CN1095329C - Method for plantation of rape seeds of two lines with recessive nuclear sterility - Google Patents
Method for plantation of rape seeds of two lines with recessive nuclear sterility Download PDFInfo
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- CN1095329C CN1095329C CN96102204A CN96102204A CN1095329C CN 1095329 C CN1095329 C CN 1095329C CN 96102204 A CN96102204 A CN 96102204A CN 96102204 A CN96102204 A CN 96102204A CN 1095329 C CN1095329 C CN 1095329C
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Abstract
The present invention discloses a seed production method of cabbage type rape recessive nuclear sterile two lines. In the seed production method, parents used for the seed production are original seeds reproduced by breeder seeds; double-low recessive nuclear sterile dual-purpose line parent 22A original seeds are used as a female parent, and restoring line 1536-119 original seeds are used as a male parent; by a matching seed production cultivation technique, a double-low hybridization rape variety 'Youyan No. 7' is obtained. The 'Youyan No. 7' of the present invention has the advantages that the recovery sources are wide, and high heterosis combinations are easily selected and matched; the time taken is short, the effects are good, the quality of seeds produced is easily kept, etc. In the demonstration cultivation, the average yield per mu can reach 189.2 kilograms; compared with 'Qinyou No. 2', the average yield per mu can be increased by 18% or so.
Description
Technical field: the cabbage type rape recessive cytoblast sterile two that the present invention relates to a kind of low erucic acid, low sulfatide cross-bred rape kind is the method for the production of hybrid seeds, belongs to cabbage type rape recessive cytoblast sterile double-line hybrid advantage seeding technique field.
Background technology: at present, in crop hybrid superiority utilized, utilizing its male sterile to carry out hybrid seeding was the most common fundamental method.For example " No. two, Qin's oil " is exactly the high-quality hybrid rape variety that adopts heterosis, hybrid vigor to nurture.In the prior art, rapeseed male sterility divides karyon male sterile and cytoplasmic male sterilty.Sterile for kytoplasm, the unit of its research is more, and realized three series mating and be used for production, but be subjected to environmental influences such as temperature can produce trace-pollen because of its male sterile line, the difficulty of guaranteeing seed production quality is very big, the region of the suitable autumn sowing production of hybrid seeds is extremely strong, thereby it uses popularization still to be subjected to certain restriction; The karyon male sterile material is with respect to the cytoplasmic male sterilty material, though have stable fertility, sterility thoroughly and be difficult for advantages such as affected by environment, but because the sterile gene of this type of material is a dominance, the recovery source is less, the survey that influences strong excellent combination is joined, from and influenced seed production quality, so the research work of this respect imperfection also also is in stage of further preparing hybrid combination.
Summary of the invention: it is wide to the purpose of this invention is to provide a kind of recovery source, cabbage type rape that the production of hybrid seeds is easily guaranteed the quality recessive sterile two is the method for the production of hybrid seeds, makes the cross-bred rape kind that nurtures by this method reach content of erucic acid and glucosinolate content is all low and output is higher purpose.
The technical solution adopted in the present invention is: it comprises distance isolation, proper seeding, roguing, extract female parent and can educate strain, artificial supplementary pollination and parent and the measure of production of hybrid seeds results, the parent of the production of hybrid seeds all is the original seeds by the breeder's stock breeding when the production of hybrid seeds, it is maternal to select for use the two dual-purpose parent of the being 22A of low recessive cytoblast sterile original seeds to do, recovery is that the 1536-119 original seed is made male parent, and make the cross-bred rape kind by the matched breeding culture technique, its maternal 22A is the two low recessive gms line that utilizes the recessive sterile material 117A of low mustard to become with two No. two hybridization transformations in two low kinds, breed by breeder's stock, and the original seed of in the sterile strain of florescence mark, being received, its parental stock requires content of erucic acid less than 1%, glucosinolate content is less than 30 micromoles, sterile plant rate is greater than 45% in the female parent, plant typical case proterties conforms to parent's character requirement, its male parent 1536-119 from the recovery that low erucic acid low sulfatide 821 selects seed selection to become through system oriented is, its original seed content of erucic acid is less than 1%, and glucosinolate content is less than 30 micromoles; Be used for parent's breeder's stock of parental stock breeding, its content of erucic acid is less than 0.5%, and glucosinolate content is less than 25 micromoles, and sterile plant rate is greater than 48%; The breeding of parental stock requires distance isolation more than 3 kilometers, and production of hybrid seeds distance isolation is not planted other any cruciate flower material crop more than 1.5 kilometers in the isolated area, and wherein production of hybrid seeds isolated area can be planted male parent; Before and after first flower, in time pull out the educated strain in the female parent, require pull out can educate strain before every strain bloom and can not surpass 2-3, can educate the educated strain of not pulling out after strain finishes and can not surpass 1% of remaining maternal sterile strain sum pulling out; When parent and production of hybrid seeds results, must gather in respectively according to the situation of the mark of sterile strain or this plantation of seed farm father and mother, pile up separately, threshing respectively, the wind of listing shines and preserves respectively.
Owing to adopted technique scheme, the present invention compared with prior art, the present invention has adopted cabbage type rape recessive karyon male sterile material, thereby it is wide to have a recovery source, the easily strong excellent combination of apolegamy, and the used time is short effective, and production of hybrid seeds advantage such as easily guarantee the quality, its content of erucic acid of educating the cross-bred rape kind of making is below 2%, glucosinolate content is less than 30 micromoles, its output is in 3900 mu demonstration plant, 189.2 kilograms of average yield per mus, the highest per mu yield reaches 229.9 kilograms, compare with two " No. two, Qin's oil " with the field contrast, average every mu of volume increase about 18%, breeding time is than " Qin oil No. two " precocious 7-10 days, and the cross-bred rape kind that adopts the present invention to make also has the anti-strong advantage of premunition of falling.
Embodiment: the selection in distance isolation district preferably is selected in little basin of the intermountain that is embosomed in hills or ramp ground, if be chosen in the Pingba area production of hybrid seeds, then the isolation distance of production of hybrid seeds production area requires to be twice above than the isolation distance in the intermountain production of hybrid seeds, in the production of hybrid seeds and isolated area, except that finishing production of hybrid seeds area according to plan, do not allow to plant other rape variety and all cruciate flower material crops; The seeding and growing seedling phase preferably by male parent be selected in 1-September 3 September sowing, female parent is selected in sowing on 7-September 10 in September, after planting with Belmark, flolimat in accordance with regulations multiple spray native face once, prevent that soil insect from encroaching on seed; After the dish seedling has the 4-5 leaf, on the seedbed, carry out a roguing, extract the prosperous especially big seedling of growing way, special-shaped seedling, can guarantee production of hybrid seeds parent's purity after these seedlings are extracted, improve the sterile plant rate in the female parent; When the dish seedling just can be transplanted during at the 5-6 leaf, successively plant male parent earlier according to growing seedlings, after plant female parent, the male parent phase of planting should finish before mid-October, the female parent phase of planting should plant in late October; To note when managing in time at the florescence, strict, extract educated strain in the female parent up hill and dale, and perform artificial supplementary pollination work, artificial supplementary pollination preferably begins to carry out when rape is about to enter full-bloom stage, carries out once every 2-3 days; The parent of the production of hybrid seeds all is the original seeds by the breeder's stock breeding when the production of hybrid seeds, select for use the recessive sterile dual-purpose parent of low erucic acid low sulfatide 22A original seed to do maternal, recovery is that the 1536-119 original seed is made male parent, and press matched breeding culture technique preparing hybrid rape variety, its maternal 22A is the low erucic acid low sulfatide recessive gms line that utilizes the recessive sterile material 117A of low mustard to become with two No. two hybridization transformations in the low erucic acid low sulfatide kind, breed by breeder's stock, and the original seed of in the sterile strain of florescence mark, being received, its parental stock requires content of erucic acid less than 1%, glucosinolate content is less than 30 micromoles, sterile plant rate is greater than 45% in the female parent, and plant typical case proterties conforms to parent's character requirement; Be used for parent's breeder's stock of parental stock breeding, its content of erucic acid is less than 0.5%, and glucosinolate content is less than 25 micromoles, and sterile plant rate is greater than 48%; Male parent 1536-119 selects the recovery of seed selection one-tenth to be from low erucic acid low sulfatide 821 through system oriented, and its original seed content of erucic acid is less than 1%, and glucosinolate content is less than 30 micromoles; The breeding of parental stock requires distance isolation more than 3 kilometers, and production of hybrid seeds distance isolation is not planted other any cruciate flower material crop more than 1.5 kilometers in the isolated area, and wherein production of hybrid seeds isolated area can be planted male parent; Before and after first flower, in time pull out the educated strain in the female parent, require pull out can educate strain before every strain bloom and can not surpass 3, can educate the educated strain of not pulling out after strain finishes and can not surpass 1% of remaining maternal sterile strain sum pulling out; When parent and production of hybrid seeds results, must gather in respectively according to the situation of the mark of sterile strain or this plantation of seed farm father and mother, pile up separately, threshing respectively, the wind of listing shines and preserves respectively.
Claims (5)
1, a kind of cabbage type rape recessive cytoblast sterile two is the method for the production of hybrid seeds, it comprises distance isolation, proper seeding, roguing, extract female parent and can educate strain, artificial supplementary pollination and parent and the measure of production of hybrid seeds results, it is characterized in that: the parent of the production of hybrid seeds all is the original seeds by the breeder's stock breeding when the production of hybrid seeds, it is maternal to select for use the two dual-purpose parent of the being 22A of low recessive cytoblast sterile original seeds to do, recovery is that the 1536-119 original seed is made male parent, and make the cross-bred rape kind by the matched breeding culture technique, its maternal 22A is the two low recessive gms line that utilizes the recessive sterile material 117A of low mustard to become with two No. two hybridization transformations in two low kinds, breed by breeder's stock, and the original seed of in the sterile strain of florescence mark, being received, its parental stock requires content of erucic acid less than 1%, glucosinolate content is less than 30 micromoles, sterile plant rate is greater than 45% in the female parent, plant typical case proterties conforms to parent's character requirement, its male parent 1536-119 from the recovery that low erucic acid low sulfatide 821 selects seed selection to become through system oriented is, its original seed content of erucic acid is less than 1%, and glucosinolate content is less than 30 micromoles.
2, the cabbage type rape recessive cytoblast sterile two according to claim 1 method that is the production of hybrid seeds, it is characterized in that: be used for parent's breeder's stock of parental stock breeding, its content of erucic acid is less than 0.5%, and glucosinolate content is less than 25 micromoles, and sterile plant rate is greater than 48%.
3, the cabbage type rape recessive cytoblast sterile two according to claim 1 method that is the production of hybrid seeds, it is characterized in that: the breeding of parental stock requires distance isolation more than 3 kilometers, production of hybrid seeds distance isolation is more than 1.5 kilometers, do not plant other any cruciate flower material crop in the isolated area, wherein production of hybrid seeds isolated area can be planted male parent.
4, the cabbage type rape recessive cytoblast sterile two according to claim 1 method that is the production of hybrid seeds, it is characterized in that: before and after just spending, in time pull out the educated strain in the female parent, requirement pull out can educate strain before every strain bloom and can not surpass 2-3, can educate the educated strain of not pulling out after strain finishes and can not surpass 1% of remaining maternal sterile strain sum pulling out.
5, the cabbage type rape recessive cytoblast sterile two according to claim 1 method that is the production of hybrid seeds, it is characterized in that: when parent and production of hybrid seeds results, must gather in respectively, pile up separately, threshing respectively, the wind of listing shines and preserves respectively according to the situation of the mark of sterile strain or this plantation of seed farm father and mother.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96102204A CN1095329C (en) | 1996-01-22 | 1996-01-22 | Method for plantation of rape seeds of two lines with recessive nuclear sterility |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96102204A CN1095329C (en) | 1996-01-22 | 1996-01-22 | Method for plantation of rape seeds of two lines with recessive nuclear sterility |
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| Publication Number | Publication Date |
|---|---|
| CN1155373A CN1155373A (en) | 1997-07-30 |
| CN1095329C true CN1095329C (en) | 2002-12-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN96102204A Expired - Fee Related CN1095329C (en) | 1996-01-22 | 1996-01-22 | Method for plantation of rape seeds of two lines with recessive nuclear sterility |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094308C (en) * | 2000-03-27 | 2002-11-20 | 绵阳市农业科学研究所 | Method for discriminating male fertile plant in cross breeding of cabbage-type rape |
| CN102742496B (en) * | 2012-03-27 | 2014-08-06 | 贵州省油菜研究所 | Rapid transfer improvement method for rape recessive homozygosis sterile line |
| CN103718945A (en) * | 2012-10-11 | 2014-04-16 | 仲衍种业股份有限公司 | Nucleic male sterility two lines rape breeding method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091582A (en) * | 1993-02-23 | 1994-09-07 | 陕西省农垦科技教育中心 | Sulphur rape three-way crossbreeding technology in the Wild cabbage type low mustard |
| CN1098247A (en) * | 1993-08-05 | 1995-02-08 | 华中农业大学 | Rape cell nuclear+cytoplasm male sterility three line selection and breeding method |
| CN1104418A (en) * | 1994-02-21 | 1995-07-05 | 江苏省农业科学院 | Breeding method for capitata double hybrid rape |
| CN1125504A (en) * | 1994-12-28 | 1996-07-03 | 华中农业大学 | Hybrid seeding method of high-yield, high-quality and wild cabbage type rape |
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1996
- 1996-01-22 CN CN96102204A patent/CN1095329C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091582A (en) * | 1993-02-23 | 1994-09-07 | 陕西省农垦科技教育中心 | Sulphur rape three-way crossbreeding technology in the Wild cabbage type low mustard |
| CN1098247A (en) * | 1993-08-05 | 1995-02-08 | 华中农业大学 | Rape cell nuclear+cytoplasm male sterility three line selection and breeding method |
| CN1104418A (en) * | 1994-02-21 | 1995-07-05 | 江苏省农业科学院 | Breeding method for capitata double hybrid rape |
| CN1125504A (en) * | 1994-12-28 | 1996-07-03 | 华中农业大学 | Hybrid seeding method of high-yield, high-quality and wild cabbage type rape |
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| CN1155373A (en) | 1997-07-30 |
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