CN104221844A - Method for cultivating low-thermo-sensitive type two-line male sterile line core seed - Google Patents
Method for cultivating low-thermo-sensitive type two-line male sterile line core seed Download PDFInfo
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- CN104221844A CN104221844A CN201410473628.3A CN201410473628A CN104221844A CN 104221844 A CN104221844 A CN 104221844A CN 201410473628 A CN201410473628 A CN 201410473628A CN 104221844 A CN104221844 A CN 104221844A
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Abstract
The invention provides a method for cultivating a core seed with stable male sterility. The cultivated seed is high in outcrossing rate, high in combining ability and short in growth period. The method comprises the steps as follows: a two-line male sterile line sterile gene is selected, and a novel two-line male sterile line is obtained through crossbreeding; a filial generation of a three-line sterile maintenance line and a high-quality conventional variety is selected and hybridized with the two-line sterile line to perform the genetic recombination ; a selfed seed with sterile line and conventional variety interaction genes is selected through the filial generation; according to filial generation F1 plant separation characteristics, various types of sterile lines are continuously selected, and the fertility of the two-line male sterile line is gradually stabilized; different types of restoring lines are selected to perform test crossing with the different types of two-line sterile lines respectively, and hybrid vigor is observed; and a strongly-optimal two-line sterile line single plant is screened based on observation of the hybrid vigor, and the low-thermo-sensitive type sterile line core seed is screened out under the action of the natural pressure according to the special geographic positions and climate conditions of hill fields of the cold highland area.
Description
Technical field
Present inventor relates to rice breeding method, is specifically related to the breeding method that a kind of two are male sterile line core seeds.
Background technology
Current two is that male sterile line kind utilizes male sterile line can exempt artificial emasculation, save manpower in production application; Be compare minimizing production link with three, reduce seed produces cost and labour intensity, ensure seed purity, thus TwoLine Method hybrid rice variety selection earns widespread respect in breeding field; Particularly two is that male sterile line can free combo with conventional variety in breed breeding, so both widen seed selection scope, expand seed selection thinking again, improve hybrid vigour level, can select and produce the upper all kinds Cross Combinations required, be the development trend that crop hybrid superiority utilizes.
TwoLine Method sterile line breeding be by between two kinds cytoplasm core mutually based on two be the kind of male sterile line and conventional variety and the effective way of use of advantage.One excellent two is that male sterile line must possess stable fertility, and coordinate force is strong, and flowering habit is excellent, and sword-like leave length is shorter, resistance and the feature such as rice is of fine quality.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of low-temperature-sensitive two to be the breeding method of male sterile line core seeds, the seed cultivated possesses male sterility and stablizes, outcrossing seed-setting rate is high, coordinate force is strong, breeding time is short, not only can reach two and be heterosis, hybrid vigor but also can reduce production cost, finally realize the object of increasing both production and income in production application.
For solving the problems of the technologies described above, low-temperature-sensitive two of the present invention is the breeding method of male sterile line core seeds, comprises the following steps:
Step 1: be male sterile line " Y58S " for maternal with two, account for F6 for male parent with middle 9B/ Guangdong perfume (or spice) and hybridize, plantation crossbreed of obtaining, after ripe, mixed receipts crossbreed offspring is F1;
Step 2: plantation filial generation F1, is separated the excellent sterile strain of selection traits strain from offspring after maturation, cutting root and stem of certain plants regeneration results self-fertility single-strain seed is F2;
Step 3: plantation self-fertility single-strain seed F2, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F3;
Step 4: plant sterile strain F3, selects excellent sterile strains to carry out cutting the root and stem of certain plants after maturation from offspring is separated, and results self-fertility seed is F4;
Step 5: plantation self-fertility single-strain seed F4, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F5;
Step 6: plant sterile strain F5, selects excellent sterile strains to carry out cutting the root and stem of certain plants after maturation from offspring is separated, and results self-fertility seed is F6;
Step 7: plantation self-fertility single-strain seed F6, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F7;
Step 8: plant sterile strain F7, selects excellent sterile strains to carry out cutting the root and stem of certain plants after maturation from offspring is separated, and results self-fertility seed is F8;
Step 9: plantation self-fertility single-strain seed F8, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F9; Gene is basicly stable, economical character reaches unanimity;
Step 10: plantation F9, chooses proterties excellent sterile individual plant and carries out cutting the root and stem of certain plants from Progeny Lines Derived garden, and plant division results self-fertility seed is F10;
Step 11: the F10 selecting receipts in high altitude localities plantation respectively, under natural pressure condition, selects low-temperature-sensitive two to be male sterile line core seeds F11.This step utilizes two to be that the feature that male sterile line recovers fertility under cryogenic carries out natural selection, and selected regional height above sea level should higher than 600 meters.
After have employed technique scheme, give full play to heterosis, hybrid vigor, gene optimization is utilized to recombinate and artificially breeding, unified with nature pressure regulates to be selected, and the low-temperature-sensitive two of cultivation is that male sterile line core seeds possesses sterility and stablizes, and Fertility Transfer Temperature is low, the excellent outcrossing seed-setting rate of strain Leaf pattern, Spike granule character is high, coordinate force is strong, the features such as breeding time is short, and in production application, not only can reach two is that heterosis, hybrid vigor utilizes but also can reduce production cost, finally realizes the object of increasing both production and income.
Described step 2,4,6, implemented in China In Middle And Lower Reaches of Changjiang River in 8 positive seasons, described step 3,5,7,9 winters implemented in China Hainan Region.Its objective is and utilize each department natural conditions to accelerate cultivation process.
In described step 10, it is F10 ' that all the other individual plants not selected cut the root and stem of certain plants mixed receipts self-fertility seed.Can be used for test cross, also can be directly used in the production of hybrid seeds.
Embodiment
The object of the invention is to cultivate the stable core seeds of a kind of male sterility, origin temp is low, and outcrossing seed-setting rate is high, and coordinate force is strong, breeding time is short, and in production application, not only can reach two is that heterosis, hybrid vigor utilizes but also can reduce production cost, finally realizes the object of increasing both production and income.
General thought of the present invention
(1) select two to be male sterile line sterile gene by warm photocontrol, by crossbreeding means, obtaining new two is male sterile line.
(2) three are selected to be sterile maintainer and high-quality conventional variety filial generation by being the effect that male sterile line Crossing system reaches genetic recombination with two.
(3) selfed seed with male sterile line and conventional variety interacting genes is selected by filial generation.
(4) plant stalling characteristic by filial generation F1 kind, constantly select various types of male sterile line, and make two to be that male sterile line fertility is stablized gradually.
(5) select dissimilar restorer to be that male sterile line carries out test cross respectively with dissimilar two, observe hybrid vigour.
(6) excellent two be male sterile line individual plant by force by observing hybrid vigour screening, recycling high and cold mountain area Shan Pang field Special geographical position and weather conditions filter out low thermo-sensitive type caryon sterile line core seeds under the effect of natural pressure.
(7) be that male sterile line core seeds and existing superior restorer line 9311 etc. carry out test cross by two again and to select two of stable fertility be gradually sterile strain.
Seed selection route of the present invention
1, low-temperature-sensitive two of the present invention is male sterile line core seeds is with " Y58S " for maternal, and it is that male parent carries out selection cross that middle 9B/ Guangdong perfume accounts for F6.Y58S is a kind of ripe two male sterile line kinds being, possesses plant type more satisfactory, and tillering ability is strong, the feature that rice quality is excellent, but also also exist that fringe type is less, sword-like leave length grows (affecting outcrossing), weakness that thousand kernel weight is lower partially.Male parent is selected and has been used three to be that sterile maintainer " middle 9B and high-quality conventional variety Guangdong perfume (or spice) account for " carries out filial generation F6 seed (middle 9B/ Guangdong perfume (or spice) accounts for F6) aborning.
2, this sterile to lie in First Year positive season be maternal in Anqing Area, China middle and lower reach of Yangtze River with " Y58S " is that male parent is hybridized with the fragrant intermediate materials accounting for F6 in 9B/ Guangdong in high-quality precocity, autumn gathers in the crops crossbreed.
3, winter, crossbreed was planted in Hainan then, Second Year spring mixed receipts (Y58S//middle 9B/ Guangdong perfume (or spice) accounts for) crossbreed Offspring F1 seed.
4, plantation Y58S//middle 9B/ Guangdong perfume (or spice) accounts for and filial generation F1 seed positive season plants 4800 strains, and autumn is separated the excellent sterile strain of selection traits strain 32 individual plants from offspring, and cutting root and stem of certain plants regeneration results 32 self-fertility single-strain seeds is F2.
5, plantation Y58S//middle 9B/ Guangdong perfume (or spice) accounts for filial generation F2 sterile strain self-fertility seed and plants 32 strains, and from its offspring is separated, the sterile strain of all kinds 38 individual plants are received in choosing is F3.
6, plant Y58S//middle 9B/ Guangdong perfume (or spice) and account for filial generation 38 F3 individual plants, from offspring is separated, selects excellent sterile strains 21 individual plants to carry out cutting the root and stem of certain plants, results self-fertility seed is F4.
7, so positive season Anqing, two places, Hainan in winter come and go accumulative 5 years, its offspring F9 is basicly stable, and economical character reaches unanimity, and wherein 2 strains are had outstanding performance.
8, excellent sterile strains 2 single-strain seeds of filial generation F9 are accounted for by plantation Y58S//middle 9B/ Guangdong perfume, from Progeny Lines Derived garden, choose 95 excellent sterile individual plants carry out cutting the root and stem of certain plants, plant division results self-fertility seed F10, all the other individual plants cut the root and stem of certain plants mixed receipts self-fertility seed F10 '.
9, low-temperature-sensitive two is selected to be male sterile line core seeds F11.Planted 95 the fine individual plant self-fertility seed F10 selecting receipts respectively by high altitude localities, under natural pressure condition, select low thermo-sensitive type core seeds to be F11.
10, meanwhile mixing receipts two in other local plantations is male-sterile seed F10 ', is mainly used to carry out hybridization respectively from different Elite restorer lines and surveys excellent, and gather in the crops hybrid seed.
11, combo odds ratio comparatively.To cultivate after terminating the ground plantation such as in Anqing to be used for surveying excellent hybrid seed and to carry out varietal yield test, performance selected two is that male sterile line combo offspring is with the obvious advantage.
12, the low-temperature-sensitive two cultivated is that male sterile line core seeds F11 is by field and climatic cabinate qualification.
13, the low-temperature-sensitive two prepared by the present invention is male sterile line core seeds can called after two be male sterile line N708S, its title is originated: the self-fertility seed F10 of receipts is selected in plantation respectively, heading stage one by one individual plant pollen by microscopic examination, finally select the individual plant that is numbered N708.
Botany morphological characters
The indica type low-temperature-sensitive dual purpose genic male sterile line of this product kind.Duration from seeding to heading 70-82 days, average 72.6 days; Plant height 80.0cm; Plant type is moderate, and blade is recessed and upright, and leaf look dark green; Leaf sheath, bran point are colourless; Leaf number of the main culm 14.3; Tillering ability is strong, and the percentage of earbearing tiller is high, the effective fringe of individual plant 10.0; About spike length 20.4-22.1cm, total grain panicle number 160-204 grain, the long particle shape of grain, thousand kernel weight 25 grams; Colony's regularity is good; Sterile plant rate 100%, pollen sterility degree 100%, pollen sterile loses based on allusion quotation.Stigma exposure rate about 70%, stigma exposing ratio is high.
Test cross advantage
Be male sterile line core seeds F11 and multiple free restorer test cross with low-temperature-sensitive two, all show the features such as the ripe phase is moderate, and ripening rate is good, rice matter is good.
First excellent 828 (N708S/R828) of pioneer combination N two joined for female parent with low thermo-sensitive type core seeds F11, N Liangyou108 (N708S/R108), N two excellent 746 all show the features such as output is high, rice is of fine quality.
Low thermo-sensitive type core seeds " N708S " and former two prepared by the present invention is that male sterile line " Y58S " is distinguished
" N708S " and former two is that male sterile line " Y58S " compares: 1, strain Leaf pattern: " N708S " blade is wide, and sword-like leave is short, and plant is high, and leaf look green, is conducive to male sterile line pollination, can improves ripening rate in production application.2, Spike granule character: " N708S " spike length is short, grain close, many 30 ~ 50 of grains per panicle.3, breeding time: " N708S " breeding time is short 3 ~ 4 days.
Resistance
Low thermo-sensitive type core seeds " N708S " strain Leaf pattern performance prepared by the present invention has stronger lodging resistance and stronger cold resistance, meets its ripening rate of high temperature apparently higher than other male sterile lines season, certain high-temperature resistance is described in the Two-line system hybrid rice production of hybrid seeds.
Claims (3)
1. low-temperature-sensitive two is a breeding method for male sterile line core seeds, comprises the following steps:
Step 1: be male sterile line " Y58S " for maternal with two, account for F6 for male parent with middle 9B/ Guangdong perfume (or spice) and hybridize, plantation crossbreed of obtaining, after ripe, mixed receipts crossbreed offspring is F1;
Step 2: plantation filial generation F1, is separated the excellent sterile strain of selection traits strain from offspring after maturation, cutting root and stem of certain plants regeneration results self-fertility single-strain seed is F2;
Step 3: plantation self-fertility single-strain seed F2, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F3;
Step 4: plant sterile strain F3, selects excellent sterile strains to carry out cutting the root and stem of certain plants after maturation from offspring is separated, and results self-fertility seed is F4;
Step 5: plantation self-fertility single-strain seed F4, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F5;
Step 6: plant sterile strain F5, selects excellent sterile strains to carry out cutting the root and stem of certain plants after maturation from offspring is separated, and results self-fertility seed is F6;
Step 7: plantation self-fertility single-strain seed F6, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F7;
Step 8: plant sterile strain F7, selects excellent sterile strains to carry out cutting the root and stem of certain plants after maturation from offspring is separated, and results self-fertility seed is F8;
Step 9: plantation self-fertility single-strain seed F8, after maturation, from its offspring is separated, individual plant choosing receives all kinds picture like sterile strain is F9; Gene is basicly stable, economical character reaches unanimity;
Step 10: plantation F9, chooses proterties excellent sterile individual plant and carries out cutting the root and stem of certain plants from Progeny Lines Derived garden, and plant division results self-fertility seed is F10;
Step 11: the F10 selecting receipts in high altitude localities plantation respectively, under natural pressure condition, selects low-temperature-sensitive two to be male sterile line core seeds F11.
2. low-temperature-sensitive two as claimed in claim 1 is the breeding method of male sterile line core seeds, it is characterized in that: described step 2,4,6, implemented in China In Middle And Lower Reaches of Changjiang River in 8 positive seasons, described step 3,5,7,9 winters implemented in China Hainan Region.
3. low-temperature-sensitive two as claimed in claim 1 is the breeding method of male sterile line core seeds, it is characterized in that: in described step 10, and it is F10 ' that all the other individual plants not selected cut the root and stem of certain plants mixed receipts self-fertility seed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410473628.3A CN104221844A (en) | 2014-09-17 | 2014-09-17 | Method for cultivating low-thermo-sensitive type two-line male sterile line core seed |
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| CN201410473628.3A CN104221844A (en) | 2014-09-17 | 2014-09-17 | Method for cultivating low-thermo-sensitive type two-line male sterile line core seed |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145334A (en) * | 2015-07-10 | 2015-12-16 | 江苏省徐州大华种业有限公司 | Seed selection method of new rice species |
| CN105432459A (en) * | 2015-12-08 | 2016-03-30 | 安徽中科安岳林业科技发展有限公司 | Cultivation method of low temperature sensitive two-line male-sterile core seeds |
| CN105638446A (en) * | 2016-03-16 | 2016-06-08 | 湖南省水稻研究所 | Method for breeding rice dual-purpose genic male sterile line with excellent seed production characteristics |
| CN106857233A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of two is the breeding method of Black rice sterile line |
| CN106942046A (en) * | 2017-03-22 | 2017-07-14 | 湖南杂交水稻研究中心 | A kind of two be the breeding method of Glutinous CMS line |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145334A (en) * | 2015-07-10 | 2015-12-16 | 江苏省徐州大华种业有限公司 | Seed selection method of new rice species |
| CN105432459A (en) * | 2015-12-08 | 2016-03-30 | 安徽中科安岳林业科技发展有限公司 | Cultivation method of low temperature sensitive two-line male-sterile core seeds |
| CN105638446A (en) * | 2016-03-16 | 2016-06-08 | 湖南省水稻研究所 | Method for breeding rice dual-purpose genic male sterile line with excellent seed production characteristics |
| CN106857233A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of two is the breeding method of Black rice sterile line |
| CN106942046A (en) * | 2017-03-22 | 2017-07-14 | 湖南杂交水稻研究中心 | A kind of two be the breeding method of Glutinous CMS line |
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Application publication date: 20141224 |