CN106305413A - Breeding method of maize inbred line S37 - Google Patents

Abstract

The invention relates to a breeding method of maize inbred line S37, and belongs to the technical field of agricultural science. The breeding method comprises the following steps: (1) using Shen 137 as a female parent and MO17 as a male parent for hybridization to obtain an F1 generation of Shen 137/MO17; (2) inbreeding the F1 generation to obtain an F2 generation of Shen 137/MO17; (3) using an early-maturing density-resistant material FR3 as a female parent and the F2 generation of Shen 137/MO17 as a male parent for hybridization to obtain the F1 generation of FR3/Shen 137/MO17; (4) selecting excellent individual plants from the F1 generation of FR3/Shen 137/MO17 as the female parent, using the early-maturing density-resistant material FR3 as the female parent for backcrossing to obtain the F1 generation of FR3/FR3 Shen 137/MO17; (5) continuously inbreeding the F1 generation of FR3/FR3 Shen 137/MO17 for six generations to breed the maize inbred line S37. The S37 bred according to the method provided by the invention has the advantages of high combining ability, moderate growth period, good plant type, good density resistance, high disease resistance and wide adaptability, has become one of excellent inbred lines commonly used in our country, and is an important basic material for the improvement of maize inbred lines and the hybridization breeding in the future.

Description

A kind of selection of corn inbred line S37

Technical field

The invention belongs to technical field of agriculture science, be specifically related to the selection of a kind of corn inbred line S37.

Background technology

The eighties in 20th century, the pandemic such as the leaf blight of corn, graywall and curving cascade, spread Red jade 13, Yedan No.13 number etc. are compact, Semi-compact maize kind is susceptible serious and exits production.The nineties, China from External introducing contains torrid zone blood relationship kind matter, and then it is high to have selected a collection of single productive forces, disease-resistanting leaf spot, the kind that blade is denseer Matter.Semen Maydis tikka class disease is played effective inhibitory action by it, but a disadvantage is that the ripe phase is the most late.And along with disease bion Change, the prolongation of application time, only cannot persistently go down by monogenic resistance.After entering 2000, domestic and international breeder Think that the further of corn yield is improved, dense planting need to be leaned on to increase spike number, improve seed-producing rate, utilize population effect to increase production, promote me State's corn breeding, production and cropping system reach a new level again.But finding in Sheng Chaning, strengthen density, that brings is not only disease Seriously, lodging more and more heavier, the impact on yield is huge.In recent years, China main maize area corn germ plasm resource heredity The trend that basis is narrow is more and more obvious, the most domestic precocity, resistance to close, multiresistance corn inbred line shortage.

Summary of the invention

The present invention solves the problems of the prior art, it is provided that one combines with single-gene and polygenic resistance, anti-list One disease and anti-multiple diseases combine, the selection of multiresistance corn inbred line S37.

In order to solve above-mentioned technical problem, technical scheme is specific as follows:

The selection of a kind of corn inbred line S37, comprises the following steps:

(1) with Shen 137 for maternal, MO17 is male parent, carries out hybridization and obtains the F1 generation of Shen 137/MO17；

(2) F1 generation selfing obtains the F2 generation of Shen 137/MO17；

(3) with precocious resistance to close material FR3 as female parent, the F2 of Shen 137/MO17, on behalf of male parent, carries out hybridization and obtains FR3/ Shen The F1 generation of 137/MO17；

(4) selecting fine individual plant in the F1 generation of FR3/ Shen 137/MO17 is male parent, with precocious resistance to close material FR3 as mother This, carrying out backcrossing obtains the F1 generation of FR3/FR3 Shen 137/MO17；

(5) corn inbred line S37 is selected after F1 generation continuous selfing 6 generation of described FR3/FR3 Shen 137/MO17.

In technique scheme, the resistance to close material FR3 of described precocity is the precocious resistance to close cenospecies in Europe.

The invention has the beneficial effects as follows:

1, the selection of the corn inbred line S37 that the present invention provides is material based on Shen 137 and MO17, and MO17 has Having combining ability high, comprehensive agronomy character is good, and the advantage such as heritability is strong, but its disease resistance is poor；And Shen 137 disease resistance is good, wide Suitable, the advantage of the two selfing line is aggregating, the Elite inbred that selection-breeding height is anti-, outside acceding state, precocious resistance to close material, makes Material becomes dense planting precocious, resistance to.

2, the selection Nan Fanbei of corn inbred line S37 that the present invention provides educate, suitably encryption, artificial vaccination disease-resistant The method identified, increases natural selection and artificial selection's pressure, improves resistance to close ability and disease resistance.The selection of small group in employing Method, makes the relatively large colony of the procedure of breeding select more simply, easily operate, and chance of success is bigger.

3, the selection that the present invention provides selects S37 combining ability height, moderate growth duration, plant type are delicate and pretty, High planting Good, disease resistance by force, is extensively fitted, it has also become one of Elite inbred that China is conventional.S37 is improvement of maize inbred lines and hybridization from now on The important foundation material of breeding.

Accompanying drawing explanation

With detailed description of the invention, the present invention is described in further detail below in conjunction with the accompanying drawings.

Fig. 1 is the breeding line spectrogram of the corn inbred line S37 of the present invention.

Detailed description of the invention

The invention thought of the present invention is: the eighties in last century, China's corn leaf spot is popular, and breeding circle starts to educate disease-resistant Plant and paid attention to.In succession bring out some single-genes disease-resistant selfing line the nineties, but be as the change of disease bion, during application Between prolongation, only cannot persistently go down by monogenic resistance.In recent years, China main maize area corn germ plasm resource hereditary basis Narrow trend is more and more obvious, the most domestic precocity, resistance to close, multi-resistant corn selfing line shortage.To this end, the present invention proposes with list The breeding for disease resistance of the multiresistance that gene and polygenic resistance combine, anti-single disease and anti-multiple diseases combine, with precocious, Resistance to close, multi-resistance is the general thought of Breeding direction.

The present invention proposes Core Germplasms carries out improvement innovation, by backcrossing and the technological means such as composite-crossing, imports heat Band blood relationship kind matter, introduces foreign country's germ plasm resource simultaneously, innovates domestic germ plasm resource.Determine with containing torrid zone blood relationship, the P group of resistance Elite inbred MO17 in the Lancaster group of one of the big miscellaneous excellent group of (Shen 137) and China four is object of study, outside acceding state Precocious resistance to close material increases prematureness and High planting, improved agronomic traits.Select close, multi-resistance precocious, resistance to, combining ability high, Adaptable material.MO17, as the representative of Lancaster kind matter, has that combining ability is high, seed-producing rate is high, best in quality, fertility Phase is moderate, the Core Germplasms that barren tolerance and the advantage, always China's large area eighties such as drought tolerance is good use, but it feels curved spore Bacterium leaf spot.Shen 137 selfing line contains torrid zone blood relationship, disease resistance and lodging resistance and highlights, and shortcoming is that the ripe phase is later.Utilization backcrosses and turns Method of educating imports the Shen 137 containing Tropical germplsam, synthesizes basic population, improves Core Germplasms hereditary basis.By colony, high density, Coercing by force the technology such as selection-breeding of shuttling back and forth with strange land, comprehensive disease resistance, yielding ability, lodging resistance, High planting are by generation strict screening and analyze, After setting up disease-resistant basic material, import external precocious material FR3 close, good grain quality precocious, resistance to and be added to improve in colony, logical Cross the mode of composite-crossing, be added to improve in colony by prematureness, High planting.

Through 3 years 6 generations, carry out selecting between many head progeny rows, strain by pedigree method, utilize artificial disease-resistant inoculated identification, south Numerous north is educated, and improves disease resistance and adaptability.Retain disease-resistant, hold that green property is good, anti-fall, plant is delicate and pretty, stem and leaf angle is little, blade narrow and Straight and upright, the less plant of tassel branch；And root system, fruit ear and resistance etc. are oriented selection.Finally bring out combining ability high, raw Phase of educating is moderate, plant type is delicate and pretty, High planting is good, disease resistance is strong, the Elite inbred S37 of wide adaptability.And assembled Jilin Province and The corn hybrid seed Ji east 22 of Heilungkiang two province authorization and the lucky east 807 of entrance Jilin Province authorization test.

Describe embodiments of the invention the most in detail:

Illustrate, in conjunction with Fig. 1, the selection of corn inbred line S37 that the present invention provides: winter Hainan in 1997, use Shen 137 and MO17 constructional base materials, and with Shen 137 for maternal, MO17 is male parent, carries out hybridizing the F1 obtaining Shen 137/MO17 Generation.Liaoyuan in 1998, F1 generation selfing, obtain the F2 generation of Shen 137/MO17.The precocious resistance to close material in winter in 1998 Hainan plantation Europe In the F2 generation of FR3 and Shen 137/MO17, carry out hybridization and obtain the F1 generation of FR3/ Shen 137/MO17.1999 in Liaoyuan with FR3/ Shen Selecting fine individual plant in the F1 generation of 137/MO17 is male parent, and with Europe, precocious resistance to close material FR3 is as female parent, carries out the acquirement that backcrosses The F1 generation of FR3/FR3 Shen 137/MO17, selects selfing line S37 after continuous selfing 6 generation (S1-S6), particularly as follows: winter sea in 1999 South plantation selfing line 1000 strain, bagging roguing selfing.Liaoyuan pedigree method in 2000, selects excellent head progeny row selfing, accelerates there is niche Because separating and restructuring.Period of duration is through the character identification such as disease-resistant, anti-fall, and leafing angle is little, blade is narrow and upper punch, tassel branch are few in screening Strain, by its fruit ear list fringe threshing during results.30 head progeny rows that Hainan in 2000 will be chosen in the north, subregion sows, flower Phase selects blade narrow, short and plant few, the subramose bagging selfing of tassel pollen amount.Elite plant strain is carried out simultaneously leaf blight, Curving cascade and head smut are manually identified.Disease-resistant, anti-fall, plant type compact delicate and pretty fine individual plant is retained during results 30.

Survey combines, and accurately selects high-combining ability strain, carries out miscellaneous excellent group and sorts out, accelerates breeding hybridized process.

Calendar year 2001, Hainan was through continuous selfing and selection, and character is the most basicly stable, while selecting selfing line, changed (prosperous with Huang 7-2), the selfing line such as Lucia Red Cob (R34) and Reid (7922) is that Testers is measured.Within 2002, the test cross kind to Hainan is divided Not in Liaoyuan, the ground such as Tieling and Dandong carry out qualification test, carry out preliminary identification, result shows that the combining ability in 2000 wide 5th district is high, Its Test Crosses kind shows high yield, disease-resistant, plant type is more delicate and prettier.The most prominent with S37 × R34 yield, the most selected head progeny row continues Selfing is isozygotied, and strengthens Testers simultaneously and is measured.The multiple character such as plant forms now has been stablized and neat and consistent.

The comparison of the S37 that the method that the present invention provides selects and Present Domestic Lancaster blood relationship system MO17:

MO17 is the Inbred Lines in Spring maize seeding district of China, and combining ability is high, and Comprehensive Traits is excellent, has been bred as a large amount of excellent Cenospecies.S37 is material based on Shen 137 and MO17, through how forming for many head progeny rows self cross and selection, combining ability, economical character, Disease resistance, lodging resistance and ripe phase aspect have had bigger improvement, have assembled out disease resistance and the excellent cenospecies of lodging resistance.

S37 with MO17 and Shen 137 are compared:

S37 combines the excellent genes of the two, and strain plant type is delicate and pretty, and plant height is shorter than MO17,199 centimetres, 70 lis, fringe position Rice, has more lodging resistance, tassel branch about 3-5, and branch's number is few, reduces the consumption to nutrient.In disease resistance, heredity Shen The disease-resistant gene of 137, anti-various leaf spots, bar of living is ripe, under artificial inoculation conditions, anti-corn leaf spot, head smut, relatively Anti-Pyrausta nubilalis (Hubern)..From period of duration, more Zao than Shen 137, period of duration 123 days, need >=10 DEG C of accumulated temperature about 2500 DEG C, ripe selfing in genus System.In terms of rice matter and yield, seed cutin type, 100-grain weight 26.9 grams, marketing quality is excellent, parent propagation field general hectare yield It is more than 3800 kilograms, belongs to excellent high yield selfing line.

S37 applicable cases:

Lucky east 22:

Lucky east 22 is with S37 as female parent, and R34 is the middle cooked maize cenospecies that male parent assembles, and within 2004, participates in Ji Woods saves Semen Maydis and gives standby test, and average product is 11415.2 kilograms/hectare, averagely increases production 18.8% than check variety.2005- Within 2006, participating in the examination of Jilin Province maize district, average product is 9807.7 kilograms/hectare, averagely increases production 14.8% than check variety. 2006 participate in Jilin Province's pilot production average product is 10624.9 kilograms/hectare, increases production 14.9% than check variety.2006 Year variety certification committee of Jilin Province authorization.Within 2010, participating in Heilongjiang Province's introduction and Experiment, average hectare yield is 8753.7 public affairs Jin, averagely increases production 10.8% than check variety.Within 2011, participate in Heilongjiang Province two district's pilot production, average hectare yield 8706.8Kg, averagely increases production 8.6% than check variety.

Lucky east 807:

Lucky east 807 is with S37 as female parent, and F69 is the middle cooked maize cenospecies that male parent assembles, and within 2011, participates in the Liao Dynasty Academy of Agricultural Sciences of source city produces ratio test, and average product is 12397.2 kilograms/hectare, averagely increases production 12.8% than check variety, Within 2012, participating in Liaoyuan City Academy of Agricultural Sciences and produce ratio test, average product is 11964.4 kilograms/hectare, average than check variety Volume increase 9.3%.

At present, the selfing line with S37 as parent, in forthcoming years, hybridization can be applied to by more corn breeder Plant in selection-breeding and inbred line breeding, will effectively play its high yield, disease-resistant, anti-fall feature.

Obviously, above-described embodiment is only for clearly demonstrating example, and not restriction to embodiment.Right For those of ordinary skill in the field, can also make on the basis of the above description other multi-form change or Variation.Here without also cannot all of embodiment be given exhaustive.And the obvious change thus extended out or Change among still in the protection domain of the invention.

Claims (2)

1. the selection of a corn inbred line S37, it is characterised in that comprise the following steps:

(1) with Shen 137 for maternal, MO17 is male parent, carries out hybridization and obtains the F1 generation of Shen 137/MO17；

(2) F1 generation selfing obtains the F2 generation of Shen 137/MO17；

(3) with precocious resistance to close material FR3 as female parent, the F2 of Shen 137/MO17, on behalf of male parent, carries out hybridization and obtains FR3/ Shen 137/ The F1 generation of MO17；

(4) selecting fine individual plant in the F1 generation of FR3/ Shen 137/MO17 is male parent, with precocious resistance to close material FR3 as female parent, enters Row backcrosses and obtains the F1 generation of FR3/FR3 Shen 137/MO17；

(5) corn inbred line S37 is selected after F1 generation continuous selfing 6 generation of described FR3/FR3 Shen 137/MO17.

The selection of corn inbred line S37 the most according to claim 1, it is characterised in that the resistance to close material of described precocity FR3 is the precocious resistance to close cenospecies in Europe.