CN103609430B

CN103609430B - Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate

Info

Publication number: CN103609430B
Application number: CN201310583567.1A
Authority: CN
Inventors: 张小明; 叶胜海; 陈蕾
Original assignee: Zhejiang Academy of Agricultural Sciences
Current assignee: Zhejiang Academy of Agricultural Sciences
Priority date: 2013-11-19
Filing date: 2013-11-19
Publication date: 2015-04-29
Anticipated expiration: 2033-11-19
Also published as: CN103609430A

Abstract

The invention relates to a method for selecting a sterile line variety of late japonica rice (Dian I type), and particularly discloses a method for breeding and reproducing a high-quality early-flowering late japonica rice sterile line Zhe japonica rice 4A with a high stigma exposure rate. The breeding method comprises the following steps of hybridizing a BT type sterile line 7627A [with the preservation number of CN1793 in the national germplasm repository and ZF02-3033 (combined into Bing 97342//Bing8502/Babawee) (with the preservation number of CGMCC No.8016), performing back crossing and selfing by using the ZF02-3033 as a male parent, performing back crossing transfer in combination with combining ability determination and high-temperature resistant identification from the BC6F1 generation, and bagging and selfing BC15F1 with the maturing rate of zero to obtain the Zhe japonica rice 4A. The late japonica rice sterile line Zhe japonica rice 4A selected by the method can early flower, and is high in stigma exposure rte, outcrossing rate, stress tolerance and quality.

Description

A kind of when spending early and the seed selection of the high late japonica rice sterile Zhejiang round-grained rice 4A of stigma exposing ratio and propagation method

Technical field

The present invention relates to the selection of a kind of late japonica rice (Yunnan I type) sterile line kind, particularly a kind of when spending early and the breeding of the high high-quality japonica rice sterile Zhejiang round-grained rice 4A in evening of stigma exposing ratio and propagation method.

Background technology

Paddy rice is the topmost cereal crops of China, and rice yield will directly affect grain security and social stability.In 20 century 70 mid-terms, the research success of hybrid rice, substantially increases the rice yield of China.The key factor that can the combination of superior hybrid crosses rice be applied on producing is hybrid seed yield, and during hybrid seed yield and sterile line colored, the characteristic such as stigma exposing ratio is closely related.It is that the general stigma exposing ratio of indica CMS line is higher that China produces three of application at present, and, hybrid seed yield is higher during flower also comparatively early; And three is that the general stigma appearing of japonica rice sterile is less, exposing ratio is lower, also comparatively slow during flower, outcrossing is poor, and hybrid seed yield is on the low side.Therefore, cultivate Hua Shizao and the high japonica rice sterile of stigma exposing ratio, for raising hybrid seed yield with to promote Japonica Hybrid Combination nova significant.

Summary of the invention

In order to solve above-mentioned technical problem, when one object of the present invention is to provide a kind of spending early and the selection of the high late japonica rice sterile Zhejiang round-grained rice 4A of stigma exposing ratio, thus cultivate Hua Shizao and the high late japonica rice sterile of stigma exposing ratio, for improve Hybrid Rice Combination hybrid seed yield and in production spread.Another object of the present invention is to provide the propagation method adopting above-mentioned late japonica rice sterile Zhejiang round-grained rice 4A.

In order to realize first above-mentioned object, present invention employs following technical scheme:

When spending early and a selection of the high late japonica rice sterile Zhejiang round-grained rice 4A of stigma exposing ratio, the method comprises the following steps:

1) select Yunnan I type sterile line 7627A to be maternal, hybridize with intermediate materials ZF02-3033 " the third 97342//the third 8502/Babawee " and obtain F ₁seed;

2) by F ₁seed presoaking and germinating, then with intermediate materials ZF02-3033 " the third 97342//the third 8502/Babawee " for male parent progressively backcrosses, in 6 generations that backcrossed, obtain BC ₆f ₁;

3) by BC ₆f ₁seed presoaking and germinating, continue with intermediate materials ZF02-3033 " the third 97342//the third 8502/Babawee " for male parent backcrosses, start in conjunction with combining ability test, high temperature resistant qualification, in 1 year two generation, carried out transformation and obtains BC simultaneously ₁₅f ₁, neat and consistent in the strain that backcrosses colony, bagging self-fruitful rate is zero, and economical character is consistent with stable fertility, cultivates late japonica rice sterile Zhejiang round-grained rice 4A.

As preferably, described step 2) sterile line and maintainer all overlap selfing bag when backcrossing at every turn, results self-fruitful rate be zero sterile line to backcross the maintainer seed of seed and corresponding bagging, guarantee next Rescued virus from generation to generation and maintainer purity.

In the breeding method of above-mentioned hybrid rice, select BT type sterile line 7627A [national genebank preservation CN1793] and fringe is large, grain is many, tillering ability is strong, Hua Shizao, angle of glume opening are large and stigma exposing ratio is high intermediate materials ZF02-3033 " the third 97342//the third 8502/Babawee " (preserving number CGMCC No.8016) hybridization, be that male parent backcrosses, from BC with ZF02-3033 again ₆f ₁in generation, starts to carry out backcross transformation in conjunction with combining ability test, high temperature resistant qualification, cultivation Hua Shizao, the sterile line that stigma exposing ratio is high, outcrossing rate good, hybrid seed yield is high.

In order to realize second above-mentioned object, present invention employs following technical scheme:

The propagation method of Zhejiang round-grained rice 4A seed, the late japonica rice sterile Zhejiang round-grained rice 4A that the method adopts claim 1 seed selection to obtain, for maternal, is male parent with intermediate materials ZF02-3033 " the third 97342//the third 8502/Babawee ", breeding Zhejiang round-grained rice 4A seed.

As preferably, do maternal with Zhejiang round-grained rice 4A, male parent is made with intermediate materials ZF02-3033 " the third 97342//the third 8502/Babawee ", sow the same period last ten-days period in May, Zhejiang round-grained rice 4A sowing quantity 1.0-1.5 kg/acre during sowing, intermediate materials ZF02-3033 " the third 97342//the third 8502/Babawee " is 0.4-0.5 kg/acre, the length of time rice seedlings grow controls at about 25 days, father, maternal row compares 2:8, female parent strain line-spacing 16.7cm × 20.0cm, originally plant for single or two, cut phase father, female parent prescinds the sword-like leave of 1/3rd simultaneously, and divide secondary to spray " 920 " 5g in the cut initial stage, first all sprays inferior to Parent during 10% plant heading, 2g/ mu, the next day, sprays for the second time, concentration doubles, only spray male parent, 3g/ mu, powder is caught up with to terminate to prescind maintainer in time afterwards.

Zhejiang round-grained rice 4A of the present invention has following beneficial effect:

1) best in quality: to analyze according to Ministry of Agriculture's rice and quality of item supervision and inspection center, according to NY/T593-2002 " edible rice varieties quality " standard, in 12 indexs of Zhejiang round-grained rice 4A sterile line inspection, protein content reaches primary standard, transparency and caustic SCC reach secondary standard, gel consistence reaches grade III Standard, and amylose content reaches four grade standards.

2) strong stress resistance: according to Zhejiang Academy of Agricultural Science plant protection and Institute of Micro-biology " 0406 " Resistance Identification group artificial infection idenfication in 2011, Zhejiang round-grained rice 4A blast resisting in seedling stage.The series of combination spontaneous induction rice blast prepared Zhejiang round-grained rice 4A at east, Tonglu brightness for 2010, shows that the combination that Zhejiang round-grained rice 4A prepares shows stronger resistance to rice blast.Within 2006 and 2007 continuous 2 years, carry out high temperature resistant process in Hainan and Hangzhou to the encirclement of Zhejiang round-grained rice 4A plastic sheeting, do not occur high temperature loose powder phenomenon, pollen fertility contrasts no significant difference with untreated, illustrates that Zhejiang round-grained rice 4A has certain heat-resisting ability.

3) outcrossing rate is high: during Zhejiang round-grained rice 4A flower early, stigma exposure rate more than 40%, and outcrossing is good, and system, breeding are all than being easier to, and output is higher.Spring in 2009, spring in 2010 and spring in 2011, setting percentage was all more than 75% in Hainan to the cross combination production of hybrid seeds that Zhejiang round-grained rice 4A configures.If research matched breeding high yield technique, is expected to obtain higher hybrid seed yield in larger area further.

Accompanying drawing explanation

Fig. 1 is breeding pedigree schematic diagram of the present invention.

Detailed description of the invention

Breeding method of the present invention is further illustrated below in conjunction with Fig. 1.

The breeding method of sterile line Zhejiang round-grained rice 4A:

A) select BT type sterile line 7627A be female parent, large with fringe, that grain is many, tillering ability is strong, Hua Shizao, angle of glume opening are large and stigma exposing ratio is high ZF02-3033 is hybridized and is obtained F ₁seed;

B) by F ₁seed presoaking and germinating, be that male parent progressively backcrosses again with ZF02-3033, when backcrossing, sterile line and maintainer all overlap selfing bag at every turn, results self-fruitful rate be zero sterile line to backcross the maintainer seed of seed and corresponding bagging, guarantee next Rescued virus from generation to generation and maintainer purity.Backcross 6 generations acquisition BC ₆f ₁;

C) by BC ₆f ₁seed presoaking and germinating, continues to be that male parent backcrosses with ZF02-3033, starts in conjunction with combining ability test, high temperature resistant qualification simultaneously, and 1 year two generation carried out transformation and obtains BC ₁₅f ₁, neat and consistent in the strain that backcrosses colony, bagging self-fruitful rate is zero, and economical character is consistent with stable fertility, cultivates compact, the of short stem dense cluster of plant type, Hua Shizao, stigma exposing ratio is high, outcrossing rate good, hybrid seed yield is high late japonica rice sterile Zhejiang round-grained rice 4A.

The propagation method of sterile line Zhejiang round-grained rice 4A:

1, desirable breeding field and suitable season is selected

Sterile line propagation field should select the field away from other paddy rice, with isolation naturally better, wind is little, on the sunny side, irrigation and drainage are convenient, soil property is good, the field of the best non-paddy rice of previous crops.Preferably stagger the florescence with other paddy rice seasonal, prevent string powder.

2, sowing time is determined

The single breeding in evening in In Hangzhou Region of Zhe Jiang Province Zhejiang round-grained rice 4A, should the end in may, and sowing Zhejiang round-grained rice 4A and ZF02-3033 the same period, under normal circumstances heading mid-September, blooms in heading limit, round-grained rice 4A limit, Zhejiang, during flower early and concentrate, the florescence is short.

3, cultivate band tiller and strengthen seedling

Zhejiang round-grained rice 4A sowing quantity 1.0-1.5 kg/acre, ZF02-3033 is 0.4-0.5 kg/acre, and rice seedling bed application rate rests in 7.5-9.0 kg/acre.The length of time rice seedlings grow controls at about 25 days.

4, rational planting proportion is determined

The rational planting proportion of Parent is: should increase maternal planting proportion to greatest extent, increases maternal effective floret bears, ensures enough pollen amounts again, improves outcrossing seed-setting rate.Determine according to test for many years, during transplanting, the ratio 2:8 of father, female parent is more satisfactory, and male parent presses seeding row spacing 16.7 cm x 20.0 centimetres, and originally single or two basis is inserted.Female parent strain line-spacing 16.7 cm x 20.0 centimetres, inserts 2, Parent spacing 24 centimetres for every clump.Put field in time, to promote to take root, fit and execute guarantor's fertilizers for potted flowers, mu executes 5 kilograms, urea, and 5 kilograms, potassium chloride, makes colony coordinate, individual healthy and strong, cultivates large fringe, improves effective floret bears.Observe in time Parent ear differentiation process in conjunction with fertilising, as there is difference, to the growth suitable many nitrogen fertilizer applications of a side faster, the many potassium applications suitable to the side that growth is slower, make the Parent florescence be in the best and to meet state.

5, artificial supplementary pollination, improves setting percentage

Father, female parent, in the cut phase, prescind the sword-like leave of 1/3rd simultaneously, make paternal pollen be easy to fly away, and improve maternal pollination rate.At the cut initial stage, a point secondary sprays " 920 " 5 grams, and during first time 10% plant heading, Parent all sprays, 2 grams/acre; The next day, sprays for the second time, and concentration doubles, and only sprays male parent, makes father, maternal heading is concentrated, neatly, elongation fringe neck, makes male parent high maternal low simultaneously.Every day bloom peak time, coordinate artificial supplementary pollination, improve outcrossing seed-setting rate, obtain higher breeding output.

6, field insect pest preventing and controlling

The control of the disease and pest such as rice blast, banded sclerotial blight, brown paddy plant hopper, leaf roll snout moth's larva, pink rice borer, striped rice borer is with general japonica rice variety.The control of stripe disease, key turns in time before surviving the winter in rice seedling bed to eliminate weeds, avoids wheatland as far as possible, and the control of nursery period reinforcement rice small brown rice planthopper, cuts off the approach that virus is propagated.

7, impurity elimination standard and technical essential

Adopt original seed level Parent seed breeding, and accomplish place isolation in 500 meters, without rice varieties.Strict impurity elimination pure keeping, guarantees seed quality, accomplish that whole breeding time all should strict impurity elimination, before and after particularly earing, strictly remove hybrid strain.First time spray " 920 " within front 1-2 days, are key periods of impurity elimination, the non-sterile strains such as the maintainer be mixed in female parent to be removed as early as possible, catch up with powder to terminate the rear maintainer that prescinds in time, gather in good time, to ensure the seed quality of sterile line.Be strictly on guard against mechanical admixture at links such as receipts, solarization, accumulatings, guarantee seed purity.

Claims

1. Hua Shizao and a selection of the high late japonica rice sterile Zhejiang round-grained rice 4A of stigma exposing ratio, is characterized in that the method comprises the following steps:

1) select Yunnan I type sterile line 7627A to be maternal, hybridize with intermediate materials " the third 97342//the third 8502/Babawee " and obtain F ₁seed;

2) by F ₁seed presoaking and germinating, then with intermediate materials " the third 97342//the third 8502/Babawee " for male parent progressively backcrosses, in 6 generations that backcrossed, obtain BC ₆f ₁, when backcrossing, sterile line and maintainer all overlap selfing bag at every turn, results self-fruitful rate be zero sterile line to backcross the maintainer seed of seed and corresponding bagging, guarantee next Rescued virus from generation to generation and maintainer purity;

3) by BC ₆f ₁seed presoaking and germinating, continue with intermediate materials " the third 97342//the third 8502/Babawee " for male parent backcrosses, start in conjunction with combining ability test, high temperature resistant qualification, in 1 year two generation, carried out transformation and obtains BC simultaneously ₁₅f ₁, neat and consistent in the strain that backcrosses colony, bagging self-fruitful rate is zero, and economical character is consistent with stable fertility, cultivates late japonica rice sterile Zhejiang round-grained rice 4A.