CN103891596B - A kind of high resistant starch content hybrid rice three hands over combination breeding and producing method for seed - Google Patents

Abstract

The invention discloses a kind of high resistant starch content hybrid rice three belonging to breeding and propagation technical field and hand over combination breeding and producing method for seed. The method arranges 4 numerous production of hybrid seeds gardens, is respectively used to rice sterile line A1 and maintenance be B1 breeding, high resistant starch content rice sterile line A2 and maintenance is that B2 breeding, heterozygous high resistant starch content rice sterile line A1/B2 breeding and high resistant starch content hybrid rice three hand over the combination A1/B2//R production of hybrid seeds. Can realizing high resistant starch content hybrid paddy rice three by the inventive method and hand over the stable production of hybrid seeds of combination, this three hands over combination to have the characteristic of high-resistance starch functional and high yield, high-quality, disease-resistant combination.

Description

A kind of high resistant starch content hybrid rice three hands over combination breeding and producing method for seed

Technical field

The invention belongs to breeding and propagation technical field, be specifically related to a kind of high resistant starch content hybrid rice three and hand over combination breeding and producing method for seed.

Background technology

According to the statistics of Ministry of Public Health, China's diabetics about 92,000,000 people in 2010, resistance to sugar measures low crowd especially up to 1.42 hundred million people. Rice is traditional staple food of China. The resistance starch content of common rice is too low, can digestible starch too high levels, it is easy to cause hyperglycemia. High resistant starch content rice variety selective is the key eating health.

High resistant starch content, rice special for diabetes breeding of new variety are the study hotspots currently implementing meals preventative strategies can. but be worth particularly it is to be noted that, domestic and international high resistant starch content rice pest insects lacks, only having indivedual research units reports such as Zhejiang University at present adopts induced-mutation technique to obtain high resistant starch content rice mutant, relevant sterile line of hybridized rice is also formed by the mutant hybridization transformation that genetic background is identical with Breeding for restoration lines number absolutely greatly, there is highly significant homogeneity and the outstanding problem of sibship too recent photo sound hybrid rice yield in each breeding material, its yield, disease resistance, rice varieties and cross combination that adaptability and level of quality and up-to-date incubation are promoted have a long way to go, cause that production cost is too high with the rice of exploitation, the industrialization hindering high resistant starch content function Oryza sativa L. advances. owing to high resistant starch content Functional rice breeding difficulty is big, science research input is far away from common high yield, high-quality and disease resisting rice breeding, in the urgent need to comprehensively utilizing the newest fruits of rice breeding, fast lifting high resistant starch content rice breeding level, better to promote the large-area applications of functional new varieties.

Being still had in the starting stage owing to the selection-breeding of high resistant starch content sterile line of hybridized rice works, the sterile line being bred as at present exists notable not enough in multifrequency natures such as flowering habit, disease resistance, quality and combining abilitys. Up-to-date excellent rice sterile line is relied on to cultivate heterozygous sterile line, it is possible to efficiently solve the selection-breeding of high resistant starch content rice sterile line and the production problem of breeding processed.

The selection-breeding of high resistant starch content hybrid rice and application, while being to maintain high-resistance starch functional characteristic, quickly take into account high yield, high-quality, disease resistance and adaptive key, it is needed for vast rice agriculture plantation high resistant starch content new rice variety realizes the reality increased income, also it is the basis of high resistant starch content Oryza sativa L. spread and industrialization, extremely important from the generation of source prevention China diabetes to taking meals strategy.

Summary of the invention

It is an object of the invention to provide a kind of high resistant starch content hybrid rice three and hand over combination breeding and producing method for seed.

A kind of high resistant starch content hybrid rice three hands over combination breeding and producing method for seed, it is characterised in that carry out in accordance with the following steps:

4 breedings and production of hybrid seeds garden are set, are respectively used to:

(1) rice sterile line Qianjiang 1A and maintenance are Qianjiang 1B breeding;

(2) high resistant starch content rice sterile line suitable sugar Xian 1A and maintenance system suitable sugar Xian 1B breeding;

(3) heterozygous high resistant starch content rice sterile line breeding, with rice sterile line Qianjiang 1A be maternal, high resistant starch content Oryza sativa L. keep system should sugar Xian 1B for male parent, preparation heterozygous high resistant starch content rice sterile line Qianjiang 1A/ should sugar Xian 1B;

(4) high resistant starch content paddy rice cross breeding three hands over the combination production of hybrid seeds, with heterozygous high resistant starch content rice sterile line Qianjiang 1A/ suitable sugar Xian 1B for maternal high resistant starch content rice restorer Zhejiang spoke 111 for male parent, Qianjiang 1A/ is combined in preparation high resistant starch content hybrid rice three friendship should sugar Xian 1B//Zhejiang spoke 111.

In the present invention, rice sterile line and maintenance system (A1, B1) can be: Qianjiang 1A, Qianjiang 1B or other close sterile lines and maintenance are; High resistant starch content rice sterile line with maintenance system (A2, B2) can be: suitable sugar Xian 1A, suitable sugared Xian 1B; High resistant starch content rice restorer (R) can be: Zhejiang spoke 111 or other close restorers.

According to high yield breeding technique isolation implant " Qianjiang 1A " and " Qianjiang 1B " (can be purchased from Zhejiang Nong Kezhong industry company limited) of rice sterile line " Qianjiang 1A ", breeding male sterile lines and maintenance are seed, according to the high yield breeding technique isolation implant " should sugar Xian 1A " of high resistant starch content rice sterile line " should sugar Xian 1A " and " should sugared Xian 1B ", (company cooperates free new rice variety with Zhejiang University, corresponding kind " suitable sugar Xian 1 " is protected to the application of Plant new variety protection office of the Ministry of Agriculture in December, 2013), breeding male sterile lines and maintenance are seed, high yield breeding technique isolation implant sterile line and maintenance system, production heterozygous male-sterile seed according to heterozygous high resistant starch content rice sterile line (" Qianjiang 1A/ suitable sugar Xian 1B "), the high-yield seeds production technique isolation implant heterozygous sterile line that mingles kind according to high resistant starch content hybrid rice three is (as " Qianjiang 1A/ should sugared Xian 1B " (can be purchased from our company in a large number or can be purchased from Zhejiang University on a small quantity) and high resistant starch content rice restorer " Zhejiang spoke 111 " (can be purchased from Zhejiang University, it it is the high resistant starch content rice varieties from extensive 7954 mutagenic and breedings in hybrid rice Elite restorer line Zhejiang, for applying for and authorizing new rice variety, application notification number: CNA004902E), cultivate or produce three friendship combination seeds (such as " Qianjiang 1A/ should sugar Xian 1B//Zhejiang spoke 111 " etc.), breeding restorer seed simultaneously. when adopting other close water, upland rice sterile line, maintenance system and restorer to carry out breeding processed, its technical method is similar with said process.

Feature according to the rice material that triple hybrid rice breeding links relates to; the supporting technologies such as different time sowing, field planting management, flowering time adjustment, artificial supplementary pollination, artificial roguing are provided; ensure that the various seed quality selecting system and hybrid thereof and purity reach the requirement of country's relevant regulations, it is achieved the large-scale production of each breeding system and hybrid seed.

Beneficial effects of the present invention: the present invention is that hybrid rice three of forming a complete production network mingles kind by four, compares general series of three-series hybrid rice breeding and the many links of producing method for seed, it is possible to achieve breeding that current high resistant starch content Oryza sativa L. is higher and hybrid seed yield; The hybrid rice three being bred as is handed over while being combined in maintenance high resistant starch content, has effectively taken into account high yield, high-quality, disease resistance and adaptability, it is possible to functional new varieties increasing both production and income is planted in the vast rice agriculture of better promotion; Science integration make use of the three line method breeding material of up-to-date high yield and high quality disease resisting rice new varieties and high resistant starch content functional rice; Heterozygous high resistant starch content rice sterile line compares with the high resistant starch content sterile line that isozygotys of current limited incubation, its Flowering Characteristics, disease resistance and level of quality are notable more excellent, being conducive to improving the yield of breeding and the production of hybrid seeds, hybrid rice three hands over combination hybrid yield significantly higher.

Accompanying drawing explanation

Fig. 1 is that high resistant starch content hybrid rice three hands over combination selection-breeding or breeding and seeding technique schematic diagram.

Detailed description of the invention

Below in conjunction with the drawings and specific embodiments, the present invention will be further described.

Embodiment 1

According to high yield breeding technique isolation implant " Qianjiang 1A " and " the Qianjiang 1B " of rice sterile line " Qianjiang 1A ", breeding male sterile lines and maintenance are seed; According to high yield breeding technique isolation implant " suitable sugar Xian 1A " and " the suitable sugar Xian 1B " of high resistant starch content rice sterile line " suitable sugar Xian 1A ", breeding male sterile lines and maintenance are seed; High yield breeding technique isolation implant sterile line and maintenance system, production heterozygous male-sterile seed according to heterozygous high resistant starch content rice sterile line (" Qianjiang 1A/ suitable sugar Xian 1B "); The high-yield seeds production technique isolation implant heterozygous sterile line planted is mingled (such as " Qianjiang 1A/ suitable sugar Xian 1B " and restorer " Zhejiang spoke 111 " according to high resistant starch content hybrid rice three, cultivate or produce three friendship combination seeds (such as " Qianjiang 1A/ should sugar Xian 1B//Zhejiang spoke 111 " etc.), breeding restorer seed simultaneously. When adopting other close water, upland rice sterile line, maintenance system and restorer to carry out breeding processed, its technical method is similar with said process.

Feature according to the rice material that triple hybrid rice breeding links relates to; the supporting technologies such as different time sowing, field planting management, flowering time adjustment, artificial supplementary pollination, artificial roguing are provided; ensure that the various seed quality selecting system and hybrid thereof and purity reach the requirement of country's relevant regulations, it is achieved the large-scale production of each breeding system and hybrid seed.

The high resistant starch content hybrid rice three being bred as mingles kind while keeping high resistant starch content, yield, quality and resistance significantly improve, colony separates little in maturation time and plant height etc., field management and final results will not be exerted an adverse impact, its population yield level is suitable with the yield level of " Zhejiang spoke 111 " hybridization with " Qianjiang 1A ", significantly higher with the yield of " Zhejiang spoke 111 " hybridization than " suitable sugar Xian 1A ", quality and resistance are notable more excellent, similar with producing the hybrid rice variety promoted mainly at present, under normal cultivation condition, every per mu yield is up to about 600 kilograms.

Claims (1)

1. a high resistant starch content hybrid rice three hands over combination breeding and producing method for seed, it is characterised in that carry out in accordance with the following steps:

4 breedings and production of hybrid seeds garden are set, are respectively used to:

(1) rice sterile line Qianjiang 1A and maintenance are Qianjiang 1B breeding;

(2) high resistant starch content rice sterile line suitable sugar Xian 1A and maintenance system suitable sugar Xian 1B breeding;

(3) heterozygous high resistant starch content rice sterile line breeding, with rice sterile line Qianjiang 1A be maternal, high resistant starch content Oryza sativa L. keep system should sugar Xian 1B for male parent, preparation heterozygous high resistant starch content rice sterile line Qianjiang 1A/ should sugar Xian 1B;

(4) high resistant starch content paddy rice cross breeding three hands over the combination production of hybrid seeds, with heterozygous high resistant starch content rice sterile line Qianjiang 1A/ suitable sugar Xian 1B for maternal high resistant starch content rice restorer Zhejiang spoke 111 for male parent, Qianjiang 1A/ is combined in preparation high resistant starch content hybrid rice three friendship should sugar Xian 1B//Zhejiang spoke 111.