CN102405828A - Rapid breeding method of glutinous-rice sterile line of three lines - Google Patents
Rapid breeding method of glutinous-rice sterile line of three lines Download PDFInfo
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- CN102405828A CN102405828A CN2011102391773A CN201110239177A CN102405828A CN 102405828 A CN102405828 A CN 102405828A CN 2011102391773 A CN2011102391773 A CN 2011102391773A CN 201110239177 A CN201110239177 A CN 201110239177A CN 102405828 A CN102405828 A CN 102405828A
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- glutinous
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Abstract
The invention provides a rapid breeding method of a glutinous-rice sterile line of three lines. The method is characterized in that a high-quality rice maintainer line of three lines is used as a receptor parent, and high-quality glutinous rice variety is used as a glutinous gene donor; an individual plant of which the pollen is in a sticky-glutinous separation state under microscopic examination in backcross generations is used as a male parent, and the male parent and a recurrent parent are subjected to backcross; when the backcross generation is quite similar to the recurrent parent, a corresponding sterile line of the original three lines is utilized as a female parent so as to be hybridized with a glutinous plant in the backcross generations, and the glutinous plant of a higher backcross generation is continuously utilized to perform backcross so as to generate a new sterile line of three lines; and when the glutinous sterile line obtained through backcross is quite similar to the corresponding sterile line of three lines of the recurrent parent, propagation and customization are performed. By utilizing the method provided by the invention, the glutinous-rice sterile line of three lines, which can be popularized and applied in a target market, can be rapidly bred; the yield and stress resistance of glutinous rice can be improved; the glutinous rice variety can be effectively protected; and the seed quality can be ensured.
Description
Technical field
The present invention relates to a kind of seed selection method for rice, the quick breeding method of the glutinous type three line method of particularly a kind of paddy rice male sterile line.
Background technology
The glutinous rice market development of China is ripe, and is comparatively concentrated, mainly be distributed in Anhui along Huaihe River one band and some areas, Hubei.Wherein market, Anhui glutinous rice seed consumption is annual more than 8,000,000 kilograms, and used kind is comparatively single, and is aging.Standardized management, the raising glutinous rice level of production and stable glutinous rice production area and output that seed selection waxy hybrid rice kind can be glutinous rice seed market provide technical guarantee.And the seed selection core of waxy hybrid rice is the seed selection of glutinous rice male sterile line.Selection of the prior art mainly contains:
1, recessive gene backcross transformation method, classical way is the throwback backcross method in the genetics, but transformation speed is slow, and breeding cycle is long.
2, generally by sticking type and glutinous type inter breed crossing, with the breeding of pedigree back-and-forth method, this method needs colony big to the rice glutinous trait kind, and the offspring is stable slow, and breeding cycle is long.
3, molecular labeling back-and-forth method: can realize quick backcross transformation, but laboratory condition requires height, and cost is high, detection time is long.
Summary of the invention
Technical problem to be solved by this invention is the deficiency to prior art, and the quick breeding method of the glutinous type three line method of a kind of method paddy rice reasonable in design, workable, that breeding is quick, cost is low male sterile line is provided.
Technical problem to be solved by this invention is to realize through following technical scheme.The present invention is the quick breeding method of the glutinous type three line method of a kind of paddy rice male sterile line, it is characterized in that, its step is following:
(1) selecting an outstanding non-glutinous type three is maintenance line, as recurrent parent, selects the good maintenance glutinous rice variety of a comprehensive proterties, makes the donor parents of glutinous trait gene wx; Recurrent parent is done maternal and donor parents hybridization, results first generation of hybrid seed F
1, prepare the refrigeration of capacity recurrent parent seed simultaneously, in order to backcrossing usefulness later per season;
(2) the plantation first generation of hybrid and recurrent parent seed are maternal with the recurrent parent, with first generation of hybrid hybridization, and results first backcross generation BC
1F
1With the recurrent parent seed;
(3) plantation first backcross generation BC
1F
1With the recurrent parent seed; With 0.2 gram iodine I
2With 2 gram KI KI, add 100ml distilled water, dissolving is made into pollen staining liquid fully; Select seemingly individual plant 10 strains of recurrent parent of appearance in the first backcross generation colony, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Each 2 grain husk flowers take out wherein flower pesticide under during every fringe is got, and put on the slide, add one of pollen staining liquid, smash up flower pesticide, and the test under microscope of 100 times of multiplication factors is put in film-making; When pollen staining in the visual field obviously is depth separation, explain that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent and recurrent parent are hybridized again, results second backcross generation hybrid BC
2F
1With the recurrent parent seed;
(4) plantation second backcross generation hybrid BC
2F
1With the recurrent parent seed; Repeating step (3) microscopy is selected the individual plant of glutinous trait gene heterozygosis to backcross again and is produced the third backcross generation hybrid; So repetitive operation is backcrossed n for hybrid up to results, and results are backcrossed n for hybrid BC simultaneously
nF
1With recurrent parent, n value be decided by the to backcross similarity degree of purpose and backcross progeny and recurrent parent; Third backcross generation F1 adds generation one-tenth F2, goes out the plantation of the glutinous property of rough selection grain, makes male parent, is that the male sterile line work is maternal with three of recurrent parent correspondence, test cross; Per generation F1 all adds generation one-tenth F2 behind the third backcross generation, goes out the plantation of the glutinous property of rough selection grain, makes male parent, is the sterile cytoplasm donor with the test cross kind, the corresponding glutinous type isogenic line of backcross transformation recurrent parent;
(5) plantation is backcrossed n for hybrid BC
nF
1With recurrent parent; BC
nF
1Get fringe by strain in the colony, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the individual plant of the utmost point like recurrent parent, singly receives, singly shines, singly goes out rough by strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed; Continuing three in the step 4 is male sterile line transformation process;
(6) plantation BC
nF
2Each strain system, glutinous property strain branch system of system and recurrent parent carry out the sub-district contrast, identify that through system the final election major clique is shaped into glutinous type maintenance line, and three in the continuation step 4 is the male sterile line backcross process, is male sterile line until breeding stable glutinous type three; With glutinous type three is male sterile line preparation glutinous rice hybrid.
The present invention compared with prior art has the following advantages and effect.
1, breeding speeds up: can utilize Hainan Island to add the condition in generation winter, realize backcrossing in season more than a year, the general 4-5 glutinous type isogenic line that excellent sterile is of just finalizing the design.
2, improved variety is easy: using this law seed selection glutinous rice three and be male sterile line and be with the recurrent parent cell nucleus is main genetic background, as long as recurrent parent high yield, comprehensive proterties are good, the target male sterile line is seed selection easily just.
3, implementation cost is low: this method can be omitted molecular genetic laboratory and its person skilled without Markers for Detection, as long as be equipped with microscope and relevant necessary instrument and medicament, simple to operate, cost is low, and detection speed is fast, accurately.
4, the target market is clear and definite: can carry out orderly improvement to the adaptation male sterile line in the target market, breeding objective is clear and definite, and is workable, breeds male sterile line and can promote fast.
Description of drawings
Fig. 1 is a back cross breeding programme diagram of the present invention; BC among the figure
nF
mRepresent backcross hybrid m generation the n time.
Embodiment
Following with reference to accompanying drawing, further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not constitute restriction to its right.
Embodiment 1, and with reference to Fig. 1, quick breeding glutinous rice three is the male sterile line method, and its step is following:
(1) selecting an outstanding non-glutinous type three is the safe B in maintenance line Guangdong, as recurrent parent, selects the good maintenance glutinous rice variety 148 of a comprehensive proterties, makes the donor parents of glutinous trait gene wx; The safe B in Guangdong does maternal and 148 hybridization, results first generation of hybrid seed F
1With the safe B seed in Guangdong;
(2) the safe B seed in the plantation first generation of hybrid and Guangdong, safe B is a female parent with Guangdong, with first generation of hybrid hybridization, results first backcross generation BC
1F
1With the safe B seed in Guangdong;
(3) plantation first backcross generation BC
1F
1With the safe B seed in Guangdong; With 0.2 gram iodine I
2With 2 gram KI KI, add 100ml distilled water, dissolving is made into pollen staining liquid fully; Select seemingly individual plant 10 strains of the safe B in Guangdong of appearance in the first backcross generation colony, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Each 2 grain husk flowers take out wherein flower pesticide under during every fringe is got, and put on the slide, add one of pollen staining liquid, smash up flower pesticide, and the test under microscope of 100 times of multiplication factors is put in film-making; When pollen staining in the visual field obviously is depth separation, explain that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that the safe B in male parent and Guangdong is hybridized again, results second backcross generation hybrid BC
2F
1The safe B seed in seed and Guangdong;
(4) plantation second backcross generation hybrid BC
2F
1With the safe B seed in Guangdong; Repeating step (3) microscopy is selected the individual plant of glutinous trait gene heterozygosis to backcross again and is produced the third backcross generation hybrid; So repetitive operation is backcrossed n for hybrid up to results, and results are backcrossed n for hybrid BC simultaneously
nF
1With the safe B in Guangdong, n value be decided by the to backcross similarity degree of purpose and backcross progeny and Guangdong Thailand B; Third backcross generation F1 adds generation one-tenth F2, goes out the plantation of the glutinous property of rough selection grain, makes male parent, and is maternal with the safe A work in Guangdong, test cross; Per generation F1 all adds generation one-tenth F2 behind the third backcross generation, goes out the plantation of the glutinous property of rough selection grain, makes male parent, is the sterile cytoplasm donor with the test cross kind, the corresponding glutinous type isogenic line of the safe B in backcross transformation Guangdong;
(5) plantation is backcrossed n for hybrid BC
nF
1With the safe B in Guangdong; BC
nF
1Get fringe by strain in the colony, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the individual plant of the utmost point like the safe B in Guangdong, singly receives, singly shines, singly goes out rough by strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed; Continuing three in the step 4 is male sterile line transformation process;
(6) plantation BC
nF
2Each strain system, glutinous property strain branch system of system and the safe B in Guangdong carry out the sub-district contrast, and through system's evaluation, the final election major clique is shaped into glutinous type maintenance line, and continuing three in the step 4 is the male sterile line backcross process, until breeding stable glutinous type Guangdong Thailand A; With the safe A preparation in glutinous type Guangdong glutinous rice hybrid.
Claims (1)
1. the quick breeding method of the glutinous type three line method of a paddy rice male sterile line is characterized in that its step is following:
(1) selecting an outstanding non-glutinous type three is maintenance line, as recurrent parent, selects the good maintenance glutinous rice variety of a comprehensive proterties, makes the donor parents of glutinous trait gene wx; Recurrent parent is done maternal and donor parents hybridization, results first generation of hybrid seed F
1, prepare the refrigeration of capacity recurrent parent seed simultaneously, in order to backcrossing usefulness later per season;
(2) the plantation first generation of hybrid and recurrent parent seed are maternal with the recurrent parent, with first generation of hybrid hybridization, and results first backcross generation BC
1F
1With the recurrent parent seed;
(3) plantation first backcross generation BC
1F
1With the recurrent parent seed; With 0.2 gram iodine I
2With 2 gram KI KI, add 100ml distilled water, dissolving is made into pollen staining liquid fully; Select seemingly individual plant 10 strains of recurrent parent of appearance in the first backcross generation colony, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Each 2 grain husk flowers take out wherein flower pesticide under during every fringe is got, and put on the slide, add one of pollen staining liquid, smash up flower pesticide, and the test under microscope of 100 times of multiplication factors is put in film-making; When pollen staining in the visual field obviously is depth separation, explain that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent and recurrent parent are hybridized again, results second backcross generation hybrid BC
2F
1With the recurrent parent seed;
(4) plantation second backcross generation hybrid BC
2F
1With the recurrent parent seed; Repeating step (3) microscopy is selected the individual plant of glutinous trait gene heterozygosis to backcross again and is produced the third backcross generation hybrid; So repetitive operation is backcrossed n for hybrid up to results, and results are backcrossed n for hybrid BC simultaneously
nF
1With recurrent parent, n value be decided by the to backcross similarity degree of purpose and backcross progeny and recurrent parent; Third backcross generation F1 adds generation one-tenth F2, goes out the plantation of the glutinous property of rough selection grain, makes male parent, is that the male sterile line work is maternal with three of recurrent parent correspondence, test cross; Per generation F1 all adds generation one-tenth F2 behind the third backcross generation, goes out the plantation of the glutinous property of rough selection grain, makes male parent, is the sterile cytoplasm donor with the test cross kind, the corresponding glutinous type isogenic line of backcross transformation recurrent parent;
(5) plantation is backcrossed n for hybrid BC
nF
1With recurrent parent; BC
nF
1Get fringe by strain in the colony, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the individual plant of the utmost point like recurrent parent, singly receives, singly shines, singly goes out rough by strain, selects wherein glutinous property grain to be mixed into the n that backcrosses for second generation of hybrid BC
nF
2Seed; Continuing three in the step 4 is male sterile line transformation process;
(6) plantation BC
nF
2Each strain system, glutinous property strain branch system of system and recurrent parent carry out the sub-district contrast, identify that through system the final election major clique is shaped into glutinous type maintenance line, and three in the continuation step 4 is the male sterile line backcross process, is male sterile line until breeding stable glutinous type three; With glutinous type three is male sterile line preparation glutinous rice hybrid.
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| CN2011102391773A CN102405828A (en) | 2011-08-19 | 2011-08-19 | Rapid breeding method of glutinous-rice sterile line of three lines |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210844A (en) * | 2013-04-23 | 2013-07-24 | 江苏徐淮地区徐州农业科学研究所 | Method for improving frequency of callus induction and plant differentiation rate in japonica rice anther culture |
| CN103314843A (en) * | 2013-07-11 | 2013-09-25 | 江西省农业科学院水稻研究所 | Method for quickly cultivating novel cytoplasm male sterility line of rice |
| CN104026002A (en) * | 2014-06-03 | 2014-09-10 | 江苏徐淮地区连云港农业科学研究所 | Quick cultivation method of sterile line of three-line hybrid rice |
| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185898A (en) * | 1997-09-10 | 1998-07-01 | 武汉大学 | Selection of sterility lined with leaf marks for three way crossed rice |
| CN1618274A (en) * | 2003-11-18 | 2005-05-25 | 湖南杂交水稻研究中心 | Method for increasing prodn. of hybridized rice |
| CN101637121A (en) * | 2009-09-02 | 2010-02-03 | 连云港市农业科学院 | Method for fast selecting glutinous rice seed variety |
-
2011
- 2011-08-19 CN CN2011102391773A patent/CN102405828A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185898A (en) * | 1997-09-10 | 1998-07-01 | 武汉大学 | Selection of sterility lined with leaf marks for three way crossed rice |
| CN1618274A (en) * | 2003-11-18 | 2005-05-25 | 湖南杂交水稻研究中心 | Method for increasing prodn. of hybridized rice |
| CN101637121A (en) * | 2009-09-02 | 2010-02-03 | 连云港市农业科学院 | Method for fast selecting glutinous rice seed variety |
Non-Patent Citations (1)
| Title |
|---|
| 王兴盛等: "回交转育水稻糯与非糯同型系育种方法的研究", 《西北农业学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210844A (en) * | 2013-04-23 | 2013-07-24 | 江苏徐淮地区徐州农业科学研究所 | Method for improving frequency of callus induction and plant differentiation rate in japonica rice anther culture |
| CN103314843A (en) * | 2013-07-11 | 2013-09-25 | 江西省农业科学院水稻研究所 | Method for quickly cultivating novel cytoplasm male sterility line of rice |
| CN104026002A (en) * | 2014-06-03 | 2014-09-10 | 江苏徐淮地区连云港农业科学研究所 | Quick cultivation method of sterile line of three-line hybrid rice |
| CN104026002B (en) * | 2014-06-03 | 2016-05-18 | 江苏徐淮地区连云港农业科学研究所 | A kind of fast breeding method of series of three-series hybrid rice sterile line |
| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
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Application publication date: 20120411 |