CN106942046A - A kind of two be the breeding method of Glutinous CMS line - Google Patents
A kind of two be the breeding method of Glutinous CMS line Download PDFInfo
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- CN106942046A CN106942046A CN201710172012.6A CN201710172012A CN106942046A CN 106942046 A CN106942046 A CN 106942046A CN 201710172012 A CN201710172012 A CN 201710172012A CN 106942046 A CN106942046 A CN 106942046A
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- glutinous
- seed
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- glutinous rice
- sterile
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the breeding method that a kind of two are Glutinous CMS line, comprise the following steps：The glutinous rice conventional variety of collection and excellent two-line sterile line are hybridized, F1 generation seed is harvested to plant in Changsha, in hybridization F2 generations, are initially separated, complete sterile plant is selected under the conditions of megathermal climate, take the sterile plant standing grain root and stem of certain plants to Hainan in Winter breeding, the solid generation seed of sterile line, selection glutinous rice seed is simultaneously planted it in Changsha, the complete sterile plant of progeny selection is separated under the conditions of megathermal climate, take the sterile plant standing grain root and stem of certain plants to Hainan in Winter breeding, seed selection is circulated by 68 generations, stable glutinous rice two-line sterile line is cultivated；Fertility identification is carried out in the controlled environment chamber, is proceeded by 23 DEG C of low-temperature treatments in ear differentiation fourth stage, is selected and cultivate low temperature sensitive sterile strain；Combo and test cross are carried out by the other excellent glutinous rice varieties different from other genetic background, is evaluated by F1 generation Heterosis, the glutinous rice Hybrid Rice Varieties of high yield, quality height and better resistance can be screened.
Description
Technical field
Field is cultivated the present invention relates to glutinous rice variety, especially a kind of two be the breeding method of Glutinous CMS line.
Background technology
Glutinous rice belongs to Asian Cultivated Rice（Oryza sativa L.）, it is with a long history, it is widely used, germ plasm resource is enriched.It is glutinous
The characteristics of rice be starch in endosperm based on amylopectin, up to 95-100%, amylose content is extremely low（＜ 2.0%）.Because
With gel consistence is soft, viscosity big, the features such as be difficult retrogradation, glutinous rice turns into Zongzi, glutinous rice cake, mixed congee, various sweets and brewages Huang
The required raw material of all multi-products such as wine, sweet rice wine, glutinous rice is rich in protein and fat, and nutritive value is higher.In recent years, people's people's livelihood
The flat raising of running water proposes new demand to the nutrition and health care function of food, and the quickening of rhythm of life is for convenience of rice and quick-frozen
The development of the novel foodstuffs such as food creates new space.Therefore, the expansion of the market demand and the change of consumption structure are educated to glutinous rice
Plant and indicate new direction, it is proposed that new demand.
Waxy endosperm is single recessive gene in the first linkage group by rice chromosome（wxwx）Controlled, positioned at water
On the 6th chromosome of rice, meet mendelian inheritance.Waxy genewx The synthesis of starch, influence food directly in control seed
Flavor quality and grain yield.China's glutinous rice is widely distributed, and each main equal seed selection of rice region meets local geographical, market environment needs
Glutinous rice variety.North paddyfield is based on round-grained rice type glutinous rice, generally late rice, relatively resists cold drought-enduring；Southern glutinous rice Xian round-grained rice has, kind
The glutinous rice variety that wide variety, the especially middle and lower reach of Yangtze River and Fujian are bred as is more, and yield is higher；Yunnan, Guizhou and Shan Xihan
Middle area is special due to geographical and humanistic environment, aboundresources, including much extraordinary rice, but because geographical environment is limited output typically
Not high, other characteristic traits protruded are cold-resistant, not resistant to lodging for shade tolerant.The seed selection of special and extraordinary glutinous rice concentrates on THE LOWER YANGTZE VALLEY
Area, and with diversity and specific aim, such as wine rice, the giant embryo glutinous rice of health care colored rice and the multiple merits of aggregation, perfume (or spice)
Glutinous rice etc..In production using glutinous rice variety based on conventional variety, waxy hybrid rice kind is fresh to see less, utilizes hybrid rice skill
Art, can improve effective fringe and number of grain per ear, improving quality and raising yield.This patent selection cross technology, cultivation is produced
Measure that high, best in quality, pest and disease damage better resistance and breeding time be adapted to two are glutinous rice hybrid rice parentses sterile line, pass through the miscellaneous of combo
Glutinous rice is handed over to go out stronger hybrid vigour in yield traits and other trait expressions.
The content of the invention
In view of this, it is an object of the invention to provide the breeding method that a kind of two are Glutinous CMS line, production can be screened
The waxy hybrid rice kind that amount height, quality height, better resistance and breeding time are adapted to.
The technical scheme is that：A kind of two be the breeding method of Glutinous CMS line, is comprised the following steps：
（1）By the high-quality gathered, routinely the glutinous two-line sterile line with excellent spread hybridizes, the kind that harvest F1 generation is tied
Son；
（2）By step（1）Obtained F1 generation seed is planted under the weather of Changsha, and the hybridization F2 generations planted out start to separate,
The sterile plant for having separation is found under the conditions of megathermal climate, complete sterile plant is selected；
（3）By step（2）The winter breeding that the obtained sterile plant standing grain root and stem of certain plants is taken under the weather conditions of Hainan, the solid production of the sterile plant standing grain root and stem of certain plants
Non-hibernating eggs, opens after glume after being dried with Wagner's reagent identification or seed and identifies, selects the glutinous rice identified
Seed；
（4）By step（3）Obtained glutinous rice seed is planted under the weather conditions of Changsha, and offspring's choosing is separated under the conditions of megathermal climate
Complete sterile plant is selected, then the winter that the sterile plant standing grain root and stem of certain plants is taken under the weather conditions of Hainan breeds, and circulates seed selection by 6-8 generations, no
Educate strain and start stabilization, cultivate stable glutinous rice two-line sterile line；
（5）Fertility identification is carried out under the weather conditions of Changsha, under 23 DEG C of low temperature conditions, is proceeded by ear differentiation fourth stage
Low-temperature treatment, selects and cultivates low temperature sensitive sterile strain；
（6）By step（5）Obtained low temperature sensitive sterile strain other excellent glutinous rice varieties different from other genetic background are carried out
Combo and test cross, are evaluated by F1 generation Heterosis, can screen high rice yield, quality height, better resistance and breeding time suitable
The Hybrid Rice Varieties of conjunction.
Preferably, the step（4）Middle glutinous rice two-line sterile line is that plant type is excellent, breeding time is adapted to and pest and disease damage better resistance
Glutinous rice two-line sterile line.
Compared with prior art, the present invention has the advantages that：One kind two that the present invention is provided is Glutinous CMS line
Breeding method, solve that conventional glutinous rice variety plant type is bad, yield poorly, pest and disease damage resistance and the problems such as poor quality, thus may be used
To screen the waxy hybrid rice kind that high rice yield, quality height, better resistance and breeding time are adapted to.
Brief description of the drawings
Fig. 1 is flow chart of the invention.
Embodiment
Below in conjunction with the accompanying drawings and embodiment, the present invention will be further described.
As shown in figure 1, a kind of two be the breeding method of Glutinous CMS line, comprise the following steps：
（1）By the high-quality gathered, routinely the glutinous two-line sterile line with excellent spread hybridizes, the kind that harvest F1 generation is tied
Son；
（2）By step（1）Obtained F1 generation seed is planted under the weather of Changsha, is separated since F2 generations, in megathermal climate
Under the conditions of find there is the sterile plant of separation, select and identify complete sterile plant；
（3）By step（2）The winter breeding that the obtained complete sterile plant standing grain root and stem of certain plants is taken under the weather conditions of Hainan, sterile plant standing grain root and stem of certain plants knot
Seed is produced in fact, is peeled off after glume and is identified after being dried with Wagner's reagent identification or seed, it is glutinous that selection is identified
Rice；
（4）By step（3）Obtained glutinous rice seed is planted under the weather conditions of Changsha, and offspring's choosing is separated under the conditions of megathermal climate
Complete sterile plant is selected, then the winter that the sterile plant standing grain root and stem of certain plants is taken under the weather conditions of Hainan breeds, by 6-8 for seed selection, strain is opened
Begin stable, cultivate excellent plant type, pest and disease damage better resistance, breeding time suitable glutinous rice two-line sterile line；
（5）Fertility identification is carried out under the weather conditions of Changsha, under 23 DEG C of low temperature conditions, is proceeded by ear differentiation fourth stage
Low-temperature treatment, selects and cultivates low temperature sensitive sterile strain；
（6）By step（5）Obtained low temperature sensitive sterile strain other excellent glutinous rice varieties different from other genetic background are carried out
Combo and test cross, are evaluated by F1 generation Heterosis, can screen high rice yield, quality height, better resistance and breeding time suitable
The Hybrid Rice Varieties of conjunction.
The present invention provide a kind of two be Glutinous CMS line breeding method, solve conventional glutinous rice variety plant type it is bad,
Yield poorly, pest and disease damage resistance and the problems such as poor quality, it is possible thereby to screen, yield is high, quality is high, better resistance and breeding time are adapted to
Waxy hybrid rice kind, two be that Glutinous CMS line reproductive output reaches 2-3 tons/hectare, and glutinous rice Rice Hybrid Seed Production reaches 3-4
Ton/hectare, two be that glutinous rice hybrid paddy rice establishing in large scale can reach 8-10 tons/hectare, and conventional glutinous rice variety be higher by 2-3 tons/
The resistance of hectare, two-line sterile line rice blast resistance and brown paddy plant hopper reaches more than moderate resistance.
The above embodiments are only the preferred embodiments of the present invention, it is impossible to the interest field of the present invention is limited with this, because
This, modification, equivalent variations, improvement for being made according to scope of the present invention patent etc. still belong to the scope that the present invention is covered.
Claims (2)
1. a kind of two be the breeding method of Glutinous CMS line, it is characterised in that is comprised the following steps：
By the high-quality gathered, routinely the glutinous two-line sterile line with excellent spread hybridizes, the seed that harvest F1 generation is tied；
By step（1）Obtained F1 generation seed plantation harvest F2 plants F2 for seed, in the maturity period for seed under the weather of Changsha
It can be seen that being separated since F2 generations, the complete sterile plant of separation is found under the conditions of megathermal climate, and mark completely not
Educate strain；
By step（2）The winter breeding that the obtained complete sterile plant standing grain root and stem of certain plants is taken under the weather conditions of Hainan, the sterile plant standing grain root and stem of certain plants is solid
Seed is produced, peels off after glume and identifies after being dried with Wagner's reagent identification or seed, the glutinous rice identified is selected
Seed；
By step（3）Obtained glutinous rice seed is planted under the weather conditions of Changsha, and progeny selection is separated under the conditions of megathermal climate
Complete sterile plant, then the winter that the sterile plant standing grain root and stem of certain plants is taken under the weather conditions of Hainan breed, by 6-8 generation circulation seed selections, infertility
Strain starts stabilization, and it is Glutinous CMS line to cultivate stable two；
Fertility identification is carried out under the weather conditions of Changsha, under 23 DEG C of low temperature conditions, is proceeded by ear differentiation fourth stage low
Temperature processing, selects and cultivates low-temperature-sensitive Glutinous CMS line；
By step（5）Obtained low-temperature-sensitive Glutinous CMS line other excellent glutinous rice varieties different from other genetic background（It is extensive
Multiple system）Combo and test cross are carried out, is evaluated in field by F1 generation Heterosis, can be screened than the production of conventional glutinous rice variety
Measure the double-line hybrid glutinous rice variety that high, best in quality, pest and disease damage better resistance and breeding time are adapted to.
2. according to claim 1 a kind of two be the breeding method of Glutinous CMS line, it is characterised in that the step（4）
In two be Glutinous CMS line for plant type is excellent, breeding time is adapted to, the two of pest and disease damage better resistance be Glutinous CMS line.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710172012.6A CN106942046A (en) | 2017-03-22 | 2017-03-22 | A kind of two be the breeding method of Glutinous CMS line |
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| CN201710172012.6A CN106942046A (en) | 2017-03-22 | 2017-03-22 | A kind of two be the breeding method of Glutinous CMS line |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857233A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of two is the breeding method of Black rice sterile line |
| CN111543314A (en) * | 2020-06-29 | 2020-08-18 | 四川发生种业有限责任公司 | Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line |
| CN111837939A (en) * | 2020-07-30 | 2020-10-30 | 吴烽 | Method for fixing rice heterosis |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
| CN104221844A (en) * | 2014-09-17 | 2014-12-24 | 安徽宇顺种业开发有限公司 | Method for cultivating low-thermo-sensitive type two-line male sterile line core seed |
| CN104823839A (en) * | 2015-05-22 | 2015-08-12 | 福建农林大学 | Breeding method of anthocyanin-rich black rice thermo-sensitive two-line sterile line |
| CN105432459A (en) * | 2015-12-08 | 2016-03-30 | 安徽中科安岳林业科技发展有限公司 | Cultivation method of low temperature sensitive two-line male-sterile core seeds |
| CN105638446A (en) * | 2016-03-16 | 2016-06-08 | 湖南省水稻研究所 | Method for breeding rice dual-purpose genic male sterile line with excellent seed production characteristics |
-
2017
- 2017-03-22 CN CN201710172012.6A patent/CN106942046A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
| CN104221844A (en) * | 2014-09-17 | 2014-12-24 | 安徽宇顺种业开发有限公司 | Method for cultivating low-thermo-sensitive type two-line male sterile line core seed |
| CN104823839A (en) * | 2015-05-22 | 2015-08-12 | 福建农林大学 | Breeding method of anthocyanin-rich black rice thermo-sensitive two-line sterile line |
| CN105432459A (en) * | 2015-12-08 | 2016-03-30 | 安徽中科安岳林业科技发展有限公司 | Cultivation method of low temperature sensitive two-line male-sterile core seeds |
| CN105638446A (en) * | 2016-03-16 | 2016-06-08 | 湖南省水稻研究所 | Method for breeding rice dual-purpose genic male sterile line with excellent seed production characteristics |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857233A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of two is the breeding method of Black rice sterile line |
| CN111543314A (en) * | 2020-06-29 | 2020-08-18 | 四川发生种业有限责任公司 | Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line |
| CN111837939A (en) * | 2020-07-30 | 2020-10-30 | 吴烽 | Method for fixing rice heterosis |
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Application publication date: 20170714 |