KR20100137760A - A new interspecis and intergeneric f1 hybrid bred by using cellular male sterile line of baemoochae - Google Patents

Abstract

PURPOSE: A method for growing interspecies and intraspecies F1 hybrid plant using male sterile line of xBrassicoraphanus is provided to various F1 hybrid plants. CONSTITUTION: A method for growing interspecies and intraspecies F1 hybrid plant comprises: a step of inducing xBrassicoraphanus in a cytoplasmic male sterility(CMS); a step of growing fixed CMS; and a step of growing various F1 hybrid plant. The various F1 hybrid plant is F1 hybrid of xBrassicoraphanus CMS and Brassica campestris ssp., F1 hybrid of Brassica juncea CMS and xBrassicoraphanus, F1 hybride of Brassica juncea CMS and Brassica campestris ssp., and F1 hybrid of xBrassicoraphanus and Brassica juncea CMS.

Description

A new interspecis and intergeneric F1 hybrid bred by using cellular male sterile line of BaemooChae}

The present invention relates to a method of breeding intra- and interspecies 1 large hybrid plants using the male sterility line of Baemuchae.

The inventors of the present invention describe a hybrid plant of the genus "Birdweed," which is a hybrid between cabbage (Brassica campestris) and radish (Raphanus sativus).xBrassicoraphanus,genome: aarr, 2n = 38) Raparadish has been raised and patented (Patent No. 0492518, Patent name: New varieties of Baumduchae and its breeding method). After that, as a result of studying the agricultural characteristics of the plant, it was found that it can be widely used as raw materials of sprout vegetables, baby vegetables, ssam vegetables, yeolmu vegetables, kimchi vegetables and health supplements.

The baemu poles arbitrarily new plant was not the same world, so far as baechugwa botanical After a review of different plants and how hybrids are available in (十字花科, Cruciferae or Brassicaceae), the first ship to mobon radish and Chinese cabbage (Brassica rapa var. Pekinensis , genome: aa, 2n = 20), the hybridization was found to be very good. Second, the second genome rapeseed (Brasica napus , genome: aacc, 2n = 38), The cross hybridization was also found to be high in hybridization (Gard ( Brassica juncea , genome: aabb, 2n = 36)) and yellow mustard ( Brassica carinata , genome: bbcc, 2n = 34). Major characteristics improvement of vegetable crops and year-round production of assorted vegetables ", Table 9-13, 2006.7, Final Report, pages 28-31.

Therefore, if you are looking for a way to prevent the self-correction of a debt, for example, if there is a method of self-incompatibility or male infertility that does not allow self-correction and only corrects the other, the cross-bridging within the debt bond, as well as cross-border debt Martian species. Genus one genus hybrid (F1) plants were judged to be able to grow.

However, even if there are no self-incompatibilitys, such as rape, rape, yellow mustard, gat, etc., the vitality is weak and greatly affected by the environment. Therefore, the primary hybrid seed production system using autocombination such as cabbage, cabbage, and radish as basic species has not yet been established. However, in rapeseed and gat, agriculturally important crops, cellular male infertility that can be used for seed production of large hybrids has been found, or research on growing large hybrids by introducing Ogura CMS of radish has been actively conducted.

The present inventors induced the Baemuchae into cytoplasmic male infertility (CMS), resulting in a male male infertility system that is fixed enough to be used in one hybrid hybrid. In addition, a variety of varieties of freshly prepared radishes, Ogura CMS and Polima CMS, are first distributed for research purposes.

An object of the present invention is to cultivate and protect a variety of large hybrids using the Baemuchae and Gat CMS system. Another object of the present invention is to provide a single hybrid of apricot CMS and Chinese cabbage, a single hybrid of a fresh CMS and a Chinese cabbage, and a single hybrid of a fresh CMS and a Chinese cabbage.

Another object of the present invention is to provide a first hybrid variety of Chinese cabbage and other species in the Chinese cabbage using the baemuchae and gat CMS.

The above object of the present invention comprises the steps of inducing Baemuchae into cytoplasmic male sterility (CMS); Fostering male sterility (CMS) systems that are fixed to the extent that they are available for one major hybrid (F ₁ ) hybrid; Achievement was achieved by constructing and evaluating various single-hybrid (F ₁ ) plants.

The present invention has an excellent effect of cultivating a variety of large hybrid plants using the cellular male infertility (CMS) system of Baemuchae.

Example 1 Cultivation of Cytoplasmic Male Infertility (CMS) Lines of Baekmuchae and 1 Hybrid Growth

Materials and Methods

As a result of cross-border mating with Baemu-chae and pa-gae as generals, 1.2 seeds per hybridization were obtained (Table 1 above).

[Table 1] Intergenerational Hybridization Results

Therefore, if Baemuchae was made into CMS, it would be possible to cultivate the first hybrid with Gad. Thus, Gat's CMS system was distributed in 2004 by Professor Il Il-sup of Sunchon National University. Then, a bunch of F ₁ seeds was obtained by hybridizing apricot to this CMS lampshade. As a result of flowering this F ₁ , all individuals were male sterility as shown in Table 2.

That is, the male sterility was identified as cytoplasmic male sterility. The fertility of this F ₁ was immediately crosslinked to obtain and bloom seeds of the first generation of female hybrids (BC ₁ F ₁ ). As a result, a child of fertility appeared unexpectedly (Table 3).

The first generation of female inbred hybrids were further hybridized with Baemuchae to obtain and bloom the second generation of female hybrids (BC ₂ F ₁ ). As a result, more temporary households appeared than in the first generation of female hybrids (Table 4). Therefore, it was judged that this cytoplasmic male infertility was not suitable for the debt.

However, as a result of observing infertile individuals from which pollen came out, the number of pollen was remarkably different according to individuals. Some individuals produced the same amount of pollen as pollinate, but some produced very few pollen, which is thought to be sterile. This was defined by the inventors as semi-fertility (FIG. 1). In addition, the complete fertility and semifertility individuals were hybridized to infertile individuals while producing self-pollinating seeds of semifertile individuals. That is, BC ₄ F ₁ seed and BC ₃ F ₂ seed. After cultivation and examination of flowering period, infertile and fertile individuals were also separated in the later generation when hybridized with fertile individuals (Table 5).

However, in later generations hybridized with semi-sterile pollen, only infertile individuals appeared. In the self-pollination of semi-fertile individuals, seeds were produced as fertile individuals, and as a result of flowering, the seeds were separated into infertile and semi-fertile individuals. No fully fertile individuals (Table 6).

Experiment result

In the above experimental method, BC ₄ F ₁ was found to be able to grow and use a fixed male sterility lineage. In other words, it is clear that 100% male infertility can be maintained and expanded by using semi-infertility among the CMS of Baekmuchae. Therefore, tests were carried out for the production of hybrid (F1) seeds by artificial mating of cabbage in male sterile strains (individuals) of Baemuchae (breed) and the radiation of honeybees in the twine. As a result, more seeds were obtained than the self-correction of the debt itself (Tables 7, 8).

Therefore BC ₄ F ₁ BC ₅ F ₁ generation and isolated on LOST with a semi-sterile objects in three and cabbage cultivation and was able to get many seeds as shown in the results in Table 9 by emitting a bee on a flower penis.

As a result of testing (cultivating) the seed of the F ₁ combination thus produced, the purity was uniform in both combinations (Table 10), and one of them was very thick.

This enabled the maintenance and propagation of the CMS using semi-fertility of apricots, and the MS lineages so proliferated to be used as the first hybrid hybrid with Chinese cabbage. Since there are various subspecies among Chinese cabbage species and various strains among Chinese cabbage subspecies, various major hybrid varieties may be grown. By the way, all of these F ₁ varieties are sterile, so that the varieties can be protected.

In the future, it is possible to cultivate a system of aubergine bonds with completely different characteristics. Therefore, this new strain can be used to nurture male sterility strains and the first hybrid of the existing strain. Even in the later stages of the hybrid, even if fertile individuals emerge, new varieties capable of protecting varieties can be nurtured because of the separation of traits.

[Example 2] Fostering large hybrids between freshly CMS line, apricot and cabbage

Hybridization of baewoochae with various Gat-type CMSs resulted in seeds that were less than GAT's self-correction but were able to meet the agricultural management balance (Table 11).

Also, when the cabbage was freshly hybridized to CMS, much more seeds were obtained. Therefore, it is possible to develop a variety of sour vegetable crops by fostering hybrids of gat and baemulchae and hybrids of gat and cabbage. For example, gat has a deep purple red gat and a root gat (bottom) which grows up to 0.7 ~ 1.2kg. And the freshest cabbage and vegetables are the strongest spicy plants, and the ingredients that make up the taste are anti-cancer glucosinolate compounds. Therefore, it is possible to cultivate F1 varieties, etc., with purple radish.

Also, among cabbage varieties, there are some greens (called daa asa) that have very green leaves, and some turnips are dark purple. One-hybrid varieties with enhanced functionality can be grown by hybridizing cabbages, turnips, etc. to the CMS system of red and fresh root CMS.

The present invention is a very useful invention in the vegetable breeding industry because it has an excellent effect to cultivate a variety of large hybrid plants using the cytoplasmic male sterility (CMS) system of Baekmuchae.

1 is a photograph showing male gender, male half infertility, male infertility of new varieties of Baemuchae.

Claims (2)

Inducing the Baemuchae into cytoplasmic male sterility (CMS); Fostering male sterility (CMS) systems that are fixed to the extent that they are available for one major hybrid (F ₁ ) hybrid; A method for breeding intra- and subspecies 1-to-hybrid plants, characterized by the step of growing various 1-hybrid (F ₁ ) plants. The method of claim 1, wherein the variety of the first hybrid plant is a hybrid of Baekmuchae CMS and Chinese cabbage, one hybrid of Gad CMS and Chinese cabbage, one hybrid of Gad CMS and Chinese Cabbage, one of Baemuchae and Gad CMS. A breeding method characterized by any one of the large hybrid plants.

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