CN103348908A - Method for selecting hybrid seeds of Brassica juncea var. multiceps - Google Patents
Method for selecting hybrid seeds of Brassica juncea var. multiceps Download PDFInfo
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Abstract
The invention discloses a method for selecting the hybrid seeds of Brassica juncea var. multiceps. Yongxue No.3 of Brassica juncea var. multiceps is bred through hybrid seed production of Ningbo fine-leaf yellow Brassica juncea var. multiceps cytoplasm male sterile line 07-50A and fine-leaf mustard 07-2 inbred line Brassica juncea var. multiceps as a female parent and a male parent respectively. The Ningbo fine-leaf yellow Brassica juncea var. multiceps cytoplasm male sterile line 07-50A is obtained by hybridizing a cytoplasm male sterile source as a female parent and Ningbo fine-leaf yellow Brassica juncea var. multiceps to obtain an F1 generation and carrying out backcross breeding by using the Ningbo fine-leaf yellow Brassica juncea var. multiceps as a recurrent parent; and the male parent fine-leaf mustard 07-2 inbred line Brassica juncea var. multiceps is obtained by the inbred purification of a local variety of fine-leaf mustard having excellent comprehensive properties. The new species Yongxue No.3 obtained through the method has the advantages of high output, strong disease resistances, high stalk-leaf ratio, excellent processing property, and suitableness for the plantation of winter Brassica juncea var. multiceps.
Description
Technical field
The present invention relates to the vegetable breeding technical field, particularly relate to a kind of new potherb mustard crossbreed---the selection that No. 3, river in Zhejiang Province snow.
Background technology
Potherb mustard (Brassica juncea Coss.var.multiceps Tsen et Lee.) is commonly called as potherb mustard, belongs to the Cruciferae rape and belongs to the leaf mustard kind, is the mutation of mustard of tillering.Potherb mustard is extensively planted in China, is one of main pickless of China; Potherb mustard can repeatedly be planted in China in general 1 year, the dish that troats that the winter sowing spring receives, and the autumn sowing winter receives cries preserved vegetable; Because the salted vegetables quality that the winter snows dish is made is better, and can avoid the harmful effect of bolting in spring, winter snows dish area under cultivation is the trend that increases gradually.Potherb mustard germ plasm resource is abundant, and hybrid vigour is obvious, and the upper application of production is conventional variety substantially.Main breed is degenerated, and disease is on the rise, and yield and quality reduces, and these problems become the bottleneck that restricts China's potherb mustard secondary industry sustainable development, in the production in the urgent need to the update of potherb mustard kind.Utilizing hybrid vigour is the important channel of improving the potherb mustard yield and quality, and seed selection potherb mustard hybrid new breed for driving peasant's volume increase, increasing income, satisfies production and process requirements and has great importance; But there are Ogura, Pol, hua, OuxinA etc. in the cytoplasmic male sterility source of having reported the Hybrid breeding in commercial system utilization; Up to now, there is not yet the report of relevant potherb mustard hybrid seed breeding method.
Summary of the invention
The technical problem to be solved in the present invention provides that a kind of output is high, disease resistance is strong, stalk Ye Bigao, processing trait are good, the selection of the hybridization potherb mustard new varieties that suitable winter snows colza is planted.
The selection of a kind of potherb mustard crossbreed, take Ningbo spire yellow race potherb mustard (No. 18, Yin snow) cytoplasm male sterility line 07-50A as maternal, carry out hybrid seeding take spire leaf mustard 07-2 inbred line potherb mustard (No. 2, river in Zhejiang Province snow) as male parent, breed No. 3, potherb mustard river in Zhejiang Province snow.
The selection of potherb mustard crossbreed of the present invention, wherein said spire leaf mustard 07-2 inbred line potherb mustard are that the good landrace spire leaf mustard of Comprehensive Traits obtains by the selfing purifying.Used male parent 07-2 inbred line (spire leaf mustard) is the good landrace of Comprehensive Traits.The spire leaf mustard is Ningbo Local Excellent kind, and the leaf light green color is draped over one's shoulders pin, totally cleaves, and it is strong to have a growth potential, and field performance viral diseases, stalk leaf quality be than high, the advantage such as bright yellow color after the processing.Shortcoming is that stooling and bolting resistant property are medium, with male parent selfing purifying, is conducive to improve the purity of hybrid seeding.
The selection of potherb mustard crossbreed of the present invention, the transformation approach of the wherein said Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race is: take in the cytoplasmic male sterility source as the maternal and potherb mustard hybridization acquisition F of the Ningbo spire yellow race
1In generation, carry out again the continuous backcross transformation take Ningbo spire yellow race potherb mustard as recurrent parent and obtain cytoplasm male sterility line 07-50A.Described maternal 07-50A is that brassicaceous vegetable breeding seminar of Ningbo City Agriculture Science Academy becomes with the potherb mustard transformation of the landrace Ningbo spire yellow race, the cytoplasm male sterility line that Comprehensive Traits is good, its leaf green is draped over one's shoulders pin, drastic crack, have the output height, stooling is strong, virus resistance disease, anti-bolting, the advantages such as processing trait is good, shortcoming are that stalk leaf quality ratio has much room for improvement, and color of processing is partially black.After cytoplasmic male sterility source and the potherb mustard hybridization of the Ningbo spire yellow race, again take Ningbo spire yellow race potherb mustard as the recurrent parent continuous backcross, be actually the process of transformation cytoplasmic male sterile line 07-50A, the difference of 07-50A and Ningbo spire yellow race potherb mustard is, both economic characters are substantially the same, but 07-50A does not have pollen and stamen, performance male sterile.The potherb mustard transformation of the Ningbo spire yellow race is become cytoplasmic male sterile line 07-50A, can remove the work such as artificial emasculation in the hybrid seeding, pollination from, and can improve the purity of hybrid seed, provide great convenience for utilizing hybrid vigour seed selection first-filial generation new varieties.
The selection of potherb mustard crossbreed of the present invention, the cytoplasmic male sterility source of using in the wherein said Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race transformation approach is Ogura cytoplasmic male sterility source.Described sterile source sterility is thorough, and sterile plant rate and sterile degree all reach 100%.
The selection of potherb mustard crossbreed of the present invention comprises the steps:
(1) it is maternal selecting the Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race, and spire leaf mustard 07-2 inbred line potherb mustard is male parent;
(2) described male parent is in the late October outdoor sowing, and for making the Parent flower synchronization, the more described male parent of described female parent shifts to an earlier date sowing in 5-7 days;
(3) field planting in January in next year, Parent is planted by 1: 3 kind, distance between rows and hills 40cm * 27cm; Use sufficient base manure, every mu execute potassium sulphate type ternary composite fertilizer 50kg,, borax 1kg; Guarantee that 2000m is interior without other brassicaceous vegetables on every side; Early growth period is according to leaf, leaf look removal of impurities 2～3 times;
(4) on April, the middle ten days, the attention control diamond-back moth; During bolting, can insert the bamboo pole backguy and prevent the later stage lodging; The male parent initial bloom stage, bee pollination; The borax solution of florescence every mu of usefulness 0.5% and the KH of 0.2%-0.5%
2PO
4, evenly spray 2 times, make kind of pod full, improve seed production;
(5) mid-May, for preventing production of hybrid seeds old farmer, maternal seed generation mechanical admixture of planting in the strain, pull out the male parent row in the male parent flower end of term; Harvesting when early June, the kind pod was medium well obtains No. 3, potherb mustard river in Zhejiang Province snow.
The selection of potherb mustard crossbreed of the present invention wherein also comprises cultivation and the results of crossbreed afterwards in step (5), it specifically comprises the steps:
The crossbreed proper seeding of A results is preferably sowed in mid-August; Seeding row spacing 30cm * 35cm adjusts according to soil fertility, and the field plantation that fertility is high is rarer, and suitably plantation is closeer for the low field of fertility;
B needs the rich water amount large, uses sufficient base fertilizer, preferred every 667m
2With the mixture of superphosphate 40kg, carbonic hydroammonium 40kg and three kinds of materials of Nitrogen, Phosphorus and Potassium 40kg, the mixture of the muck 1000～1500kg that perhaps becomes thoroughly decomposed, two kinds of materials of Nitrogen, Phosphorus and Potassium 30～40kg is made base fertilizer; Optimum period Nitrogen Top Dressing; Keep the field moistening, anti-arid when short of rain, anti-ponding when rainy, it is supporting to carry out three ditches;
C carries out the control of the sick worms such as aphid, cabbage caterpillar, daicon leaf beetle, snail, virus disease, downy mildew, soft rot;
D gathers in the crops in good time, is preferable over around slight snow to gather.
The selection difference from prior art of potherb mustard crossbreed of the present invention is that it is maternal that selection of the present invention has been selected Ningbo spire yellow race potherb mustard (No. 18, Yin snow) cytoplasm male sterility line 07-50A, has the output height, stooling is strong, virus resistance disease, anti-bolting, the advantage such as processing trait is good; Spire leaf mustard 07-2 inbred line potherb mustard (river in Zhejiang Province snow No. 2) be male parent, and it is strong to have a growth potential, and field performance viral diseases, stalk leaf quality be than high, the advantages such as the rear bright yellow color of processing; The cytoplasmic male sterility source of using in the described maternal transformation approach is Ogura cytoplasmic male sterility source, and sterility is thorough, and sterile plant rate and sterile degree all reach 100%; Described selection has been specified high, the simple hybridization technique of a cover hybridization efficiency according to the characteristic of plant itself; New varieties output according to the method for the invention seed selection is high, disease resistance is strong, obstruct Ye Bigao, processing trait is good, and suitable winter snows colza is planted.
Be described further below in conjunction with the selection of accompanying drawing to potherb mustard crossbreed of the present invention.
Description of drawings
Fig. 1 is the family tree of the selection of potherb mustard crossbreed of the present invention.
Embodiment
As shown in Figure 1, the selection of potherb mustard crossbreed of the present invention comprises: take Ningbo spire yellow race potherb mustard (No. 18, Yin snow) cytoplasm male sterility line 07-50A as maternal, with 07-2 inbred line (spire leaf mustard, having another name called river in Zhejiang Province snow No. 2) potherb mustard is that male parent is carried out hybrid seeding, breeds new varieties---No. 3, potherb mustard river in Zhejiang Province snow.
Described female parent (the potherb mustard cytoplasm male sterility line 07-50A of the Ningbo spire yellow race) is that brassicaceous vegetable breeding seminar of Ningbo City Agriculture Science Academy becomes with the potherb mustard transformation of the landrace Ningbo spire yellow race, the cytoplasm male sterility line that Comprehensive Traits is good, its transformation approach is: take in the cytoplasmic male sterility source as the maternal and potherb mustard hybridization acquisition F of the Ningbo spire yellow race
1In generation, carry out again the continuous backcross transformation take Ningbo spire yellow race potherb mustard as recurrent parent and obtain cytoplasm male sterility line 07-50A.The cytoplasmic male sterility source of using in the described Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race transformation approach is Ogura cytoplasmic male sterility source.Described male parent (spire leaf mustard 07-2 inbred line potherb mustard) is that the good landrace spire leaf mustard of Comprehensive Traits obtains by the selfing purifying.
The selection of potherb mustard crossbreed of the present invention is specifically hybridized the cultivation process and is comprised the steps:
(1) it is maternal selecting the Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race, and spire leaf mustard 07-2 inbred line potherb mustard is male parent;
(2) described male parent is in the late October outdoor sowing, and for making the Parent flower synchronization, the more described male parent of described female parent shifts to an earlier date sowing in 5-7 days;
(3) field planting in January in next year, Parent is planted by 1: 3 kind, distance between rows and hills 40cm * 27cm; Use sufficient base manure, every mu execute potassium sulphate type ternary composite fertilizer 50kg,, borax 1kg; Guarantee that 2000m is interior without other brassicaceous vegetables on every side; Early growth period is according to leaf, leaf look removal of impurities 2～3 times;
(4) on April, the middle ten days, the attention control diamond-back moth; During bolting, can insert the bamboo pole backguy and prevent the later stage lodging; The male parent initial bloom stage, bee pollination; The borax solution of florescence every mu of usefulness 0.5% and the KH of 0.2%-0.5%
2PO
4, evenly spray 2 times, make kind of pod full, improve seed production;
(5) mid-May, for preventing production of hybrid seeds old farmer, maternal seed generation mechanical admixture of planting in the strain, pull out the male parent row in the male parent flower end of term; Harvesting when early June, the kind pod was medium well obtains No. 3 seeds of potherb mustard river in Zhejiang Province snow.
The selection of described potherb mustard crossbreed also comprises cultivation and the results of crossbreed afterwards in step (5), and it specifically comprises the steps:
The crossbreed of A results-No. 3 seed proper seedings of potherb mustard river in Zhejiang Province snow is preferably sowed in mid-August; Seeding row spacing 30cm * 35cm adjusts according to soil fertility, and the field plantation that fertility is high is rarer, and suitably plantation is closeer for the low field of fertility;
B needs the rich water amount large, uses sufficient base fertilizer, preferred every 667m
2With the mixture of superphosphate 40kg, carbonic hydroammonium 40kg and three kinds of materials of Nitrogen, Phosphorus and Potassium 40kg, the mixture of the muck 1000～1500kg that perhaps becomes thoroughly decomposed, two kinds of materials of Nitrogen, Phosphorus and Potassium 30～40kg is made base fertilizer; Keep the field moistening, anti-arid when short of rain, anti-ponding when rainy, it is supporting to carry out three ditches;
C carries out the control of the sick worms such as aphid, cabbage caterpillar, daicon leaf beetle, snail, virus disease, downy mildew, soft rot;
D gathers in the crops in good time, is preferable over around slight snow to gather.
No. 3 new varieties of river in Zhejiang Province snow that the present invention breeds are compared with the preferably Ningbo spire yellow race potherb mustard contrast of main Comprehensive Traits of planting, and have the following advantages:
(1) high yield
2010 and carried out the multiple spot varietal yield test on Yin state, Ningbo City, Ninghai, Cixi and other places autumn in 2011, No. 3 output of river in Zhejiang Province snow and contrast difference reached utmost point significance level (seeing Table 1).No. 3 per mu yield 5602.80～6698.35kg of autumn in 2010 river in Zhejiang Province snow, average 6283.14kg contrasts Ningbo spire yellow race potherb mustard and on average increases production 31.87%.No. 3 per mu yield 5869.60～6927.35kg of autumn in 2011 river in Zhejiang Province snow, average 6555.41kg, the average volume increase 33.98% of contrast.In 2 years multiple spot varietal yield tests, No. 3 per mu yield 6419.27kg of river in Zhejiang Province snow, the contrast volume increase 32.81%.
(2) disease-resistant
2011 to 2012; Zhejiang Academy of Agricultural Science plant protection and institute of microbiology have carried out viral diseases (TuMV) for No. 3 to potherb mustard new varieties river in Zhejiang Province snow and have identified and evaluation that test is carried out in Zhejiang Academy of Agricultural Science plant protection and institute of microbiology greenhouse.The investigation method of disease, Recording criteria are all undertaken by the unified standard that public relations seminar of country " eight or five " formulates, and check variety is Ningbo spire yellow race potherb mustard.Resistance Identification result (seeing Table 2) shows that river in Zhejiang Province snow shows as for No. 3 disease-resistant to virus disease (TuMV), and check variety is anti-in showing as.
(3) quality
River in Zhejiang Province No. 3 growth potentials of snow are strong, and stooling is strong, and stalk leaf quality ratio is high, the leaf light green color, and the outward appearance commodity is good, receives the welcome of planting base.Aspect processing quality, according to agricultural product quality and safety supervision and inspection center of the Ministry of Agriculture (Ningbo) testing result (seeing Table 3), No. 3 moisture of river in Zhejiang Province snow, protein content are a little less than contrast, content of reducing sugar contrasts increases by 5.26%, the crude fiber content contrast increases by 16.67%, and total ascorbic acid content contrasts increases by 8.33%, and calcium content contrasts increases by 6.80%, nitrate content contrasts low by 13.58%, is a colory leaf mustard kind.
No. 3, table 1 river in Zhejiang Province snow and contrast output are relatively
Annotate: units/kg/667m
2, same hurdle has different letter representation differences and reaches the 0.05(lowercase) and the 0.01(capitalization) significance level.
No. 3 Resistance Identification results of table 2 river in Zhejiang Province snow
Annotate: numeral is mean value in the table.
Table 3 river in Zhejiang Province snow is processed nutritional quality with contrast No. 3 and is compared
Above-described embodiment is described preferred embodiment of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (6)
1. the selection of a potherb mustard crossbreed is characterized in that: take the potherb mustard cytoplasm male sterility line 07-50A of the Ningbo spire yellow race as maternal, carry out hybrid seeding take spire leaf mustard 07-2 inbred line potherb mustard as male parent, breed No. 3, potherb mustard new varieties river in Zhejiang Province snow.
2. the selection of potherb mustard crossbreed according to claim 1, it is characterized in that: described spire leaf mustard 07-2 inbred line potherb mustard is that the good landrace spire leaf mustard of Comprehensive Traits obtains by the selfing purifying.
3. the selection of potherb mustard crossbreed according to claim 1 and 2, it is characterized in that: the transformation approach of the described Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race is: be maternally to obtain F with Ningbo spire yellow race potherb mustard hybridization take the cytoplasmic male sterility source
1In generation, carry out again the continuous backcross transformation take Ningbo spire yellow race potherb mustard as recurrent parent and obtain cytoplasm male sterility line 07-50A.
4. the selection of potherb mustard crossbreed according to claim 3, it is characterized in that: the cytoplasmic male sterility source of using in the described Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race transformation approach is Ogura cytoplasmic male sterility source.
5. the selection of potherb mustard crossbreed according to claim 4 is characterized in that: comprise the steps:
(1) it is maternal selecting the Ningbo potherb mustard cytoplasm male sterility line 07-50A of the spire yellow race, and spire leaf mustard 07-2 inbred line potherb mustard is male parent;
(2) described male parent is in the late October outdoor sowing, and for making the Parent flower synchronization, the more described male parent of described female parent shifts to an earlier date sowing in 5-7 days;
(3) field planting in January in next year, Parent is planted by 1: 3 kind, distance between rows and hills 40cm * 27cm; Use sufficient base manure, every mu execute potassium sulphate type ternary composite fertilizer 50kg,, borax 1kg; Guarantee that 2000m is interior without other brassicaceous vegetables on every side; Early growth period is according to leaf, leaf look removal of impurities 2～3 times;
(4) on April, the middle ten days, the attention control diamond-back moth; During bolting, can insert the bamboo pole backguy and prevent the later stage lodging; The male parent initial bloom stage, bee pollination; The borax solution of florescence every mu of usefulness 0.5% and the KH of 0.2%-0.5%
2PO
4, evenly spray 2 times, make kind of pod full, improve seed production;
(5) mid-May, for preventing production of hybrid seeds old farmer, maternal seed generation mechanical admixture of planting in the strain, pull out the male parent row in the male parent flower end of term; Harvesting when early June, the kind pod was medium well obtains No. 3 seeds of potherb mustard river in Zhejiang Province snow.
6. the selection of potherb mustard crossbreed according to claim 5 is characterized in that: also comprise afterwards cultivation and the results of crossbreed in step (5), it specifically comprises the steps:
The crossbreed of A results---No. 3 seed proper seedings of potherb mustard river in Zhejiang Province snow are preferably sowed in mid-August; Seeding row spacing 30cm * 35cm adjusts according to soil fertility, and the field plantation that fertility is high is rarer, and suitably plantation is closeer for the low field of fertility;
B needs the rich water amount large, uses sufficient base fertilizer, preferred every 667m
2With the mixture of superphosphate 40kg, carbonic hydroammonium 40kg and three kinds of materials of Nitrogen, Phosphorus and Potassium 40kg, the mixture of the muck 1000～1500kg that perhaps becomes thoroughly decomposed, two kinds of materials of Nitrogen, Phosphorus and Potassium 30～40kg is made base fertilizer; Optimum period Nitrogen Top Dressing; Keep the field moistening, anti-arid when short of rain, anti-ponding when rainy, it is supporting to carry out three ditches;
C carries out the control of the sick worms such as aphid, cabbage caterpillar, daicon leaf beetle, snail, virus disease, downy mildew, soft rot;
D gathers in the crops in good time, is preferable over around slight snow to gather.
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| CN104255441A (en) * | 2014-10-17 | 2015-01-07 | 宁波市农业科学研究院 | Method for breeding hybrid of spring tumorous stem mustard |
| CN106258935A (en) * | 2016-08-10 | 2017-01-04 | 宁波市农业科学研究院 | A kind of breeding method improving potherb mustard yield and processing quality |
| CN113229139A (en) * | 2021-06-03 | 2021-08-10 | 重庆市渝东南农业科学院 | High-yield seed production method for fresh-eating leaf mustard |
| CN114009336A (en) * | 2021-12-10 | 2022-02-08 | 宁波市农业科学研究院 | Breeding method for processing dried muslims type leaf mustard |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104255441A (en) * | 2014-10-17 | 2015-01-07 | 宁波市农业科学研究院 | Method for breeding hybrid of spring tumorous stem mustard |
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| CN106258935A (en) * | 2016-08-10 | 2017-01-04 | 宁波市农业科学研究院 | A kind of breeding method improving potherb mustard yield and processing quality |
| CN113229139A (en) * | 2021-06-03 | 2021-08-10 | 重庆市渝东南农业科学院 | High-yield seed production method for fresh-eating leaf mustard |
| CN114009336A (en) * | 2021-12-10 | 2022-02-08 | 宁波市农业科学研究院 | Breeding method for processing dried muslims type leaf mustard |
| CN116326476A (en) * | 2021-12-10 | 2023-06-27 | 宁波市农业科学研究院 | Breeding method of processed dried mustard type mustard |
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