CN104855277A - Breeding method for bitter gourds with high yield - Google Patents

Breeding method for bitter gourds with high yield Download PDF

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Publication number
CN104855277A
CN104855277A CN201510322741.6A CN201510322741A CN104855277A CN 104855277 A CN104855277 A CN 104855277A CN 201510322741 A CN201510322741 A CN 201510322741A CN 104855277 A CN104855277 A CN 104855277A
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Prior art keywords
balsam pear
inbred line
breeding
male parent
male
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CN201510322741.6A
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Chinese (zh)
Inventor
谢河山
任淑梅
宫庆友
沈汉国
黄聪灵
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Modern Agricultural Development Center Zhuhai City
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Modern Agricultural Development Center Zhuhai City
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Priority to CN201510322741.6A priority Critical patent/CN104855277A/en
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Abstract

The invention aims at providing a breeding method for premature bitter gourds with high yield. The method comprises the steps of (1) breeding a female parent selfing line, namely carrying out six generations of selfing on the selected Zhuhai local bitter gourd variety with good agronomic traits, separating and purifying a good female parent selfing line, and marking the female parent selfing line as NK11; (2) breeding a male parent selfing line, namely carrying out seven generations of selfing on the selected bitter gourd variety in Penang in Malaysia, separating and purifying a good male parent selfing line, and marking the male parent selfing line as ZK10; and carrying out hybridization on female parent sefling line NK11 and male parent selfing line ZK10, thus obtaining good bitter gourd hybrids. The hybrid is named as Lvzhu II. The breeding method can be applied to the field of agricultural breeding.

Description

A kind of breeding method of high yield balsam pear
Technical field
The present invention relates to a kind of plant breeding method, particularly relate to a kind of breeding method of high yield balsam pear.
Background technology
Balsam pear (Momordica charantia L.), also known as balsam pear, balsam pear etc., the annual trailing plant of Curcurbitaceae Momordica, because its fruit surface has peculiar knurl wrinkle, containing charantin in fruit, has a kind of special bitter taste and gains the name, mainly edible with tender fruit.In China, main plantation Area distribution in Guangdong and Guangxi Provinces, Hainan, Fujian, Taiwan, Sichuan, Hunan, the southern provinces and regions such as Jiangxi.
Balsam pear is the vegetable crop that a kind of area under cultivation is larger, nutrition and relieving summer-heat function contained by it, and balsam pear is subject to general popular welcome.The annual area under cultivation in South China is more than 1,000,000 mu, Varieties of Balsam Pear in current production late ripe, low yield, disease resistance and commodity are poor, also some early-maturing varieties are had, but production application effect is not ideal enough, main cause is the contradiction that these kinds cannot solve between precocity and high yield, high-quality, resistance.Therefore, in the urgent need to precocity, high yield, high-quality, disease-resistant New variety of balsam pear.
Summary of the invention
Technical problem to be solved by this invention overcomes the deficiencies in the prior art, aims to provide a kind of high yield balsam pear breeding method.The New variety of balsam pear that the method can cultivate precocity, yielding ability is good, disease resistance is strong, wide adaptability, fruit marketability are good.
The technical solution adopted in the present invention is, the method comprises the following steps:
(1) the maternal inbred line of seed selection: select the local Varieties of Balsam Pear in the excellent Zhuhai of economical character to carry out the selfing of six generations, be separated and excellent maternal inbred line of purifying out, be labeled as NK11;
(2) seed selection male parent inbred line: select to carry out the selfing of seven generations from Malay Penang Varieties of Balsam Pear, is separated and excellent male parent inbred line of purifying out, is labeled as ZK10;
(3) maternal inbred line NK11 and male parent inbred line ZK10 is hybridized, obtain showing excellent balsam pear crossbreed.This balsam pear crossbreed is named as green pearl No. 2.
Further, the selfing process in described step (1) and described step (2) is: in male flower and the female flower bagging of giving homophyletic noon before that day respectively of pollination, within second day, selects the pollen of homophyletic male flower to pollinate to the female flower of homophyletic, bagging immediately after pollination.
Further, the process of described pollination is: get open male flower on the same day, remove petal, smeared gently by the pollen of homophyletic stamen on the column cap of gynoecium, pollination time with the morning 9 ~ 10 time be advisable.
Further, described female parent is the precocious strain of main climing many fruit type high-quality, in growth potential and point principal stresses, female stronger, fruit conico-acuminate, shoulder is flat, and fruit shape is rectified attractive in appearance, and pulp is thick, fruit look light green, and after 6 generations were selected, each proterties of inbred line is stablized, and names as NK11.
Further, in described step (2), male parent breeding goal for bear fruit many, bear fruit joint position low, the excellent melon of excellent strain that melon look pale green, uniformity, bar knurl are straight and dark, disease resistance is good, single melon that focal selection weight is larger, improves single melon and weighs and varietal resistance, annual spring and autumn carry out both parties in a lawsuit's bagging self cross and selection, after 7 generations were selected, each proterties of inbred line is stablized, and names as ZK10.
Further, in described step (3), carrying out the process that maternal inbred line NK11 and male parent inbred line ZK10 carries out hybridizing is: the male parent male flower that second day can bloom noon before that day by pollination and maternal female flower bagging respectively, during that morning that blooms pollination, male parent male flower is taken, pollinate to maternal female flower, give the female flower bagging after pollination again, and list on anthocaulus as mark, time ripe, pluck markd kind of melon.
The invention has the beneficial effects as follows: the step that breeding method of the present invention comprises is: the maternal inbred line of (1) seed selection: the local Varieties of Balsam Pear selecting economical character excellent is through the selfing of six generations, be separated and excellent maternal inbred line of purifying out, be labeled as NK11, (2) seed selection male parent inbred line: select from Malay Penang Varieties of Balsam Pear through the selfing of seven generations, be separated and excellent male parent inbred line of purifying out, be labeled as ZK10, (3) maternal inbred line NK11 and male parent inbred line ZK10 is hybridized, obtain showing excellent balsam pear Hybrid, called after " green pearl No. 2 ", after the Varieties of Balsam Pear plantation that the inventive method breeding obtains, its output is higher, and quality is higher, and disease-resistant rate is higher, is a kind of balsam pear breeding method be worthy to be popularized.
Embodiment
The present invention is further illustrated in a concrete fashion below.
The maternal NK11 of the present invention is selected from the Local Excellent kind of collecting local Cotton Varieties by Small Farming Households from lake, red flag town, Jinwan District, Zhuhai City East Village, and the Elite inbred that selects through the selfing Separation & Purification in 6 generations is the precocious strain of main climing many fruit type high-quality.This strain shows as in growth potential and point principal stresses, and comparatively early, 13rd ~ 15 joints start give birth to first female flower knot melon, and female comparatively strong, melon is many, and the climing result of master is good.Fruit conico-acuminate, shoulder is flat, and fruit shape is rectified attractive in appearance, and pulp is thick, and fruit look light green.Average melon long 24 ~ 26cm, the thick 6 ~ 7cm of melon, melon weighs 310 ~ 410g, and melon skin meat is medium; Mildew-resistance, downy mildew power are medium.
" ZK10 derives from Malay Penang balsam pear to male parent, and after 7 generations were selected, each proterties of inbred line is basicly stable, names as ZK10.Its characteristic is: plant strain growth gesture is vigorous, and point principal stresses is strong, and fruit type is thick, conico-acuminate, and bar knurl is slightly straight, and color of the leather is light green.In ripe, 16th ~ 18 save and give birth to the first female flower, are about 27 ~ 30cm, thick 7 ~ 8.5cm, the heavy 400-500 g of melon.High mildew-resistance, downy mildew.
Utilize NK11 as female parent, ZK10, as male parent, have selected growing way more prosperous, and disease resistance is strong, high temperature resistant, and the 1st female flower joint position is low, and result is many, the balsam pear hybrid new breed that output is high.Carried out varietal yield test to this kind, the combination participating in variety comparative test has green pearl No. 2 and other autogamy combination, and contrast kind is rich green balsam pear.3 repetitions are established in test, take randomized arrangement, plant the wide 1.8m of planting-line, long 15m, every plot area 27m 2, duplicate rows is planted, spacing in the rows 0.6m, every community field planting 60 strain, and peripheral hardware protection row, adopts man-like shelf mode to cultivate, and other management routinely oily body balsam pear planting type are carried out.Result of the test is as follows: the joint bit comparison of green pearl No. 2 balsam pears is low as shown in Table 1, and mean fruit weight is heavier than contrast, and average melon is long longer than contrast, and transverse diameter is thicker than contrast, and the contrast of meat thickness rate is thick; Early yield is 595.60 ㎏/667m as shown in Table 2 2, green balsam pear volume increase 10.95% richer in contrast; Gross yield is 2356.67 ㎏/667m 2, green balsam pear volume increase 12.31% richer in contrast.Gross yield and early yield all reach significant difference level.
the green pearl of table 1 No. 2 balsam pear major traits comparative test results
the green pearl of table 2 No. 2 quantity production of bitter melon comparative test results
Within 3rd year, be distributed to the multiple planting site in South China and carry out multispots trial, exhibits excellent, name as " green pearl No. 2 "." green pearl No. 2 " belongs to precocious Varieties of Balsam Pear, and growth potential is energetic, and branchiness is strong, easy setting.Main climing knot melon is main, main climing 12nd ~ 14 joint left and right raw first female flower.Commodity melon conico-acuminate, strip knurl, melon look light green, long 28 ~ 33cm, thick 6 ~ 8cm, the thick 1.0cm of meat.The heavy 500-600 gram of single melon, melon conico-acuminate, bar warty, skin is emerald green glossy, best in quality.
" green pearl No. 2 " cultivation management technology main points
South China In Spring was sowed at the beginning of 2 ~ 3 months, and ten days in summer July in autumn was sowed to mid or late August.Spring, sowing was to beginning receipts 65 ~ 75 days, and planted in fall was to beginning receipts 50 ~ 55 days.Sow last as with 50-55 DEG C of water seed soaking, continue seed soaking 4 ~ 6 hours after cooling, make its imbibition, with stratification, be seeded in nutritive cube after sprouting, water permeable before broadcasting, general table soil does not show money or valuables one carries unintentionally and does not water.Balsam pear requires strictly over the ground, and selection soil layer is deep, fertile, irrigation and drainage are local easily.Avoid continuous cropping, previous crops is preferably paddy rice or the plantation of non-melon crop field.Balsam pear is long for breeding time, and fertilizer requirement is large, therefore needs heavy dressing base manure, to meet balsam pear whole vegetative period to the demand of nutrient.Whole high furrow, spacing in the rows 70 ~ 90 cm, line-spacing 150 cm.Preferably adopt seed soaking, vernalization, nutritious bag (alms bowl) seedling method spring, when seedling grows to 3 ~ 4 true leaves, get final product field planting; Xia Qiuke adopts directly in seedling nursery on the ground, the field planting when seedling grows to 2-3 sheet true leaf.
Loose stool water extraction seedling is executed in field planting in time after delaying seedling, enter flowering and fruit bearing after date, remove side shoot in time, in order to avoid consume nutrient.Enter vigorous growth after date, every 20-30cm stays 1 lateral bine, and bondage is fixed.Later stage removes Lao Ye, sick leaf in time, ensures field ventilation and penetrating light, reduces disease and occurs.The disease of balsam pear mainly contains virus disease, powdery mildew, downy mildew and fusarium wilt, and insect pest mainly contains melon trypetid, pathogenicity test etc.Accomplish early to control anti-morning, rely mainly on prevention." green pearl No. 2 " belongs to large fruit Varieties of Balsam Pear.In order to be conducive to the growth of follow-up melon, generally after spending 15 ~ 18 days, gathering during single fruit weight 450 ~ 550 grams and being advisable.
The present invention can be applicable to agricultural breeding field.

Claims (6)

1. a breeding method for high yield balsam pear, is characterized in that, the method comprises the following steps:
(1) the maternal inbred line of seed selection: select the local Varieties of Balsam Pear in the excellent Zhuhai of economical character carry out six generation selfed breeding, be separated and excellent maternal inbred line of purifying out, be labeled as NK11;
(2) seed selection male parent inbred line: select from Malay Penang Varieties of Balsam Pear carry out seven generation selfed breeding, be separated and excellent male parent inbred line of purifying out, be labeled as ZK10;
(3) maternal inbred line NK11 and male parent inbred line ZK10 is carried out selection cross, obtain showing excellent balsam pear crossbreed, this crossbreed is named as green pearl No. 2.
2. the breeding method of a kind of high yield balsam pear according to claim 1, it is characterized in that, selfing process in described step (1) and described step (2) is: in male flower and the female flower bagging of giving homophyletic noon before that day respectively of pollination, the pollen of homophyletic male flower within second day, is selected to pollinate to the female flower of homophyletic, bagging immediately after pollination.
3. the breeding method of a kind of high yield balsam pear according to claim 2, it is characterized in that, the process of described pollination is: get the male flower opened the same day, remove petal, the pollen of homophyletic stamen is smeared gently on the column cap of gynoecium, pollination time with the morning 9 ~ 10 time be advisable.
4. the breeding method of a kind of high yield balsam pear according to claim 1, it is characterized in that: described female parent is the precocious strain of main climing many fruit type high-quality, in growth potential and point principal stresses, female stronger, fruit conico-acuminate, shoulder is flat, and fruit shape is rectified attractive in appearance, and pulp is thick, fruit look light green, after 6 generations were selected, each proterties of inbred line is stablized, and names as NK11.
5. the breeding method of a kind of high yield balsam pear according to claim 1, it is characterized in that, in described step (2), male parent breeding goal for bear fruit many, joint position of bearing fruit is low, the excellent melon of excellent strain that melon look pale green, uniformity, bar knurl are straight and dark, disease resistance is good, single melon that focal selection weight is larger, improve single melon to weigh and varietal resistance, annual spring and autumn carry out both parties in a lawsuit's bagging self cross and selection, after 7 generations were selected, each proterties of inbred line is stablized, and names as ZK10.
6. the breeding method of a kind of high yield balsam pear according to claim 1, it is characterized in that, in described step (3), carrying out the process that maternal inbred line NK11 and male parent inbred line ZK10 carries out hybridizing is: the male parent male flower that second day can bloom noon before that day by pollination and maternal female flower bagging respectively, during that morning that blooms pollination, male parent male flower is taken, pollinate to maternal female flower, then give the female flower bagging after pollination, and list on anthocaulus as mark, pluck markd kind of melon time ripe.
CN201510322741.6A 2015-06-14 2015-06-14 Breeding method for bitter gourds with high yield Pending CN104855277A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106106131A (en) * 2016-06-30 2016-11-16 漳州市农业科学研究所 A kind of Fructus Momordicae charantiae hybridization partition method
CN106508352A (en) * 2016-10-31 2017-03-22 龙门县顺喜来生态农业发展有限公司 Green production method of bitter gourd bagging
CN108849480A (en) * 2018-07-24 2018-11-23 福建农林大学 A kind of Varieties of Balsam Pear breeding method of the long spindle anti-blight of the alternate tumor of item
CN108848989A (en) * 2018-07-16 2018-11-23 贵州省果树科学研究所 A kind of method for culturing seedlings of complete female balsam pear
CN110235709A (en) * 2019-06-05 2019-09-17 澧县绿之源生态农业科技发展有限公司 A kind of cultural method of balsam pear breeding time ahead of time
CN114451290A (en) * 2022-02-12 2022-05-10 厦门农运来农业技术服务有限公司 Cultivation method of white oil bitter gourds

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106106131A (en) * 2016-06-30 2016-11-16 漳州市农业科学研究所 A kind of Fructus Momordicae charantiae hybridization partition method
CN106508352A (en) * 2016-10-31 2017-03-22 龙门县顺喜来生态农业发展有限公司 Green production method of bitter gourd bagging
CN108848989A (en) * 2018-07-16 2018-11-23 贵州省果树科学研究所 A kind of method for culturing seedlings of complete female balsam pear
CN108849480A (en) * 2018-07-24 2018-11-23 福建农林大学 A kind of Varieties of Balsam Pear breeding method of the long spindle anti-blight of the alternate tumor of item
CN110235709A (en) * 2019-06-05 2019-09-17 澧县绿之源生态农业科技发展有限公司 A kind of cultural method of balsam pear breeding time ahead of time
CN114451290A (en) * 2022-02-12 2022-05-10 厦门农运来农业技术服务有限公司 Cultivation method of white oil bitter gourds

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Application publication date: 20150826