CN104620969A - Rapid breeding method of Sargassum fusiforme - Google Patents
Rapid breeding method of Sargassum fusiforme Download PDFInfo
- Publication number
- CN104620969A CN104620969A CN201510075214.XA CN201510075214A CN104620969A CN 104620969 A CN104620969 A CN 104620969A CN 201510075214 A CN201510075214 A CN 201510075214A CN 104620969 A CN104620969 A CN 104620969A
- Authority
- CN
- China
- Prior art keywords
- female
- cultivation
- male
- seedling
- breeding method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a rapid breeding method of Sargassum fusiforme. The method includes the steps of selecting parents, performing isolation cultivation to single plants, promoting maturity, allowing fertilization, screening juvenile sporophytes, cultivating the juvenile sporophytes and the like. During isolation cultivation of the single plants, the weight ratio of the parents to cultivation water is 1:100; maturity promoting liquid is natural seawater added monopotassium phosphate, calcium chloride and sodium ethylene diamine tetracetate, under salinity of 27%. to 28%.; during fertilization, the ratio of females to males is 5:1. The method has the advantages that the technical defect of germplasm degeneration in the prior art is overcome, high-quality seedlings of Sargassum fusiforme can be obtained, plant dry weight of Sargassum fusiforme bred by the method is up to 247.7g, sac diameter is up to 4.1mm, and by the use of the method compared to the traditional methods, yield per mu of the plant dry weight is increased by 26.1%, the sac diameter is increased by 41.1%, and economic benefit is increased by 37.8%.
Description
Technical field
The present invention relates to a kind of sargassum fusifome breeding method, be specifically related to a kind of sargassum fusifome rapid breeding method, belong to sargassum fusifome Technique of Raising Seeedling field.
Background technology
Sargassum fusifome (Hizikia fusiforme (Harv) Okamura) is under the jurisdiction of Phaeophyta, dumpling made of glutinous rice flour guiding principle, Fucales, Sargassaceae, Hizikia, is the unique class of this genus.Individual large, the fast growth of sargassum fusifome frond, has the ecological effect of good cultivation, by gathering, just the carbon in sea area, nitrogen, phosphorus can be fixed with the form of former algae and be transported on the bank, and being processed as different product forms, remarkable in economical benefits.Research finds that the geographical population proterties of the sargassum fusifome kind of China's cultivation and the kind of Korea S exists notable difference, long 2 months of Korea S's sargassum fusifome growth cycle, generally ripe in July, sargassum fusifome finished bag particle is large and wall thickness, branches and leaves are sturdy, the soaked rear reconstitution rate of dry product is high, and the May in Wenzhou has gathered, vegetative period, length also made Korea S's sargassum fusifome fuller.Fishy smell is poor, mouthfeel is poor, brittleness is poor, eats and is more added with texture.
Sargassum fusifome is the economical alga of a kind of perennial male and female difference strain, and seedling needed for traditional cultivation derives from intertidal zone to gather wild Natural Population.Development in recent years rhizoid is aestivated the technology of regrowth, if publication number is the application for a patent for invention of CN1817111, discloses after sargassum fusifome vertical branch of gathering, retains rhizoid, cultivate in mariculture area, to autumn, and rhizoid vegetative seedling.Carry out indoors artificial recently and cultivate juvenile sporophyte, if notification number is the patent of invention of CN1326449, then disclose sargassum fusifome juvenile sporophyte under temperature, salinity, illumination, nutritional supplementation and flowing water such as to irritate at the qualifications, keep a full stand of seedings with the nursery of indoor culture pond, enter sea area to cultivate, obtain good result.Publication number CN1736164, name is called the patent of invention of " a kind of store method of sargassum fusifome sporophyte seedling ", also the store method of sargassum fusifome sporophyte seedling is disclosed, during cultivation, need low temperature (5 ~ 20 DEG C), and the stimulation by running water of cycle water and change water in time, can incubation rate be improved.
The type of rearing of rhizoid vegetative seedling and artificial culture raising seedling of young sporophyte has successfully solved sargassum fusifome seed and has carried out source problem; But, in same mariculture area, there is the multiple seed such as rhizoid vegetative seedling and artificial culture sporophyte seedling originate, often the seed cultivation in same source is in same marine site, result in the incidence of higher selfing, and such offspring continues to breed juvenile sporophyte, degenerate causing the kind matter of cultivation offspring, and seed rearing person does not consider the problem of kind, Stochastic choice plants vegetables, with the quantity of seedling for sole criterion; Equally, raiser also has no way of selecting, and causes the kind matter of sargassum fusifome to be degenerated comparatively serious.
Summary of the invention
Technical problem to be solved by this invention, that a kind of sargassum fusifome rapid breeding method is provided for the deficiencies in the prior art, the method can make up the technological deficiency of the kind matter degeneration existed in prior art, can obtain the high-quality sargassum fusifome seed of high-quality.
The present invention is achieved through the following technical solutions:
A kind of sargassum fusifome rapid breeding method, comprises the following steps:
(1), Juvenile stage: the sargassum fusifome selecting fast growth under high temperature, accumulation dry matter fast is as parent; Parent's branches and leaves are sturdy, internode is short, and air bag diameter is wall thickness greatly, and gum level is enriched, and color is dark;
(2), individual plant isolation is cultivated and accelerating: the parent chosen is screened male and female, and female, male individuality carries out unisexuality cultivation respectively, and namely female individuality is placed on a culture pond, and male individuality is at another culture pond; Female or male individuality is clipped in different Miao Shengzhong respectively, is suspended on respectively in the water body top layer of accelerating culture fluid and carries out accelerating cultivation; Cultivate to ripe, now there is mucus and projection in receptacle surface, cuts off ripe receptacle with scissors, continues to cultivate, obtain individuality ripe late;
(3), be fertilized: when receptacle is full in female or male individuality, carry out microscopy, the microscopy male that finds to dry in the shade has sperm to release and the receptacle of female individuals when there is opening, male and female individuality is placed in same water body, stirs water body, complete fertilization;
(4), juvenile sporophyte screening: after fertilization, fertilized egg is attached in female reproduction holder, and now female reproduction holder is obviously outstanding and color is darker; Fertilized egg is juvenile sporophyte after departing from receptacle, selects the juvenile sporophyte that the separation time is more late, with 150 object bolting silk screening juvenile sporophytes, discards the juvenile sporophyte that bolting silk spills; Again screen juvenile sporophyte with 150 mesh sieve thin,tough silk, the juvenile sporophyte selecting diameter larger is for subsequent use;
(5), the cultivation of juvenile sporophyte: be positioned over by the juvenile sporophyte of screening on the door curtain made of cloth of sargassum fusifome cultivation, indoor hydrostatic cultivates 12h, then indoor water-flowing to cultivate in pond nursery 10-15 days, be 0.1-0.5mm to juvenile sporophyte length; Move to sea area and carry out temporary culture of seedling, when seedling length is 1.0-2.5cm, growth selection faster seedling is seedling, seedling is clipped in Miao Shengshang and cultivates, and determines strain observation.
Preferably, the unisexuality described in step 2 is cultivated, and female or male sargassum fusifome parent is 1:100 with respective weight ratio of cultivating water body.
Preferably, the accelerating culture fluid described in step 2 is the nature seawater adding potassium dihydrogen phosphate, calcium chloride, sodium ethylene diamine tetracetate, and salinity is 25-30 ‰; Each concentration of component is: potassium dihydrogen phosphate 5ppm, calcium chloride 0.2ppm, sodium ethylene diamine tetracetate 0.5ppm.
Preferably, the cultivation in step 2 and accelerating condition are: inflation is cultivated, and cultivation temperature is 23-25 DEG C, and illumination is 3000-6000Lx.
Preferably, in step 3, female, male individual ratio is 5:1.
Preferably, the juvenile sporophyte selecting diameter larger described in step 4, its diameter >=0.1mm.
Preferably, the folder seedling described in step 5, the spacing of adjacent two seedling is 6cm.
Method of the present invention, technology path is clear and definite, simple to operate, easily obtains the excellent kind of sargassum fusifome; Compared with traditional breeding method mode, the sargassum fusifome per mu yield strain dry weight that the present invention obtains improves 26.71%, and air bag diameter increases 41.1%, and economic benefit has promoted 37.8%.
Embodiment
Below in conjunction with specific embodiment, sargassum fusifome rapid breeding method of the present invention is described in detail.
Embodiment 1:
A kind of sargassum fusifome rapid breeding method, comprises the following steps:
(1), Juvenile stage: the sargassum fusifome selecting fast growth under high temperature, accumulation dry matter fast is as parent; Parent's branches and leaves are sturdy, internode is short, and air bag diameter is wall thickness greatly, and gum level is enriched, and color is dark;
(2), individual plant isolation is cultivated and accelerating: the parent chosen is screened male and female, and female, male individuality carries out unisexuality cultivation respectively, and female or male sargassum fusifome parent is 1:100 with respective weight ratio of cultivating water body; Female or male individuality is clipped in different Miao Shengzhong respectively, is suspended on respectively in the water body top layer of accelerating culture fluid and carries out accelerating cultivation; Cultivate to ripe, cut off ripe receptacle with scissors, continue to cultivate, obtain individuality ripe late; Described accelerating culture fluid is the nature seawater adding potassium dihydrogen phosphate, calcium chloride, sodium ethylene diamine tetracetate, and salinity is 27-28 ‰; Each concentration of component is: potassium dihydrogen phosphate 5ppm, calcium chloride 0.2ppm, sodium ethylene diamine tetracetate 0.5ppm; Cultivation and accelerating condition are: inflation is cultivated, and cultivation temperature is 23-25 DEG C, and illumination is 4000-5000Lx;
(3), be fertilized: when receptacle is full in female or male individuality, carry out microscopy, the microscopy male that finds to dry in the shade has sperm to release and the receptacle of female individuals when there is opening, male and female individuality is placed in same water body, stirs water body, complete fertilization; Female, male individual ratio is 5:1;
(4), juvenile sporophyte screening: after fertilization, fertilized egg is attached in female reproduction holder, and fertilized egg is juvenile sporophyte after departing from receptacle, selects the juvenile sporophyte that the separation time is more late, with 150 object bolting silk screening juvenile sporophytes, discard the juvenile sporophyte that bolting silk spills; Again screen juvenile sporophyte with 150 mesh sieve thin,tough silk, the juvenile sporophyte selecting diameter larger is for subsequent use, its diameter >=0.1mm;
(5), the cultivation of juvenile sporophyte: be positioned over by the juvenile sporophyte of screening on the door curtain made of cloth of sargassum fusifome cultivation, indoor hydrostatic cultivates 12h, then indoor water-flowing to cultivate in pond nursery 12-13 days, be 0.2-0.3mm to juvenile sporophyte length; Move to Ni Dongping sea area, Dongtou County, Zhejiang Province and carry out temporary culture of seedling, when seedling length is 1.5-2.0cm, growth selection faster seedling is seedling, seedling is clipped in Miao Shengshang and cultivates, and the spacing of adjacent two seedling is 6cm, determines strain observation;
(6) set up contrast culture experiment: 2013-2014 years, the sargassum fusifome that breeding method of the present invention obtains and the sargassum fusifome that conventional method (control group) breeding obtains compare, and result is as shown in table 1:
As shown in Table 1, the sargassum fusifome that the inventive method breeding obtains, its strain dry weight reaches 247.7g, and the sargassum fusifome strain dry weight that conventional method obtains is 195.5g, and the present invention is compared with conventional method, and the per mu yield of strain dry weight improves 26.71%; The sargassum fusifome that the inventive method breeding obtains, its air bag diameter reaches 4.1mm, and the sargassum fusifome air bag diameter that conventional method breeding obtains is 2.9mm, and compared with prior art, air bag diameter increases 41.1% in the present invention; The present invention is compared with conventional art, and economic benefit has promoted 37.8%.
Above-mentioned example just for technical conceive of the present invention and technical characterstic are described, can not limit the scope of the invention with this.The equivalent transformation that all essence according to the present invention is done or modification, all should be encompassed within protection scope of the present invention.
Claims (7)
1. a sargassum fusifome rapid breeding method, is characterized in that, comprises the following steps:
(1), Juvenile stage: the sargassum fusifome selecting fast growth under high temperature, accumulation dry matter fast is as parent; Parent's branches and leaves are sturdy, internode is short, and air bag diameter is wall thickness greatly, and gum level is enriched, and color is dark;
(2), individual plant isolation is cultivated and accelerating: the parent chosen is screened male and female, and female, male individuality carries out unisexuality cultivation respectively; Female or male individuality is clipped in different Miao Shengzhong respectively, is suspended on respectively in the water body top layer of accelerating culture fluid and carries out accelerating cultivation; Cultivate to ripe, cut off ripe receptacle with scissors, continue to cultivate, obtain individuality ripe late;
(3), be fertilized: when receptacle is full in female or male individuality, carry out microscopy, the microscopy male that finds to dry in the shade has sperm to release and the receptacle of female individuals when there is opening, male and female individuality is placed in same water body, stirs water body, complete fertilization;
(4), juvenile sporophyte screening: fertilized egg is juvenile sporophyte after departing from receptacle, and select the juvenile sporophyte that the separation time is more late, screen juvenile sporophyte twice with 150 object bolting silks, the juvenile sporophyte selecting diameter larger is for subsequent use;
(5), the cultivation of juvenile sporophyte: be positioned over by the juvenile sporophyte of screening on the door curtain made of cloth of sargassum fusifome cultivation, indoor hydrostatic cultivates 12h, then indoor water-flowing to cultivate in pond nursery 10-15 days, be 0.1-0.5mm to juvenile sporophyte length; Move to sea area and carry out temporary culture of seedling, when seedling length is 1.0-2.5cm, growth selection faster seedling is seedling, seedling is clipped in Miao Shengshang and cultivates, and determines strain observation.
2. breeding method according to claim 1, is characterized in that, the unisexuality described in step 2 is cultivated, and female or male sargassum fusifome parent is 1:100 with respective weight ratio of cultivating water body.
3. breeding method according to claim 1, is characterized in that, the accelerating culture fluid described in step 2 is the nature seawater adding potassium dihydrogen phosphate, calcium chloride, sodium ethylene diamine tetracetate, and salinity is 25-30 ‰; Each concentration of component is: potassium dihydrogen phosphate 5ppm, calcium chloride 0.2ppm, sodium ethylene diamine tetracetate 0.5ppm.
4. breeding method according to claim 1, is characterized in that, the cultivation in step 2 and accelerating condition are: inflation is cultivated, and cultivation temperature is 23-25 DEG C, and illumination is 3000-6000Lx.
5. breeding method according to claim 1, is characterized in that, in step 3, female, male individual ratio is 5:1.
6. breeding method according to claim 1, is characterized in that, the juvenile sporophyte selecting diameter larger described in step 4, its diameter >=0.1mm.
7. breeding method according to claim 1, is characterized in that, the folder seedling described in step 5, and the spacing of adjacent two seedling is 6cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510075214.XA CN104620969B (en) | 2015-02-12 | 2015-02-12 | Rapid breeding method of Sargassum fusiforme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510075214.XA CN104620969B (en) | 2015-02-12 | 2015-02-12 | Rapid breeding method of Sargassum fusiforme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104620969A true CN104620969A (en) | 2015-05-20 |
| CN104620969B CN104620969B (en) | 2017-01-11 |
Family
ID=53200626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510075214.XA Active CN104620969B (en) | 2015-02-12 | 2015-02-12 | Rapid breeding method of Sargassum fusiforme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104620969B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075845A (en) * | 2015-08-24 | 2015-11-25 | 温州大学 | Selecting and breeding method for high-quality hizikia fusiformis |
| CN105104164A (en) * | 2015-08-20 | 2015-12-02 | 温州大学 | Quality breeding method for sargassum fusiforme |
| CN105532421A (en) * | 2015-12-16 | 2016-05-04 | 胡彩军 | Method for artificially producing sargassum fusiforme seedlings in freshwater |
| CN107155865A (en) * | 2017-07-10 | 2017-09-15 | 温州市洞头区水产科学技术研究所 | A kind of method for propagating sargassum fusifome artificially |
| CN110122312A (en) * | 2019-03-11 | 2019-08-16 | 华南理工大学 | A method of improving sargassum fusifome growth of seedling |
| CN110852647A (en) * | 2019-11-09 | 2020-02-28 | 温州大学 | Quality grade classification method for air bag outlet products of cultivated sargassum fusiforme |
| CN112806251A (en) * | 2021-02-26 | 2021-05-18 | 温州市洞头区海洋与渔业发展研究中心 | Unit mass evaluation and difference comparison method for cultivating juvenile sporophytes of sargassum fusiforme |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1726764A (en) * | 2004-07-30 | 2006-02-01 | 中国科学院海洋研究所 | The reproduction of brown alga Sargassum fusiforme (Harv.) Setch induces and utilizes the regulation and control fertilization to carry out the method that seedling is produced |
| CN1736164A (en) * | 2005-07-22 | 2006-02-22 | 宁波大学 | Method for preserving juvenile sporophyte of hijiki |
| CN1817111A (en) * | 2006-03-05 | 2006-08-16 | 孙庆海 | Yangxicai breeding technology |
| CN102224800A (en) * | 2011-04-29 | 2011-10-26 | 浙江省海洋水产养殖研究所 | Summering cultivation method for seedlings of Hizikia fusiform (Harv)Okamura juvenile sporophytes |
| CN104115738A (en) * | 2014-07-29 | 2014-10-29 | 广东海洋大学 | Seedling method for inducing synchronous maturity of Leizhou gulfweed in vitro receptacles |
-
2015
- 2015-02-12 CN CN201510075214.XA patent/CN104620969B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1726764A (en) * | 2004-07-30 | 2006-02-01 | 中国科学院海洋研究所 | The reproduction of brown alga Sargassum fusiforme (Harv.) Setch induces and utilizes the regulation and control fertilization to carry out the method that seedling is produced |
| CN1736164A (en) * | 2005-07-22 | 2006-02-22 | 宁波大学 | Method for preserving juvenile sporophyte of hijiki |
| CN1817111A (en) * | 2006-03-05 | 2006-08-16 | 孙庆海 | Yangxicai breeding technology |
| CN102224800A (en) * | 2011-04-29 | 2011-10-26 | 浙江省海洋水产养殖研究所 | Summering cultivation method for seedlings of Hizikia fusiform (Harv)Okamura juvenile sporophytes |
| CN104115738A (en) * | 2014-07-29 | 2014-10-29 | 广东海洋大学 | Seedling method for inducing synchronous maturity of Leizhou gulfweed in vitro receptacles |
Non-Patent Citations (2)
| Title |
|---|
| 骆其君: "羊栖菜幼孢子体苗海区培育的研究", 《海洋湖沼通报》 * |
| 骆其君等: "羊栖菜幼孢子体室内的培育", 《宁波大学学报》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104164A (en) * | 2015-08-20 | 2015-12-02 | 温州大学 | Quality breeding method for sargassum fusiforme |
| CN105075845A (en) * | 2015-08-24 | 2015-11-25 | 温州大学 | Selecting and breeding method for high-quality hizikia fusiformis |
| CN105075845B (en) * | 2015-08-24 | 2018-01-05 | 温州大学 | The selection of high-quality sargassum fusifome |
| CN105532421A (en) * | 2015-12-16 | 2016-05-04 | 胡彩军 | Method for artificially producing sargassum fusiforme seedlings in freshwater |
| CN107155865A (en) * | 2017-07-10 | 2017-09-15 | 温州市洞头区水产科学技术研究所 | A kind of method for propagating sargassum fusifome artificially |
| CN110122312A (en) * | 2019-03-11 | 2019-08-16 | 华南理工大学 | A method of improving sargassum fusifome growth of seedling |
| CN110122312B (en) * | 2019-03-11 | 2021-05-14 | 华南理工大学 | Method for improving growth of seedlings of sargassum fusiforme |
| CN110852647A (en) * | 2019-11-09 | 2020-02-28 | 温州大学 | Quality grade classification method for air bag outlet products of cultivated sargassum fusiforme |
| CN110852647B (en) * | 2019-11-09 | 2024-04-09 | 温州大学 | Quality grade classification method for airbag outlet products of cultured sargassum fusiforme |
| CN112806251A (en) * | 2021-02-26 | 2021-05-18 | 温州市洞头区海洋与渔业发展研究中心 | Unit mass evaluation and difference comparison method for cultivating juvenile sporophytes of sargassum fusiforme |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104620969B (en) | 2017-01-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104620969B (en) | Rapid breeding method of Sargassum fusiforme | |
| Hurtado et al. | Developments in production technology of Kappaphycus in the Philippines: more than four decades of farming | |
| US7484329B2 (en) | Technology for cultivation of Porphyra and other seaweeds in land-based sea water ponds | |
| US7080478B2 (en) | Technology for cultivation of Porphyra and other seaweeds in land-based sea water ponds | |
| CN103070076B (en) | One method of cultivating peanut Vitro Mutation directed screening salt tolerant body | |
| CN103749302A (en) | Induced acclimation and cultivation method for salt-tolerant bamboo reed seedlings | |
| CN102939898B (en) | Method for catalpa bungei clonal seed selection | |
| CN104255441B (en) | A kind of selection of spring hybrid stem lump mustard | |
| CN104094832B (en) | Method for crossbreeding of undaria pinnatifida by using unisexual planospores | |
| CN104719162A (en) | Method for screening dandelion salt-tolerance mutants | |
| CN104137769B (en) | A kind of wild cabbage utilizes the method for the DH system disease-resistant new quality variety of seed selection | |
| CN102150612B (en) | Method for breeding new variety of leaf achillea | |
| CN101717748B (en) | Method for generating macrogametocyte by somatic cell of inducing undaria pinnatifida parthenogenesis for juvenile sporophyte | |
| CN105010126A (en) | Fast breeding method for black spot resistant sweet potato variety | |
| CN101703003B (en) | Method for quickly propagating grassiness | |
| CN107155879B (en) | A kind of black pine aseptic and rapid propagation method | |
| US20080083160A1 (en) | Compositions of enriched seaweeds in land-based sea water ponds | |
| CN103999680B (en) | Plasma treatment willow branch produces the method for bud mutation | |
| CN102771386B (en) | Breeding method of rice | |
| CN103828710A (en) | Efficient chrysanthemum cross-breeding method | |
| CN107950399B (en) | Method for improving utilization and screening efficiency of potato seedling seeds | |
| Islam et al. | Effects of growth regulators on in vitro propagation and tuberization of four Dioscorea species | |
| CN106577282B (en) | A method of using terminal bud culture epipremnum aureum | |
| CN104686370B (en) | Method for cultivating maca polyploid plants | |
| Luhan et al. | Growth and carrageenan quality of sporophyte and gametophyte of the commercially important red seaweed Kappaphycus alvarezii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |