CN108207614A - A kind of precocity type cotton breeding new method - Google Patents

A kind of precocity type cotton breeding new method Download PDF

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Publication number
CN108207614A
CN108207614A CN201711482305.0A CN201711482305A CN108207614A CN 108207614 A CN108207614 A CN 108207614A CN 201711482305 A CN201711482305 A CN 201711482305A CN 108207614 A CN108207614 A CN 108207614A
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China
Prior art keywords
generation
cotton
breeding
precocity
precocious
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CN201711482305.0A
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Inventor
王青连
张金宝
董娜
张新
付远志
孙润润
王园园
李成奇
胡根海
张志勇
秦腾飞
薛惠云
张晓红
韩振甫
谭阳光
董涛
韦春艳
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Henan Institute of Science and Technology
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Henan Institute of Science and Technology
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Priority to CN201711482305.0A priority Critical patent/CN108207614A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention belongs to variety of crops breeding technique field, more particularly to a kind of precocious type cotton breeding new method, including cotton material ripe in as female parent, Gossypium klotzschianumAnderss is male parent, is hybridized, and obtains F1Generation, with F1On behalf of female parent, Gossypium klotzschianumAnderss is male parent, is returned, and obtains F2Generation, by F2It is selfed for south numerous added-generation, obtains F3Generation, with F3On behalf of male parent, in ripe cotton material be female parent, be returned, obtain F4Generation, mostly generation backcrossing, screening obtain meeting the precocity of selection and breeding standard, high yield FnIn generation, is repeatedly selfed, until precocious, high yield struture is stablized.The present invention combines the merit of Gossypium wild species and cultigen, obtains the new cotton variety of precocious high yield, and agronomic shape is stablized by hybridizing, being returned and being selfed a variety of breeding modes, is suitble to Mechaniaed harvest, saves a large amount of costs of labor.

Description

A kind of precocity type cotton breeding new method
Technical field
The invention belongs to variety of crops breeding technique field, more particularly to a kind of precocious type cotton breeding new method.
Background technology
Cotton is Malvaceae (Malvaceae) Gossypium (Gossypium), originates in subtropical zone, there is Asiatic cotton, cotton, land Four Shoot apex of ground cotton and sea island cotton, the cotton cultivated in the world is mainly upland cotton and sea island cotton.The kind of China upland cotton It plants area and accounts for more than the 98% of the national cotton field gross area, there is the plantation of sea island cotton in Some Regions, Xinjiang.China is divided into three big main products Cotton region, including the Yangtze river basin, Yellow River basin and Northwest inland cotton region, with the development of modern agriculture, Cotton Production is to precocious, excellent Matter, high yield and further urgent suitable for the demand of the new varieties of mechanized harvesting.For cotton breeding, prematureness is even more one Important economical character, kind breeding time suitable for whether, directly affect utilization and increasing of the kind to given area light temperature resource Produce potentiality.The prematureness of cotton is the quantitative character by controlled by multiple genes.Kind is shortened by breeding method and biological method Breeding time, its prematureness of genetic improvement can improve the multiple crop index in Yangtze and Yellow rivers basin wheat cotton or oily two ripe cotton region of cotton, in nothing Frost season short Xinjiang Northern Cotton Regions can make full use of sunlight-heat reaources, and fertility cold spell in later spring early period is avoided to cause chilling injury and late growth stage Early frost endangers, so as to ensure output of cotton and quality.
But the breeding difficulty of this multiple target character is more, lacks the germ plasm resource for having both these characters at present first;Its Secondary is that existing Shine Early cotton this precocious source, hereditary basis wide in variety from king cotton are narrow;And between prematureness and yield, quality Negative correlation more exacerbate the difficulty of breeding, selection and breeding breakthrough varieties, it is necessary to have breakthrough material.Gossypium wild species have Many merits that cultigen is lacked, character variation range is big, is the important germ plasm resource of breeding.California, United States Xia Fute experiment stations are bred as the AcalaSJ system kinds of ATH types, fiber using Gossypium wild species plucked instrument Bai Shi cottons (G.thurberi) Quality is good, while resisting verticillium.South Carolina PeeDee agricultural experiment stations are hybridized using homotype and cutcross.Make Se Bai The fibre strength of family name cotton landwards gradually ooze by cotton, has broken hereditary negative correlation, is bred as the excellent PD system kinds of fiber quality.These Material not only becomes the important materials of high yield high-quality breeding in the U.S., but also is utilized extensively by other many countries.Cause This, the present invention utilizes the favorable genes of Gossypium wild species, selects a kind of precocious type cotton breeding new method.
Invention content
The present invention combines the merit of Gossypium wild species and cultigen, by hybridizing, being returned and being selfed a variety of breeding sides Formula obtains the new cotton variety of precocious high yield, and agronomic shape is stablized, and is suitble to Mechaniaed harvest, saves a large amount of costs of labor.
The present invention provides a kind of precocious type cotton breeding new methods, include the following steps:
S1, for ripe cotton material as female parent, Gossypium klotzschianumAnderss is male parent, is hybridized in, obtains F1Generation;
S2, with F1On behalf of female parent, Gossypium klotzschianumAnderss is male parent, is returned, and obtains F2Generation, by F2It is selfed for south numerous added-generation, Obtain F3Generation;
S3, with F in S23On behalf of male parent, in ripe cotton material be female parent, be returned, obtain F4Generation, from the F4Dai Zhong Selection is relatively precocious compared with it is maternal, and the more healthy and stronger individual of nutrient growth bears seeds selected F compared with its male parent5 Generation;Continue the ripe cotton material in and carry out mostly generation backcrossing as female parent, every time using relatively maternal precocious, opposite male parent stalwartness as original The next generation is then screened, until screening obtains precocity, the high yield F for meeting selection and breeding standardnGeneration;
S4, by the precocity screened in S3, high yield FnIn generation, postpones date of seeding plantation, increases Earliness selection pressure, carries out Selfing is therefrom screened relatively precocious, high yield strain and is born seeds as the next generation, the above-mentioned selfing step of repetition, until being met The strain that the precocity of selection and breeding standard, high yield struture are stablized, selection and breeding are into precocious type new cotton variety.
Preferably, in S1 in ripe cotton female parent material be nakamise 12.
Preferably, volumetric concentration is applied when hybridizing in S1 in maternal column cap as 20% honey solution, drop inside and outside bract The 30ppm basic elements of cell division.
Preferably, the breeding time of ripe cotton material is 130-140d in described.
Preferably, S4 mid-early maturities type new cotton variety boll per plant 12-15.
Compared with prior art, beneficial effects of the present invention:
It is male parent, and be returned twice that the present invention, which selects Gossypium wild species Gossypium klotzschianumAnderss, it is ensured that intake Gossypium wild species Inhereditary material, expands the hereditary basis of Mature breeding, at the same using nutrient growth is abundant, strain is healthy and strong, it is disease-resistant in ripe cotton Floral material nakamise 12 is female parent, and the precocious type with reference to the two merit is filtered out by hybridizing, being returned a variety of breeding modes Strain recycles the good recombinant of the fixed early maturing and high yield of selfing, stablizes agronomic shape, and selection and breeding obtain the cotton of precocious high yield New varieties are suitble to Mechaniaed harvest, save a large amount of costs of labor.
Specific embodiment
1 specific embodiment of the present invention is described in detail below, it is to be understood that protection scope of the present invention It is not restricted by specific implementation, the experimental method used in embodiment is conventional method unless otherwise specified, real Material used in example is applied, is commercially available.
Nakamise 12:It is recorded in " selection and breeding of nakamise 12 and its kind Journal of Sex Research Tan Lian prestiges, the positive moral Chinese agricultures section of Liu It learns, 1990.23 (3):A 12-19 " texts, the public can obtain at applicant, can only be used to repeat present invention experiment use.
Gossypium klotzschianumAnderss:It is recorded in that " landwards cotton gradually oozes Shen Xinlian, Zhu for Gossypium wild species Gossypium klotzschianumAnderss genome It is quiet, Zhang Xianggui, Zhang Baolong, Cao Zhibin, Yang Yuwen, Xu Peng, Xu Yingjun, ten thousand tide cotton journals of Ni, 2008,20 (4):256-263” One text, the public can obtain at applicant, can only be used to repeat present invention experiment use.
Grind cotton No. 21 in Shandong:It is recorded in " Upland Cotton contrast test Li Haiqi, Zhang Yulian agriculturals and technology 2013 (04), in ", the public can obtain at applicant, can only be used to repeat present invention experiment use.
Embodiment 1
A kind of precocity type cotton breeding new method, includes the following steps:
S1, autumn sowing kind, using breeding time as 140d or so in ripe cotton material nakamise 12 be maternal, Gossypium klotzschianumAnderss is Male parent, parent use potting, and winter is placed in greenhouse, and emasculation before female parent is bloomed is hybridized, and applies body in maternal column cap during hybridization The honey solution of product a concentration of 20%, the drop 30ppm basic elements of cell division inside and outside bract, continuous 4-7d 1 time a day, adjust fertilization Process and embryonic development, spring in the coming year obtain hybridization F1Generation;
S2, with F1On behalf of female parent, Gossypium klotzschianumAnderss is male parent, and maternal emasculation is returned, and obtains F2Generation, by F2Dai Nanfan Generation is added to be selfed, obtains F3Generation;
S3, with F in S23On behalf of male parent, in ripe cotton material nakamise 12 be female parent, be returned, obtain F4Generation, from institute State F4In generation selection with its female parent compared to relatively it is precocious (breeding time 120-130d, bloom knot bell faster, blow-of-cottons are smooth and concentration), and The individual of nutrient growth more healthy and stronger (plant height 90-110cm, boll per plant 10-15, well developed root system) is tied compared with its male parent Seed is selected in F5Generation;Continue the ripe cotton material nakamise 12 in and carry out the backcrossing of 7 generations to be maternal, every time with relatively maternal early Ripe, opposite male parent stalwartness is next-generation for principle screening, and until screening obtains meeting selection and breeding standard, breeding time is the morning of 100-110d Ripe, high yield (refers to compared with same type check variety, more than 10% per mu yield gined cotton volume increase) F12Generation;
S4, by the precocity screened in S3, high yield F12In generation, postpones date of seeding plantation, and late June plantation increases prematureness choosing Select pressure, late August topping, set isolation pocket is selfed, and it is next therefrom to screen that relatively precocious, high yield strain is born seeds In generation, repeats above-mentioned selfing step, until F16In generation, obtains meeting selection and breeding standard, and breeding time is the precocity of 100d or so, boll per plant The strain that 12-15, high yield struture are stablized, selection and breeding are into precocious type new cotton variety.
Economical character for the precocious type new cotton variety for verifying selection and breeding of the present invention, carries out tests below scheme:
Test variety:Kind of the present invention compares and grinds cotton No. 21 for Shandong, using method of comparison, selects 5 local fields respectively Experiment, it is 1 mu that area is planted experimentally in each place, is divided into two groups, using consistent cultural method.
Sowing and density:Live streaming in 20 days or so May, 0.8 meter of line-spacing, 0.4 meter of spacing in the rows.
It applies fertilizers scientifically:Often apply common farm manure 1800Kg/ mus of market, 16Kg/ mus of urea, 38Kg/ mus of phosphate fertilizer, potash fertilizer 18Kg/ mus, 3Kg/ mus of boron fertilizer makees base fertilizer, is applied before sowing once, seedling flower bud phase suitably increases 3Kg/ mus of urea according to growing way, hence it is evident that Zinc-deficiency, the field foliage-spray zinc sulfate of boron trace element, boric acid solution.
Observation is recorded:The breeding time of kind, Bolls per plant, lint yield.It the results are shown in Table 1.
Table 1 respectively tests the indices average of regional statistics
By result in table it is found that the precocious type new cotton variety breeding time of selection and breeding of the present invention is in 100d or so, meet existing Precocious type cotton seed standard, in addition kind Bolls per plant of the present invention is higher, and lint yield is high, has the economic valency promoted the use of Value.
It should be noted that the step method used in claims of the present invention is same as the previously described embodiments, in order to anti- Only repeat, description of the invention preferred embodiment, but those skilled in the art once know it is basic creative general It reads, then additional changes and modifications may be made to these embodiments.So appended claims are intended to be construed to include preferably in fact It applies example and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies Within, then the present invention is also intended to include these modifications and variations.

Claims (5)

1. a kind of precocity type cotton breeding new method, which is characterized in that include the following steps:
S1, for ripe cotton material as female parent, Gossypium klotzschianumAnderss is male parent, is hybridized in, obtains F1Generation;
S2, with F1On behalf of female parent, Gossypium klotzschianumAnderss is male parent, is returned, and obtains F2Generation, by F2It is selfed, obtains for south numerous added-generation F3Generation;
S3, with F in S23On behalf of male parent, in ripe cotton material be female parent, be returned, obtain F4Generation, from the F4It is selected in generation It is precocious compared with it is maternal, and the more healthy and stronger individual of nutrient growth bears seeds selected F compared with its male parent5Generation;Continue with In ripe cotton material carry out mostly generation backcrossing to be maternal, every time using relatively maternal precocious, opposite male parent stalwartness under principle screening A generation, until screening obtains precocity, the high yield F for meeting selection and breeding standardnGeneration;
S4, by the precocity screened in S3, high yield FnIn generation, postpones date of seeding plantation, increases Earliness selection pressure, is selfed, Relatively precocious, high yield strain is therefrom screened to bear seeds as the next generation, the above-mentioned selfing step of repetition, until obtaining meeting selection and breeding mark The strain that accurate precocity, high yield struture are stablized, selection and breeding are into precocious type new cotton variety.
2. precocity type cotton breeding new method as described in claim 1, which is characterized in that ripe cotton female parent material is in S1 Nakamise 12.
3. precocity type cotton breeding new method as described in claim 1, which is characterized in that applied in maternal column cap when hybridizing in S1 Volumetric concentration is 20% honey solution, the drop 30ppm basic elements of cell division inside and outside bract.
4. precocity type cotton breeding new method as described in claim 1, which is characterized in that the fertility of ripe cotton material in described Phase is 130-140d.
5. precocity type cotton breeding new method as described in claim 1, which is characterized in that S4 mid-early maturity type new cotton variety lists 12-15, strain knot bell.
CN201711482305.0A 2017-12-29 2017-12-29 A kind of precocity type cotton breeding new method Pending CN108207614A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042301A (en) * 2018-08-24 2018-12-21 新疆守信种业科技有限责任公司 A kind of new varieties HC-159 selection of no film cotton
CN109105252A (en) * 2018-08-25 2019-01-01 中国农业科学院棉花研究所 A kind of method of quickly breeding new cotton variety
CN109479660A (en) * 2018-12-04 2019-03-19 安徽省农业科学院棉花研究所 A kind of accurate breeding method of short season cotton new varieties

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1219347A (en) * 1997-12-12 1999-06-16 中国科学院遗传研究所 Distant hybridization and breeding method for cotton
CN1568667A (en) * 2004-04-27 2005-01-26 中国农业科学院棉花研究所 Distant hybridization breeding method for color cotton
CN105010129A (en) * 2015-08-05 2015-11-04 江苏省农业科学院 Precocity upland cotton new-germplasm breeding method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1219347A (en) * 1997-12-12 1999-06-16 中国科学院遗传研究所 Distant hybridization and breeding method for cotton
CN1568667A (en) * 2004-04-27 2005-01-26 中国农业科学院棉花研究所 Distant hybridization breeding method for color cotton
CN105010129A (en) * 2015-08-05 2015-11-04 江苏省农业科学院 Precocity upland cotton new-germplasm breeding method

Non-Patent Citations (3)

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Title
YUQIANG SUN等: "Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion", 《THEOR APPL GENET》 *
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042301A (en) * 2018-08-24 2018-12-21 新疆守信种业科技有限责任公司 A kind of new varieties HC-159 selection of no film cotton
CN109105252A (en) * 2018-08-25 2019-01-01 中国农业科学院棉花研究所 A kind of method of quickly breeding new cotton variety
CN109479660A (en) * 2018-12-04 2019-03-19 安徽省农业科学院棉花研究所 A kind of accurate breeding method of short season cotton new varieties

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