CN1037654C - Rape cell nucleus and cytoplasm male sterility trilinear selection and breeding method - Google Patents
Rape cell nucleus and cytoplasm male sterility trilinear selection and breeding method Download PDFInfo
- Publication number
- CN1037654C CN1037654C CN 93109219 CN93109219A CN1037654C CN 1037654 C CN1037654 C CN 1037654C CN 93109219 CN93109219 CN 93109219 CN 93109219 A CN93109219 A CN 93109219A CN 1037654 C CN1037654 C CN 1037654C
- Authority
- CN
- China
- Prior art keywords
- line
- dgcms
- male
- cms
- sterile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention belongs to the improvement of the heterosis utilization method of rape. The improvement is characterized in that a traditional method is innovated, namely that nuclei and cytoplasmic male sterile (GCMS) three lines are obtained by hybridizing pol or Shan 2A cytoplasmic male sterile (CM) three lines and nuclear male sterile (GMS) two lines; based on the differences of the dominance-recessiveness of nuclear sterile genes, the matching seed production of three lines is respectively carried out according to dominant nuclei and cytoplasmic male sterility (DGCMS), and recessive nuclei and cytoplasmic male sterility (RGCMS). Compared with the prior art, with the improvement of the present invention, the seed production output and the seed production purity can be improved, and the seed production cost and the seed production risks are lowered; thus, a new path for the heterosis utilization of the rape is exploited.
Description
The invention belongs to rape heterosis and utilize the improvement of method, being specifically related to a kind of karyon plus cytoplasmic male sterility seed selection three is the new method of rape.Its international Patent classificating number is A01H1/02.
Studies have shown that in a large number that both at home and abroad rape (comprising the Chinese cabbage type, juncea and Wild cabbage type) intervarietal hybrid exists tangible hybrid vigour.At present, utilize the possible approach of rape heterosis that cytoplasmic male sterility (CMS), nuclear male sterility (GMS), selfing not affine (SI) and chemical emasculation are arranged.Because chemical emasculation exists and easily causes environmental pollution, be difficult to filter out high-efficiency low-toxicity male gametocide and the not high shortcoming of producing of hybrid purity, so the approach of rape heterosis utilization in the world wide mainly is CMS, GMS and SI.The major defect of rape SI is relatively difficulty and the easy affine degree methods of parental autocopulation of identifying intuitively of shortage of parent propagation, and therefore, utilizing SI to produce hybrid in the production has bigger risk.The advantage of rape GMS is that its sterility is very stable, but needs in male sterile line to pull out in initial bloom stage 50% educated strain when the production of hybrid seeds, not only can increase production of hybrid seeds input thus, reduce hybrid seed yield, and, can influence seed production purity often owing to can educate the not thorough of strain removing, increase production risk.Because there are such and such shortcoming in GMS, SI and chemical emasculation, now most of in the world rapeseed breeding men are utilizing the attention diversion of rape heterosis on cytoplasmic male sterility.At present, there are ogu and Pori horse (or Shan 2A) in the CMS system that can be used for the rape heterosis breeding in the world, the major defect of ogu CMS system is the recovery gene that can not find it in rape, be difficult to realize three series mating, although at present countries such as Canada and France from radish the recovery gene transfer of ogu CMS to rape, its recovery is to exist restoring force not by force and serious problems such as female sterile.Pori horse (or Shan 2A) CMS is the CMS system the most with practical value that generally acknowledges in the world at present, however, the fertility of its male sterile line is subjected to environmental condition influence in various degree because of the difference of its maintenance line, the subject matter of bringing thus is: 1, in the seed selection process of male sterile line, to hand over and selection with paired through backcrossing of continuous multi-generation, just can select the metastable male sterile line of fertility (not being absolute stability), breeding cycle is long, cost is high.2, owing to exist backcrossing, hand in pairs and selecting of continuous multi-generation in the breeding process of male sterile line, must force its maintenance line continuous multi-generation selfing, and constantly select, thereby cause selfing to be degenerated, reduced the vitality and the coordinate force of its parent (male sterile line and maintenance line).3, because the male sterile line that selects is just relatively stable on male sterility, when climatic variation, cultivation condition variation or the breeding of process several generations, the fertile flower powder that number does not wait can appear in male sterile line, so utilize this CMS system to produce hybrid on producing certain risk is arranged.
The objective of the invention is to overcome the prior art deficiency, provide a kind of and can improve hybrid seed yield and seed production purity, reduce the method for the rape heterosis utilization of breeding cost and production of hybrid seeds risk.
The present invention can be achieved through the following technical solutions:
A kind of rape heterosis utilizes method, comprise and select for use cytoplasmic male sterility (CMS) three to be and nuclear male sterility (GMS) two is and matching method, the seed selection of its CMS maintenance line is to select for use to have the material and the material hybridization that has the breeding objective proterties that CMS keeps gene, isolate the CMS maintenance line that meets breeding objective in its filial generation, the breeding of CMS maintenance line is adopted selfing and is that interior brother and sister hand over; CMS recovers the seed selection of system and selects material with CMS recovery gene and the material hybridization that has the breeding objective proterties for use, in its offspring, isolate the CMS that meets breeding objective and recover system, CMS recovers the breeding employing selfing of system and is that interior brother and sister hand over, described method be adopt Pori horse or Shan 2ACMS three to be and show, the recessive karyon two-line male sterile is material, change traditional selection into karyon plus cytoplasmic male sterility (GCMS) three line breeding, the method that breeding and hybrid produce.
Be described in detail details of the present invention below:
One, dominant karyon+cytoplasmic male sterility (DGCMS) three seed selection of being, breeding and hybrid production
(1) DGCMS three seed selection that is
1, the seed selection of DGCMS maintenance line
Because the genotype of DGCMS maintenance line is identical with the CMS maintenance line, is N (ms
1Ms
1RfnrfnrfcrfcTsTs...), therefore, its selection is identical with the CMS maintenance line.
2, the seed selection of DGCMS male sterile line
The selection of DGCMS male sterile line is for its maintenance line, and comparatively complicated, its seed selection can be carried out according to the following steps.The principle of DGCMS sterile line breeding is to show as the sterile individual plant of matter in the DGCMS male sterile line that selects of requirement not have pollen or denier pollen is arranged.
Make the male parent continuous backcross with the DGCMs maintenance line, eliminate to make backcross progeny show as the DGCMS maintenance line (or individual plant) that more pollen appears in Pori's sterile individual plant of horse CMS, the individual plant of selecting for use the DGCMS that can make the sterile individual plant of the performance horse CMS of Pori in the backcross progeny denier pollen only occur to protect in the system continues to backcross.
↓
BC
8F
11(DGCMS male sterile line) 1/2S (MS
1Ms
1RfnrfnrfcrfcTsTs...)+1/2S (ms
1Ms
1RfnrfnrfcrfcTsTs...)
(matter sterile+nuclear sterile) (matter is sterile)
3, DGCMS recovers the seed selection of system
At first select for use to have the cytoplasmic CMS of Pori horse and recover system and do maternally to recover system's hybridization with DGM1S, isolate DGCMS then and recover system in its self progeny, its preference pattern is as follows:
CMS recovers (the ♀) * DGMS of system and recovers system (♂)
S(ms
1ms
1rfnrfnRfcRfcTsTs...)↓N(ms
1ms
1RfnRfnrfcrfcTsTs...)
F
1Selfing
S(ms
1ms
1RfnrfnRfcrfcTsTs...)
↓
F
2
Individual plant (the F that can make DGCMS male sterile line test cross offspring absolutely recover fertility is selected in sterile strain test cross of nuclear in F2 colony in roguing and the DGCMS male sterile line and selfing simultaneously
2) selfed seed (F
3) continue to sow.
1/16S(ms
1ms
1RfnRfnRfcRfcTsTs...)
↓
F
3
S(ms
1ms
1RfnRfnRfcRfcTsTs...)
Continue selfing and select, simultaneously with the DGCMS male sterile line in the sterile test cross of nuclear, selection can make it complete
The individual plant of recovery fertility ↓
↓
F
6
DGCMS recovers system
S(ms
1ms
1RfnRfnRfcRfcTsTs...)
″
(2), DGCMS three breeding that is
1, the maintenance line of DGCMS is exactly the maintenance line of DGCMS, so the breeding of DGCMS maintenance line and CMS maintenance line is identical.
2, the breeding of DGCMS male sterile line
With the alternate plantation of DGCMS male sterile line with the DGCMS maintenance line, in the DGCMS male sterile line, pull out 50% when just spending and show as the sterile individual plant of the horse CMS of Pori, on the DGCMS male sterile line, gather in the crops DGCMS male sterile line of future generation during seed maturity, the steps include:
3, DGCMS recovers the breeding of system
From existing DGCMS recover to select the system typical stalwartness can educate the strain selfing and simultaneously with the DGCMS male sterile line in the sterile strain test cross of nuclear, selection can make the DGCMS male sterile line recover the individual plant mixed seeding of fertility fully, breeds DGCMS of future generation and recovers system.
(3) the DGCMS three-way cross hybrid is produced
The DGCMS male sterile line is recovered system with DGCMS press certain row, on the DGCMS male sterile line, gather in the crops hybridization F during seed maturity than alternate plantation
1Seed.
Two, recessive karyon plus cytoplasmic male sterility (RGCMS) three seed selection that is, breeding and hybrid production
(1), the seed selection of RGCMS maintenance line
Selecting for use after good CMS maintenance line does the sterile strain hybridization of nuclear in male parent and the recessive GMS male sterile line, can carry out the seed selection of RGCMS maintenance line as follows.Clearly, the seed selection of RGCMS maintenance line than DGCMS maintenance line seed selection complexity many.
Observe male fertile separation case in the F3 strain system, choosing can be educated strain and sterile strain test cross (handing in pairs) in the F3 strain system that has fertility to separate
1/2N(ms
2ms
2rfcrfcTsTs...)(♀)×1/2N(MS
2ms
2rfcrfcTsTs...)
↓
F
4
Be carried out to continuously handing over, select the stable RGCMS maintenance line of proterties.
↓
↓
F
8
The RGCMS maintenance line
1/2N(ms
2ms
2rfcrfcTsTs...)+1/2N(MS
2ms
2rfcrfcTsTs...)
2, the seed selection of RGCMS male sterile line
Sterile with CMS is maternal, makes the recurrent parent continuous backcross with the RGCMS maintenance line that selects, and eliminates those and make the sterile strain of CMS produce the RGCMS maintenance line of many pollen in backcross process, the steps include:
3, RGCMS recovers the seed selection of system
Because CMS recovers the genotype of system and recovers the identical of system with RGCMS, therefore, the selection that RGCMS recovers system recovers the identical of system with CMS.
(2) breeding of RGCMS maintenance line
1, the breeding of RGCMS male sterile line and maintenance line
With the alternate plantation with the RGCMS maintenance line of RGCMS male sterile line, initial bloom stage pulls out 50% and shows as the sterile individual plant of CMS in the RGCMS male sterile line, the RGCMS male-sterile seed of results breeding in the sterile strain of nuclear when ripe in the RGCMS male sterile line.Simultaneously, can gather in the crops the RGCMS maintenance line seed of breeding in the sterile strain of the nuclear in the RGCMS maintenance line.The propagation method of this RGCMS maintenance line is more more economical than the method for breeding the RGCMS maintenance line separately.Its step is as follows:
2.RGCMS recover the breeding of system
Is identical because of RGCMS recovers system with the CMS recovery, so its modes of reproduction is also recovered the identical of system with CMS.
(3) the RGCMS three-way cross hybrid is produced
The same with CMS, GMS with DGCMS male sterile line type, utilize RGCMS three to produce hybrid, only needing RGCMS male sterile line and recovery is alternate plantation, gathers in the crops hybrid seed when ripe on the RGCMS male sterile line.The steps include:
Compare with existing technology, major advantage of the present invention is:
1. compare with rape GMS, when producing hybrid F1, do not need to pull out 50% educated strain in male sterile line, seeding technique is simpler, thus, not only can improve hybrid seed yield greatly, reduce production costs, and its seeding technique is easily grasped by common peasant.
2. compare with Pori horse (or Shan 2A) CMS, (1) is again that nuclear is sterile owing to the plant that has 50% in the GCMS male sterile line that selects is that matter is sterile, and its sterility degree is strengthened, and stability improves, thereby can improve seed production purity.(2) in GCMS sterile line breeding process, owing to can allow the sterile strain of matter in the male sterile line to show trace-pollen, thereby reduced and requiredly in the breeding of former CMS male sterile line backcrossed, handed in pairs generation number, shorten breeding cycle, reduce the parental autocopulation degree, reduce selfing and degenerate, thereby improved the chance that selects high-combining ability parent (male sterile line and maintenance line).(3) in parent propagation and production of hybrid seeds process, just in case if because weather, cultivation condition or biology hybrid, make the sterile strain of matter in its male sterile line show more pollen when becoming partial sterility and maybe can educate (promptly by sterile), only need in male sterile line, to pull out those during the production of hybrid seeds and show the individual plant that partial sterility maybe can be educated at initial bloom stage, or destroy the main inflorescence that those show the partial sterility individual plant, can the production of hybrid seeds be destroyed as CMS.(4) because GCMS has the advantage of above-mentioned (1) and (3), thereby significantly reduce the loss that hybrid produces, reduce production risk.
Claims (8)
1. a rape heterosis utilizes method, comprise and select for use cytoplasmic male sterility (CMS) three to be and nuclear male sterility (GMS) two is and matching method, wherein the seed selection of CMS maintenance line is to select for use to have the material and the material hybridization that has the breeding objective proterties that CMS keeps gene, isolate the CMS maintenance line that meets breeding objective in its filial generation, the breeding of CMS maintenance line is adopted selfing and is that interior brother and sister hand over; CMS recovers the seed selection of system and selects material with CMS recovery gene and the material hybridization that has the breeding objective proterties for use, in its offspring, isolate the CMS that meets breeding objective and recover system, CMS recovers the breeding employing selfing of system and is that interior brother and sister hand over, it is characterized in that, described method be adopt Pori horse or Shan 2ACMS three to be and show, the recessive karyon two-line male sterile is material, carry out karyon plus cytoplasmic male sterility (GCMS) three line breeding, breeding and hybrid produce.
2. method according to claim 1 is characterized in that, will contain GCMS material apparent, recessive gene and carry out three series mating according to dominant karyon+cytoplasmic male sterility (DGCMS) and recessive karyon plus cytoplasmic male sterility (RGCMS) respectively.
3. method according to claim 1 and 2 is characterized in that, the selection that described DGCMS three is comprises:
A. adopt following program seed selection DGCMS maintenance line;
The first step: do male parent or kind (being) hybridization maternal and that have the breeding objective proterties with the CMS maintenance line, obtain F
1Generation.
Second step: with the F that obtains
1Selfing obtains F
2Generation.
The 3rd step: from the F that obtains
2Select the fine individual plant selfing in the colony, obtain F
3Generation, and make male parent and DGCMS male sterile line test cross with it simultaneously, florescence next year is identified test cross offspring's fertility.
The 3rd step: with F
2The F that has the individual plant of good maintenance effect in the colony
3For planting seed, eliminate the seed that keeps weak effect.
The 4th step: from F
3To F
6Generation, in per generation, selected good individual plant selfing from colony, and make male parent and DGCMS male sterile line test cross with elected individual plant simultaneously, and florescence next year is identified its test cross offspring's fertility, therefrom filter out the individual plant that proterties is good and have good maintenance effect, obtain the DGCMS maintenance line at last.
B. adopt following program seed selection DGCMS male sterile line:
The first step: doing maternal the recovery with dominance GMS with dominance GMS male sterile line is hybridization, obtains F
1Generation.
Second step: with the F that obtains
1In generation, done male parent and the hybridization of CMS male sterile line, obtains F
11Generation.
The 3rd step: with the F that obtains
11In the sterile strain of caryoplasm be maternal and the hybridization of DGCMS maintenance line, obtain BC
1F
11Generation.
The 4th step: from the BC that obtains
1F
11Select the sterile strain of caryoplasm to make female parent in the colony and backcross, obtain BC with the DGCMS maintenance line
2F
11Generation.
The 5th step: keep from backcross progeny (BC
2F
11To BC
5F
11) the sterile strain of middle selection caryoplasm does maternal and the DGCMS maintenance line is backcrossed 4 times.In backcross process, eliminating those makes backcross progeny show as the DGCMS maintenance line (or individual plant) that more pollen appears in the sterile individual plant of CMS, select for use the individual plant in the DGCMS maintenance line that can make in the backcross progeny the sterile individual plant of performance CMS denier pollen only occur to continue to backcross, obtain DGCMS male sterile line and corresponding maintenance line thereof.
C. adopt following program seed selection DGCMS to recover system:
The first step: do maternal and the hybridization of dominance GMS recovery system to have the cytoplasmic CMS recovery of CMS system, obtain F
1Generation.
Second step: with the F that obtains
1Selfing obtains F
2Generation.
The 3rd step: sterile strain test cross of the caryoplasm in F2 colony in roguing and the DGCMS male sterile line and selfing simultaneously, the selfed seed (F3) of selecting the individual plant (F2) that can make DGGMS male sterile line test cross offspring absolutely recover fertility continues sowing.
The 4th step: continue selfing and select 4 times, simultaneously with the DGCMS male sterile line in the sterile strain test cross of caryoplasm, select and can make its individual plant that recovers fertility fully, obtain DGCMS and recover system.
4. method according to claim 3, the propagation method that DGCMS three is is:
A. adopt following program breeding maintenance line;
Isolate or natural isolation condition is sowed and planted the DGCMS maintenance line artificial,, when ripe seed mixed and receive respectively seedling stage and florescence impurity elimination strain and weak plant.
B. adopt following program breeding DGCMS male sterile line:
With the alternate plantation of DGCMS male sterile line with the DGCMS maintenance line, in the DGGMS male sterile line, pull out 50% when just spending and show as the sterile individual plant of CMS, on the DGCMS male sterile line, gather in the crops DGCMS male-sterile seed of future generation during seed maturity.
C. adopt following program breeding DGCMS to recover system:
The first step: recover to select in the system typical healthy and strong individual plant and DGCMS male sterile line test cross to obtain F at DGCMS
1Generation, and with its selfing.
Second step: the florescence is identified F
1The male fertile in generation.
The 3rd step: recover to select the typical individual plant mixed seeding that can absolutely make the DGCMS male sterile line recover fertility the system from DGCMS, breed DGCMS and recover system.
5. method according to claim 3 is characterized in that the program that three-way cross hybrid is produced comprises: the DGCMS male sterile line is recovered system with DGCMS press certain row than alternate plantation, gather in the crops hybridization F1 seed during seed maturity on the DGCMS male sterile line.
6. method according to claim 1 and 2 is characterized in that, described RGCMS three line breeding method comprises:
A. adopt following program seed selection RGCMS maintenance line:
The first step: with the sterile strain in the recessive GMS male sterile line is maternal and the hybridization of CMS maintenance line, obtains F
1Generation.
Second step: with the F that obtains
1For selfing, obtain F
2Generation.
The 3rd step: from F
2Can educate strain and make male parent and F for selecting in the colony
2In the sterile strain of nuclear be carried out to handing over (brother and sister's friendship), obtain F
3Generation.
The 4th step: with F
3In generation,, florescence investigation male fertile was at the F that has fertility to separate by handing over the sowing of combination plant division system in pairs
3Select to educate strain in the strain system and make male parent and this F
3The sterile strain test cross of nuclear in the strain system (handing in pairs) obtains F
4Generation.
The 5th step: continue at F
4, F
5And F
6In be carried out to handing over according to the method in the 4th step, therefrom select the stable RGCMS maintenance line of proterties.
B. adopt following program seed selection RGCMS male sterile line:
The first step: do RGCMS maintenance line hybridization maternal and that select with the CMS male sterile line, obtain F
1Generation.
Second step: with the F that obtains
1Backcross with corresponding RGCMS maintenance line for maternal, obtain BC
1Generation.
The 3rd step: eliminate BC
1The many combinations of pollen appear in the sterile strain of middle performance CMS, at elected BC
1Select the sterile strain continuation of caryoplasm to backcross in the combination and obtain BC with corresponding RGCMS maintenance line
1Generation.
The 4th step: from BC
2To BC
5Generation, continue to eliminate the combination that more pollen appears in the sterile strain of CMS, in elected combination, select the sterile strain of caryoplasm to backcross with corresponding RGCMS maintenance line, obtain RGCMS male sterile line and corresponding maintenance line thereof.
7. method according to claim 6, it is characterized in that, adopt male sterile line and the maintenance line of following steps breeding RGCMS: with the alternate plantation of RGCMS male sterile line with the RGCMS maintenance line, initial bloom stage pulls out 50% and shows as the sterile individual plant of CMS in the RGCMS male sterile line, the numerous RGCMS male-sterile seed of results in the sterile strain of nuclear when ripe in the RGCMS male sterile line, simultaneously, can gather in the crops the RGCMS maintenance line seed of breeding in the sterile strain of the nuclear in the RGCMS maintenance line.
8. method according to claim 6, the production method that it is characterized in that its three-way cross hybrid is gathered in the crops hybrid seed for being alternate plantation with RGCMS male sterile line and recovery when ripe on the RGCMS male sterile line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93109219 CN1037654C (en) | 1993-08-05 | 1993-08-05 | Rape cell nucleus and cytoplasm male sterility trilinear selection and breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93109219 CN1037654C (en) | 1993-08-05 | 1993-08-05 | Rape cell nucleus and cytoplasm male sterility trilinear selection and breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1098247A CN1098247A (en) | 1995-02-08 |
| CN1037654C true CN1037654C (en) | 1998-03-11 |
Family
ID=4987540
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93109219 Expired - Fee Related CN1037654C (en) | 1993-08-05 | 1993-08-05 | Rape cell nucleus and cytoplasm male sterility trilinear selection and breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1037654C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095329C (en) * | 1996-01-22 | 2002-12-04 | 贵州省油料科学研究所 | Method for plantation of rape seeds of two lines with recessive nuclear sterility |
| CN1072888C (en) * | 1996-10-24 | 2001-10-17 | 四川省农业科学院作物研究所 | Tech. for improving pureness of three incross rapeseed |
| CN1073343C (en) * | 1996-10-28 | 2001-10-24 | 四川省农业科学院作物研究所 | Selective breeding for pit-sterility cross-bred rape and seed-making method thereof |
| CN1105491C (en) * | 1997-12-23 | 2003-04-16 | 安徽省农业科学院作物研究所 | Cabbage type rape recessive nueleus sterility triseries breeding and technology for production of seeds |
| CN104285778A (en) * | 2014-09-26 | 2015-01-21 | 华中农业大学 | Method for breeding ecotype recessive nuclear male sterile two-purpose line of oilseed rape |
-
1993
- 1993-08-05 CN CN 93109219 patent/CN1037654C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1098247A (en) | 1995-02-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2021190131A1 (en) | Breeding method for three-line hybrid combination of colored rice | |
| CN102217526B (en) | A kind of selection of black barley | |
| CN1762199A (en) | Method for obtaining cytoplasm male sterility three lines from paddy TGMS(thermo-sensitive genic male sterile) line | |
| EP4342289A1 (en) | Breeding method of blue marked two-line hybrid wheat system and use | |
| CN105409762A (en) | Cyclic breeding method of cotton varieties | |
| CN1037654C (en) | Rape cell nucleus and cytoplasm male sterility trilinear selection and breeding method | |
| CN101720665A (en) | Method for breeding male sterile line in ternary hybrid rice with orange red mark property | |
| CN104082128A (en) | Reproductive method of intelligent rice sterile line | |
| CN1127893C (en) | Two-line male sterile method for assortively breeding ecological-type cytoplasm of rape | |
| CN103348908B (en) | Method for selecting hybrid seeds of Brassica juncea var. multiceps | |
| CN1307866C (en) | Rape cytoplasmic male sterility + self incompatibility hybrid superiority using method | |
| CN1843091A (en) | Rape cytoplasm male sterility three line selection and breeding method | |
| CN111406644B (en) | Cluster-growing pod pepper nuclear-cytoplasmic interaction type male sterile line breeding method and application of three-line matched system | |
| CN105613257B (en) | A kind of samsara selection of cotton sterile line | |
| CN1531843A (en) | Novel cabbage type rape cytoplasmic male sterile line material of Chinese cabbage vegetable transformation and its obtaining method | |
| CN1105491C (en) | Cabbage type rape recessive nueleus sterility triseries breeding and technology for production of seeds | |
| CN102919116B (en) | Selection breeding method of new homozygous sterile line of brassica napus | |
| CN1065712C (en) | Hybrid vigor fixing breeding process for rice apomixis | |
| McKenzie | Fertility Relationships Among Interspecific Hybrid Progenies of Gossypium 1 | |
| CN110951906A (en) | High generation backcross Molecule Recurrent Selection (MRSAB) breeding method I-a method for cultivating and utilizing dominant early panicle non-yield-reduction material | |
| CN1273008C (en) | Rice broad-spectrum wide-compatibility restoring line breeding method | |
| CN1141017C (en) | Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China | |
| CN110786233B (en) | Method for separating and breeding homogeneous temporary protection and homozygous sterile line by using nuclear three-line rape F2 generation | |
| CN1156211C (en) | Light-sensitive male sterility line of rape and hybrid seed breeding method thereof | |
| CN1179635C (en) | Method for breeding yellow-seed high oil content rape or cytoplasm male sterility recovery line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |