CN1307866C - Rape cytoplasmic male sterility + self incompatibility hybrid superiority using method - Google Patents
Rape cytoplasmic male sterility + self incompatibility hybrid superiority using method Download PDFInfo
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- CN1307866C CN1307866C CNB2004100608225A CN200410060822A CN1307866C CN 1307866 C CN1307866 C CN 1307866C CN B2004100608225 A CNB2004100608225 A CN B2004100608225A CN 200410060822 A CN200410060822 A CN 200410060822A CN 1307866 C CN1307866 C CN 1307866C
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Abstract
The present invention relates to a using method of rape cytoplasm male sterility and selfing incompatibility hybrid superiority. A temperature sensitive polCMS is selected as a female parent, and a selfing incompatibility line serves as a male parent. The selfing incompatibility gene is converted to the temperature sensitive polCMS through methods of hybridization, backcrossing, etc., and a selfing incompatibility line which has stable representation of high temperature thorough sterility, low temperature miropowder selfing incompatibility cytoplasm male sterile is selectively bred from descendant. The line serves as a female parent, and is widely hybridized in a test mode with a large quantity of the existing double-low rape breeds at home and abroad, or a maintenance line and a restoring line are screened out and cultured through methods of hybridization and backcross. The incompatibility characteristic of sterility and the selfing of the cytoplasm male sterile and the selfing incompatibility line are kept by a maintenance line. The matching of three lines is realized by the recovering the fertile capability of the cytoplasm male sterility and the self-incompatible line by the restoring line. The using method can be used for reducing the cost and the risk of seed production, and the high hybrid purity and the heterosis strength can be ensured.
Description
Technical field
The invention belongs to rape heterosis and utilize the improvement of method, be specifically related to the not affine three-way cross hybrid use of advantage of a kind of rapeseed cytoplasmic male sterile+selfing new method.Its application can reduce the hybrid seeding risk effectively, improves hybrid purity and hybrid vigour intensity, thereby improves yield of rape, increases income, promotes the development of rape industry.
Background technology
Rape (comprising Wild cabbage type, Chinese cabbage type and juncea) is China and even whole world important food plant oil source, the plant nutrition body of double-low rapeseed both can be used as the edible food utilization that also can be used as of vegetables, the feed protein source that the grouts after its seed oil expression are excellent especially.For a long time, improving per unit area yield is rape research and the primary goal of producing, and heterotic utilization has become irreplaceable, the effective method the most that improves per unit area yield, increases income.Statistics and domestic new varieties regional test result show that the cultivated area of present domestic cross-bred rape surpasses 50%, and the kind overwhelming majority of new seed selection is a crossbreed, and these crossbreed are mainly derived from the male sterile line hybrid.
Rapeseed male sterility has two kinds of nuclear male sterility (GMS) and cytoplasmic male sterilities (CMS).Although the GMS sterility is thorough, stable, but owing to lack maintainer, there is about educated strain of 50% in the sterile line, must remove fertile plant in initial bloom stage during the production of hybrid seeds, this has not only increased breeding cost, and reduced hybrid seed yield, also may remove the not thorough seed production purity that affects owing to educating strain, thereby increase Planting risk.CMS mainly contains pol CMS (pol cytoplasmic male sterility (pol cms)), ogu CMS (radish cytoplasmic male sterilty) etc.Though ogu CMS recovers gene and finds, and transformation is in cabbage type rape, and its restoring force is not strong, and has applied for property right protection, therefore produces the CMS that goes up extensive use and remains the pol CMS the most with practical value that generally acknowledges in the world.But because of the difference of maintainer, pol CMS sterility is subjected to environmental condition impact in various degree, causes its hybrid production application to have certain risk.In addition, recover the restriction in source and may have negative origin and evolution and the low inferior problem of allos pollen affinity, pol CMS continues to utilize prospect to cause anxiety.
The heterosis utilization method also has the self incompatible line hybrid in the rape.The self incompatible line hybrid has following characteristics: 1. the pollen affinity of not affine bitt head is very strong, and the allos setting percentage is very high under the open pollination condition; 2. recover source electrode for extensive.The recovery system that present most cabbage type rape variety is a cabbage type rape of self incompatible lin.But also there is deficiency in the self incompatible line heterosis utilization: 1. there is self-fruitfulness to a certain degree in self incompatible line, and particularly under hot conditions, self-compatibility may be higher; 2. because rape of self incompatible lin pollen is normal, thereby lack form indication traits such as being similar to pollen abortion, this range estimation for hybrid purity is identified and has been brought difficulty.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiency that exists separately in above-mentioned rape pol CMS and the self incompatible line heterosis utilization, collect both length and propose a kind of rapeseed cytoplasmic male sterile+selfing Incompatible hybrid use of advantage method, it can guarantee high hybrid purity and hybrid vigour intensity, reduces breeding cost and risk in hybrid seed production.
The present invention is that the technical scheme that the problem of the above-mentioned proposition of solution adopts is: select the pol CMS of self-compatible responsive to temperature type (to produce trace-pollen under the cryogenic conditions, fully sterile under the hot conditions) the work female parent, make male parent with keeping the male sterile self incompatible line of pol CMS, both hybridization, again by backcrossing, the strange land is bred to add for method and is changed the selfing incompatibility gene pol CMS of responsive to temperature type over to, and stable to show as under the hot conditions no pollen thoroughly sterile in seed selection from the offspring, self-incompatible cytoplasmic male sterility+the self incompatible line of the trace-pollen that produces under the cryogenic conditions; Maternal with cytoplasmic male sterility+self incompatible line work, with the existing in a large number domestic and international extensive test crosses of double-low rapeseed kind, or pass through hybridization, backcross transformation method, screening, cultivation maintainer and restorer; Sterile and the selfing that keeps cytoplasmic male sterility+self incompatible line with maintainer is affinity characteristic not, recovers the fecundity of cytoplasmic male sterility+self incompatible line with restorer, thereby realizes three series mating.
The principles of science of the present invention is: rapeseed cytoplasmic male sterile and selfing are not affine all to be subjected to Gene Handling.The male sterile gene and the selfing incompatibility gene that utilize cytoplasmic male sterility+self incompatible line to carry, it is thoroughly sterile to show WUHUAFEN under the hot conditions, it is not affine that the trace-pollen that cryogenic conditions produces down shows as selfing, thereby assurance no matter is at high temperature or cytoplasmic male sterility+self incompatible line fully can not be solid under cryogenic conditions.Heterosis utilization method principle also promptly is embodied in three series mating method principle, and is identical with existing pol CMS system.
The obtained beneficial effect of the present invention is:
1, this system cells matter male sterile+self incompatible line is because have cytoplasmic male sterility and selfing incompatibility gene, thereby shows as thorough male sterile under the hot conditions, and the trace-pollen that cryogenic conditions produces down shows as selfing can not solid characteristic.Compare with heterosis utilization methods such as existing rape pol CMS, this heterosis utilization new method can improve the hybrid seed yield of hybrid, reduce the production of hybrid seeds risk of original pol CMS and self incompatible line heterosis utilization method, guarantee seed production purity, and then guarantee regularity and the hybrid vigour intensity that hybrid combination is high.
2, improvement or the impact to former pol CMS responsive to temperature type even whole pol CMS system may also comprise: the recovery source enlarges to some extent, and allos pollen affinity strengthens, and the negative origin and evolution that may exist is inhibited, etc.
Embodiment
The specific embodiment of the present invention method is except that cytoplasmic male sterility+selfing not the seed selection difference of parent line, and all the other are all with existing pol CMS heterosis utilization method (see for details＜breeding and the utilization of cross-bred rape〉Fu Tingdong chief editor, 2000).Specifically comprise seed selection, breeding, the production of hybrid seeds and new varieties (hybrid combination) cultivation that cytoplasmic male sterility+selfing not affine three is.
The detailed embodiment division is as follows:
1, the seed selection of cytoplasmic male sterility+self incompatible line: select self-compatible responsive to temperature type (to produce trace-pollen under the cryogenic conditions, fully sterile under the hot conditions) pol CMS does maternal, make male parent with the rape of self incompatible lin that can keep pol CMS male sterility characteristic, both hybridization, hybrid F
1Backcross with male parent again, in backcross progeny, select low temperature that pollen is arranged but selfing is shaky, the complete sterile individual plant of high temperature backcrosses with male parent again, so 3-4 time, by field and laboratory pollen identify, the bagging selfing measures, and cultivates thoroughly sterile, the self-incompatible cytoplasmic male sterility+self incompatible line of low-temperature trace pollen of stable high temperature.Selection is:
The first step: with responsive to temperature type pol CMS is female parent, and the self incompatible line hybridization with keeping pol CMS sterile gene obtains hybrid F
1Generation.
Second step: with the hybrid F that obtains
1On behalf of female parent, with self incompatible line hybridization, obtain the BC that backcrosses
1F
1Generation.
The 3rd step: at BC
1F
1In generation, identify by the bagging selfing, select under the low temperature trace-pollen to be arranged but show as self-incompatible plant and make female parent, continue and self incompatible line hybridization, obtain BC
2F
1Generation.
The 4th step: with the BC that obtains
2F
1Be female parent, self incompatible line is male parent, in 1～2 generation of continuing to backcross, obtains BC
3F
1～BC
4F
1Generation.
The 5th step: at BC
3F
1～BC
4F
1Be tested and appraised in generation and select not affine, the thorough male sterile plant of high temperature of low-temperature fine powder selfing, namely cultivate cytoplasmic male sterility+self incompatible line.
Its pattern is as follows:
Responsive to temperature type pol CMS[S (rfrf) S
RS
R] * self incompatible line [N (rfrf) S
1S
1]
↓ ↓
F
1[S(rfrf)S
RS
1] ↓
↓ ←-backcrosses-
BC
1F
1[S(rfrf)S
1S
1、S(rfrf)S
RS
1]?↓
Select the bagging selfing to show as shaky individual plant [S (rfrf) S
1S
1] ↓
↓ ← continuous backcross
Rapeseed cytoplasmic male sterile+self incompatible line [S (rfrf) S
1S
1].
2, the seed selection of maintainer: with having the not extensive test cross of affinitive material of the good two low type rape pol CMS maintainer of a large amount of Comprehensive Traits and cytoplasmic male sterility+selfing now, if hybrid F
1Keep not affinity characteristic of High-temperature male sterile and low temperature selfing for stablizing, then this kind (being) is maintainer.Can also cultivate new maintainer by hybridization partition method or backcross transformation method: with improved seeds and the hybridization of former maintainer, the continuous selfing of filial generation or with 2-3 again selfing of parent's continuous backcross, selfing or backcross each material while generation and cytoplasmic male sterility+self incompatible line test cross therefrom can filter out stable good new maintainer.The backcross transformation method of maintainer and restorer backcross transformation method are except parent material is different, and detailed process is identical.The hybridization partition method step of maintenance line is:
The first step: it is maternal to select for use improved seeds to do, and with original cytoplasmic male sterility+self incompatible line maintenance line hybridization, obtains F
1In generation,, obtain F with its selfing
2Generation.
Second step: the florescence is at F
2Choose in the colony and select individual plant and cytoplasmic male sterility+self incompatible line test cross, selfing simultaneously, selection can keep the not affine individual plant with the thorough sterile gene of high temperature of the low temperature selfing of cytoplasmic male sterility+self incompatible line, obtain selfing F
3Generation.
The 3rd step: at F
3Select the fine individual plant selfing in generation, simultaneously and male sterile+self incompatible line test cross, therefrom select can stablize the not individual plant of affinity characteristic of the thorough sterile and low-temperature fine powder selfing of the high temperature that keeps male sterile+self incompatible line, obtain selfing F
4Generation.
The 4th step:, repeat more than 2 generations of selfing the good cytoplasmic male sterility+self-incompatible maintainer that can seed selection makes new advances again as the 3rd step.
Its pattern is as follows:
Improved seeds [S or N (rfrf or RfRf) S
MS
MOr S
RS
R] * cytoplasmic male sterility+self-incompatible maintainer [N (rfrf) S
MS
M]
↓
F
1[S or N (rfrf) S
MS
M, S or N (rfrf) SRSM, S or N (Rfrf) S
MS
M, S or N (Rfrf) S
RS
M]
↓ selfing
F
2, at F
2Select individual plant and the simultaneously selfing of cytoplasmic male sterility+self incompatible line test cross in the colony, select to keep thin
Cytoplasmic male sterilty+self incompatible line high temperature is the self-incompatible individual plant of sterile, low temperature (N (rfrf) S thoroughly
MS
M)
↓
F
3, more continuously selfing simultaneously with male sterility+self incompatible line test cross, selects to stablize maintenance male sterility+selfing not
Affine is the not individual plant of affinity characteristic (N (rfrf) S of sterile and selfing
MS
M)
↓
New good cytoplasmic male sterility+self-incompatible maintainer (N (rfrf) S
MS
M)
3, the seed selection of restorer: the one, by the testcross and screening method, with a large amount of existing pol CMS restorer and cytoplasmic male sterility+self incompatible line test crosses with Breeding objective, if hybrid F
1In generation, recovers and can educate, and then this recovery is the restorer of cytoplasmic male sterility+self incompatible line.The 2nd, can be by hybridization or the method transformation that backcrosses, screening with the restorer of Breeding objective: namely hybridize with existing restorer with the good kind of Comprehensive Traits, the continuous selfing of filial generation or with parent's 2-3 again selfing that backcross, simultaneously each selfing or backcross generation and cytoplasmic male sterility+self incompatible line test cross, detect each from generation to generation recovery capability of material, with the new restorer of this seed selection.The selection cross method of restorer is except different from maintainer hybridization partition method parent material, and detailed process is identical.The backcross transformation method step of restorer is:
The first step: it is maternal to select for use improved seeds to do, and with not congenial restoring line hybridization of original cytoplasmic male sterility+selfing, obtains hybrid F
1Generation.
Second step: with the hybrid F that obtains
1Be female parent, one of two parents in the first step are male parent, and both obtain the BC that backcrosses at hybridization
1F
1Generation.
The 3rd step: at BC
1F
1Select individual plant and cytoplasmic male sterility+self incompatible line test cross in the colony, selection can recover the individual plant of cytoplasmic male sterility+self incompatible line fertility and make female parent, and the parent identical with second step backcrosses, and obtains BC
2F
1Generation.
The 4th step: repeat the 3rd and go on foot 1～2 time, selfing is isozygotied again, backcross and selfing while and cytoplasmic male sterility+self incompatible line test cross, each epoch all selects to recover fully the fine individual plant of male sterile+self incompatible line fertility, finally gets final product the recovery system of seed selection cytoplasmic male sterility+self incompatible line that make new advances, that have the breeding objective proterties.
Its pattern is as follows:
Improved seeds [S or N (rfrf or RfRf) S
MS
MOr S
RS
R] * cytoplasmic male sterility+selfing is congenial restoring line [S or N (RfRf) S not
RS
R]
↓
F
1[S or N (Rfrf) S
RS
M, S or N (RfRf) S
RS
M, S or N (Rfrf) S
RS
R, S or N (RfRf) S
RS
R]
↓ backcross with one of them parent
BC
1F
1, at BC
1F
1Select individual plant and the simultaneously selfing of cytoplasmic male sterility+self incompatible line test cross in the colony, select energy
Recover individual plant [S or N (RfRf) S of cytoplasmic male sterility+self incompatible line fertility (pollen can be educated, self-compatible)
RS
R]
↓ continue to backcross with identical (samsara) parent
BC
2F
1, the once or twice that backcrosses again, selfing is isozygotied again, and with male sterility+self incompatible line test cross, selection can be fully extensive simultaneously
Individual plant [S or N (RfRf) S of multiple male sterility+self incompatible line fertility
RS
R]
↓
New, with the cytoplasmic male sterility+selfing of Breeding objective congenial restoring line [S or N (RfRf) S not
RS
R].
4, seed selection and the application of new combination: cytoplasmic male sterility+self incompatible line and a large amount of high-qualitys, the good restorer hybridization of Comprehensive Traits, hybrid F
1Survey the Characters Identifications such as product and quality trait, comprehensive resistance by the field, select that the yield traits vigor intensity is big, the good combinations of proterties such as quality and resistance participate in provincial or national new varieties regional testing and production demonstration test, province or national new varieties authorization mechanism are selected the cross combination definite designation according to above-mentioned result of the test, carry out then large scale application.
5, parent propagation and hybrid seeding that cytoplasmic male sterility+selfing not affine three is: the parent propagation that can the hybrid combination of large scale application on producing and the concrete operations of hybrid seeding are: in isolation condition area preferably, good hybrid combination female parent (cytoplasmic male sterility+self incompatible line) to be applied and male parent (maintenance line or recovery system) are gone than the interval plantation by 4～6: 2, suitably be aided with insect pollinations such as artificial or honeybee at this duration of flowering of father and mother, the whole florescence strikes off male parent, and the maturing stage is gathered in the crops breeding and the cross breeding seed that maternal seed can be realized cytoplasmic male sterility+self incompatible line.The breeding pattern of cytoplasmic male sterility+self incompatible line is:
Cytoplasmic male sterility+self incompatible line [S (rfrf) S
1S
1] * maintainer [N (rfrf) S
MS
M]
↓
Cytoplasmic male sterility+self incompatible line [S (rfrf) S of breeding
1S
1].
The pattern of cytoplasmic male sterility+self incompatible line hybrid seeds is:
Cytoplasmic male sterility+self incompatible line [S (rfrf) S
1S
1] * restorer [S or N (RfRf) S
RS
R]
↓
F
1Hybrid [S or N (Rfrf) S
RS
1].
The determining with the florescence that the two can normally meet and be as the criterion then the same conventional method of daily field management of father, maternal sowing time.The breeding of male parent (maintainer and restorer) is adopted selfing or is that interior brother and sister hand over method, plantation and the same conventional variety of field management, but also must carry out isolation.
Claims (7)
1, a kind of rapeseed cytoplasmic male sterile+selfing Incompatible hybrid use of advantage method, it is characterized in that selecting the pol CMS of self-compatible responsive to temperature type to do maternal, make male parent with keeping the male sterile self incompatible line of pol CMS, both hybridization, again by backcross, the strange land breeds to add for method and changes the selfing incompatibility gene pol CMS of responsive to temperature type over to, seed selection is stable from the offspring shows as the self-incompatible cytoplasmic male sterility+self incompatible line of trace-pollen that no pollen under the hot conditions thoroughly produces under sterile, the cryogenic conditions; Maternal with cytoplasmic male sterility+self incompatible line work, with the existing in a large number domestic and international extensive test crosses of double-low rapeseed kind, or pass through hybridization, backcross transformation method, screening, cultivation maintainer and restorer; Sterile and the selfing that keeps cytoplasmic male sterility+self incompatible line with maintainer is affinity characteristic not, recovers the fecundity of cytoplasmic male sterility+self incompatible line with restorer, thereby realizes three series mating.
2, by the not affine heterosis utilization method of the described rapeseed cytoplasmic male sterile+selfing of claim 1, the seed selection that it is characterized in that described cytoplasmic male sterility+self incompatible line is: select for use and produce self-compatible pol CMS work female parent sterile fully under trace-pollen, the hot conditions under the cryogenic conditions, make male parent with the rape of self incompatible lin that can keep polCMS male sterile characteristic, both hybridization, hybrid F
1Backcross with male parent again, in backcross progeny, select low temperature that pollen is arranged but selfing is shaky, the complete sterile individual plant of high temperature is backcrossed with male parent again, 3～4 times like this, by field and laboratory pollen identify, the bagging selfing measures, and cultivates that stable high temperature is thoroughly sterile, the self-incompatible cytoplasmic male sterility+self incompatible line of low-temperature trace pollen.
3, by claim 1 or 2 described rapeseed cytoplasmic male steriles+selfing Incompatible hybrid use of advantage method, the seed selection that it is characterized in that described maintainer is: with having the not extensive test cross of affinitive material of the good two low type rape pol CMS maintainer of a large amount of Comprehensive Traits and cytoplasmic male sterility+selfing now, if hybrid F
1Keep not affinity characteristic of High-temperature male sterile and low temperature selfing for stablizing, then this kind is maintainer.
4, by claim 1 or 2 described rapeseed cytoplasmic male steriles+selfing Incompatible hybrid use of advantage method, the seed selection that it is characterized in that described maintainer is: cultivate new maintainer by hybridization partition method or backcross transformation method, namely with improved seeds and the hybridization of former maintainer, the continuous selfing of filial generation or with 2～3 again selfings of parent's continuous backcross, selfing or backcross each while generation and cytoplasmic male sterility+self incompatible line test cross, therefrom filter out stable good new maintainer, the hybridization partition method step of maintainer is as follows:
The first step: it is maternal to select for use improved seeds to do, and with original cytoplasmic male sterility+self incompatible line maintenance line hybridization, obtains F
1In generation,, obtain F with its selfing
2Generation;
Second step: the florescence is at F
2Choose in the colony and select individual plant and cytoplasmic male sterility+self incompatible line test cross, selfing simultaneously, selection can keep the not affine individual plant with the thorough sterile gene of high temperature of the low temperature selfing of cytoplasmic male sterility+self incompatible line, obtain selfing F
3Generation;
The 3rd step: at F
3Select the fine individual plant selfing in generation, simultaneously and male sterile+self incompatible line test cross, therefrom select can stablize the not individual plant of affinity characteristic of the thorough sterile and low-temperature fine powder selfing of the high temperature that keeps male sterile+self incompatible line, obtain selfing F
4Generation;
The 4th step:, repeat more than 2 generations of selfing the good cytoplasmic male sterility+self-incompatible maintainer that can seed selection makes new advances again as the 3rd step.
5, by claim 1 or 2 described rapeseed cytoplasmic male steriles+selfing Incompatible hybrid use of advantage method, the seed selection that it is characterized in that described restorer is: by the testcross and screening method, with a large amount of existing pol CMS restorer and cytoplasmic male sterility+self incompatible line test crosses with Breeding objective, if hybrid F
1In generation, recovers and can educate, and then this recovery is the restorer of cytoplasmic male sterility+self incompatible line.
6, by claim 1 or 2 described rapeseed cytoplasmic male steriles+selfing Incompatible hybrid use of advantage method, the seed selection that it is characterized in that described restorer is: namely hybridize with existing restorer with the good kind of Comprehensive Traits, the continuous selfing of filial generation or with parent's 2～3 again selfings that backcross, simultaneously every selfing or backcross generation and cytoplasmic male sterility+self incompatible line test cross, detect each from generation to generation recovery capability of material, with the new restorer of this seed selection, the backcross transformation method step of restorer is as follows:
The first step: it is maternal to select for use improved seeds to do, and with not congenial restoring line hybridization of original cytoplasmic male sterility+selfing, obtains hybrid F
1Generation;
Second step: with the hybrid F that obtains
1Be female parent, one of two parents in the first step are male parent, and both obtain the BC that backcrosses at hybridization
1F
1Generation;
The 3rd step: at BC
1F
1Select individual plant and cytoplasmic male sterility+self incompatible line test cross in the colony, selection can recover the individual plant of cytoplasmic male sterility+self incompatible line fertility and make female parent, and the parent identical with second step backcrosses, and obtains BC
2F
1Generation;
The 4th step: repeat the 3rd and go on foot 1～2 time, selfing is isozygotied again, backcross and selfing while and cytoplasmic male sterility+self incompatible line test cross, each epoch all selects to recover fully the fine individual plant of male sterile+self incompatible line fertility, the recovery system of final seed selection cytoplasmic male sterility+self incompatible line that make new advances, that have the breeding objective proterties.
7, by claim 1 or the not affine heterosis utilization method of 2 described rapeseed cytoplasmic male sterile+selfings, it is characterized in that the production of hybrid seeds of described hybrid combination and the concrete operations of parent propagation are: in isolated area, with good hybrid combination female parent to be applied, it is cytoplasmic male sterility+self incompatible line, with male parent, be maintenance line or recovery system, plant than the interval by 4～6: 2 row, be aided with artificial or honeybee pollination at this duration of flowering of father and mother, the whole florescence strikes off male parent, and the maturing stage is gathered in the crops breeding and the cross breeding seed that maternal seed is promptly realized cytoplasmic male sterility+self incompatible line; The determining with the florescence that the two can normally meet and be as the criterion then the same conventional method of daily field management of father, maternal sowing time; Male parent, the i.e. breeding of maintenance line and recovery system adopt selfing or are that interior brother and sister hand over method, plantation and the same conventional variety of field management, but also necessary the implementation isolated.
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| CN101422133B (en) * | 2008-12-23 | 2011-12-21 | 华中农业大学 | Cabbage type rape self-incompatibility two-line hybrid seed-selection method |
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| CN102150614A (en) * | 2011-04-20 | 2011-08-17 | 华中农业大学 | Method for breeding rape polima cytoplasmic male sterile ( Pol-CMS) line |
| CN104082054B (en) * | 2014-05-28 | 2015-11-18 | 安徽省农业科学院作物研究所 | A kind of method utilizing high temperature stress to identify the sterile pnca gene type of rape |
| CN105052494B (en) * | 2015-08-18 | 2017-11-28 | 山东省潍坊市农业科学院 | The organic cultivation method of ternip |
| CN105284596A (en) * | 2015-10-20 | 2016-02-03 | 河南省粮源农业发展有限公司 | Clustered pod pepper bee pollination three-line hybrid seed production method by setting up net room |
| CN105638447B (en) * | 2016-03-29 | 2017-09-15 | 湖北利众种业科技有限公司 | Improve the selection of Rapeseed GMS system's yield and seed production purity |
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| 青海甘蓝型春油菜波里马细胞质雄性不育三系选育研究 杜德志、刘青元、王宁惠等，青海农林科技，第3期 1996 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422133B (en) * | 2008-12-23 | 2011-12-21 | 华中农业大学 | Cabbage type rape self-incompatibility two-line hybrid seed-selection method |
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