CN107581059B - A kind of purple Chinese cabbage breeding method - Google Patents
A kind of purple Chinese cabbage breeding method Download PDFInfo
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- CN107581059B CN107581059B CN201711027077.8A CN201711027077A CN107581059B CN 107581059 B CN107581059 B CN 107581059B CN 201711027077 A CN201711027077 A CN 201711027077A CN 107581059 B CN107581059 B CN 107581059B
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Abstract
The invention belongs to breeding technical fields, disclose a kind of purple Chinese cabbage breeding method, with the excellent Chinese cabbage inbred lines P of Comprehensive Traits1For female parent, hybridize respectively with purple leaf mustard, purple cabbage leaves, purple tsai-tai and violet cabbage, obtains F1‑J、F1‑X、F1- C and F1- G, after planting from F1Chinese cabbage parent P is biased to for selection purple, Comprehensive Traits in plant1Plant, F1- J and F1- X hybridization obtains F2- JX, F1- C and F1- G hybridization obtains F2- CG, after planting F2For continuing to select in plant, F2- JX and F2- CG hybridization obtains F3For seed, after planting F3It is selected again in plant, with Chinese cabbage parent P1Backcrossing passes through mostly generation backcrossing, selection；It is selected by inbreeding of more generation, obtains whole strain purple Chinese cabbage breeding material.The present invention overcomes the deficiencies of the purple Chinese cabbage breeding material of traditional breeding way initiative only local purple.
Description
Technical field
The invention belongs to breeding technical field more particularly to a kind of purple Chinese cabbage breeding methods.
Background technique
The color of vegetables is its important feature, and an important factor for attraction consumer.Nutritionist thinks, vegetable nutrient
The height of content of material follows the rule of color from deep to shallow, and put in order total trend are as follows: black > purple > green
> red > yellow > white.And in same kind of vegetables, dark kind is more nutritious than light coloured variety.Purple vegetables are
Refer to that anthocyanidin content is high, purple or a kind of vegetables that are purplish red and having health-care efficacy are presented.Purple vegetable species are various, by edible
Position, which divides, can be divided into wild cabbage class, leaf vegetables, solanaceous vegetables, beans, tubers etc..Purple vegetables are increasingly consumed in recent years
The favor of person, edible purple food of arranging in pairs or groups have become trend.Purple vegetables possess strikingly color, full of nutrition, in dish color
In coloured silk collocation, have the function of that common vegetables with green leaves are incomparable, also has the function of centainly ornamental and high because of anthocyanidin content
Make it have certain health-care effect.A large number of studies show that anthocyanidin has very strong antioxidation, can remove intracorporal
Free radical reduces the activity of oxidizing ferment, improves the catabolism of high glycerine ester lipoprotein, inhibits cholesterol absorption, reduces low close
Spend lipoprotein cholesterol content；Resistance is different, antitumor, antiallergy, preventing hypertension and cardiovascular and cerebrovascular disease, slows down liver function energy barrier
Hinder, improve eyesight, adjusts mood etc..Therefore purple vegetables increasingly have been favored by people.Chinese cabbage [Brassica
Campestris L.ssp.pekinensis (Lour) Olsson] it is originating in China, it is full of nutrition, it is China's cultivated area and food
With the maximum vegetable crop of total amount, nearly 40,000,000 mu of annual planting area, the 13% of the total sown area of vegetables is accounted for；Sichuan Province in recent years
About 1,800,000 mu of Chinese cabbage annual planting area, account for about the 9% of vegetable cultivation area.If can be by purple gene transformation to common big
In Chinese cabbage, purple New Chinese Cabbage Variety is obtained, can not only enrich the diversity of Chinese cabbage cultivar, but also Chinese cabbage can be improved
Nutritive value and economic value.Therefore, the breeding work for carrying out purple Chinese cabbage has great importance.2006, Sun
Fly it is equal hybridized by Chinese cabbage with purple leaf mustard, continuous backcross, obtain the Chinese cabbage material of siphonal lobe purple；2008, Zhang De
Double equal F that purple is obtained using Chinese cabbage and purple cabbage leaves mutation intermolecular hybrid1Generation, then with Chinese cabbage mostly for backcross transformation,
Obtain the Chinese cabbage intermediate materials of drinamyl；2009, Zhang Lugang etc. was hybridized with Chinese cabbage with purple tsai-tai, and it is big to also obtain drinamyl
Chinese cabbage new germ plasm；2012, Qiao Haiyun etc. passed through the interspecific hybridization of Chinese cabbage and violet cabbage, obtained Chinese cabbage-violet cabbage kind
Between allodidiploid, siphonal lobe lavender, the vein and petiole of internal lobe are also purple, still, the allotriploid and great Bai
The BC that dish backcrossing obtains1It disappears for material Purpled traits.Up to the present, the report of domestic whole strain purple Chinese cabbage cultivar not yet
Road.
In conclusion problem of the existing technology is: the purple Chinese cabbage breeding material of traditional breeding way initiative is only
Local purple, the report of domestic whole strain purple Chinese cabbage cultivar not yet.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of purple Chinese cabbage breeding methods.
The invention is realized in this way a kind of purple Chinese cabbage breeding method, the purple Chinese cabbage breeding method include
Following steps:
Step 1, with the excellent Chinese cabbage inbred lines P of Comprehensive Traits1For female parent, respectively with purple leaf mustard PJ, purple little Bai
Dish PX, purple tsai-tai PCWith violet cabbage PGHybridization obtains F1-J、F1-X、F1- C and F1- G seed；
Step 2, after planting from F1Chinese cabbage parent P is biased to for selection purple, Comprehensive Traits in plant1Plant, F1-J
And F1- X hybridization obtains F2- JX, F1- C and F1- G hybridization obtains F2-CG；
Step 3, after planting from F2For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant,
F2- JX and F2- CG hybridization obtains F3For seed；
Step 4, after planting from F3For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant,
With Chinese cabbage parent P1Backcrossing obtains BC1Seed；
Step 5, after planting from BC1Continue to select purple, Comprehensive Traits close to Chinese cabbage parent P in plant1Plant, after
Continuous and Chinese cabbage parent P1Backcrossing, continuous multi-generation backcrossing, selection, obtains BCnSeed；
Step 6 can get character and stablize after planting using inbreeding of more generation homozygosis, color combining and economical character selection
Whole strain purple Chinese cabbage breeding material.
Further, mostly generation backcrossing is 3-6 generation backcrossing in the step 5.
Further, inbreeding of more generation is 2-4 generation selfing in the step 6.
Advantages of the present invention and good effect are as follows:, will using modern breedings means such as multiple aggregation hybridization, backcrossing and selfings
The Purpled traits transformation of a variety of Vegetables in Brassica selects the outstanding Chinese cabbage breeding material of whole strain purple into Chinese cabbage.
The present invention carries out base by modern breedings means such as multiple aggregation hybridization, backcrossing and selfings using existing germ plasm resource
Because of recombination, a kind of new method for hybridizing using a variety of Brassica genus purple vegetables and cultivating purple Chinese-cabbage Germplasm is created, is
The creation of purple Chinese-cabbage Germplasm provides a kind of new breeding methods.
The whole strain purple of purple Chinese cabbage breeding material that the present invention cultivates overcomes the purple of traditional breeding way initiative
The deficiency of Chinese cabbage breeding material only local purple.The present invention can be the further investigation of Chinese cabbage plant color genetic development,
New material base is provided.
Detailed description of the invention
Fig. 1 is purple Chinese cabbage breeding method flow chart provided in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Application principle of the invention is explained in detail with reference to the accompanying drawing.
As shown in Figure 1, purple Chinese cabbage breeding method provided in an embodiment of the present invention the following steps are included:
S101: with the excellent Chinese cabbage inbred lines (P of Comprehensive Traits1) it is maternal and purple leaf mustard (PJ) hybridization acquisition F1-J；
With the excellent Chinese cabbage inbred lines (P of Comprehensive Traits1) it is maternal and purple cabbage leaves (PX) hybridization acquisition F1-X；With Comprehensive Traits
Excellent Chinese cabbage inbred lines (P1) it is maternal and purple tsai-tai (PC) hybridization acquisition F1-C；Certainly with the excellent Chinese cabbage of Comprehensive Traits
Hand over system (P1) it is violet cabbage (PG) hybridization acquisition F1- G seed；
S102: after planting from F1Chinese cabbage parent P is biased to for selection purple, Comprehensive Traits in plant1Plant, F1- J and
F1- X hybridization obtains F2- JX, F1- C and F1- G hybridization obtains F2-CG；
S103: after planting from F2For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant, F2-
JX and F2- CG hybridization obtains F3For seed；
S104: after planting from F3For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant, with
Chinese cabbage parent P1Backcrossing obtains BC1Seed；
S105: after planting from BC1Continue to select purple, Comprehensive Traits close to Chinese cabbage parent P in plant1Plant, continue
With Chinese cabbage parent P1Backcrossing, continuous multi-generation backcrossing, selection, obtains BCnSeed；
S106: after planting using inbreeding of more generation homozygosis, color combining and economical character selection, it is stable to can get character
Whole strain purple Chinese cabbage breeding material.
The mostly generation backcrossing of step S105 is 3-6 generation backcrossing, and the inbreeding of more generation of step S106 is 2-4 generation selfing.
Application principle of the invention is further described below with reference to analysis.
The initiative of purple Chinese cabbage breeding material provided in an embodiment of the present invention includes turn of Juvenile stage, Purpled traits
It educates, be returned and be selfed.
Juvenile stage: the purple Chinese cabbage breeding material Comprehensive Traits in order to guarantee initiative are excellent, and female parent selects Chinese cabbage
Height is for self-mating system, and 95 days self-mating system breeding times, 59 centimetres of plant height, 76 centimetres of development degree, high yield, disease resistance is strong, soluble sugar
Content is high, and commodity property is good.
Male parent selects purple leaf mustard kind " red autumnal leaves leaf mustard " (P that Purpled traits are prominent, Comprehensive Traits are good respectivelyJ), purple
Varieties of Brassica campestris " purple hat " (PX), purple tsai-tai kind " two early son " (PC) and violet cabbage kind " early red " (PG), belong to cruciate flower
Section's Brassica genus is allied species or the mutation of Chinese cabbage.
The transformation of Purpled traits: selection is according to the super close convergent cross method for recombinating principle, and maternal Chinese cabbage is respectively and respectively
Paternal hybrid can retain the merit of maternal Chinese cabbage to greatest extent, reduce later period backcrossing and selfing algebra, effectively shorten and educate
The kind time limit, while also can be as much as possible by the Purpled traits transformation of each male parent into Chinese cabbage.
Chinese cabbage inbred lines DB41 (P1) and purple leaf mustard (PJ) hybridization acquisition F1- J seed DB41-J, Chinese cabbage inbred lines
DB41(P1) and purple cabbage leaves (PX) hybridization acquisition F1- X seed DB41-X, Chinese cabbage inbred lines DB41 (P1) and purple tsai-tai (PC)
Hybridization obtains F1- C seed DB41-C, Chinese cabbage inbred lines DB41 (P1) and violet cabbage (PG) hybridization acquisition F1- G seed DB41-G.
After planting from all F1Chinese cabbage parent P is biased to for selection purple, Comprehensive Traits in plant1Plant, with F1- J is
Female parent, F1- X is male parent, and hybridization obtains F2- JX seed DB41-J-X, separately with F1- C is female parent, F1- G is male parent, and hybridization obtains F2-
CG seed DB41-C-G.
After planting from F2For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant, with F2-JX
For female parent, F2- CG is male parent, and hybridization obtains F3For seed ZB41.
Backcrossing: after planting from F3For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant
ZB41-5 is female parent, with Chinese cabbage parent P1For male parent, backcrossing obtains BC1Seed.
After planting from BC1Continue to select purple, Comprehensive Traits close to Chinese cabbage parent P in plant1Plant ZB41-5-8 be
Female parent, with Chinese cabbage parent P1For male parent backcrossing, continuous selection and 4 generations of backcrossing, BC is obtained4Seed ZB41-5-8-1-4-2.
Selfing: being after planting selfed using 2 generations, keep the gene of Chinese cabbage breeding material sufficiently homozygous, in conjunction with color and
Economical character selection, the stable whole strain purple Chinese cabbage breeding material of acquired character.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (2)
1. a kind of purple Chinese cabbage breeding method, which is characterized in that the purple Chinese cabbage breeding method the following steps are included:
Step 1, with the excellent Chinese cabbage inbred lines P of Comprehensive Traits1For female parent, respectively with purple leaf mustard PJ, purple cabbage leaves PX、
Purple tsai-tai PCWith violet cabbage PGHybridization obtains F1-J、F1-X、F1- C and F1- G seed；
Step 2, after planting from F1Chinese cabbage parent P is biased to for selection purple, Comprehensive Traits in plant1Plant, F1- J and F1-X
Hybridization obtains F2- JX, F1- C and F1- G hybridization obtains F2-CG；
Step 3, after planting from F2For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant, F2-JX
And F2- CG hybridization obtains F3For seed；
Step 4, after planting from F3For continue in plant select purple, Comprehensive Traits be biased to Chinese cabbage parent P1Plant, and it is big
Chinese cabbage parent P1Backcrossing obtains BC1Seed；
Step 5, after planting from BC1Continue to select purple, Comprehensive Traits close to Chinese cabbage parent P in plant1Plant, continue with
Chinese cabbage parent P1Backcrossing, continuous multi-generation backcrossing, selection, obtains BCnSeed；
Step 6 can get stable whole of character after planting using inbreeding of more generation homozygosis, color combining and economical character selection
Strain purple Chinese cabbage breeding material；
Mostly generation backcrossing is 3-6 generation backcrossing in the step 5.
2. purple Chinese cabbage breeding method as described in claim 1, which is characterized in that inbreeding of more generation is 2- in the step 6
The selfing of 4 generations.
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| CN108207620B (en) * | 2018-02-13 | 2021-04-27 | 韶关学院 | High-anthocyanin-content mustard strain and breeding method thereof |
| CN109392697B (en) * | 2018-11-16 | 2021-09-17 | 东北农业大学 | Method for cultivating new purple Chinese cabbage germplasm by utilizing distant hybridization technology |
| CN110093346B (en) * | 2019-05-21 | 2019-12-27 | 中国农业科学院蔬菜花卉研究所 | Molecular marker linked with chromogene of brassica oleracea and application thereof |
| CN112056207A (en) * | 2020-09-29 | 2020-12-11 | 南京新创蔬菜分子育种研究院有限公司 | Breeding method of green stem black-bone vegetable material |
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| CN101416607A (en) * | 2008-12-08 | 2009-04-29 | 中国农业科学院蔬菜花卉研究所 | Claret Chinese cabbage breeding method |
| CN101480164A (en) * | 2009-02-18 | 2009-07-15 | 西北农林科技大学 | Breeding method of new germplasm of purple Chinese cabbage |
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| CN101416607A (en) * | 2008-12-08 | 2009-04-29 | 中国农业科学院蔬菜花卉研究所 | Claret Chinese cabbage breeding method |
| CN101480164A (en) * | 2009-02-18 | 2009-07-15 | 西北农林科技大学 | Breeding method of new germplasm of purple Chinese cabbage |
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Effective date of registration: 20210408 Address after: No.159, section 3, Huajin Avenue, Qingbaijiang District, Chengdu, Sichuan 610000 Patentee after: Institute of economic crop breeding and cultivation, Sichuan Academy of Agricultural Sciences Patentee after: Pengzhou Tianfu Shuxiang Agricultural Development Co.,Ltd. Address before: Agricultural science building, 159 Huajin Avenue, Qingbaijiang District, Chengdu, Sichuan 610300 Patentee before: Institute of economic crop breeding and cultivation, Sichuan Academy of Agricultural Sciences |
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Address after: No.159, section 3, Huajin Avenue, Qingbaijiang District, Chengdu, Sichuan 610000 Patentee after: INDUSTRIAL CROP Research Institute Patentee after: Pengzhou Rural Investment Development Co., Ltd Address before: No.159, section 3, Huajin Avenue, Qingbaijiang District, Chengdu, Sichuan 610000 Patentee before: INDUSTRIAL CROP Research Institute Patentee before: Pengzhou Tianfu vegetable Township Agricultural Development Co., Ltd |