CN108925423A - A kind of heat-resisting germplasm selection of Rise's boot period - Google Patents
A kind of heat-resisting germplasm selection of Rise's boot period Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention relates to rice varieties to cultivate field, in particular to a kind of heat-resisting germplasm selection of Rise's boot period, comprising the following steps: long-grained nonglutinous rice conventional variety (restorer) is used as receptor, and heat-resisting rice germplasm system obtains F1 generation as donor, hybridization；F1 generation and receptor are returned 4-6 times, obtain BCnF1 generation；In BCnF1 generation, is selfed, and while selfing, high temperature stress is carried out, until the boot stage for obtaining stabilization characteristics of genetics heat-resisting germplasm.This method hybridized for 1 generation with donor by receptor, it was returned for 5 generations, it was selfed for 3 generations again, separation offspring is generated by selfing, in conjunction with field natural high-temperature stress and phjytotron simulation high temperature stress identification, the height that boot stage is heat-resisting, yielding ability is good is selected to carry out species test for Backcross introgression family in advanced backcross analysis, it selects through boot stage after high temperature stress is handled, setting percentage is higher than 10% or more recurrent parent, the new germ plasm that comprehensive agronomy trait expression and the consistent boot stage heat resistance of recurrent parent significantly improve.
Description
Technical field
The present invention relates to rice varieties to cultivate field, in particular to a kind of heat-resisting germplasm breeding side of Rise's boot period
Method.
Background technique
In recent years, in the overall situation of global warming, China's rice Climatic regionalization frequently occurs.In rice reproductive growth
Phase, continuous more light sammings are greater than 35 DEG C or more weather and constantly occur, cause rice the significantly underproduction (Yang Huicheng etc.,
2004).Some researches show that boot stage and full heading time are the rice period most sensitive to high temperature stress (Matsui T et
al.2001).Rice can cause the degeneration of grain husk flower or pollen sterility by high temperature stress in boot stage and lead to Severe Reduction (Meng Ya
Benefit etc., 1997).Effective pollen is when Deng Yun etc. (2010) discovery heading with the continuous 3d of pollen mother cell meiosis and 1d thereafter
(microspore early stage) pollen activity of high-temperature process is minimum, i.e. the high temperature sensitive phase in boot stage is that meiophase and microspore are early
Phase.This period meets with high temperature and often results in that development of floral organs is not complete, and pollen development is bad, vigor decline, Rice Flowering loose powder and
Pollen tube growth is obstructed, and causes to be fertilized and form empty grain.
In view of this, the present invention is specifically proposed.
Summary of the invention
The purpose of the present invention is being directed to the above-mentioned deficiency of the prior art, a kind of excavate and using from Oryza glaberrima Steud is provided
Resource IRGC102309 boot stage heat-resisting gene, it is significantly heat-resisting that breeding is suitable for season semilate rice plantation regional boot stage on the south the Changjiang river
Middle indica rice variety breeding.
For the harm of boot stage high temperature stress in Xian conventional variety in reply and hybrid rice production, it is resistance to select boot stage
High temperature, high yield the Xian new hybrid rice varieties in the middle Xian conventional variety of one and two systems, can be effectively reduced high temperature stress pair
The harm of boot stage hybrid rice, to increase safeguard for grain security.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of heat-resisting germplasm selection of Rise's boot period, comprising the following steps:
In indica type conventional kind (restorer) be used as receptor, heat-resisting rice germplasm system hybridized as donor, obtains F1
Generation；
F1 generation and receptor are returned 4-6 times, obtain BCnF1 generation；
In BCnF1 generation, is selfed, and while selfing, high temperature stress is carried out, until the boot stage for obtaining stabilization characteristics of genetics is resistance to
Hot germplasm.
It is more that boot stage high temperature resistant, the breeding difficulty of high-quality, high yield multiple target character are had both in Rice Production at present, it is main
If because lacking the genetic resources for having both these characters in existing backbone parent；The followed by existing preferable kind of heat resistance
Hereditary basis is narrow；And the presence between the Main Agronomic Characters such as heat resistance and yield, quality is negatively correlated.It is heat-resisting to obtain boot stage
Property the breakthrough varieties significantly improved, the gene in effective use boot stage significant heat-resisting germ plasm resource becomes reliable choosing
It selects.
Short tongue wild rice of the Oryza glaberrima Steud originating from Africa, is one of two rice cropping kinds.It is provided as rice germplasm
The important materials that source utilizes, compared with Asian Cultivated Rice, Oryza glaberrima Steud low output is only limitted to West Africa Sub-Sahara
Area plantation.Jiangxi Province super rice research and development center finds that wherein numbering is IRGC102309 in heat resistance Screening germplasm
The Oryza glaberrima Steud boot stage heat-resisting germplasm line of (Oryza glaberrima Steud.) is donor (International Rice institute of Philippine
There is provided), middle Xian conventional variety Yang No.6 rice, as two-line hybrid rice parent also known as 9311 (O.sativa subsp.indica)
For recurrent parent, hybridized by 1 time, 5 backcrossings, 3 selfings, cultivates the high generation backcrossing that a collection of IRGC102309 is donor and lead
Enter and is.The favorable genes of digging utilization boot stage of the present invention extremely heat-resisting germplasm line IRGC102309, are combined artificial intelligence
The means of high temperature stress treatment appraisal are simulated to the young fringe of pollen mother cell meiosis and microspore early stage in greenhouse, according at
The performance of ripe phase setting percentage selects boot stage heat resistance than new germ plasm system (setting percentage that recurrent parent Yang No.6 rice significantly improves
10% or more) higher than recurrent parent.Further field natural high-temperature stress is carried out in Agriculture in Jiangxi academy of sciences Nanchang proving ground
After identification, it was demonstrated that new germplasm line boot stage heat-resisting sex expression is significantly better than recurrent parent really, may be directly applied to produce
Practice.
Rice restorer boot stage heat-resisting germplasm selection provided by the invention, hybridized for 1 generation with donor by receptor, returned
It handed over for 5 generations, then is selfed the acquisition of 3 generations and stablizes homozygous lines BC5F4, per generation is all single fringe harvest, these backcross progenies are passed through selfing
Separation offspring is generated, in conjunction with field natural high-temperature stress and phjytotron simulation high temperature stress identification, in advanced backcross analysis
The height that middle selection boot stage is heat-resisting, yielding ability is good carries out species test for Backcross introgression family, selects through boot stage through high temperature stress
After processing, setting percentage is higher than 10% or more recurrent parent, and comprehensive agronomy trait expression and recurrent parent consistent boot stage are heat-resisting
The new germ plasm that property significantly improves.
Further, the offspring obtained after backcrossing is first selfed, and screens the good stable single plant of comprehensive agronomy character, so
It is selfed again afterwards, while carrying out high temperature stress screening.
Further, the offspring obtained after backcrossing first carries out in selfing step, and the number of selfing is 2 times or more, and preferably 2
It is secondary.Selecting for two generations is that the good stable single plant of comprehensive agronomy character carries out selfing progress high temperature stress.
Further, the high temperature stress was carried out in the booting high temperature sensitive phase, and the high temperature stress time is 3-5 days.
Further, first 3 days of high temperature stress time coincide with pollen mother cell meiosis.
In the present invention, the booting high temperature sensitive phase is pollen mother cell meiosis and microspore early stage, heading stage
Preceding the 16-19 days.
Further, the number for being selfed while carrying out high temperature stress is 2 times or more, preferably 3 times.In the step, selfing
Carry out high temperature stress in the watery stage afterwards, the stable single plant of screening high temperature stress character is as final rice chalky grain rate high temperature insensitiveness
Germplasm.
Further, the high temperature stress is using in phjytotron simulation high temperature stress, field natural high-temperature stress
It is any one or more.
The number of selfing is 3 times, can be such as the item of simulating high temperature stress in selfing process twice using phjytotron
Part is screened in the environment of natural high-temperature stress.
Further, the condition of the phjytotron simulation high temperature stress are as follows: early 8 points are not less than to late 5 temperature
38.0 ± 0.5 DEG C, remaining time is 25.0 ± 0.5 DEG C.
Further, the field natural high-temperature stress are as follows: mean daily temperature is not less than 32 DEG C, and maximum temperature is not less than 36
℃。
Further, also set up following parameter: relative humidity is 75 ± 5%, light application time 14h, and intensity of illumination is
20000~25 000lx.
In the present invention, the precise Identification of boot stage Holstein Cattle by following methods as a result, obtained: will be female thin in pollen
Born of the same parents' differential period and growing way it is consistent height for Backcross introgression system plant and each 20 plants of recurrent parent (being transplanted in basin), in booting high temperature
The artificial climate of sensitive periods (pollen mother cell meiosis and microspore early stage, 16-19 days before heading stage) continuous 4d of progress
Room simulation heat evil Stress treatment, and the preceding 3d of processing is made to coincide with pollen mother cell meiosis.High temperature stress processing
Daytime (38.0 ± 0.5) DEG C and night (28.0 ± 0.5) DEG C, light application time 14h, relative humidity are set to controlled temperature
75% ± 5%, daylight intensity is 20000~25 000lx.After processing, test material is moved on under room temperature, until mature.
Setting percentage is investigated, each processing is all provided with control (control is each test material under field normal temperature growth conditions), with final single
Fringe is averaged setting percentage variation as metrics evaluation boot stage heat resistance.
Further, lower than receptor setting percentage 10% or more strain is selected after each high temperature stress processing.
The good backcross progeny strain of breeding comprehensive agronomy character is selfed, and is carried out at high temperature stress while selfing
Reason reappears boot stage setting percentage performance after high temperature stress and is higher than 10% or more recurrent parent setting percentage in selfing process
Stable strain；Specific choice standard is combined from generation to generation to BC5F4: 1. in booting high temperature sensitive phase manual simulation's high temperature stress following table
Now increase by 10% or more than recurrent parent setting percentage for setting percentage；2. 115.0 centimetres of plant height or so；3. 24.0 centimetres of spike length with
On；4. Defined daily doses 90.0 or more, averagely increasing by 15.0 or more than recurrent parent；5. effective fringe of single plant 9 or more.Into
Row list fringe sowing, adds for homozygosis separation strain, obtains target strain until stablizing.
Further, the long-grained nonglutinous rice includes that Yang No.6 rice, China account for (SC2-S6 surveys extensive system and selects).
Preferably, the heat-resisting rice germplasm system is Oryza glaberrima Steud resource IRGC102309.
Oryza glaberrima Steud IRGC102309 is the cultivation rice varieties from West Africa, be limited to West Africa Sahara Desert with
The area plantation in south, has the characteristics that good drought-resistant, high temperature, is the valuable source for being worth utilizing, but its yield, quality
Etc. characters it is poor, directly using breed breeding purpose is not achieved.But it is controlled excellent by building height for Backcross introgression system
The genetic locus of character, which penetrates into long-grained nonglutinous rice elite restorer lines, can effectively overcome its disadvantage.The present invention is hybridized using primary, and 5 times times
Hand over, be selfed three times effectively break it is unfavorable chain, in conjunction with phjytotron simulate high temperature stress and field natural high-temperature stress identification,
It selects setting percentage under high temperature stress and is higher than 10% or more recurrent parent, the comprehensive agronomies such as yield and quality character and recurrent parent base
The new material that this consistent boot stage heat resistance significantly improves.
Further, the rice variety is Yang No.6 rice.
Compared with prior art, the invention has the benefit that
(1) the existing heat-resisting genetic resources of boot stage heat resistant variety are mostly derived from long-grained nonglutinous rice, tropic Japonica and part Asia
Common wild-rice, the present invention excavate to obtain boot stage heat-resisting new germ plasm from Oryza glaberrima Steud, have widened boot stage and heat-resisting have educated
The hereditary basis of kind.
(2) method that the present invention was returned, is repeatedly selfed using high generation, breaks and boot stage Holstein Cattle and low yield, poor quality
Etc. unfavorable gene linkage, homozygosis is carried out to boot stage heat-resisting gene in advanced lines and is fixed, genetic expression can be stablized.
(3) particularly, invention is combined using Oryza glaberrima Steud IRGC102309 and Yang No.6 rice as material by high generation backcrossing
Phjytotron carries out continuous 4 days heat evil Stress treatments (coinciding with pollen mother cell meiosis for first 3 days), and selection obtains
The new germ plasm that boot stage heat resistance significantly improves was obtained, enables the boot stage of Oryza glaberrima Steud IRGC102309 heat-resisting gene
It is used for long-grained nonglutinous rice breeding practice, all has highly important meaning in science and production.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described.
Fig. 1 is the rice restorer boot stage heat-resisting germplasm selection flow chart provided in the embodiment of the present invention.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
As shown in Figure 1, the heat-resisting new germ plasm selection of a kind of Rise's boot period provided by the invention, long-grained nonglutinous rice is with indica conventional rice
Kind Yang No.6 rice is receptor parent, and Oryza glaberrima Steud boot stage heat-resisting germplasm line IRGC102309 is donor parents, hybridizes for 1 generation,
It was returned for 5 generations, obtains BC5F1 seed.Plantation is primary, obtains BC5F2 seed, selects 100 plants of good single plants of comprehensive agronomy character
It is selfed bagging sowing.Other Main Agronomic Characters and economic characters specific choice standard are as follows: 1. within 145 days breeding times；2. plant height
115.0 centimetres or so；3. 24.0 centimetres of spike length or more；4. Defined daily doses 170 or more；5. effective fringe of single plant 9 or more；⑥
30 grams of mass of 1000 kernel.Field management carries out according to a conventional method.
These backcross progenies (BC5F3, BC5F4 and BC5F5) are obtained by 3 generations of selfing and stablize homozygous lines BC5F5, often
Generation is all single fringe harvest.In selfing process, a field is combined certainly after identifying using the simulation high temperature stress of phjytotron twice
Right high temperature stress identification, selects the height that boot stage is heat-resisting, yielding ability is good to be examined for Backcross introgression system in advanced backcross analysis
Kind, it selects boot stage heat resistance and is significantly higher than recurrent parent, comprehensive agronomy character and recurrent parent consistent boot stage are heat-resisting
New germ plasm.
Citing is carried out below to be described in detail.
Embodiment 1
1.1 test sites: super rice research and development central laboratory of academy of agricultural sciences of Jiangxi Province and Nanchang proving ground.
1.2 materials and method
1.2.1 material
Using middle Xian conventional variety Yang No.6 rice (as two-line hybrid rice backbone parent also known as 9311) for recurrent parent, with non-
Continent cultivated rice IRGC102309 is donor parents (nonrecurrent parent).
1.2.2 method is summarized
Using the boot stage heat-resisting gene money of the methods of primary hybridization, five backcrossings, five selfings transformation Oryza glaberrima Steud
Source.Hybrid parent, backcrossing and inbreeding population combine plantation using field interval sowing and potting.Potted plant is mainly used for people
Work climatic chamber identifies boot stage heat resistance, and field interval sowing is mainly used for the identification of boot stage natural high-temperature stress and comprehensive agronomy
Character investigation.On the basis of field, phjytotron identification, comprehensive agronomy character observation knot is combined using objective trait selection
Fruit, which determines, is promoted to strain and final election strain, specifically: its yield, plant type, breeding time etc. are selected with field, and the interiors such as rice matter are examined
Kind, rice quality is measured using laboratory equipment.In the segregating populations menu fringe harvest such as hybridization early period, backcrossing, selfing；Later period is steady
Grouping body is harvested by row and investigates comprehensive agronomy character.
The present invention hybridizes the heat-resisting germplasm line IRGC102309 of Oryza glaberrima Steud and Yang No.6 rice (9311), carries out single
Fringe selection, and phjytotron is combined to carry out High Temperature Simulation stress identification boot stage heat resistance, 2 phase of 2010 fraction of the year is sowed and transplants
It is grown in basin alms bowl.Phjytotron is moved on in sensitive periods in boot stage (pollen mother cell meiosis), carries out daytime continuous 4
(high temperature stress processing and controlled temperature are set to (38.0 ± 0.5) DEG C and (25.0 ± 0.5) for its 38 DEG C of high temperature stress identification
DEG C, light application time 14h, relative humidity 75% ± 5%, daylight intensity is 20000~25 000lx.).After treatment,
It is mature to move to natural conditions normal growth.Setting percentage is investigated after 30-45 days, it is heat-resisting as boot stage using final single fringe setting percentage that is averaged
The evaluation index of property.Final phenotype investigation result, Oryza glaberrima Steud after discovery boot stage experience high temperature stress processing
IRGC102309 (Philippine's International Rice is provided) setting percentage is 68.3% and 64.9%, Yang No.6 rice (Lixiahe District respectively
The indica type conventional rice of the incubations such as regional Institute of agricultural sciences Zhang Hongxi) setting percentage be 29.8% and 30.3%.Above two
A germplasm materials disclose free granting, generally utilize in rice in China genetic breeding unit.If other colleagues need simultaneously,
Jiangxi Province super rice research and development center can provide these germplasm to studies in China unit.
1.3 test procedure
1.3.1 hybridization
Summer in 2011 Nanchang experiment station of Jiangxi Academy of Agricultural Sciences plant respectively Oryza glaberrima Steud IRGC102309 and
Each a line of Yang No.6 rice, Yang No.6 rice are female parent, and IRGC102309 is that the combination of male parent preparing hybrid generates F1, are all mixed after mature
It receives.
1.3.2 backcross progeny selects
Winter in 2011 plants 2 row F1,8 plants of every row in Sanya, Hainan breeding base, and backcrossing obtains BC1F1 seed, mature
It is all mixed afterwards to receive.Summer in 2012 Jiangxi Academy of Agricultural Sciences Nanchang proving ground (24 plants) of BC1F1 group of plantation continue with
Recurrent parent is returned to obtain BC2F1 seed.Winter in 2012 is in Jiangxi Academy of Agricultural Sciences's Sanya, Hainan breeding base kind BC2F1
Group's (50 plants) selects 10 plants at random and is returned with recurrent parent 9311, and mixed receipts obtain BC3F1 seed.Summer in 2013 is in Jiangxi
BC3F1 group (80 plants) are planted in Shanxi Academy of Agricultural Sciences Nanchang proving ground, select 20 plants at random and continue to be returned, mixed receipts obtain
BC4F1 seed, winter Jiangxi Academy of Agricultural Sciences's Sanya, Hainan breeding base kind BC4F1 group (200 plants), selects 30 plants at random
Continue to be returned with recurrent parent, mixed receipts obtain BC5F1 seed.Summer in 2014 is in Jiangxi Academy of Agricultural Sciences Nanchang proving ground
500 plants of BC5F1 group is planted, single plant is collected to obtain BC5F2 seed；Continue to plant, single plant is collected to obtain BC5F3 seed.Select 2
In secondary selfing, 100 plants of comprehensive agronomy characters show good single plant sowing.Other Main Agronomic Characters and economic characters specific choice
Standard is as follows: 1. within 145 days breeding times；2. 115.0 centimetres of plant height or so；3. 24.0 centimetres of spike length or more；4. every fringe reality grain
Number 170 or more；5. effective fringe of single plant 9 or more；6. 30 grams of mass of 1000 kernel.Field management carries out according to a conventional method.
1.3.3 first time phjytotron simulation high temperature stress selection
Summer in 2015 plants the self progeny group of above-mentioned 100 single plants in Nanchang experiment station of Jiangxi Academy of Agricultural Sciences
(BC5F3), 40 plants of each strain kind, while (9311) 20 plants of recurrent parent are planted, the main tiller to 10% looks into young fringe about 0.5 through stripping
Centimetre or so when, in the case where not hurting root system as far as possible, band mud move into basin alms bowl in continued growth.In main tiller ear differentiation to close
When grain husk flower half length (pollen mother cell meiosis), by the backcrossing single plant and recurrent parent almost the same in growing way
Tiller is listed, and each strain at least guarantees there is 15 plants of listed, dated strain numbers, listed date on board.According to listed plant
The growing process speed of strain tiller is different, is moved into artificial climate room in batches and carries out the heat evil Stress treatment of continuous 4d.Simultaneously really
Protecting makes the preceding 3d of processing coincide with pollen mother cell meiosis.High temperature stress processing and controlled temperature are set to
(38.0 ± 0.5) DEG C and (25.0 ± 0.5) DEG C, light application time 14h, relative humidity 75% ± 5%, intensity of illumination is 20000~
25 000lx.After treatment moves to restoration ecosystem under normal natural conditions, and listed single fringe setting percentage is investigated after 35-40 days, with
The average setting percentage of listed list fringe is that boot stage Evaluation of Heat Tolerance index is investigated.It rejects Some Agronomic Traits and is in separation shape
The strain of state identifies altogether 6 boot stages setting percentage after high temperature stress and is higher than 10% or more recurrent parent setting percentage
Stable strain.
1.3.3 second of phjytotron simulation high temperature stress selection
After summer in 2016 continues to plant in Nanchang experiment station of Jiangxi Academy of Agricultural Sciences the selfing of above-mentioned 6 middle roguing systems
For group (BC5F4), 50 plants of each strain kind, while planting 30 plants of recurrent parent (Yang No.6 rice).Such as artificial gas of above-mentioned first time
It waits room and simulates high temperature stress identification method.When 10% main tiller looks into about 0.5 centimetre of young fringe or so through stripping, do not hurting root system as far as possible
In the case where, band mud moves into continued growth in basin alms bowl.In main tiller ear differentiation to (pollen mother cell when spending half length close to grain husk
Meiophase), the tiller in the consistent backcross population single plant of growing way and recurrent parent is listed, and move into people in batches
The heat evil Stress treatment of continuous 4d is carried out in work climatic chamber.Ensure to make the preceding 3d and pollen mother cell meiosis of processing simultaneously
It coincides.High temperature stress processing and controlled temperature are set to (38.0 ± 0.5) DEG C and (25.0 ± 0.5) DEG C, light application time
14h, relative humidity 75% ± 5%, daylight intensity are 20000~25 000lx.After treatment moves to normal natural item
Restoration ecosystem under part investigates listed single fringe setting percentage after 35 days, using the average setting percentage of listed single fringe as Evaluation of Heat Tolerance index
It is investigated.Identify in total 5 can reappear boot stage after high temperature stress setting percentage be higher than recurrent parent setting percentage
10% or more stable strain.
1.3.4 third time field natural high-temperature stress selection
Summer in 2017 continues in Nanchang experiment station of Jiangxi Academy of Agricultural Sciences to high through second of phjytotron simulation
5 strains (BC5F5) that temperature coerces selection carry out continuous 5 batches of interval sowings (being specifically shown in Table 1), rice transplanting in 30 days after broadcasting.Each
Middle roguing is each issue kind 50 plants.Every part of material sets 3 repetitions, conventional water and fertilizer management.Record field in July 1 to 20 period of August
Between maximum temperature, mean daily temperature.Wherein July -28 days on the 17th, occurring within continuous three days 33.0 DEG C of field mean temperature or more has four
It is secondary, and the highest temperature is more than 38.0 DEG C, is specifically shown in Table 2.
1 interval sowing table of table
| Sow issue | The first phase | The second phase | The third phase | The fourth phase | The fifth phase |
| Sowing date | April 10 | April 17 | April 24 | May 1 | May 8 |
2 field mean daily temperature of table and maximum temperature investigation result
| Investigate the date | Mean daily temperature | Maximum temperature |
| Sowing date | 33℃ | 38℃ |
| July 17 | 34℃ | 39℃ |
| July 18 | 33℃ | 38℃ |
| July 19 | 32℃ | 37℃ |
| July 20 | 34℃ | 39℃ |
| July 21 | 32℃ | 36℃ |
| July 22 | 33℃ | 38℃ |
| July 23 | 34℃ | 39℃ |
| July 24 | 34℃ | 39℃ |
| July 25 | 31℃ | 37℃ |
| July 26 | 33℃ | 38℃ |
| July 27 | 33℃ | 38℃ |
| July 28 | 34℃ | 39℃ |
The tiller of 16-18 days (pollen mother cell meiosis) is listed after each issue of each material ear differentiation,
Young fringe and grain husk flower are about final length half at this time, and listed date, strain number are indicated on board.Listed single plant knot is investigated after maturation
Real rate and other Main Agronomic Characters, each period is all provided with control, and (control parent is to raise rice 6 under the normal growing conditions of field
Number).Investigation meiophase successively goes through the setting percentage of the listed fringe of high temperature stress after ripening in 3 days.By middle roguing system setting percentage
Evaluation heading flowering period Heat-tolerant index is used as with the difference for compareing parent's setting percentage.Wherein the second phase, the third phase, the fourth phase are broadcast
Kind material has listed fringe to meet the high temperature stress weather in the period on the -28th July 17 in pollen mother cell meiosis
(appearance occurs 33.0 DEG C of field mean temperature or more for three days on end, and the highest temperature is more than 38.0 DEG C).
The listed specific investigation result of fringe setting percentage such as the following table 3.
Listed fringe setting percentage and Other Main Agronomic Characters performance under 3 field natural high-temperature of table stress
**Indicate 0.01 level of signifiance
By variance analysis, select simulation in the controlled environment chamber high in above 5 pure lines identified as can be seen from Table 2
Setting percentage heat resistance is higher than recurrent parent by 10% or more (p < 0.01) in the case of temperature stress and field high temperature stress, and main agriculture
Skill character and economic characters are all satisfied standard chosen below: 1. within 145 days breeding times；2. 115.0 centimetres of plant height or so；3. fringe
It is 24.0 centimetres long or more；4. Defined daily doses 170 or more；5. effective fringe of single plant 9 or more；6. 30 grams of mass of 1000 kernel or so.
The heat-resisting strain background parent that the present invention selects is middle Xian conventional variety Yang No.6 rice (as two-line hybrid rice backbone
9311) and China two is the maximum restorer of indica hybrid rice application area parent also known as, therefore pass through the selection
The new lines of breeding are that indica hybrid rice boot stage heat enduring vegetables breeding provides new germ plasm resource for two, grind in subsequent science
Study carefully and production application in have a very important significance.
In addition, the Yang No.6 rice in embodiment 1, which is replaced with China, accounts for (SC2-S6 surveys extensive system and selects), remain to so effective that obtain water
Rice chalky grain rate high temperature insensitiveness germplasm.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention
Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of heat-resisting germplasm selection of Rise's boot period, which comprises the following steps:
Long-grained nonglutinous rice is hybridized as donor as receptor, heat-resisting rice germplasm system, obtains F1 generation；
F1 generation and receptor are returned 4-6 times, obtain BCnF1 generation；
In BCnF1 generation, is selfed, and while selfing, high temperature stress is carried out, until heat-resisting kind of the boot stage for obtaining stabilization characteristics of genetics
Matter.
2. selection according to claim 1, which is characterized in that the offspring obtained after backcrossing is first selfed, screening
The good stable single plant of comprehensive agronomy character, is then selfed, while carrying out high temperature stress screening again.
3. selection according to claim 2, which is characterized in that the offspring obtained after backcrossing first carries out selfing step
In, the number of selfing is 2 times or more, preferably 2 times.
4. selection according to claim 1, which is characterized in that the high temperature stress be sensitive periods in boot stage into
Row, high temperature stress time are 3-5 days；
Further, first 3 days of high temperature stress time coincide with pollen mother cell meiosis.
5. selection according to claim 4, which is characterized in that the number for being selfed while carrying out high temperature stress is 2 times
More than, preferably 3 times.
6. selection according to claim 1, which is characterized in that the high temperature stress is high using phjytotron simulation
Temperature stress, field natural high-temperature stress are any one or more of；
Further, the condition of the phjytotron simulation high temperature stress are as follows: early 8 points are not less than 38.0 to 5 temperature of evening
± 0.5 DEG C, remaining time is 25.0 ± 0.5 DEG C；
Further, the field natural high-temperature stress are as follows: mean daily temperature is not less than 32 DEG C, and maximum temperature is not less than 36 DEG C.
7. selection according to claim 6, which is characterized in that during the high temperature stress, also set up following ginseng
Number: relative humidity is 75 ± 5%, light application time 14h, and intensity of illumination is 20000~25 000lx.
8. selection according to claim 1, which is characterized in that selection is more solid than receptor after each high temperature stress processing
The strain of rate low 10% or more.
9. selection according to claim 1-8, which is characterized in that the long-grained nonglutinous rice includes that Yang No.6 rice, China account for
(SC2-S6 surveys extensive system's choosing)；
Preferably, the heat-resisting rice germplasm system is Oryza glaberrima Steud resource IRGC102309.
10. selection according to claim 9, which is characterized in that the long-grained nonglutinous rice is Yang No.6 rice.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110205402A (en) * | 2019-07-03 | 2019-09-06 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Identification of early-flowering trait QTL of rice and application of early-flowering trait QTL in rice breeding |
| CN112161916A (en) * | 2020-09-30 | 2021-01-01 | 四川省农业科学院水稻高粱研究所 | Identification and evaluation method of high-temperature drought-resistant rice variety |
| CN112369319A (en) * | 2020-11-12 | 2021-02-19 | 安徽荃银高科种业股份有限公司 | Method for breeding high-quality rice variety in high temperature period based on alkali elimination value |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417001A (en) * | 2016-09-28 | 2017-02-22 | 云南省农业科学院生物技术与种质资源研究所 | Breeding method of Indica rice variety resistant to cold in booting and flowering stages |
| CN107006366A (en) * | 2017-02-27 | 2017-08-04 | 湖北省农业科学院粮食作物研究所 | A kind of method that big mass screening cultivates high temperature resistance paddy rice |
-
2018
- 2018-09-29 CN CN201811146035.0A patent/CN108925423B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417001A (en) * | 2016-09-28 | 2017-02-22 | 云南省农业科学院生物技术与种质资源研究所 | Breeding method of Indica rice variety resistant to cold in booting and flowering stages |
| CN107006366A (en) * | 2017-02-27 | 2017-08-04 | 湖北省农业科学院粮食作物研究所 | A kind of method that big mass screening cultivates high temperature resistance paddy rice |
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| CN110205402B (en) * | 2019-07-03 | 2020-07-03 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Identification of early-flowering trait QTL of rice and application of early-flowering trait QTL in rice breeding |
| CN112161916A (en) * | 2020-09-30 | 2021-01-01 | 四川省农业科学院水稻高粱研究所 | Identification and evaluation method of high-temperature drought-resistant rice variety |
| CN112369319A (en) * | 2020-11-12 | 2021-02-19 | 安徽荃银高科种业股份有限公司 | Method for breeding high-quality rice variety in high temperature period based on alkali elimination value |
| CN112369319B (en) * | 2020-11-12 | 2023-10-03 | 安徽荃银高科种业股份有限公司 | Method for breeding high-quality rice varieties in high temperature period based on alkali extinction value |
| CN113728828A (en) * | 2021-06-16 | 2021-12-03 | 袁隆平农业高科技股份有限公司 | Method for identifying heat resistance and cold resistance of rice |
| CN114521459A (en) * | 2022-02-22 | 2022-05-24 | 安徽国瑞种业有限公司 | Method for cultivating heat-resistant disease-resistant nitrogen efficient rice |
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