CN111543314A - Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line - Google Patents
Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line Download PDFInfo
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- CN111543314A CN111543314A CN202010605593.XA CN202010605593A CN111543314A CN 111543314 A CN111543314 A CN 111543314A CN 202010605593 A CN202010605593 A CN 202010605593A CN 111543314 A CN111543314 A CN 111543314A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 61
- 235000009566 rice Nutrition 0.000 title claims abstract description 61
- 238000009395 breeding Methods 0.000 title claims abstract description 22
- 201000010099 disease Diseases 0.000 title claims abstract description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 21
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 60
- 238000012360 testing method Methods 0.000 claims abstract description 40
- 238000003306 harvesting Methods 0.000 claims abstract description 21
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- 230000008901 benefit Effects 0.000 claims abstract description 18
- 208000035240 Disease Resistance Diseases 0.000 claims abstract description 15
- 238000012216 screening Methods 0.000 claims abstract description 14
- 238000013102 re-test Methods 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 9
- 230000001172 regenerating effect Effects 0.000 claims abstract description 5
- 230000035558 fertility Effects 0.000 claims description 14
- 230000009418 agronomic effect Effects 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000005520 cutting process Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 238000009331 sowing Methods 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 2
- 230000008929 regeneration Effects 0.000 claims description 2
- 238000011069 regeneration method Methods 0.000 claims description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 230000002068 genetic effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention provides a breeding method of a photo-thermo-sensitive genic male sterile line of rice with high quality, high yield and disease resistance, which is characterized in that the photo-thermo-sensitive genic male sterile line is used as a female parent in summer to be hybridized with high-quality, high-yield and disease-resistant two-line hybrid rice to obtain a compound cross F1 generation; selecting single plants transformed to sterile in the early normal maturing later period to harvest seeds in spring of the second year in winter to obtain F2 generation, screening rice quality, planting in summer, and harvesting good single plants to obtain F3 generation; planting F3 in winter to obtain F4 generations; test crossing the summer seed F4 with the restoring line to obtain test crossing F1 generation, regenerating and harvesting seed to obtain F5 generation, and screening test crossing F1 generation rice quality; carrying out summer planting test cross F1 generation dominance identification in the fourth year; re-testing F5 generations corresponding to the strong dominance combination in winter, and breeding by adding generations to obtain re-tested F1 generations and F6 generations; re-testing the advantages of F1 in summer in the fifth year, and planting F6 generations to obtain F7 generations; the strong-dominance combination planted in winter is obtained by corresponding to F7 generation. The present invention relates to a new photo-thermo sensitive genic male sterile line which is bred by using high-quality, high-yield and disease-resistant two-line hybrid rice variety as male parent and hybridizing with photo-thermo sensitive genic male sterile line.
Description
Technical Field
The invention belongs to the technical field of rice seed selection, and particularly relates to a breeding method of a high-quality, high-yield and disease-resistant photo-thermo-sensitive genic male sterile line of rice.
Background
Disease resistance, yield and quality of rice have become the main targets of current rice breeding. The disease resistance of a plurality of high-quality photo-thermo-sensitive genic male sterile lines utilized in the current production is general, the rice blast resistance of the new variety of the two-line hybrid rice is improved and benefits from the resistance of a male parent, and a breeder cannot easily introduce the sterile line to protect own intellectual property right, while the high-quality high-yield disease-resistant two-line hybrid rice sold in the market is easier to introduce and has better rice blast resistance.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a breeding method of a high-quality, high-yield and disease-resistant photo-thermo-sensitive genic male sterile line of rice, aiming at the defects of the prior art.
In order to solve the technical problems, the invention adopts the technical scheme that: a breeding method of a high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line comprises the following steps:
s1, in the first summer, in Jiangtou city, hybridizing a photo-thermo-sensitive genic male sterile line with low critical temperature of fertility conversion as a female parent with high-quality, high-yield and disease-resistant two-line hybrid rice to obtain a compound cross F1 generation;
s2, planting compound cross F1 generation obtained in S1 in Hainan in winter of the current year, and selecting a single plant which has excellent agronomic characters, disease resistance and is transformed into sterile plants in the early and later fruiting period in spring of the second year to harvest seeds to obtain F2 generation;
s3, screening rice quality of the F2 generation obtained in the S2, planting in the oil city of the river in summer of the next year, re-identifying the field fertility and resistance, and selecting excellent single-plant storage rice piles in the strains with excellent agronomic characters, low disease resistance and low fertility conversion critical temperature in the last 8 months to regenerate, harden and harvest to obtain the F3 generation;
s4, planting the F3 generation obtained in S3 in the south of Hainan province in the winter of the next year, and selecting a single plant with excellent agronomic characters to harvest seeds to obtain an F4 generation;
s5, planting F4 generations in oil city in summer in the third year, performing test crossing by using a high-combining-ability high-quality restoring line, sowing the high-combining-ability high-quality restoring line in 3 periods to enable the high-combining-ability high-quality restoring line to meet the flowering period of the F4 generations, planting the high-combining-ability high-quality restoring line in an inter-compartment passageway of the F4 generations, isolating the fields of the F4 generations from other rice, removing flowers and pollinating to obtain a test crossing F1 generation, harvesting test crossing F1 generations, storing rice piles for regeneration, harvesting seeds to obtain an F5 generation, roughing the obtained test crossing F1 generation, screening rice quality, and eliminating the test crossing F1 generation with poor rice quality and strains of the F5 generation corresponding to the eliminated test crossing F1 generation;
s6, planting the screened test cross F1 generation of rice quality obtained in S5 in oil city in summer of the fourth year, and performing advantage identification;
s7, screening out a strong dominant combination in the testing cross F1 generation in S5 according to the testing result of the dominant identification of the testing cross F1 generation in S6, taking the F5 generation in S5 corresponding to the strong dominant combination in the testing cross F1 generation as a female parent in Hainan in the fourth year in winter, and performing retest by using a high-combining-ability high-quality recovery system to obtain a retest F1 generation; simultaneously, carrying out generation-added propagation on F5 generations corresponding to the strong dominant combination to obtain F6 generations;
s8, re-testing the F1 generation obtained in the oil planting S7 in summer of the fifth year, and performing advantage re-identification; planting F6 generation obtained in S7, cutting pile, regenerating and harvesting to obtain F7 generation;
s9, screening out strong advantage combinations in the retested F1 generation according to the test result of retesting the advantages of the F1 generation in the S8, planting F7 generation in S8 corresponding to the strong advantage combinations in the retested F1 generation in Hainan in winter in the fifth year to obtain F8 generation, and obtaining the high-quality high-yield disease-resistant rice photo-thermo-sensitive genic male sterile line with low fertility conversion critical temperature.
Preferably, the photo-thermo genic male sterile line with low fertility conversion critical temperature in the S1 is the photo-thermo genic male sterile line deep 08S.
Preferably, the high-quality, high-yield and disease-resistant two-line hybrid rice in S1 is the two-line hybrid rice Y-Liangyou No. 1.
Preferably, the number of the plants in the S2 of the compound cross F1 generation is more than or equal to 1000.
Preferably, the agronomic traits of S2-S4 are growth period, leaf morphology, plant height and stigma exposure rate.
Preferably, the high-combining-ability high-quality restorer lines of S5 and S7 are restorer line Wushan silk seedlings.
Preferably, the methods of dominance identification in S6 and dominance re-identification in S8 are both yield and resistance identification.
Compared with the prior art, the invention has the following advantages:
the invention utilizes the high-quality high-yield disease-resistant two-line hybrid rice variety as the male parent to hybridize with the photo-thermo-sensitive genic male sterile line to breed the new photo-thermo-sensitive genic male sterile line with high quality, high yield and rice blast resistance, breaks the blockade of breeders on breeding materials to a certain extent, expands the genetic basis of the breeding materials, improves the breeding efficiency, is expected to breed the photo-thermo-sensitive genic male sterile line with good resistance and high quality, and further prepares a new high-quality disease-resistant high-yield rice variety.
The present invention will be described in further detail with reference to examples.
Detailed Description
Example 1
The embodiment provides a breeding method of a high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line, which comprises the following steps:
s1, hybridizing a photo-thermo-sensitive genic male sterile line deep 08S with low critical temperature of fertility conversion as a female parent with a high-quality, high-yield and disease-resistant two-line hybrid rice Y-Eryou No. 1 in Jiangzou city in summer in the first year to obtain a compound cross F1 generation;
s2, planting compound cross F1 generation obtained in S1 in Hainan in winter of the current year, and selecting a single plant which has excellent agronomic characters, disease resistance and is transformed into sterile plants in the early and later fruiting period in spring of the second year to harvest seeds to obtain F2 generation; the number of the plants of the compound cross F1 generation is more than or equal to 1000;
s3, screening rice quality of the F2 generation obtained in the S2, planting in the oil city of the river in summer of the next year, re-identifying the field fertility and resistance, and selecting excellent single-plant storage rice piles in the strains with excellent agronomic characters, low disease resistance and low fertility conversion critical temperature in the last 8 months to regenerate, harden and harvest to obtain the F3 generation;
s4, planting the F3 generation obtained in S3 in the south of Hainan province in the winter of the next year, and selecting a single plant with excellent agronomic characters to harvest seeds to obtain an F4 generation;
s5, planting F4 generations in oil city in summer in the third year, performing test crossing on the high-combining-force high-quality restorer line Wushan silk seedlings, sowing the high-combining-force high-quality restorer line Wushan silk seedlings in 3 stages to enable the high-combining-force high-quality restorer line Wushan silk seedlings to meet the F4 generation flower periods, planting the high-combining-force high-quality restorer line Wushan silk seedlings in the aisles among the F4 generations, isolating the F4 generation field from other rice, driving flowers and pollinating to obtain a test crossing F1 generation, harvesting test crossing F1 generation, storing rice stakes, regenerating and harvesting seeds to obtain an F5 generation, generating rough test crossing F1 generation, performing rice quality screening, eliminating test crossing F1 generation with poor rice quality and strain strains of F5 generation corresponding to the eliminated test crossing F1 generation;
s6, planting the screened test cross F1 generation of rice quality obtained in S5 in oil city in summer of the fourth year, and performing advantage identification;
s7, screening out a strong dominant combination in the testing cross F1 generation in S5 according to the testing result of the dominant identification of the testing cross F1 generation in S6, taking the F5 generation in S5 generation corresponding to the strong dominant combination in the testing cross F1 generation as a female parent in Hainan in the fourth year in winter, and performing retest on the female parent by using a high-combining-ability high-quality restorer line Wushan Si Miao to obtain retest F1 generation; simultaneously, carrying out generation-added propagation on F5 generations corresponding to the strong dominant combination to obtain F6 generations;
s8, re-testing the F1 generation obtained in the oil planting S7 in summer of the fifth year, and performing advantage re-identification; planting F6 generation obtained in S7, cutting pile, regenerating and harvesting to obtain F7 generation;
s9, screening out a strong advantage combination in the retested F1 generation according to the re-identified test result of the advantages of the retested F1 generation in S8, planting the F7 generation in S8 corresponding to the strong advantage combination in the retested F1 generation in Hainan in winter in the fifth year to obtain an F8 generation, and obtaining a high-quality high-yield disease-resistant rice photo-thermo-sensitive genic male sterile line with low fertility conversion critical temperature;
in the embodiment, the agronomic characters from S2 to S4 are growth period, plant leaf form, plant height and stigma exposure rate; the methods of superiority identification in S6 and superiority re-identification in S8 were both yield and resistance identification.
In the embodiment, the new photo-thermo sensitive genic male sterile line with high quality, high yield and rice blast resistance is bred by hybridizing the photo-thermo sensitive genic male sterile line and the high-quality, high-yield and disease-resistant two-line hybrid rice variety serving as a male parent, so that the blockade of breeders on breeding materials is broken to a certain extent, the genetic basis of the breeding materials is expanded, and the breeding efficiency is improved.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way. Any simple modification, change and equivalent changes of the above embodiments according to the technical essence of the invention are still within the protection scope of the technical solution of the invention.
Claims (7)
1. A breeding method of a high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line is characterized by comprising the following steps:
s1, in the first summer, in Jiangtou city, hybridizing a photo-thermo-sensitive genic male sterile line with low critical temperature of fertility conversion as a female parent with high-quality, high-yield and disease-resistant two-line hybrid rice to obtain a compound cross F1 generation;
s2, planting compound cross F1 generation obtained in S1 in Hainan in winter of the current year, and selecting a single plant which has excellent agronomic characters, disease resistance and is transformed into sterile plants in the early and later fruiting period in spring of the second year to harvest seeds to obtain F2 generation;
s3, screening rice quality of the F2 generation obtained in the S2, planting in the oil city of the river in summer of the next year, re-identifying the field fertility and resistance, and selecting excellent single-plant storage rice piles in the strains with excellent agronomic characters, low disease resistance and low fertility conversion critical temperature in the last 8 months to regenerate, harden and harvest to obtain the F3 generation;
s4, planting the F3 generation obtained in S3 in the south of Hainan province in the winter of the next year, and selecting a single plant with excellent agronomic characters to harvest seeds to obtain an F4 generation;
s5, planting F4 generations in oil city in summer in the third year, performing test crossing by using a high-combining-ability high-quality restoring line, sowing the high-combining-ability high-quality restoring line in 3 periods to enable the high-combining-ability high-quality restoring line to meet the flowering period of the F4 generations, planting the high-combining-ability high-quality restoring line in an inter-compartment passageway of the F4 generations, isolating the fields of the F4 generations from other rice, removing flowers and pollinating to obtain a test crossing F1 generation, harvesting test crossing F1 generations, storing rice piles for regeneration, harvesting seeds to obtain an F5 generation, roughing the obtained test crossing F1 generation, screening rice quality, and eliminating the test crossing F1 generation with poor rice quality and strains of the F5 generation corresponding to the eliminated test crossing F1 generation;
s6, planting the screened test cross F1 generation of rice quality obtained in S5 in oil city in summer of the fourth year, and performing advantage identification;
s7, screening out a strong dominant combination in the testing cross F1 generation in S5 according to the testing result of the dominant identification of the testing cross F1 generation in S6, taking the F5 generation in S5 corresponding to the strong dominant combination in the testing cross F1 generation as a female parent in Hainan in the fourth year in winter, and performing retest by using a high-combining-ability high-quality recovery system to obtain a retest F1 generation; simultaneously, carrying out generation-added propagation on F5 generations corresponding to the strong dominant combination to obtain F6 generations;
s8, re-testing the F1 generation obtained in the oil planting S7 in summer of the fifth year, and performing advantage re-identification; planting F6 generation obtained in S7, cutting pile, regenerating and harvesting to obtain F7 generation;
s9, screening out strong advantage combinations in the retested F1 generation according to the test result of retesting the advantages of the F1 generation in the S8, planting F7 generation in S8 corresponding to the strong advantage combinations in the retested F1 generation in Hainan in winter in the fifth year to obtain F8 generation, and obtaining the high-quality high-yield disease-resistant rice photo-thermo-sensitive genic male sterile line with low fertility conversion critical temperature.
2. The breeding method of the photo-thermo genic male sterile line of rice with good quality, high yield and disease resistance as claimed in claim 1, wherein the photo-thermo genic male sterile line with low fertility conversion critical temperature in S1 is photo-thermo genic male sterile line deep 08S.
3. The breeding method of the photo-thermo sensitive genic male sterile line of rice with good quality, high yield and disease resistance as claimed in claim 1, wherein the high-quality, high-yield and disease-resistant two-line hybrid rice in S1 is two-line hybrid rice Y-Liangyou No. 1.
4. The breeding method of the photo-thermo sensitive genic male sterile line of rice with good quality, high yield and disease resistance as claimed in claim 1, wherein the number of the plants of the multiple crossing F1 generation in S2 is more than or equal to 1000.
5. The breeding method of the photo-thermo sensitive genic male sterile line of rice with good quality, high yield and disease resistance as claimed in claim 1, wherein the agronomic traits of S2-S4 are growth period, leaf morphology, plant height and stigma exsertion rate.
6. The breeding method of the photo-thermo sensitive genic male sterile line of rice with high quality, high yield and disease resistance as claimed in claim 1, wherein the high combining ability high quality restorer lines of S5 and S7 are all restorer line Wushan silk seedlings.
7. The breeding method of the photo-thermo sensitive genic male sterile line of rice with good quality, high yield and disease resistance as claimed in claim 1, wherein the methods of dominant determination in S6 and dominant re-determination in S8 are both yield and resistance determination.
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| CN112640776A (en) * | 2021-02-20 | 2021-04-13 | 湖南袁创超级稻技术有限公司 | Breeding of rice photo-thermo sensitive sterile line with high outcrossing rate and seed production method thereof |
| CN112704006A (en) * | 2021-02-23 | 2021-04-27 | 四川发生种业有限责任公司 | Breeding method of high-quality high-combining-ability indica-japonica hybrid biased japonica restorer |
| CN112913684A (en) * | 2021-03-19 | 2021-06-08 | 四川发生种业有限责任公司 | Breeding method of high-quality indica-japonica rice cross biased indica rice restorer line with high combining ability |
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| CN112704006A (en) * | 2021-02-23 | 2021-04-27 | 四川发生种业有限责任公司 | Breeding method of high-quality high-combining-ability indica-japonica hybrid biased japonica restorer |
| CN112913684A (en) * | 2021-03-19 | 2021-06-08 | 四川发生种业有限责任公司 | Breeding method of high-quality indica-japonica rice cross biased indica rice restorer line with high combining ability |
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Application publication date: 20200818 |