CN1250071C - Breeding of cabbage type rape micropowder-free cytoplasmic male sterile line and hybrid seed production method - Google Patents
Breeding of cabbage type rape micropowder-free cytoplasmic male sterile line and hybrid seed production method Download PDFInfo
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- CN1250071C CN1250071C CN 200410025802 CN200410025802A CN1250071C CN 1250071 C CN1250071 C CN 1250071C CN 200410025802 CN200410025802 CN 200410025802 CN 200410025802 A CN200410025802 A CN 200410025802A CN 1250071 C CN1250071 C CN 1250071C
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Abstract
The invention discloses a method for breeding a micropowder-free cytoplasmic male sterile line of brassica napus and a method for producing hybrid seeds. Adopting the rape Ledos and the double No. 2 inbred line in the Chinese rape variety to carry out cross breeding on a sterile line, and carrying out backcross on the double No. 2 inbred line in the Chinese rape to breed a micropowder-free double-low sterile line and a corresponding maintainer line; adopting compound hybridization to select restoring line; the combining ability analysis is carried out by a double-row hybridization method, and combinations are selected from the combining ability analysis and used for producing hybrid seeds. Compared with the prior art, the invention can reduce the production risk of hybrid seeds and improve the proportion of heterozygous genotypes in the hybrid seeds. The invention successfully breeds No. 6 Shaanxi and No. 8 Shaanxi, and increases the rape varieties with large yield-increasing potential and wide adaptability for the rape.
Description
Technical field
The rape heterosis that the invention belongs to agricultural utilizes technology, relates to seed selection and producing method of hybrid that a kind of cabbage type rape does not have micro mist cell type matter male sterile line.
Background technology
The heterosis utilization of rape has number of ways, mainly comprises cytoplasmic sterility series hybrid, the sterility cross-bred kind of cell nucleus, changes and kill crossbreed and self incompatible line hybrid.Mainly be that to utilize cytoplasmic sterility three be that (being male sterile line, maintenance line, recovery system) produces crossbreed at present.The rape cytoplasmic male sterile line of finding mainly contains Polima CMS, Shan 2A, Ogu CMS, nap CMS, nig CMS, mur CMS, MI CMS, Nca and RM5637 both at home and abroad.Polima CMS is that Fu Tingdong in 1972 teaches the natural male sterile plant of finding in Polish cabbage type rape Polima, can be divided into the sterile type of high temperature, the sterile type of low temperature and stable type by its performance, and the male sterile line of stable type accounts for 8.5%.Research thinks that the performance of Polima male sterile line is relevant with maintenance line and environment, can breed stable cytoplasmic male sterile line by a large amount of screening maintenance lines.Shan 2A is the male sterile material that Li Dianrong finds in combination is reestablished diplomatic relations in cabbage type rape variety S74-3 * (good harvest No. 4+7202), the male sterile line Shan 2A that breeds in nineteen eighty-two, and its fertility performance is also relevant with external environment.Ogu CMS is the natural male sterile plant that nineteen sixty-eight Ogura finds from radish colony at Japan Kagoshima, this sterile material transformation in the cabbage type rape after, the male sterile line fertility is very stable, but use also existing problems, the blade yellow when mainly being low temperature, nectary development is bad and lack it in cabbage type rape recovers system.Nap CMS is that Thompson in 1972 makes the F that male parent and winter rape variety RD58 etc. make hybridization of female parent from Polish spring rape kind Bronowsiki
2The cytoplasm male sterility line of finding in generation, the fertility instability of this sterile material, very responsive to temperature, when temperature was higher than 20 ℃, pollinia appearred.Nig CMS is that Peason in 1972 is the F of black mustard and wild cabbage
1Handle with colchicin for plant, make chromosome doubling, the pollen with broccoli carries out continuous backcross again, the cytoplasmic male sterile line of seed selection, and this male sterile line lacks maintenance line.Mur CMS is after Hinata in 1979 and Konno carry out chromosome doubling with the first generation of hybrid of Chinese cabbage type kind Yukin with colchicin to D.muralis (Cruciferae), the turnip type rape male sterile line of breeding with the Yukin continuous backcross again, this male sterile line will be used, need better maintenance line, and maintenance line relates to the self-incompatibillity problem, so the breeding of maintenance line is very difficult.Among cytoplasmic male sterile line MI CMS, the Nca of domestic new seed selection and RM5637 are studying.
In sum, in the male sterile line of finding, the sterility of Ogu CMS is very stable, and the production utilization of mur CMS and nig CMS male sterile line also has some problems anxious to be solved.Polima, nap, the fertility of male sterile lines such as Shan 2A is all relevant with maintenance line and external environment, and specification requirement is very strict in these cytoplasmic male sterile line production of hybrid seeds processes.Therefore, need seed selection not have micro mist cell type matter male sterile line in the production, improve seed production purity.
Summary of the invention
The objective of the invention is to, provide a kind of cabbage type rape not have the seed selection and the producing method of hybrid of micro mist cell type matter male sterile line.
This method is realized by following technical scheme: adopt colea and china rape to carry out the selection cross male sterile line, and keep with the male sterile line of china rape to seed selection; Adopt the multiple cross seed selection to recover system; Hybrid combination screening and crossbreed produce.May further comprise the steps:
1) seed selection of male sterile line and maintenance line
With German double-low rapeseed kind ledos is maternal, does hybridization, seed selection sterile cytoplasm from its filial generation for male parent two No. 2 in the Chinese Double-low Rapeseed kind.Select for use selfing for many years in two No. 2 inbred line S212 it is kept, 6 generations of continuous backcross, breed two low male sterile line 212A of stable type and corresponding maintenance line 212B.
2) seed selection of recovery system
Utilize the multi-parent strain multiple cross, make excellent genes produce polymerization, seed selection recovers system: 220/3559 hybrid combination of assembly Henan, No. 3/Ledos of Qin's oil hybrid combination obtains two F
1, use two F again
1Hybridize the combination that obtains reestablishing diplomatic relations, carry out systematic breeding among the combination offspring that from then on reestablishes diplomatic relations, successively select 116C and 1102C.
4) breeding of hybrid combination
Adopt the diallel cross method to carry out Analysis of Combining Ability, therefrom filter out combination 212A/116C and 212A/1102C and be used for crossbreed production;
5) crossbreed produces
1. production of hybrid seeds district: utilize 212A to produce crossbreed.The suitable production of hybrid seeds zone of this male sterile line is Shan Nan, can overcome this male sterile line in the positive season sowing of Shan Nan and just spend dead flower bud phenomenon, improves hybrid seed yield and quality greatly.
2. isolate: adopt distance isolation, it is that 1000m is with interior other rape variety or the crop in cruciferae of not planting that isolated area requires;
3. proper seeding: comprise whole ground, grow seedlings, seedbed management and transplanting;
4. row compares: father and mother's one's own profession ratio is 1: 2;
5. density: mu stays about seedling 8000 strains, the 2/3 maternal 212A that plants, 1/3 plant male parent 116C or 1102C;
6. impurity elimination: the assorted strain in rape seedling and initial bloom stage removal father and mother one's own profession, educated strain, variant, sick and weak strain in the promptly maternal row, variant, sick and weak strain in the male parent row;
7. surgery male parent: the seed farm rape is surgeried male parent 116C or 1102C after spending eventually.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
1. the seed selection of male sterile line:
With ledos (Germany, two low) is maternal, in two No. 2 (China, two low) do hybridization for male parent.In the progeny population of this combination, find 5 strain male sterile plants, the petal of this sterile material is little, stamen is brown, similar cell nucleus is sterile, its maximum characteristics are the initial bloom stage complete stool dead flower bud phenomenon to be arranged, main inflorescence is the most obvious, but the colored sterility that the later stage opens is very thorough, manually dials the flower bud selfing and does not have solid.Material with separate sources keeps its sterility, the result shows the sterility of the maintenance 212A that the maintenance line of Polima, shaan 2a cms all can be good, the male sterile line of all transformations does not all have trace-pollen and produces, and these male sterile lines all have the dead flower bud phenomenon of initial bloom stage.Selecting strong, the colory male sterile line 212A of growth potential to be used for the hybrid combination survey from the male sterile line of transformation joins.This male sterile line content of erucic acid 0.63%, glucosinolate content 16-20 μ mol/g.
2. the seed selection of maintenance line:
To under northern ecotope, taming for two No. 2 in the semiwinterness material of the Yangtze river basin, select growth potential strong, the cold-resistant continuous selfed breeding of individual plant becomes S212, with it 212A is kept, and has selected the two low maintenance line S212 of 212A, called after 212B.
3. recovering is the seed selection of 116C and 1102C:
Utilize the multi-parent strain multiple cross, make excellent genes produce polymerization, seed selection recovers system: assembly Henan 220 (Henan agricultural science, the fifth phase in 1991, two low)/3559 (this seminar seed selections, low mustard, disease resistance is strong) hybrid combination, the Qin oil No. 3 (Shaanxi agricultural, 1989, low erucic acid recovers system)/Ledos hybrid combination, obtain two F
1, use two F again
1Hybridize the combination that obtains reestablishing diplomatic relations.From then on reestablish diplomatic relations to make up among the offspring and carry out systematic breeding, successively select 116C (low erucic acid) and 1102C (two low).
4. breeding of hybrid combination:
Adopt the diallel cross method, it is maternal hanging down sterile with 4 pairs, and 4 recoveries are male parent, are mixed with 16 hybrid combinations and carry out Analysis of Combining Ability, and the result shows that the economic characters that make up 212A/116C are outstanding, and comparison is shone material and increased production 8.5%.Use that to measure the advantage of 212A/1102C with quadrat method strong next year, comparison is according to material volume increase 9.5%.Respectively attend Shaanxi Province's rape regional test and national rape regional test with these two combinations, the back is evenly by authorization.Variety name is No. 6, Shan oil (economize and examine) and No. 8, Shan oil (state examines).
No. 6, Shan oil, semiwinterness, about 252 days breeding times, seedling stage half is upright, leaf look pale green, the non-bolter winter before, the back poor growth of turning green is just spent the back growth rapidly, the florescence concentrates, maturing stage and the Qin oily No. 2 suitable.Content of erucic acid 0.61%, glucosinolate content 60-70 μ mol/g.Suit in Guanzhong, Shan Nan and the plantation of similar area.
No. 8, Shan oil, semiwinterness, about 250 days breeding times, seedling stage half is upright, leaf look pale green, greenery are several more than 12 before surviving the winter, and are difficult for bolting before the winter, and it is slow to first flower growth to turn green, and just spends the back growth rapidly, and the florescence concentrates, and seed phase filling speed is fast.Plant height 170-190cm, the effective branch amount 9-10 of individual plant, several about 400 of complete stool effective angle fruit, 26 of seed number per pods are about thousand kernel weight 3.5 grams.Viral diseases, anti-stalk break.Quality is measured content of erucic acid 0.28%, glucosinolate content 25.71umol/g, oil content 41.04% through Ministry of Agriculture's oil plant and quality of item supervision and inspection center (Wuhan).No. 8, Shan oil is in the examination of country district, and 2098.5 kilograms of two annual per hectares are than check variety volume increase 5.5%; 2778.0 kilograms of the average per hectares of production test are than check variety volume increase 5.74%.Through in the Hanzhong, the Chuzhou, Anhui, to the east of, Anshun, Guizhou, ground demonstrations such as Cheng County, Gansu, Yancheng, Jiangsu more than 15%, are that a yield potential is big, the rape variety of wide adaptability than local main breed volume increase all.Suit at Guanzhong, Shan Nan the plantation of ground such as Henan, Anhui, Jiangsu.
5. production of hybrid seeds zone is selected:
212A is carried out production of hybrid seeds test in Shan Nan, Gansu, Qinghai, the result shows in the positive season production of hybrid seeds of Shan southing row, can overcome the dead flower bud phenomenon of 212A male sterile line initial bloom stage, does not produce dead flower bud, and all buds are the energy normal crack all, and sterility is thorough.The south production of hybrid seeds can improve hybrid seed yield greatly in Shan, reduces production costs.
6. sterile line propagation:
1. breed environment: in Cheng County, Gansu breeding, adopt distance isolation, require 1500 meters of isolated areas can not plant other rape variety and crop in cruciferae, root out, guarantee isolation safe as finding should give with interior.The multiplication plot should select 2-3 not plant the field piece of rape or crop in cruciferae.
2. date of seeding: in the normal autumn sowing in Cheng County, Gansu, date of seeding is mid-September.
3. density: male sterile line 212A mu stays seedling 6000 strains, and 212B mu stays seedling 3000 strains.
4. row compares: 212A and 212B went between ratio than planting by 2: 1.
5. roguing: at rape seedling and initial bloom stage that the assorted strain in father and mother's one's own profession, lopsided strain, short change strain, the disposable removal of sick and weak strain is clean, guarantee parent propagation purity.
6. surgery male parent: the seed farm rape is surgeried male parent 212B after spending eventually.
7. crossbreed produces:
1. production of hybrid seeds district: the suitable zone that utilizes 212A to produce crossbreed is Shan Nan.
2. isolate: adopt distance isolation, it is that 1000m is with interior other rape variety or the crop in cruciferae of not planting that isolated area requires.
3. proper seeding: major technique comprises whole ground, grows seedlings, seedbed management and transplanting.
Meticulous whole ground uses sufficient base fertilizer before growing seedlings.Mu is executed 15 kilograms of diammonium phosphate, and 15 kilograms in urea is handled soil for 4 jin/mu with first enemy powder before broadcasting; South, Shan begins to grow seedlings mid-September, guarantees full stand and healthy seedling; Emerging, the back opposes powder with first or every mu of 2-3 jin of 1605 powder is prevented and treated phyllotreta striolata.Seedling during the 2-3 leaf, the phase final singling of 3-4 leaf, 5 leaf phases applied night soil in right amount; Played transplantation of seedlings in seedling age 35-40 days, pouring water in right amount before the transplanting guarantees that root system is complete.Remove variant or assorted strain before the transplanting, in 10 months, transplant the last ten-days period, big transplantation of seedlings is in time poured water after the transplanting.
4. row ratio: father and mother's one's own profession ratio is 1: 2, i.e. 1 row male parent, and 2 row are maternal.
5. density: mu stays about seedling 8000 strains, the 2/3 maternal 212A that plants, 1/3 plant male parent 116C or 1102C.
6. impurity elimination: the assorted strain in rape seedling and initial bloom stage removal father and mother one's own profession, educated strain, variant, sick and weak strain in the promptly maternal row, variant, sick and weak strain in the male parent row.
7. surgery male parent: the seed farm rape is surgeried male parent 116C or 1102C after spending eventually.
Claims (1)
1. a cabbage type rape does not have the seed selection of micro mist cell type matter male sterile line and the production method of crossbreed, it is characterized in that, adopts colea and china rape to carry out the selection cross male sterile line, and with china rape male sterile line is kept; Adopt the multiple cross seed selection to recover system; Hybrid combination screening and crossbreed produce; May further comprise the steps:
1) seed selection of male sterile line
With the two low cabbage type rape variety ledos of Germany is maternal, two No. 2 is paternal hybrid in the Chinese Double-low Rapeseed kind, the sterile strain that filial generation is occurred keeps with two No. 2 inbred lines in the selfing for many years, 6 generations of continuous backcross, breeds the two low male sterile lines of no micro mist type;
2) seed selection of maintenance line
To under northern ecotope, taming for two No. 2 in the semiwinterness material of the Yangtze river basin, select growth potential strong, the cold-resistant continuous selfing of individual plant, seed selection becomes the maintenance line of the two low male sterile lines of no micro mist type, with this maintenance line the two low male sterile lines of no micro mist type is kept;
3) seed selection of recovery system
Utilize the multi-parent strain multiple cross, make excellent genes produce polymerization: 220/3559 hybrid combination of assembly Henan, No. 3/Ledos of Qin's oil hybrid combination obtains two F
1, use two F again
1Hybridize the combination that obtains reestablishing diplomatic relations, carry out systematic breeding in the combination progeny material of from then on reestablishing diplomatic relations, select the recovery system of the two low male sterile lines of no micro mist type respectively;
4) breeding of hybrid combination
Adopt the diallel cross method to carry out Analysis of Combining Ability, therefrom filter out two low male sterile lines of no micro mist type and the combined material that recovers system, be used for crossbreed and produce;
5) crossbreed produces
1. production of hybrid seeds district: the suitable zone that utilizes the two low male sterile lines of no micro mist type to produce crossbreed is Shan Nan;
2. isolate: adopt distance isolation, it is that 1000m is with interior other rape variety or the crop in cruciferae of not planting that isolated area requires;
3. proper seeding: comprise whole ground, grow seedlings, seedbed management and transplanting;
4. row compares: father and mother's one's own profession ratio is 1: 2, and male parent is selected the recovery system of the two low male sterile lines of no micro mist type for use, and female parent is selected the two low male sterile lines of no micro mist type for use;
5. density: mu stays about seedling 8000 strains, 2/3 female parent of planting, 1/3 male parent of planting;
6. impurity elimination: the assorted strain in rape seedling and initial bloom stage removal father and mother one's own profession, educated strain, variant, sick and weak strain in the promptly maternal row, variant, sick and weak strain in the male parent row;
7. surgery male parent: the seed farm rape is surgeried male parent after spending eventually.
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| CN 200410025802 CN1250071C (en) | 2004-01-05 | 2004-01-05 | Breeding of cabbage type rape micropowder-free cytoplasmic male sterile line and hybrid seed production method |
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| CN 200410025802 CN1250071C (en) | 2004-01-05 | 2004-01-05 | Breeding of cabbage type rape micropowder-free cytoplasmic male sterile line and hybrid seed production method |
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| CN1250071C true CN1250071C (en) | 2006-04-12 |
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| CN 200410025802 Expired - Fee Related CN1250071C (en) | 2004-01-05 | 2004-01-05 | Breeding of cabbage type rape micropowder-free cytoplasmic male sterile line and hybrid seed production method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102224801B (en) * | 2011-04-19 | 2012-09-26 | 江苏省农业科学院 | Rapid multi-target property polymerization breeding method for rape |
| CN102696474B (en) * | 2012-05-15 | 2013-11-06 | 西北农林科技大学 | Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish |
| CN108739327B (en) * | 2018-06-19 | 2020-05-29 | 安徽省农业科学院园艺研究所 | Seed production method of capsicum annuum nuclear male sterile dual-purpose line |
| CN108925422A (en) * | 2018-07-27 | 2018-12-04 | 西南大学 | Improve the cytoplasmic male sterility Hybrid preparation method of rapeseed cultivation purity |
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