual plant selfing of Bearing performance to reserve seed for planting, obtain BC1F2 for seed;
5) the 7-11 month in 2009: 200 strains of plantation BC1F2 plant, fruit color is separated into green and purple, therefrom selects growth, the good purple tomato individual plant of Bearing performance, carries out selfing and reserves seed for planting;
6) in December, 2009-2011 year November: obtain stable purple tomato inbred line through 4 generations of selfing continuously, therefrom select individual plant CL3-1, CL3-2 that proterties is good.
The seed selection of embodiment 4:(purple tomato CL4-1, CL4-2 inbred line)
1) in December, 2006-2007 year June: take tomato yellow 07-021 as maternal, the red tomato Zheza 204 of take is hybridized as male parent, and the F1 generation material number obtaining is 2104, and fruit color be redness;
2) the 7-11 month in 2007: take 2104 as maternal, the green tomato CH09-004 of take is hybridized as male parent, obtains triple hybrid 2104 * 09-004, and fruit color is red and yellow;
3) in December, 2007-2008 year June: take 09-004 as maternal, in 2104 * 09-004, red plant is male parent, hybridizes and obtains first backcross generation 2104 * 09-004BC1;
4) the 7-11 month in 2008: 100 strains of plantation first backcross generation plant, fruit color is separated into redness, yellow and green three kinds, therefrom selects to grow, the good green tomato individual plant selfing of Bearing performance reserves seed for planting, and obtains BC1F2 for seed;
5) 12-2009 June in 2008: 200 strains of plantation BC1F2 plant, fruit color is separated into green and purple, therefrom selects growth, the good purple tomato individual plant of Bearing performance, carries out selfing and reserves seed for planting;
6) in July, 2009-2010 year December: obtain stable purple tomato inbred line through 3 generations of selfing continuously, therefrom select individual plant CL4-1, CL4-2 that proterties is good.
Claims (1)
1. a selection for purple tomato inbred line, is characterized in that carrying out according to the following steps:
1) use the red tomato of choosing any one kind of them from T07-026, Zhejiang assorted 203 and Zheza 204 to make male parent or female parent, with the tomato yellow hybridization of choosing any one kind of them from T07-021 and CH03-056, obtaining fruit color is red F1 generation crossbreed;
2), using F1 generation crossbreed as male parent or maternal and green tomato CH09-004 hybridization, obtain fruit color for redness and yellow triple hybrid;
3) usining green tomato CH09-004 as male parent or female parent, is that red triple hybrid backcrosses and obtains backcrossing BC1 generation to fruit color;
4) BC1 is respectively red, yellow and three green types of populations for being separated into fruit color, therefrom selects the good green tomato individual plant of Agronomic to carry out selfing and reserves seed for planting, and obtains BC1F2 for seed;
5) BC1F2 is respectively colony green and purple for being separated into fruit color, therefrom selects the good purple tomato individual plant of economical character to carry out selfing and reserves seed for planting;
6) selfed seed is carried out after 3-5 isozygotys for selfing again, therefrom select to obtain the purple tomato inbred line of color, economical character stable and consistent;
Wherein, described red tomato T07-026 original number is that H24, described tomato yellow T07-021 original number are FLA456-4, from vegetables center, Asia, introduces; CH03-056 original name " yellow pearl cherry and tomato ", described green tomato CH09-004 original name " the red emerald in capital ".
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| CN104542251A (en) * | 2014-12-31 | 2015-04-29 | 安徽徽大农业有限公司 | Tomato three-way cross cultivar breeding method |
| CN104541875A (en) * | 2014-12-31 | 2015-04-29 | 安徽徽大农业有限公司 | Yellow cherry tomato scion cutting breeding method |
| CN111109074B (en) * | 2020-01-21 | 2020-12-29 | 宁波微萌种业有限公司 | Cultivation method of high-quality red-fruit tomatoes |
| CN114027172A (en) * | 2021-11-09 | 2022-02-11 | 潘捷文 | Method for cultivating new variety of wampee ginseng fruit |
| CN114027175B (en) * | 2021-11-26 | 2022-11-29 | 宁波微萌种业有限公司 | Method for cultivating high-sugar-peel thin green fruit tomato inbred line |
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| CN1910999A (en) * | 2006-08-25 | 2007-02-14 | 天津市农业生物技术研究中心 | Method for reproducing and producing hybrid seeds of tomato with crack-resistance and of pink color fruits |
| CN100353833C (en) * | 2005-07-25 | 2007-12-12 | 江苏省农业科学院 | Method for breeding seeds of hybridized tomato |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100353833C (en) * | 2005-07-25 | 2007-12-12 | 江苏省农业科学院 | Method for breeding seeds of hybridized tomato |
| CN1910999A (en) * | 2006-08-25 | 2007-02-14 | 天津市农业生物技术研究中心 | Method for reproducing and producing hybrid seeds of tomato with crack-resistance and of pink color fruits |
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