CN106665332A - Method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing dominant nuclear male sterile material - Google Patents
Method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing dominant nuclear male sterile material Download PDFInfo
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- CN106665332A CN106665332A CN201710042501.XA CN201710042501A CN106665332A CN 106665332 A CN106665332 A CN 106665332A CN 201710042501 A CN201710042501 A CN 201710042501A CN 106665332 A CN106665332 A CN 106665332A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention relates to a method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing a dominant nuclear male sterile material. The method comprises the steps of hybridizing by using the dominant nuclear male sterile material as a female parent and the water-saving and drought-resisting rice as a male parent, and backcrossing by five times, thus obtaining a water-saving and drought-resisting rice dominant nuclear male sterile plant; then hybridizing by using the water-saving and drought-resisting rice dominant nuclear male sterile plant as the female parent and 15 to 30 kinds of good parents as the male parent, and equivalently mixing offsprings to form a first round of recurrent selection populations; planting the first round of recurrent selection populations in a dry land, carrying out artificial supplementary pollination during a heading stage, selecting seeds on good sterile plants during a maturing stage, and equivalently mixing the seeds to form a second round of recurrent selection populations; repeating the recurrent selection process; selecting good fertile individual plants from the recurrent selection populations at any time, entering a pedigree method, and breeding a new variety of the water-saving and drought-resisting rice. The method for carrying out the recurrent selection breeding on the water-saving and drought-resisting rice by utilizing the dominant nuclear male sterile material, disclosed by the invention, has the advantages that the dominant nuclear male sterile material is used as a carrier, materials of the water-saving and drought-resisting rice and materials of good rice are in wide hybridization, and the genetic basis of existing water-saving and drought-resisting rice is greatly enriched.
Description
Technical field
The present invention relates to field of plant breeding, and in particular to one kind carries out saving water, resisting drought rice wheel using dominant genic male sterile material
The method for returning selection and use.
Background technology
Oryza sativa L. is typical self pollination crop, and the improvement of rice varieties for a long time relies primarily on cross breeding method,
However, limited by Traditional Man hybridization technique means and breeding cycle, merely with the parent that minority is excellent during rice breeding
Originally hybridized, cause hereditary basiies day by day narrow, the new kind being bred as is similar.With current rice breeding target not
Disconnected change, has new requirement in production to the yield of rice varieties, meter Zhi, Resistant, drought resisting and light simplified cultivation etc., traditional
Breeding method be difficult to meet the needs of agricultural production, thus break the normal procedure that to seek new approach imperative.
Saving water, resisting drought rice refers to not only the high yield and high quality characteristic with Oryza sativa L. but also one kind of the saving water, resisting drought characteristic with upland rice
New rice varieties type.It is on the basis of Oryza sativa L. scientific and technological progress, introduce upland rice saving water, resisting drought characteristic and be bred as it is new
Kind.Under irrigation conditions, its yield, meter Zhi Yu Oryza sativa L. are remained basically stable, but can economize on water more than 50%；In fields on hill tops which depend on rains for water, with preferable
To siccocolous ability（Or the basic drought-resistant ability with upland rice variety）.It is simple in cultivation, put into low, energy-conservation
Environmental protection.At present, the existing multiple kinds of saving water, resisting drought rice are deep to be liked by peasant household in the ground such as Anhui, Hubei, Guangxi popularizing planting.
2016, saving water, resisting drought rice Identification of Drought technical specification（NY/T 2863-2015）With saving water, resisting drought rice term（NY/T
2862-2015）The lasting orderly development for being embodied as saving water, resisting drought rice of the two industry standards provides powerful support.
Existing saving water, resisting drought rice varieties have wide market application foreground, in the selection-breeding of saving water, resisting drought rice new varieties from now on
In, we will continue the characteristics of keeping its drought resistance good, also need the food flavor product for further improving its diseases and insect pests resistance, rice
The character such as matter, the yield potentiality in paddy field.On the basis of existing saving water, resisting drought rice material, the high yield of Introduced into Rice breeding material,
The merits such as high-quality, Resistant, will be the main Breeding directions of saving water, resisting drought rice improvement from now on.However, drought resisting, high yield, excellent
The character such as matter and Resistant is complicated quantitative trait, is controlled by many minor genes, and these trait phenotypes identifications are complicated,
Poor repeatability between season in year, field selects difficult.Using conventional cross breeding method, to screen the list for meeting breeding objective
Strain, needs to carry out substantial amounts of hybridization and backcrossing, and carries out the phenotypic evaluation of multiple years, wastes time and energy and workload is huge, effect
Rate is low.
The content of the invention
The technical problem to be solved in the present invention is：Propose that one kind overcomes saving water, resisting drought rice material drought resistance good but yield potentiality
It is limited, and the problem that rice material yield is high but drought resistance is poor, there is provided one kind carries out samsara choosing using dominant genic male sterile material
The method for selecting breeding.
The present invention is that the technical scheme for solving above-mentioned technical problem proposition is：One kind is saved using dominant genic male sterile material
The method of water drought resisting rice recurrent selection breeding, comprises the following steps：
I, with dominant genic male sterile material as female parent, saving water, resisting drought rice is paternal hybrid and to be returned five times, obtains saving water, resisting drought rice and shows
Property Genetic Sterility material；
Ii, with the dominant genic male sterile of saving water, resisting drought rice described in step i material as it is maternal and by its respectively from different default property
15-30 rice breed of shape is hybridized for male parent, and F1 generation seed is obtained respectively；
Iii, the F1 generation seed obtained respectively in step ii is carried out into mixed in equal amounts plantation and enters it with the external world at heading stage
Row isolation, in the period of maturation seed in excellent sterile strains is harvested, and obtains the first recurrent selection population；
Iv, repeatedly by the first recurrent selection population described in step iii nonirrigated farmland plant, the time of infertility by naturally drop
Rain, at heading stage isolation processing is carried out to recurrent selection population, and the period of maturation harvests the seed in excellent sterile strains, obtains multigroup wheel
Return and select colony；
V, in the multigroup recurrent selection population obtained by step iv, picking out can meet the educated individual plant of target Drought Resistance Indexes
And into the pedigree method procedure of breeding, the water saving for meeting objective trait requirement is selected and cultivated according still further to default breeding objective
Drought resisting rice varieties.
Further, in step iv, the plantation colony of first recurrent selection population is more than 20000 plants.
Further, in step i, the saving water, resisting drought rice is that restorer drought is extensive No. 3.
Further, in step i, the saving water, resisting drought rice is conventional Rice Shanghai drought 15.
Further, in step ii, it is described respectively from the 15-30 rice breed bag with different default character
Include 75-1-127, IRBB21, CBB23, extensive 12, drought is extensive 15, drought is extensive 37, drought is extensive 49, drought is extensive 57, Fuhui 838, China account for, Cheng Hui
727th, Fujian is extensive 3301, preferably extensive, H570 and Huang Huazhan, the rice breed of China extensive 305.
Further, in step ii, it is described respectively from the 15-30 rice breed bag with different default character
Include green non-irrigated No. 1, No. 5 in Hubei Province, Huang Huazhan, Basmati, U.S. perfume (or spice) account for, NETMAT, Youzhan 8, SAGC04, middle group 14, Guangdong crystalline substance silk
Seedling, Huang Sizhan, China 1, safe Australia's silk Seedling, yellow silk osmanthus accounts for, jasmine newly accounts for, five mountain silk Seedlings, osmanthus educate No. 9, Gui Hefeng, beautiful perfume (or spice) account for,
Section educate 08, Yuejingsimiao 2, Guangdong standing grain silk Seedling, Hua Xinsi Seedlings 2, Hua Tianzhan, Guangdong agriculture silk Seedling, agriculture perfume (or spice) 32, it is yellow it is soft account for, five mountain jasmines
The rice breed of jasmine, Yuehang-1 and peasants who dig gold's silk Seedling.
The beneficial effects of the present invention is：
1st, the present invention utilizes Genetic Sterility material, overcomes artificial emasculation difficulty, makes to carry out saving water, resisting drought rice and the big rule of excellent Oryza sativa L.
Mould hybridization is possibly realized, and the hereditary basiies of current saving water, resisting drought rice breeding material so as to extreme enrichment are conducive to selecting section
Water drought resisting, the kind of high yield and high quality.Oryza sativa L. is typical self pollination crop, and breeding material carries out genetic recombination and has to carry out greatly
Amount artificial emasculation and pollinating process, need to expend a large amount of human and material resources and time.Using dominant genic male sterile material, samsara is promoted
Select to carry out extensive genetic recombination between saving water, resisting drought rice and rice breed in colony, increase excellent genes restructuring and
Polymerization, breaks the chain of unfavorable gene, so as to selection-breeding is to the saving water, resisting drought new varieties for meeting our breeding objectives.
2nd, the recurrent selection population of present invention initiative can be improved constantly.According to the new needs of Rice Production, I
Can increase the special rice genetic resource with new Breeding objective in recurrent selection population.Furthermore it is also possible to right
Recurrent selection population carries out various resistance screenings, such as recurrent selection population is planted in the sick nurseries such as rice blast, bacterial leaf spot, eliminates group
In vivo not disease-resistant individual plant, improves the overall resistance of colony, and then selects the breeding material with multi-resistance.
Specific embodiment
Embodiment one
It is restorer that the present embodiment relate to the use of dominant genic male sterile material to carry out saving water, resisting drought rice recurrent selection selection-breeding three, according to such as
Lower method is carried out：
（1）Hainan spring in 2010, with dominant genic male sterile material as female parent, extensive No. 3 of saving water, resisting drought rice restorer drought is that male parent is miscellaneous
Hand over and be returned five times, obtain the extensive No. 3 dominant genic male sterile materials of saving water, resisting drought rice drought.
（2）Shanghai Summer in 2012, with the extensive No. 3 dominant genic male sterile materials of saving water, resisting drought rice drought as female parent, respectively with 75-1-
127（pi9）, IRBB21 (Xa21), CBB23 (X23), extensive 12, drought is extensive 15, drought is extensive 37, drought is extensive 49, drought is extensive 57, Fuhui 838, China
Account for, into extensive 727, Fujian it is extensive 3301, preferably extensive, H570, Huang Huazhan and China extensive 305 have the rice breeds of merit is male parent
Hybridization, respectively obtain F1 seeds, wherein, merit be include drought resisting, blast resisting, bacterial leaf spot resistant, against-plant hopper, high-quality,
High yield, the common merit such as combining ability is good, resistant to lodging, annesl is good, nitrogen and phosphorus are efficient.
（3）Hainan spring in 2013, step（2）The middle F1 seeds for obtaining respectively carry out mixed in equal amounts plantation, and heading stage uses 2
The high thin film of rice carries out enclosing blocking isolation processing, it is ensured that intragroup pollen free pollination, the pollen for preventing external source seals in this group
In body, the seed in excellent sterile strains is harvested in the period of maturation, obtain first round recurrent selection population.
（4）Shanghai Summer in 2013, step（3）In the first recurrent selection population nonirrigated farmland plant, the time of infertility lean on nature
Rainfall, does not carry out man-made irrigation, and heading stage carries out isolation processing with thin film, and the period of maturation harvests the seed in excellent sterile strains, obtains
Obtain the second wheel recurrent selection population.
（5）2014-2016 Shanghai and Hainan, repeat step（4）3-10 time, obtain the 10th group of recurrent selection group of 3-
Body, can also be carried out 11 times as needed or more times certainly.
（6）It was found that the educated individual plant for meeting target Drought Resistance Indexes can be picked out in fourth round goes back to selection colony,
2013 summers, being planted in Guangxi Cenxi rice blast sick nursery carries out rice blast screening, the blast resisting individual plant of results, pedigree choosing
Select, plus it is stable for selfing homozygosis.To 2016, Jing added generation and test cross combination hybrid vigor identification, and numbering is 913 strain performance
The features such as drought resisting, blast resisting, good combining ability, to name as drought extensive 913.Shanghai Summer in 2016, in village row experimental station Oryza sativa L.
In variety comparative test, drought extensive 913 is shorter 2 days than compareing non-irrigated excellent 73 period of duration with the cross combination of CMS line Shanghai drought 7A institutes combo,
Yield volume increase 1%, rice blast resistance is improved.
Embodiment two
The present embodiment relates to the use of dominant genic male sterile material carries out saving water, resisting drought rice recurrent selection selection-breeding conventional Rice, according to such as lower section
Method is carried out：
（1）Hainan spring in 2010, with dominant genic male sterile material as female parent, saving water, resisting drought rice conventional Rice Shanghai drought 15 is paternal hybrid
And be returned five times, obtain saving water, resisting drought rice Shanghai 15 dominant genic male sterile materials of drought.
（2）Shanghai Summer in 2012, with saving water, resisting drought rice Shanghai 15 dominant genic male sterile materials of drought as female parent, respectively with green non-irrigated 1
Number, No. 5 in Hubei Province, Huang Huazhan, Basmati, U.S. perfume (or spice) account for, NETMAT, Youzhan 8, SAGC04, middle group 14, Yuejingsimiao, yellow silk
Account for, China 1, safe Australia's silk Seedling, yellow silk osmanthus accounts for, jasmine newly accounts for, five mountain silk Seedlings, osmanthus educate No. 9, Gui Hefeng, beautiful perfume (or spice) are accounted for, section educates 08,
Yuejingsimiao 2, Guangdong standing grain silk Seedling, Hua Xinsi Seedlings 2, Hua Tianzhan, Guangdong agriculture silk Seedling, agriculture perfume (or spice) 32, it is yellow it is soft account for, five mountain jasmines, Guangdong boat 1
Number and the rice breed with merit such as peasants who dig gold's silk Seedling be paternal hybrid, F1 seeds are obtained respectively, wherein, Optimality
Shape be include drought resisting, blast resisting, bacterial leaf spot resistant, against-plant hopper, high-quality, high yield, combining ability is good, resistant to lodging, annesl is good, nitrogen phosphorus
The common merit such as nutrient efficient.
（3）Hainan spring in 2013, by step（2）The middle F1 seeds mixed in equal amounts plantation for obtaining respectively, heading stage is with thin
Film carries out isolation processing, and the period of maturation harvests the seed in excellent sterile strains, obtains first round recurrent selection population；
（4）Shanghai Summer in 2013, step（3）In the first recurrent selection population nonirrigated farmland plant, the time of infertility lean on natural precipitation,
Man-made irrigation is not carried out.Heading stage carries out isolation processing with thin film, and the period of maturation harvests the seed in excellent sterile strains, obtains second
Wheel recurrent selection population.
（5）2014-2016 Shanghai and Hainan, repeat step（4）3-10 time, obtain the 10th group of recurrent selection group of 3-
Body, can also be carried out 11 times as needed or more times certainly.
（6）It was found that the educated individual plant for meeting target Drought Resistance Indexes can be picked out in fourth round goes back to selection colony,
2013 summers, being planted in Guangxi Cenxi rice blast sick nursery carries out rice blast screening, the blast resisting individual plant of results, pedigree choosing
Select, plus it is stable for selfing homozygosis.To 2016, Jing added generation and advantage identification, and numbering is that the strain of WF47-2 shows as drought resisting, resists
The features such as rice blast, high yield.The Funan summer of Anhui in 2016, in Funan institute of agricultural sciences saving water, resisting drought rice variety comparative test, WF47-
2 is shorter 4 days than compareing green non-irrigated No. 1 period of duration, yield volume increase 18.9%, and rice blast resistance is good.
The present invention's is not limited to above-described embodiment, and the technical scheme of above-mentioned each embodiment of the present invention can be handed over each other
Fork combination forms new technical scheme, and the technical scheme that in addition all employing equivalents are formed all falls within the guarantor of application claims
In the range of shield.
Claims (6)
1. a kind of method that utilization dominant genic male sterile material carries out saving water, resisting drought rice recurrent selection breeding, including following characteristics step
Suddenly：
I, with dominant genic male sterile material as female parent, saving water, resisting drought rice is paternal hybrid and to be returned five times, obtains saving water, resisting drought rice and shows
Property Genetic Sterility material；
Ii, with the dominant genic male sterile of saving water, resisting drought rice described in step i material as it is maternal and by its respectively from different default property
15-30 rice breed of shape is hybridized for male parent, and F1 generation seed is obtained respectively；
Iii, the F1 generation seed obtained respectively in step ii is carried out into mixed in equal amounts plantation and enters it with the external world at heading stage
Row isolation, in the period of maturation seed in excellent sterile strains is harvested, and obtains the first recurrent selection population；
Iv, repeatedly by the first recurrent selection population described in step iii nonirrigated farmland plant, the time of infertility by naturally drop
Rain, at heading stage isolation processing is carried out to recurrent selection population, and the period of maturation harvests the seed in excellent sterile strains, obtains multigroup wheel
Return and select colony；
V, in the multigroup recurrent selection population obtained by step iv, picking out can meet the educated individual plant of target Drought Resistance Indexes
And into the pedigree method procedure of breeding, the water saving for meeting objective trait requirement is selected and cultivated according still further to default breeding objective
Drought resisting rice varieties.
2. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 1, its
It is characterised by：In step iv, the plantation colony of first recurrent selection population is more than 20000 plants.
3. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 1, its
It is characterised by：In step i, the saving water, resisting drought rice is that restorer drought is extensive No. 3.
4. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 1, its
It is characterised by：In step i, the saving water, resisting drought rice is conventional Rice Shanghai drought 15.
5. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 3, its
It is characterised by：It is described to include 75-1- from the 15-30 rice breed with different default character respectively in step ii
127th, IRBB21, CBB23, extensive 12, drought is extensive 15, drought is extensive 37, drought is extensive 49, drought is extensive 57, Fuhui 838, China account for, extensive into extensive 727, Fujian
3301st, preferably extensive, H570 and Huang Huazhan, the rice breed of China extensive 305.
6. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 4, its
It is characterised by：It is described to include green non-irrigated 1 from the 15-30 rice breed with different default character respectively in step ii
Number, No. 5 in Hubei Province, Huang Huazhan, Basmati, U.S. perfume (or spice) account for, NETMAT, Youzhan 8, SAGC04, middle group 14, Yuejingsimiao, yellow silk
Account for, China 1, safe Australia's silk Seedling, yellow silk osmanthus accounts for, jasmine newly accounts for, five mountain silk Seedlings, osmanthus educate No. 9, Gui Hefeng, beautiful perfume (or spice) are accounted for, section educates 08,
Yuejingsimiao 2, Guangdong standing grain silk Seedling, Hua Xinsi Seedlings 2, Hua Tianzhan, Guangdong agriculture silk Seedling, agriculture perfume (or spice) 32, it is yellow it is soft account for, five mountain jasmines, Guangdong boat 1
Number and peasants who dig gold's silk Seedling rice breed.
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Cited By (6)
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|---|---|---|---|---|
| CN108739363A (en) * | 2018-06-22 | 2018-11-06 | 安徽袁粮水稻产业有限公司 | A kind of method of efficient selection and breeding drought resisting high-yield rice breeding material |
| CN108901823A (en) * | 2018-06-22 | 2018-11-30 | 安徽袁粮水稻产业有限公司 | A kind of method of efficient breeding low-kalium resistant high-yield rice breeding material |
| CN110140656A (en) * | 2019-06-04 | 2019-08-20 | 上海市农业生物基因中心 | A kind of recurrent breeding method for widening two-line sterile line hereditary basis |
| CN110291985A (en) * | 2019-06-04 | 2019-10-01 | 上海市农业生物基因中心 | It is a kind of improve two-line sterile line to arid, rice blast and planthopper resistance back cross breeding method |
| CN111436366A (en) * | 2020-03-25 | 2020-07-24 | 上海天谷生物科技股份有限公司 | Water-saving drought-resistant rice restorer line cultivation method |
| CN115226626A (en) * | 2022-09-02 | 2022-10-25 | 安徽省农业科学院水稻研究所 | Method for breeding hybrid rice by four lines |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739363A (en) * | 2018-06-22 | 2018-11-06 | 安徽袁粮水稻产业有限公司 | A kind of method of efficient selection and breeding drought resisting high-yield rice breeding material |
| CN108901823A (en) * | 2018-06-22 | 2018-11-30 | 安徽袁粮水稻产业有限公司 | A kind of method of efficient breeding low-kalium resistant high-yield rice breeding material |
| CN110140656A (en) * | 2019-06-04 | 2019-08-20 | 上海市农业生物基因中心 | A kind of recurrent breeding method for widening two-line sterile line hereditary basis |
| CN110291985A (en) * | 2019-06-04 | 2019-10-01 | 上海市农业生物基因中心 | It is a kind of improve two-line sterile line to arid, rice blast and planthopper resistance back cross breeding method |
| CN110140656B (en) * | 2019-06-04 | 2022-09-30 | 上海市农业生物基因中心 | Recurrent breeding method for widening genetic basis of two-line sterile line |
| CN111436366A (en) * | 2020-03-25 | 2020-07-24 | 上海天谷生物科技股份有限公司 | Water-saving drought-resistant rice restorer line cultivation method |
| CN111436366B (en) * | 2020-03-25 | 2023-05-16 | 上海天谷生物科技股份有限公司 | Water-saving drought-resistant rice restorer line cultivation method |
| CN115226626A (en) * | 2022-09-02 | 2022-10-25 | 安徽省农业科学院水稻研究所 | Method for breeding hybrid rice by four lines |
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