CN106665332B - A method of saving water, resisting drought rice recurrent selection breeding is carried out using dominant genic male sterile material - Google Patents

A method of saving water, resisting drought rice recurrent selection breeding is carried out using dominant genic male sterile material Download PDF

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CN106665332B
CN106665332B CN201710042501.XA CN201710042501A CN106665332B CN 106665332 B CN106665332 B CN 106665332B CN 201710042501 A CN201710042501 A CN 201710042501A CN 106665332 B CN106665332 B CN 106665332B
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rice
saving water
recurrent selection
resisting
resisting drought
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CN106665332A (en
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张安宁
王飞名
刘国兰
刘毅
王加红
姚文远
余新桥
罗利军
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SHANGHAI MUNICIPAL AGRICULTURAL BIOLOGICAL GENE CENTER
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to it is a kind of using dominant genic male sterile material carry out saving water, resisting drought rice recurrent selection breeding method, comprising: with dominant genic male sterile material be female parent, using saving water, resisting drought rice as male parent, hybridize and be returned five times, obtain the dominant genic male sterile strain of saving water, resisting drought rice;It then, is female parent with saving water, resisting drought rice dominant genic male sterile strain, 15-30 kind Parents form first round recurrent selection population as paternal hybrid, offspring's mixed in equal amounts;The plantation of first round recurrent selection population nonirrigated farmland, heading stage artificial supplementary pollination, maturity period select the seed in excellent sterile strains, mixed in equal amounts composition the second wheel recurrent selection population;Repeat the above recurrent selection process.Excellent fertile single plant, which is selected, from recurrent selection population at any time enters pedigree method, breeding saving water, resisting drought rice new varieties.Using dominant genic male sterile material as carrier, saving water, resisting drought rice material and excellent rice material are widely hybridized the method for the present invention, greatly enrich the hereditary basis of existing saving water, resisting drought rice.

Description

It is a kind of to carry out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material Method
Technical field
The present invention relates to field of plant breeding, and in particular to a kind of to carry out saving water, resisting drought rice wheel using dominant genic male sterile material The method for returning selection and use.
Background technique
Rice is typical self pollination crop, and the improvement of rice varieties relies primarily on cross breeding method for a long time, However, being limited by traditional artificial hybridization technique means and breeding cycle, merely with a small number of excellent parents during rice breeding This is hybridized, and causes hereditary basis increasingly narrow, and the kind being newly bred as is similar.Not with current rice breeding target It is disconnected to change, there is new requirement to the yield of rice varieties, meter Zhi, Resistant, drought resisting and light simplified cultivation etc. in production, tradition Breeding method be difficult to meet the needs of agricultural production, thus break that conventional to seek new approach imperative.
Saving water, resisting drought rice refers to the not only high yield and high quality characteristic with rice but also one kind of the saving water, resisting drought characteristic with upland rice New rice varieties type.It is to introduce the saving water, resisting drought characteristic of upland rice on the basis of rice scientific and technological progress and be bred as new Kind.Under irrigation conditions, yield, meter Zhi Yu rice remain basically stable, but can water-saving 50% or more;In fields on hill tops which depend on rains for water, have preferable Resistance arid ability (or basic drought-resistant ability with upland rice variety).It is simple and easy in cultivation, put into low, energy conservation Environmental protection.Currently, saving water, resisting drought rice has multiple kinds in the ground such as Anhui, Hubei, Guangxi popularizing planting, liked deeply by peasant household. 2016, saving water, resisting drought rice Identification of Drought technical specification (NY/T 2863-2015) and saving water, resisting drought rice term (NY/T 2862-2015) the lasting orderly development for being embodied as saving water, resisting drought rice of the two professional standards provides powerful support.
Existing saving water, resisting drought rice varieties have a vast market application prospect, in the breeding of saving water, resisting drought rice new varieties from now on In, we will continue the feature for keeping its drought resistance good, also need the food flavor product for further increasing its diseases and insect pests resistance, rice Matter, the characters such as yield potentiality in paddy field.On the basis of existing saving water, resisting drought rice material, the high yield of Introduced into Rice breeding material, The merits such as high-quality, Resistant will be the main Breeding direction of saving water, resisting drought rice improvement from now on.However, drought resisting, high yield, excellent The characters such as matter and Resistant are complicated quantitative characters, are controlled by many minor genes, and the identification of these trait phenotypes is complicated, Poor repeatability between season in year, field selection are difficult.Using conventional cross breeding method, to screen the list for meeting breeding objective Strain, needs largely to be hybridized and be returned, and carry out the phenotypic evaluation of multiple years, and time-consuming and laborious and workload is huge, imitates Rate is low.
Summary of the invention
The technical problem to be solved by the present invention is proposing that one kind overcomes saving water, resisting drought rice material drought resistance good but yield potentiality It is limited, and rice material yield is high but the problem of drought resistance difference, it provides and a kind of utilizes the progress circulation choosing of dominant genic male sterile material The method for selecting breeding.
The technical solution that the present invention proposes to solve above-mentioned technical problem is: a kind of to be saved using dominant genic male sterile material The method of water drought resisting rice recurrent selection breeding, comprising the following steps:
It i, is female parent with dominant genic male sterile material, saving water, resisting drought rice is paternal hybrid and is returned five times, obtains saving water, resisting drought Rice dominant genic male sterile material;
Ii, respectively and have different pre- with saving water, resisting drought rice dominant genic male sterile material described in step i for female parent and by it If 15-30 rice breed of character is that male parent is hybridized, F1 generation seed is obtained respectively;
Iii, by the F1 generation seed obtained respectively in step ii carry out mixed in equal amounts plantation and heading stage by its with it is outer Boundary is isolated, and the seed in maturity period harvest excellent sterile strains obtains the first recurrent selection population;
Iv, repeatedly by the first recurrent selection population described in step iii nonirrigated farmland plant, the time of infertility lean on nature Rainfall carries out isolation processing to recurrent selection population at heading stage, and the maturity period harvests the seed in excellent sterile strains, obtains multiple groups Recurrent selection population;
V, in the resulting multiple groups recurrent selection population of step iv, the fertile of target Drought Resistance Indexes can be met by picking out Single plant simultaneously enters the pedigree method procedure of breeding, is selected and cultivated to meet objective trait requirement according still further to default breeding objective Saving water, resisting drought rice varieties.
Further, in step iv, the plantation group of first recurrent selection population is at 20000 plants or more.
Further, in step i, the saving water, resisting drought rice is extensive No. 3 of restorer drought.
Further, in step i, the saving water, resisting drought rice is conventional Rice Shanghai drought 15.
Further, described respectively and with different 15-30 rice breed packets for presetting characters in step ii Include that 75-1-127, IRBB21, CBB23, extensive 12, drought are extensive 15, drought is extensive 37, drought is extensive 49, drought is extensive 57, Fuhui 838, China account for, Cheng Hui 727, extensive 3301, preferably extensive, H570 and Huang Huazhan, magnificent extensive 305 rice breed in Fujian.
Further, described respectively and with different 15-30 rice breed packets for presetting characters in step ii Include green non-irrigated No. 1, No. 5 in Hubei Province, Huang Huazhan, Basmati, U.S. perfume (or spice) account for, NETMAT, Youzhan 8, SAGC04, middle group 14, Guangdong crystalline substance silk Seedling, Huang Sizhan, China 1, safe Australia silk seedling, yellow silk osmanthus accounts for, jasmine newly accounts for, five mountains silk seedling, osmanthus educate No. 9, Gui Hefeng, beautiful perfume (or spice) account for, Section educate 08, Yuejingsimiao 2, Guangdong standing grain silk seedling, Hua Xinsi seedling 2, Hua Tianzhan, Guangdong agriculture silk seedling, agriculture perfume (or spice) 32, it is yellow it is soft account for, five mountain jasmines The rice breed of jasmine, Yuehang-1 and peasants who dig gold seedling.
The beneficial effects of the present invention are:
1, the present invention utilizes Genetic Sterility material, overcomes artificial emasculation difficulty, makes to carry out saving water, resisting drought rice and excellent rice Extensive hybridization is possibly realized, so that the extreme enrichment hereditary basis of current saving water, resisting drought rice breeding material, is conducive to breeding The kind of saving water, resisting drought, high yield and high quality out.Rice is typical self pollination crop, breeding material carry out Genetic Recombination have into The a large amount of artificial emasculations of row and pollinating process, need to expend a large amount of human and material resources and time.Using dominant genic male sterile material, push Extensive Genetic Recombination is carried out in recurrent selection population between saving water, resisting drought rice and rice breed, increases the weight of excellent genes The chain of unfavorable gene is broken in group and polymerization, so that breeding is to the saving water, resisting drought new varieties for meeting our breeding objectives.
2, the recurrent selection population that the present invention formulates can be improved constantly.According to the new needs of Rice Production, I Can increase the special rice genetic resource with new Breeding objective in recurrent selection population.Furthermore it is also possible to right Recurrent selection population carries out various resistance screenings, for example recurrent selection population is planted in the sick nurseries such as rice blast, bacterial leaf spot, eliminates group Internal not disease-resistant single plant, improves the whole resistance of group, and then selects with mostly anti-breeding material.
Specific embodiment
Embodiment one
It is restorer that the present embodiment, which relates to the use of dominant genic male sterile material to carry out saving water, resisting drought rice recurrent selection breeding three, is pressed It is carried out according to following method:
(1) 2010 year Hainan spring was female parent with dominant genic male sterile material, and saving water, resisting drought rice restorer drought extensive No. 3 are father This hybridization is simultaneously returned five times, obtains the extensive No. 3 dominant genic male sterile materials of saving water, resisting drought rice drought.
(2) 2012 years Shanghai Summers are female parent with the extensive No. 3 dominant genic male sterile materials of saving water, resisting drought rice drought, respectively with 75-1- 127(pi9), IRBB21 (Xa21), CBB23 (X23), extensive 12, drought be extensive 15, drought is extensive 37, drought is extensive 49, drought is extensive 57, Fuhui 838, China It accounts for, is extensive 3301 at extensive 727, Fujian, the preferably rice breeds of extensive, H570, Huang Huazhan and China extensive 305 with merit are male parent Hybridization, respectively obtain F1 seed, wherein merit be include drought resisting, blast resisting, bacterial leaf spot resistant, against-plant hopper, it is high-quality, The common merits such as high yield, coordinate force are good, resistant to lodging, annesl is good, nitrogen and phosphorus is efficient.
(3) 2013 year Hainan spring, the F1 seed obtained in step (2) respectively carry out mixed in equal amounts plantation, and heading stage is with 2 The high film of rice enclose blocking isolation processing, guarantees intragroup pollen free pollination, prevents the pollen of external source from sealing in this group In body, the seed in excellent sterile strains is harvested in the maturity period, obtains first round recurrent selection population.
(4) 2013 years Shanghai Summers, the first recurrent selection population is planted in nonirrigated farmland in step (3), and the time of infertility leans on nature Rainfall, without man-made irrigation, heading stage carries out isolation processing with film, and the maturity period harvests the seed in excellent sterile strains, obtains Obtain the second wheel recurrent selection population.
(5) Shanghai 2014-2016 and Hainan, repeat step (4) 3-10 times, obtain the 10th group of recurrent selection group of 3- Body can also carry out 11 times or more times as needed certainly.
(6) it finds to return in fourth round and can pick out the fertile single plant for meeting target Drought Resistance Indexes in selection group, 2013 summers were planted in Guangxi Cenxi rice blast sick nursery and carry out rice blast screening, the blast resisting single plant of harvest, pedigree choosing It selects, adds generation selfing is homozygous to stablize.To 2016, through Jia Dai and test cross combination hybrid vigour identification, the strain performance that number is 913 The features such as drought resisting, blast resisting, good coordinate force, naming is drought extensive 913.Shanghai Summer in 2016, in village row experiment station rice In variety comparative test, drought extensive 913 and the cross combination of CMS line Shanghai drought 7A institute combo are 2 days shorter than compareing non-irrigated excellent 73 breeding time, Yield volume increase 1%, rice blast resistance improves.
Embodiment two
The present embodiment relates to the use of dominant genic male sterile material and carries out saving water, resisting drought rice recurrent selection breeding conventional Rice, according to such as Lower method carries out:
In (1) 2010 year Hainan spring, be female parent with dominant genic male sterile material, and saving water, resisting drought rice conventional Rice Shanghai drought 15 is male parent Hybridize and be returned five times, obtains the non-irrigated 15 dominant genic male sterile materials in saving water, resisting drought rice Shanghai.
(2) 2012 years Shanghai Summers are female parent with the non-irrigated 15 dominant genic male sterile materials in saving water, resisting drought rice Shanghai, respectively with green non-irrigated 1 Number, No. 5 in Hubei Province, Huang Huazhan, Basmati, U.S. perfume (or spice) account for, NETMAT, Youzhan 8, SAGC04, middle group 14, Yuejingsimiao, yellow silk Account for, China 1, safe Australia silk seedling, yellow silk osmanthus accounts for, jasmine newly accounts for, five mountains silk seedling, osmanthus educate No. 9, Gui Hefeng, beautiful perfume (or spice) account for, section educates 08, Yuejingsimiao 2, Guangdong standing grain silk seedling, Hua Xinsi seedling 2, Hua Tianzhan, Guangdong agriculture silk seedling, agriculture perfume (or spice) 32, it is yellow it is soft account for, five mountain jasmines, Guangdong boat 1 Number and rice breed of peasants who dig gold's silk seedling etc. with merit be paternal hybrid, obtain F1 seed respectively, wherein Optimality Shape be include drought resisting, blast resisting, bacterial leaf spot resistant, against-plant hopper, high-quality, high yield, coordinate force is good, resistant to lodging, annesl is good, nitrogen phosphorus The common merit such as nutrient efficient.
(3) 2013 year Hainan spring planted the F1 seed mixed in equal amounts obtained respectively in step (2), and heading stage is with thin Film carries out isolation processing, and the maturity period harvests the seed in excellent sterile strains, obtains first round recurrent selection population;
(4) 2013 years Shanghai Summers, the first recurrent selection population is planted in nonirrigated farmland in step (3), and the time of infertility leans on nature Rainfall, without man-made irrigation.Heading stage carries out isolation processing with film, and the maturity period harvests the seed in excellent sterile strains, obtains Obtain the second wheel recurrent selection population.
(5) Shanghai 2014-2016 and Hainan, repeat step (4) 3-10 times, obtain the 10th group of recurrent selection group of 3- Body can also carry out 11 times or more times as needed certainly.
(6) it finds to return in fourth round and can pick out the fertile single plant for meeting target Drought Resistance Indexes in selection group, 2013 summers were planted in Guangxi Cenxi rice blast sick nursery and carry out rice blast screening, the blast resisting single plant of harvest, pedigree choosing It selects, adds generation selfing is homozygous to stablize.It to 2016, is identified through Jia Dai and advantage, the strain that number is WF47-2 shows as drought resisting, resists The features such as rice blast, high yield.The Funan summer of Anhui in 2016, in Funan institute of agricultural sciences saving water, resisting drought rice variety comparative test, WF47- 2 is 4 days shorter than compareing green non-irrigated No. 1 breeding time, and yield volume increase 18.9%, rice blast resistance is good.
Of the invention is not limited to the above embodiment, and the technical solution of above-mentioned each embodiment of the invention can be handed over each other Fork combination form new technical solution, in addition it is all using equivalent replacement formed technical solution, all fall within the present invention claims guarantor It protects in range.

Claims (4)

1. a kind of method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material, including following characteristics step It is rapid:
It i, is female parent with dominant genic male sterile material, saving water, resisting drought rice is paternal hybrid and is returned five times, and it is aobvious to obtain saving water, resisting drought rice Property Genetic Sterility material;
Ii, it is maternal with saving water, resisting drought rice dominant genic male sterile material described in step i and it is preset into property respectively and with different 15-30 rice breed of shape is that male parent is hybridized, and obtains F1 generation seed respectively;
Iii, the F1 generation seed obtained respectively in step ii is carried out mixed in equal amounts plantation and heading stage by its with the external world into Row isolation, the seed in maturity period harvest excellent sterile strains, obtains the first recurrent selection population;
Iv, repeatedly by the first recurrent selection population described in step iii nonirrigated farmland plant, the time of infertility by nature drop Rain carries out isolation processing to recurrent selection population at heading stage, and the maturity period harvests the seed in excellent sterile strains, obtains multiple groups wheel Go back to selection group;
V, in the resulting multiple groups recurrent selection population of step iv, the fertile single plant that can meet target Drought Resistance Indexes is picked out And enter the pedigree method procedure of breeding, it is selected and is cultivated to meet the water-saving of objective trait requirement according still further to default breeding objective Drought resisting rice varieties.
2. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 1, Be characterized in that: in step iv, the plantation group of first recurrent selection population is at 20000 plants or more.
3. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 1, Be characterized in that: in step i, the saving water, resisting drought rice is extensive No. 3 of restorer drought.
4. the method for carrying out saving water, resisting drought rice recurrent selection breeding using dominant genic male sterile material according to claim 1, Be characterized in that: in step i, the saving water, resisting drought rice is conventional Rice Shanghai drought 15.
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CN108901823A (en) * 2018-06-22 2018-11-30 安徽袁粮水稻产业有限公司 A kind of method of efficient breeding low-kalium resistant high-yield rice breeding material
CN108739363A (en) * 2018-06-22 2018-11-06 安徽袁粮水稻产业有限公司 A kind of method of efficient selection and breeding drought resisting high-yield rice breeding material
CN110291985A (en) * 2019-06-04 2019-10-01 上海市农业生物基因中心 It is a kind of improve two-line sterile line to arid, rice blast and planthopper resistance back cross breeding method
CN110140656B (en) * 2019-06-04 2022-09-30 上海市农业生物基因中心 Recurrent breeding method for widening genetic basis of two-line sterile line
CN111436366B (en) * 2020-03-25 2023-05-16 上海天谷生物科技股份有限公司 Water-saving drought-resistant rice restorer line cultivation method
CN115226626A (en) * 2022-09-02 2022-10-25 安徽省农业科学院水稻研究所 Method for breeding hybrid rice by four lines

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CN101218887B (en) * 2007-12-04 2010-07-14 上海市农业生物基因中心 Three-way cross combination breeding method for water-saving drought resisting hybrid rice
CN101218888B (en) * 2007-12-04 2010-06-30 上海市农业生物基因中心 Three-way cross combination propagation seed cultivating method for water-saving drought resisting hybrid rice
CN101707976B (en) * 2009-12-15 2012-07-04 福建省三明市农业科学研究所 Breeding method for converting rice target gene by utilizing Sanming dominant genic male sterile gene as carrier

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