CN109601373A - A kind of spring wild cabbage resists the selection of underdone bolting early-maturing variety - Google Patents

A kind of spring wild cabbage resists the selection of underdone bolting early-maturing variety Download PDF

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Publication number
CN109601373A
CN109601373A CN201910043848.5A CN201910043848A CN109601373A CN 109601373 A CN109601373 A CN 109601373A CN 201910043848 A CN201910043848 A CN 201910043848A CN 109601373 A CN109601373 A CN 109601373A
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wild cabbage
early
spring
resists
maturing variety
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张恩慧
许忠民
杨安平
孙庆国
张鑫鑫
蔡美杰
李岸
王谦
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Northwest A&F University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses the selections that a kind of spring wild cabbage resists underdone bolting early-maturing variety, are related to breeding technical field.The method are as follows: it is female parent that the spring wild cabbage, which resists underdone bolting early-maturing variety to be with DH10-ZG48CMS, using DH13-20M3 as male parent, is obtained by hybridization, and the spring early 1852 is named as.It with holding is to form after DH10-ZG48M hybridizes through 5 generation backcross transformations that the DH10-ZG48CMS, which is using wild cabbage Ogura cytoplasm male sterility line,.It is described holding be DH10-ZG48M be by wild cabbage isolated microspore culture technique, using wild cabbage precocity cenospecies " it is desirable that " microspores culture initiative DH system.The DH13-20M3 is that " Isolated microspore of sweet 50 " of the Qin is formulated using wild cabbage early-maturing variety.Spring for being selected using the method for the present invention early 1852 is a kind of to resist underdone bolting, high-quality, disease-resistant, high yield spring wild cabbage precocity new varieties.

Description

A kind of spring wild cabbage resists the selection of underdone bolting early-maturing variety
Technical field
The present invention relates to the breeding sides that breeding technical field more particularly to a kind of spring wild cabbage resist underdone bolting early-maturing variety Method.
Background technique
Wild cabbage is one of the main vegetables crop of the more season cultivations in China various regions and year-round supply, wild cabbage battalion rich in Substance and cancer-resisting substance are supported, to promoting human health and uplift the people's living standard to play an important role.Wild cabbage polymorphic type Breed breeding can realize year-round supply, play a crucial role to alleviating market dull and rush season, meeting the stable market supply of fresh vegetable anniversary.90 years After generation, China's wild cabbage cultivated area growth rate is very fast, is especially increased with spring early maturing cabbage cultivated area and is protruded the most, when Preceding about 91.6 ten thousand hectares of cultivated area of the wild cabbage in the whole nation, wherein early spring wild cabbage cultivated area almost cultivate by shared half, early spring wild cabbage It has the advantage that one is the field growing phase is short, about needs 55 days or so, plant high financial profit;The second is wild cabbage resists cold, feature is early Spring cultivating and growing favorably alleviates the problem of spring market lacks dish;The third is early spring wild cabbage cultivates advantageous crop rotation and improves field of vegetables Multiple crop index.
Early spring wild cabbage cultivation industry faces the excellent variety that main problem is a lack of anti-bolting, due in China's spring wild cabbage The period is cultivated, the head cabbage varieties that Yi Fasheng " cold spell in later spring " causes spring habit weak occur " underdone bolting " phenomenon by vernalization, make it Spring wild cabbage cultivation meaning is lost, thus the anti-bolting breed breeding of spring wild cabbage is classified as wild cabbage breeding objective by country, based on the domestic spring Wild cabbage industry demand and national spring wild cabbage breeding objective require breeding to resist underdone bolting, high-quality, disease-resistant, 1.1 kilograms of left sides Dan Qiuchong The right side, field planting become the breeding objective of the breed breeding to 55~58 days early maturing cabbage new varieties of harvest.
Summary of the invention
In view of this, the embodiment of the invention provides the selection that a kind of spring wild cabbage resists underdone bolting early-maturing variety, it is main Syllabus is to select high-quality, disease-resistant, precocious wild cabbage new varieties.
In order to achieve the above objectives, invention broadly provides following technical solutions:
On the one hand, described the embodiment of the invention provides the selection that a kind of spring wild cabbage resists underdone bolting early-maturing variety Spring wild cabbage early-maturing variety is with DH10-ZG48CMS for female parent, using DH13-20M3 as male parent, is obtained by hybridization, is named as the spring Early 1852.
Preferably, DH10-the ZG48CMS is using wild cabbage Ogura cytoplasmic male sterile line and to keep system After DH10-ZG48M hybridization, formed through 5 generation backcross transformations.
Preferably, it is to utilize wild cabbage morning by wild cabbage isolated microspore culture technique that the holding, which is DH10-ZG48M, Ripe cenospecies " it is desirable that " microspores culture initiative DH system.
Preferably, the DH13-20M3 is that " Isolated microspore of sweet 50 " of the Qin is formulated using wild cabbage early-maturing variety.
Preferably, the DH13-20M3 first cultivates DH13-20 plants, next year obtains DH13-20M3 system, through field agriculture Skill Characters Identification is determining and obtains.
Compared with prior art, the beneficial effects of the present invention are:
The present invention is female parent with DH10-ZG48CMS cytoplasmic male sterile line, with DH13-20M3 dihaploid self-mating system A kind of new varieties " spring early 1852 " are obtained by hybridization for male parent, parents are dihaploid self-mating system (DH system);It is maternal It is using wild cabbage Ogura cytoplasmic male sterile line with holding is to be formed after DH10-ZG48M hybridizes through 5 generation backcross transformations;Its Male parent DH13-20M3 is that " Isolated microspore of sweet 50 " of the Qin is formulated using wild cabbage early-maturing variety;The field of its first generation of hybrid It is high to show uniformity, plant forms half are upright, and outer leaf green, blade face is smooth, and wax powder is few, and leaf-head is emerald green, resist underdone bolting (early Sowing 5 days or meet cold spell in later spring plant bolting rate is 0) resistance to blastomere, and just, commodity is good for leaf-head circle, and leaf-head appearance meets consumer's habit Used, the crisp sweet tea of ball blade quality, flavor quality is excellent, viral diseases and black rot, kind stable high yield;" spring early 1852 " is a kind of Resist underdone bolting, high-quality, disease-resistant, high yield spring wild cabbage precocity new varieties.
Detailed description of the invention
Fig. 1 is the maternal DH10-ZG48CMS Breeding Process figure that the embodiment of the present invention 1 provides;
Fig. 2 is the maternal DH10-ZG48CMS field growing figure that the embodiment of the present invention 1 provides;
Fig. 3 is the male parent DH13-20M3 Breeding Process figure that the embodiment of the present invention 1 provides;
Fig. 4 is the male parent DH13-20M3 field growing figure that the embodiment of the present invention 1 provides;
Fig. 5 is head cabbage varieties " spring early 1852 " Breeding Process figure that the embodiment of the present invention 1 provides;
Fig. 6 is head cabbage varieties " spring early 1852 " field growing figure that the embodiment of the present invention 1 provides.
Specific embodiment
For further illustrate the present invention to reach the technical means and efficacy that predetermined goal of the invention is taken, below with compared with Good embodiment, to specific embodiment, technical solution, feature and its effect applied according to the present invention, detailed description is as follows.Under Stating the special characteristic, structure or feature in multiple embodiments in bright can be combined by any suitable form.
Technical term of the present invention is explained as follows:
Cytoplasmic male sterility (cytoplasmic male sterility, CMS): it is to be widely present in higher plant One of natural phenomena, it is normal to show as maternal inheritance, pollen abortion and Stamen development, growth.
Isolated microspore culture technique: refer to without any type of anther preculture, directly obtained from bud or flower medicine The method for taking the fresh microspore group of free state to be cultivated.
Dihaploid (DH, i.e. Doubled Haploid): it is added by the haploid gamete manual cell induction of diploid The dliploid individuality generated after times or the dliploid individuality generated by female/male development of gametid orphan of tetraploid.
The following female parent DH10-ZG48CMS and male parent DH13-20M3 used in Example 1 of the present invention are selected from northwest agriculture Initiative product is independently studied in College of Horticulture, woods University of Science and Technology wild cabbage breeding research room, and the public can obtain sample from the research department.
Embodiment 1
1. parent's breeding:
(1) maternal breeding: maternal DH10-ZG48CMS cytoplasmic male sterile line is to utilize wild cabbage Ogura cytoplasmic male not Educating be with holding is after DH10-ZG48M hybridizes, and Jing Duodai backcross transformation forms;As shown in Figure 1, 2;Wherein, holding is DH10- ZG48M be by wild cabbage isolated microspore culture technique, using wild cabbage precocity cenospecies " it is desirable that " microspore-isolated culture create The DH system of system;Maternal DH10-ZG48CMS cytoplasm male sterility line phene is stablized, and sterility is thorough, blooms normal, nectary Prosperity, stamen are all degenerated, and gynoecium is normal, and plant strain growth is healthy and strong, low temperature not yellow, no pollen grain, plant and floral organ under room temperature It being all gone well in addition to sterility, kind strain branchiness is stronger, average kind of plant height 88.3cm, and the florescence 38~40 days;Leaf-head is round, center Column 5.6cm, the leaf-head degree of packing 0.65, siphonal lobe is yellowish green, and wax powder is medium, and maturity is precocious, and spring outdoor cropping field planting was by harvest 58 days;It is anti- Underdone bolting, viral diseases and black rot resistance, tipburn, resistance to blastomere, coordinate force are high.
(2) male parent breeding: male parent DH13-20M3 be using wild cabbage early-maturing variety " Isolated microspores of sweet 50 " of the Qin formulate and At;As shown in Figure 3,4, DH13-20 plants of acquisition in 2010, acquisition DH13-20M3 system in 2011;Through field agriculture in 2011~2012 years Skill Characters Identification, highly ordered in the character that 2012 are bred as, gene inheritance is stable, is one resists underdone bolting, disease resistance Strong dihaploid self-mating system;Maturity is precocious, and spring outdoor cropping field planting was by harvest 52 days, leaf color yellow green, and wax powder is few;Leaf-head circle Shape, newel 4.5cm, the leaf-head degree of packing 0.64;Viral diseases and black rot resistance, tipburn, resistance to blastomere;Reproductive stage plant Branchiness is strong, and average kind of plant height 100.5cm, the florescence 35 days or so, floral organ was normal, and pollen amount is big, and coordinate force is high.
2. combination selection: 2012, according to hybrid parents matching principle in wild cabbage breeding, start selection with DH10- 8 cytoplasm male sterility lines such as ZG48CMS are female parent, select, coordinate force excellent with the economic characters including DH13-20M3 high and Winter habit is strong, resistance to blastomere, and the strong totally 9 DH systems of disease resistance or self-mating system are male parent, hybridizes combo and researchs and analyses to coordinate force (such as Fig. 5) was tried through 2013~2014 years on too white etc. the ground in College of Horticulture, Xibei Univ. of Agricultural & Forest Science & Technology experimental plot and Xi'an, Baoji It tests, as a result, it has been found that DH10-ZG48CMS × DH13-20M3 combination performance hybrid vigour is significant (such as Fig. 6), integral economic trait is excellent It is good, resist underdone bolting, quality, disease resistance and yield to be superior to check variety " 8398 " (being shown in Table 1).
1. wild cabbage of table " spring early 1852 " main target character comparison result
" spring early 1852 " wild cabbage early-maturing variety is the spring wild cabbage breeding of new varieties target established according to country and criterion for breeding, By the character to spring wild cabbage quality breeding material, homozygous, identification, cytoplasmic male sterility transformation and coordinate force compare screening, will Bolting resistance quality disease resistance and yielding ability polymerize improved variety together, and variety characteristic meets national breeding objective and production Industry needs, and is an improved Varieties, main innovation point is as follows:
1. resist underdone bolting, it is best in quality: through 2013-2015 product than investigation and analysis and Agricultural Products in Shaanxi quality surveillance Inspection point's test analysis, spring early 1852 kinds with compare " 8398 " variety protection, resist underdone bolting strong, fallen with chance within early sowing 5 days Cold spell in spring, bolting rate are 0, and best in quality, ball leaf is emerald green, the crisp sweet tea of the food value of leaf, newel length 4.8cm, shorter than control 1.1cm;Leaf-head The degree of packing 0.72 is greater than control 0.14;It helps leaf than 21.8%, is less than control 2.5%;Nutrition Quality Analysis, dry matter, solubility Sugar, crude fibre and crude protein are above control, and respectively 1.15%, 0.47%, 0.29% and 0.25% (are shown in Table 2).
The anti-bolting of 2. spring of table early 1852 kind objective traits and quality qualification result
2. high and stable yields, yielding ability is strong: spring early 1852 kinds with compare 8398 variety protections, through variety test, area Domain test and production demonstration, continuous 5 years yielding abilities are prominent, spring early 1852 average product 3789.3kg/667m2, than control 8398 Kind volume increase 12.0%.
(1) variety test: by 2013~2015 years continuous 3 years variety tests as a result, 3 year morning in spring of discovery 1852 kind per mu yields are 3691.1kg/667m2, 8398 per mu yield of check variety is 3296.8kg/667m2, morning in spring 1852 than control 12.0% (being shown in Table 1) of volume increase.
(2) regional testing: by continuous 2 years 2015~2016 years inside the province regional testing as a result, 2 year spring of discovery early 1852 Kind per mu yield 3789.3kg/667m2, 8398 per mu yield of check variety is 3381.7kg/667m2, spring early 1852 ratio Control volume increase 12.0%;Early 1852 strain's area experiments of spring in 2015, in Hanzhong, point is more maximum than control amount of increase in production, volume increase 16.2%, , the volume increase 8.9% more minimum than control amount of increase in production in too white point;Early 1852 strain's area experiments of spring in 2016, compare in Yang Lingdian ratio and increase Production amplitude is maximum, volume increase 15.3%, and in Hu County, point is more minimum than control amount of increase in production, increases production 7.9% (being shown in Table 3).
3. spring of table early 1852 regional experiment of variety yield results
(3) production demonstration: by 2015~2016 years continuous biennial production demonstration yield results, the discovery spring early 1852 was average Per mu yield 3643.2kg/667m2, 8398 per mu yield of check variety is 3295.6kg/667m2, the spring early 1852 is than control volume increase 10.6% (being shown in Table 4).
4. spring of table early 1852 kinds pilot production demonstration result over the years
3. resistance to blastomere, high disease resistance: being identified through many years of field, it is found that evening receives 5 days blastomere indexes less than 3 grades, blastomere rate is small In 5%, resistance to blastomere;It is identified through field resistance, high antiviral disease, black rot and tipburn (being shown in Table 5).
5. spring of table early 1852 kind objective trait disease resistances and resistance qualification result
4. tree characteristics neat and consistent: spring early 1852 kinds are to replace self-mating system as parents using wild cabbage DH system earliest to match The precocious spring wild cabbage of the first generation of hybrid produced resists underdone bolting kind, and the high consistency of DH system genotype makes " spring early 1852 " product Plant forms, leaf color and the balling sex expression for planting field are highly ordered.
The present invention is that spring wild cabbage is precocious by spring early 1852 (DH10-ZG48CMS × DH13-20M3) that the above method is bred as First generation of hybrid kind, field planting was by harvest 56 days or so;Utilize wild cabbage cytoplasm male sterility line DH10-ZG48CMS and DH system DH13- 20M3 hybridization, which is prepared, to be bred as, and parents are dihaploid self-mating system (DH system), and first generation of hybrid variable rate technology uniformity is high, plant shape State half is upright, and outer leaf green, blade face is smooth, and wax powder is few, and leaf-head is emerald green;38.2~39.1cm of plant development degree, the outer number of sheets 10 ~11, Dan Qiuchong 1.1kg or so, leaf-head is round, the leaf-head degree of packing 0.72, newel length 4.8cm, high by 1/2 less than ball, side Leaf is than 21.8%.Resist underdone bolting, early sowing 5 days or meets cold spell in later spring bolting, high antiviral disease, black rot and dry combustion method does not occur The heart, resistance to blastomere, just, commodity is good for leaf-head circle, and leaf-head appearance meets consumer's habit;The crisp sweet tea of ball blade quality, flavor quality are excellent It is good.Kind stable high yield, average product 3789.3kg/667m2, than 8398 kinds of control volume increase 12.0%, the spring early 1852 is one A anti-bolting High quality and diseases resistance high-yield variety of spring wild cabbage.
Place, those skilled in the art can not select from the prior art to the greatest extent in the embodiment of the present invention.
Disclosed above is only a specific embodiment of the invention, but scope of protection of the present invention is not limited thereto, is appointed What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with above-mentioned scope of protection of the claims It is quasi-.

Claims (5)

1. the selection that a kind of spring wild cabbage resists underdone bolting early-maturing variety, which is characterized in that the spring wild cabbage early-maturing variety is It is female parent with DH10-ZG48CMS, using DH13-20M3 as male parent, is obtained by hybridization, is named as the spring early 1852.
2. the selection that a kind of spring wild cabbage as described in claim 1 resists underdone bolting early-maturing variety, which is characterized in that described It is to return after DH10-ZG48M hybridizes through 5 generations that DH10-ZG48CMS, which is using wild cabbage Ogura cytoplasmic male sterile line and holding, Transformation is handed over to form.
3. the selection that a kind of spring wild cabbage as claimed in claim 2 resists underdone bolting early-maturing variety, which is characterized in that described Holding be DH10-ZG48M be by wild cabbage isolated microspore culture technique, using wild cabbage precocity cenospecies " it is desirable that " microspore Cultivate the DH system of initiative.
4. the selection that a kind of spring wild cabbage as claimed in claim 3 resists underdone bolting early-maturing variety, which is characterized in that described DH13-20M3 is that " Isolated microspore of sweet 50 " of the Qin is formulated using wild cabbage early-maturing variety.
5. the selection that a kind of spring wild cabbage as claimed in claim 4 resists underdone bolting early-maturing variety, which is characterized in that described DH13-20M3 first cultivates DH13-20 plants, and next year obtains DH13-20M3 system, identifies through Agronomic characteristic and determines and obtain.
CN201910043848.5A 2019-01-17 2019-01-17 A kind of spring wild cabbage resists the selection of underdone bolting early-maturing variety Pending CN109601373A (en)

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Application publication date: 20190412