CN109197578B - Breeding method of P-group maize inbred line Lx286 - Google Patents
Breeding method of P-group maize inbred line Lx286 Download PDFInfo
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- CN109197578B CN109197578B CN201811395554.0A CN201811395554A CN109197578B CN 109197578 B CN109197578 B CN 109197578B CN 201811395554 A CN201811395554 A CN 201811395554A CN 109197578 B CN109197578 B CN 109197578B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention relates to a breeding method of a P-group maize inbred line Lx286, which is characterized in that Y928 and C486 are used as parents to construct S of a basic material Y928/C486 in the south of Jinan0Generation; in Hainan pair S0Selfing to obtain S1While using the test seeds C138 and S0Substitute combination to obtain the combination C138/S0(ii) a According to the hybridization combination C138/S0In the yield and the field performance (lodging resistance and disease resistance) of the Jinan, the descendant of the basic population established by Y928/C486 is considered to be suitable for line selection; planting Y928/C486 in Gansu Zhangye1Selecting early-maturing S with excellent comprehensive character from the progeny group2Carrying out single plant cultivation; in Hainan will S2Planting single plant into ear rows, testing and matching the ear rows with test seed C138, and selecting S with excellent comprehensive character during harvesting3Ear row; s of the selected ear row in Jinan3Individual plant species are grown into ears and selfed to obtain S4Combining field performance and production results according to the test and match, and S3Selecting S with higher combining ability through comprehensive performance of panicle rows4(ii) a Bagging and selfing are continuously carried out in Hainan and Jinan, and finally, a maize selfing line Lx286 is bred according to test results of different test seeds.
Description
Technical Field
The invention belongs to the technical field of agricultural science, and particularly relates to a breeding method of a P-group maize inbred line Lx 286.
Background
The breeding is a long-period work, the breeding time of a variety is generally 10-15 years, most of the breeding time is used for line selection, the breeding process is actually a line selection process, and the line selection is an important link of corn breeding. The line selection is to purposefully realize the continuous recombination and polymerization of excellent genes and create a new inbred line. Has excellent inbred line and can certainly prepare excellent hybrid seeds.
In order to ensure the success rate of breeding the inbred line, methods such as large population, high-density screening, early generation combining ability testing and the like are generally adopted, and simultaneously with the development of a corn breeding technology, the method for breeding the corn inbred line by utilizing the induction line to induce the haploid through hybridization becomes possible, so that the breeding period can be shortened to 2 years. However, we only focus on the improvement of the technical means, and do not realize from the beginning whether the matched corn base material is suitable for line selection, namely whether the offspring of the group has high combining ability, and if the offspring group does not have good combining ability, all the technical means do not work. Meanwhile, whether the bred inbred line has high combining ability but can meet the requirements of seed production in northwest is not concerned, and particularly, the P group inbred line containing tropical blood margin is sensitive to light and temperature, has a late flowering phase, has long flowering phase interval time between parents and has high risk on seed production.
Disclosure of Invention
In order to solve the problems, the invention provides a breeding method of a P-group maize inbred line Lx 286. The invention provides a method for rapidly identifying the high and low matching force of a corn basic material, and simultaneously provides a method for breeding a P-group-containing corn inbred line suitable for northwest seed production climate. The maize inbred line Lx286 bred by the breeding method provided by the invention has high combining ability, strong disease resistance and moderate mature period, and can completely meet the requirements of seed production in northwest.
The technical scheme of the invention is as follows: a breeding method of a P-group maize inbred line Lx286 comprises the following steps:
1) hybridizing Y928 as female parent and C486 as male parent in Jinan to obtain S of Y928/C4860As a base material; y928 is improved strain of Ye 478, and C486 is inbred strain bred from foreign hybrid 78599.
2) Planting in Hainan0Inbred to obtain S1Simultaneously hybridizing the test strain C138 as a male parent to obtain a combined C138/S0；
3) Planting combination C138/S in Jinan0And control Zhengdan 958, the combination was combined after harvest at maturityPerforming seed test and yield measurement on fruit clusters;
4) planting Y928/C486 in Gansu Zhangye1Selecting early-maturing individual plant from the progeny group, selfing to obtain S2And selecting S with excellent comprehensive character during maturation2Carrying out single plant cultivation;
5) in Hainan will S2Planting single plant in ear row, selecting excellent ear row for selfing to obtain S3And the lines were tested for their capacity using C138;
6) planting and testing combination in the south of Ji, investigating field characters and testing yield, and simultaneously continuously selecting S of the ear rows in the south of Ji3The single plant is grown into ears, and selfed to obtain S4Selecting the panicle rows with higher combining ability according to the field performance and the testing and matching result of the panicle rows;
7) s of selected ear row in Hainan4Planting single plant, ear-row, bagging and selfing to obtain S5(ii) a And testing and matching the ears by using inbred lines of test seeds Lx721, D382, J724 and the like;
8) the test cross breeds are respectively subjected to primary identification in the places of Jinan, Heze, Linyi and the like, the result shows that the ear walking combining ability of the serial number 286 is higher, the combined hybrid breed shows high yield, lodging resistance and disease resistance, and the yield is increased by more than 4.5 percent compared with the contrast Zhengdan 958; finally, the excellent ear inbred line is named as an inbred line Lx 286.
Preferably, step 2), the test strain C138 is a self-bred line which is obtained by multi-generation self-breeding of foreign hybrid species Yu 335.
Preferably, step 3) shows that the basic material Y928/C486 has higher combining ability by measurement, and the selection can be selected again.
Preferably, step 5), these ears are subjected to a mating force test using test species C138.
The invention has the beneficial effects that:
1) the breeding method of the maize inbred line LX286 provided by the invention is that Y928 and C486 are used as parent and parent building basic materials S0With S0As female parent, test species C138 is female parent combination C138/S0According to the hybridization combination C138/S0The yield and the comprehensive performance in the field,can quickly judge the basic group S0And (4) judging whether the selection is suitable for further selection according to the combining force.
2) Will S1The generation large population single plant is directly planted in Gansu Zhangye to breed early-maturing single plant containing P group and suitable for local climate condition, and further through the test and matching of south breeding and north breeding and test seed to self-bred progeny, a self-bred line with moderate maturing period, high matching force, strong resistance and good fruit cluster fruition is selected.
3) The maize inbred line Lx286 bred by the breeding method provided by the invention has high combining ability, strong disease resistance and moderate mature period, and can completely meet the requirements of seed production in northwest.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As described in the background, breeding is a long-period work, the time for breeding a variety is generally 10-15 years, most of the breeding time is used for line selection, the breeding process is actually a line selection process, and the line selection is an important link of corn breeding. The line selection is to purposefully realize the continuous recombination and polymerization of excellent genes and create a new inbred line. Has excellent inbred line and can certainly prepare excellent hybrid seeds.
The invention provides a breeding method of a P-group maize inbred line Lx286, which comprises the following steps:
1) using Y928 as mother root and C486 as mother rootMale parent, hybridized in Jinan to obtain S of Y928/C4860As a base material;
2) planting in Hainan0Inbred to obtain S1Simultaneously hybridizing the test strain C138 as a male parent to obtain a combined C138/S0；
3) Planting combination C138/S in Jinan0And control Zhengdan 958, the combination and control were seeded after harvest at maturity and tested for yield. The test of species and the measurement of production show that the basic material Y928/C486 has higher combining ability and can be selected again;
4) planting Y928/C486 in Gansu Zhangye1Selecting early-maturing individual plant from the progeny group, selfing to obtain S2And selecting S with excellent comprehensive character during maturation2Carrying out single plant cultivation;
5) in Hainan will S2Planting single plant in ear row, selecting excellent ear row for selfing to obtain S3And the test seeds C138 are used for carrying out the combining ability test on the panicles;
6) planting and testing combination in the south of Ji, investigating field characters and testing yield, and simultaneously continuously selecting S of the ear rows in the south of Ji3The single plant is grown into ears, and selfed to obtain S4Selecting the panicle rows with higher combining ability according to the field performance and the testing and matching result of the panicle rows;
7) s of selected ear row in Hainan4Planting single ear, bagging and selfing to obtain S5. And testing and matching the ears by using inbred lines of test seeds Lx721, D382, J724 and the like;
8) the test cross breeds are respectively subjected to primary identification in the places of Jinan, Heze, Linyi and the like, the result shows that the ear walking combining ability of the serial number 286 is higher, the combined hybrid breed shows high yield, lodging resistance and disease resistance, and the yield is increased by more than 4.5 percent compared with the contrast Zhengdan 958; finally, the excellent ear inbred line is named as an inbred line Lx 286.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
In the 2006, in Jinan in summer, Y928 as female parent and C486 as male parent were hybridized to obtain S of Y928/C4860And the substitute is used as a basic material. Wherein Y928 is an improved strain of Ye 478, and C486 is an inbred strain bred from foreign hybrid 78599.
Planting S in Hainan in winter in 20060S obtained by selfing in bags1Meanwhile, the test species C138 is used as female parent to obtain the combination C138/S0。
The combination C138/S is planted in Jinan in summer in 20070And Zhengdan 958, 5 m row length, 10 row area, density 4500 plants/mu, field investigation of agronomic characters, finding combination C138/S0Resisting diseases and lodging, harvesting 6 rows in the middle of the field after maturity, testing seeds, threshing, measuring yield and weighing. The yield test result shows that the combined yield is increased by more than 5.0 percent compared with the control Zhengdan 958, which indicates that the basic population Y928/C486 obtained by hybridization has higher combining ability and better field agronomic performance, and can select lines by re-selection.
Meanwhile, in 2007, Y928/C486 is planted in Gansu Zhangye in summer in high density1Selecting individual single plants with early pollen scattering period, sufficient pollen amount and coordinated female and male ear flowering periods for bagging and selfing for generation progeny groups (3000-plus 5000 plants), selecting single plants with disease resistance, lodging resistance and good fruit cluster bearing property during harvesting, selecting excellent single ears through seed test and threshing to obtain S2And (4) seeds.
2008, winter in Hainan will be S2And planting single spike rows into 200 spike rows in total, selecting 2 rows of regions of each spike row, selfing the spike rows with normal and consistent growth vigor, moderate plant height and spike position ratio, coordinated flowering phase and good resistance, bagging and selfing the spike rows, selfing 10 spikes in each row, and testing the combining ability of the selfed spike rows by using a test seed C138. Screening 30 ear rows according to disease resistance and fruit cluster bearing capacity during harvesting, and performing seed test and single ear threshing to obtain S3And (4) seeds.
In 2009, test crossing combinations of selected ear rows are planted in the south of the Jinan, each cell is 5 m in length, 4 rows in area and density of 4500 plants/mu, agronomic characters of the plants are investigated in the field, and after the plants are mature, 2 rows of middle plants are harvested, threshed, tested, and weighed. In summer of the same year, S of selecting the ear rows in the Jinan3The single ear is planted into ear rows, 5 ears are taken from each ear row, and the total number of the ear rows is 150. Determining the high combining ability according to the disease resistance, the fruit cluster bearing ability and the combined combining ability determination result during harvesting10 ear rows, and obtaining S through seed examination and threshing4And (4) seeds.
In 2009, S is collected in Hainan in winter4Planting spike rows with single spike, taking 10 spikes from each spike row, totally 100 spike rows, eliminating the spike rows with weak growth potential, poor disease resistance and less pollen amount, bagging the rest spike rows, and selfing to obtain S5. And testing the ears by using inbred lines of test strains Lx721, D382, J724 and the like.
The first identification of the test hybrids in Jinan, Neze, Linyi and the like in summer in 2010 shows that the combining ability of the panicle number 286 is higher, the combined hybrid shows high yield, lodging resistance and disease resistance, and the yield is increased by more than 4.5 percent compared with the control Zhengdan 958. And finally, the excellent ear row selfing line is named as a selfing line Lx286, meanwhile, the single ear row bagging selfing of the selfing line is continuously screened, more test seeds are used for testing and matching, and an excellent hybrid combination is selected.
The Lx286 has high matching force, strong disease resistance and moderate mature period, and can completely meet the requirements of seed production in northwest China. Practice proves that the method is reasonable and effective, can quickly judge the combining ability of the basic materials, can breed the inbred line suitable for northwest climate, and meets the requirements of seed production.
Application of inbred line Lx286
Ludan 215: the Ludan 215 is a medium-maturing corn hybrid formed by combining S485 as a female parent and Lx286 as a male parent, and the average yield per mu of 638.6 kg in a comparison test of the yield of a corn research institute of Shandong academy of agricultural sciences in 2015 is increased by 8.5 percent compared with a control Zhengdan 958.
In a density group pre-test of 4500 plants/mu in Shandong province in 2016, the average yield per mu is 698.4 kg, and the yield is increased by 8.4 percent, 8 percent by 1 percent by comparison with a control Zhengdan 958, and the yield is increased by more than 88.9 percent.
In the first year area test of the common group (4500 plants/mu) of summer maize varieties in sanjiashandong province in 2017, the average yield per mu is 726.7 kilograms, the yield is increased by 7.8 percent compared with that of a control Zhengdan 958, the yield is increased at 13 test points, 11 points and 2 points.
Of course, the above description is not limited to the above examples, and the undescribed technical features of the present invention can be implemented by or using the prior art, and will not be described herein again; the above embodiments are merely for illustrating the technical solutions of the present invention and not for limiting the present invention, and the present invention has been described in detail with reference to the preferred embodiments, and those skilled in the art should understand that changes, modifications, additions or substitutions which are made by those skilled in the art within the spirit of the present invention are also within the scope of the claims of the present invention.
Claims (3)
1. A breeding method of a P-group maize inbred line Lx286 is characterized by comprising the following steps: the method comprises the following steps:
1) hybridizing Y928 as female parent and C486 as male parent in Jinan to obtain S of Y928/C4860As a basic material, Y928 is an improved line of fluid 478, and C486 is an inbred line bred from foreign hybrid 78599;
2) planting in Hainan0Inbred to obtain S1Meanwhile, the test species C138 is used as female parent to obtain the combination C138/S0C138 is a self-bred line which is obtained by carrying out multi-generation self-breeding on foreign hybrid jade 335;
3) planting combination C138/S in Jinan0And control Zhengdan 958, and after the harvest after maturation, performing seed test on the combination and control ears and measuring yield;
4) planting Y928/C486 in Gansu Zhangye1Selecting early-maturing individual plant from the progeny group, selfing to obtain S2And selecting S with excellent comprehensive character during maturation2Carrying out single plant cultivation;
5) in Hainan will S2Planting single plant in ear row, selecting excellent ear row for selfing to obtain S3And the lines were tested for their capacity using C138;
6) planting and testing combination in the south of Ji, investigating field characters and testing yield, and simultaneously selecting S of selected panicles in the south of Ji3Planting single plant into ear row and selfing to obtain S4Selecting the panicle rows with higher combining ability according to the field performance and the testing and matching result of the panicle rows;
7) s of selected ear row in Hainan4Planting single plant, ear-row, bagging and selfing to obtain S5(ii) a And using the test seed inbred line pairThese rows were run for testing.
2. The breeding method of the P-group maize inbred line Lx286 according to claim 1, wherein the yield measurement in step 3) shows that the basic material Y928/C486 has high combining ability and can be selected by re-selection.
3. The method of claim 1, wherein in step 5), the lines are tested for fitness using test C138.
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