CN104663423A - Method for breeding thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S - Google Patents

Method for breeding thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S Download PDF

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Publication number
CN104663423A
CN104663423A CN201510061292.4A CN201510061292A CN104663423A CN 104663423 A CN104663423 A CN 104663423A CN 201510061292 A CN201510061292 A CN 201510061292A CN 104663423 A CN104663423 A CN 104663423A
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breeding
generation
male sterile
hainan
extensively
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吴险峰
方忠坤
张爱国
吴峰峰
宁会琴
杨振林
吴菁菁
李根明
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Guangde County's Institute Of Agricultural Sciences
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Guangde County's Institute Of Agricultural Sciences
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Abstract

The invention discloses a method for breeding a thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S. The method for breeding the thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S comprises the following steps: (1) taking Guangzhan 63S as a female parent and Guanghui7849 as a male parent; (2) hybridizing to obtain a hybrid F1, and mixing and harvesting a small amount of F1 seeds; (3) planting F2, selecting 12 low-panicle-enclosure and typical abortion and sterile plants to obtain F3 seeds, and planting F3 in 12 plant rows; (4) selecting three single plant line groups, cutting stubbles, and regenerating to obtain selfing seeds F4; (5) carrying out generation adding to obtain F5; (6) adopting shuttle breeding, namely south-reproduction and north-selection, from a generation F4, and synchronously breeding male sterile lines in a generation F8, wherein a male sterile line generation reaches up to F12 and is named as Guangxuan S. Rice quality of Guang18S obtained by adopting the method disclosed by the invention is as follows: coarse rice rate is 81.39%, milled rice rate is 74.4%, head milled rice rate is 71.3%, chalky kernel percentage is 21%, chalk degree is 1.5%, transparency is 1%, and grain length is 6.5mm.

Description

The temperature sensitive dual purpose genic male sterile line of a kind of light extensively selects the selection of S
Technical field:
The present invention relates to the selection that the temperature sensitive dual purpose genic male sterile line of a kind of light extensively selects S, belong to agricultural technology field.
Background technology:
Hybrid rice is produced by different seed rice phase mutual cross, dependence be maternal and its two objects, i.e. " three are " (male sterile line, maintainer and restorer).Production will be planted one piece of breeding field and one piece of seed farm; breeding field plantation male sterile line and maintainer; when they are all bloomed time; maintainer sends pollen to male sterile line by means of media such as wind, worm, water, birds; it is solid that male sterile line obtains normal pollen; the offspring produced remains male sterile line, reaches breeding male sterile lines object.The male-sterile seed that breeding can come by we, retain a part and continue breeding the coming year, another part is then with the restorer production of hybrid seeds, when the male sterile line of seed farm and restorer are all bloomed time, the pollen of restorer sends male sterile line to, the offspring that male sterile line produces, is just to provide the hybrid rice seed of field planting.
Existing hybrid rice mainly solves a difficult problem for high yield, but the problem of ubiquity rice quality difference.Along with the living standard of people improves day by day, meal of having enough can not satisfy the demands of consumers, and therefore seed selection high-quality rice variety is brought into schedule, becomes a research focus of current rice science men.And conventional rice quality better but yield poorly, therefore not only high yield but also high-quality are conventional Rice and existing hybrid rice breeding all open questions.
Summary of the invention:
The present invention seeks to, be used for making up the deficiencies in the prior art, and provide the temperature sensitive dual purpose genic male sterile line of a kind of light extensively to select the selection of S.
To achieve these goals, technical scheme of the present invention is as follows:
The temperature sensitive dual purpose genic male sterile line of light extensively selects a selection of S, comprises following step:
(1) take Guangzhan 63S as female parent, extensively extensive 7849 is male parent;
(2) Hybrids F1 is obtained in Sanya, Hainan hybridization, a small amount of F1 kind of mixed receipts;
(3) low, the typical abortion of bag neck degree is selected at Guangde County Anhui Province plantation F2, sterile plant 12 strain, carries out Ratooning reproduction shading in mid or late August and carries out self-fertility, selects the good individual plant of fertility restorer, obtain F3 seed, then Sanya, Hainan plantation in winter F3 generation 12 plants;
(4) select economical character better, the strain 8 of sword-like leave straight elevator, nick; Carry out economical character, the Fertility observation of male sterile line, select that agronomy present situation, regularity are relatively consistent, Sterile stability is excellent and the numbering A that flowering habit is good, B, C tri-colonies of individual plant system, mid-August then, Ratooning reproduction, obtains selfed seed F4;
(5) adding for obtaining F5 at Sanya, Hainan, is that in-group's economical character is basicly stable, and wherein numbering GS11-13 economical character is better, and the most obvious in sterile period abortion, Hainan winter is numerous solid higher;
(6) start from F4 generation, in Sanya, Hainan Guangde County Anhui Province two places, adopt shuttle breeding, Nan Fanbei educates, and synchronously carries out sterile line breeding to F8 generation, selection pressure under increasing natural conditions, carry out manually being measured of a small amount of combination, a small amount of production of core seeds, the packaged combination isolation production of hybrid seeds; Male sterile line reaches F12 from generation to generation, and called after extensively selects S.
Wide 18S interval sowing fertility under the natural conditions of Guangde dynamically shows:
Within 2005,2006, extensively select S at Guangde interval sowing, it is dynamic to observe its fertility under field conditions (factors).Within 2005, sow first phase from May 1 to June 10 every nearly 10 days, sowed for 5 phases altogether; Within 2006, sow first phase from April 30 to June 10 every nearly 10 days, sowed for 5 phases altogether; Each issue from beginning fringe, every 3-4 days microscopies, a small amount of bagging in interval, observed pollen abortion degree and bagging self-fruitful rate.The results are shown in Table 1.
Table 1
Within 2005, extensively select S all to show as 100% during beginning fringe to August 26 from July 25 and lose type abortion without powder or few pollen allusion quotation, bagging self-fruitful rate is 0, and the sterile phase is longer than 30 days.Due to by August 17 to August 19 for three days on end daily mean temperature lower than the low temperature overcast and rainy day weather effect (wherein August 18 samming only 20 degree) of 23.50C.After entering low temperature, to extensively selecting S to carry out continuous microscopy, each microscopy 20-30 fringe.Microscopy finds extensively to select S from 28-September 3 August (the 13rd day namely after first day low temperature) male sterile line by a small amount of typical abortion type abortion, transferring to has illicit sexual relations loses and the appearance of fertile flower powder, look into bagging and have self-fertility, wherein with August 31 pollen abortion rate for 92.00%, bagging self-fruitful rate is 5.6%.Microscopy performance abortion on September 5, bagging self-fruitful rate is 0.Within 2006, extensively select S microscopy and bagging selfing in July 26, beginning fringe was continuous to Nikkei in August 30 next day, all show as 100% without pollen or few typical abortion type abortion and bagging without self-fertility phenomenon.Within 2007, extensively select S in day by day fringe microscopy in August 3, daily show as 100% without pollen to August 18.Naturally observing for many years shows, extensively selects S to have stable sterility and is longer than the steady sterile period of 30 days.
Extensively select S in annual sowing mid-November of Sanya, Hainan to early March next year, fringe beginning, under the condition of short day low temperature, generally solid between 65%-80%, have stable fertility in Hainan, breeding is conducive to improving output during this period.Along with sowing is postponed, fringe phase beginning in the heading of late March-early April, fertility progressively by can educate transfer to sterile, after April 15, pollen stamen abortion.Extensively select S at Guangde County Anhui Province, Ratooning reproduction, to front and back beginning fringe on September 25, by the impact of light and temperature, regenerated ripening rate between 30-60%, had obvious Fertility al-teration from sterile to educating August 25.
Extensively select the Growing Characters record of S in table 2.Show breeding time extensively to select S to have more stable duration from seeding to heading and the stable number of blade, be beneficial to the arrangement of seed production florescence.
2005 Guangde area, extensively select S in May 1 sowing, July 25 beginning fringe, duration from seeding to heading is 85 days, leaf number of the main culm is 13.6 leaves, along with date of seeding postpones, and corresponding shortening breeding time, it is 85-72 days that duration from seeding to heading becomes spoke, and leaf number of the main culm is relatively stable, and luffing is 13.4-13.7 leaf.
Within 2006, extensively select S in sowing on April 30, July 26 beginning fringe, duration from seeding to heading is 87 days, and leaf number of the main culm is 13.5 leaves, wherein the sowing of phases on May 10 to June 10 4, and its duration from seeding to heading luffing is 83-71 days, and leaf number of the main culm luffing is 13.8-13.4 leaf.
Table 2
S Guangde is extensively selected to plant sterile phase plant height 86 ± 5cm, spike length average out to 24.5 ± 3cm, effective ears 9-12, every total grain panicle number 168 ± 10; Extensively select S column cap colourless, stigma exposing ratio is general, and fringe base portion has slight bag neck, and responsive to " 920 ", outcrossing is general.Phase plant height 81 ± 5cm can be educated in Hainan, spike length 23.5 ± 3cm, Earsper plant 9-13, every total grain panicle number 158 ± 10, thousand kernel weight 28 ± 0.5 grams.Extensively select long, narrow straight elevator nick in S blade, leaf look green, and plant type is good, stem stalk is sturdy.
Beneficial effect of the present invention is as follows:
The wide 18S rice quality that selection of the present invention obtains: Coarse Rice Rate 81.39%, polished rice rate 74.4%, head rice rate 71.3%, chalky grain rate 21%, chalkiness degree 1.5%, transparency 1%, grain length 6.5mm, aspect ratio 2:7,8 indexs reach superior rice standard.Be that MS (5) and bacterial leaf-blight show as MR (3) to rice blast resistance.
Embodiment:
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
Embodiment 1
The temperature sensitive dual purpose genic male sterile line of light extensively selects a selection of S, comprises following step:
(1) take Guangzhan 63S as female parent, extensively extensive 7849 is male parent;
(2) Hybrids F1 is obtained in Sanya, Hainan hybridization, a small amount of F1 kind of mixed receipts;
(3) low, the typical abortion of bag neck degree is selected at Guangde County Anhui Province plantation F2, sterile plant 12 strain, carries out Ratooning reproduction shading in mid or late August and carries out self-fertility, selects the good individual plant of fertility restorer, obtain F3 seed, then Sanya, Hainan plantation in winter F3 generation 12 plants;
(4) select economical character better, the strain 8 of sword-like leave straight elevator, nick; Carry out economical character, the Fertility observation of male sterile line, select that agronomy present situation, regularity are relatively consistent, Sterile stability is excellent and the numbering A that flowering habit is good, B, C tri-colonies of individual plant system, mid-August then, Ratooning reproduction, obtains selfed seed F4;
(5) adding for obtaining F5 at Sanya, Hainan, is that in-group's economical character is basicly stable, and wherein numbering GS11-13 economical character is better, and the most obvious in sterile period abortion, Hainan winter is numerous solid higher;
(6) start from F4 generation, in Sanya, Hainan Guangde County Anhui Province two places, adopt shuttle breeding, Nan Fanbei educates, and synchronously carries out sterile line breeding to F8 generation, selection pressure under increasing natural conditions, carry out manually being measured of a small amount of combination, a small amount of production of core seeds, the packaged combination isolation production of hybrid seeds; Male sterile line reaches F12 from generation to generation, and called after extensively selects S.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.

Claims (1)

1. the temperature sensitive dual purpose genic male sterile line of light extensively selects a selection of S, it is characterized in that, comprises following step: (1) take Guangzhan 63S as female parent, and extensively extensive 7849 is male parent;
(2) Hybrids F1 is obtained in Sanya, Hainan hybridization, a small amount of F1 kind of mixed receipts;
(3) low, the typical abortion of bag neck degree is selected at Guangde County Anhui Province plantation F2, sterile plant 12 strain, carries out Ratooning reproduction shading in mid or late August and carries out self-fertility, selects the good individual plant of fertility restorer, obtain F3 seed, then Sanya, Hainan plantation in winter F3 generation 12 plants;
(4) select economical character better, the strain 8 of sword-like leave straight elevator, nick; Carry out economical character, the Fertility observation of male sterile line, select that agronomy present situation, regularity are relatively consistent, Sterile stability is excellent and the numbering A that flowering habit is good, B, C tri-colonies of individual plant system, mid-August then, Ratooning reproduction, obtains selfed seed F4;
(5) adding for obtaining F5 at Sanya, Hainan, is that in-group's economical character is basicly stable, and wherein numbering GS11-13 economical character is better, and the most obvious in sterile period abortion, Hainan winter is numerous solid higher;
(6) start from F4 generation, in Sanya, Hainan Guangde County Anhui Province two places, adopt shuttle breeding, Nan Fanbei educates, and synchronously carries out sterile line breeding to F8 generation, selection pressure under increasing natural conditions, carry out manually being measured of a small amount of combination, a small amount of production of core seeds, the packaged combination isolation production of hybrid seeds; Male sterile line reaches F12 from generation to generation, and called after extensively selects S.
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Cited By (8)

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CN105475121A (en) * 2016-01-29 2016-04-13 重庆市农业科学院 Breeding method for high-temperature-resistant and disease-resistant rice two-line sterile line
CN105638446A (en) * 2016-03-16 2016-06-08 湖南省水稻研究所 Method for breeding rice dual-purpose genic male sterile line with excellent seed production characteristics
CN106856966A (en) * 2017-04-10 2017-06-20 河北省农林科学院旱作农业研究所 A kind of hybrid selection method of photoperiod-sensitive type sudangrass
CN106954545A (en) * 2017-03-02 2017-07-18 安徽金培因科技有限公司 A kind of is the breeding method of line with genic sterile indica hybrid rice for light temperature sensitive two
CN107801629A (en) * 2017-11-01 2018-03-16 安徽友鑫农业科技有限公司 A kind of round-grained rice two is the selection of temperature and light sensitive nuclear sterility system
CN108834878A (en) * 2018-07-17 2018-11-20 安徽新安种业有限公司 The breeding method of excellent Indica TGMS line rice
CN110679477A (en) * 2019-11-15 2020-01-14 河北省农林科学院旱作农业研究所 Breeding method of photoperiod strong-sensitivity BMR sorghum sterile line
CN112753564A (en) * 2020-12-29 2021-05-07 湖南省春云农业科技股份有限公司 Breeding method of high-quality two-line sterile line of rice

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CN103598085A (en) * 2013-10-28 2014-02-26 江苏沿海地区农业科学研究所 Breeding method of photo-thermo-sensitive genic male sterile line of quality rice

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CN103598085A (en) * 2013-10-28 2014-02-26 江苏沿海地区农业科学研究所 Breeding method of photo-thermo-sensitive genic male sterile line of quality rice

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105475121A (en) * 2016-01-29 2016-04-13 重庆市农业科学院 Breeding method for high-temperature-resistant and disease-resistant rice two-line sterile line
CN105475121B (en) * 2016-01-29 2018-10-26 重庆市农业科学院 A kind of selection of high temperature resistant disease resisting rice two-line sterile line
CN105638446A (en) * 2016-03-16 2016-06-08 湖南省水稻研究所 Method for breeding rice dual-purpose genic male sterile line with excellent seed production characteristics
CN106954545A (en) * 2017-03-02 2017-07-18 安徽金培因科技有限公司 A kind of is the breeding method of line with genic sterile indica hybrid rice for light temperature sensitive two
CN106856966A (en) * 2017-04-10 2017-06-20 河北省农林科学院旱作农业研究所 A kind of hybrid selection method of photoperiod-sensitive type sudangrass
CN106856966B (en) * 2017-04-10 2019-11-05 河北省农林科学院旱作农业研究所 A kind of hybrid selection method of photoperiod-sensitive type sudangrass
CN107801629A (en) * 2017-11-01 2018-03-16 安徽友鑫农业科技有限公司 A kind of round-grained rice two is the selection of temperature and light sensitive nuclear sterility system
CN107801629B (en) * 2017-11-01 2021-01-15 安徽友鑫农业科技有限公司 Breeding method of japonica two-line temperature-sensitive genic male sterile line
CN108834878A (en) * 2018-07-17 2018-11-20 安徽新安种业有限公司 The breeding method of excellent Indica TGMS line rice
CN110679477A (en) * 2019-11-15 2020-01-14 河北省农林科学院旱作农业研究所 Breeding method of photoperiod strong-sensitivity BMR sorghum sterile line
CN112753564A (en) * 2020-12-29 2021-05-07 湖南省春云农业科技股份有限公司 Breeding method of high-quality two-line sterile line of rice

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Application publication date: 20150603