CN101416607A - Claret Chinese cabbage breeding method - Google Patents
Claret Chinese cabbage breeding method Download PDFInfo
- Publication number
- CN101416607A CN101416607A CNA2008102394093A CN200810239409A CN101416607A CN 101416607 A CN101416607 A CN 101416607A CN A2008102394093 A CNA2008102394093 A CN A2008102394093A CN 200810239409 A CN200810239409 A CN 200810239409A CN 101416607 A CN101416607 A CN 101416607A
- Authority
- CN
- China
- Prior art keywords
- chinese cabbage
- aubergine
- plant
- male parent
- cabbage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the selection and cultivation method of purplish red cabbage. Plant generation F1 whose male parent is cabbage and female parent is purplish red rutabaga is cultivated by distant hybridization; the backcross of the hybrid plants takes cabbage as male parent; cabbage with stable hereditary performance and trait of purplish red is obtained by self-crossing purplish red plants whose general traits are similar to the traits of cabbage after generations of backcross. Different obvolvent types such as obvolvent , unrolling carpel, straight, overlapping-folded and conjunctionally folded can be obtained by the method according to the hereditary performance from male parent, meanwhile the growth season can be controlled in the same way. The invention enriches the breed variety of cabbage and leads a novel direction of breeding cabbage and improving the quality and nutritive value thereof.
Description
Technical field
The present invention relates to the brassicaceous vegetable breeding technical field, the selection of particularly a kind of aubergine Chinese cabbage.
Background technology
Chinese cabbage (Brassica rapa L.ssp.pekinensis) originates in China, be that the Cruciferae rape belongs to annual, biennial herbaceous plant, comprise that balling reaches not balling two big monoids, be one of most important vegetable crop during rape belongs to, be still so far China distribute the widest, area under cultivation is maximum, the edible maximum bulk vegetable of crowd.
Along with improving constantly of people's living standard, the Chinese cabbage breeding for quality is subjected to numerous breeders' attention day by day.In recent years, orange heart Chinese cabbage, extremely consumer's the favor of breeding in succession both at home and abroad of gold zone Chinese cabbage.The new direction of new, strange, the especially big Chinese cabbage breeding of new variety of different ball look breedings having become is one of research contents of breeding for quality.
Rape belongs in the vegetables at present purple cabbage, purple tsai-tai, purple leaf mustard etc., does not but see purple Chinese cabbage resource.If purple can be changed over to common Chinese cabbage, obtain purple Chinese cabbage new varieties, not only can enrich Chinese cabbage cultivar, and, produce new material high-quality, high nutritive value and have great importance for the Chinese cabbage breeding for quality.
Summary of the invention
The selection that the purpose of this invention is to provide a kind of aubergine Chinese cabbage.
The present invention selects the stable leaf mustard material of natural aubergine hereditary capacity for maternal, the comprehensive good Chinese cabbage of proterties is a male parent, save and continuous backcross selfing again by distant hybridization, embryo, obtain aubergine Chinese cabbage material, this method can obtain the stable aubergine Chinese cabbage of hereditary capacity.
Wherein, described aubergine leaf mustard material is PM058; Described Chinese cabbage is a Chinese cabbage DH system.
That is, the technical solution used in the present invention is:
Is that distant hybridization obtains aubergine F1 plant by aubergine leaf mustard PM058 for maternal and Chinese cabbage DH, be that male parent is backcrossed with Chinese cabbage DH again, identify that in conjunction with the field selecting the aubergine plant is continuous backcross with Chinese cabbage DH, obtain mauve Chinese cabbage backcross plant, obtain the stable aubergine Chinese cabbage material of hereditary capacity by selfing again, specifically comprise:
1) selection obtains the aubergine leaf mustard material PM058 of inheritance stability;
2) select to obtain the good Chinese cabbage DH system of comprehensive proterties;
3) be maternal with PM058, the Chinese cabbage DH that proterties is good is the male parent distant hybridization, obtains the F1 plant by embryo rescue techniques;
4) do with aubergine F1 plant maternal, with above-mentioned Chinese cabbage DH be that male parent is backcrossed and obtained the BC1 seed;
5) carry out the field with the BC1 seed and identify, it is maternal to select the aubergine plant to do, with above-mentioned Chinese cabbage DH be that male parent continues to backcross, select by the field repeatedly and continuous backcross obtains to bloom normally, the normal aubergine BCn plant of setting seeds;
6) aubergine BCn plant is carried out selfing and obtain seed BCnS1;
Method of the present invention also comprises:
7) BCnS1 is identified through the field and in conjunction with selecting, obtain inheritance stability, the plant color shows as and the maternal identical mauve Chinese cabbage plant of leaf mustard.
8) the mauve Chinese cabbage plant that step 7) is obtained continues selfing and expands a large amount of mauve Chinese cabbage seeds of numerous acquisition.
Wherein, PM058 is that the purple leaf mustard landrace red autumnal leaves green vegetables of selecting Qianyang, Hunan (draw from country of Vegetable ﹠ Flower Inst., Chinese Academy of Agriculture Science variety source and preserve the storehouse mid-term, the number of introducing a fine variety: II 3A0138), by preferably involving continuous individual plant selfing in a criminal case, the aubergine leaf mustard of the inheritance stability of the acquisition inbred line of isozygotying;
Chinese cabbage DH system cultivates the Chinese cabbage seeds commonly used of market purchasing by the Isolated microspore technology, isozygotying that the proterties of acquisition is stable is.
Described isolated microspore culture technique is emerging in recent years culture technique, but list of references: Sato et al., 1989, Plant regeneration from isolated microspore culture ofChinese cabbage (Brassica campestris spp.pekinensis) .Plant CellReports, 8:486-488; Hang F.L., Takahata y., 2001, Inheritance ofmicrospore embryogenic ability in Brassica crops., Theor.Appl.Genet., 103:254～258, concrete steps comprise:
I) choosing length is 2～3mm, and microspore mainly is in the keep to the side Chinese cabbage bud of phase of monokaryon, and with 75% alcohol-pickled 30s, 7% liquor natrii hypochloritis washs 15min, sterile distilled water flushing 3 times;
Ii) extract medium and extract microspore with B5, after the aseptic micropore filtered through gauze of 30 μ m centrifugal 3 times, each 1000rmin
-1, 5min, free microspore is used NLN-13 (sucrose concentration 13%) medium culture, and it is 1～2 * 10 that blood cell counting plate is adjusted microspore concentration
5ML
-1, behind 33 ℃ of heat-inducible 24h, change 25 ℃ of dark cultivations over to and form until adventitious embryo;
Iii) when embryonic development is cotyledon period, change B5 solid culture medium successive transfer culture over to, until obtaining the DH normal plant that nature doubles;
Iv) will organize the domestication of emerging of microspore DH plant that training obtains, and obtain seed by pollination self, in the field evaluation, it is standby selecting the good DH of comprehensive proterties.
Since with the Isolated microspore technology cultivate the Chinese cabbage plant to add multiplying power naturally very high, utilize this technology can obtain the Chinese cabbage DH that isozygotys easily to be.
Described B5 extracts medium according to document: Gamborg, O.L., et al., 1968, Nutrientrequirements of suspension cultures of soybean root cells.Exp.Cell Res.50, the B5 medium described in the 151-158. adds sucrose 130.0gL
-1, adjusting the pH value of solution value then is that 5.8-6.0 makes.Autoclaving (121 ℃, 15min) after, cool off standby.
Described NLN-13 medium according to document: J.B.M.Custers.2003.Microsporeculture in rapeseed (Brassica napus L.) //M.Maluszynski etal. (eds.), Doubled Haploid Production in Crop Plants.Published byKluwer Academic Publishers, composition described in the Printed in the Netherlands:185-193., adjusting the pH value of solution value then is 6.0,0.22 μ m pin type filter filtration sterilization makes, 4 ℃ of preservations are standby.
The aubergine plant normal, that set seeds normal that be to obtain to bloom, step 5) need to select by the field repeatedly and with Chinese cabbage DH be the male parent continuous backcross, the number of occurrence is generally 3-6 time.
Described embryo rescue techniques is: carry out artificial bud pollination for maternal and male parent, the male parent fresh pollen be applied in female parent (detassel) bud column cap, early, evening respectively once, continuous three days.
Got the pollination back 7-12 days, the angle fruit that ovary has been swelled, with 75% alcohol-pickled 30s, 7% liquor natrii hypochloritis washs 15min on superclean bench, sterile distilled water flushing 3 times.
Carrying out ovary with the B5 solid culture medium cultivates.Condition of culture is 25 ℃ of culturing room, intensity of illumination 1000-1500lx, illumination every day 14h.
The ovary of cultivating 10-15 days is peeled off green embryo with the dissecting needle or the tweezers of sterilization on superclean bench, continue to be put in the B5 solid culture medium and cultivate.Condition of culture is cultivated with ovary, until the normal F1 tissue cultivating seedling plant that obtains tool root, stem, leaf.
Described B5 solid culture medium is: at former B5 solid culture medium (Gamborg, O.L., et al., 1968, Nutrient requirements of suspension cultures of soybean root cells.Exp.Cell Res.50,151-158.) the following material of middle adding, making its concentration is 6-benzyl aminopurine (6-BA) 1.5mgL
-1, α-Nai Yisuan (NAA) 0.05mgL
-1, sucrose 30.0gL
-1, agar 7.0gL
-1, adjusting the pH value of solution value then is 5.8-6.0, is sub-packed in the 100ml triangular flask.Autoclaving (121 ℃, 15min) after, it is standby to be put in the testing stand cooled and solidified.
According to the selected Chinese cabbage genotype shape of male parent of backcrossing, by above-mentioned selection, can obtain different obvolvent types: comfortable, straight tube, foldedly embrace, fill the span of a man's arms etc., and the aubergine Chinese cabbage material of different growing, greatly enriched the kind of Chinese cabbage, be the breeding for quality of Chinese cabbage, produce new material high-quality, high nutritive value direction is provided.
Description of drawings
Fig. 1 is the breeding line spectrogram of aubergine Chinese cabbage.
Fig. 2 for male parent be in the comfortable aubergine Chinese cabbage of white 78C1.
Fig. 3 is the folded armful aubergine Chinese cabbage of orange heart C24 for male parent.
Fig. 4 is the precocious aubergine Chinese cabbage of Xiayang 50C1 for male parent.
Fig. 5 for male parent be in the late-maturing aubergine Chinese cabbage of white 78C16.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
Select the purple leaf mustard landrace red autumnal leaves green vegetables in Qianyang, Hunan (to draw from country of Vegetable ﹠ Flower Inst., Chinese Academy of Agriculture Science variety source and preserve the storehouse mid-term, the number of introducing a fine variety: II 3A0138), by preferably involving continuous individual plant selfing in a criminal case, selfing 3-5 generation, the selection offspring does not have the proterties separation, cauline leaf all is mauve leaf mustard selfed seed, thereby obtains the inbred line PM058 that isozygotys of inheritance stability.
Preparation B5 extracts medium: according to document Gamborg, O.L., et al., 1968, Nutrientrequirements of suspension cultures of soybean root cells.Exp.Cell Res.50,151-158. described in B5 medium, prepare macroelement (concentrating 20 times) earlier, trace element (concentrating 200 times), molysite (concentrating 200 times) and the organic substance except that sucrose (concentrating 200 times) concentrate stock solution, get the 1L volumetric flask then, get macroelement and concentrate stock solution 50ml, trace element, molysite and above-mentioned organic substance concentrate each 5ml of stock solution, and it is 130.0gL that interpolation sucrose makes its concentration
-1, the distilled water constant volume, then, adjusting the pH value of solution value is that 5.8-6.0 makes.Autoclaving (121 ℃, 15min) after, cool off standby.
Preparation NLN-13 medium: complete reference literature J.B.M.Custers.2003.Microspore culture in rapeseed (Brassica napus L.) //M.Maluszynskiet al. (eds.), Doubled Haploid Production in Crop Plants.Published byKluwer Academic Publishers, NLN-13 culture medium preparation method described in the Printed in the Netherlands:185-193., adjusting the pH value of solution value then is 6.0,0.22 μ m pin type filter filtration sterilization makes, 4 ℃ of preservations are standby.
Select Chinese cabbage cultivar " in white 78 " (available from vegetables gardening breeding research and development centre in Vegetable ﹠ Flower Inst., Chinese Academy of Agriculture Science Beijing), cultivate the DH system of isozygotying by the Isolated microspore technology, step is as follows:
I) choosing length is 2～3mm, and microspore mainly is in the keep to the side Chinese cabbage bud of phase of monokaryon, and with 75% alcohol-pickled 30s, 7% liquor natrii hypochloritis washs 15min, sterile distilled water flushing 3 times;
Ii) extract medium and extract microspore with B5, after the aseptic micropore filtered through gauze of 30 μ m centrifugal 3 times, each 1000rmin
-1, 5min, free microspore is used NLN-13 (sucrose concentration 13%) medium culture, and it is 1～2 * 10 that blood cell counting plate is adjusted microspore concentration
5.mL
-1, change 25 ℃ of dark cultivations behind 33 ℃ of heat-inducible 24h over to, form until adventitious embryo;
Iii) change B5 solid culture medium successive transfer culture during for cotyledon period over to when embryonic development, obtain the DH normal plant that nature doubles;
Iv) will organize the domestication of emerging of microspore DH plant that training obtains, and obtain seed by pollination self and identify in the field, and select " in white 78C1 " and late-maturing " in 78C16 " in vain of comfortable proterties, the DH that other proterties are good is standby.
Adopt above-mentioned isolated microspore culture technique, select Chinese cabbage cultivar " orange No. two of Beijing " (available from Vegetable Research Centre, Beijing Academy of Agriculture and Forest Sciences) and " Xiayang 50 days " (commercial), cultivate respectively, select folded " orange heart C24 " and the precocious Chinese cabbage " Xiayang 50C1 " that embraces proterties, the DH that other proterties are good is standby.
Embodiment 2
Configuration B5 solid culture medium: according to document Gamborg, O.L., et al., 1968, Nutrientrequirements of suspension cultures of soybean root cells.Exp.Cell Res, 50,151-158. described in B5 medium, prepare macroelement (concentrating 20 times) earlier, trace element (concentrating 200 times), molysite (concentrating 200 times) and the organic substance except that sucrose (concentrating 200 times) concentrate stock solution, get the 1L volumetric flask then, get macroelement and concentrate stock solution 50ml, trace element, molysite and above-mentioned organic substance concentrate each 5ml of stock solution, add following material in addition, making its concentration is 6-benzyl aminopurine (6-BA) 1.5mgL
-1, α-Nai Yisuan (NAA) 0.05mgL
-1, sucrose 30.0gL
-1, agar 7.0gL
-1, the distilled water constant volume, then, adjusting the pH value of solution value is 5.8-6.0, is sub-packed in the 100ml triangular flask.Autoclaving (121 ℃, 15min) after, cooled and solidified is standby.
With aubergine leaf mustard PM058 is maternal, and white 78C1 is the male parent distant hybridization in the Chinese cabbage DH system, carries out artificial bud pollination for maternal and male parent, and the male parent fresh pollen is applied in female parent (detassel) bud column cap, early, evening respectively once, continuous three days.
Get back 10 days of pollination, the angle fruit that ovary has been swelled, with 75% alcohol-pickled 30s, 7% liquor natrii hypochloritis washs 15min on superclean bench, sterile distilled water flushing 3 times.
Carrying out ovary with the B5 solid culture medium cultivates.Condition of culture is 25 ℃ of culturing room, intensity of illumination 1000-1500lx, illumination every day 14h.
The ovary of cultivating 15 days is peeled off green embryo with the dissecting needle or the tweezers of sterilization on superclean bench, continue to be put in the B5 solid culture medium and cultivate.Condition of culture is cultivated with ovary, to obtain the normal F1 tissue cultivating seedling plant of tool root, stem, leaf.
Do maternally with the aubergine F1 plant that obtains, white 78C1 is male parent acquisitions (in 78C1) the in vain BC1 seed of backcrossing in the Chinese cabbage DH system;
(in white 78C1) the BC1 seed that obtains is carried out the field identify, it is maternal to select the aubergine plant to do, and is male parent acquisitions (in 78C1) the in vain BC2 seed that continues to backcross with white 78C1 in the Chinese cabbage DH system;
(in white 78C1) the BC2 seed that obtains is carried out the field to be identified, it is maternal to select the aubergine plant to do, with white 78C1 in the Chinese cabbage DH system is that male parent continues to backcross, by the field select and continuous backcross obtain to bloom normal, the normal aubergine of setting seeds (in 78C1) in vain BC5 plant;
The normal aubergine of blossoming and having seeds that obtains (in white 78C1) BC5 plant is carried out selfing obtain seed (in white 78C1) BC5S1;
(in white 78C1) the BC5S1 selfed seed that obtains is carried out the field to be identified, it is identical with the maternal PM058 of leaf mustard that selection obtains that hereditary capacity is stable, the plant color shows as, and the approaching aubergine Chinese cabbage plant (see figure 2) of 78C1 in vain in other proterties and the Chinese cabbage DH system.
(in white 78C1) BC5S1 aubergine Chinese cabbage plant selfing that evaluation is obtained is expanded numerous, obtains a large amount of seeds of the stable aubergine Chinese cabbage of hereditary capacity (in white 78C1) BC5S1.
Embodiment 3
With aubergine leaf mustard PM058 is female parent, and Chinese cabbage DH is that orange heart C24 is the male parent distant hybridization, obtains the F1 plant by embryo rescue techniques;
Do maternally with the aubergine F1 plant that obtains, Chinese cabbage DH is that orange heart C24 is male parent acquisition (orange heart C24) the BC1 seed of backcrossing;
(orange heart C24) the BC1 seed that obtains is carried out the field identify, it is maternal to select the aubergine plant to do, and is that orange heart C24 is male parent acquisition (orange heart C24) the BC2 seed that continues to backcross with Chinese cabbage DH;
(orange heart C24) the BC2 seed that obtains is carried out the field to be identified, it is maternal to select the aubergine plant to do, with Chinese cabbage DH is that orange heart C24 is that male parent continues to backcross, and it is normal to obtain to bloom by field selection and continuous backcross, the normal aubergine of setting seeds (orange heart C24) BC6 plant;
The normal aubergine of blossoming and having seeds (orange heart C24) the BC5 plant that obtains is carried out selfing obtain seed (orange heart C24) BC6S1;
(orange heart C24) the BC6S1 selfed seed that obtains is carried out the field to be identified, it is identical with the maternal PM058 of leaf mustard that selection obtains that hereditary capacity is stable, the plant color shows as, and other proterties and the Chinese cabbage DH approaching aubergine Chinese cabbage plant (see figure 3) that is orange heart C24.
(orange heart C24) BC6S1 aubergine Chinese cabbage plant selfing expansion that evaluation obtains is numerous, a large amount of seeds of aubergine Chinese cabbage (the orange heart C24) BC6S1 that the acquisition hereditary capacity is stable.
Embodiment 4
With aubergine leaf mustard PM058 is female parent, and Chinese cabbage DH is that Xiayang 50C1 is the male parent distant hybridization, obtains the F1 plant by embryo rescue techniques;
Do maternally with the aubergine F1 plant that obtains, Chinese cabbage DH is that Xiayang 50C1 is male parent acquisition (Xiayang 50C1) the BC1 seed of backcrossing;
(Xiayang 50C1) the BC1 seed that obtains is carried out the field identify, it is maternal to select the aubergine plant to do, and is that Xiayang 50C1 is male parent acquisition (Xiayang 50C1) the BC2 seed that continues to backcross with Chinese cabbage DH;
(Xiayang 50C1) the BC2 seed that obtains is carried out the field to be identified, it is maternal to select the aubergine plant to do, with Chinese cabbage DH is that Xiayang 50C1 is that male parent continues to backcross, and it is normal to obtain to bloom by field selection and continuous backcross, the normal aubergine of setting seeds (Xiayang 50C1) BC5 plant;
The normal aubergine of blossoming and having seeds (Xiayang 50C1) the BC5 plant that obtains is carried out selfing obtain seed (Xiayang 50C1) BC5S1;
(Xiayang 50C1) the BC5S1 selfed seed that obtains is carried out the field to be identified, it is identical with the maternal PM058 of leaf mustard that selection obtains that hereditary capacity is stable, the plant color shows as, and other proterties and the Chinese cabbage DH approaching aubergine Chinese cabbage plant (see figure 4) that is Xiayang 50C1.
(Xiayang 50C1) BC5S1 aubergine Chinese cabbage plant selfing expansion that evaluation obtains is numerous, a large amount of seeds of aubergine Chinese cabbage (Xiayang 50C1) BC5S1 that the acquisition hereditary capacity is stable.
Embodiment 5
With aubergine leaf mustard PM058 is maternal, and white 78C16 is the male parent distant hybridization in the Chinese cabbage DH system, carries out artificial bud pollination for maternal and male parent, and the male parent fresh pollen is applied in female parent (detassel) bud column cap, early, evening respectively once, continuous three days.
Get back 10 days of pollination, get the angle fruit that ovary has been swelled, with 75% alcohol-pickled 30s, 7% liquor natrii hypochloritis washs 15min on superclean bench, sterile distilled water flushing 3 times.
Carrying out ovary with the B5 solid culture medium cultivates.Condition of culture is 25 ℃ of culturing room, intensity of illumination 1000-1500lx, illumination every day 14h.
The ovary of cultivating 15 days is peeled off green embryo with the dissecting needle or the tweezers of sterilization on superclean bench, continue to be put in the B5 solid culture medium and cultivate.Condition of culture is cultivated with ovary, to obtain the normal F1 tissue cultivating seedling plant of tool root, stem, leaf.
Do maternally with the aubergine F1 plant that obtains, white 78C16 is male parent acquisitions (in 78C16) the in vain BC1 seed of backcrossing in the Chinese cabbage DH system;
(in white 78C16) the BC1 seed that obtains is carried out the field identify, it is maternal to select the aubergine plant to do, and is male parent acquisitions (in 78C16) the in vain BC2 seed that continues to backcross with white 78C16 in the Chinese cabbage DH system;
(in white 78C16) the BC2 seed that obtains is carried out the field to be identified, it is maternal to select the aubergine plant to do, with white 78C16 in the Chinese cabbage DH system is that male parent continues to backcross, by the field select and continuous backcross obtain to bloom normal, the normal aubergine of setting seeds (in 78C16) in vain BC5 plant;
The normal aubergine of blossoming and having seeds that obtains (in white 78C16) BC5 plant is carried out selfing obtain seed (in white 78C16) BC5S1;
(in white 78C16) the BC5S1 selfed seed that obtains is carried out the field to be identified, it is identical with the maternal PM058 of leaf mustard that selection obtains that hereditary capacity is stable, the plant color shows as, and the approaching aubergine Chinese cabbage plant (see figure 5) of 78C16 in vain in other proterties and the Chinese cabbage DH system.
(in white 78C16) BC5S1 aubergine Chinese cabbage plant selfing that evaluation is obtained is expanded numerous, obtains a large amount of seeds of the stable aubergine Chinese cabbage of hereditary capacity (in white 78C16) BC5S1.
Claims (7)
1, the selection of a kind of aubergine Chinese cabbage, it is characterized in that the aubergine leaf mustard for maternal, Chinese cabbage obtains aubergine F1 plant for the male parent distant hybridization, backcross with the Chinese cabbage male parent again, select the aubergine plant to use Chinese cabbage male parent continuous backcross in conjunction with the field evaluation, obtain mauve Chinese cabbage backcross plant, obtain the stable aubergine Chinese cabbage material of hereditary capacity by selfing again.
2, the method for claim 1 is characterized in that, described aubergine leaf mustard is PM058.
3, the method for claim 1 is characterized in that, described Chinese cabbage is a Chinese cabbage DH system.
4, as claim 2 or 3 described methods, it is characterized in that, comprising:
1) selection obtains the aubergine leaf mustard material PM058 of inheritance stability;
2) select to obtain the good Chinese cabbage DH system of comprehensive proterties;
3) be maternal with PM058, the Chinese cabbage DH that proterties is good is the male parent distant hybridization, obtains the F1 plant by embryo rescue techniques;
4) do with aubergine F1 plant maternal, with above-mentioned Chinese cabbage DH be that male parent is backcrossed and obtained the BC1 seed;
5) carry out the field with the BC1 seed and identify, it is maternal to select the aubergine plant to do, with above-mentioned Chinese cabbage DH be that male parent continues to backcross, select by the field repeatedly and continuous backcross obtains to bloom normally, the normal aubergine BCn plant of setting seeds;
6) aubergine BCn plant is carried out selfing and obtain seed BCnS1.
5, method as claimed in claim 4 also comprises:
1) BCnS1 that step 5) is obtained also in conjunction with selection, obtains inheritance stability through the field evaluation, the plant color shows as and the identical mauve Chinese cabbage plant of leaf mustard female parent;
2) the mauve Chinese cabbage seeds of numerous acquisition is expanded in the mauve Chinese cabbage plant continuation selfing that will go up the step acquisition.
6, method as claimed in claim 4 is characterized in that, the number of times of backcrossing of step 5) is 3-6 time.
7, method as claimed in claim 4 is characterized in that step 2) utilize isolated microspore culture technique to carry out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102394093A CN101416607B (en) | 2008-12-08 | 2008-12-08 | Claret Chinese cabbage breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102394093A CN101416607B (en) | 2008-12-08 | 2008-12-08 | Claret Chinese cabbage breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101416607A true CN101416607A (en) | 2009-04-29 |
| CN101416607B CN101416607B (en) | 2011-04-27 |
Family
ID=40627758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102394093A Expired - Fee Related CN101416607B (en) | 2008-12-08 | 2008-12-08 | Claret Chinese cabbage breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101416607B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919117A (en) * | 2012-11-14 | 2013-02-13 | 中国农业科学院蔬菜花卉研究所 | Method for quickly transforming cytoplasmic male sterile line of brassica vegetables |
| CN101627724B (en) * | 2009-08-06 | 2013-09-04 | 广州紫罗兰园林科技有限公司 | Cultivation and acclimatization technology of new gillyflower tree hybridization species |
| CN103461100A (en) * | 2013-08-26 | 2013-12-25 | 安徽省农业科学院园艺研究所 | Purple Wuta-tsai breeding method |
| CN104429944A (en) * | 2014-11-04 | 2015-03-25 | 中国热带农业科学院椰子研究所 | Method for preparing sterile oil palm seed embryo sample in batch |
| CN107581059A (en) * | 2017-10-27 | 2018-01-16 | 四川省农业科学院经济作物育种栽培研究所 | A kind of purple Chinese cabbage breeding method |
| CN109392697A (en) * | 2018-11-16 | 2019-03-01 | 东北农业大学 | A method of purple Chinese cabbage new germ plasm is cultivated using distant hybridization technology |
| CN113999931A (en) * | 2021-09-03 | 2022-02-01 | 泰安市农业科学研究院 | Method for auxiliary breeding by using molecular markers related to Chinese cabbage flower colors |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887061A (en) * | 2006-06-15 | 2007-01-03 | 西北农林科技大学 | Breeding method of Chinese cabbage hybrid |
-
2008
- 2008-12-08 CN CN2008102394093A patent/CN101416607B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627724B (en) * | 2009-08-06 | 2013-09-04 | 广州紫罗兰园林科技有限公司 | Cultivation and acclimatization technology of new gillyflower tree hybridization species |
| CN102919117A (en) * | 2012-11-14 | 2013-02-13 | 中国农业科学院蔬菜花卉研究所 | Method for quickly transforming cytoplasmic male sterile line of brassica vegetables |
| CN102919117B (en) * | 2012-11-14 | 2014-07-16 | 中国农业科学院蔬菜花卉研究所 | Method for quickly transforming cytoplasmic male sterile line of brassica vegetables |
| CN103461100A (en) * | 2013-08-26 | 2013-12-25 | 安徽省农业科学院园艺研究所 | Purple Wuta-tsai breeding method |
| CN103461100B (en) * | 2013-08-26 | 2015-11-18 | 安徽省农业科学院园艺研究所 | A kind of selection of purple Wuta-tsai |
| CN104429944A (en) * | 2014-11-04 | 2015-03-25 | 中国热带农业科学院椰子研究所 | Method for preparing sterile oil palm seed embryo sample in batch |
| CN107581059A (en) * | 2017-10-27 | 2018-01-16 | 四川省农业科学院经济作物育种栽培研究所 | A kind of purple Chinese cabbage breeding method |
| CN107581059B (en) * | 2017-10-27 | 2019-11-05 | 四川省农业科学院经济作物育种栽培研究所 | A kind of purple Chinese cabbage breeding method |
| CN109392697A (en) * | 2018-11-16 | 2019-03-01 | 东北农业大学 | A method of purple Chinese cabbage new germ plasm is cultivated using distant hybridization technology |
| CN109392697B (en) * | 2018-11-16 | 2021-09-17 | 东北农业大学 | Method for cultivating new purple Chinese cabbage germplasm by utilizing distant hybridization technology |
| CN113999931A (en) * | 2021-09-03 | 2022-02-01 | 泰安市农业科学研究院 | Method for auxiliary breeding by using molecular markers related to Chinese cabbage flower colors |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101416607B (en) | 2011-04-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101416607B (en) | Claret Chinese cabbage breeding method | |
| KR102198083B1 (en) | Selection and Breeding of Cruciferous Vegetable Ingredients and Varieties in Rapeseed Double Haploid Induction System | |
| CN103975849B (en) | A kind of precocity, the breeding of disease-resistant, the pale reddish brown Broccoli of loose type and breeding method | |
| CN101803564B (en) | Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines | |
| CN103444552A (en) | Method for inducing eggplant anther to regenerate haplobiont | |
| CN102919117A (en) | Method for quickly transforming cytoplasmic male sterile line of brassica vegetables | |
| CN102210259B (en) | Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization | |
| CN111109072A (en) | Breeding method of purple-yellow leaf ornamental type rape line | |
| CN103348910B (en) | Method for transforming sterile line of Chinese kale by utilizing vernalization and generation adding | |
| CN103299896A (en) | Culturing method of eurytropic bolting-resisting spring Chinese cabbage free microspores | |
| CN103975848B (en) | Breeding and cultivating method for early-matured and disease-resistant brassica oleracea | |
| CN102144560B (en) | Method and application method for obtaining novel germ plasm of brassica A genome vegetable | |
| CN103548672B (en) | The seed selection of cabbage heart cytoplasmic male sterile line, breeding and producing method for seed | |
| Wang et al. | Initiation and development of microspore embryogenesis in recalcitrant purple flowering stalk (Brassica campestris ssp. chinensis var. purpurea Hort.) genotypes | |
| CN103168681B (en) | Breeding method of early-ripe and disease-resistant broccolini | |
| CN111789025B (en) | Breeding method of ornamental special rape variety | |
| CN101878734B (en) | Method for constructing collard cytoplasmic male sterility near-isogenic lines | |
| CN103988775A (en) | Method for breeding and cultivating early ripening disease-resistant stem broccoli | |
| CN108633660B (en) | A kind of Radix Salviae Miltiorrhizae new varieties production of hybrid seeds method for culturing seedlings | |
| CN103190336A (en) | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids | |
| CN110100721A (en) | A kind of purplish red leaf sightseeing type multipurpose cabbage type rape selection | |
| CN101228841B (en) | Method of using anther culture rapidly selecting wheat photo-thermoperiod sensitive genic male sterile lines | |
| CN115380815B (en) | Breeding method of male sterile line of cabbage mustard | |
| CN103798125A (en) | Method for acquiring novel species of brassicaceous vegetables and application method of novel specie of brassicaceous vegetables | |
| CN110073972B (en) | Breeding method of purple-leaf white-flower cabbage type hybrid rape combination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110427 Termination date: 20201208 |