CN115088611B - Breeding method of high-resistance starch hybrid rice - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
- A01H1/101—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine
- A01H1/102—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
Abstract
The invention discloses a breeding method of high-resistance starch hybrid rice, which comprises the following steps ofComprising the following steps: hybridization and m times of backcross are carried out on the Zhejiang high-straight 1B serving as a male parent and a backcross parent and a sterile line AII-32A of a new hybrid rice variety II-7954; and backcrossing with the male parent Zhejiang high-straight 1B for multiple times to obtain sterile line A' 6 The method comprises the steps of carrying out a first treatment on the surface of the Then, the restorer R Zhehui 7954 of the new hybrid rice variety II you 7954 is used as a male parent and a backcross parent, and is hybridized with Yitangjing No. 1 and backcrossed for n times; then the hybrid combination with high yield and high quality disease resistance and the corresponding restoring line are obtained through continuous test crossing with the sterile line; finally, a sterile line A 'is constructed' 6 Restorer line R' 6 And a seed breeding production system for preparing a new variety of the high-resistance starch hybrid rice, and breeding the high-resistance starch hybrid rice. The method cultivates the high-resistance starch hybrid rice, has the advantages of high-resistance starch function and health and excellent special processing performance of rice noodles, and is suitable for light simplified cultivation and high yield and efficiency.
Description
Technical Field
The invention relates to the technical field of rice breeding, in particular to a breeding method of high-resistance starch hybrid rice.
Background
According to the health China action estimation, the prevalence rate of diabetes in China is 12.8 percent, and the patient is 1.5 hundred million; the prevalence of dyslipidemia is 18.6% and the patient is 1.6 billion. Resistant Starch (RS) is a general term for starch and starch degradation products which are difficult to digest and absorb in the small intestine of a healthy human body, and has a plurality of important physiological functions of reducing blood sugar and blood fat, controlling weight, being beneficial to intestinal health and the like.
The research of high-resistance starch rice is carried out by research units such as Zhejiang university, chinese academy of sciences genetic development institute, zhejiang green giant biotechnology Limited company and the like in China, and the novel strain of high-resistance starch conventional rice such as Zhejiang 111, zhejiang Jiuzhang No. 1 and Zhejiang Jiuzhen No. 1 and the high-resistance starch indica hybrid rice such as Zhejiang Jiujiang No. 1 are bred successively, but the obvious problems of lower taste and yield exist. The high-resistance starch rice can better develop functions of nutrition and high yield and high efficiency if being combined with high-yield high-quality and special hybrid rice.
Therefore, the breeding method of the high-resistance starch hybrid rice provided by the invention has the function of high-resistance starch and excellent special processing performance of rice noodles, and is suitable for light and simplified cultivation.
Disclosure of Invention
The invention provides a breeding method of high-resistance starch hybrid rice, which is used for cultivating and retaining the basic characteristics of target hybrid varieties, has the function of high-resistance starch, excellent special processing performance of rice noodles, and is high in yield and lodging-resistant.
The specific technical scheme is as follows:
a breeding method of high-resistance starch hybrid rice comprises the following steps:
(1-1) hybridization and m times of backcrossing with sterile line AII-32A of new hybrid rice variety IIU 7954 with ZheGao straight 1B as male parent and backcross parent to obtain BC m F 1 Seed generation;
(1-2) planting BC m F 1 The sterile plant is selected to be backcrossed with Zhejiang high-straight 1B to obtain BC m+1 F 1 Seed generation;
(1-3) planting BC m+1 F 1 Instead, determining and selecting sterile line A 'with high sterile grain resistant starch' 1 The method comprises the steps of carrying out a first treatment on the surface of the Backcross with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 2 Seed;
(1-4) planting sterile line A' 2 Sterile line II-32A is used as a reference to identify sterile line A' 2 Sterile line A 'with thorough sterility is finally selected and remained' 3 Backcross with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 4 Seed;
(1-5) planting sterile line A' 4 Selecting and reserving sterile line A 'conforming to the characteristics of the sterile line' 5 Backcross with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 6 Seed;
2) And (3) breeding a restoring line:
(2-1) the restorer line R Zhehui 7954 of the new hybrid rice variety II you 7954 is taken as the male parentAnd backcrossing parent, hybridization with sugar-free japonica No. 1 and n times backcrossing to obtain BC n F’ 1 Seed generation;
(2-2) planting BC n F’ 1 Instead, after selfing, BC is harvested n F’ 2 Seed generation;
(2-3) planting BC n F’ 2 Instead, select and leave the restorer R 'with high resistant starch' 1 With sterile line A' 1 Paired test cross to obtain restorer R' 2 Seed;
(2-4) planting restorer line R' 2 The recovery line R Zhehui 7954 and the new hybrid rice variety II you 7954 are used as the contrast to observe the hybrid combination A' 2 ×R’ 2 Excellent hybridization combination with high selection and retention resistant starch and corresponding restorer line R' 3 With sterile line A' 3 Paired test cross to obtain restorer R' 4 Seed;
(2-5) planting restorer line R' 4 Identification of superior hybridization combinations A' 4 ×R’ 4 The yield and physical and chemical quality of (2) are selected and remained high-yield high-quality hybrid combination and corresponding restorer line R' 5 Obtaining a restorer line R' 6 Is a seed of (2);
3) Construction of sterile line A' 6 Restorer line R' 6 And preparing a seed breeding production system of the new variety of the high-resistance starch hybrid rice, and finally breeding the high-resistance starch hybrid rice.
Wherein, the Zhejiang high-straight 1B is a corresponding maintainer line of the high-resistance starch and amylose content hybrid rice sterile line Zhejiang high-straight 1A bred at Zhejiang university, which has been approved by the crop variety approval Committee of Zhejiang province, and the approval certificate number is Zhejiang rice (sterile line) 2022006. Meanwhile, the sterile line has applied for and authorized new plant species rights (species rights number: 20211005743) to the agricultural rural area.
Sugar-suitable japonica 1 is a new high-resistance starch japonica rice variety cooperatively bred by Zhejiang green giant biotechnology limited company and Zhejiang university, and has been applied to the agricultural rural area and authorized for the right of the new plant variety (application number: CNA 20140191.9).
The target hybrid rice new variety is a three-line indica hybrid rice new variety matched with a restoring line Zhehui 7954 by utilizing a sterile line II-32A of Zhejiang province agricultural academy of sciences, namely the parent sterile line A is II-32A, and the restoring line R is Zhehui 7954. II, U.S. Pat. No. 7954 was approved by the crop variety approval Committee of Zhejiang province in 2002, and the national crop variety approval Committee was recommended in 2003, and was recognized by the Ministry of agriculture as a super rice variety for major popularization nationally in 2005.
Further, in the step (1-1), m is less than or equal to 3; in the step (2-1), n is less than or equal to 3.
Further, the judgment standard of high resistant starch content is that the resistant starch content is more than or equal to 10.0%.
Further, in the step (1-4), the judging standard of sterility is that the pollen abortion rate is more than or equal to 99.99% and the bagging fruiting rate is less than or equal to 0.01%.
Further, in the step (2-5), the judgment standards of high yield and high quality are as follows: the yield is more than or equal to 550 kg/mu, and the amylose content of the physicochemical index is more than or equal to 30%.
Compared with the prior art, the invention has the following beneficial effects:
(1) The invention breeds a new variety of high-resistance starch indica-japonica type partial indica hybrid rice based on the resistant starch and the target indica hybrid rice variety, and has the advantages of excellent special processing performance of rice lines while maintaining the function of the resistant starch.
(2) The invention adopts the resistant starch with equal resistance but genetic background of indica type and japonica type as parent, and separately improves sterile line and restorer line, which can avoid common problems of rice breeding homogeneity, lay genetic foundation for high-yield and high-quality hybrid vigor of resistant starch hybrid rice, and remarkably embody strong stalk, good lodging resistance and obvious yield advantage.
Detailed Description
The invention will be further described with reference to the following examples, which are given by way of illustration only, but the scope of the invention is not limited thereto.
EXAMPLE 1 fertility genetic analysis of Zhejiang high-straight 1B and II-32A filial generations
The paired hybridization identification of the high-resistance starch new variety Zhejiang high-straight 1B and II-32A shows that: the resistant starch content of Zhejiang Gaohuang 1B is high, pollen is fertile, and the bagging is firm; II-32A has low resistant starch content, completely sterile pollen, bagging set percentage of 0.01 and representative water-printing sterile line; II-32A is as high as 1B, the sterility is as same as that of II-32A, the pollen is completely sterile, the bagging maturing rate is 0, but the resistant starch is obviously improved (table 1), which provides precondition for directly breeding resistant starch sterile line through hybridization and backcross.
TABLE 1 genetic expression analysis of resistant starch and II-32A in Zhejiang Gaohuang 1B
| Hybrid F 1 Characteristic properties | Resistant starch content | Group sports performance | Pollen fertility | Set bag setting rate |
| Zhejiang Gaohang 1B | 10.9％ | Fully fertile | Fertility, 99.99% | 96.4％ |
| Ⅱ-32A | 0.9％ | Sterility (infertility) | Sterility of 99.99% | 0.01％ |
| II-32A x Zhejiang high straight 1B | 3.8％ | Sterility (infertility) | Sterility of 99.99% | 0.0％ |
EXAMPLE 2 genetic analysis of resistant starch in Zhejiang Gaohuang 1B and Yitangjing 1
The combination identification of Zhejiang Gaohuang 1B and Yitang japonica No. 1 positive and negative cross shows that: the resistant starch of the positive and negative cross combination is high, which shows that the resistant starch in Zhejiang Gaohuang No. 1B and Yitang japonica No. 1 are equal (table 2), which provides a technical basis for targeted independent improvement of sterile line and restorer line; in addition, the genetic background of ZheDagaohuan 1B or Yitang japonica 1 indica-japonica is selected as the donor of the resistant starch, so that the homogenization can be avoided, and a foundation is laid for the high yield and high quality of the resistant starch hybrid rice.
TABLE 2 genetic analysis of resistant starch in Zhejiang Gaohuang 1B and Yitangjing 1
Example 3 selection method of high resistance starch hybrid Rice based on Zhejiang Gaohuang 1B, yitang japonica No. 1 and II you 7954 (sterile line II-32A, restorer line Zhejiang 7954)
The Zhejiang high-straight 1B is a corresponding maintainer line of a high amylose and resistant starch hybrid rice sterile line bred at Zhejiang university, and has passed the approval of the crop variety approval committee of Zhejiang province, and the approval certificate number is Zhejiang rice (sterile line) 2022006. Meanwhile, the sterile line has applied for and authorized new plant species rights (species rights number: 20211005743) to the agricultural rural area. The strain of the strain has a height of 91cm, resistant starch content of 10.9%, amylose content of 38.6% and yield of 400 kg/mu, and can be purchased from the university of Zhejiang nuclear agricultural science institute.
Sugar-suitable japonica 1 is a new high-resistance starch japonica rice variety cooperatively bred by Zhejiang green giant biotechnology limited company and Zhejiang university, and the new plant variety weight (variety weight number: CNA 20140191.9) has been applied and authorized to the agricultural rural area. The strain has a height of 85cm, resistant starch content of 10.7%, amylose content of 28.4% and yield of 400 kg/mu, and can be purchased from Zhejiang green giant biotechnology Co.
Iyou7954 is a new three-line indica hybrid rice variety matched with a recovery line Zhehui 7954 by using sterile line II-32A of Zhejiang province agricultural academy of sciences. The combination passes the approval of the agricultural crop variety approval committee in Zhejiang province in 4 months 2002, recommends the approval of the national agricultural crop variety approval committee in 2003, and is recognized by the agricultural department as a super rice variety which is promoted nationally in a major way in 2005. The strain height of the variety is 118.9cm, the resistant starch is 1.5%, the amylose content is 25.2%, and the yield is 550 kg/mu, and the variety can be purchased from the national academy of agricultural science in Zhejiang province.
A breeding method of high-resistance starch hybrid rice comprises the following specific steps:
breeding a high-resistance starch sterile line:
(1) Hybridization and 2 backcrosses are carried out between the Zhejiang high-straight 1B serving as a male parent and a backcross parent and a sterile line AII-32A of a new hybrid rice variety II-7954 of a target hybrid rice variety II 2 F 1 Seed generation; the restorer R Zhehui 7954 of the new hybrid rice variety II you 7954 is used as the male parent and the backcross parent, and is hybridized with Yitang japonica No. 1 and backcross for 2 times to obtain BC 2 F’ 1 Seed generation;
(1-2) planting BC 2 F 1 The generation, selecting sterile 16 strains, backcrossing with Zhejiang high-straight 1B to obtain BC 3 F 1 Seed generation;
(1-3) planting BC 3 F 1 The generation, the sterile plant is measured, and then the sterile line A 'with the grain resistant starch more than or equal to 10.0% is selected' 1 8. A plurality of; backcross with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 2 8 seeds;
(1-4) planting sterile line A' 2 Sterile line II-32A is used as a reference to identify sterile line A' 2 The sterile rate of the finally selected and remained pollen is more than or equal to 99.Sterile line A 'with 99% and bagging setting rate less than or equal to 0.01%' 3 5, backcrossing with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 4 5 seeds;
(1-5) planting sterile line A' 4 Selecting and reserving sterile line A 'with sterility conforming to the characteristics of sterile line' 5 2, backcrossing with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 6 1 seed.
2) And (3) breeding a restoring line:
(2-1) hybridization and 2 backcrossing with sugar-free japonica No. 1 by using a restorer line R Zhehui 7954 of a new hybrid rice variety II you 7954 as a male parent and a backcross parent to obtain BC2 n F’ 1 Seed generation;
(2-2) planting BC 2 F’ 1 Instead, after selfing, BC is harvested 2 F’ 2 Seed generation;
(2-3) planting BC 2 F’ 2 Instead, a restorer R 'with grain resistant starch not less than 10.0% is selected' 1 21, with sterile line A' 1 Paired test cross to obtain restorer R' 2 16 seeds;
(2-4) planting restorer line R' 2 The recovery line R Zhehui 7954 and the new hybrid rice variety II you 7954 are used as the contrast to observe the hybrid combination A' 2 ×R’ 2 6 excellent hybridization combinations with resistant starch more than or equal to 10.0% and corresponding restorer line R 'are selected' 3 6, with sterile line A' 3 Paired test cross to obtain restorer R' 4 Seed;
(2-5) planting restorer line R' 4 Identification of superior hybridization combinations A' 4 ×R’ 4 The yield, physicochemical quality and disease resistance of the strain are selected and reserved for hybridization, wherein the yield is more than or equal to 550 kg/mu, the amylose content of physicochemical indexes is more than or equal to 30%, 2 hybridization combinations and corresponding restorer lines R' 5 2, a restorer line R 'was obtained' 6 Is a seed of (2);
3) Construction of sterile line II-32A' + resistant starch (i.e. A' 6 ) Zhehui 7954' + resistant starch (i.e. R' 6 ) And preparing a seed breeding production system of the new variety of the resistant starch hybrid rice, and finally breeding 1 high-resistance starch hybrid rice,temporary name "anti-powder hybridization No. 1".
The index characters of the indica-japonica type, the growth period, the resistant starch content, the plant height, the main stem thickness, the rice yield, the rice quality, the amylose content and the rice noodle processing performance of each variety are compared in table 3.
Table 3 comparison of index characters of Zhejiang Gaohuang 1B, yitang japonica No. 1, II you 7954 and anti-powder hybridization No. 1
Remarks: * The combination of the anti-powder hybridization No. 1 and each parent and the target hybridization is compared with the 0.05 level extremely significant difference
The measurement methods of the respective indexes in table 3 are as follows: plant height, stalk thickness, lodging resistance and rice yield: national rice variety test observation record items, methods and standards (trial); resistant starch: AOAC 991.43; rice quality and amylose content: NY/T593-2013.
Through joint evaluation and identification, the bred high-resistance starch hybrid rice is 'anti-powder hybrid No. 1', resistant starch is 10.8%, plant height is 103cm, rice yield is 570 kg/mu, and amylose content is 32.5%.
Compared with two resistant starch parents and target hybrid rice varieties, the 'resistant starch hybrid No. 1' has the advantages of both parents, but the improvement of the restorer line increases the components of the resistant starch donor 'Yitang japonica No. 1' japonica rice, which is represented by the components with japonica in indica type, the increase of genetic distance is obviously reflected in thicker main stems (1.15 cm), the rice yield is obviously improved, the plant height is obviously reduced, the lodging resistance is obviously enhanced, and the method is superior to the expected breeding target. In addition, the amylose content of the anti-powder hybridization No. 1 is very high, which is obvious that the special processing performance of rice noodles is remarkably better: the content of the high-purity high-1B amylose is too high, and the rice noodles are easy to break; sugar japonica 1 has higher amylose content, but rice noodles prepared from japonica rice are sticky and easy to adhere; the target hybrid rice variety II is good in quality, 7954 has medium amylose content, is slow to retrograde during production, and has general rice noodle performance; the anti-powder hybridization No. 1 has high amylose content, and the rice noodle is high in quality and continuous, so that the special processing performance exceeds the breeding expectation, and the anti-powder hybridization No. 1 is very beneficial to quality improvement, synergy and healthy and delicious life.
Therefore, the 'anti-powder hybridization No. 1' bred by the method has the advantages of excellent special processing performance of rice noodles besides the healthy function of high-resistance starch, resists lodging, is suitable for light simplified cultivation, and saves labor and cost.
Claims (4)
1. A breeding method of high-resistance starch hybrid rice is characterized by comprising the following steps:
1) Breeding sterile line:
(1-1) hybridization and m backcrossing with the sterile line II-32A of the new hybrid rice variety II-well 7954 with ZheGao straight 1B as male parent and backcross parent to obtain BC m F 1 Seed generation; m is less than or equal to 3;
(1-2) planting BC m F 1 The sterile plant is selected to be backcrossed with Zhejiang high-straight 1B to obtain BC m+1 F 1 Seed generation;
(1-3) planting BC m+1 F 1 Instead, determining and selecting sterile line A 'with high sterile grain resistant starch' 1 The method comprises the steps of carrying out a first treatment on the surface of the Backcross with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 2 Seed;
(1-4) planting sterile line A' 2 Sterile line II-32A is used as a reference to identify sterile line A' 2 Sterile line A 'with thorough sterility is finally selected and remained' 3 Backcross with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 4 Seed;
(1-5) planting sterile line A' 4 Selecting and reserving sterile line A 'conforming to the characteristics of the sterile line' 5 Backcross with the male parent Zhejiang high-straight 1B to obtain a sterile line A' 6 Seed;
2) And (3) breeding a restoring line:
(2-1) hybridization and n times of backcrossing with sugar-free rice No. 1 by taking a restorer line Zhehui 7954 of a new hybrid rice variety II you 7954 as a male parent and a backcross parent to obtain BC n F’ 1 Seed generation; n is less than or equal to 3;
(2-2) planting BC n F’ 1 Instead, after selfing, BC is harvested n F’ 2 Seed generation;
(2-3) planting BC n F’ 2 Instead, select and leave the restorer R 'with high resistant starch' 1 With sterile line A' 1 Paired test cross to obtain restorer R' 2 Seed;
(2-4) planting restorer line R' 2 The recovery line R Zhehui 7954 and the new hybrid rice variety II you 7954 are used as the contrast to observe the hybrid combination A' 2 ×R’ 2 Excellent hybridization combination with high selection and retention resistant starch and corresponding restorer line R' 3 With sterile line A' 3 Paired test cross to obtain restorer R' 4 Seed;
(2-5) planting restorer line R' 4 Identification of superior hybridization combinations A' 4 ×R’ 4 The yield and physical and chemical quality of (2) are selected and remained high-yield high-quality hybrid combination and corresponding restorer line R' 5 Obtaining a restorer line R' 6 Is a seed of (2);
3) Construction of sterile line A' 6 Restorer line R' 6 And preparing a seed breeding production system of the new variety of the high-resistance starch hybrid rice, and finally breeding the high-resistance starch hybrid rice.
2. The method for selectively breeding high resistant starch hybrid rice according to claim 1, wherein said high resistant starch is judged as having a resistant starch content of 10.0% or more.
3. The method for selectively breeding high-resistance starch hybrid rice according to claim 1, wherein in the step (1-4), the criterion for sterility is that the pollen abortion rate is not less than 99.99% and the bagging setting rate is not more than 0.01%.
4. The method for selectively breeding high-resistance starch hybrid rice according to claim 1, wherein in the step (2-5), the judgment criteria for high yield and high quality are: the yield is more than or equal to 550 kg/mu, and the amylose content of the physicochemical index is more than or equal to 30%.
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| CN103931483B (en) * | 2014-04-03 | 2015-10-07 | 上海市农业科学院 | The selection of high resistant starch content Japonica Hybrid CMS line and restorer and application |
| CN103960124B (en) * | 2014-05-15 | 2015-08-26 | 浙江杰农生物科技有限公司 | The method of the rice sterile line of resistant starch is rich in a kind of cultivation |
| AU2016272921A1 (en) * | 2015-06-05 | 2018-01-04 | International Rice Research Institute | Increasing hybrid seed production through higher outcrossing rate in cytoplasmic male sterile rice and related materials and methods |
| CN106900539B (en) * | 2017-02-14 | 2018-08-28 | 浙江大学 | A kind of method for breeding nonglutinous rice of high-resistance starch and the polymerization of high zinc |
| JP7239136B2 (en) * | 2018-07-25 | 2023-03-14 | 公立大学法人秋田県立大学 | Rice mutant with high resistant starch content, method for producing rice flour, method for producing resistant starch, method for producing rice gel, method for producing food, and method for producing mutant rice with high resistant starch content |
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