CN103430824A - Two-line hybrid rice seed breeding method - Google Patents
Two-line hybrid rice seed breeding method Download PDFInfo
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- CN103430824A CN103430824A CN2013102420417A CN201310242041A CN103430824A CN 103430824 A CN103430824 A CN 103430824A CN 2013102420417 A CN2013102420417 A CN 2013102420417A CN 201310242041 A CN201310242041 A CN 201310242041A CN 103430824 A CN103430824 A CN 103430824A
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Abstract
The present invention discloses a two-line hybrid rice seed Yanliangyou 2298 breeding method, and belongs to the field of rice hybrid seed production. According to the present invention, an indica type photo-thermosensitive genic male sterile line Yan 229S is adopted as a female parent, and a two-line restorer line Yanhui 888 is adopted as a male parent to produce the two-line hybrid rice seed Yanliangyou 2298, wherein the preservation number of the Yan 229S is CGMCCNO.7660; and the hybrid rice seed has characteristics of early mature stage, high yield, stable yield, national standard grade III good quality rice, and strong disease resistance.
Description
Technical field
The present invention relates to paddy rice cross breeding seed production field, in particular a kind of selection of double-linear hybrid rice seeds salt two excellent 2298.
Background technology
Paddy rice is the staple food crop of China, and the cultivated area of hybrid rice accounts for the over half of China's Monitoring of Paddy Rice Plant Area.The improvement of double-linear hybrid rice kind is the major fields of China's agri-scientific research in the recent period, and high-quality, high yield, many anti-, wide fitting are the important long-term targets of rice breeding.It is one of important channel of realizing China's breeding of hybridized rice third phase target that the ideotype that Academician YUAN Long-ping proposes and hybrid vigour combine.
Nearly ten years, China's breeding of hybridized rice research has obtained the achievement that makes the world attract attention, in succession being bred as the super double-linear hybrid rices such as " two line system ", " Liangyou0293 ", " excellent No. 2 of Y two " newly combines, and realizing China's super rice target of hundred mu of first, second and third phases of high yield side's per mu yield, sign China's rice breeding and production continue to keep world lead level.China's super hybrid rice realizes that the breeding objective of first, second and third phase is also the yield level in hundred mu of sides and thousand mu of high yield sides, and the yield level that China's paddy rice large tracts of land is produced has just realized that per mu yield exceedes 500 kilograms, average yield per mu does not also reach 550 kilograms of levels.At present, super double-linear hybrid rice new varieties ubiquity high yield and inharmonic defects such as resistance, high yield and high-quality of application in production.Seed selection, production and popularization rice quality reach national fine quality rice standard, stable high yield, the ripe phase is moderate, disease resistance is strong new hybrid rice varieties is the effective way that further improves China's hybrid rice production output, ensures national food safety.
Summary of the invention
The object of the invention is to for ensureing that China's grain-production reaches safely the double-linear hybrid rice that China's Rice Production market safety cultivates and newly combines, and a kind of selection that obtains the double-linear hybrid rice seeds of high yield is provided
A kind of selection of double-linear hybrid rice seeds salt two excellent 2298, select indica type photo-thermo-sensitive genic male sterile line " salt 229S " for maternal, and take indica type two is that restorer " salt extensive 888 " be male parent, production double-linear hybrid rice seeds " salt two excellent 2298 "; Described salt 229S deposit number: CGMCC NO.7660.
Described selection, the space length >=500m of safety isolation, or around time isolation, 500m is separated by more than 15d with the heading flowering period of described father, female parent with the heading flowering period of the rice varieties of interior plantation.
Described selection, male parent is divided two phases sowings, the more maternal early sowing 38～40d of the 1st phase male parent, or leaf age differs from 6.8～7.0 leaves, two male parent dates of seeding phase, differed 10～12d.
Described selection, production of hybrid seeds old farmer, maternal row are planted with period interval than 2: 12～14, two phase male parent duplicate rows, male parent distance between rows and hills 30cm * 15cm; Maternal distance between rows and hills 16.7cm * 13.3cm, 2～3, every cave, plant sufficient Basic Seedling.
Described selection, the total consumption 35～40g/ mu of seed farm " 920 ", divide and spray for 3 times, cuts leaf for the first time when female parent heading 5-10% and sprayed 15g/ mu the same day, cuts the leaf second day and spray 15～20g/ mu, cuts leaf and within the 4th day, spray 5～10g/ mu.
Described selection, be shown in that fringe phase, fringe phase beginning, full heading time prevent and treat rice kernel smut 3～4 times continuously.
Described selection, the paddy rice production of hybrid seeds will be prevented assorted pure keeping the time of infertility, emphasis is the strict impurity elimination of heading flowering period 3～4 times, pulls out and father, maternal discrepant plant.Contain bow structure bundle 25～30d, first by the clean rear results hybrid seed of male parent harvesting.
The present invention utilizes indica type photo-thermo-sensitive genic male sterile line " salt 229S " for maternal, take indica type two be restorer " salt extensive 888 " for two of male parent production be that feature and the beneficial effect of hybrid rice seed " salt two excellent 2298 " is as follows:
Double-linear hybrid rice " the salt two excellent 2298 " stable high yield that the present invention produces: multiple spot variety comparative test in the Jiangsu Province of participation our company tissue in 2009,572.9 kilograms of average yields, than excellent 084 volume increase 5.16% of contrast II, reach utmost point significance level.Multiple spot variety comparative test in the Jiangsu Province of participation our company tissue in 2011,592.8 kilograms of average yields, than excellent 084 volume increase 5.41% of contrast II, reach utmost point significance level.Multiple spot great Qu demonstration test in the Jiangsu Province of participation our company tissue in 2012,626.60 kilograms of average yield per mus, than excellent 084 volume increase 8.15% of contrast II.See attached list 1.
Double-linear hybrid rice " the salt two excellent 2298 " rice quality that the present invention produces is excellent: 2011 according to Ministry of Agriculture's rice and quality of item supervision and inspection center test (Wuhan: Hua Zhong Agriculture University): brown rice rate 81.4%, polished rice rate 70.5%, head rice rate 66.9%, grain length 6.8mm, aspect ratio 3.1, the white rate 30% of chalk, chalkiness degree 2.7%, 1 grade of transparency, disappear 6.0 grades of values of alkali, glue denseness 81mm, amylose content 16.5%, the all indexs of rice quality all reach three grades of superior rice standards of GB, good palatability.See attached list 2.
Double-linear hybrid rice " the salt two excellent 2298 " disease resistance that the present invention produces is strong: 2011 according to Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province inoculated identification result: middle bacterial leaf spot resistant JS49-6 pathogenic strain (3 grades), middle sense bacterial leaf spot KS-6-6 pathogenic strain (5 grades), middle bacterial leaf spot resistant Zhejiang 173 pathogenic strains (3 grades), in anti-PX079 pathogenic strain (3 grades), to all immunity of 6 biological strains such as rice blast ZB10, ZF1, ZC15, ZD7, ZE3, ZG1, sick level is 0 grade, anti-panicle blast (1 grade), sense banded sclerotial blight (S), anti-stripe virus disease (R).See attached list 3.
Double-linear hybrid rice " the salt two excellent 2298 " economical character that the present invention produces is good: the time of infertility 143d, than contrast II excellent 084 Zao 1d, ripe middle Xian type in genus.Plant height 125cm left and right, plant type is collecting and distributing moderate, and stooling is stronger, and the percentage of earbearing tiller is high, and general mu is fringe 15.3 ten thousand effectively, 185 left and right of every total grain panicle, ripening rate 85% left and right, thousand kernel weight 27.5g left and right, slightly short awns.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1: the selection of hybrid rice of the present invention " salt two excellent 2298 " seed.
Select indica type photo-thermo-sensitive genic male sterile line " salt 229S " for maternal, take indica type two is that restorer " salt extensive 888 " be male parent, production double-linear hybrid rice seeds " salt two excellent 2298 ".Described salt 229S has been deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 29th, 2013, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address, deposit number: CGMCC NO.7660, Classification And Nomenclature paddy rice (Oryza sativa).Salt extensive 888 is bred as for Jiangsu Inst. of Coastal Area Agricultural Sciences, just, in large tracts of land production of hybrid seeds application, can buy by market approach.Concrete selection is as follows:
(1) safety isolation: space length >=500m, or around time isolation, 500m is separated by more than 15d with the heading flowering period of described father, female parent with the heading flowering period of the rice varieties of interior plantation.
(2) begin sowing in good time: male parent is divided two phases sowings, the more maternal early sowing 38～40d of the 1st phase male parent, or leaf age differs from 6.8～7.0 leaves, and two male parent dates of seeding phase, differed 10～12d.
(3) rational close planting, suitable row ratio.Production of hybrid seeds old farmer, maternal row are planted with period interval than 2: 12～14, two phase male parent duplicate rows, male parent distance between rows and hills 30cm * 15cm; Maternal distance between rows and hills 16.7cm * 13.3cm.
(4) use " 920 " technology and artificial supplementary pollination.The total consumption 35～40g/ mu of seed farm " 920 ", divide and spray for 3 times, cuts leaf for the first time when female parent heading 5-10% and sprayed 15g/ mu the same day, cuts the leaf second day and spray 15～20g/ mu, cuts leaf and within the 4th day, spray 5～10g/ mu.From fringe phase beginning to neat fringe, catch up with pollen to carry out artificial supplementary pollination 5-6 time whole flowering stage every day at florescence.
(5) prevent and treat the kernel smut technology: cut phase, fringe phase beginning and for full heading time bactericide " go out black No. 1 " (producer's medicine) prevent and treat continuously kernel smut 3～4 times.
(6) pure technology is gone in impurity elimination: the paddy rice production of hybrid seeds will be prevented assorted pure keeping the whole time of infertility from the rice seedling bed to the land for growing field crops, and emphasis is the strict impurity elimination of heading flowering period 3～4 times, pulls out form and the discrepant plant of maternal " salt 229S " and male parent " salt extensive 88 ".Contain bow structure bundle 25～30d, first by the clean rear results hybrid seed of male parent harvesting.
Embodiment 2: the concrete Breeding Process of hybrid rice seed of the present invention " salt two excellent 2298 ".
The hybrid rice seeds that the present invention produces " salt two excellent 2298 ", in the New Hybrid Rice Combination variety comparative test He great district demonstration of 2009～2012 years participation our unit tissues, shows stable high yield, and average yield per mu is than contrasting excellent 084 volume increase 5% left and right of II.In Table 1.
Table 1: the excellent 2298 variety comparative test output situations of different annual salt two
The hybrid rice seeds that the present invention produces " salt two excellent 2298 ", within 2011, according to the test of Ministry of Agriculture's rice and quality of item supervision and inspection center, (Wuhan: Hua Zhong Agriculture University), all indexs of its rice quality all reach GB superior rice standard more than three grades.In Table 2.
Table 2:2011 salt two excellent 2298 rice quality indexs
The hybrid rice seeds that the present invention produces " salt two excellent 2298 ", 2009 according to Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province inoculated identification result: middle bacterial leaf spot resistant JS49-6 pathogenic strain (3 grades), middle bacterial leaf spot resistant KS-6-6 pathogenic strain (3 grades), middle bacterial leaf spot resistant Zhejiang 173 pathogenic strains (3 grades), in anti-PX079 pathogenic strain (3 grades), to all immunity of 6 biological strains such as rice blast ZB, ZF, ZC, ZD, ZE, ZG, sick level is 0 grade, anti-panicle blast (1 grade), high resistance banded sclerotial blight (HR), the disease resistance indices is better than check variety II excellent 084.In Table 3.
Table 3:2009 salt two excellent 2298 Disease Resistance Identification results
Embodiment 3: hybrid rice of the present invention " salt two excellent 2298 " high-yield culture technique
(1) proper seeding, cultivate strong seedling: according to various places Rice Production custom, reasonable arrangement sowing time, guarantee safety heading flowering.10～15 kilograms of every mu of clean seedling plate amounts of broadcasting of water-raised seedling, 20～25 kilograms of every mu of clean seedling plate amounts of broadcasting of non-irrigated seedling, 1.0～1.5 kg/acres of land for growing field crops sowing quantities.1.5～2.0 kilograms of every mu of land for growing field crops sowing quantities of transplanting.Rice seedling bed will use sufficient base manure, early executes wean fertile, imposes long thick short fetilizer for tillering, send and transfer fertilizer, and carry out rice seedling bed disease pest and weed control, cultivates the strong seedling of of the right age many tillers.
(2) transplant rational close planting: transplant, rational close planting is the basis of high-yield culturing in good time in good time.At 30～35 days water-raised seedling length of time rice seedlings grow, the non-irrigated seedling length of time rice seedlings grow was advisable with 20～25 days, general 18～20 days of the transplanting length of time rice seedlings grow.In upper fertility field plant soon specification 1.6～1.8 ten thousand caves/mu left and right, 1～2, every cave, plant sufficient Basic Seedling 6～80,000/mu, it is 1.8～2.0 ten thousand caves/mu that the field that fertility is low will suitably increase Planting Densities, accomplishes shallow slotting, even cultivation while planting, and guarantees to plant quality.
(3) scientific utilization of fertilizer, short seedling is early sent out: scientific utilization of fertilizer, it is the key that forms yielding Populations that short seedling is early sent out.The above cultivation of per mu yield 650kg kilogram, general fertilizer requirement amounts to purity nitrogen 20 kg/acres of left and right, and phosphorus, potash fertilizer are used in collocation, phosphorus, potash fertilizer are main as base manure, and fertilising should be adhered to " front short, in steady, rear benefit ", principle, base manure accounts for 60% left and right, and tillering fertilizer accounts for 15% left and right, and fringe granulated fertilizer accounts for 25% left and right.
(4) run water slurry well, coordinate colony: water starch management should take that shallow water transplants seedlings, very little water is lived, thin moisture tiller, deep water heading flowering, the irrigation method of later stage alternation of wetting and drying.Require to plant latter about 20 days, send out foot and wait the fringe seedling, field is put in draining in good time, controls peak seedling and is no more than 250,000/mu, finally becomes fringe 16～170,000/mu, and moistening endowment of later stage, gather in and cut off the water supply in first 5 days, guarantees the blue or green seed Huang of stalk.
(5) the anti-worm of curing the disease, keep a full stand of seedings and increase fringe: broadcast front with medicament seed soaking and prevent and treat the seed-borne diseases such as bakanae disease and Aplelenchiodes bessegi of rice.Seeding stage attention control small brown rice planthopper, rice thrips, aphid, field period will be carried out the preventing and controlling of the damage by disease and insect such as control rice blast, bacterial leaf-blight, banded sclerotial blight, black streak dwarf, snout moth's larva, brown plant-hopper, guarantees that the rice seedling normal growth grows, and keeps a full stand of seedings to strive fringe and strive for high yield.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (7)
1. the selection of a double-linear hybrid rice seeds salt two excellent 2298, it is characterized in that, select indica type photo-thermo-sensitive genic male sterile line " salt 229S " for maternal, take indica type two is that restorer " salt extensive 888 " be male parent, production double-linear hybrid rice seeds " salt two excellent 2298 "; Described salt 229S deposit number: CGMCC NO.7660.
2. selection according to claim 1, is characterized in that, the space length >=500m of safety isolation, or around time isolation, 500m is separated by more than 15d with the heading flowering period of described father, female parent with the heading flowering period of the rice varieties of interior plantation.
3. selection according to claim 1, is characterized in that, male parent is divided two phases sowings, the more maternal early sowing 38～40d of the 1st phase male parent, or leaf age differs from 6.8～7.0 leaves, and two male parent dates of seeding phase, differed 10～12d.
4. selection according to claim 1, is characterized in that, production of hybrid seeds old farmer, maternal row are planted with period interval than 2: 12～14, two phase male parent duplicate rows, male parent distance between rows and hills 30cm * 15cm; Maternal distance between rows and hills 16.7cm * 13.3cm, 2～3, every cave, plant sufficient Basic Seedling.
5. selection according to claim 1, is characterized in that, the total consumption 35～40g/ mu of seed farm " 920 ", divide and spray for 3 times, cut leaf for the first time when female parent heading 5-10% and sprayed 15g/ mu the same day, cut the leaf second day and spray 15～20g/ mu, cut leaf and within the 4th day, spray 5～10g/ mu.
6. selection according to claim 1, is characterized in that, seeing that fringe phase, fringe phase beginning, full heading time prevent and treat rice kernel smut 3～4 times continuously.
7. selection according to claim 1, is characterized in that, the paddy rice production of hybrid seeds will be prevented assorted pure keeping the time of infertility, and emphasis is the strict impurity elimination of heading flowering period 3～4 times, pulls out and father, maternal discrepant plant.Contain bow structure bundle 25～30d, first by the clean rear results hybrid seed of male parent harvesting.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931487A (en) * | 2014-05-09 | 2014-07-23 | 湖北亿金农业科技开发有限公司 | Seed production method for bilinear hybrid rice overslipping flowering time |
| CN104585025A (en) * | 2015-02-03 | 2015-05-06 | 盐城明天种业科技有限公司 | Method for breeding broad-spectrum rice restorer line hybrid rice combination |
| CN106069722A (en) * | 2016-06-26 | 2016-11-09 | 合肥慧谷农业科技有限公司 | A kind of colored time do not meet two is the producing method for seed of paddy rice cross breeding |
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2013
- 2013-06-19 CN CN2013102420417A patent/CN103430824A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931487A (en) * | 2014-05-09 | 2014-07-23 | 湖北亿金农业科技开发有限公司 | Seed production method for bilinear hybrid rice overslipping flowering time |
| CN104585025A (en) * | 2015-02-03 | 2015-05-06 | 盐城明天种业科技有限公司 | Method for breeding broad-spectrum rice restorer line hybrid rice combination |
| CN104585025B (en) * | 2015-02-03 | 2017-04-19 | 盐城明天种业科技有限公司 | Method for breeding broad-spectrum rice restorer line hybrid rice combination |
| CN106069722A (en) * | 2016-06-26 | 2016-11-09 | 合肥慧谷农业科技有限公司 | A kind of colored time do not meet two is the producing method for seed of paddy rice cross breeding |
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Application publication date: 20131211 |