CN104429923B - High resistant starch content japonica rice breeding method - Google Patents
High resistant starch content japonica rice breeding method Download PDFInfo
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- CN104429923B CN104429923B CN201410586260.1A CN201410586260A CN104429923B CN 104429923 B CN104429923 B CN 104429923B CN 201410586260 A CN201410586260 A CN 201410586260A CN 104429923 B CN104429923 B CN 104429923B
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Abstract
The invention discloses a high resistant starch content japonica rice breeding method belonging to the technical field of crossbreeding. The method comprises the following steps: taking high resistant starch content indica type rice as a parent, hybridizing with low-medium straight chain starch content quality japonica rice to obtain F1 generation seeds, selfing the F1 generation seeds, selecting japonica rice type rice single train in F2 generation plants, firstly screening the rice plant with little chalky, and then analyzing easily screened medium straight chain starch content rice plant, and finally analyzing and selecting the rice plant with resistant starch content, selecting excellent single strain with little chalky, proper resistance and straight chain starch content in the F3 generation strain system, and detecting starch rice physicochemical quality in the F4 generation strain system, selecting the strain system in low gelatinization point and soft gel consistency, repeatedly selfing, launching system breeding to obtain high-quality rice with high resistant starch, medium straight chain starch and good physicochemical quality. The high-quality japonica rice bred by the invention can meet the selection of the japonica rice consumer while maintaining the high resistant starch function characteristic.
Description
Technical field
The invention belongs to crossbreeding technology field, and in particular to a kind of high-quality high resistant starch content breeding nonglutinous rice side
Method.
Background technology
According to the statistics of Ministry of Public Health, about 92,000,000 people of China's diabeticss in 2010, resistance to sugar amount low crowd be more up to
1.42 hundred million people.Rice is traditional staple food of China.The resistance starch content of common rice is too low, can digestible starch too high levels,
It is easily caused hyperglycemia.High resistant starch content rice variety selective is the key for eating health.
High resistant starch content, rice special for diabetes breeding of new variety are the research heat for currently implementing meals preventative strategies can
Point.But it is worth particularly it is noted that domestic and international high resistant starch content rice pest insects shortage, only Zhejiang University etc. is indivedual at present
Research unit's report obtains high resistant starch content long-grained nonglutinous rice mutant using induced-mutation technique.Resistance in Different Types of Rice is formed sediment
Powder content significant difference, rice variety resource resistance starch content are of a relatively high, and high also many below 1%, Jing rice is lower, high
Only 0.5%, high-quality Semen oryzae sativae in market is 0.1%～0.3%.
With the increasingly raising of the development and living standard of the urbanization of China, to the selection trend of Jing rice rice increasingly
Substantially.Therefore, high-resistance starch Jing rice varieties selection-breeding is also needing when business.
The selection-breeding and application of high resistant starch content high quality japonica, it is while being to maintain high-resistance starch functional characteristic, full
The selection of sufficient Jing rice rice consumer, takes meals strategy from source to high resistant starch content Oryza sativa L. spread and industrialization
The generation of head prevention China diabetes is significant.
The content of the invention
It is an object of the invention to provide a kind of high-quality high resistant starch content method for breeding nonglutinous rice, the high quality japonica of cultivation
While keeping high-resistance starch functional characteristic, the selection of Jing rice rice consumer is met.
A kind of high resistant starch content method for breeding nonglutinous rice, is carried out in accordance with the following steps：
(1) with high resistant starch content long-grained nonglutinous rice as parent, hybridize with low amylose content high quality japonica, hybridization obtains F1
For seed；
(2) F1 generation selfing, obtains F2 for seed；
(3) Jing rice type Oryza sativa L. is selected in colony in F2, the Oryza sativa L. plant that setting percentage reaches more than 90% is harvested by individual plant
Strain, first selects rice plant of the chalkiness degree less than 10%, then analyzes the water that the easy amylose content for determining is 17%～20%
Rice plants, post analysis select the rice plant of high resistant starch content more than 8%；
(4) Jing rice type Oryza sativa L. is selected in strain in F3, the Oryza sativa L. plant that setting percentage reaches more than 90% is harvested by individual plant
Strain, analysis selects chalkiness degree less than 10%, amylose content 17%～20% and high resistant starch content more than 8% it is excellent
Individual plant；
(5) in F4 is for strain, by single-strain planting, the individual plant of high yield, mixed strain of receiving is selected to carry out rice physical and chemical quality inspection
Survey, caustic SCC is selected higher than 5 grades, the strain of more than 60 millimeters of flexible glue denseness；
(6), in F5～F7 self progenies, repeatedly the step of (5), systematic breeding, low amylose content in acquisition are carried out
15%～20%, caustic SCC is higher than 5 grades, the high-quality high resistant starch content Jing rice of more than 60 millimeters of flexible glue denseness.
In described breeding material, the long-grained nonglutinous rice of high amylose content is Zhejiang spoke 111, middle low amylose content high-quality Jing
Rice parent is elegant water 11.
In breeding material of the present invention, the parent of high resistant starch content has applied authorizing guarantor for Zhejiang University
The long-grained nonglutinous rice new varieties " Zhejiang spoke 111 " of shield.The Resistance of Rice Varieties content of starch 10%, amylose content more than 30%, ebonite
Denseness (30 millimeters), in gelatinization point, Appearance Quality of Paddy Rice is poor, and (chalkness ratio 90%, 40%), cooking quality is poor for chalkiness degree.
The low amylose content high quality japonica parent for being adopted is elegant water 11.Elegant water 11 is by the selection-breeding of Jiaxing City academy of agricultural sciences
Conventional high quality japonica kind (authorization certificate：2008021) state examines rice；It is low (16.3%) in the rice varieties amylose content,
Flexible glue denseness (71 millimeters), low gelatinization point, Appearance Quality of Paddy Rice it is excellent (chalky grain rate 4%, chalkiness degree 0.2%), cooking quality
It is excellent.
From long-grained nonglutinous rice Zhejiang spoke 111 be engaged with Jing rice shows water 11 can selection-breeding obtain resistant starch and amylose content with
The high-grade rice kind that characteristic is taken into account.The kind amylose content 19%, 61 millimeters of gel consistence, between two parents,
Low gelatinization point, Appearance Quality of Paddy Rice is more excellent, and (chalky grain rate 15%, 5%), cooking quality is excellent for chalkiness degree.
In F5～F7 self progenies, individual plant is selected to carry out the systematic breeding of target starch property and economical character, excellent middle choosing
It is excellent, 2～3 generation of general selection-breeding.
Described high-quality is rice physicochemical property, including medium amylase content, low gelatinization point and flexible glue denseness are joined
According to High quality rice rice standard NY122-86.
Beneficial effects of the present invention：The present invention takes high-resistance starch long-grained nonglutinous rice and the hybridization of middle low amylose high quality japonica,
Selection-breeding high-resistance starch, medium amylose, low gelatinization point, flexible glue denseness take into account the high-grade rice kind of parents, specific aim
By force, it is that high-quality character is effectively polymerized.While the high quality japonica of cultivation keeps high-resistance starch functional characteristic, Jing rice rice is met
The selection of consumer.Meals strategy is taken from source prevention China to high resistant starch content Oryza sativa L. spread and industrialization
The generation of diabetes is significant.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1
Breeding material：
Zhejiang spoke 111：(Ministry of Agriculture authorizes Plant variety right, Shen to the long-grained nonglutinous rice new varieties of the high-resistance starch of Zhejiang University's selection-breeding
Number 20080431.6) please.
Elegant water 11：High quality japonica kind (the authorization certificate of Jiaxing City academy of agricultural sciences selection-breeding：2008021) state examines rice.High-quality is high
The Breeding Process of resistant starch Oryza sativa L. is：
(1) 2008 year spring, Hainan Lingshui with high resistant starch content long-grained nonglutinous rice " Zhejiang spoke 111 " as male parent, the low straight chain with
Content of starch high quality japonica " elegant water 11 " hybridizes to obtain 19, F1 generation seed；
(2) 2008 year summer, 18 plants of Zhejiang Hangzhou plantation F1 plant, the ripe mixed F2 for receiving selfing is for more than 8000, seed；
(2) 2009 year summer, Zhejiang Hangzhou select more than 1500 plants of Jing rice type rice plant, by list in F2 in colony
Strain harvests setting percentage and reaches more than 90%, and (chalky grain rate is less than 20%, and chalkiness degree is less than first to select chalk to lack more than 689 plants in vain
10%) 216 plants of the individual plant of amylose content medium (content is 17%～20%), is determined, resistance starch content (content is analyzed
More than 8%), it is selected in 92 plants of rice plant；
(4) 2010 year summer, Zhejiang Hangzhou select Jing rice type Oryza sativa L. in F3 in strain, harvest by individual plant, analysis choosing
Select chalk it is few in vain (chalky grain rate less than 20%, chalkiness degree less than 10%), resistance starch content (more than 8%) and amylose content
(17%～20%) moderate fine individual plant taken into account 46；
(5) in F4 is for strain, by single-strain planting, the individual plant of high yield, mixed strain of receiving is selected to carry out rice physical and chemical quality inspection
Survey, select low gelatinization point (caustic SCC is higher than 5 grades), the strain 21 of flexible glue denseness (more than 60 millimeters)；
(6), in F5～F7 self progenies, repeatedly the step of (5), systematic breeding, low amylose content in acquisition are carried out
(15%～20%), low gelatinization point (caustic SCC is higher than 5 grades), the high-quality high-resistance starch of flexible glue denseness (more than 60 millimeters) contain
Amount Jing rice 1, names the sugared Jing 1 that is advisable.
No. 1 main feature of suitable sugared Jing of above-mentioned steps selection-breeding shows as：Contain with high amylose starchess and middle low amylose
The parents of amount compare, it will be apparent that distinguishing character is：Preferably sugar Jing 1 is Jing rice types, high-resistance starch (8%) and amylose
Content medium (19%), the grains are plump, flexible glue denseness (61 millimeters), Appearance Quality of Paddy Rice more excellent (chalky grain rate 15%, chalkiness degree
5%), low gelatinization point, cooking quality are excellent, between two parents；And Zhejiang spoke 111 be long-grained nonglutinous rice, amylose content 10%,
Grain is not full, and Rice Outer-quality is poor, and almost (chalkness ratio 90%, chalkiness degree is 40%) in vain for chalk；High quality japonica parent's show water 11
(16.3%) low in amylose content, flexible glue denseness (71 millimeters), low gelatinization point, the excellent (chalky grain rate of Appearance Quality of Paddy Rice
4%, chalkiness degree 0.2%), cooking quality it is excellent.
It should be appreciated that for those of ordinary skills, can be improved according to the above description or be converted,
And all these modifications and variations should all belong to the protection domain of claims of the present invention.
Claims (1)
1. a kind of high resistant starch content method for breeding nonglutinous rice, it is characterised in that carry out in accordance with the following steps：
(1) with high resistant starch content long-grained nonglutinous rice as parent, hybridize with low amylose content high quality japonica, hybridization obtains F1 generation kind
Son；
(2) F1 generation selfing, obtains F2 for seed；
(3) Jing rice type Oryza sativa L. is selected in colony in F2, the rice plant that setting percentage reaches more than 90% is harvested by individual plant,
First select rice plant of the chalkiness degree less than 10%, then analyze the Oryza sativa L. that the easy amylose content for determining is 17%～20% to plant
Strain, post analysis select the rice plant of high resistant starch content more than 8%；
(4) Jing rice type Oryza sativa L. is selected in strain in F3, the rice plant that setting percentage reaches more than 90% is harvested by individual plant,
Analysis selects chalkiness degree less than 10%, amylose content 17%～20% and the excellent list of high resistant starch content more than 8%
Strain；
(5) in F4 is for strain, by single-strain planting, the individual plant of high yield, mixed strain of receiving is selected to carry out rice physical and chemical quality detection, select
Caustic SCC is selected higher than 5 grades, the strain of more than 60 millimeters of flexible glue denseness；
(6), in F5～F7 self progenies, the step of repeat (5), carry out systematic breeding, low amylose content 15% in acquisition～
20%th, caustic SCC is higher than 5 grades, the high-quality high resistant starch content Jing rice of more than 60 millimeters of flexible glue denseness；
Wherein, high resistant starch content long-grained nonglutinous rice is Zhejiang spoke 111, and low amylose content high quality japonica parent is elegant water 11.
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| CN105165601B (en) * | 2015-09-29 | 2017-10-31 | 浙江大学 | A kind of selection of the special giant embryo rice varieties of rice with remained germ |
| CN105284594A (en) * | 2015-10-20 | 2016-02-03 | 浙江绿巨人生物技术有限公司 | Selection method of bi-functional rice variety |
| CN105830910B (en) * | 2016-04-02 | 2018-07-31 | 吉林省吉阳农业科学研究院 | A kind of selection of low glycemic index, high protein special type rice |
| CN109197566B (en) * | 2018-08-10 | 2021-07-13 | 浙江大学 | Breeding method of yellow endosperm rice |
| CN110447531A (en) * | 2019-09-18 | 2019-11-15 | 重庆市农业科学院 | A kind of selection of the high-resistance starch rice of diabetic |
| CN115152617B (en) * | 2022-07-07 | 2023-09-05 | 湖南农业大学 | Breeding method and application of high-resistance starch content and high-low temperature resistant indica rice variety |
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| CN103891596A (en) * | 2014-03-24 | 2014-07-02 | 浙江绿巨人生物技术有限公司 | Triple hybrid seed reproduction and production method for hybrid rice with high content of resistant starch |
| CN104094835A (en) * | 2013-04-12 | 2014-10-15 | 上海市农业科学院 | Breeding method of japonica rice variety capable of controlling postprandial blood sugar of diabetes patient |
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| CN104094835A (en) * | 2013-04-12 | 2014-10-15 | 上海市农业科学院 | Breeding method of japonica rice variety capable of controlling postprandial blood sugar of diabetes patient |
| CN103891596A (en) * | 2014-03-24 | 2014-07-02 | 浙江绿巨人生物技术有限公司 | Triple hybrid seed reproduction and production method for hybrid rice with high content of resistant starch |
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