CN102405827A - Rapid breeding method of waxy rice dual-purpose genic male sterile line - Google Patents
Rapid breeding method of waxy rice dual-purpose genic male sterile line Download PDFInfo
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- CN102405827A CN102405827A CN2011102344293A CN201110234429A CN102405827A CN 102405827 A CN102405827 A CN 102405827A CN 2011102344293 A CN2011102344293 A CN 2011102344293A CN 201110234429 A CN201110234429 A CN 201110234429A CN 102405827 A CN102405827 A CN 102405827A
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Abstract
The invention is a rapid breeding method of waxy rice dual-purpose genic male sterile lines, which is characterized in that a excellent dual-purpose genic male rice sterile line is used as an receptor parent; an excellent waxy rice variety is used as a waxy gene donor; an individual plant which is fertile in backcross progeny and exhibits glutinous-waxy separation in pollen microscopy is always used as a male parent, and is backcrossed with a recurrent parent; when the backcross progeny is quite similar to the recurrent parent, the backcross is stopped and generation addition is performed; a waxy completely-sterile plant in the backcross progeny is selected for expanding propagation and type setting. The method of the invention is more reasonable, has a high breeding speed, can increase waxy rice yield and stress resistance, has strong maneuverability, and effectively guarantees the quality of seeds.
Description
Technical field
The present invention relates to a kind of selection of rice varieties, the glutinous type dual purpose genic male sterile line of particularly a kind of paddy rice quick breeding method.
Background technology
The glutinous rice market development of China is ripe, and is comparatively concentrated, mainly be distributed in Anhui along Huaihe River one band and some areas, Hubei.Wherein market, Anhui glutinous rice seed consumption is annual more than 8,000,000 kilograms, and used kind is comparatively single, and is aging.Standardized management, the raising glutinous rice level of production and stable glutinous rice production area and output that seed selection waxy hybrid rice kind can be glutinous rice seed market provide technical guarantee, and the seed selection core of waxy hybrid rice is the seed selection of glutinous rice male sterile line.
Selection of the prior art mainly contains:
1, recessive gene backcross transformation method, classical way is the throwback backcross method in the genetics, but transformation speed is slow, and breeding cycle is long.
2, generally by sticking type and glutinous type inter breed crossing, with the breeding of pedigree back-and-forth method, this method needs colony big to the rice glutinous trait kind, and the offspring is stable slow, and breeding cycle is long.
3, molecular labeling back-and-forth method: can realize quick backcross transformation, but laboratory condition requires height, and cost is high, detection time is long.
Summary of the invention
Technical problem to be solved by this invention is the deficiency to prior art, and the glutinous type dual purpose genic male sterile line of the paddy rice quick breeding that a kind of method is more reasonable, seed selection speed is fast, cost is low method is provided.
Technical problem to be solved by this invention is to realize through following technical scheme.The present invention is the glutinous type dual purpose genic male sterile line of an a kind of paddy rice quick breeding method, is characterized in that its step is following:
(1) selects an outstanding non-glutinous type two-line sterile line,, select the good glutinous rice variety of a comprehensive proterties, make the donor parents of glutinous trait gene wx as recurrent parent; Recurrent parent is done maternal and donor parents hybridization, results first generation of hybrid seed F
1, prepare the refrigeration of capacity recurrent parent seed simultaneously, in order to backcrossing usefulness later per season;
(2) the plantation first generation of hybrid and recurrent parent seed are maternal with the recurrent parent, with first generation of hybrid hybridization, and results first backcross generation BC
1F
1
(3) plantation first backcross generation BC
1F
1With the recurrent parent seed; With 0.2 gram iodine I
2With 2 gram KI KI, add 100ml distilled water, dissolving is made into pollen staining liquid fully; Select appearance in the first backcross generation colony like recurrent parent normally can educate individual plant 10 strains, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Each 2 grain husk flowers take out wherein flower pesticide under during every fringe is got, and put on the slide, add one of pollen staining liquid, smash up flower pesticide, and inspection under 100 power microscopes is put in film-making; When pollen staining in the visual field obviously is depth separation, explain that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent and recurrent parent are hybridized again, results second backcross generation hybrid BC
2F
1
(4) plantation second backcross generation hybrid BC
2F
1With the recurrent parent seed; Repeating step (3) microscopy is selected normally can to educate and the individual plant of glutinous trait gene heterozygosis is backcrossed again produces the third backcross generation hybrid; So repetitive operation is backcrossed n for hybrid up to results, and results are backcrossed n for hybrid BC simultaneously
nF
1, n value be decided by the to backcross similarity degree of purpose and backcross progeny and recurrent parent;
(5) plantation is backcrossed n for hybrid BC
nF
1With recurrent parent; BC
nF
1Select normal fertile strain in the colony, get fringe by strain, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the individual plant of the utmost point like recurrent parent, singly receives, singly shines, singly goes out rough by strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed, sterile strain is not easy to chromoscopy under the numerous condition in south, can plant division elder generation results dry; Go out rough inspection brown rice and have or not sticking glutinous separation, eliminate not separated strain, sterile strain under positive season condition, microscopy pollen; Abortion is individual plant completely, and the regeneration plant division is received and planted, and dries, and goes out rough inspection brown rice and has or not sticking glutinous separation; Eliminate not separated strain, glutinous property brown rice is also chosen in sterile strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed;
(6) plantation BC
nF
2Each strain system, the sterile strain of glutinous property system divides system and recurrent parent to carry out the sub-district contrast, identifies that through system final election major clique, major clique are used to prepare glutinous type hybrid.
Compared with prior art, the inventive method has the following advantages and technique effect:
1, breeding speeds up: can utilize Hainan Island to add the condition in generation winter, realize backcrossing in season more than a year, the general 4-5 glutinous type isogenic line that excellent sterile is of just finalizing the design.
2, improved variety possibility increases: using this law seed selection glutinous rice male sterile line is main genetic background with the recurrent parent cell nucleus, as long as recurrent parent high yield, comprehensive proterties are good, the target male sterile line just is prone to the seed selection success.
3, implementation cost is low: this method can be omitted molecular genetic laboratory and its person skilled without Markers for Detection, as long as be equipped with microscope and relevant necessary instrument and medicament, simple to operate, cost is low, and detection speed is fast, accurately.
4, the target market is clear and definite: can carry out orderly improvement to the adaptation male sterile line in the target market, breeding objective is clear and definite, and is workable, breeds male sterile line and can promote fast.This breeding method comes down to a kind of kind quick improvement method.
Description of drawings
Fig. 1: the back cross breeding programme diagram of glutinous rice two-line sterile line.Among the figure: BCnFm representes backcross hybrid m generation the n time.
Embodiment
Following with reference to accompanying drawing, further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not constitute restriction to its right.
Embodiment 1, with reference to Fig. 1, and quick breeding glutinous rice two-line sterile line method, its step is following:
(1) selects an outstanding non-glutinous type two-line sterile line extensively to account for 63S,, select the good glutinous rice resource 148 of a comprehensive proterties, make the donor parents of glutinous trait gene wx as recurrent parent; Extensively account for 63S and do maternal and 148 hybridization, results first generation of hybrid seed F
1, prepare capacity simultaneously
Extensively account for 63SSeed refrigeration is in order to backcrossing usefulness later per season;
(2) planting the first generation of hybrid and extensively account for the 63S seed, is female parent extensively to account for 63S, with first generation of hybrid hybridization, and results first backcross generation BC
1F
1With extensively account for the 63S seed;
(3) plantation first backcross generation BC
1F
1With extensively account for the 63S seed; With 0.2 gram iodine I
2With 2 gram KI KI, add 100ml distilled water, dissolving is made into pollen staining liquid fully; Select appearance in the first backcross generation colony normally can educate individual plant 10 strains, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy like what extensively account for 63S; Each 2 grain husk flowers take out wherein flower pesticide under during every fringe is got, and put on the slide, add one of pollen staining liquid, smash up flower pesticide, and inspection under 100 power microscopes is put in film-making; When pollen staining in the visual field obviously is the depth and separates, explain that then this individual plant is the glutinous trait gene heterozygous, to select this individual plant be male parent with extensively account for 63S hybridizes results second backcross generation hybrid BC again
2F
1With extensively account for the 63S seed;
(4) plantation second backcross generation hybrid BC
2F
1With extensively account for the 63S seed; Repeating step (3) microscopy is selected normally can to educate and the individual plant of glutinous trait gene heterozygosis is backcrossed again produces the third backcross generation hybrid; So repetitive operation is backcrossed n for hybrid up to results, and results are backcrossed n for hybrid BC simultaneously
nF
1With extensively account for 63S, n value be decided by to backcross purpose and backcross progeny and extensively account for the similarity degree of 63S;
(5) plantation is backcrossed n for hybrid BC
nF
1With extensively account for 63S; BC
nF
1Select normal fertile strain in the colony, get fringe by strain, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the utmost point like the individual plant that extensively accounts for 63S, singly receives, singly shines, singly goes out rough by strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed, sterile strain is not easy to chromoscopy under the numerous condition in south, can plant division elder generation results dry; Go out rough inspection brown rice and have or not sticking glutinous separation, eliminate not separated strain, sterile strain under positive season condition, microscopy pollen; Abortion is individual plant completely, and the regeneration plant division is received and planted, and dries, and goes out rough inspection brown rice and has or not sticking glutinous separation; Eliminate not separated strain, glutinous property brown rice is also chosen in sterile strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed;
(6) plantation BC
nF
2Each strain system, the sterile strain of glutinous property is to divide system and extensively account for 63S to carry out the sub-district contrast, through system's evaluation, final election major clique, major clique are used to prepare glutinous type hybrid.
Claims (1)
1. the glutinous type dual purpose genic male sterile line of paddy rice quick breeding method is characterized in that its step is following:
(1) selects an outstanding non-glutinous type two-line sterile line,, select the good glutinous rice variety of a comprehensive proterties, make the donor parents of glutinous trait gene wx as recurrent parent; Recurrent parent is done maternal and donor parents hybridization, results first generation of hybrid seed F
1, prepare the refrigeration of capacity recurrent parent seed simultaneously, in order to backcrossing usefulness later per season;
(2) the plantation first generation of hybrid and recurrent parent seed are maternal with the recurrent parent, with first generation of hybrid hybridization, and results first backcross generation BC
1F
1
(3) plantation first backcross generation BC
1F
1With the recurrent parent seed; With 0.2 gram iodine I
2With 2 gram KI KI, add 100ml distilled water, dissolving is made into pollen staining liquid fully; Select appearance in the first backcross generation colony like recurrent parent normally can educate individual plant 10 strains, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Each 2 grain husk flowers take out wherein flower pesticide under during every fringe is got, and put on the slide, add one of pollen staining liquid, smash up flower pesticide, and inspection under 100 power microscopes is put in film-making; When pollen staining in the visual field obviously is depth separation, explain that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent and recurrent parent are hybridized again, results second backcross generation hybrid BC
2F
1
(4) plantation second backcross generation hybrid BC
2F
1With the recurrent parent seed; Repeating step (3) microscopy is selected normally can to educate and the individual plant of glutinous trait gene heterozygosis is backcrossed again produces the third backcross generation hybrid; So repetitive operation is backcrossed n for hybrid up to results, and results are backcrossed n for hybrid BC simultaneously
nF
1, n value be decided by the to backcross similarity degree of purpose and backcross progeny and recurrent parent;
(5) plantation is backcrossed n for hybrid BC
nF
1With recurrent parent; BC
nF
1Select normal fertile strain in the colony, get fringe by strain, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the individual plant of the utmost point like recurrent parent, singly receives, singly shines, singly goes out rough by strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed, sterile strain is not easy to chromoscopy under the numerous condition in south, can plant division elder generation results dry; Go out rough inspection brown rice and have or not sticking glutinous separation, eliminate not separated strain, sterile strain under positive season condition, microscopy pollen; Abortion is individual plant completely, and the regeneration plant division is received and planted, and dries, and goes out rough inspection brown rice and has or not sticking glutinous separation; Eliminate not separated strain, glutinous property brown rice is also chosen in sterile strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed;
(6) plantation BC
nF
2Each strain system, the sterile strain of glutinous property system divides system and recurrent parent to carry out the sub-district contrast, identifies that through system final election major clique, major clique are used to prepare glutinous type hybrid.
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| CN2011102344293A CN102405827A (en) | 2011-08-16 | 2011-08-16 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
| CN105475121A (en) * | 2016-01-29 | 2016-04-13 | 重庆市农业科学院 | Breeding method for high-temperature-resistant and disease-resistant rice two-line sterile line |
| WO2016118091A3 (en) * | 2015-01-21 | 2016-11-24 | The Thai Tapioca Development Institute Under The Patronage Of Hrh Princess Maha Chakri Sirindhorn | Process for improving waxy-starch cassava variety having improved qualifications and low cyanide |
| CN106942046A (en) * | 2017-03-22 | 2017-07-14 | 湖南杂交水稻研究中心 | A kind of two be the breeding method of Glutinous CMS line |
| CN109042304A (en) * | 2018-09-04 | 2018-12-21 | 江苏里下河地区农业科学研究所 | The selection of blast resisting good quality and high output two-line hybrid rice combination |
Citations (2)
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|---|---|---|---|---|
| CN101637121A (en) * | 2009-09-02 | 2010-02-03 | 连云港市农业科学院 | Method for fast selecting glutinous rice seed variety |
| CN101919351A (en) * | 2009-06-11 | 2010-12-22 | 北京金色农华种业科技有限公司 | Production method of hybrid rice |
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2011
- 2011-08-16 CN CN2011102344293A patent/CN102405827A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919351A (en) * | 2009-06-11 | 2010-12-22 | 北京金色农华种业科技有限公司 | Production method of hybrid rice |
| CN101637121A (en) * | 2009-09-02 | 2010-02-03 | 连云港市农业科学院 | Method for fast selecting glutinous rice seed variety |
Non-Patent Citations (3)
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| 王兴盛等: "回交转育水稻糯与非糯同型系育种方法的研究", 《西北农业学报》 * |
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| 黄显波等: "香型软米两系不育系明香10S选育研究", 《福建农业学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
| WO2016118091A3 (en) * | 2015-01-21 | 2016-11-24 | The Thai Tapioca Development Institute Under The Patronage Of Hrh Princess Maha Chakri Sirindhorn | Process for improving waxy-starch cassava variety having improved qualifications and low cyanide |
| CN105475121A (en) * | 2016-01-29 | 2016-04-13 | 重庆市农业科学院 | Breeding method for high-temperature-resistant and disease-resistant rice two-line sterile line |
| CN105475121B (en) * | 2016-01-29 | 2018-10-26 | 重庆市农业科学院 | A kind of selection of high temperature resistant disease resisting rice two-line sterile line |
| CN106942046A (en) * | 2017-03-22 | 2017-07-14 | 湖南杂交水稻研究中心 | A kind of two be the breeding method of Glutinous CMS line |
| CN109042304A (en) * | 2018-09-04 | 2018-12-21 | 江苏里下河地区农业科学研究所 | The selection of blast resisting good quality and high output two-line hybrid rice combination |
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Application publication date: 20120411 |