CN1125504A - Hybrid seeding method of high-yield, high-quality and wild cabbage type rape - Google Patents
Hybrid seeding method of high-yield, high-quality and wild cabbage type rape Download PDFInfo
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- CN1125504A CN1125504A CN 94119115 CN94119115A CN1125504A CN 1125504 A CN1125504 A CN 1125504A CN 94119115 CN94119115 CN 94119115 CN 94119115 A CN94119115 A CN 94119115A CN 1125504 A CN1125504 A CN 1125504A
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Abstract
The three-line cross breeding method for high-yield quality wild cabbage type rape features that a sterile line is cultured from "Bolima" cytoplasmic sterile line 114/A as female parent and maintainer line 1141B as male parent, and the "Huaza No.3" is cultured from sterile line 1141A as femal parent and restoring line 5200 as male parent, having advantages of high yield, and low contents of erucic acid and thioglucoside.
Description
The invention belongs to the selection of crop new varieties, particularly a kind of method of breeding high-yield high-quality cabbage type rape new varieties.
High yield, high-quality are the main targets of the present rapeseed breeding of China, also are the principal contradictions of China's rapeseed breeding.China carries out the quality rape breeding from early eighties, up to the present, although selected an endorsement low (low erucic acid), two low (low erucic acid, low sulfatide) rape variety, its yield potentiality still is lower than routine " two high " (high erucic acid, the high-sulfur glucoside) kind that large tracts of land is promoted in the present production.Some breeding units of China are utilized rape heterosis, selected several " two high " Hybrid, (producing method for seed of this kind has obtained Chinese invention patent to " No. two, Qin's oil " that cultivate as Educational Center of Agriculural-Settlement Science and Technology, Shaanxi Pro, the patent No. is 86108723.2), though these Hybrids have surpassed conventional two high kind on yield potentiality, but its quality still belongs to high erucic acid, high-sulfur glucoside, the current promotion rapeseed breeding of incompatibility high yield, high-quality direction do not meet current people yet and pursue diet to natural, health developing direction.The actual conditions that current China rape variety faces are, the quality rape kind is high yield not, and the rape variety of high yield high-quality not, therefore, how to overcome high-quality and high yield contradiction, adopting a kind of new selection to cultivate the good quality and high output new rape variety is that we face a challenge.
The producing method for seed that task of the present invention provides a kind of good quality and high output cabbage type rape three line hybrid seed utilizes hybrid vigour, selects erucic acid and glucosinolate content is low, the crossbreed that output is high.
The present invention is achieved through the following technical solutions:
The method of the cabbage type rape hybrid new varieties of a kind of seed selection high-quality, high yield, its key is: adopting agate (Polima) cytoplasmic male sterile line 1411A of Pori and recovery is 5200 to hybridize the generation that hybridizes, the seed selection and the cultivation method of the cabbage type rape first generation of hybrid are similar aspect much with at present for parent propagation of this hybrid and the seeding technique of hybrid, it adopts following program to obtain
(1) three series mating:
(1) parent's maintenance line (1141B): adopt maintenance line 75-3B to do maternal and two low kind Regent (male parent) hybridization, the F1 that obtains with hybridization does maternal and Japanese rape hybridization and obtains the F1 that reestablishes diplomatic relations again, make the continuous selfing of the F1 that reestablishes diplomatic relations again, its erucic acid and glucosinolate content are measured in plant division in its self progeny, and the while is continuous and the agate male sterile line 1238A of Pori backcrosses, filter out therein and have low erucic acid and low sulfatide feature, and can better keep the maintenance line 1141B of Pori's agate male sterility.
(2) parent's male sterile line: with 1238A is maternal, is paternal hybrid and continuous backcross with 1141B, obtains the assorted No. three parent's male sterile line 1141A of China.
(3) parent recover be: by (7211 * Marnoo) * ((S
75-1* DSV-SR-50) * S
75-1) obtain.
(2) parent's feature:
(1) male sterile line 1141A: the leaf look grayish green, and blade is bigger, weak spring habit, and the male sterile line petal is less, yellow, flowering is not smooth, and flower pesticide is degenerated, and gynoecium is grown normal, and content of erucic acid is lower than 1%, and sulphur glucoside total content is lower than 31 μ mol/g.
(2) maintenance line 1411B: its plant, leaf morphology are all identical with 1411A with quality trait, and in addition, its stamen development is normal, and petal is bigger, yellow, and open and flat.
(3) recovering is 5200: belong to semiwinterness, and dark green leaf color, blade is less, incises deeplyer, and branch is many and the branch position is low, and female stamen is grown normal, and petal is normally open and flat, yellow, the pollen abundance, content of erucic acid is lower than 1%, and sulphur glucoside total content is lower than 30 μ mol/g.
(4) hybrid Huaza No.3: belong to semiwinterness, dark green leaf color, blade is bigger, complete stool angle fruit number and every angle fruit grain number are more, thousand kernel weight is bigger, participates in provincial regional experiment of variety in continuous 3 years according to 1992-1994, respectively than " two high " check variety " middle oily 821 " volume increase 11.57%; 5.1% and 7.8%; Average volume increase 7% reaches utmost point significance level.1993 and 1994 participated in provincial kind zone in continuous 2 years, in the multiple spot regional test of ten points, respectively than " middle oily 821 " volume increase 12.56% and 11.33%, its content of erucic acid about 1%, glucosinolate content 31 μ mol/g, this kind precocity, average breeding time was than " middle oily 821 " precocious 1.6 days.The suitable planting scope is wide.The more anti-stalk break of this kind, virus disease, cold resistance and lodging resistance are medium.
According to above inventive concept, parent propagation in will inventing now and hybrid seeds technology also are described below in conjunction with the embodiments:
(1) parent propagation technical essential:
(1) male sterile line is 2: 1 with the capable ratio of maintenance line, line-spacing 33cm, spacing in the rows 19cm, every mu of about 10,000 strains;
(2) suitable late sowing is sowed about the Hubei Wuhan Area was with September 25, is grown seedlings seedling age 35～40 days;
(3) brassicaceous vegetable seed-plots such as isolated area requirement and other rape variety and Chinese cabbage, brassica campestris var purpurea, leaf mustard are isolated more than 1500 meters.Isolated area should select do not plant rape and planted the plot (preferably paddy field) that vegetables are reserved seed for planting in 2～3 years;
10 o'clock～11 o'clock every morning was carried out artificial supplementary pollination when (4) blooming;
(5) carry out repeatedly removal of impurities in seedling stage, elongation, florescence (emphasis), maturing stage according to male sterile line and maintenance line feature;
(6) maintenance line spends the back to require maintenance line is all pulled out eventually;
(7) except that applied nitrogen, should execute borax 0.5kg for every mu, superphosphate 35～40kg, but nitrogen application is too much unsuitable.
(2) hybrid seeds technical essential:
(1) male sterile line is that row is than being 2: 1 or 3: 2 or 4: 2 line-spacing 33cm, spacing in the rows 19cm, every mu of about 10,000 strains with recovering;
(2) suitable late sowing, as in the Hubei Wuhan Area, this is advisable father and mother with sowing about September 25;
(3) isolated area condition and parent propagation are basic identical, but isolation distance can shorten, but should not be less than 1000 meters, and isolated area is if the nonirrigated farmland, then require to select not plant in 2～3 years the field piece that rape or brassicaceous vegetable are reserved seed for planting;
(4) 10～11 o'clock every mornings of flowering stage carry out artificial supplementary pollination or put honeybee pollination;
(5) bloom earlier because of female parent, be placed on and bloom preceding about 10 days, pluck the maternal main tongue of row and 1～2 branch, postponed about a week maternal flowering stage, can guarantee this flower synchronization of father and mother;
(6) seedling stage, elongation, florescence (emphasis) and maturing stage according to male sterile line, convalescence feature carry out the multiple spot removal of impurities;
(7) fertilization type and Fertilization Level are identical with the parent propagation technology.
Good effect of the present invention:
According to Inst. of Oil Crops, Chinese Academy of Agriculture (oily institute in the abbreviation) the arrangement 1992-1994 year quality rape regional test result of Hubei Province, the rape first generation of hybrid kind that method of the present invention is cultivated " assorted No. three of China " and check variety " middle oily 821 " and other 8 strain (kind) breedinesses and quality trait thereof altogether are as shown in table 1:
Table 1: 8 strain breedinesses such as Huaza No.3 and middle oil " 821 " and quality trait are relatively
| Family name | The source | Characteristic | Quality erucic acid % | Proterties sulphur glucoside μ mol/g | The unit of providing |
| Huaza No.3 7,208 950 737 | 1141A * recovery be assorted No. three 2208A * recovery of 90-China be No. 1 China's oil hang down mustard 821 *, (magnificent oily No. 3 * Marnoo), (82-5 * 8082-1) * Bakow | The two low yellow seeds of the two low male sterile line hybrids of two low male sterile line hybrids are two low | 1.86 1.32 2.47 1.42 0.131 1.01 2.4 0.89 | ?31.57 ?31.07 ?54.84 ?32.19 ?12.4 ?28.31 ?22.5 ?32.11 | Central China agricultural university of Central China agricultural university of Central China agricultural university of Central China agricultural university of oily institute in the oil institute in Central China agricultural university of Central China agricultural university |
| Oil 821 among 86002 90-16 89008 | Bakow * middle oily 821 8-03 * unified the contrast of middle oily 821 84004 * Ruo | Two low two low two low two high | ?0.876 ?0.79 ?0.653 ?0.83 ?0.882 ?0.65 ?46.26 ?48.39 | ?9.46 ?21.17 ?8.15 ?19.43 ?9.98 ?23.23 ?105.17 ?123.46 | In oily institute in the oil institute in the oil institute in the oil institute in the oil institute in the oil institute in the oil institute in the oil institute |
Find out from table 1 quality trait test result, 1992～1993 years two low strains except that the glucosinolate content of 7208 strains is higher, all the other participate in the experiment strain erucic acid and glucosinolate contents all meet two low quality rape standards.
Eight homologous pints product owners of table 2 are imitated multiple ratio
| Strain | Average yield per mu kg/ mu | Difference 5% | Significance 1% | Comparison photograph ± % |
| Oil 821 950 7,208 86002 90-16 89,006 737 in the Huaza No.3 | ????156.8 ????145.4 ????140.0 ????131.5 ????130.3 ????123.0 ????111.2 ????107.7 | ????a ????b ????c ????d ????d ????e ????f ????f | ????A ????B ????B ????C ????C ????D ????E ????E | ????+7.8 ????-3.7 ????-9.6 ????-10.4 ????-15.4 ????-23.5 ????-25.9 |
The result finds out from table 2, and the output of eight homologous pintses is the highest with the assorted No. three strain output of China, average specific contrast (middle oily 821) volume increase 7.8%, and volume increase reaches extremely remarkable.Comparison is according to underproduction 3.7-25.9% respectively in all the other test sample systems, and the underproduction reaches significantly and be extremely remarkable.
" assorted No. three of China " by the agriculture and animal husbandry Room, Hubei Province tissue gathers as following table in Hubei the whole province multiple spot result of the test
Table 3: China mixes No. three in Hubei Province's multiple spot test output situation
| The place | 1992-1993 kg/ mu | ±％ | 1993-1994 kg/ mu | ±％ | ||
| Middle oil 821 (ck) | Assorted No. three of China | Middle oil 821 (ck) | Assorted No. three of China | |||
| Farming section center Chongyang bureau of agriculture of bureau of agriculture Qianjiang City, Jianglin County bureau of agriculture Chongyang, Jingmen bureau of agriculture Zhijiang county Lichuan City bureau of agriculture of bureau of agriculture Xishui farming section center thing lake, Hua Zhong Agriculture University Xiangfan City institute of agricultural sciences Xiaogan City is average | ????82.99 ????188.5 ????78.5 ????178.4 ????167.0 ????116.5 ????136.75 ????184.2 ????127.2 ????138.05 | ?111.91 ?211.7 ?132.5 ?187.5 ?170.25 ?169.0 ?152.4 ?205.0 ?143.1 ?158.54 | +34.8 +12.3 +68.8 +5.1 +1.9 +45.1 +11.4 +11.3 +12.5 +14.84 | ????99.2 ????207.5 ????141.0 ????141.0 ????163.9 ????158.3 ????102.5 ????195.0 ????151.05 | ?105.0 ?255.8 ?170.0 ?162.5 ?171.6 ?174.7 ?99.25 ?206.5 ?168.17 | +5.85 +23.28 +20.57 +15.25 +4.70 +10.36 -3.22 +5.89 +11.33 |
The unified design of ※ test, randomized arrangement, three repetitions, 40 square metres of sub-district areas.
Claims (3)
1, a kind of method of cabbage type rape cytoplasmic male sterilty three-way cross hybrid use of advantage, this method comprises safe isolation, proper seeding, rational close planting, suitable row ratio, regulate the florescence, artificial supplementary pollination, roguing operating procedure, it is characterized in that, the agate cytoplasmic sterile line 1141A of seed selection Pori does maternal, and maintenance line 1141B makes paternal hybrid, breeding 1141A male sterile line; It is maternal that male sterile line 1141A does, and recovery is 5200 to make paternal hybrid preparation low erucic acid, low sulfatide Huaza No.3 first-filial generation kind.
2, the method for claim 1 is characterized in that, this suitable row of father and mother is than being 1: 2 during parent propagation.
3, the method for claim 1 is characterized in that, this suitable row of father and mother is than being 1: 2 or 2: 3 or 2: 4 during the production of hybrid seeds of first-filial generation kind.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94119115A CN1042188C (en) | 1994-12-28 | 1994-12-28 | Hybrid seeding method of high-yield, high-quality and wild cabbage type rape |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94119115A CN1042188C (en) | 1994-12-28 | 1994-12-28 | Hybrid seeding method of high-yield, high-quality and wild cabbage type rape |
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| Publication Number | Publication Date |
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| CN1125504A true CN1125504A (en) | 1996-07-03 |
| CN1042188C CN1042188C (en) | 1999-02-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN94119115A Expired - Fee Related CN1042188C (en) | 1994-12-28 | 1994-12-28 | Hybrid seeding method of high-yield, high-quality and wild cabbage type rape |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095329C (en) * | 1996-01-22 | 2002-12-04 | 贵州省油料科学研究所 | Method for plantation of rape seeds of two lines with recessive nuclear sterility |
| CN106069013A (en) * | 2016-06-15 | 2016-11-09 | 上海交通大学 | The purple rapeseed cultivation method controlled based on the temperature difference |
| CN106538180A (en) * | 2016-10-11 | 2017-03-29 | 贵州省油菜研究所 | A kind of method for improving cabbage type rape yield |
| CN111758554A (en) * | 2020-07-14 | 2020-10-13 | 江西省农业科学院作物研究所 | Breeding method of cabbage type early-maturing rape variety |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1019756B (en) * | 1988-10-10 | 1992-12-30 | 高波 | Mold body modelling method |
| CN1018703B (en) * | 1989-12-19 | 1992-10-21 | 上海市农业科学院作物育种栽培研究所 | Wild cabbage type rape kernet malesterile three-line seed technology |
| CN1034848C (en) * | 1993-02-23 | 1997-05-14 | 陕西省农垦科技教育中心 | Wild cabbage type low mustard, middle sulphur rape three-way crossbreeding technology |
-
1994
- 1994-12-28 CN CN94119115A patent/CN1042188C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095329C (en) * | 1996-01-22 | 2002-12-04 | 贵州省油料科学研究所 | Method for plantation of rape seeds of two lines with recessive nuclear sterility |
| CN106069013A (en) * | 2016-06-15 | 2016-11-09 | 上海交通大学 | The purple rapeseed cultivation method controlled based on the temperature difference |
| CN106069013B (en) * | 2016-06-15 | 2020-01-07 | 上海交通大学 | Method for planting brassica campestris based on temperature difference control |
| CN106538180A (en) * | 2016-10-11 | 2017-03-29 | 贵州省油菜研究所 | A kind of method for improving cabbage type rape yield |
| CN111758554A (en) * | 2020-07-14 | 2020-10-13 | 江西省农业科学院作物研究所 | Breeding method of cabbage type early-maturing rape variety |
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| CN1042188C (en) | 1999-02-24 |
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