CN106069013A - The purple rapeseed cultivation method controlled based on the temperature difference - Google Patents

The purple rapeseed cultivation method controlled based on the temperature difference Download PDF

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CN106069013A
CN106069013A CN201610427137.4A CN201610427137A CN106069013A CN 106069013 A CN106069013 A CN 106069013A CN 201610427137 A CN201610427137 A CN 201610427137A CN 106069013 A CN106069013 A CN 106069013A
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temperature
purple
daytime
nocturnal
temperature difference
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CN106069013B (en
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唐东芹
赵洪
隽加香
黄丹枫
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Shanghai Jiaotong University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of purple rapeseed cultivation method controlled based on the temperature difference, purple Brassica campestris L is specially placed in temperature in daytime and grows under conditions of there is the temperature difference with nocturnal temperature by described method.Compared with prior art, present invention have an advantage that (1) present invention particularity based on purple Brassica campestris L, control to cultivate to its temperature difference, fill up the temperature difference and controlled to cultivate the blank of application in purple rapeseed cultivation technology, be that the field popularization and application in purple rapeseed cultivation of this technology provide theoretical basis simultaneously.(2) present invention has creatively screened and has been applicable to the purple temperature in daytime of growth of rape, the combination of nocturnal temperature, the purple morphological index of Brassica campestris L, yield index, physical signs etc. is made to have significantly optimization, the quality making purple Brassica campestris L is obviously improved, and such quality optimization degree is the temperature difference to control culture technique to be applied on other vegetable species institute inaccessiable.

Description

The purple rapeseed cultivation method controlled based on the temperature difference
Technical field
The present invention relates to a kind of purple rapeseed cultivation method controlled based on the temperature difference.
Background technology
Purple Brassica campestris L is the one of ornamental type vegetable, its resistance, and sight and edibility are high, the advantage such as can broadcast the anniversary. This kind is introduced from external, belongs to new varieties at home, and the research to its growth and cultivation is fewer at present, especially grows it The control of ambient temperature is almost without being involved.Within 1981, left literary composition of giving birth have studied Nanning City's high temperature shadow to Plantula Brassicae chinensis with Cai Shufa Sound draws under high temperature and the drying condition of more than 25 DEG C, and growing weak, plant is short and small, and yield is the lowest.It is the suitableeest that its young root grows Temperature is 26 DEG C, is up to 36 DEG C, minimum 4 DEG C.But its experiment is mainly carried out out of doors, related data is not had to make to join Examine, the most day and night temperature is not made any research.The more important thing is, purple Brassica campestris L [Brassica campestris L.ssp.chinensis (L.)] and Plantula Brassicae chinensis [(Brassica campestris L.ssp.chinensis Makino (var.communis Tsen et Lee] differ farther out in spore classification, and form, yield, physiology aspect are also deposited In significant difference, the temperature control technology on the vegetables such as Plantula Brassicae chinensis is very difficult to apply on purple Brassica campestris L so existing, causes purple Brassica campestris L Temperature control growing technology hindered.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide a kind of purple rapeseed cultivation side controlled based on the temperature difference Method.The present invention is the impact simulating different day and night temperatures in work climate box to purple growth of rape.The experimental data drawn The impact on purple Brassica campestris L of the more actual response day and night temperature.The comprehensive data analyzing gained, daytime, temperature was 20 DEG C, purple when night, temperature was 10 DEG C Brassica campestris L general performance is optimal.
The present invention is achieved by the following technical solutions:
The present invention provides a kind of purple rapeseed cultivation method controlled based on the temperature difference, and described method is specially and is placed in by purple Brassica campestris L Daytime, temperature grew under conditions of there is the temperature difference with nocturnal temperature.
Preferably, described daytime, temperature was in the range of 20-30 DEG C, and nocturnal temperature is in the range of 10-20 DEG C.
It is further preferred that being combined as of temperature in described daytime, nocturnal temperature: daytime temperature 30 DEG C, nocturnal temperature 20 DEG C (W1);Daytime temperature 25 DEG C, nocturnal temperature 15 DEG C (W2);Daytime temperature 20 DEG C, nocturnal temperature 10 DEG C (W3);Daytime temperature 30 DEG C, nocturnal temperature 15 DEG C (W4);Daytime temperature 25 DEG C, nocturnal temperature 10 DEG C (W5);Daytime temperature 30 DEG C, nocturnal temperature 10 DEG C (6)。
It is highly preferred that being combined as of temperature in described daytime, nocturnal temperature: daytime temperature 30 DEG C, nocturnal temperature 20 DEG C (W1); Daytime temperature 20 DEG C, nocturnal temperature 10 DEG C (W3);Daytime temperature 25 DEG C, nocturnal temperature 10 DEG C (W5).
It is particularly preferred that being combined as of temperature in described daytime, nocturnal temperature: daytime temperature 30 DEG C, nocturnal temperature 20 DEG C (W1);Daytime temperature 20 DEG C, nocturnal temperature 10 DEG C (W3).
Most preferably, temperature in described daytime, being combined as of nocturnal temperature: daytime temperature 20 DEG C, nocturnal temperature 10 DEG C (W3).
Preferably, described purple Brassica campestris L be SANYE wholeheartedly when transplanted in daytime temperature there is the temperature difference with nocturnal temperature Under conditions of growth.
Preferably, the ambient humidity of described growth is about 85%.
Preferably, in described growth course, every liter of substrate 4g of green slow-release fertilizer difficult to understand used by fertilizer.
Preferably, described growth is carried out in growth cabinet.
After the inventive method cultivates 30d continuously, purple Brassica campestris L just can grow to more than 10 leaves.
Compared with prior art, the present invention possesses following beneficial effect:
(1) present invention particularity based on purple Brassica campestris L, controls to cultivate to its temperature difference, has filled up the temperature difference and has controlled to cultivate at purple oil The blank of application in colza planting technology, is that the field popularization and application in purple rapeseed cultivation of this technology provide theoretical base simultaneously Plinth.
(2) present invention has creatively screened and has been applicable to the purple temperature in daytime of growth of rape, the combination of nocturnal temperature so that The purple physiological and biochemical index of Brassica campestris L, morphological index etc. are notable to be optimized, and makes the quality generation qualitative leap of purple Brassica campestris L, such quality Degree of optimization be the temperature difference is controlled culture technique be applied on other vegetable species institute inaccessiable.
Accompanying drawing explanation
By the detailed description non-limiting example made with reference to the following drawings of reading, the further feature of the present invention, Purpose and advantage will become more apparent upon:
Fig. 1 is purple Brassica campestris L water content testing result schematic diagram;
Fig. 2 is purple Brassica campestris L soluble protein testing result schematic diagram;
Fig. 3 is purple Brassica campestris L vitamin c testing result schematic diagram;
Fig. 4 is purple Chlorophyll in Rape testing result schematic diagram;
Fig. 5 is purple Brassica campestris L soluble sugar testing result schematic diagram;
Fig. 6 is purple Brassica campestris L anthocyanidin testing result schematic diagram;
Fig. 7 is purple Brassica campestris L nitrate nitrogen testing result schematic diagram;
Fig. 8 is purple Reducing Strength of Rape Roots testing result schematic diagram;
Fig. 9 is purple Brassica campestris L integral status picture;
Figure 10 is purple Brassica campestris L local blade picture.
Detailed description of the invention
Below in conjunction with instantiation, the present invention is described in detail.Following instance will assist in those skilled in the art It is further appreciated by the present invention, but limits the present invention the most in any form.It should be pointed out that, to those of ordinary skill in the art For, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into the guarantor of the present invention Protect scope.
1, materials and methods
Experiment place: growth cabinet;
Substrate: organic composite substratd (Huai'an Zhong Nuo development in agricultural science and technology company limited)+Osmocote (every liter of 4g)
Sowing: starting sowing on the 27th in December in 2015, nursery is organic composite substratd by 200 hole standard hole tray, substrate, Seedling stage does not applies fertilizer.
Transplanting: on February 1st, 2016, wholeheartedly (it is complete true that SANYE wholeheartedly refers to that existing three leaves grow up to substantially to plant SANYE Leaf, the 4th state that leaf germinates) time start to be transplanted in 32 hole standard hole tray, during transplanting, substrate is mixed into Osmocote As base manure, according to the ratio of every liter of 4g, Osmocote is uniformly mixed in substrate, it follows that sabot, transplants.The most slowly The hole tray transplanted is immersed in equipped with (about 3 liters of clear water of each pallet) in the pallet of clear water by ground, soaks about 30 minutes, substrate by the time Move on in tested growth cabinet together with pallet after being impregnated with water.
Cultivation management: moisture, substrate waters a water for 5 days.Air humidity is maintained at about 85%, no longer chases after during growth Fertile.
The index of result needs detection:
Physical signs: water content, anthocyanidin, vitamin c, nitrate nitrogen, soluble sugar, soluble protein, chlorophyll, root system Vigor.
Morphological index: plant height, Ye Kuan, leaf is long, leaf area, plant photo, single leaf photo.
Yield index: plant fresh weight, plant weights, aerial parts dry weight and fresh weight of plant seedlings, root dry weight and fresh weight of plant seedlings, root/shoot ratio.
Concrete grammar:
The purple Brassica campestris L difference differential temperature of table 1 processes (unit DEG C)
Index test: to said method plantation purple Brassica campestris L physical signs (anthocyanidin, Vc, nitrate nitrogen, soluble sugar, Chlorophyll, improving activity of root system), morphological index (leaf is long for plant height, Ye Kuan, whole strain, single leaf), yield index (aerial parts dry weight and fresh weight of plant seedlings, Under ground portion dry weight and fresh weight of plant seedlings, root/shoot ratio, water content) detect, wherein:
The detection method of anthocyanidin is: soda acid chromatism method measures (Lin Wenchao, 2011);
The detection method of Vc is: xylene extraction colorimetric method for determining (Li Hesheng, 2000);
The detection method of nitrate nitrogen is: salicylic acid colorimetric method for determining (Li Hesheng, 2000);
The detection method of soluble sugar is: anthrone colorimetry measures (Li Hesheng, 2000);
Chlorophyllous detection method is: chlorophyll meter detects;
The detection method of improving activity of root system is: TTC method measures (Li Hesheng, 2000);
Soluble protein detection method: Maas light blue G-250 method measures (Li Hesheng, 2000);
Morphological index assay method: when gathering, examines and records the leaf color of different cultivars, leaf, texture and plant type Feature, and from each kind, select a whole strain as representative, after plant is extracted, clean and dry root system and carry out whole strain The Taking Pictures recording of form;Choose the maximum blade in every strain and carry out the Taking Pictures recording of leaf morphology.When gathering each kind according to Serpentine randomly selects 5 strains (5 repetitions), cuts off from basal part of stem, calculates single-strain blade number by counting method, measures individual plant with ruler Plant height (basal part of stem is to the longest blade tip), leaf length (L, maximum leaf), leaf width (W, maximum leaf), use formula k=A/ (L × W) to calculate Efficient leaf area, wherein correction factor k be 0.7501 (Li Zhenzhen, Zhou Xiaoguang, 2011)
Data analysis: spass22 software
2, result
2.1 water content (see Fig. 1)
Water content difference under different day and night temperatures control is notable, and respectively the water content of process is between 92%-95%, its Middle comparison (CK) water content is 94%, and the water content that W3 processes is the highest, reaches 95%, but and without significant difference between CK.Aqueous Measuring minimum is W1, and its water content is 92%, substantially less than CK.
2.2 soluble proteins (see Fig. 2)
It is more significant that the different temperature difference controls the impact on purple Brassica campestris L soluble protein content, wherein W3 (10.96mg/g) with There is no significant difference between W5 (10.50mg/g), but there were significant differences compared with other each process, W3 and W5 the most relatively CK (8.87mg/ G) improve 23.96%, 18.38%.Showing worst is W6 (5.02mg/g), next to that under W4 (7.50mg/g) the most relatively CK Drop 43.40% and 15.45%.
2.3 Vc (see Fig. 3)
Vitamin C content presents change under the conditions of different differential temperatures, and wherein W1 content is up to 1.91mg/g, with CK (1.49mg/g) there is significant difference, exceed 28.19% than CK, but other respectively organize the most notable with CK change.The vitamin C of W3 Content is minimum in all process, only 1.22mg/g, less than CK about 18.12%.
2.4 chlorophylls (see Fig. 4)
In 6 Temperature Treatment, chlorophyll content size order is W5 > W3 > W1 > W4 > W2 > CK > W6, and W5 content is up to 53.18spad, next to that W3 (48.38spad), is not significantly different between the two, but has significant difference with CK, the most relatively CK Exceed 51.77% and 37.50.W6 (30.64spad) content is minimum, have dropped 12.56% than CK.
2.5 soluble sugars (Fig. 5)
In addition to the soluble sugar content of W1 is higher, other respectively process and between CK difference the most notable.W1 (2.77%) is solvable Property sugar content and CK (1.45%) have obvious difference, improve by about one time than CK, exceed 91.03%.W5 (1.74%), W3 (1.73%), W6 (1.64%) etc. processes soluble sugar content and has exceeded 20% than CK the most respectively, and 19.31%, 13.10%, but It is that difference is the most notable.That soluble sugar content is minimum is W2, only 0.97%, have dropped 33.10% than CK, next to that W4 (1.34%), content relatively CK have dropped 11.00%, but does not all have significant difference.
2.6 anthocyanidin (see Fig. 6)
Anthocyanidin behaves oneself best under the conditions of W3, and content reaches 0.78mg/g, and inferior to W3 is W5 (0.64mg/g) respectively With W2 (0.59mg/g).Three relatively CK has significant difference, W1 anthocyanidin content to be the twices of CK (0.39mg/g), and W5 and The anthocyanidin content of W2 improves 64.10% and 51.28% than CK the most successively.W4 (0.45mg/g) anthocyanidin content the most relatively CK carries High by 15.38%, but difference is the most notable.And the anthocyanidin content the most relatively CK of W1 (0.38mg/g) and W6 (0.28mg/g) is Declining, wherein the content of W1 declines inconspicuous, relatively declines only 2.56%, and W6 have dropped 28.21% than CK, but difference does not shows Write.
2.7 nitrate nitrogens (see Fig. 7)
Nitrate nitrogen is certain positive correlation with the size of day and night temperature, and content size order is W1 < W2 < W3 < W5 < CK <W4<W6.W1 (0.61mg/g), has notable between W2 (0.68mg/g), W3 (1.13mg/g) nitrate nitrogen content and CK (1.71mg/g) Sex differernce, content is respectively lower than CK 64.33%, and 60.23%, 33.92%.W5 (1.6mg/g) nitrate nitrogen content also declines than CK 6.47%, but difference is the most notable.That nitrate nitrogen content is the highest is W6, and content has reached 3.21mg/g, exceeds CK about 87.72%, next to that W4 (2.31mg/g), exceed 35.09% than CK, and and CK between have significant difference.
2.8 improving activity of root systems (see Fig. 8)
Improving activity of root system, intensity is significant difference (Fig. 8) under each temperature treatment conditions, W5 [0.30mg/ (g*h)] improving activity of root system The strongest, in addition to there is no significant difference with W3 [0.22mg/ (g*h)], respectively organize significant difference with other.Wherein, CK The improving activity of root system intensity of [0.09mg/ (g*h)] is minimum.The improving activity of root system intensity of W5, W3 and W1 [0.19mg/ (g*h)] is super Having gone out the twice of CK, wherein W5 is about 3.3 times of CK, and W3 is approximately 2.4 times of CK, and W1 is about 2.1 times of CK.W4[0.16mg/ (g*h)], W2 [0.12mg/ (g*h)], W6 [0.11mg/ (g*h)] improve 77.78% than CK the most successively, and 33.33%, 22.22%, but between three's improving activity of root system intensity and CK, difference is the most notable.
The sequence of above-mentioned physical signs is shown in Table 2:
Table 2: physical signs list
Note: is the value put up the best performance with circle mark.
2.9 forms, yield index (be shown in Table 3, table 4, Fig. 9, Figure 10)
Table 3: morphological index
Note: is the value put up the best performance with circle mark.
Table 4: yield index
Note: is the value put up the best performance with circle mark.
2.10 principal component analysiss (be shown in Table 5 and table 6)
Table 5: index of quality main constituent part analysis result
Table 6: aggregative indicator principal component analysis result
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned Particular implementation, those skilled in the art can make various deformation or amendment within the scope of the claims, this not shadow Ring the flesh and blood of the present invention.

Claims (10)

1. the purple rapeseed cultivation method controlled based on the temperature difference, it is characterised in that described method is specially and is placed in by purple Brassica campestris L Daytime, temperature grew under conditions of there is the temperature difference with nocturnal temperature.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 1, it is characterised in that described daytime temperature In the range of 20-30 DEG C, nocturnal temperature is in the range of 10-20 DEG C.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 1 and 2, it is characterised in that described daytime Temperature, being combined as of nocturnal temperature: daytime temperature 30 DEG C, nocturnal temperature 20 DEG C;Daytime temperature 25 DEG C, nocturnal temperature 15 DEG C;In vain Daytime temperature 20 DEG C, nocturnal temperature 10 DEG C;Daytime temperature 30 DEG C, nocturnal temperature 15 DEG C;Daytime temperature 25 DEG C, nocturnal temperature 10 DEG C; Daytime temperature 30 DEG C, nocturnal temperature 10 DEG C.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 3, it is characterised in that described daytime temperature Degree, being combined as of nocturnal temperature: daytime temperature 30 DEG C, nocturnal temperature 20 DEG C;Daytime temperature 20 DEG C, nocturnal temperature 10 DEG C;Daytime Temperature 25 DEG C, nocturnal temperature 10 DEG C.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 4, it is characterised in that described daytime temperature Degree, being combined as of nocturnal temperature: daytime temperature 30 DEG C, nocturnal temperature 20 DEG C;Daytime temperature 20 DEG C, nocturnal temperature 10 DEG C.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 5, it is characterised in that described daytime temperature Degree, being combined as of nocturnal temperature: daytime temperature 20 DEG C, nocturnal temperature 10 DEG C.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 1 and 2, it is characterised in that described purple oil Dish SANYE wholeheartedly when transplanted in daytime temperature exist with nocturnal temperature and to grow under conditions of the temperature difference.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 1 and 2, it is characterised in that described growth Ambient humidity be 85%.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 1 and 2, it is characterised in that described growth During, fertilizer uses green slow-release fertilizer difficult to understand, every liter of substrate 4g.
The purple rapeseed cultivation method controlled based on the temperature difference the most according to claim 1 and 2, it is characterised in that described growth Growth cabinet is carried out.
CN201610427137.4A 2016-06-15 2016-06-15 Method for planting brassica campestris based on temperature difference control Expired - Fee Related CN106069013B (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1125504A (en) * 1994-12-28 1996-07-03 华中农业大学 Hybrid seeding method of high-yield, high-quality and wild cabbage type rape
CN102301898A (en) * 2011-07-29 2012-01-04 江苏丘陵地区镇江农业科学研究所 High-yield cultivation method of double-low rape flower
CN102986335A (en) * 2012-12-15 2013-03-27 安徽科技学院 Method for raising seedling in rape chamber by utilizing LED (light-emitting diode) light source
CN104838874A (en) * 2015-05-29 2015-08-19 扬州大学 Rape planting method capable of prolonging rape flower viewing period
CN105601386A (en) * 2016-01-15 2016-05-25 华中农业大学 Hydroponic culture solution and oversummer culture method for cabbage type rape microspore seedlings

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1125504A (en) * 1994-12-28 1996-07-03 华中农业大学 Hybrid seeding method of high-yield, high-quality and wild cabbage type rape
CN102301898A (en) * 2011-07-29 2012-01-04 江苏丘陵地区镇江农业科学研究所 High-yield cultivation method of double-low rape flower
CN102986335A (en) * 2012-12-15 2013-03-27 安徽科技学院 Method for raising seedling in rape chamber by utilizing LED (light-emitting diode) light source
CN104838874A (en) * 2015-05-29 2015-08-19 扬州大学 Rape planting method capable of prolonging rape flower viewing period
CN105601386A (en) * 2016-01-15 2016-05-25 华中农业大学 Hydroponic culture solution and oversummer culture method for cabbage type rape microspore seedlings

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘后利: "《油菜的遗传和育种》", 31 August 1985, 上海科学技术出版社 *
王敏; 曲存民;刘晓兰;谢景梅;李加纳: "温度胁迫下甘蓝型油菜苗期生理生化指标的研究", 《作物杂志》 *

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