CN104137769A - Method for selectively breeding new high-quality disease-resistant variety of cabbage through DH lines - Google Patents

Abstract

The invention discloses a method for selectively breeding a new high-quality disease-resistant variety of cabbage through DH lines. The method comprises the following steps that cabbage cytoplasmic male sterile lines CMS04G632-8-6-3 are selected as a female parent; the DH lines DH060Y03-35 are selected as a male parent; the female parent and the male parent are crossbred to form a CMS04G632-8-6-3xDH060Y03-35 combination which is named as the variety Qin Gan 1265. Through varietal yield tests, variety regional tests and production demonstration in Yangling, Xi'an, Baoji, Taibai and other main vegetable cultivation areas in Shaanxi Province, it shows that the variety is remarkable in heterosis, excellent in comprehensive economic character, outstanding in yielding ability, high in adaptability and stable in yield increase; compared with the cabbage variety Green Ball 66, the yield increase rate of the new variety ranges from 10.4 percent-12.4 percent.

Description

It is the method for the disease-resistant new quality variety of seed selection that a kind of wild cabbage utilizes DH

Technical field

The invention belongs to Crop Genetics and Breeding section in agricultural science and technology, be specifically related to the selection of the disease-resistant new quality variety of a kind of wild cabbage.

Background technology

Wild cabbage is one of China's Main Vegetable Species Suitable For Culture, and its breed breeding research Eleventh Five-Year Plan is put into national supporting plan project, and country determines that the breeding objective of wild cabbage medium variety is disease-resistant high grade producing.Virus disease (Virus disease) and black rot (Black rot disease, be called for short Br) be the Major Diseases in producing, wherein the main toxogen of virus disease is overgrown with weeds blue and white mosaic virus (Turnip mosaic Virus is called for short TuMV); Cabbage head miniaturization and high nutrition, the food value of leaf is crisp sweet, bag ball consolidation, the short and less consumer demand that becomes again of raw fiber of newel.Wild cabbage hybrid vigour is obvious, screen good inbred line and utilize hybrid vigour to become one of effective way of cultivating multiple target new varieties, in the anti-TuMV of seed selection, Br disease and high-quality, ripe parent is targetedly, is the method that cultivation first-filial generation realizes the disease-resistant high grade producing breeding objective of wild cabbage.

Summary of the invention

For above-mentioned problems of the prior art and defect, the object of the present invention is to provide a kind of wild cabbage to utilize DH is the method for the disease-resistant new quality variety of seed selection, the head cabbage varieties being bred as through the method is through varietal yield test, regional experiment of variety and production demonstration, performance hybrid vigour is remarkable, integral economic trait is good, yielding ability is outstanding, and varietal adaptation is strong, and volume increase advantage is stable.

To achieve these goals, the technical solution used in the present invention be a kind of wild cabbage to utilize DH be the method for the disease-resistant new quality variety of seed selection, specifically comprise the following steps:

1) seed selection of maternal germplasm materials CMS04G632-8-6-3

Maternal CMS04G632-8-6-3 cytoplasm male sterility line be wild cabbage half-blood " emerald " taking the preparation of Ogura kytoplasm wild cabbage male sterile line after maternal material and G632-8-6-3 hybridization between selfed lines, then through 5 generation backcross transformation form;

Maternal maintainer G632-8-6-3 inbred line is to obtain with inbred line HS85-136-16 and inbred line J05HR08-332-06 hybridization the original parent material that initial inbred line is selected, original parent material is carried out to the selfing seed selection of isozygotying, be bred as disease-resistant anti-source inbred line G632-8-6-3 through 5 generations selfing separation screening;

2) seed selection of male parent germplasm materials DH060Y03-35

Male parent DH060Y03-35 system is sweet 60 by High quality and diseases resistance first generation of hybrid kind Qin, the DH strain of cultivating 1 High quality and diseases resistance obtaining through Isolated microspore, then the DH obtaining through self propagated is DH060Y03-35;

3) breeding hybridized

Be that DH060Y03-35 is paternal hybrid preparation incubation, the called after Qin sweet 1265 taking wild cabbage cytoplasm male sterility line CMS04G632-8-6-3 as maternal and DH.

The Qin sweet 1265 is through varietal yield test, regional experiment of variety and production demonstration, vegetables cultivation area, Shaanxi Province Yang Ling, Xi'an, Baoji be too white etc., ground carries out varietal yield test and demonstration to this kind, hybrid vigour is remarkable, integral economic trait is good, yielding ability is outstanding, varietal adaptation is strong, and volume increase advantage is stable, than green ball 66 amounts of increase in production 10.4%～12.4% of contrast.

Sweet 1265 high antiviral disease and the black rot of the Qin, resistance to cracking ball, leaf-head commodity is good, and leaf-head outward appearance meets consumer's custom; Ball blade quality is crisp sweet, eats raw fragrant and sweet.

Brief description of the drawings

Fig. 1-1 is maternal wild cabbage cytoplasm male sterility line CMS04G632-8-6-3 and maintainer G632-8-6-3 seed selection figure.

Fig. 1-2 is maternal wild cabbage cytoplasm male sterility line CMS04G632-8-6-3 proterties figure.

Fig. 2-1 for male parent DH be the seed selection figure of DH060Y03-35.

Fig. 2-2 for male parent DH be DH060Y03-35 proterties figure.

Fig. 3-1 is the sweet 1265 breed breeding figure of the wild cabbage first generation of hybrid Qin.

Fig. 3-2 are the sweet 1265 varietal character figure of the wild cabbage first generation of hybrid Qin.

Embodiment

The specific embodiment providing by inventor below and concrete regional test are described in further detail beneficial effect of the present invention.

Embodiment 1

1. material source

The seed selection of 1.1 maternal germplasm materials CMS04G632-8-6-3

Maternal CMS04G632-8-6-3 cytoplasm male sterility line is that the wild cabbage half-blood " emerald " taking the preparation of Ogura kytoplasm wild cabbage male sterile line in 2004 is as maternal material and economic characters are good, coordinate force is high, high resistance multiple diseases G632-8-6-3 inbred line (maintainer) is hybridized, then formed through backcross transformation; Female parent is that proterties is isozygotied, and sterility is thorough, bloom normal, and nectary prosperity, staminody, gynoecium is normal, robust plant, not yellow of low temperature, without pollen grain, under normal temperature, all are normal except sterility for plant and floral organ; Ripe property mid-early maturity, field planting is arrived leaf-head ripe 65 days; Plant lotus throne leaf half is opened a business, and leaf look grayish green, and wax powder is more; Leaf-head discoid, newel 5.6cm, the degree of packing 0.55; High resistance TuMV, CMV, disease refers to≤1.34, black rot resistance, disease refers to 3.33; A little less than planting strain branchiness, spray growing way is strong, on average plants plant height degree 115.0cm, 30～35 days florescences.

Maternal maintainer G632-8-6-3 inbred line is to obtain by the high disease resistance inbred line J05HR08-332-06 hybridization of seed selection in of fine quality, the disease-resistant inbred line HS85-136-16 of the product of seed selection in the black leaf butch haircut of farm variety and balling consolidation kind (WGJ05) the original parent material that initial inbred line is selected in 1999; Give prominence to as inbred line breeding target according to disease resistance, quality and Comprehensive Traits, original parent material is carried out to the selfing seed selection of isozygotying.G632-8-6-3 is through 5 generations selfing separation screening, and character inheritance is stable, meets select target; When proterties is isozygotied, utilize wild cabbage TuMV, Br, CMV Resistance Identification method and wild cabbage main quality quality authentication method, carry out disease resistance and quality evaluation and screening; Screening in 2004 is bred as disease-resistant anti-source inbred line G632-8-6-3.Breeding technique route map (Fig. 1-1) and its Characters (Fig. 1-2) of maternal germplasm materials CMS04G632-8-6-3.

The seed selection of 1.2 male parent germplasm materials DH060Y03-35

Male parent DH060Y03-35 system selects its balling plant to an authorization wild cabbage first generation of hybrid kind Qin of High quality and diseases resistance country sweet 60 autumn in 2006, heels in and survives the winter; Field planting in spring field in 2007, and select its suitable bud to cultivate 1 the DH strain obtaining through Isolated microspore, the DH system of 1 High quality and diseases resistance obtaining by selfing and combining ability test, i.e. DH060Y03-35 system (Fig. 2-1, Fig. 2-2).Male parent is ripe in the ripe property of DH060Y03-35, and field planting was to leaf-head maturation about 70 days; Plant siphonal lobe is more, and strain state is closely upright, leaf look light green color, and in wax powder, leaf-head oblateness, leaf-head consolidation, the degree of packing 0.61; Best in quality, blade is crisp sweet, newel 5.5cm; High antiviral disease (TuMV disease refers to 0.3, CMV disease refer to 0.89) and black rot (disease refers to 2.89); In kind of strain branchiness, spray growing way is strong, flower compared with large, pollen amount is many, self-compatible, on average plants plant height 121.5cm, 30～35 days florescences.

1.3 breeding hybridized

Utilizing wild cabbage cytoplasm male sterility line CMS04G632-8-6-3 and DH is that DH060Y03-35 hybridization preparation is bred as, and the called after Qin is sweet 1265, the sweet 1265 breed breeding figure of Fig. 3-1 wild cabbage first generation of hybrid Qin.

1.3.1 the feature of the Qin sweet 1265:

Sweet 1265 (CMS04G632-8-6-3 × DH060Y03-35) of the Qin are ripe first generation of hybrid kind in autumn wild cabbage, and field planting was to results about 65 days; Plant plant type half is upright, blade celadon, and blade face is level and smooth, and wax powder is many; The outer stem of leave sheet short and thick, plant degree of development 46.2～48.8cm, 13～15 of the outer numbers of sheets, single ball weighs 1.35～1.45kg, the oblate type of leaf-head, the leaf-head degree of packing 0.58, it is high by 1 half that core length is less than ball, and side leaf is than 23.5%.High antiviral disease and black rot, resistance to cracking ball, warm tolerance is strong, and Fig. 3-2 are the sweet 1265 varietal character figure of the wild cabbage F1 Qin.

1.3.2 the variety and quality of the Qin sweet 1265:

Leaf-head commodity is good, and leaf-head outward appearance meets consumer's custom; Ball food value of leaf ground is tender and crisp, eats raw fragrant and sweetly, and flavor quality is good.

Its Major Nutrient analysis of components: raw fiber 0.53%, Vc39.86mg/100g, soluble sugar 9.10%, dry matter 8.35%.

1.3.3 suitable area and yield level

This kind is autumn wild cabbage medium variety, main suitable northern area cultivation early autumn/autumn and winter and southern autumn culture, 667m ²output average level 4603.4～5282.7kg.

1.3.4 culture technique main points

1) date of seeding: Shaanxi Province's autumn culture mid-June～early July overboard seeding and seedling raising, every 667m ²45～50 grams of seeding quantitys.

2) cultivating bed formula: flat furrow or half high bedding cultivation.

3) field planting: spray the field planting that sticks together of medicine insect-proof tape soil, plantation density line-spacing 55～60cm, spacing in the rows 40～45cm, every 667m while having 6～7 true leaves when seedling is long ²cultivation 2700～2800 strains.

4) field management:

1. pour water: when young plant field planting, temperature is higher, after field planting, pour water in time, steady seedling water is unsuitable excessive for the first time, too much; After being separated by 1～2 day, pour water again, in order to cooling, delay seedling, keep a full stand of seedings.Rosette stage to balling mid-term, filled with a water every 6～10 days, keep seeing and dryly see that moor is; Balling later stage irrigation period extends relatively, reduces or stop pouring water after balling-up.

2. fertilising: fertilising is made base fertilizer with barnyard manure or composite fertilizer, after field planting 7～10 days in conjunction with in chase after while restraining the growth of seedlings fertilely for the first time, divide lotus throne mid-term later, bag heart early stage and gradation in mid-term are topdressed, before plant envelope ridge, cave-applied fertilizer, envelope ridge heel water topdress, and 20～30 kilograms/667m of urea topdresses at every turn ²5 kilograms/667m of+potash fertilizer ²or 5000 kilograms/667m of night soil ², in the balling later stage, needn't topdress again.

3. pest control: select the insects such as physical method or low toxicity, public nuisance-free agricultural chemicals control cabbage caterpillar, aphid, ant, tomato moth.

Test example 1

Combination (F ₁) apolegamy: within 2009, select taking CMS04G632-8-6-3 cytoplasm male sterility line as maternal, DH selfing with 10 good, anti-TuMV of economic characters such as DH060Y03-35, Br, CMV is male parent, hybridization combo is researched and analysed coordinate force, and through 2009～2011 years, in Yang Ling, Xi'an, Baoji, the ground such as too white carried out varietal yield test and demonstration.Its result (table 1) can be found out, CMS04G632-8-6-3 × DH060Y03-35 combination (code name C19 × D06 (Qin sweet 1265)) hybrid vigour is remarkable, integral economic trait is good, disease resistance, quality and yielding ability are outstanding, meet breeding of new varieties target, field planting was to results about 65 days, best in quality, the food value of leaf is crisp sweet, and commodity is good, leaf-head oblateness, newel 0.56cm (being less than ball high by 1/2), the leaf-head degree of packing 0.58, receives 7 days evening without blastomere, average 667m ²output 4791.8kg, kind comparative result volume increase advantage is stable, than the green ball 66 (4335.2kg/667m of contrast ²) volume increase 10.5%; What this combination performance was the most outstanding is that disease resistance is strong, and the disease of TuMV, Br, CMV refers to that be 1.1,3.3,0.4 seedling stage, and field is 1.3,4.4, and phenotype reaches high resistance or disease-resistant; Comprehensive Traits comparison occupies first of combination comparative test and the Disease Resistance Identification of participating in the experiment of the same type for 3 years.

Table 1: 10 parts of F of wild cabbage ₁main target proterties comparative result (2009～2011 annual mean)

2. the first generation of hybrid " Qin sweet 1265 " kind innovative point

2.1 high resistances (HR) TuMV, Br and CMV disease.

Utilize wild cabbage Seedling Inoculation TuMV, Br and CMV Resistance Identification method, select 4 kinds of zones of different band TuMV toxogen separators, Br pathogen separation thing and CMV Shaanxi toxogens, to good F ₁" Qin sweet 1265 " kind is carried out Seedling Inoculation qualification; The field disease result of experimental investigation each point, carries out field resistance analysis.Result shows, check variety " green ball 66 " (CK) shows high resistance or disease-resistant, and seedling stage and field disease refer to change between 1.3～9.4; F ₁" Qin sweet 1265 " high disease resistance of Performance of cultivar, Seedling Inoculation refers to that to the disease of TuMV, CMV and Br average performance is respectively 1.1,0.4 and 1.3, field disease refers to virus disease 1.3, black rot 1.67; Its disease resistance is better than contrast, and disease refers to reduce by 0.54～7.73 (table 2).

2.2 best in quality, commodity good.

Through utilizing Cabbage Quality quality authentication method, the eating raw and the qualification performance of prepared food mouthfeel of the edible quality to leaf-head, F ₁the leaf-head food value of leaf of " Qin sweet 1265 " kind is tender and crisp, fragrant and sweet; Side leaf ratio, the degree of packing, newel measurement reach gold standard, and relatively check variety " green ball 66 " (CK) is helped leaf than reducing 2.6%, and the degree of packing increases 0.05g/cm ³, the short 0.05cm of newel.Show thus F ₁" Qin sweet 1265 " kind ball food value of leaf ground is crisp sweet, balling consolidation, and ball leaf edible part content is many, and quality is better than check variety (table 2).

Table 2: the sweet 1265 kind objective trait disease resistances of the Qin and quality qualification result

Note: HR: high disease-resistant, R: disease-resistant, MR: in anti-, S: susceptible, HS: high susceptible.

2.3 yielding abilities are good, strong adaptability.

New varieties combining ability test in 2009 filters out good combination CMS04G632-8-6-3 × DH060Y03-35 and combines (code name C19 × D06 (Qin sweet 1265)), through varietal yield test, regional experiment of variety and production demonstration (table 3, table 4), the ground hybrid vigours such as this kind is too white in Yang Ling, Xi'an, Baoji, Hanzhong are remarkable, integral economic trait is good, and yielding ability is outstanding; 667 ㎡ output average level 4603.4～5282.7kg, volume increase advantage is stable, than green ball 66 amounts of increase in production 10.4%～12.4% of contrast.Show to the plant characteristic Ge district pilot of this kind and demonstration the uniformity of height, plant degree of development 46.2～48.8cm, 13～15 of the outer numbers of sheets, single ball weighs 1.35～1.45kg, and field planting is to leaf-head results 65d left and right.

Table 3: sweet 1265 varietal yield tests of the Qin and the performance of production demonstration output

Table 4: sweet 1265 regional experiment of variety of the Qin and production demonstration yield result

3. conclusion

Sweet 1265 (CMS04G632-8-6-3 × DH060Y03-35) of the Qin are ripe first generation of hybrid kind in autumn wild cabbage, and field planting was to results about 65 days; Utilizing wild cabbage cytoplasm male sterility line CMS04G632-8-6-3 and DH is that DH060Y03-35 hybridization preparation is bred as.Plant plant type half is upright, blade celadon, and blade face is level and smooth, and wax powder is many; The outer stem of leave sheet short and thick, plant degree of development 46.2～48.8cm, 13～15 of the outer numbers of sheets, single ball weighs 1.35～1.45kg, the oblate type of leaf-head, the leaf-head degree of packing 0.58, it is high by 1/2 that core length is less than ball, and side leaf is than 23.5%.High antiviral disease and black rot, resistance to cracking ball.Leaf-head commodity is good, and leaf-head outward appearance meets consumer's custom; Ball blade quality is crisp sweet, eats raw fragrant and sweetly, and flavor quality is good.Varietal adaptation is strong, 667m ²output average level 4603.4～5282.7kg, variety trial result volume increase advantage is stable, than green ball 66 amounts of increase in production 10.4%～12.4% of contrast.

Claims (1)

1. to utilize DH be a method for the disease-resistant new quality variety of seed selection to wild cabbage, it is characterized in that, comprises the following steps:

1) seed selection of maternal germplasm materials CMS04G632-8-6-3

Maternal CMS04G632-8-6-3 cytoplasm male sterility line be taking with the wild cabbage half-blood " emerald " of Ogura kytoplasm wild cabbage male sterile line preparation after maternal material and wild cabbage G632-8-6-3 hybridization between selfed lines, then through 5 generation backcross transformation form;

Maternal maintainer G632-8-6-3 inbred line is to obtain with inbred line HS85-136-16 and high disease resistance inbred line J05HR08-332-06 hybridization the original parent material that initial inbred line is selected, original parent material is carried out to the selfing seed selection of isozygotying, be bred as disease-resistant anti-source inbred line G632-8-6-3 through 5 generations selfing separation screening;

2) seed selection of male parent germplasm materials DH060Y03-35

Male parent DH060Y03-35 system is sweet 60 by High quality and diseases resistance first generation of hybrid kind Qin, the DH strain of cultivating 1 High quality and diseases resistance obtaining through Isolated microspore, then the DH obtaining through self propagated is DH060Y03-35;

3) breeding hybridized

Seed selection is that DH060Y03-35 combines as paternal hybrid preparation is bred as CMS04G632-8-6-3 × DH060Y03-35 taking wild cabbage cytoplasm male sterility line CMS04G632-8-6-3 as maternal and DH, sweet 1265 kinds of the called after Qin.