CN104663424A - Method for breeding rice thermo-photoperiod sensitive dual-purpose genic male sterile - Google Patents
Method for breeding rice thermo-photoperiod sensitive dual-purpose genic male sterile Download PDFInfo
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- CN104663424A CN104663424A CN201510061334.4A CN201510061334A CN104663424A CN 104663424 A CN104663424 A CN 104663424A CN 201510061334 A CN201510061334 A CN 201510061334A CN 104663424 A CN104663424 A CN 104663424A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 23
- 235000009566 rice Nutrition 0.000 title claims abstract description 23
- 238000009395 breeding Methods 0.000 title claims abstract description 16
- 230000001488 breeding effect Effects 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title abstract 2
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims abstract description 24
- 241000209094 Oryza Species 0.000 claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 claims abstract description 12
- 230000035558 fertility Effects 0.000 claims abstract description 6
- 238000002955 isolation Methods 0.000 claims abstract description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 4
- 206010000210 abortion Diseases 0.000 claims description 11
- 231100000176 abortion Toxicity 0.000 claims description 11
- 230000009977 dual effect Effects 0.000 claims description 6
- 238000009340 sequential cropping Methods 0.000 claims description 6
- 101000708283 Oryza sativa subsp. indica Protein Rf1, mitochondrial Proteins 0.000 claims description 3
- 238000009405 line breeding Methods 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 abstract 2
- 230000001172 regenerating effect Effects 0.000 abstract 2
- 238000000386 microscopy Methods 0.000 description 7
- 238000010899 nucleation Methods 0.000 description 6
- 238000009331 sowing Methods 0.000 description 5
- 230000008774 maternal effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000036461 convulsion Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009401 outcrossing Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention discloses a method for breeding a rice thermo-photoperiod sensitive dual-purpose genic male sterile. The rice thermo-photoperiod sensitive dual-purpose genic male sterile comprises the following steps: (1) taking Guangzhan 63S as a female parent and Guanghui499 as a male parent; (2) hybridizing and combining the Guangzhan 63S and Guanghui499 to obtain Guangliangyou 4 hybrid F1, mixing and harvesting a small amount of F1 seeds; (3) planting F2, selecting 12 plants, cutting stubbles and regenerating for carrying out self seed-setting, mixing and harvesting single plants with good restoring fertility to obtain F3 seeds, planting F3 in the same year to obtain 83 mature seedlings; (4) selecting 28 single plant lines, cutting stubbles of the single plant lines with relatively consistent agricultural current conditions and uniformity, excellent sterile stability and good flowering habit in the middle ten days of August, and regenerating to obtain selfing seeds F5; (5) adopting shuttle breeding, namely south-reproduction and north-selection, from a generation F4, synchronously breeding male sterile lines in a generation F8, producing a small amount of core seeds, and carrying out small-scale combination and isolation for seed production, wherein a male sterile line generation reaches up to F10 and is named as Guang18S.
Description
Technical field:
The present invention relates to a kind of selection of rice photo-thermo-sensitive dual purpose genic male sterile line, belong to agricultural technology field.
Background technology:
Hybrid rice is produced by different seed rice phase mutual cross, dependence be maternal and its two objects, i.e. " three are " (male sterile line, maintainer and restorer).Production will be planted one piece of breeding field and one piece of seed farm; breeding field plantation male sterile line and maintainer; when they are all bloomed time; maintainer sends pollen to male sterile line by means of media such as wind, worm, water, birds; it is solid that male sterile line obtains normal pollen; the offspring produced remains male sterile line, reaches breeding male sterile lines object.The male-sterile seed that breeding can come by we, retain a part and continue breeding the coming year, another part is then with the restorer production of hybrid seeds, when the male sterile line of seed farm and restorer are all bloomed time, the pollen of restorer sends male sterile line to, the offspring that male sterile line produces, is just to provide the hybrid rice seed of field planting.
Existing hybrid rice mainly solves a difficult problem for high yield, but the problem of ubiquity rice quality difference.Along with the living standard of people improves day by day, meal of having enough can not satisfy the demands of consumers, and therefore seed selection high-quality rice variety is brought into schedule, becomes a research focus of current rice science men.And conventional rice quality better but yield poorly, therefore not only high yield but also high-quality are conventional Rice and existing hybrid rice breeding all open questions.
Summary of the invention:
The present invention seeks to, be used for making up the deficiencies in the prior art, and a kind of selection of rice photo-thermo-sensitive dual purpose genic male sterile line is provided.
To achieve these goals, technical scheme of the present invention is as follows:
A selection for rice photo-thermo-sensitive dual purpose genic male sterile line, comprises following step:
(1) with the Guangzhan 63S of selection cross for maternal homophyletic is leaf excellent, there is Xian round-grained rice and hand over extensively extensive 499 of relationship to be that the production of hybrid seeds of male parent is combined;
(2) at wide two excellent No. 4 Hybrids F1 of double-line hybrid combination, a small amount of F1 kind of mixed receipts;
(3) low, the typical abortion of bag neck degree is selected at Guangde County Anhui Province plantation F2, sterile plant 12 strain, carries out Ratooning reproduction in mid or late August and carries out self-fertility, selects the mixed receipts of the good individual plant of fertility restorer, obtain F3 seed, Sanya, Hainan plantation in winter F3 is for seedling 83 strain then;
(4) select economical character better, sword-like leave straight elevator, 28, individual plant system, with parents' group number code name 6349S; Carry out economical character, the Fertility observation of male sterile line, selects that agronomy present situation, regularity are relatively consistent, Sterile stability is excellent and the numbering A1 that flowering habit is good, A2, A3, A4, A5 colony of individual plant system performance are better, mid-August then, Ratooning reproduction, obtains selfed seed F5;
(5) start from F4 generation, in Sanya, Hainan Guangde County Anhui Province two places, adopt shuttle breeding, Nan Fanbei educates.Synchronously carry out sterile line breeding to F8 generation, selection pressure under increasing natural conditions, carries out manually being measured of a small amount of combination, a small amount of production of core seeds, the packaged combination isolation production of hybrid seeds; Male sterile line reaches F10, the wide 18S of called after from generation to generation.
Wide 18S interval sowing fertility under the natural conditions of Guangde dynamically shows:
Within 2011,2012, at the wide 18S of Guangde interval sowing, it is dynamic to observe its fertility under field conditions (factors).Within 2011, sow first phase from May 1 to June 10 every nearly 10 days, sowed for 5 phases altogether; Within 2012, sow first phase from April 30 to June 10 every nearly 10 days, sowed for 5 phases altogether; Each issue from beginning fringe, every 3-4 days microscopies, a small amount of bagging in interval, observed pollen abortion degree and bagging self-fruitful rate.The results are shown in Table 1.
Table 1
Wide 18S in 2011 all showed as 100% from July 25 and loses type abortion without powder or few pollen allusion quotation during beginning fringe to August 26, bagging self-fruitful rate is 0, and the sterile phase is longer than 30 days.After entering low temperature, continuous microscopy is carried out to wide 18S, each microscopy 10-20 fringe.Microscopy finds wide 18S from 28 ~ September 3 August (the 13rd day namely after first day low temperature) male sterile line by a small amount of typical abortion type abortion, transferring to has illicit sexual relations loses and the appearance of fertile flower powder, look into bagging and have self-fertility, wherein with August 31 pollen abortion rate for 92.00%, bagging self-fruitful rate is 5.6%.Microscopy performance abortion on September 5, bagging self-fruitful rate is 0.Wide 18S microscopy and bagging selfing in July 26, beginning fringe was continuous to Nikkei in August 30 next day in 2012, all show as 100% without pollen or few typical abortion type abortion and bagging without self-fertility phenomenon.Within 2012, wide 18S is in day by day fringe microscopy in August 3, daily shows as 100% without pollen to August 18.Naturally observing for many years shows, wide 18S has stable sterility and is longer than the steady sterile period of 30 days.
The Growing Characters record of wide 18S is in table 2.Show that wide 18S has more stable duration from seeding to heading and the stable number of blade breeding time, be beneficial to the arrangement of seed production florescence.
2011 Guangde area, wide 18S in May 1 sowing, July 25 beginning fringe, duration from seeding to heading is 85 days, leaf number of the main culm is 13.6 leaves, along with date of seeding postpones, and corresponding shortening breeding time, it is 85-72 days that duration from seeding to heading becomes spoke, and leaf number of the main culm is relatively stable, and luffing is 13.4-13.7 leaf.
Wide 18S in 2012 in sowing on April 30, July 26 beginning fringe, duration from seeding to heading is 87 days, and leaf number of the main culm is 13.5 leaves, and wherein May 10 is to 4 phases sowing on June 10,
Its duration from seeding to heading luffing is 83-71 days, and leaf number of the main culm luffing is 13.8-13.4 leaf.
Table 2
The sterile phase plant height 87cm of wide 18S Guangde plantation, spike length average out to 24.5cm, effective ears 9-12, every total grain panicle number 185; Wide 18S column cap is colourless, and stigma exposing ratio is better, and fringe base portion has slight bag neck, and responsive to " 920 ", outcrossing is better.Phase plant height 81cm can be educated in Hainan, spike length 23.5cm, Earsper plant 9.5, every total grain panicle number 158, thousand kernel weight 28 grams.Long in wide 18S blade, narrow straight elevator nick, leaf look pale green, plant type loose moderate, stem stalk is sturdy.
Compared by paired observation, the main distinction of wide 18S and Guangzhan 63S is in table 3.
Table 3
Beneficial effect of the present invention is as follows:
The wide 18S that selection of the present invention obtains, plant height 81.6cm, plant type degree of tightness is moderate, and tillering ability is medium, sword-like leave clean and jerk, middle length, narrow, open and flat, leaf look pale green, bran point and column cap colourless, proterties neat and consistent, sterile plant rate 100%.This male sterile line 5cm more than shorter than Guangzhan 63S plant, sword-like leave is obviously greater than with stem angle and contrasts, thousand kernel weight height 2g.Comprehensive Traits is more excellent, economical character neat and consistent, and sterility is stablized, and reaches rice sterile line criterion for breeding.
Embodiment:
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
Embodiment 1
A selection for rice photo-thermo-sensitive dual purpose genic male sterile line, comprises following step:
(1) with the Guangzhan 63S of selection cross for maternal homophyletic is leaf excellent, there is Xian round-grained rice and hand over extensively extensive 499 of relationship to be that the production of hybrid seeds of male parent is combined;
(2) at wide two excellent No. 4 Hybrids F1 of double-line hybrid combination, a small amount of F1 kind of mixed receipts;
(3) low, the typical abortion of bag neck degree is selected at Guangde County Anhui Province plantation F2, sterile plant 12 strain, carries out Ratooning reproduction in mid or late August and carries out self-fertility, selects the mixed receipts of the good individual plant of fertility restorer, obtain F3 seed, Sanya, Hainan plantation in winter F3 is for seedling 83 strain then;
(4) select economical character better, sword-like leave straight elevator, 28, individual plant system, with parents' group number code name 6349S; Carry out economical character, the Fertility observation of male sterile line, selects that agronomy present situation, regularity are relatively consistent, Sterile stability is excellent and the numbering A1 that flowering habit is good, A2, A3, A4, A5 colony of individual plant system performance are better, mid-August then, Ratooning reproduction, obtains selfed seed F5;
(5) start from F4 generation, in Sanya, Hainan Guangde County Anhui Province two places, adopt shuttle breeding, Nan Fanbei educates.Synchronously carry out sterile line breeding to F8 generation, selection pressure under increasing natural conditions, carries out manually being measured of a small amount of combination, a small amount of production of core seeds, the packaged combination isolation production of hybrid seeds; Male sterile line reaches F10, the wide 18S of called after from generation to generation.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (1)
1. a selection for rice photo-thermo-sensitive dual purpose genic male sterile line, is characterized in that, comprises following step:
(1) with the Guangzhan 63S of selection cross for female parent, there is Xian round-grained rice and hand over extensively extensive 499 of relationship for male parent;
(2) at wide two excellent No. 4 Hybrids F1 of double-line hybrid combination, a small amount of F1 kind of mixed receipts;
(3) low, the typical abortion of bag neck degree is selected at Guangde County Anhui Province plantation F2, sterile plant 12 strain, carries out Ratooning reproduction in mid or late August and carries out self-fertility, selects the mixed receipts of the good individual plant of fertility restorer, obtain F3 seed, Sanya, Hainan plantation in winter F3 is for seedling 83 strain then;
(4) select economical character better, sword-like leave straight elevator, 28, individual plant system, with parents' group number code name 6349S; Carry out economical character, the Fertility observation of male sterile line, selects that agronomy present situation, regularity are relatively consistent, Sterile stability is excellent and the numbering A1 that flowering habit is good, A2, A3, A4, A5 colony of individual plant system performance are better, mid-August then, Ratooning reproduction, obtains selfed seed F5;
(5) start from F4 generation, in Sanya, Hainan Guangde County Anhui Province two places, adopt shuttle breeding, Nan Fanbei educates, and synchronously carries out sterile line breeding to F8 generation, selection pressure under increasing natural conditions, carry out manually being measured of a small amount of combination, a small amount of production of core seeds, the packaged combination isolation production of hybrid seeds; Male sterile line reaches F10, the wide 18S of called after from generation to generation.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954545A (en) * | 2017-03-02 | 2017-07-18 | 安徽金培因科技有限公司 | A kind of is the breeding method of line with genic sterile indica hybrid rice for light temperature sensitive two |
| CN108834878A (en) * | 2018-07-17 | 2018-11-20 | 安徽新安种业有限公司 | The breeding method of excellent Indica TGMS line rice |
| CN110050685A (en) * | 2019-04-09 | 2019-07-26 | 武汉弘耕种业有限公司 | A kind of seed choosing method of photo-thermo sensitive male nuclear sterile rice line |
-
2015
- 2015-02-05 CN CN201510061334.4A patent/CN104663424A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 宁会琴: "籼型光温敏两用核不育系广18S选育报告", 《现代农业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954545A (en) * | 2017-03-02 | 2017-07-18 | 安徽金培因科技有限公司 | A kind of is the breeding method of line with genic sterile indica hybrid rice for light temperature sensitive two |
| CN108834878A (en) * | 2018-07-17 | 2018-11-20 | 安徽新安种业有限公司 | The breeding method of excellent Indica TGMS line rice |
| CN110050685A (en) * | 2019-04-09 | 2019-07-26 | 武汉弘耕种业有限公司 | A kind of seed choosing method of photo-thermo sensitive male nuclear sterile rice line |
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Application publication date: 20150603 |