CN101904295A - Convergent cross breeding method for multi-resistance high-quality orange heart baby cabbage for spring and autumn - Google Patents
Convergent cross breeding method for multi-resistance high-quality orange heart baby cabbage for spring and autumn Download PDFInfo
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Abstract
The invention discloses a convergent cross breeding method for multi-resistance high-quality orange heart baby cabbage for spring and autumn. In order to solve problems, the method comprises the following steps of: selectively breeding a high-stalk encircling resistance source 322; selectively breeding an encircling orange heart material 02-693; selectively breeding a premature orange heart self-bred line 08P238; hybridizing a TuMV resistance source B75 and Fushan wrapped cabbage head, and then performing multi-generation inbred to obtain a property modified encircling resistance source 95; selectively breeding a bolting-resistant material 538; selectively breeding an extremely early orange heart strain 304; selectively breeding an orange heart baby cabbage self-bred line X08-219; performing coordinating test on the premature orange heart self-bred line 08P238 and the orange heart baby cabbage self-bred line X08-219; transforming the premature orange heart self-bred line 08P238 into a sterile line to obtain an orange heart male sterile line 08P238A; and hybridizing the orange heart baby cabbage self-bred line X08-219 and the orange heart male sterile line 08P238A to obtain an F1 crossbreed. The orange heart baby cabbage selectively bred by the method for spring and autumn is extremely early, has high virus disease resistance and orange color, and is suitable to be cultivated in spring and autumn.
Description
Technical field
The present invention relates to a kind of cross breeding method of doll's dish, the convergent cross breeding method of the tangerine heart baby dish of particularly a kind of multi-resistance high-quality dual-purpose in spring and autumn.
Background technology
The heterosis utilization approach is substantially all adopted in present domestic Chinese cabbage breeding; Since the eighties in 20th century, domestic each breeding units has successively been bred large quantities of autumn Chinese cabbage half-blood, and has realized that ripe property of morning, noon and afternoon is supporting, and storage tolerance, product are of fine quality, disease resistance is strong etc. has their own characteristics each, but the kind that integrates these advantages seldom.Improve the quality of Chinese cabbage, enrich the designs and varieties of Chinese cabbage, satisfy the demand of each side, become the fresh target of breeding.In general, Chinese cabbage by cultivation season can be divided into spring Chinese cabbage, summer Chinese cabbage, early autumn cabbage (dealer Chinese cabbage) and the autumn and winter Chinese cabbage; By ripe property can be divided into extremely early mature, precocity, in ripe and late-maturing; Be divided into subsphaeroidal, head shoot shape (taper), shell shape and straight tubular by sphere; Be divided into again by the obvolvent type and folded embrace, fill the span of a man's arms, to twist armful and comfortable etc.The Chinese cabbage cultivar of anti-season spring, summer cultivation is mainly derived from Korea S and Japan and other countries at present, and its shortcoming is that the ability of viral diseases and anti-tipburn is relatively poor, and vegetative period is longer; The spring breeds of Chinese cabbage bred of China has a small amount of listing in recent years, but further improves remaining aspect bolting resistant property and the output.The summer breeds of Chinese cabbage of north high temperature resistance arid, its research work is shouldered heavy responsibilities.Development along with China's storing and the foreign trade industry, the increase in Chinese cabbage special product district, corresponding storage tolerance breed breeding becomes important breeding objective, storage tolerance kind on producing at present, the part that comes with some shortcomings decreases in recent years as the viral diseases ability, in the saline land cultivation or manage and easily produce tipburn when bad, not strong to soft rot and black rot resistance, need further improve and improve.In the spring breeds of Chinese cabbage of northern China spring and high and cold mountain area extensive plantation in summer, though bolting resistant property is stronger, disease resistance, quality and storage property are demanded urgently improving and improving at present.Cultivation high-quality, the comprehensive good Chinese cabbage cultivar of proterties are still the direction of long-term endeavour from now on; Cultivate new, strange, special, high nutrition or have the kind of special color, local flavor, as gold zone, tangerine heart kind, become Chinese cabbage breeding for quality fresh target, each breeding units is all being actively developed the research of this breeding technique.For the needs of supermarket and outlet, research cultivates that high-quality, balling consolidation, outward appearance are beautiful, storage tolerance, the long middle-size and small-size kind (as doll's dish) of shelf life become new target.From eating requirements, cultivate eat raw succulence tender and crisp, rinse that pot is easily ripe, the big soft specific breeds such as kind and fast food of boarding of leaf also just more and more come into one's own.The annual Chinese cabbage quantity of producing of China is very big, and deep processing is one of Chinese cabbage outlet, and along with the development of Chinese cabbage processing industry, the demand of processing the tailored version kind accordingly will be one of new target of breeding.Processing variety generally require the output height, nutritious, dry matter content is high, but different processing methods also is not quite similar to the demand of Chinese cabbage cultivar, so development processing tailored version kind is very important targetedly.The seed selection of high disease and insect resistance kind is one of most important target of Chinese cabbage breeding forever, use disease-resistant variety not only saving of work and time but also the good characteristic that can express its kind, can effectively reduce spray medicine number of times in addition, be easy to produce green or nuisanceless safe and high quality product, this is to selling and exporting all most important.Applying along with new improved variety in addition, pathogen also can morph and produce new biological strain (strain system), make resistant lose, less important disease also can rise to main disease or produce new disease etc. simultaneously, and breeding for disease resistance is the target that the Chinese cabbage breeding is adhered to all the time.Economic characters such as Chinese cabbage output are desalinated gradually, oppose that mutually its quality and edible safety and health requirement are more and more higher.
From the breeding technique aspect, several production of hybrid seeds approach of Chinese cabbage crossing use of advantage mainly contain " selfing is not affine ", " high for inbred line " and " male sterile " etc. at present.Wherein " selfing is not affine " still is present main production of hybrid seeds approach; " male sterile two-purpose line " is because its limitation is superseded substantially; " cytoplasmic sterility " and " nuclear gene is done sterile mutually " owing to be convenient to control, the high-quality crossbreed that is easy to get, characteristics such as with low cost development rapidly, especially " cytoplasmic sterility " just becoming the forward position of domestic and international research.The seed selection of OguCMS cytoplasm male sterility line is succeedd, by how generation backcrosses to novel radish sterile cytoplasm source and from the cabbage type rape cytoplasmic male sterilty material hybridization of France's introduction with Chinese cabbage, obtain no yellow, it is normal to blossom and bear fruit, sterile plant rate and sterile degree reach 100% cytoplasmic male sterile line, and have good coordinate force.PolCMS cytoplasm male sterility line research aspect utilizes the RAPD technology that the gene DNA of male sterile line of cabbage CMS3411-7 and its maintenance line 3411-7 is compared research, has found male sterile line and maintenance line specific amplified band separately.Genetic male sterile line research aspect is guidance with " multiple allele heredity hypothesis ", and the genic male sterile gene that just will come from the straight barrel type Chinese cabbage is to type of filling the span of a man's arms and the transformation of forward type material, successfully with this sterile gene transformation in Chinese cabbage and a variety of Chinese cabbage.Radiation breeding and polyploid breeding are also further developed in addition, the collection of germ plasm resource more and more comes into one's own with innovation, Chinese cabbage microspores culture technology is improved, and has all obtained major progress at aspects such as the improvement of material preliminary treatment, medium, operating technology simplification, microspore regeneration rates; Aspect the cabbage molecule marker-assisted breeding, by microspores culture, the segregation population that has obtained 100 double haploid systems is used for the structure of Chinese cabbage linkage map; The linkage group that acquisition equates with the Chinese cabbage chromosome number.With Chinese cabbage nuclear Dominant Male Sterility Gene material is the examination material, adopt BSA method and RAPD technology, obtained and the closely linked RAPD mark of male sterile gene, successfully the RAPD mark is converted into the SCAR mark, this mark will help accelerating the transformation speed of Chinese cabbage nuclear Dominant Male Sterility Gene.The research of disease resistance authenticate technology has obtained further developing, and the pathogen of new disease is cultivated and the artificial infection technology obtains new development and perfect.The genetics of resistance rule of various diseases has obtained further research, disease-resistant gene molecule marker be positioned with tremendous development, provide assurance for identifying the breeding for disease resistance material accurately and quickly.The raising that the raising of stock breeding rules and quick test authentication method and seed processing, storage technique and improvement will promote seed quality.Supporting technology research of breeding good method and the perfect quick popularization that will promote new varieties.China has obtained remarkable achievement in the breeding for disease resistance field, especially the aspects such as anti-source material evaluation, collection and genetic breeding of aspects such as viral diseases, downy mildew, soft rot.
Domesticly carry out tangerine heart breeding for disease resistance and start late, successfully breed the tangerine heart Chinese cabbage cultivar that is fit to autumn culture, but the domestic tangerine heart baby dish new varieties report that is fit to the plantation in spring and autumn that yet there are no, the tangerine heart baby dish that indivedual units breed only is fit to positive season cultivation, and the difficult problem of unresolved viral diseases all the time, breeding time is long, and individual plant is too big, and ball-type is embraced for folded, not too be fit to do the cultivation of doll's dish, its economic characters have big gap with gold zone doll's dish that Japan, Korea S etc. breed.Tangerine heart baby dish new varieties breed the anniversary stable market supply that can facilitate tangerine heart baby dish.
States such as Japan carry out the Chinese cabbage crossing breeding early, and especially the anti-season Chinese cabbage is than China decades early spring, summer, the spring of breeding, summer Chinese cabbage dominated China anti-season Chinese cabbage seeds market.Japan, Korea S etc. have bred gold zone, tangerine heart Chinese cabbage seriation spring Chinese cabbage and autumn and winter breeds of Chinese cabbage in recent years.Because Japanese disease is light, lack the anti-source of TuMV, it is the difficult problem of unresolved viral diseases all the time, and artificial infection China Chinese cabbage main producing region TuMV main flow strain system and field planting are identified and are all reached high sense level, can't realize stable high yield in China at all.The tangerine heart baby vegetable of anti-season such as Japan, Korea S kind is not appeared in the newspapers yet.It is more that Japan and Korea S breed the kind of gold zone doll dish, but breeding time is long, and individual plant is excessive, can only urge it and diminish by strengthening planting density, causes the siphonal lobe waste too much, and the clean vegetables rate is low, the reduction of field biopotency.The also difficult problem of unresolved viral diseases of the type kind also can't realize high and stable yield in grave illness districts such as Jinan in addition.Because the healthy nutritive value of gold zone, especially tangerine core white dish uniqueness and the appetitive effect of beautiful color and luster energy, Japan, Korea S etc. are all competitively developing colored Chinese cabbage seriation new varieties, eliminate common Chinese cabbage cultivar gradually.
Summary of the invention
The problem to be solved in the present invention provides the convergent cross breeding method of the tangerine heart baby dish of a kind of multi-resistance high-quality dual-purpose in spring and autumn, make it pass through parent's combo order, evaluation and screening method and material application study in the pyramiding breeding technology, required multiple excellent genes is gathered in one, thereby select many anti-, high-quality, the tangerine heart baby dish of dual-purpose type in spring and autumn.
For addressing the above problem, the present invention includes following steps:
(1) with special 826 hybridization of the anti-source BP007 of TuMV and the anti-source of TuMV stone after inbreeding of more generation carries out anti-TuMV screening, and screening clean vegetables rate height, plant type is compact and group is thin strain system, the anti-source 322 of high stake encircle-class of character improvement;
(2) the anti-source 322 of height stake encircle-class is hybridized with the tangerine psychological treatment meals spring after inbreeding of more generation carries out anti-TuMV screening, get the tangerine heartwood material of encircle-class 02-693;
(3) encircle-class dried tangerine peel heartwood material 02-693 is hybridized with Ych-H-1-357 after inbreeding of more generation carries out anti-TuMV screening again, and the strong and precocious strain system of screening bolting resistant property, precocious tangerine heart inbred line 08P238 got;
(4) with the hybridization of the anti-source B75 of TuMV and packet header, Fushan after inbreeding of more generation carries out anti-TuMV screening, and screening clean vegetables rate height, plant type is compact and group is thin strain system, the anti-source 95 of encircle-class of character improvement;
(5) with the anti-source of encircle-class 95 with the mini hybridization of the tangerine heart after inbreeding of more generation carries out anti-TuMV screening, and the strong strain system of screening bolting resistant property, the material of anti-bolting the 538;
(6) big good fortune 65 and the anti-source of summer Chinese cabbage group 1 are hybridized the back inbreeding of more generation and carry out anti-TuMV screening, and screening is precocious and the individual plant of the tangerine heart, getting the tangerine heart strain of extremely early mature is 304;
(7) be that 304 hybridization back inbreeding of more generation carry out anti-TuMV screening with the tangerine heart strain of material of anti-bolting the 538 and extremely early mature, and the strong and precocious strain of screening bolting resistant property is to get tangerine heart baby dish inbred line X08-219;
(8) precocity tangerine heart inbred line 08P238 and tangerine heart baby dish inbred line X08-219 are carried out combining ability test, screening obtains tangerine heart baby dish combination;
(9) the precocious tangerine heart inbred line 08P238 that selects in the step 8 and nucleo-cytoplasmic interaction sterile source CMA are carried out many for the backcross transformation male sterile line, tangerine heart male sterile line 08P238A;
(10) with tangerine heart baby dish inbred line X08-219 and the tangerine heart male sterile line 08P238A hybridization selected in the step 8, the seed of adopting on the tangerine heart male sterile line 08P238A gets the tangerine heart baby dish of multi-resistance high-quality dual-purpose in spring and autumn F1 crossbreed.
In order to reduce breeding cycle and to guarantee the accuracy of anti-source seed selection, the inbreeding of more generation in described step (1)-(7) be 4-6 for selfing, returning on behalf of 4-6 generation of step (9) backcrossed more.
In order to improve the screening accuracy rate, anti-TuMV screening technique is in described step (1)-(7): after carrying out artificial inoculation on seedling TuMV Shandong 2 strains system from selfing 1 and the 2 generations beginning, select the plant field planting field of 0 and 1 grade of the sick level of TuMV, screen virus disease, downy mildew and the soft rot three serious diseases level of falling ill then in the field again and be 0 and 1 strain system.
Beneficial effect of the present invention: (1) utilizes repeatedly convergent cross breeding technique, in conjunction with artificial infection and season ecologic planting evaluation the carrying out screening of disease resistance and economical character more than a year, domestic anti-source material and external improved seeds or material are repeatedly added hybridization, backcross, good plants such as high antiviral disease, bolting resistant property, tangerine disposition shape, extremely early mature and clean vegetables rate are learned proterties and economic characters polymerization in one, the tangerine heart inbred line of the special small-sized wide coordinate force of acquisition; Aspect the screening of inbred line disease resistance, utilize many seasons different ecological condition emphasis that different diseases is screened, again in conjunction with multiple choice methods such as artificial infection and biological characters, having worked out a cover is to select main line with the disease resistance, with parents' objective trait difference minimum is principle, with objective trait heritability powerhouse is the premature polymerization emphasis, and the rapid breeding technology of hybridization and selfing selection has repeatedly overcome the defective of present single selection.In first national TuMV anti-source transformation being arrived on the tangerine heart baby dish both at home and abroad, select and be fit to dual-purpose tangerine heart baby dish in spring and autumn, its extremely early mature, 40 ~ 45 days breeding times, the high antiviral disease is identified in artificial infection (TuMV Shandong 2 strains system) and field, multiple diseases such as double downy mildew resistance, soft rot, tipburn, three big disease field disease indexs are lower than 1.0 ~ 5.0.High 18 ~ the 20cm of ball, the thick 5 ~ 7cm of ball, the dense planting individual plant weighs 200 ~ 250g, about thin planting 1Kg; Ball leaf Chinese red, not easy to change during storing, anti-bolting is fit to spring and autumn (13-32 ℃) cultivation, at grave illness district and second crop soil performance high and stable yield.Tangerine heart baby dish carotin and vitamin C equal size are higher than 1.2 ~ 4.0 times of common Chinese cabbage, the soluble sugar content height, flavor quality is good, be fit to stir-fry and eat with cold and dressed with sauce, cook soup etc., anti-accumulating, easily advantage such as packing.Short 15 ~ 50 days of breeding time of the more common Chinese cabbage of tangerine heart baby dish, and have stronger disease-resistant, resistance, can continuous many batches of intensive cultivatings, improve land utilization rate.Doll's dish is compared with common Chinese cabbage, and the field biopotency improves greatly, has reduced unnecessary loss, can reduce the soil nutrient loss, and culture fertility is realized the sustainable development of China's agricultural.Tangerine heart baby dish gold zone doll dish is at present compared high anti-multiple diseases, breeding time is short, under the situation of not using any agricultural chemicals, can realize high and stable yield, for pollution-free food and organic farming production provide basic assurance, also carry out green and organic market vegetables and export goods and earn foreign currency strong technical support is provided for tangerine heart baby dish.
(2) utilize the production of hybrid seeds of nucleo-cytoplasmic interaction type CMA male sterile line, the seed purity of producing can reach 100%, fundamentally solved because of use self incompatible line or affine be that the production of hybrid seeds causes the not high technical barrier of seed purity; In the domestic success of transformation the earliest doll dish CMA nucleo-cytoplasmic interaction type male sterile line, this male sterile line sterile plant rate and sterile degree all reach 100%, and nectary is normal, seed collecting amount height, and seed purity can reach 100%.
(3) at present gold zone doll dish disease resistance is strong, littler compacter, the more current gold zone doll's famished look of plant type pool is more gorgeous, nutritive value is higher to educate tangerine heart baby dish, has overcome the shortcoming that latter's color is subject to such environmental effects simultaneously.The present every 666.7m of gold zone doll dish
2Can multiple 2000-5000, increase benefit 500-1000 unit.
Description of drawings
Fig. 1 is the breeding collection of illustrative plates of the specific embodiment of the invention one.
Fig. 2 is the breeding collection of illustrative plates of the specific embodiment of the invention two.
Embodiment
Specific embodiment one
The convergent cross breeding method of the tangerine heart baby dish of a kind of multi-resistance high-quality dual-purpose in spring and autumn as shown in Figure 1, it comprises the steps:
(1) with anti-source BP007 of TuMV and special 826 hybridization back selfing of the continuous 5 generations screening of the anti-source of TuMV stone; After carrying out artificial inoculation on seedling TuMV Shandong 2 strains system from selfing 1 and the 2 generations beginning, select the plant field planting field of 0 and 1 grade of the sick level of TuMV, carry out comprehensive resistance (select virus disease, downy mildew, soft rot three serious diseases fall ill level be 0 and 1 plant), economic characters (selecting excellent strains such as clean vegetables rate height, plant type compactness, group are thin) then in the field again and wait comprehensively screening, high the anti-source 322 of encircle-class TuMV of character improvement.
The anti-source BP007 of TuMV is high stake straight barrel type material, 90 days breeding times, plant height 51.8cm, the long 52.4cm of leaf, the wide 19.4cm of leaf, petiole white, thick 0.7cm, 7 strains are that artificial infection reaches immunity and high anti-level to 19 main flow separators of domestic TuMV, the highest disease index has only 0.56, but not downy mildew resistance and soft rot etc., the economic characters extreme difference is a little less than the bolting resistant property.
Folded embrace the anti-source of class TuMV stone special 85 days 826 breeding times, leaf does not have burr, king-pile is folded embrace slightly be back taper, high antiviral disease, hold concurrently anti-soft rot, downy mildew etc., economic characters are good, in the bolting resistant property.
The anti-source of high stake encircle-class 322 proterties are high the comfortable shape of filling the span of a man's arms, artificial infection TuMV Shandong 2 strains system reaches high anti-level, and downy mildew resistance, soft rot etc. are about 85 days breeding times, leaf does not have burr, white group, middle rib Boping, economic characters significant improvement has been arranged, but bolting resistant property still a little less than.
(2) with the anti-source 322 of height stake encircle-class with tangerine psychological treatment meals spring hybridization after continuous 6 generations selfing screen the tangerine heartwood material of encircle-class 02-693; At first in 2 strains of artificial inoculation on seedling TuMV Shandong be in its selfing 1 and 2 generations, the plant field planting field that the sick level of screening TuMV is 0 and 1 grade, carry out comprehensive resistance (select virus disease, downy mildew, soft rot three serious diseases fall ill level 0 and 1 plant), economic characters (selecting excellent strains such as clean vegetables rate height, plant type compactness, group are thin) then again waits and comprehensively screens in the field, again respectively at the screening of carrying out summer such as soft rot etc., the tangerine heartwood material of the encircle-class 02-693 of character improvement.
The tangerine psychological treatment meals spring for fill the span of a man's arms or detain embrace a garden column type, through artificial infection and field resistance identification research, the high susceptible viral disease of result, even late sowing to August 20, also usually has no harvest, in addition its breeding time longer, be 75 days; But the clean vegetables rate is higher, the tangerine heart, and bolting resistant property is stronger, and downy mildew resistance and soft rot etc. are better.
The tangerine heartwood material of encircle-class 02-693 is the king-pile shell shape of filling the span of a man's arms, through artificial infection and field resistance identification research, high antiviral disease, hold concurrently downy mildew resistance, soft rot etc., economic characters are basic identical with the tangerine psychological treatment meals spring, 75-80 days breeding time, have root and carefully help slender lobule to wait the characteristics of the typical number of sheets type Chinese cabbage of filling the span of a man's arms more, but anti-bolting is medium.
(3) with the tangerine heartwood material of encircle-class 02-693 again with Ych-H-1-357 hybridization after continuous 5 generations selfing screen precocious tangerine heart inbred line 08P238; At first in 2 strains of artificial inoculation on seedling TuMV Shandong be in its selfing 1 and 2 generations, the plant field planting field that the sick level of screening TuMV is 0 and 1 grade, carry out comprehensive resistance (select virus disease, downy mildew, soft rot three serious diseases fall ill level 0 and 1 plant), economic characters excellent strains such as () choosing precocity, clean vegetables rate height, plant type compactness, group are thin etc. then more comprehensively screens in the field, select the individual plant of the strong and anti-tipburn of bolting resistant property in spring, select the individual plant of anti-soft rot in summer, the precocious tangerine heart inbred line 08P238 of character improvement.
Ych-H-1-357 is shorter breeding time, and about 65 days, bolting resistant property is stronger, and it is fast to fill heart speed, clean vegetables rate height, downy mildew resistance, soft rot, tipburn etc., but viral diseases not.Length breeding time of the tangerine heartwood material of encircle-class 02-693, the shortcoming a little less than the bolting resistant property have been overcome by the precocious tangerine heart inbred line 08P238 of hybridizing back pedigree seed selection.
(4) the hybridization continuous 6 generations selfing in back of the anti-source B75 of TuMV and packet header, Fushan is screened the anti-source 95 of encircle-class of character improvement; At first in 2 strains of artificial inoculation on seedling TuMV Shandong be in its selfing 1 and 2 generations, the plant field planting field that the sick level of screening TuMV is 0 and 1 grade, carry out comprehensive resistance (select virus disease, downy mildew, soft rot three serious diseases fall ill level 0 and 1 plant), economic characters (selecting excellent strains such as clean vegetables rate height, plant type compactness, group are thin) then in the field again and wait comprehensively screening, the anti-source 95 of encircle-class that gets character improvement.The anti-source B75 of TuMV be Beijing 75 kinds through 5 generation the height that forms of selfed breeding for inbred line, the number of sheets is few, helps thickly, economic characters are poor, not anti-soft rot and downy mildew, tipburn etc.
Packet header, Fushan is the Shandong landrace, but about 75 days breeding times, viral diseases, more anti-tipburn, soft rot, downy mildew, economic characters are ungood, bolting resistant property is strong, meet 15-32 ℃ of ability bolting below 13 ℃ after 20-30 days blooms.
The anti-source of encircle-class is about 95 75 days breeding times, the white heart, the king-pile shell shape of filling the span of a man's arms, and high antiviral disease, the downy mildew resistance etc. of holding concurrently, economic characters have significant improvement than the anti-source B75 of TuMV, and bolting resistant property also increases significantly.
(5) with the anti-source of encircle-class 95 with the mini hybridization of the tangerine heart after continuous 5 generations selfing screen the material of anti-bolting the 538; At first in 2 strains of artificial inoculation on seedling TuMV Shandong be in its selfing 1 and 2 generations, the plant field planting field that the sick level of screening TuMV is 0 and 1 grade, carry out comprehensive resistance (select virus disease, downy mildew, soft rot three serious diseases fall ill level 0 and 1 plant), economic characters (selecting excellent strains such as clean vegetables rate height, plant type compactness, group are thin) then again waits and comprehensively screens in the field, select the individual plant of the strong and anti-tipburn of bolting resistant property in spring, select the individual plant of anti-soft rot in summer, the material of anti-bolting the 538 of character improvement.
The tangerine heart mini (ORANGE MINI), about 65 days breeding times, bolting resistant property is strong, clean vegetables rate height, anti-tipburn etc., but viral diseases not.The gained material of anti-bolting the 538 has had significant shortening than the anti-source 95 of encircle-class on breeding time, plant type is compacter, and bolting resistant property also increases.
(6) with big good fortune 65 with 1 hybridization of the anti-source of summer Chinese cabbage group after continuous 6 generations selfing and carry out disease resistance, prematurity is screened to such an extent that the tangerine heart strain of extremely early mature is 304; At first in 2 strains of artificial inoculation on seedling TuMV Shandong be in its selfing 1 and 2 generations, the plant field planting field that the sick level of screening TuMV is 0 and 1 grade, carry out comprehensive resistance (select virus disease, downy mildew, soft rot three serious diseases fall ill level 0 and 1 plant), economic characters (selecting clean vegetables rate height, plant type compactness, individual little, excellent strain such as group is thin) then again waits and comprehensively screens in the field, respectively at selecting anti-soft rot, tipburn in autumn and summer, reaching precocious individual plant, get the tangerine heart 304 of extremely early mature of character improvement again.
Big good fortune 65 bolting resistant properties are stronger, downy mildew resistance, tipburn etc., but viral diseases not.
The heat-resisting extremely early mature that the anti-source of summer Chinese cabbage group 1 obtains through the selfing screening of 5 generations for little assorted 50 kinds, folded armful of head shoot shape, breeding time is extremely short, and 40-45 days, the inbred line that plant type is less.
The tangerine heart 304 of the extremely early mature that obtains of screening is the shell shape of filling the span of a man's arms, extremely early mature, but bolting resistant property a little less than, high antiviral disease, soft rot etc.
(7) with the tangerine heart strain of material of anti-bolting the 538 and extremely early mature 304 hybridization after continuous 6 generations selfing and carry out resistance screening and get tangerine heart baby dish inbred line X08-219; At first in 2 strains of artificial inoculation on seedling TuMV Shandong be in its selfing 1 and 2 generations, the plant field planting field that the sick level of screening TuMV is 0 and 1 grade, carry out comprehensive resistance (select virus disease, downy mildew, soft rot three serious diseases fall ill level 0 and 1 plant), economic characters (selecting clean vegetables rate height, precocity, individual little, excellent strain such as plant type is compact, group is thin) etc. then more comprehensively screens in the field, select the individual plant of the strong and anti-tipburn of bolting resistant property in spring, select the individual plant of anti-soft rot in summer, the tangerine heart baby dish inbred line X08-219 of character improvement.
Tangerine heart baby dish inbred line X08-219 has had raising on bolting resistant property, and stronger warm tolerance is arranged, sick downy mildew resistance, the soft rot etc. held concurrently of high antiviral, and plant type is minimum, about 40 days breeding times, has the distinctive proterties of doll's dish.
(8) precocity tangerine heart inbred line 08P238 and tangerine heart baby dish inbred line X08-219 are carried out combining ability test, and respectively at spring, plantation in autumn, with Korea S gold zone doll dish shellfish shellfish jade is contrast, by survey producing and the disease index comparative analysis significantly is better than contrasting and screens the combination that obtains tangerine heart baby.
(9) precocious tangerine heart inbred line 08P238 and the nucleo-cytoplasmic interaction sterile source CMA that selects in the step 8 carried out 4-5 for the backcross transformation male sterile line, get tangerine heart male sterile line 08P238A.
(10) with tangerine heart baby dish inbred line X08-219 and the tangerine heart male sterile line 08P238A hybridization selected in the step 8, the seed of adopting on the tangerine heart male sterile line 08P238A gets the tangerine heart baby dish of multi-resistance high-quality dual-purpose in spring and autumn F1 crossbreed.
Specific embodiment two
The convergent cross breeding method of the tangerine heart baby dish of a kind of multi-resistance high-quality dual-purpose in spring and autumn as shown in Figure 2, it comprises the steps:
(1) with after anti-source BP007 of TuMV and special 826 hybridization of the anti-source of TuMV stone, carries out anti-TuMV screening, and screen thin strain system of clean vegetables rate height, plant type compactness and group, get the anti-source 322 of height stake encircle-class of character improvement through the selfing of 3 generations;
(2) the anti-source 322 of height stake encircle-class is hybridized with the tangerine psychological treatment meals spring after anti-TuMV screening is carried out in the selfing of 5 generations, get the tangerine heartwood material of encircle-class 02-693;
(3) encircle-class dried tangerine peel heartwood material 02-693 is hybridized with Ych-H-1-357 after anti-TuMV screening is carried out in the selfing of 4 generations again, and the strong and precocious strain system of screening bolting resistant property, precocious tangerine heart inbred line 08P238 got;
(4) with the hybridization of the anti-source B75 of TuMV and packet header, Fushan after anti-TuMV screening is carried out in the selfing of 7 generations, and screening clean vegetables rate height, plant type is compact and group is thin strain system, the anti-source 95 of encircle-class of character improvement;
(5) with the anti-source of encircle-class 95 with the mini hybridization of the tangerine heart after anti-TuMV screening is carried out in the selfing of 5 generations, and the strong strain system of screening bolting resistant property, the material of anti-bolting the 538;
(6) big good fortune 65 and the anti-source of summer Chinese cabbage group 1 are hybridized back 7 generations selfing and carry out anti-TuMV screening, and screening is precocious and the individual plant of the tangerine heart, getting the tangerine heart strain of extremely early mature is 304;
(7) be that anti-TuMV screening is carried out in 304 hybridization back 4 generations selfings with the tangerine heart strain of material of anti-bolting the 538 and extremely early mature, and the strong and precocious strain of screening bolting resistant property is to get tangerine heart baby dish inbred line X08-219;
(8) precocity tangerine heart inbred line 08P238 and tangerine heart baby dish inbred line X08-219 are carried out combining ability test, screening obtains the combination of tangerine heart baby dish;
(9) the precocious tangerine heart inbred line 08P238 that selects in the step 8 and nucleo-cytoplasmic interaction sterile source CMA were carried out for 3 generations and backcross, the transformation male sterile line, tangerine heart male sterile line 08P238A;
(10) with tangerine heart baby dish inbred line X08-219 and the tangerine heart male sterile line 08P238A hybridization selected in the step 8, the seed of adopting on the tangerine heart male sterile line 08P238A gets the tangerine heart baby dish of multi-resistance high-quality dual-purpose in spring and autumn F1 crossbreed.
The high antiviral disease is identified in tangerine heart baby dish artificial infection that above-mentioned two specific embodiments are bred (TuMV Shandong 2 strains system) and field, the multiple diseases such as downy mildew resistance, soft rot, tipburn of holding concurrently; Three big disease field disease indexs are lower than 1.0 ~ 5.0.Extremely early mature, 40 ~ 45 days breeding times, the dense planting individual plant weighs 200 ~ 250g, about thin planting 1Kg; The tangerine heart, anti-bolting is fit to spring and autumn (13-32 ℃) cultivation, at grave illness district and second crop soil performance high and stable yield; Carotin and vitamin C equal size are higher than 1.2 ~ 4.0 times of common Chinese cabbage, the soluble sugar content height, and flavor quality is good, is fit to stir-fry and eat, cooks soup and cold and dressed with sauce etc., anti-accumulating, easily advantage such as packing.
Claims (3)
1. the convergent cross breeding method of the tangerine heart baby dish of multi-resistance high-quality dual-purpose in spring and autumn is characterized in that: comprise the steps:
(1) with after anti-source BP007 of TuMV and special 826 hybridization of the anti-source of TuMV stone, carries out anti-TuMV screening, and screen thin strain system of clean vegetables rate height, plant type compactness and group, get the anti-source 322 of height stake encircle-class of character improvement through inbreeding of more generation;
(2) the anti-source 322 of height stake encircle-class is hybridized with the tangerine psychological treatment meals spring after inbreeding of more generation carries out anti-TuMV screening, get the tangerine heartwood material of encircle-class 02-693;
(3) encircle-class dried tangerine peel heartwood material 02-693 is hybridized with Ych-H-1-357 after inbreeding of more generation carries out anti-TuMV screening again, and the strong and precocious strain system of screening bolting resistant property, precocious tangerine heart inbred line 08P238 got;
(4) with the hybridization of the anti-source B75 of TuMV and packet header, Fushan after inbreeding of more generation carries out anti-TuMV screening, and screening clean vegetables rate height, plant type is compact and group is thin strain system, the anti-source 95 of encircle-class of character improvement;
(5) with the anti-source of encircle-class 95 with the mini hybridization of the tangerine heart after inbreeding of more generation carries out anti-TuMV screening, and the strong strain system of screening bolting resistant property, the material of anti-bolting the 538;
(6) big good fortune 65 and the anti-source of summer Chinese cabbage group 1 are hybridized the back inbreeding of more generation and carry out anti-TuMV screening, and screening is precocious and the individual plant of the tangerine heart, getting the tangerine heart strain of extremely early mature is 304;
(7) be that 304 hybridization back inbreeding of more generation carry out anti-TuMV screening with the tangerine heart strain of material of anti-bolting the 538 and extremely early mature, and the strong and precocious strain of screening bolting resistant property is to get tangerine heart baby dish inbred line X08-219;
(8) precocity tangerine heart inbred line 08P238 and tangerine heart baby dish inbred line X08-219 are carried out combining ability test, screening obtains the combination of tangerine heart baby dish;
(9) precocious tangerine heart inbred line 08P238 and the nucleo-cytoplasmic interaction sterile source CMA that selects in the step 8 carried out how generation backcrosses, the transformation male sterile line gets tangerine heart male sterile line 08P238A;
(10) with tangerine heart baby dish inbred line X08-219 and the tangerine heart male sterile line 08P238A hybridization selected in the step 8, the seed of adopting on the tangerine heart male sterile line 08P238A gets the tangerine heart baby dish of multi-resistance high-quality dual-purpose in spring and autumn F1 crossbreed.
2. breeding method according to claim 1 is characterized in that: the inbreeding of more generation in described step (1)-(7) be 4-6 for selfing, returning on behalf of 4-6 generation of step (9) backcrossed more.
3. breeding method according to claim 1, it is characterized in that: it is characterized in that: anti-TuMV screening technique is in described step (1)-(7): after carrying out artificial inoculation on seedling TuMV Shandong 2 strains system from selfing 1 and the 2 generations beginning, select the plant field planting field of 0 and 1 grade of the sick level of TuMV, screen virus disease, downy mildew and the soft rot three serious diseases level of falling ill then in the field again and be 0 and 1 strain system.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172210A (en) * | 2011-02-24 | 2011-09-07 | 山东春秋大白菜育种研究中心 | Breeding method for high-resistance TuMV late bolting yellow core spring cabbages |
| CN102172211A (en) * | 2011-02-24 | 2011-09-07 | 山东春秋大白菜育种研究中心 | Multi-resistant high-quality Qingmaye type red-orange-core Chinese cabbage breeding method |
| CN109601373A (en) * | 2019-01-17 | 2019-04-12 | 西北农林科技大学 | A kind of spring wild cabbage resists the selection of underdone bolting early-maturing variety |
| CN109924124A (en) * | 2019-04-01 | 2019-06-25 | 江苏丘陵地区镇江农业科学研究所 | A method of lasting powdery-mildew-resistance wheat breeding element is formulated based on multiple gene polymerization |
-
2010
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172210A (en) * | 2011-02-24 | 2011-09-07 | 山东春秋大白菜育种研究中心 | Breeding method for high-resistance TuMV late bolting yellow core spring cabbages |
| CN102172211A (en) * | 2011-02-24 | 2011-09-07 | 山东春秋大白菜育种研究中心 | Multi-resistant high-quality Qingmaye type red-orange-core Chinese cabbage breeding method |
| CN109601373A (en) * | 2019-01-17 | 2019-04-12 | 西北农林科技大学 | A kind of spring wild cabbage resists the selection of underdone bolting early-maturing variety |
| CN109924124A (en) * | 2019-04-01 | 2019-06-25 | 江苏丘陵地区镇江农业科学研究所 | A method of lasting powdery-mildew-resistance wheat breeding element is formulated based on multiple gene polymerization |
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