CN103828712B - A kind of high resistant starch content hybrid rice three is handed over combination breeding method - Google Patents

Abstract

The invention discloses a kind of high resistant starch content hybrid rice three that belongs to crossbreeding technology field and hand over combination breeding method. The method is first taking rice sterile line A1 as female parent, high resistant starch content paddy rice maintainer B2 are as male parent, preparation heterozygous high resistant starch content rice sterile line A1/B2, taking heterozygous high resistant starch content rice sterile line A1/B2 as female parent, high resistant starch content rice restorer R are as male parent, preparation high resistant starch content hybrid paddy rice three is handed over combination A1/B2//R again. The high resistant starch content hybrid rice three being bred as by the inventive method hands over combination to have the characteristic of high-resistance starch functional and high yield, high-quality, disease-resistant combination.

Description

A kind of high resistant starch content hybrid rice three is handed over combination breeding method

Technical field

The invention belongs to crossbreeding technology field, be specifically related to a kind of high resistant starch content hybrid rice threeHand over combination breeding method.

Background technology

According to the statistics of the Ministry of Public Health, China diabetic approximately 9,200 ten thousand people in 2010, the low crowd of resistance to sugar amount is moreUp to 1.42 hundred million people. Rice is traditional staple food of China. The resistance starch content of common rice is too low, canDigestible starch too high levels, easily causes hyperglycaemia. High resistant starch content rice variety selective be eat strongThe key of health.

High resistant starch content, rice special for diabetes breeding of new variety are current enforcement meals preventative strategiesStudy hotspot. But be worth particularly noting, high resistant starch content paddy rice resource shortage both at home and abroad, at presentIt is prominent that indivedual research units such as Jin You Zhejiang University report adopts induced-mutation technique to obtain high resistant starch content paddy riceVariant, relevant sterile line of hybridized rice is also the sudden change identical by genetic background with the absolutely large number of restorer seed selectionBody hybridization transformation forms, and each breeding material exists extremely significant homogeneity and affiliation, and too recent photo sound is assortedHand over the outstanding problem of rice yield, its output, disease resistance, adaptability and level of quality and up-to-date incubation are promotedRice varieties and cross combination have a long way to go, cause the rice of production cost and exploitation too high, hindered heightThe industrialization of resistance starch content function paddy rice advances. Due to high resistant starch content Functional rice breeding difficultyDegree is large, scientific research drops into far away from common high yield, high-quality and disease resisting rice breeding, in the urgent need to comprehensive utilization waterThe newest fruits of rice breeding, fast lifting high resistant starch content rice breeding level, better to promote functionThe large-area applications of property new varieties.

Because the seed selection work of high resistant starch content sterile line of hybridized rice was still had in the starting stage, at presentSterile line through being bred as exists significantly not enough in multifrequency natures such as flowering habit, disease resistance, quality and coordinate force.Rely on up-to-date good rice sterile line to cultivate heterozygous sterile line, can effectively solve high-resistance starch and containThe amount seed selection of rice sterile line and the production problem of breeding processed.

The Breeding and application of high resistant starch content hybrid rice is keep high-resistance starch functional characteristic sameTime, take into account fast high yield, high-quality, disease resistance and adaptive key, be that high resistance shallow lake is planted in vast rice agricultureIt is required that powder content new rice variety is realized the reality increasing income, and is also high resistant starch content paddy rice spreadWith the basis of industrialization, extremely important from the generation of source prevention China diabetes to taking meals strategy.

Summary of the invention

The object of the present invention is to provide a kind of high resistant starch content hybrid rice three to hand over combination breeding method,Cultivate the rice varieties of high resistant starch content.

The vegetable material the present invention relates to comprises: 1) high yield, high-quality, Resistant, adaptability, coordinate force are strongRice sterile line (A1, as Qianjiang 1A); 2) have high resistant starch content paddy rice maintainer (B2,As suitable sugared Xian 1B); 3) good, the obvious high resistant starch content restorer of hybrid vigour of coordinate force (R,As Zhejiang spoke 111).

A kind of high resistant starch content hybrid rice three is handed over combination breeding method, carries out in accordance with the following steps:

(1) taking rice sterile line Qianjiang 1A as maternal, the suitable sugared Xian 1B of high resistant starch content paddy rice maintainerFor male parent, the suitable sugared Xian 1B of preparation heterozygous high resistant starch content sterile line Qianjiang 1A/;

(2) taking the suitable sugared Xian 1B of heterozygous high resistant starch content rice sterile line Qianjiang 1A/ as maternal, heightResistance starch content rice restorer Zhejiang spoke 111 is male parent, and preparation high resistant starch content hybrid rice three is handed overCombination Qianjiang 1A/ should sugared Xian 1B//Zhejiang spoke 111.

By to breeding with production of hybrid seeds process with the plant height, the flowering habit that relate in hybrid variety comparative test, becomeThe overall assessment of ripe time, sterile gene and multiple characteristics such as recovery capability, hybrid vigour, seed selection A1,B2 and R system can match each other, and three mingle kind of the hybridization of the high resistant starch content with actual application valuePaddy rice newly combines. Specifically including, but not limited to plant height and maturation time phase that 1) A1 system and B2 areClosely, B2 system keeps the sterile gene of A1 system completely, A1/B2 hybrid to have three be sterile line standard of perfectionSterile line index; 2) sterile line A1 and heterozygous sterile line A1/B2 have high outcrossing seed-setting rate and breedingOutput with the production of hybrid seeds; 3) A1, B2 and R cording have high-combining ability, and three mingle kind of an A1/B2//R has strong assortedThe advantage of kind, the levels such as its output, quality and resistance are suitable with the upper spread kind of production.

Beneficial effect of the present invention: the hybrid rice three that 1) the present invention is bred as is handed over and is combined in maintenance high-resistance starchWhen content, effectively take into account high yield, high-quality, disease resistance and adaptability, can better promote vast riceFunctional new varieties increasing both production and income is planted in agriculture. 2) science integration has utilized up-to-date high yield and high quality disease resisting rice newThe three line method breeding material of kind and high resistant starch content functional rice. 3) heterozygous high-resistance starch containsThe high resistant starch content sterile line comparison of isozygotying of amount rice sterile line and current limited incubation, its Flowering Characteristics,Disease resistance and adaptation level are significantly more excellent, are conducive to improve the output of breeding and the production of hybrid seeds, and hybrid rice three is handed overCombination hybrid yield is significantly higher.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention will be further described.

Embodiment 1

The present embodiment is so that " " (can be purchased from Zhejiang Nong Kezhong industry Co., Ltd) is as rice sterile line for Qianjiang 1A(A1), " should sugared Xian 1B " (rice varieties that company is free, corresponding kind No. 1, sugared Xian " should "In December, 2013 to the application protection of new variety of plant protection office of the Ministry of Agriculture) be high resistant starch content(can be purchased from Zhejiang University, be from the good recovery of hybrid rice for paddy rice maintainer (B2), " Zhejiang spoke 111 "Be the high resistant starch content rice varieties of extensive 7954 mutagenic and breedings in Zhejiang, for applying for and authorize new rice variety,Application notification number: CNA004902E) be high resistant starch content rice restorer (R).

Taking " Qianjiang 1A " as maternal, be male parent with " should sugared Xian 1B ", production heterozygous high resistant starch content(i.e. " Qianjiang 1A/ should sugared Xian 1B ") is complete through checking this hybrid to have sterile line characteristic for rice sterile line, and 100%Sterile, meet the requirement of sterile line in hybrid rice production.

Sterile with above-mentioned heterozygous is maternal, and taking " Zhejiang spoke 111 " as male parent, combination (i.e. " money is handed in preparation threeRiver 1A/ should sugared Xian 1B//Zhejiang spoke 111 ").

The feature of the rice material relating to according to triple hybrid rice breeding links, while providing differenceBetween the supporting technology such as sowing, field planting management, flowering time adjusting, artificial supplementary pollination, artificial impurity elimination,Ensure that the seed quality of various choosings system and hybrid thereof and purity reach the requirement of national relevant regulations, realize eachThe large-scale production of breeding system and hybrid seed.

Through land for growing field crops output associating variety comparative test, show that three friendship combination hybrid plant in the time of field planting of seed selection are highShortly slightly separate with maturation time, but range of variation can obviously not affect output and harvest time, its colony producesWater gaging is flat suitable with the yield level of " Zhejiang spoke 111 " hybridization with " Qianjiang 1A ", than " should sugared Xian 1A " with "Zhejiang spoke 111 " it is high that significantly wants, quality and resistance are significantly more excellent, promotes mainly with current production by the output of hybridizationHybrid rice variety is similar, and under normal cultivation condition, every per mu yield can reach 600 kilograms of left and right.

Claims (1)

1. high resistant starch content hybrid rice three is handed over a combination breeding method, it is characterized in that, according to asLower step is carried out:

(1) taking rice sterile line Qianjiang 1A as maternal, the suitable sugared Xian 1B of high resistant starch content paddy rice maintainerFor male parent, the suitable sugared Xian 1B of preparation heterozygous high resistant starch content sterile line Qianjiang 1A/;

(2) taking the suitable sugared Xian 1B of heterozygous high resistant starch content rice sterile line Qianjiang 1A/ as maternal, heightResistance starch content rice restorer Zhejiang spoke 111 is male parent, and preparation high resistant starch content hybrid rice three is handed overCombination Qianjiang 1A/ should sugared Xian 1B//Zhejiang spoke 111.