CN103461100B - A kind of selection of purple Wuta-tsai - Google Patents
A kind of selection of purple Wuta-tsai Download PDFInfo
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- CN103461100B CN103461100B CN201310376427.7A CN201310376427A CN103461100B CN 103461100 B CN103461100 B CN 103461100B CN 201310376427 A CN201310376427 A CN 201310376427A CN 103461100 B CN103461100 B CN 103461100B
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Abstract
The invention discloses a kind of selection of purple Wuta-tsai, the method is with aubergine Chinese cabbage PC601 for female parent, shows as mauve F1 generation plant by hybridizing acquisition with Wuta-tsai inbred line YW905; With inbred line YW905 for the male parent that backcrosses backcrosses to hybrid plant; Through too much generation backcrosses, select Comprehensive Traits to be that aubergine plant carries out selfing close to YW905 proterties, plant color, obtain inheritance stability, variable rate technology is mauve Wuta-tsai material.Selection of the present invention, according to the genotype shape of selected Wuta-tsai male parent, the aubergine Wuta-tsai material of Different ripening stages, Different plant type can be obtained, greatly enriched the kind of Wuta-tsai, for the breeding for quality of Wuta-tsai, the new material of production high-quality, high nutritive value provides direction.
Description
Technical field
The present invention relates to crossbreeding technology field, in particular a kind of selection of aubergine Wuta-tsai.
Background technology
The color and luster of vegetable crop is the key character of a product, is also the importance attracting consumer.Colorful vegetable compared with common vegetable, often have bright in luster, nutritious, cultivation benefit is high and can be used for the features such as rural area sightseeing.From nutrition science angle, from deep to shallow, also from high to low, put in order total is black, purple, green, redness, yellow, white to its nutritive value to vegetable colour.In the vegetables of one species, dark kind is more nutritious than light coloured variety, and this is also one of purple vegetables reason in one's favour.It will be further appreciated that the anthocyan in purple vegetables, it is except possessing very strong oxidation resistance, preventing hypertension, slowing down except the effect of hepatosis lamp, and it improves the effect such as eyesight, prevention eye strain and is also admitted by a lot of people.For women, anthocyan is good assistant anti-aging in advance, its good oxidation resistance, can help to regulate free radical, and the crowd of Long-Time Service computer or reading more should absorb more.
Wuta-tsai originates from basin, China middle and lower reach of Yangtze River, now the cultivation history of existing nearly one thousand years.Crow dish adaptability is comparatively wide, and current national most area all has cultivation, and some places are as special vegetable introduction of plant.Wherein south cultivation is more, especially in Anhui, Shanghai, the ground food custom such as Jiangsu is very general, area under cultivation is larger.One of the famous special product in Wu Caishi Anhui Province, Anhui, there is the advantages such as distribution is wide, output is high, raciness, supply phase length, can whole year production, the four seasons supply, especially planted in fall is applicable to, with sweet and famous through frost and snow aftertaste, be regarded as the treasure in Chinese cabbage, be the desirable vegetables of adjustment " winter lacks " and " spring is light ", just cultivate as important vegetables since ancient times.
In recent years, along with the development of productivity and the raising of living standards of the people, breeding for quality is subject to the attention of Chinese scholars day by day.Color breeding has become the new direction of quality of vegetable breeding.In order to meet the demand in market, successfully select the kinds such as violet cabbage, purple tsai-tai, Exocarpium Citri Rubrum heart Chinese cabbage in brassicaceous vegetable, and the parent obtaining consumer looks at.But report has been had not yet to see for the Wuta-tsai kind of purple.In order to enrich Wuta-tsai resource, increase the diversity of Wuta-tsai kind, and meet the requirement of people to Wuta-tsai quality and specialty goods further, cultivate the fresh target that purple Wuta-tsai kind becomes Wuta-tsai breeding.
Summary of the invention
Technical problem to be solved of the present invention is for the deficiencies in the prior art, utilizes hybridization, continuous backcross selfing technology again, provides a kind of purple Wuta-tsai selection, obtains purple Wuta-tsai material.
In the practices of breeding, purple gene is dominant inheritance to green, and namely purple leaf plant and greenery plant filial generation blade show as purple, therefore with aubergine Chinese cabbage and common Wuta-tsai for parent carries out combo, again through too much generation backcrosses, purple Wuta-tsai kind can be selected completely.
The present invention is by the following technical solutions:
Be maternal by the aubergine Chinese cabbage of inheritance stability, with Wuta-tsai hybridization between selfed lines, obtain purple F1 plant, be that male parent backcrosses with Wuta-tsai selfing again, purple plant Wuta-tsai inbred line continuous backcross is selected in conjunction with field test, obtain the Wuta-tsai backcross plant of purple, then obtain the stable purple Wuta-tsai material of hereditary capacity by selfing, specifically comprise:
1) the purple Chinese cabbage PC601 obtaining inheritance stability is selected;
2) select to obtain the excellent Wuta-tsai inbred line YW905 of Comprehensive Traits;
3) with purple Chinese cabbage PC601 for female parent, with Wuta-tsai inbred line YW905 for male parent is hybridized, obtain F1 plant;
4) select purple F1 plant to do maternal, with above-mentioned Wuta-tsai inbred line YW905 male parent, backcross and obtain BC1 seed;
5) field test is carried out with BC1 seed, pass through field observation, principal character of selecting and remain characteristic shows the backcross female parent of the purple fine individual plant similar to transformation male parent as next round, continue to backcross with above-mentioned Wuta-tsai inbred line YW905 male parent, iterate through field test select and continuous backcross acquisition Comprehensive Traits close to Wuta-tsai inbred line male parent, the BCn plant of blade purple;
6) purple BCn plant is carried out selfing and obtain seed BCnS1;
7) BCnS1 is selected in conjunction with economical character many generation through field test, obtain the Wuta-tsai plant that hereditary capacity stabilization vane look is purple;
8) the purple Wuta-tsai plant of acquisition is continued the Wuta-tsai seed that a large amount of purple of numerous acquisition is expanded in selfing.
The described step 5) number of times that backcrosses is 3-6 time.
Purple Chinese cabbage PC601 in the present invention and Wuta-tsai inbred line YW905 two kinds of germ plasm resources are all kept in Institute of Gardening, Anhui Academy of Agricultural Sciences's species bank.Wherein purple Chinese cabbage derives from the purple Chinese cabbage variant found in Institute of Gardening, Anhui Academy of Agricultural Sciences's variety source, by preferably involving continuous individual plant selfing purifying in a criminal case, the a series of deep mixed strain obtained, select the further selfing purifying of strain that color is more gorgeous, obtain the purple Chinese cabbage homozygous inbred lines PC601 of offspring without trait segregation; The Anhui Native Wuta-tsai resource six of collection is pacified gold zone crow by 3-5 for continuous selfing, the homozygous inbred lines of the stabilization characteristics of genetics of acquisition by Wuta-tsai inbred line YW905.
Accompanying drawing explanation
Fig. 1 is the breeding line spectrogram of purple Wuta-tsai of the present invention.
Embodiment
Following examples and accompanying drawing for illustration of the present invention, but are not used for limiting the scope of the invention.
The seed selection of purple Wuta-tsai comprises the transformation of the collection of resource, parent's seed selection, blade purple gene.
Parent's seed selection: the purple Chinese cabbage variant found in female parent selection Institute of Gardening, Anhui Academy of Agricultural Sciences variety source, by preferably involving continuous individual plant selfing purifying in a criminal case, the a series of deep mixed strain obtained, select the further selfing purifying of strain that color is more gorgeous, obtain the purple Chinese cabbage homozygous inbred lines PC601 of offspring without trait segregation, this strain plant height 18-25cm, degree of development 26-30cm, blade aubergine, blade back petiole is light green, leaf ellipse.
Male parent selects Anhui Native Wuta-tsai farm variety Hefei gold zone crow, by 3 generations selfing purifying, obtain the homozygous inbred lines YW905 of offspring without trait segregation, this strain plant half is upright, plant height 15-17cm, degree of development 18-19cm, blade obovate, there is blister gauffer on blade face, outer leaf green, lobus cardiacus is yellow time ripe, petiole white, Weight per plant about 0.5kg.In ripe, cold-resistant thermo-labile, more drought-enduring, waterlogging, disease and insect resistance is strong, and fiber is few, and quality is tender, and local flavor is good, more delicious through frost and snow aftertaste.
The transformation of blade purple gene: with purple Chinese cabbage PC601 for female parent, Wuta-tsai inbred line YW905 is that male parent is hybridized, carry out artificial bud pollination, male parent is one day bagging before flowering, in blooming, the same day gets pollen, and maternal flower bud phase stripping flower bud, removes stamen, male parent fresh pollen is applied in maternal bud column cap, and makes marks.The seed obtained after the bud awarding powder bears pods maturation is F1 generation seed;
Gone down by the F1 planting seed of acquisition, the purple F1 plant that obtains does maternal, with Wuta-tsai inbred line YW905 for male parent backcrosses acquisition (YW905) first backcross generation (BC1) seed;
(YW905) first backcross generation (BC1) seed obtained is carried out field test, selects purple plant to do maternal, with Wuta-tsai inbred line YW905 for male parent continuous backcross obtains (YW905) second backcross generation (BC2) seed;
(YW905) second backcross generation (BC2) seed obtained is carried out field test, purple plant is selected to do maternal, with Wuta-tsai inbred line YW905 for male parent continuous backcross, to be selected by field and continuous backcross acquisition Comprehensive Traits to backcross four generations (BC4) plant close to (YW905) that Wuta-tsai inbred line YW905, leaf look are purple.
Four generations (BC4) plant that backcrossed by the purple obtained (YW905) carries out selfing acquisition (YW905) BC4S1;
(YW905) BC4S1 selfed seed obtained is carried out field test, selects to obtain that hereditary capacity is stable, plant color shows as purple, other proterties and the close purple Wuta-tsai plant of Wuta-tsai inbred line YW905.By identifying that (YW905) BC4S1 purple Wuta-tsai plant selfing obtained is expanded numerous, obtain a large amount of seed PW1301 of stable purple Wuta-tsai (YW905) BC4S1 of hereditary capacity.
The purple Wuta-tsai inbred line PW1301 plant be bred as half is upright, plant height 15-18cm, degree of development 18-20cm, blade obovate, and there is blister gauffer on blade face, siphonal lobe purple, aubergine when lobus cardiacus is ripe, and the green white of petiole is more flat, Weight per plant about 0.5kg.In ripe, cold-resistant, more drought-enduring, waterlogging, disease resistance is strong, and fiber is few, and quality is tender, and local flavor is good, through frost and snow aftertaste more delicious (table 1, table 2).
Table 1 different cultivars compares breeding time
The disease-resistant resistance of table 2 different cultivars compares
Cold-resistant, Resistance screening method is: Adult plant investigation plant by freeze injury degree and plant downy mildew, the virus disease incidence of disease.
Cold-resistance index=∑ (the cold-resistant rank of k*) * 100/5* investigates total strain number; K is appropriate level strain number.
Cold-resistant grade scale:
0 grade, there is not manifest symptom in normal plants;
1 grade, leaf color jade green, a small amount of (1) siphonal lobe is slightly wilted;
2 grades, 2-3 sheet siphonal lobe is slightly wilted;
3 grades, more than 3 outer leafs are wilted;
4 grades, internal lobe is softening wilts, but can recover;
5 grades, inside there is softening wilting, irrecoverable.
Cold-resistant evaluation criterion: cold-resistant: cold-resistance index (0-5): in anti-: cold-resistance index (5-10): cold-resistant: cold-resistance index (10-20): responsive: cold-resistance index (20-100).
Claims (1)
1. a selection for purple Wuta-tsai, is characterized in that carrying out as follows:
1) the purple Chinese cabbage PC601 obtaining inheritance stability is selected;
2) select to obtain the excellent Wuta-tsai inbred line YW905 of Comprehensive Traits;
3) with purple Chinese cabbage PC601 for female parent, with Wuta-tsai inbred line YW905 for male parent is hybridized, obtain F1 plant;
4) select purple F1 plant to do maternal, with above-mentioned Wuta-tsai inbred line YW905 male parent, backcross and obtain BC1 seed;
5) field test is carried out with BC1 seed, pass through field observation, principal character of selecting and remain characteristic shows the backcross female parent of the purple fine individual plant similar to transformation male parent as next round, continue to backcross with above-mentioned Wuta-tsai inbred line YW905 male parent, iterate through field test select and continuous backcross acquisition Comprehensive Traits close to Wuta-tsai inbred line male parent, the BCn plant of blade purple;
6) purple BCn plant is carried out selfing and obtain seed BCnS1;
7) BCnS1 selfed seed is carried out field test, select to obtain that hereditary capacity is stable, plant color shows as purple, other proterties and the close purple Wuta-tsai plant of Wuta-tsai inbred line YW905;
8) the purple Wuta-tsai plant of acquisition is continued the Wuta-tsai seed that a large amount of purple of numerous acquisition is expanded in selfing;
Described step 5) number of times that backcrosses is 3-6 time.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104521740B (en) * | 2014-12-17 | 2017-01-11 | 安徽农业大学 | Synchronous transform-breeding method for wuta-cai homozygous inbred line and cytoplasmic male sterile line |
| CN105815211A (en) * | 2016-03-26 | 2016-08-03 | 安徽科技学院 | Method for improving self-incompatibility Wuta-tsai hybridization efficiency |
| CN106613898A (en) * | 2016-09-21 | 2017-05-10 | 韶关学院 | Breeding method of Wuta-tsai cytoplasmic male sterile line and maintainer line |
| CN106258941A (en) * | 2016-09-21 | 2017-01-04 | 韶关学院 | The selection-breeding of a kind of Wuta-tsai and propagation method |
| CN109156337A (en) * | 2018-09-18 | 2019-01-08 | 贵州省油菜研究所 | A kind of ornamental rape flower selection |
| CN112056207A (en) * | 2020-09-29 | 2020-12-11 | 南京新创蔬菜分子育种研究院有限公司 | Breeding method of green stem black-bone vegetable material |
| CN116616170A (en) * | 2023-07-14 | 2023-08-22 | 安徽省华禾种业有限公司 | Breeding method for maintaining variety diversity of Wuta-tsai |
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| CN101416607A (en) * | 2008-12-08 | 2009-04-29 | 中国农业科学院蔬菜花卉研究所 | Claret Chinese cabbage breeding method |
| CN102577819A (en) * | 2012-03-17 | 2012-07-18 | 常熟市尚湖农业生态园有限公司 | High-yield cultivation method for Brassica narinosa |
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| CN101416607A (en) * | 2008-12-08 | 2009-04-29 | 中国农业科学院蔬菜花卉研究所 | Claret Chinese cabbage breeding method |
| CN102577819A (en) * | 2012-03-17 | 2012-07-18 | 常熟市尚湖农业生态园有限公司 | High-yield cultivation method for Brassica narinosa |
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