CN110301348A - A kind of selection of purple loose type broccoli - Google Patents
A kind of selection of purple loose type broccoli Download PDFInfo
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- CN110301348A CN110301348A CN201910740634.3A CN201910740634A CN110301348A CN 110301348 A CN110301348 A CN 110301348A CN 201910740634 A CN201910740634 A CN 201910740634A CN 110301348 A CN110301348 A CN 110301348A
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- purple
- broccoli
- loose type
- selection
- loose
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a kind of selections of purple loose type broccoli, this method is maternal with the purple broccoli cytoplasmic male sterile line mostly obtained for backcross transformation, as male parent, hybridization obtains purple loose type broccoli characteristic new varieties for the local purple loose type broccoli DH system obtained using microspores culture.The method of the present invention selected bouquet purple is loose, bennet it is long it is green, rich in anthocyanin, crisp in taste, and new type broccoli first-filial generation new varieties that comprehensive resistance is excellent, yield is high.
Description
Technical field
The invention belongs to field of plant variety breeding technology, and in particular to a kind of breeding side of novel purple loose type broccoli
Method.
Background technique
Broccoli (Brassica oleracea L.var.italica) also known as broccoli, broccoli etc., are Cruciferae
Using green bouquet as the mutation of product in Brassica genus brassica specie.Broccoli originates from Mediterranean country, about 19 th century laters
Incoming China.Broccoli is full of nutrition and has healthcare function, vitamin rich in, minerals, be good blood vessel and
Enteron aisle cleaning agent；On the other hand, it is also rich in anti-cancer active matter glucosinolate, has significant anti-cancer, anticancer function.In recent years
Come, broccoli is becoming increasingly popular in China, and cultivated area is continuously increased, and becomes the maximum country of cultivated area in the world.
In China's broccoli breeding work starting evening, the germ plasm resource of grasp is few, and breeding technique falls behind, and causes to be bred as new varieties
The market competitiveness it is not strong.Currently, the seed of 90% or more the broccoli kind of the main cultivation in China come from import, market there is an urgent need to
Broccoli new varieties with independent intellectual property rights；On the other hand, the broccoli kind of domestic cultivation and sale is nearly all green
Dyeing defect ball, bouquet consolidation and low output, coarse mouthfeel are stiff.As the improvement of people's living standards, to vegetable nutrient and quality
Attention degree be increasingly enhanced, domestic market meets people to kind there is an urgent need to not only be fond of eating but also good-looking broccoli new varieties
The diversified demand of type.
To meet the market requirement, the application by the introduction of Broccoli Resources resource, microspore culture it is quick pure
Change, using cytoplasmic male sterile line approach, has formulated out with independent intellectual property rights, had both high yield, disease-resistant and high-quality
The loose type broccoli new varieties of purple, have a vast market application potential.
Summary of the invention
The purpose of the present invention is select purple loose type broccoli characteristic new varieties with independent intellectual property rights.It utilizes
Microspore culture is quickly obtained homozygous breeding parent, and combination cell matter male sterility approach has selected bouquet purple pine
It dissipates, is rich in anthocyanin, is crisp in taste, and the characteristic broccoli first-filial generation new varieties that comprehensive resistance is excellent, yield is high.
In order to achieve the above technical purposes, the present invention is realized especially by following technical scheme:
Male parent is in the local purple loose type broccoli DH system that the present invention is obtained using microspores culture, mostly for backcross transformation
Obtained purple broccoli cytoplasmic male sterile line is female parent, and the purple loose type broccoli that hybridization obtains a kind of new type is special
Color new varieties.
A kind of selection of purple loose type broccoli, specifically includes the following steps:
1) it is female parent with the broccoli kind in ogura cytoplasmic male sterility source, using purple broccoli as male parent, is turned
Educate a generation；
2) transformation generation plant selects and identifies through Agronomic characteristic, filters out excellent sterile single plant, carries out backcrossing and turns
It educates；
3) after 6 generation of continuous backcross, the cytoplasmic male sterile line of inheritance stability is obtained；
4) from local purple loose type broccoli, excellent strain is selected to carry out microspores culture, obtains homozygous double single times
Body (DH) plant filters out excellent dihaploid (doubled haploid, DH) system；
5) cytoplasmic male sterile line of the inheritance stability obtained with transformation is female parent, to filter out excellent DH system as father
This, preparing hybrid combination obtains first-filial generation kind；
6) first-filial generation kind compares by field planting, combination, selects bouquet elasticity and the discrepant new product of purple
Kind.
Further, the purple broccoli DH system is denoted as DH-BC-8.
Further, the local purple loose type broccoli for microspores culture being carried out in step (4) is denoted as SG1614.
Further, the DH system has characteristics that bouquet purple, loose, bennet length, green, crisp in taste, flower
Powder is normal.
The invention has the benefit that
1) purple broccoli has relatively big difference compared to common broccoli.Bud is compared with thin, color is dark purple；Bouquet is loose；Bennet
It is long, green, tender and crisp；Bouquet diameter is big, yield is high；Plant is big, and comprehensive resistance is excellent.Increase the diversity of broccoli type；
2) compared with common conventional green broccoli, which shows different nutrition and mouthfeel.Tool
There is the anthocyanidin content of healthcare function to dramatically increase, mouthfeel is crisp, tender, is suitable for eating it raw；
It 3) is DH system due to keeping system and parent, the purple loose type broccoli first cross kind of acquisition is neat, consistent,
The bouquet listing phase more concentrates, and is suitble to unified mechanized harvesting；
4) the purple broccoli obtained is formulated by cytoplasmic male sterile line, can carry out cenospecies with honeybee pollination
With numerous system of original silkworm egg, breeding cost is substantially reduced.
Detailed description of the invention
Fig. 1 is the flow chart of selection of the present invention.
Specific embodiment
Below in conjunction with specific embodiment of the present invention, technical solution of the present invention is clearly and completely described, is shown
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
As shown in Figure 1, present embodiments providing a kind of selection of purple loose type broccoli, following step is specifically included
It is rapid:
1) blue and white local kind of the germ plasm resource of 18 parts of characteristics is introduced from domestic and international within 2008, through Observation on Agronomic Characters and identification,
It was found that more parts of broccoli materials, show that leaf color is shallow, plant shape is big, bouquet is purple, growing way is strong, it is high to sit ball respectively, spherical surface presents different
Purple and form, some materials show bouquet rounding, loose, the different characteristics such as tender and crisp.Current year selection fine individual plant (strain) into
Row microspores culture and selfing purification work.
2) multiple purple materials carry out microspores culture, 58 DH systems are obtained altogether, by continuous 2 years Observation on Agronomic Characters
And identification, it is screened out from it the excellent DH strain (DH-BC-8, DH-SG614) of Comprehensive Traits.
3) with bud not chrysanthemum, petal be normally unfolded, the ogura cytoplasmic male sterility source of nectary normal development it is blue and white
Vegetable kind is female parent, with purple broccoli DH system (DH-BC-8) for male parent, carries out bud pollination selfing, obtains a transformation generation
(F1)。
4) transformation generation plant selects and identifies through Agronomic characteristic, filters out excellent sterile single plant, is with DH-BC-8
Recurrent parent carries out mostly for backcross transformation.After 6 generation of continuous backcross (BC6), the cytoplasmic male sterile line of inheritance stability is obtained
(DH-BC-8A)。
5) from local purple loose type broccoli, excellent strain (SG1614) is selected to carry out microspores culture, from obtaining
DH homozygous plants in filter out that bouquet purple, loose, stalk is long, crisp in taste, the normal excellent DH system (DH- of pollen
SG1614)。
6) the purple cytoplasmic male sterile line (DH-BC-8A) of the inheritance stability obtained with transformation is female parent, with DH system
It (DH-SG1614) is male parent, preparing hybrid combination obtains first cross kind.First cross kind compares by field planting, combination
And demonstration plant, each combination is comprehensive to show excellent, selects several bouquet elasticities and the slightly discrepant new product of purple
Kind.One of combination shows as yield height, and bouquet is dark purple, and bouquet is more loose, and bennet is green, long, and tender and crisp, comprehensive resistance is excellent
It is good, it is named as ' purple pine No. 1 '.
Embodiment 2
' purple pine No. 1 ' is bud purple, the loose broccoli cenospecies of bouquet, and late-maturing, autumn sowing is from field planting to 100 days left sides of harvest
It is right；Plant growing way is strong, and half is upright, and plant shape is big, the purple green of siphonal lobe；Bouquet rounding is loose, and the thin equal, purple of bud, bennet is green, long,
Single ball weight 1.5kg or so, the every 667m of Transplanting Density2It is 2000 plants or so.
The broccoli new type that the present invention cultivates, it is common that most outstanding feature, which is that the content of anthocyanidin is higher, in quality
10 times or more of green broccoli, in addition total sugar content is slightly higher than the control, while content of cellulose is than compareing slightly lower, other nutrition
Ingredient is suitable with control.
3 production of hybrid seeds of embodiment
Parent's breeding: breeding is isolated using greenhouse.Male parent selfing is not affine, needs artificial flower bud phase cabage-type rape.Female parent is protected
Holding is that selfing is not affine, needs artificial flower bud phase cabage-type rape；Maternal sterile line needs artificial flower bud phase stripping flower bud, keeps system with female parent
Back pollinating is collected seed.
First-filial generation system can take greenhouse and open country producing method for seed.First-filial generation is if you need to a large amount of production of hybrid seeds, it is proposed that using outdoor
The cold bed production of hybrid seeds.Maternal 3 rows are taken in the greenhouse production of hybrid seeds, and the implantation methods of 2 row of male parent, male parent was than maternal early sowing 15 days.
1) seed breeding base is strictly isolated: outdoor seed breeding base generally uses space to be isolated, i.e. within production of hybrid seeds field circumference 2km
The cabbage vegetable easily hybridized with broccoli cannot be planted.
2) adjustment date of seeding makes father, maternal flower synchronization: maternal sterile line breeding time and male parent are 15 days short, in cross breeding seed
It is needed in the process according to local climate, by adjusting sowing time, rich water regulates and controls and cut the florescence phase that bouquet mode ensures father, female parent
It meets, to improve seed collecting amount.
3) appropriate planting proportion: father, the maternal ratio according to 1:2 are planted, i.e. two furrow are maternal, centre one furrow male parent of folder.
4) field management: in In Hangzhou Region of Zhe Jiang Province, male parent is generally sowed in late August, and maternal infertility lies in early September sowing,
Seedling stage about 30 days i.e. 1 heart stage of 4 leaf of seedling, transplants vinyl house, and seeding row spacing is 45 × 55 centimetres.Second year mid or late March is bloomed,
Discharge bumblebee supple-mentary pollination.Moisture and fertilizer are controlled before blooming, and pay attention to pulling out for hybrid strain.When blooming, pay attention to rich water and
When supplement, reinforce the prevention and treatment of pest and disease damage.In Southwest China and the Northwest, male parent is generally sowed in early August, and female parent is in August
Last ten-days period sowing, seedling stage about 30 days i.e. 1 heart stage of 4 leaf of seedling, transplant outdoor base, and seeding row spacing is 45 × 55 centimetres.In second year 1 month
Ten days blooms, and discharges bumblebee supple-mentary pollination.Moisture and fertilizer are controlled before blooming, and pay attention to pulling out for hybrid strain.When blooming, fertilizer is paid attention to
Water is replenished in time, and reinforces the prevention and treatment of pest and disease damage.
5) harvest seed: the whole florescence pulls out male parent, and the seed harvested from maternal sterile line is first-filial generation kind.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understand without departing from the principles and spirit of the present invention can to these examples carry out it is a variety of variation, modification, replacement and
Modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of selection of purple loose type broccoli, which comprises the following steps:
1) it is female parent with the broccoli kind in ogura cytoplasmic male sterility source, using purple broccoli as male parent, obtains transformation one
Generation；
2) transformation generation plant selects and identifies through Agronomic characteristic, filters out excellent sterile single plant, carries out backcross transformation；
3) using purple broccoli as recurrent parent, after 6 generation of continuous backcross, the purple broccoli cytoplasmatic male of inheritance stability is obtained
Sterile line；
4) from local purple loose type broccoli, excellent strain is selected to carry out microspores culture, obtains homozygous plants, filters out
Excellent Doubled haploid line；
5) the purple broccoli cytoplasmic male sterile line of the inheritance stability obtained with transformation is female parent, to filter out excellent purple
Loose type broccoli DH plant is male parent, and preparing hybrid combination obtains first-filial generation kind；
6) first-filial generation kind compares by field planting, combination, selects bouquet elasticity and the discrepant new varieties of purple.
2. a kind of selection of purple loose type broccoli according to claim 1, which is characterized in that in step (1)
The purple broccoli DH system is denoted as DH-BC-8.
3. a kind of selection of purple loose type broccoli according to claim 1, which is characterized in that in step (4)
The local purple loose type broccoli and DH system for carrying out microspores culture are denoted as SG1614 and DH-SG1614 respectively.
4. a kind of selection of purple loose type broccoli according to claim 1, which is characterized in that the DH system
Have characteristics that bouquet purple, loose, spherical surface is flat, and bennet is long, green, and crisp in taste, pollen is normal.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111528100A (en) * | 2020-06-16 | 2020-08-14 | 上海市农业科学院 | Method for obtaining distant hybridization progeny of broccoli and brassica napus |
| CN111837934A (en) * | 2020-07-01 | 2020-10-30 | 浙江省农业科学院 | Breeding method of broccoli ball and seed variety with high glucoraphanin content |
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| CN104137769A (en) * | 2014-07-26 | 2014-11-12 | 西北农林科技大学 | Method for selectively breeding new high-quality disease-resistant variety of cabbage through DH lines |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111528100A (en) * | 2020-06-16 | 2020-08-14 | 上海市农业科学院 | Method for obtaining distant hybridization progeny of broccoli and brassica napus |
| CN111837934A (en) * | 2020-07-01 | 2020-10-30 | 浙江省农业科学院 | Breeding method of broccoli ball and seed variety with high glucoraphanin content |
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