CN108419670A - A kind of long fringe neck novel rice sterile line breeding method - Google Patents
A kind of long fringe neck novel rice sterile line breeding method Download PDFInfo
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- CN108419670A CN108419670A CN201810255072.9A CN201810255072A CN108419670A CN 108419670 A CN108419670 A CN 108419670A CN 201810255072 A CN201810255072 A CN 201810255072A CN 108419670 A CN108419670 A CN 108419670A
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- rice
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- sterile line
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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Abstract
The invention discloses a kind of selection of novel long fringe neck male sterible series of rice, which passes through use60Co gamma-rays handles the excellent existing holding based material dry seeds of character; rice shoot clump plants to obtain M2 groups after processed seed is placed 12 weeks; and the fertile mutant plants with long fringe neck character are therefrom filtered out, after clip spike of rice, moves into the field protected and carry out cutting root and stem of certain plants regeneration.It is again that female parent hybridizes with the fertile mutant plants of long fringe neck (recurrent parent) selected with the sterile line of maintainer to be mutagenic, backcross transformation obtains the excellent male sterible series of rice with long fringe neck character.The selection is while reducing mutant materials cultivated area; it can ensure that the mutant material with objective trait is not easy to be lost again; and the sterile line selected using this method can efficiently solve " packet neck " problem of male sterible series of rice; reduce the breeding cost of hybrid paddy rice; seed quality is improved, is preserved the ecological environment.
Description
Technical field
The invention belongs to Special rice crossbreeding technology fields, and in particular to a kind of novel long fringe neck rice sterile line
Selection.
Background technology
Rice is important one of the cereal crops in China, and the most of rice varieties applied in production now are hybridization water
Rice varieties, the application and popularization of hybrid paddy rice are to improving rice yield, ensureing that China's grain security has great importance.Now
Widely applied serious " packet neck " phenomenon of rice sterile line generally existing in production, sterile line " packet neck " problem restrict outer
The pollination for carrying out paternal pollen thereby reduces maternal outcrossing seed-setting rate and hybrid seed production yields.Alleviate sterile line " packet at present
There are mainly three types of the methods of neck " problem, the first is to make up sterile line GA content not by spraying " 92 〇 "
Foot adjusts effective elongation of topmost internode, and spike of rice is promoted to extract leaf sheath out, reduces the packet neck degree of sterile line, improves solid
Rate；Second is hybridized to release not with material to be rebuilt using the rice material of the long fringe neck character gene (eui genes) of carrying
It is " packet neck " problem to educate；The third is to utilize inducement of nuclear radiation breeding technique, makes the gene of the most upper panel length of adjusting and controlling rice
It mutates, selects the long-spike neck sterile line for carrying eui genes.First method is disadvantageous in that " 92 〇's "
Using breeding of hybrid rice cost can be increased, seed quality is reduced, destroys ecological environment.In addition, " 92 〇's " is excessively used
The lodging tolerance that rice can also be reduced, is unfavorable for the pollination of male parent, causes on hybrid rice seeds maturity disunity and fringe
Germination etc..Second method is disadvantageous in that the long fringe neck material of the rice remained by natural selection in nature
It is limited, so the long fringe neck material used in production now is mainly derived from inducement of nuclear radiation, make the base of control panicle neck length
Because mutating, to obtain eui genes.But due to after inducement of nuclear radiation paddy gene mutation occur at random, have
The probability very little of profit mutation generally requires to plant to obtain one plant of mutant strain with objective trait and tens of thousands of arrives millions of strains
Nuclear radiation treated plant, needs the experimental plot of several mu or even up to a hundred mu, not only time-consuming, laborious, expensive, but also operates ten
Divide cumbersome.
Invention content
(1) technical problems to be solved
In view of the deficiencies of the prior art, the technical problem to be solved in the present invention is to provide a kind of novel long fringe neck rice is male
Property sterile line breeding method, the selection can reduce the cultivated area of mutant materials, save a large amount of human and material resources
And the time, breeding cost has been saved, screening efficiency is improved.Importantly, this method can ensure there is objective trait
Mutant material is not easy to be lost, and the sterile line selected using this method can efficiently solve male sterible series of rice
" packet neck " problem.
(2) technical solution
In order to solve the above technical problem, the present invention provides such a novel long fringe neck male sterible series of rice
Selection specifically comprises the following steps：
A. the excellent maintainer seed of character is chosen, as material to be mutagenic；
B. 200-350Gy dose of radiations are used60Co gamma-ray irradiations maintainer dry seeds to be mutagenic obtain M1；
C.M1 carries out sowing seedling after placing 1-2 weeks, M1 rice shoot clumps are planted, and the seed of M1 is harvested by mixing method, obtain M2；
D.M2 is after vernalization, and uniform direct-seeding planting is in rice field one；
E. when M2 groups enter heading flowering period, by the fertile mutant plants transplanting of the long fringe neck filtered out in rice field
In two.Mutant plants pass through from generation of accompanying each other, screening, and acquisition economical character is excellent, the consistent long fringe neck strain of phenotype, as
The maintainer eB of the long-spike neck sterile line；
F. it is that female parent hybridizes with eB (recurrent parent) to utilize the sterile line of maintainer to be mutagenic in step A, backcross transformation 2-
After 3 generations, passed through Fertility identification and economical character is investigated, and be bred as the excellent male sterible series of rice eA with long fringe neck character.
Wherein, the long fertile mutant plants of fringe neck (male parent) move into the field protected after clip spike of rice in step E
It carries out cutting root and stem of certain plants regeneration, more selfed seeds can be harvested in a generation, expands targeted mutagenesis Population, obtains abundant
Mutative material.
(3) advantageous effect
Compared with prior art, the beneficial effects of the present invention are：
The selection of the present invention reduces cultivated area and screening range, saves a large amount of manpower and materials and required disappears
The time of consumption has saved selection and breeding cost, has improved screening efficiency.Meanwhile the selection can be filtered out with objective trait
Mutant plants simultaneously carry out separately protected property plantation and management, easy to be lost to make to have the material of objective trait not allowed, and cultivate
" packet neck " problem of rice sterile line can be efficiently solved by going out the long-spike neck sterile line with eui genes.In addition, passing through this hair
The sterile line of bright selection and breeding is that can lack with even without " 92 〇 " in hybrid seeding, to reduce the production of hybrid seeds of hybrid paddy rice at
This, improves seed quality, preserves the ecological environment.
In general, the selection can reduce cultivated area and screening range, save a large amount of human and material resources and
The time of required consumption has saved selection and breeding cost, improves screening efficiency, while ensureing that the material with objective trait is not allowed
It is easy to be lost, " packet neck " problem of rice sterile line can be efficiently solved.
Specific implementation mode
To make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, below it is right
Technical solution in the specific embodiment of the invention carries out clear, complete description, with the present invention is further explained, it is clear that institute
The specific implementation mode of description is only a part of embodiment of the present invention, rather than whole patterns.
Embodiment 1
About a kind of long fringe neck novel rice sterile line breeding method, specifically comprise the following steps：
A. dry using the maintainer K17B (Rice ＆ Chines Sorghum Inst., Sichuan Academy of Agricultural Sciences's selection and breeding) with Xian round-grained rice blood relationships
Seed 250-500g is as radiative material；
B. it is 200-350Gy's with dose of radiation60Co gamma-ray irradiation mutagenesis K17B dry seeds；
C. 10 days or so dry seeds M1 are placed and carry out sowing seedling, M1 kinds are planted plants dense planting plantation, population size using clump
It is 2000-3000 plants, M1 self-fertilities, by mixing method harvest seed M2；
D.M2 seeds are after vernalization, and for uniform direct-seeding planting in the rice field of about 1 mu of area, population size is ten thousand plants of 18-20；
E. fertile prominent with the long fringe neck character of high bar by what is filtered out in daily early morning in M2 groups heading flowering period
Variant plantlet of transplant is planted.Fertile mutant plants are from generation of accompanying each other, screening, the long fringe neck strain that acquired character is excellent, stable,
The as maintainer of the long-spike neck sterile line, is named as K17eB；
F. it is maternal long with high bar with sterile line K17A while the fertile mutant plants of long fringe neck in obtaining step E
Fringe neck mutant plants hybridize, and harvest 75 F1 cenospecies altogether；
G. kind plants F1, shows as male sterility, is returned by male parent and F1 of K17eB, obtains 929 BC1F1Seed；
H. kind plants BC1F1Seed, the sterile plant and recurrent parent K17eB that selection fringe neck is longer, packet neck degree reduces are returned, press
It orients single plant method and harvests BC2F1Seed；
I. strain method this plantation of list BC is pressed2F1, investigated through Fertility identification, agronomic shape in heading flowering period, screening obtains
The male sterible series of rice that neck length of spike, not packet neck or slight packet neck and character are stablized, is named as K17eA.
Embodiment 2
About a kind of long fringe neck novel rice sterile line breeding method, specifically comprise the following steps：
A. with K17B (Rice ＆ Chines Sorghum Inst., Sichuan Academy of Agricultural Sciences's selection and breeding), the early time 136B (institute of agricultural sciences of Ganzhou City choosing
Educate), the soft rice in Hunan (Hunan Province's rice is provided) be parent material, through hybridizing twice, it is (early to go back to the Hunan 136B/ to obtain K17B/
Soft rice) seed material F1；
B. kind plants F1, and selfing obtains F2；
C. kind plants F2, and orientation Single-plant selection harvest has polymerize the selfed seed of three excellent economical characters of parent, obtained
F3；
D. step C is repeated, until F6, acquired character stabilization, consistent maintainer；
E. F6 dry seeds 250g are chosen and obtain mutagenesis with the 60Co gamma-ray irradiation F6 dry seeds of 300Gy dose of radiations
Seed M1 afterwards；
F. kind plants M1, collects M1 selfed seed M2 by mixing method, obtains grain weight 7-8kg；
For G.M2 seeds after vernalization, uniform direct-seeding planting is about in 1 mu of smooth good rice field in area；
H. when M2 plant enter heading flowering period, the long fringe neck mutant plants filtered out are transplanted in daily early morning
In ready new experimental plot；
I. mutant strain is selected through inbreeding of more generation, is obtained the long fringe neck maintainer that character is consistent, stablizes, is named as emerging eB；
J. it is that female parent is hybridized with emerging eB with K17A (Rice ＆ Chines Sorghum Inst., Sichuan Academy of Agricultural Sciences's selection and breeding), obtains
To the emerging eB of K17A/；
K. respectively plant the emerging eB and emerging eB of K17A/, select with long fringe neck character the emerging eB sterile plants of K17A/ with it is emerging
EB is returned, harvest backcrossing seed BC1F1；
L. kind plants BC1F1, selects the long apparent sterile plant of fringe neck character in BC1F1 to continue to be returned with emerging eB, must be returned
Two generation seed BC2F1；
M. kind plants BC2F1, and through fertility and long fringe neck Characters Identification, continuing selection has the infertility of apparent long fringe neck character
Strain is returned with emerging eB, obtains third backcross generation's seed BC3F1；
N. kind plants BC3F1, identifies fertility, neck length of spike and the packet neck situation of plant in heading flowering period, filters out one plant of property
Shape stable and consistent, long fringe neck, the sterile plant of stable fertility, are named as emerging eA.
The selection of the present invention reduces cultivated area and screening range in the way of uniform direct-seeding planting, saves
Time of a large amount of manpower and materials and required consumption, selection and breeding cost is saved, screening efficiency is improved.Meanwhile the selection
The plant with objective trait can be filtered out, and carries out separately protected property plantation and management, to make the material with objective trait
Material is not allowed easy to be lost.The long-spike neck sterile line that this method is cultivated can effectively alleviate " packet neck " problem of rice sterile line,
It can lack during hybrid seeding and improve seed matter with even without " 92 〇 " to reduce the cost of the hybrid paddy rice production of hybrid seeds
Amount, preserves the ecological environment.
The foregoing describe the technical characteristics of the present invention and basic principle and associated advantages, for people in the art
For member, it is clear that the present invention is not limited to the details of above-mentioned exemplary embodiment, and in the design without departing substantially from the present invention
Or in the case of essential characteristic, the present invention can be realized in other specific forms.It therefore, in all respects, should all
Regard above-mentioned specific implementation mode as exemplary, and be non-limiting, the scope of the present invention is by appended claims
Rather than above description limits, it is intended that by all changes that come within the meaning and range of equivalency of the claims
It is included within the present invention.
In addition, it should be understood that although this specification is described according to each embodiment, not each embodiment
It only contains an independent technical solution, this description of the specification is merely for the sake of clarity, those skilled in the art
It should consider the specification as a whole, the technical solution in each embodiment may also be suitably combined to form this field skill
The other embodiment that art personnel are appreciated that.
Claims (2)
1. a kind of long fringe neck novel rice sterile line breeding method, which is characterized in that specifically comprise the following steps：
A. the excellent maintainer seed of character is chosen, as mutant materials；
B. 200-350Gy dose of radiations are used60Co gamma-ray irradiation maintainer dry seeds obtain M1；
C.M1 carries out sowing seedling after placing 1-2 weeks, M1 rice shoot clumps are planted, and the seed of M1 is harvested by mixing method, obtain M2；
D.M2 is after vernalization, and uniform direct-seeding planting is in rice field one；
E. when M2 groups enter heading flowering period, by the fertile mutant plants transplanting of the long fringe neck filtered out in rice field two,
Mutant plants pass through from generation of accompanying each other, screening, and acquisition economical character is excellent, the consistent long fringe neck strain of phenotype, the as long fringe
The maintainer eB of neck sterile line；
F. the sterile line of maintainer to be mutagenic in step A is utilized to hybridize with eB for female parent, then using eB as recurrent parent, backcross transformation
After 2-3 generations, passed through Fertility identification and economical character is investigated, and be bred as the excellent male sterible series of rice with long fringe neck character
eA。
2. long fringe neck novel rice sterile line breeding method according to claim 1, which is characterized in that in step E,
The long fertile mutant plants of fringe neck move into the field protected and carry out cutting root and stem of certain plants regeneration, harvest selfed seed after clip spike of rice.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810255072.9A CN108419670A (en) | 2018-03-27 | 2018-03-27 | A kind of long fringe neck novel rice sterile line breeding method |
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| CN201810255072.9A CN108419670A (en) | 2018-03-27 | 2018-03-27 | A kind of long fringe neck novel rice sterile line breeding method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109392705A (en) * | 2018-11-13 | 2019-03-01 | 华南农业大学 | The method for creating of weakly sensitivity and high fertility Oryza alta new germ plasm |
| CN111066653A (en) * | 2020-01-17 | 2020-04-28 | 江西农业大学 | Method for breeding rice three-line sterile line and maintainer line |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1198298A (en) * | 1998-06-05 | 1998-11-11 | 福建农业大学 | Selective breeding method for ear covering free male-sterile line paddy rice |
-
2018
- 2018-03-27 CN CN201810255072.9A patent/CN108419670A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1198298A (en) * | 1998-06-05 | 1998-11-11 | 福建农业大学 | Selective breeding method for ear covering free male-sterile line paddy rice |
Non-Patent Citations (1)
| Title |
|---|
| 张瑞祥等: "籼型长穗颈不育系K17eA选育", 《江西农业大学学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109392705A (en) * | 2018-11-13 | 2019-03-01 | 华南农业大学 | The method for creating of weakly sensitivity and high fertility Oryza alta new germ plasm |
| CN111066653A (en) * | 2020-01-17 | 2020-04-28 | 江西农业大学 | Method for breeding rice three-line sterile line and maintainer line |
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Application publication date: 20180821 |
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