CN101919351A

CN101919351A - Production method of hybrid rice

Info

Publication number: CN101919351A
Application number: CN2009100869321A
Authority: CN
Inventors: 鲁梦海; 李辉
Original assignee: Beijing Jinse Nonghua Seed Science & Technology Co Ltd
Current assignee: Beijing Jinse Nonghua Seed Science & Technology Co Ltd
Priority date: 2009-06-11
Filing date: 2009-06-11
Publication date: 2010-12-22

Abstract

The invention belongs to the field of heterosis utilization, in particular relates to a production method of hybrid rice. The hybrid rice of the invention is two-line medium-indica hybrid rice which is crossbred by matching two-line sterile-line Guangzhan 63-4s and a restorer line R96, a female parent Guangzhan 63-4s is a high-yield high-quality high-resistance two-line sterile line, and a male parent R96 is bred by a selective variant in medium indica WH26. The hybrid rice has the advantages of high yield, stable yield, strong disease resistance, high biological yield, and the like.

Description

The production method of a kind of hybrid rice

Technical field

The present invention relates to the production method of a kind of hybrid rice, especially utilize two line method to cultivate the method for the new combination of hybrid rice.

Background technology

China is paddy rice big country, and rice breeding technology maintains the leading position in the world always.But because the growth of Chinese population, the food problem that reply increases population remains the problem that China's agricultural needs most solution.Produce rice paddy seed with three line method,, hindered the enthusiasm for production and the scientific research input of production company because its breeding cost is too high.Two is the production of hybrid seeds, greatly reduce production cost, greatly promoted the enthusiasm for production of production company, and at present in southern rice district main promote two to be that kind " two line system " reaches " raise two excellent No. 6 " meter matter good, the output height has obtained vast liking with kind of peasant household.Because the shortening of the alternative time limit of kind reaches in order to enlarge with planting the needs that industry company competes abroad, seed selection two is that hybrid rice seed is a most important task in present China rice breeding circle.

Summary of the invention:

The objective of the invention is to utilize the hybrid vigour theory, select high-combining ability, economical character is good, genotype is isozygotied, parents' affiliation is far away hybridization between selfed lines for use, select high yield, disease-resistant hybrid rice seed.

The invention provides a kind of method of the paddy rice cross breeding production of hybrid seeds, comprise the seed selection inbred line, isolate safely, regulate date of seeding, suitable row ratio, roguing, in good time results utilize two-line sterile line extensively to account for 63-4S and recovery is a R96 assembly hybrid rice seed.

Wherein said two-line sterile line extensively account for 63-4S be by among the variant S21-4 that extensively accounts for 63S through systematic breeding, continuously selfed breeding forms.About this male sterile line plant height 85CM, strain shape is collecting and distributing moderate, and stooling is strong, and blade is narrow, and the leaf look darker, 5 of overground part elongation internodes, and total leaf number 14-15 opens, and every total grain panicle is counted the 145-170 grain, about thousand kernel weight 25 grams.

Wherein said recovery is that R96 has found proterties variant preferably at middle Xian WH26, through 8 generations continuous selfed breeding form.

The biomaterial that the present invention relates to extensively accounts for 63, middle Xian WH26 is all open in non-patent literature.

Extensively accounting for 63 has announced in " the new variety of agricultural plant power protection communique " published on July 1st, 2002.Notification number: CNA001232E kind power number: CNA20030472.0.

Middle Xian WH26 is bulletin in " the new variety of agricultural plant power protection communique " published on March 1st, 2005.Notification number: CNA001679E kind power number: CNA 20040519.5.

It is that the hybrid rice seed wide two of R96 hybridization preparation was participated in Xian preliminary experiment in the Henan Province in excellent 96,2008 years with recovering that the present invention utilizes two-line sterile line extensively to account for 63-4S, average yield per mu 556.9kg, and comparison enters the continuous examination of succeeding year according to excellent 838 volume increase 7.1% of II.

Advantage of the present invention and good effect are:

1, output height: participated in Henan Province's test in 2008, average yield per mu 556.9kg, comparison is according to excellent 838 volume increase 7.1% of II.

2. best in quality: the outward appearance commodity grain of selling well well.

3. resistance: high resistance to hoja blanca and banded sclerotial blight.

4. wide adaptability: be adapted at Henan, the semilate rice plantation of area, the middle and lower reach of Yangtze River.

Embodiment

Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.

Embodiment 1: the concrete seed selection process of crossbreed of the present invention wide two excellent 96

Wide two excellent 96 is extensively to account for 63-4S and R96 hybridization by two-line sterile line to breed, and 6 generations of R96 is that selected plant shape is good in the variant of middle Xian WH26 the continuous menu strain of inbred line selfing are bred.Male sterile line extensively accounts for 63-4S.

Xian preliminary experiment in the Henan Province was participated in this hybrid combination in 2008, average yield per mu 556.9kg, and comparison enters the continuous examination of succeeding year according to excellent 838 volume increase 7.1% of II.

Embodiment 2: the production technology of crossbreed of the present invention wide two excellent 96

(1) safety is isolated: space length＞500 meter, or other rice varieties of heading flowering period of being separated by more than 15 days carries out the production of hybrid seeds and isolates.

(2) begin sowing in good time: first phase male parent is than maternal Zao inserting 25～30 days, or leaf order difference 6.5-7.5 leaf, or the effective accumulated temperature father and mother originally differ from, and (area is maternal in the Soviet Union sows 20-25 day in May about 205 ℃, sowing about the April 24 male parent first phase), the second phase, the third phase are separated by about 7 days with preceding first phase respectively, or are separated by 12 days with two phase male parents.

(3) rational close planting, suitable row ratio: 16.7 * 10 centimetres of seeding row spacing female parents, plant foot 15～180,000 basic seedlings for every mu, the male parent spacing in the rows is 16.7 centimetres, and the aisle was 33.3 centimetres when duplicate rows was planted, and father and mother's one's own profession ratio is 2: 20 or 1: 12.Row is to vertical with locality predominant wind at heading stage.

(4) use " 920 " technology and artificial supplementary pollination; Spray 920 during 10% female parent heading, every a spray 20 grams, the 4th spray 5 grams.Catch up with pollen to carry out artificial supplementary pollination whole flowering stage every day at florescence from fringe phase beginning to neat fringe.

(5) prevent and treat the kernel smut technology: black spirit (producer's medicine) prevents and treats kernel smut 2-3 time continuously at cut phase, fringe phase beginning and full heading time with going out.

(6) roguing technology: whole growing is pulled out form, fertility and father and mother this outside proterties and the maternal high temperature sensitive variant of removing the loose powder phenomenon among the 63-4S that extensively accounts for such as variant, contains spend back about 23 days timely results hybrid seed.

Embodiment 3 crossbreed wide two excellent 96 cultivation application technologies

(1) begins sowing in good time: at Henan area sowing on May 8, length of time rice seedlings grow 30-40 days, the rice seedling bed amount of broadcasting 1.0-1.5 kilogram/mu.

(2) transplant density and colony's requirement: make in the season rice culture and generally be advisable with 26.7 * 13.3 centimetres, basic seedling 8-10 ten thousand, plant back 15 days stem tiller numbers and reach predetermined spike number, plant and reached peak seedling in back 30 days and be no more than 270,000, become fringe 18-20 ten thousand at last, every mu of total grain husk flower 3600-4000 ten thousand, ripening rate is more than 82%, more than thousand kernel weight 24 grams.

(3) fertilizer application: always use 15 kilograms of purity nitrogens, basal plane is fertile in principle wants sufficient, and early stage, fertilizing amount accounted for always with nitrogen amount about 75%.

(4) water starch management: shallow irrigation after the rice transplanting, after stem tiller number reaches predetermined spike number, gently put several times and at last the field is baked in fact.Pustulation period is based on water, alternation of wetting and drying.Harvesting can be dewatered the last week.

(5) crossbreed can be used as rice culture in basin, the middle and lower reach of Yangtze River and the Henan area, also can be used as South China two morning in seasons of late rice.

(6) attention control rice blast and smut.

Claims

1. the production method of a hybrid rice, comprise the sowing father and mother this, spray gibberellin heading stage maternal plant, it is characterized in that: with two-line sterile line extensively account for 63-4S with recover be the seed selection of R96 group gas-mixing hybridization form two be in the Xian hybrid rice.Female parent extensively accounts for the two-line sterile line that 63-4S is the high anti-Wide-adaptive of high yield and high quality; Male parent R96 selects variant and breeds among the WH26.

2. method according to claim 1 is characterized in that safe isolation distance＞500 meter, or is separated by with this heading flowering period of described father and mother and carries out the production of hybrid seeds more than 15 days and isolate.

3. method according to claim 1, it is characterized in that first phase male parent than maternal Zao inserting 25～30 days, or leaf order difference 6.5-7.5 leaf, or the effective accumulated temperature father and mother originally differ from 205 ℃, the second phase, the third phase were separated by 7 days with preceding first phase respectively, or were separated by 12 days with two phase male parents.

4. method according to claim 1 is characterized in that 16.7 * 10 centimetres of seeding row spacing female parents, and the male parent spacing in the rows is 16.7 centimetres, and the aisle was 33.3 centimetres when duplicate rows was planted, and father and mother's one's own profession ratio is 2: 20 or 1: 12.

5. method according to claim 1 is characterized in that 10% of maternal total spike number sprays gibberellin 920 when earing, every a spray 20 grams, spray in the 4th day 5 grams.

6. method according to claim 1 and 2 is characterized in that preventing and treating kernel smut 2-3 time continuously at cut phase, fringe phase beginning and full heading time.

7. method according to claim 1 is characterized in that whole growing is pulled out outside proterties and female parent extensively accounts for the discrepant plant of 63-4S, contains and spends back 23 days results hybrid seeds.