CN105145334A - Seed selection method of new rice species - Google Patents
Seed selection method of new rice species Download PDFInfo
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- CN105145334A CN105145334A CN201510406665.7A CN201510406665A CN105145334A CN 105145334 A CN105145334 A CN 105145334A CN 201510406665 A CN201510406665 A CN 201510406665A CN 105145334 A CN105145334 A CN 105145334A
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Abstract
The invention provides a seed selection method of a new rice species. The new hsien rice species is obtained through two line hybridization of indica type light and temperature sensitive nuclear sterile line 602S as a female parent and restoring line R5549 as a male parent, wherein the female parent 602S is a two line sterile line obtained after hybridization of Guangzhan 63S as a female parent and early indica R402 as a female parent and 6 generation pedigree selection breeding; and the male parent R5549 is a medium indica restoring line obtained after hybridization of Huahui 118 as a female parent and Gui 99-2 as a male parent and 8 generation pedigree selection breeding. The method has the characteristics of high seed output, high hybrid rice output, and high quality and strong resistance of rice, and the obtained two line hybrid hsien rice resists lodging, has green stalks, yellow seeds and purple leaf sheath and grain tip, and is a species easily discriminated from abnormal plants.
Description
Technical field
Variety of crops breeding technique field of the present invention, is specifically related to a kind of selection of new rice variety.
Background technology
China is main Rice Production big country of the world, it is 18,100 ten thousand tons that national paddy in 2006 always produces, account for Grain in China and always produce 36.4%, account for that world's paddy always produces 30%, rice industry is also the advantage leading industry of our province agricultural, only northern Suzhou hybrid rice cultivated area is just more than 3,000,000 mu, but the New Hybrid Rice Combination of high yield and high quality resistance is little, be urgent need to solve the problem national hybrid rice, the indica type light temperature sensitive dual purpose genic male sterile line seed selection success that particularly high-quality resistance risk in hybrid seed production is little is very difficult.According to Ministry of Agriculture's statistics display, the popularizing planting area of 2006-2008 double-linear hybrid rice is every year on average with the speed increase of 66.67 ten thousand hectares, the national double-linear hybrid rice cultivated area 475.7 ten thousand hectares to 2008, account for hybrid rice cultivated area 30%, the another great agricultural science and technology chief creating new results of China after series of three-series hybrid rice, for improving rice in China per unit area yield further, providing fine quality rice and ensureing that national food security has made significant contribution.
But along with the continuous expansion of double-linear hybrid rice cultivated area, its limitation on producing also displays gradually, and the purification breeding problem of such as male sterile line, male sterile line is produced by kind of an efficiency of supply problem, production of hybrid seeds safety problem etc.And cause the basic reason of these problems to be that the male sterility of photo-thermo sensitive male nuclear sterile rice line has the advantages that to fluctuate with the change of the ecological factors such as the long temperature of light, add that in same male sterile line colony, between Different Individual, Fertility al-teration origin temp there are differences, make photo-thermo-sensitive genic male sterile line after breeding some generations continuously, colony's Fertility al-teration origin temp is easily caused to raise, in hybrid seeding, be subject to low temperature effect and self-fertility loses Commercial cultivation value, this is the huge potential risk existed during double-linear hybrid rice seeds is produced.At present, in production, this Fertility al-teration origin temp " drift " phenomenon of rice photo-thermo-sensitive dual purpose genic male sterile line is very outstanding.
Mostly the hybrid rice variety that current production is planted, be series of three-series hybrid rice kind.Double-linear hybrid rice output, meter Zhi, anti-fall and resistance do very well, but double-linear hybrid rice also exists the risk of the production of hybrid seeds.Though and there is not the risk of the production of hybrid seeds in wild abortion type series of three-series hybrid rice, but poor in its meter of matter.
Summary of the invention
The shortcoming of, early ageing poor for existing two-line hybrid rice lodging resistance and object innovation difficulty, the present invention aim to provide a kind of anti-fall, the blue or green seed of stalk is yellow and be easy to distinguish the two-line hybrid rice kind of hybrid strain at different times.
The present invention realizes especially by following technical scheme:
The present invention is maternal by doing with indica type photo-thermo-sensitive genic male sterile line 602S, the two-line early hybrid rice rice new varieties of male parent preparation are made with restorer R5549, wherein maternal 602S makes female parent with Guangzhan 63S, makes paternal hybrid with Xian R402 morning, through the two-line sterile line that the pedigree selection of 6 generations is bred as; Male parent R5549 does maternal with China extensive 118, and osmanthus 99-2 makes paternal hybrid, Xian restorer in the pedigree selection of 8 generations is bred as.
The selection of a kind of new rice variety of the present invention, with indica type photo-thermo-sensitive genic male sterile line 602S for female parent, with restorer R5549 for male parent, comprises the following steps:
1) seed selection of maternal male sterile line 602S.
First Year is with the temperature sensitive dual purpose genic male sterile line Guangzhan 63S of indica type light for female parent, and quality early rice R402 is paternal hybrid combo, obtains crossbreed 62; Winter in the same year Hainan plantation F1, performance plant and leaf shape is good, it is delicate and pretty, normally solid to grow, and mixes sowing time ripe;
Second Year positive season plantation F2, selects the good sterile strain of Comprehensive Traits to cut blue or green regeneration, selects 20 seeds regenerating individual plant time ripe during heading; Winter in the same year Hainan plantation F3, time ripe, totally 20 strain fecundities are good in choosing, and strain Leaf pattern is good, the single-strain seed that Comprehensive Traits is excellent;
3rd year positive season plantation F4, the strain microscopy screening that the thorough strain Leaf pattern of abortion is good is selected during heading, the excellent sterile strain of Comprehensive Traits is selected to raise extensive 527 test crosses in restorer another name for Sichuan Province of rice No. 6 and the seed selection of paddy rice institute of Sichuan Agricultural University with the restorer of the lane housing institute of agricultural sciences of Jiangsu Province seed selection respectively, all the other cut blue or green regeneration and shading treatment is reserved seed for planting, screen through Identification of Comprehensive Characters, retain the sterile strain of 4 Comprehensive Traits excellences, and select this 4 strain regeneration single-strain seeds respectively; F5 is for selected 4 strains and test cross kind thereof for Hainan plantation of winter in the same year, in 4 sterile strains, selects general performance to give prominence to strain HS602 and test cross offspring thereof, selects excellent sterile individual plant further;
Within 4th year, plantation F6 is for the sterile individual plant of selected excellence, respectively again with restorer R5549, raise extensive 527 test crosses of rice No. 6 and another name for Sichuan Province, obtain test cross kind HS602/R5549, HS602/ and raise rice No. 6, HS602/ another name for Sichuan Province extensive 527, retain overall strain abortion thoroughly without the strain of pollen, cut blue or green regeneration and shading treatment is reserved seed for planting respectively; Winter in the same year Hainan plantation F7 raises rice No. 6, extensive 527 qualifications in HS602/ another name for Sichuan Province for strain selected by HS602 and test cross kind HS602/R5549, HS602/, to the strain of HS602 menu, and roguing system seed.
2) restorer R5549 seed selection.
First Year does female parent with China extensive 118, and osmanthus 99-2 makes paternal hybrid, winter in the same year Hainan plantation F1, plant and leaf shape is good, grow that delicate and pretty, solid normal mature seed is mixed to be received;
Second Year positive season plantation F2, colony's performance point from, select 7 leaf sheaths and seed thin membrane inside rush stalk point to be separate targets individual plant winter in the same year Hainan plantation F3 of purple;
10 target individual plants that 3rd year spring selected in the strain of Hainan 205 that Comprehensive Traits is good, leaf sheath and seed thin membrane inside rush stalk point are purple, positive season plantation in the same year F4 eliminates a strain in 10 strains, the menu strain respectively of residue strain, totally 9 individual plants;
Hainan, 4th year spring Hainan plantation F5 is for selected 9 individual plants, and positive season plantation in the same year F6, selects Comprehensive Traits to stablize, show excellent target strain 5; In Hainan breeding F7 generation winter in the same year, continue to select Comprehensive Traits to show excellent individual plant respectively in above-mentioned 5 strains;
Within 5th year, positive season breeding individual plant F8 shows excellent individual plant for above-mentioned 5 strain Comprehensive Traits, and be measured with II-32A, Xieqingzao A and Peiai 64S respectively, Hainan breeding of winter in the same year, and qualification is measured advantage, obtain strain 5549 combos conjunction ripening rates high, high yield and high quality, strain 5549 i.e. restorer R5549.
3) with indica type photo-thermo-sensitive genic male sterile line 602S for female parent, restorer R5549 is male parent combo seeding.
Seed production way in step of the present invention (3) is specially: Parent differs from 41 days according to broadcasting (maternal 602S broadcasts 79 days phases beginning, R5549 broadcasts 120 days phases beginning), leaf differs from 8.2 leaves (maternal 602S leaf number of the main culm 14.5 leaf, male parent R5549 leaf number of the main culm 18.5 leaf), the wide 210cm in railway carriage or compartment, row (male parent 1 row more alternate with 1:10-12, maternal 10-12 is capable) plantation, meet when ensureing Parent florescence flower in the safe fertility alteration period of maternal 602S, carry out 602S/R5549 (China two excellent 69) hybrid seeding.
Beneficial effect of the present invention is: breeding method of the present invention overcomes the risk that existing double-linear hybrid rice also exists the production of hybrid seeds, there is hybrid seed yield high, the features such as hybrid rice output is high, rice is of fine quality, resistance, simultaneously the inventive method gained two-line hybrid rice is anti-fall, the blue or green seed of stalk is yellow and kind leaf sheath and seed inspire confidence in point and be purple, is easy at different times the kind distinguishing hybrid strain.
Accompanying drawing explanation
Fig. 1 is that breeding system spectrogram of the present invention shows.
Embodiment
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, any simple modification done following examples according to technical spirit of the present invention or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
As shown in Figure 1, China two excellent 69 makes female parent with indica type photo-thermo-sensitive genic male sterile line 602S, makes the two-line early hybrid rice rice new varieties of male parent preparation with restorer R5549.Wherein maternal 602S makes female parent with Guangzhan 63S, makes paternal hybrid with Xian R402 morning, through the two-line sterile line that the pedigree selection of 6 generations is bred as; Male parent R5549 does maternal with China extensive 118, and osmanthus 99-2 makes paternal hybrid, Xian restorer in the pedigree selection of 8 generations is bred as.
The seed selection of maternal male sterile line 602S: summer calendar year 2001 in Hu Xi farm, Pei County, Jiangsu Province institute of agricultural sciences experimental field with the temperature sensitive dual purpose genic male sterile line Guangzhan 63S of indica type light for female parent, quality early rice R402 is paternal hybrid combo, obtain crossbreed 62, winter in the same year goes to breeding base, Hainan to plant F1, performance plant and leaf shape is good, it is delicate and pretty, normally solid to grow, and mixes sowing time ripe, positive season in 2002 is at Hu Xi farm, Pei County, Jiangsu Province plantation F2, colony's performance Fertility segregation, about there are 1/3rd plant performances sterile, the good sterile strain of Comprehensive Traits is selected to cut blue or green regeneration during heading, select 20 seeds regenerating individual plant time ripe, the winter in the same year goes to breeding base, Hainan to plant F3, selects 20 strain fecundities good time ripe, strain Leaf pattern is good, the single-strain seed that Comprehensive Traits is excellent, positive season in 2003 is at Hu Xi farm, Pei County, Jiangsu Province plantation F4, colony continues to occur that sterile strain is separated, the strain microscopy screening that the thorough strain Leaf pattern of abortion is good is selected during heading, select the excellent sterile strain of Comprehensive Traits respectively with raise the restorer test crosses such as rice No. 6, all the other cut blue or green regeneration and shading treatment is reserved seed for planting, time ripe, HS602 is received in choosing, HS606, 4 strains of HS609 and HS609, and select 4 regeneration single-strain seeds respectively, winter in the same year goes to breeding base, Hainan to plant F5 strain and test cross kind thereof, colony shows basic neat and consistent, wherein give prominence to HS602 strain and test cross offspring general performance thereof, in the strain of HS602 strain menu, positive season in 2004 for individual plant selected by HS602, respectively again with restorer R5549 with raise rice No. 6 test cross of etc.ing, retains overall strain abortion thoroughly without the strain of pollen at Hu Xi farm, Pei County, Jiangsu Province plantation F6, cuts green grass or young crops and to regenerate and shading treatment is reserved seed for planting respectively, winter in 2004 at Hainan plantation F7 for strain selected by HS602 and the qualification of test cross kind thereof, menu strain, roguing system seed, winter in 2004 hybrid rice new combination qualification on Hu Xi farm, Pei County, Jiangsu in breeding base, Hainan and positive season in 2005 of Jiangsu Province great Hua Zhong industry group company tissue is participated in HS602 × R5549 combination, and HS602 × R5549 performance tillering ability is strong, the tight Minato of plant type, plant and leaf shape good, the ripe phase is moderate, disease resistance strong, big panicle many grains per panicle ripening rate is high, rice is of fine quality, Comprehensive Traits is outstanding.HS602 tentatively titled 602S.
Restorer R5549 seed selection: do female parent on Hu Xi farm, Pei County, Jiangsu Province with China extensive 118 in 1999, osmanthus 99-2 (source: osmanthus 99 × raise rice No. 6) makes paternal hybrid, winter in the same year is in breeding base, Hainan plantation F1 generation, good by plant and leaf shape, grow that delicate and pretty, solid normal mature seed is mixed to be received; Positive season in 2000 is in Hu Xi farm, Pei County, Jiangsu Province plantation F2 generation, colony's performance point from, select 201,202,203 ... 207 totally 7 plant and leaf shapes good, grow delicate and pretty, leaf sheath and seed thin membrane inside rush stalk point is purple, solid normal separate targets individual plant, the winter in 2000 plants F3 in Hainan; 10 target individual plants 2051,2052,2053 that spring calendar year 2001 selects in the strain of Hainan 205 that Comprehensive Traits is good, leaf sheath and seed thin membrane inside rush stalk point are purple ... 2060; Calendar year 2001 in positive season, at Hu Xi farm, Pei County, Jiangsu plantation F4, is selected 2055 strains, in 2055 strains, selects 205541,205542,205543 ... 205549 good, the leaf sheath of totally 9 Comprehensive Traits and seed thin membrane inside rush stalk point are the target individual plant of purple; Spring in 2002 is in Hainan plantation F5 generation, eliminate strain 205543 and 205547, residue strain selects that Comprehensive Traits is good respectively again, leaf sheath and seed thin membrane inside rush stalk point are purple target individual plant 5541,5542,5544,5545,5546,5548 and 5549, selects 7 target individual plants altogether; Positive season in 2002 is in Hu Xi farm, Pei County, Jiangsu Province plantation F6 generation, Comprehensive Traits is selected to stablize, show excellent, leaf sheath and seed thin membrane inside rush stalk point are the target strain 5541 (precocious high stalk) of purple, 5542 (long awns), 5544 (fringe type is long, Spikelet density is little), 5548 (precocious of short stem) and totally 5,5549 (short awns); Winter is in Hainan breeding F7 generation then, continues to select respectively Comprehensive Traits to show the target individual plant that excellent, leaf sheath and seed thin membrane inside rush stalk point are purple in 5 strains; Within 2003, positive season F8 is in the breeding of Hu Xi farm, Pei County, Jiangsu Province and qualification target strain 5549,5542,5544,5548 and 5549, and is measured with II-32A, Xieqingzao A and Peiai 64S respectively; In Hainan breed then by the winter for the target individual plant 5549 that Comprehensive Traits excellent, the leaf sheath of performance and seed thin membrane inside rush stalk point are purple, and identify that it is measured advantage, and 5549 combos are closed and resisted, and disease-resistant, ripening rate is high, high yield and high quality; Positive season in 2004 is measured with two-line sterile line 602S on Hu Xi farm, Pei County, Jiangsu Province 5549; Winter in 2004, in qualification hybrid 602S × 5549, Hainan, shows the feature of high yield and high quality resistance; Carry out 5549 in positive season in 2005 manufacture experimently kind with 602S on Hu Xi farm, Pei County, Jiangsu Province, restorer is named as R5549, and the excellent every 667m2 output of the production of hybrid seeds of 5549, namely magnificent two excellent 69 of the magnificent Xian of preparation combination is at about 250 kilograms.
China two excellent 69 participates in Jiangsu Province's regional test in 2008-2009, and within 2010, participate in Jiangsu Province's production test, 2011 by Jiangsu Province's variety certification.
Claims (4)
1. the selection of a new rice variety, it is characterized in that: do maternal with indica type photo-thermo-sensitive genic male sterile line 602S, the two-line early hybrid rice rice new varieties of male parent preparation are made with restorer R5549, wherein maternal 602S makes female parent with Guangzhan 63S, paternal hybrid is made, through the two-line sterile line that the pedigree selection of 6 generations is bred as with Xian R402 morning; Male parent R5549 does maternal with China extensive 118, and osmanthus 99-2 makes paternal hybrid, Xian restorer in the pedigree selection of 8 generations is bred as.
2. the selection of a kind of new rice variety according to claim 1, is characterized in that: the seed selection of maternal male sterile line 602S is specially:
First Year is with the temperature sensitive dual purpose genic male sterile line Guangzhan 63S of indica type light for female parent, and quality early rice R402 is paternal hybrid combo, obtains crossbreed 62; Winter in the same year Hainan plantation F1, performance plant and leaf shape is good, it is delicate and pretty, normally solid to grow, and mixes sowing time ripe;
Second Year positive season plantation F2, selects the good sterile strain of Comprehensive Traits to cut blue or green regeneration, selects 20 seeds regenerating individual plant time ripe during heading; Winter in the same year Hainan plantation F3, time ripe, totally 20 strain fecundities are good in choosing, and strain Leaf pattern is good, the single-strain seed that Comprehensive Traits is excellent;
3rd year positive season plantation F4, the strain microscopy screening that the thorough strain Leaf pattern of abortion is good is selected during heading, the excellent sterile strain of Comprehensive Traits is selected to raise extensive 527 test crosses in restorer another name for Sichuan Province of rice No. 6 and the seed selection of paddy rice institute of Sichuan Agricultural University with the restorer of the lane housing institute of agricultural sciences of Jiangsu Province seed selection respectively, all the other cut blue or green regeneration and shading treatment is reserved seed for planting, screen through Identification of Comprehensive Characters, retain the sterile strain of 4 Comprehensive Traits excellences, and select this 4 strain regeneration single-strain seeds respectively; F5 is for selected 4 strains and test cross kind thereof for Hainan plantation of winter in the same year, in 4 sterile strains, selects general performance to give prominence to strain HS602 and test cross offspring thereof, selects excellent sterile individual plant further;
Within 4th year, plantation F6 is for the sterile individual plant of selected excellence, respectively again with restorer R5549, raise extensive 527 test crosses of rice No. 6 and another name for Sichuan Province, obtain test cross kind HS602/R5549, HS602/ and raise rice No. 6, HS602/ another name for Sichuan Province extensive 527, retain overall strain abortion thoroughly without the strain of pollen, cut blue or green regeneration and shading treatment is reserved seed for planting respectively; Winter in the same year Hainan plantation F7 raises rice No. 6, extensive 527 qualifications in HS602/ another name for Sichuan Province for strain selected by HS602 and test cross kind HS602/R5549, HS602/, to the strain of HS602 menu, and roguing system seed.
3. the selection of a kind of new rice variety according to claim 1, is characterized in that: restorer R5549 seed selection is specially:
First Year does female parent with China extensive 118, and osmanthus 99-2 makes paternal hybrid, winter in the same year Hainan plantation F1, plant and leaf shape is good, grow that delicate and pretty, solid normal mature seed is mixed to be received;
Second Year positive season plantation F2, colony's performance point from, select 7 leaf sheaths and seed thin membrane inside rush stalk point to be separate targets individual plant winter in the same year Hainan plantation F3 of purple;
10 target individual plants that 3rd year spring selected in the strain of Hainan 205 that Comprehensive Traits is good, leaf sheath and seed thin membrane inside rush stalk point are purple, positive season plantation in the same year F4 eliminates a strain in 10 strains, the menu strain respectively of residue strain, totally 9 individual plants;
Hainan, 4th year spring Hainan plantation F5 is for selected 9 individual plants, and positive season plantation in the same year F6, selects Comprehensive Traits to stablize, show excellent target strain 5; In Hainan breeding F7 generation winter in the same year, continue to select Comprehensive Traits to show excellent individual plant respectively in above-mentioned 5 strains;
Within 5th year, positive season breeding individual plant F8 shows excellent individual plant for above-mentioned 5 strain Comprehensive Traits, and be measured with II-32A, Xieqingzao A and Peiai 64S respectively, Hainan breeding of winter in the same year, and qualification is measured advantage, obtain strain 5549 combos conjunction ripening rates high, high yield and high quality, strain 5549 i.e. restorer R5549.
4. the selection of a kind of new rice variety according to claim 1, it is characterized in that: seed production way is specially: Parent was according to sowing gap stage 41 days, maternal 602S leaf number of the main culm 14.5 leaf, male parent R5549 leaf number of the main culm 18.5 leaf, the wide 210cm in railway carriage or compartment, male parent 1 row, the capable plantation of maternal 10-12, meet when ensureing Parent florescence flower in the safe fertility alteration period of maternal 602S, carry out 602S/R5549 hybrid seeding.
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Cited By (5)
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| CN105660386A (en) * | 2016-03-29 | 2016-06-15 | 浙江省嘉兴市农业科学研究院（所） | Breeding method of conventional early Indica rice |
| CN106577262A (en) * | 2016-12-16 | 2017-04-26 | 湖南杂交水稻研究中心 | Breeding method for multi-ear super hybrid rice restorer line with high tillering capability |
| CN110506624A (en) * | 2019-09-24 | 2019-11-29 | 广西力拓粮油加工有限公司 | A kind of selection of rice varieties |
| CN111543314A (en) * | 2020-06-29 | 2020-08-18 | 四川发生种业有限责任公司 | Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line |
| CN115176699A (en) * | 2022-08-01 | 2022-10-14 | 湖南仕叶生态农业科技发展有限公司 | Rice cultivation technology |
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| CN111543314A (en) * | 2020-06-29 | 2020-08-18 | 四川发生种业有限责任公司 | Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line |
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Application publication date: 20151216 |