CN112075338A - Breeding method of multi-cryptosweet waxy corn inbred line - Google Patents
Breeding method of multi-cryptosweet waxy corn inbred line Download PDFInfo
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Abstract
The invention discloses a breeding method of a multiple-recessive-sweet-waxy maize inbred line, which is characterized in that the existing sweet-waxy double-recessive (bt)1bt1Based on wxwx) corn germplasm resources, different types of sweet-waxy double-hidden (su) are further collected and introduced1su1wxwx)、(sh2sh2wxwx) gene corn germplasm resource, performs group material construction, amplification and improvement innovation between sweet and waxy double-hidden materials, and realizes the purpose of (bt)1bt1wxwx) type sweet and glutinous double-hidden selfing line and hybrid rice containing (sh)2sh2su1su1wxwx) sweet and waxy hybrid seeds are used as basic materials, a conventional pedigree method is combined with molecular marker-assisted directional selection, and through selfing generation-adding selection, testing preparation and evaluation, the novel multi-recessive homozygous sweet and waxy corn material is cultivated.
Description
Technical Field
The invention belongs to the technical field of new crop variety breeding, and particularly relates to a breeding method of a multiple-cryptosweet waxy corn inbred line.
Background
At present, China is in the leading position of the world in the breeding aspect of waxy and sweet waxy corn varieties. Waxy corn originates from the southwest region of China and is formed by recessive mutation of wx gene in common corn, while sweet waxy corn is another original fresh-eating corn variety type which is independently innovated after the original domesticated waxy corn in China, and has the characteristics of high sugar content, strong palatability, rich nutritional ingredients necessary for human body and the like, and the sweet waxy corn has short growth period and high added value, can meet different market demands of animal husbandry development and resident dietary structure change, has very high economic value and application prospect, and is one of the main directions of the breeding research of fresh-eating corn at present.
The endosperm is one of the important components of the corn kernel, is a place for accumulating and storing nutrient substances, and the improvement of the nutrient quality of the corn kernel mainly focuses on endosperm mutation variants. Therefore, the research on corn mutants is mainly focused on endosperm mutants, most of the corn endosperm mutants are inherited by single recessive genes, most of the genetic effects of the mutants only act on endosperm, more than 20 of currently discovered corn endosperm mutants are available, and the corn endosperm mutants mainly have su with certain or potential application value1、sh2、ae、wx、o2、su2、bt1、bt2Se, etc. For endosperm su, sh2、bt2Wx gene expression is modified by genetic background to different degrees, and when two or more pairs of recessive genes are in a homozygous state, the genes interact with each other to generate a new phenotype. Various recessive endosperm mutant genes have specific gene effects. Different types of double-cryptic and multiple-cryptic gene mutants can further improve the structure of endosperm, compared with single-cryptic gene mutantsThe endosperm of the gene mutant has the defects in the aspects of quality and agronomic characters, and the nutritional quality of the corn can be improved by utilizing the endosperm mutation recessive gene interaction effect. The endosperm gene interaction is applied to the aspects of special corn breeding, improving the breeding efficiency, improving the corn kernel nutritional quality and the like, and becomes an important means for corn breeding.
The domestic research on the sweet and waxy corn variety mainly focuses on the research on the sweet and waxy double-hidden corn material, and the quality of the sweet and waxy corn is controlled by sweet and waxy 2-class genes. At present, most of sweet waxy corn varieties in the market are made of super sweet waxy corn (wx/wx, sh)2/sh2) The sweet and glutinous corn variety bred by mutual parent hybridization between the double-recessive-gene inbred line and the glutinous corn (wx/wx) single-recessive-gene inbred line, i.e. one of the parents contains both sweet and glutinous double-recessive genes (sh)2sh2wxwx), followed by hybridization with another parent waxy species (wxwx), F1The kernel is waxy (Sh)2sh2wxwx)，F2The cluster contains sweet grains and glutinous grains, the sweetness of the cluster is determined by the sweet gene in the kernel endosperm, the glutinousness of the cluster is determined by the content of amylopectin in the kernel endosperm, the ratio of the sweet grains to the glutinous grains is 1:3, the sweet grains and the glutinous grains are expressed on the same cluster, and the eating mouth feel shows the mouth feel quality of both sweet and glutinous.
Disclosure of Invention
The invention aims to provide a catalyst containing wx and bt1、sh2And su1A breeding method of a genetic multi-cryptosweet waxy corn inbred line.
The invention solves the technical problems by the following technical scheme:
a breeding method of a multi-cryptosweet waxy corn inbred line comprises the following steps:
(1) get (bt)1bt1wxwx) genotype sweet glutinous hybrid, planting F1Isolating and pollinating to obtain F2Generation, at F2Selecting the cluster with sweet grains, selecting white sweet grains, and continuously isolating and selfing strong excellent plants for 7 generations (bt)1bt1wxwx) genotype sweet-waxy double crypto-bicyclic system;
(2) obtaining (su)1su1wxwx) genotype hybrids and (sh)2sh2wxwx) genotype hybrid, and open-type free mixing to obtain the product containing (sh)2sh2su1su1wxwx) progeny of a sweet waxy maize hybrid of the three recessive genes;
(3) with strong excellent strain (bt)1bt1wxwx) genotype crisp sweet waxy double crypto bicyclic line as female parent, contains (sh)2sh2su1su1wxwx) three recessive genes as male parent to obtain wx and bt1、sh2And su1The multiple recessive corn comprehensive hybrid seeds are obtained;
(4) planting the hybrid ears obtained above in an isolation area, mixing and pollinating for one time, and performing isolation pollination on the obtained hybrid ears F2Selecting the ears with sweet grains in the generations, selecting white sweet grains, performing directional selection by means of a pedigree method in combination with molecular markers, and isolating and continuously selfing and pollinating for 8 generations through selfing generation-adding selection, testing and matching and evaluation, so as to culture a stable multi-recessive homozygotic sweet waxy corn selfing line, which is numbered bsw 9941.
The following further defined or optimized technical solutions are adopted:
said (bt)1bt1wxwx) the genotype sweet and glutinous hybrid is crisp, sweet and glutinous No. 6; said (su)1su1wxwx) genotype hybrid is sweet and glutinous; said (sh)2sh2wxwx) genotype hybrids are suave-yang sweet waxy and sweet-waxy 88.
The pedigree method is used for continuous selfing to breed a stable maize selfing line and is carried out by combining the pedigree method with a molecular marker-assisted directional selection method.
The corn inbred line bsw9941 is hybridized with waxy single recessive genotype corn to obtain F1The sweet and waxy corn has waxy kernel and is planted separately2The two kinds of grains, namely sweet grains and glutinous grains, are arranged on the same cluster, and the number ratio of the sweet grains to the glutinous grains is 1: 1.
The waxy single recessive genotype is waxy (wxwx).
The maize inbred linebsw9941 is hybridized with sweet single recessive or sweet glutinous double recessive genotype corn to obtain F1The grains of the sweet corn are sweet.
The sweet mono-recessive genotype corn comprises prasusweet (su)1su1) Type, crisp sweet (btbt) type and super sweet (sh)2sh2) And (4) molding.
The sweet and glutinous double recessive genotype corn is crisp, sweet and glutinous double recessive (bt)1bt1wxwx) type, super-sweet and glutinous double-hidden (sh)2sh2wxwx) and ordinary sweet and glutinous double-hidden (su)1su1wxwx).
Compared with the prior art, the invention achieves the following technical progress:
(1) the maize inbred line bsw9941 obtained by the invention is sweet, the kernel in the milk stage is milky white, and the top of the kernel in the full stage is reddish brown (figure 4).
(2) The corn inbred line bsw9941 obtained by the invention is hybridized with the recessive waxy wx type corn inbred line to obtain the first filial generation sweet and waxy corn, F1The grains are glutinous and planted separately F2The sweet and glutinous rice has sweet and glutinous grains on the same cluster, the number ratio of the sweet grains to the glutinous grains is 1:1 (figure 2), and the eating mouth feel shows sweet and glutinous quality.
(3) The maize inbred line bsw9941 obtained by the invention is detected by a conventional method (figure 3), bsw9941 contains wx and su1、bt1And sh2The maize inbred line with multiple recessive genes.
(4) The corn inbred line bsw9941 obtained by the invention and prasuan (su)1su1) Type, crisp sweet (btbt) type and super sweet (sh)2sh2) Hybridizing the type-identical single recessive gene sweet corn to the first filial generation sweet corn, F1The grains are sweet, after isolated planting, F2The kernel is sweet, and the sweetness is not lower than that of the original single cryptic parent sweet corn (figure 3).
(5) The corn inbred line bsw9941 obtained by the invention is combined with crisp, sweet and glutinous double-hidden (bt)1bt1wxwx) type, super-sweet and glutinous double-hidden (sh)2sh2wxwx) and ordinary sweet and glutinous double-hidden (su)1su1wxwx) type iso-sweet and waxy double recessive gene corn hybridFirst generation hybrid sweet corn, F1The grains are sweet, after isolated planting, F2The seeds are sweet, and the sweetness is not lower than that of the original parent sweet-waxy double-hidden corn (figure 3).
Drawings
FIG. 1 is a spectrogram of a breeding line of the multi-cryptosweet waxy corn inbred line bsw 9941.
FIG. 2 shows the F-shaped cross-section of the female parent poly-cryptosweet waxy corn inbred line bsw9941 and waxy corn inbred line2And (5) a dry fruit ear replacing graph.
FIG. 3 is a diagram of conventional identification and testing of the female parent poly-cryptosweet waxy maize inbred line bsw9941 in accordance with the present invention.
Fig. 4 is a diagram of dry ears of a multi-cryptosweet waxy corn inbred line bsw9941 in the milk stage and the complete stage.
FIG. 5 is the selection breeding spectrum of the female parent multiple cryptosweet waxy corn inbred line bsw9941 in the embodiment of the invention.
FIG. 6 shows the super sweet and glutinous rice of the male parent and the double hidden parent (sh) in the embodiment of the present invention2sh2wxwx) genotype maize inbred line sw2805 breeding line spectrogram.
FIG. 7 shows the male parent's natural sweet and glutinous rice's double-hidden (su) in the embodiment of the present invention1su1wxwx) genotype maize inbred line feng 1868 breeding line spectrogram.
FIG. 8 shows that the male parent is crisp, sweet and glutinous and has two recessive properties (bt)1bt1wxwx) genotype maize inbred line bw 1992.
FIG. 9 shows the "Tiancheng Xueya 207" variety approval certificate bred by using the multiple implicit sweet waxy corn inbred line bsw9941 as the parent strain.
FIG. 10 shows an embodiment sw2805 (sh) of the present invention2sh2wxwx type) is a 'Tiancheng fragrant sweet glutinous 262' variety approval certificate for parent breeding.
FIG. 11 shows 1868 (su) according to an embodiment of the present invention1su1wxwx type) as a parental breeding 'Tiancheng fragrant, sweet and glutinous' variety approval certificate.
FIG. 12 shows an embodiment bw1992 of the present invention (bt)1bt1wxwx type) is a 'Tiancheng glutinous 261' variety approval certificate for parent breeding.
Detailed Description
The concepts of the present solution will be described below using terms commonly employed by those skilled in the art to convey the substance of their work to others skilled in the art. The concepts may, however, be embodied in many different forms and the invention should not be construed as limited to the embodiments set forth herein. Specific embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of what is included to those skilled in the art. The particular embodiments shown and described may be substituted for a wide variety of alternate and/or equivalent implementations without departing from the scope of the present solution embodiments. This disclosure is intended to cover any adaptations or variations of the embodiments discussed in this disclosure. It will be apparent to those skilled in the art that alternative embodiments may be practiced using only some of the aspects of the described aspects. For purposes of illustration, specific parameters or values are set forth in the examples, however, one skilled in the art could, in light of the present disclosure, practice alternative examples by modifying these specific parameters or values. In other instances, well-known features may be omitted or simplified in order not to obscure the illustrative embodiments.
Example 1 Breeding of Polycryptosweet waxy maize inbred line bsw9941
As shown in fig. 1 and 5, the breeding method of the multiple cryptosweet waxy corn inbred line bsw9941 comprises the following steps: the method comprises the steps of obtaining a crisp, sweet and glutinous No. 6 hybrid seed in autumn in 2010, and planting F in spring in 2011 in the next year1Isolating pollination to obtain F2Generation, at F2Selecting the cluster with sweet grains in the generation, selecting white sweet grains for sowing, and selecting strong excellent plants (bt) in spring of 20141bt1wxwx) genotype crisp sweet glutinous double crypto bicyclic system as female parent, and sweet seed kernel obtained by open mixing of colorful sweet glutinous, Xinyang sweet glutinous and He sweet glutinous 882sh2su1su1wxwx) three recessive genes sweet waxy corn mixed seeds are used as male parents for hybridization, mixed pollination is carried out once in autumn through an isolation region in 2014, and F after the isolation pollination is carried out2Selecting the cluster with sweet seeds and selecting white sweetSeed planting, namely performing continuous bagging selfing by combining a pedigree method with molecular marker assisted directional selection after 2015 spring to form selfing S2→ selfing S3→ selfing S4→ selfing S5→ selfing S6→ selfing S7→ selfing S8Breeding stable corn inbred line bsw 9941.
As shown in FIG. 3, the obtained maize inbred line bsw9941 can be used as a parent to be hybridized with other maize with single recessive or double recessive genotype for application in agricultural production.
Example 2 Breeding of Tiancheng Xueyan 207 (examined No.: Guiju Yu 2020019) (FIG. 9) variety
1. Bsw9941 was used as the female parent.
2. Is made of super sweet and glutinous rice (sh)2sh2wxwx) genotype maize inbred line sw2805 as male parent. "Tiancheng fragrant and sweet glutinous 262" (Gui Ju Yu 2020047 number) male parent SW2805 is from Gui SW1338 and local excellent glutinous corn variety "glutinous heald 601" group comprehensive species (sh)2 sh2wx wx) genotype (fig. 10). The breeding method of sw2805 comprises the following steps: the comprehensive species of the sweet seed selected by the combination of the trial hybridization of the Guangxi district in 2013 and the local excellent waxy corn variety 'waxy comprehensive 601' is hybridized to be used as a basic material, and the mixture is planted and pollinated once through an isolation area, and F is selected from F2White sweet grains are selected and reserved from sweet grain clusters appearing in the generation for planting, and excellent plants are selected by a pedigree method for continuous selfing for 7 generations. The specific method comprises the following steps:
as shown in FIG. 6, the sweet seeds hybridized and combined in the autumn cinnamon selection SW1338 in 2013 and the comprehensive seeds of the corn glutinous rice combine 601 group are hybridized to form the base material, the hybrid ears are planted in the isolation area in 2014 for mixed pollination once, and strict plant selection and ear selection are carried out during harvesting to form the base material S0Planting the selected material in isolation area in autumn 2014, selecting plant before castration, bagging, isolating, and selfing to obtain S1Selecting excellent single plants as selected ears during harvesting, performing ear row sowing on the selected ears in 2015 spring, strictly selecting strong excellent plants for bagging and selfing to form selfing S2Self-bred S formed by continuous bagging selfing in 2015 autumn to 2017 spring3→ selfing S4→ selfing S5→ selfing S6→ selfing S7Breeding a waxy corn inbred line sw 2805.
And 3, breeding of Tiancheng xueyan 207: the male parent and the female parent are tested and matched with a hybrid combination in autumn of 2017, the multi-point variety ratio test is carried out on the test cross combination in spring and autumn of 2018, resistance, quality and yield are identified, a seedling combination sw2805 x bsw9941 with better comprehensive performance is screened according to spring data, and small-area seed production is carried out in autumn of 2018. A pure white sweet corn variety 'Tiancheng Xueyan 207' bred by hybridization selection is subjected to regional trial of a new Guangxi fresh waxy corn variety in 2019, and is already approved by Guangxi Zhuang autonomous region crop varieties.
Characteristic features of "Tiancheng Xueyan 207": pure white sweet corn, wherein the average period from emergence of seedlings to harvesting of fresh ears in spring is 77 days, the average period in autumn is 72 days, the plant type is flat, the first leaf sheath of the seedling is green, the tip of the first leaf is round to spoon-shaped, 17-20 leaves of the adult plant are formed, 18.3 leaves are averagely, the leaves are slender and sparse, the leaf color is green, the edge of the leaf is light green, and the character form degree of the stem is weak. The tassel has 15-18 branches at the first level, the bases of small flower glumes are green, the glume protection is green, the tips of the lemmas are green, the pollen amount is large, the anthers are green, the filaments are light green, the silks are tidy, the male and female parts are coordinated, and the penultimate leaves of the tassel grow to the 5 th-6 th leaves. The plant height is 217cm, the ear position is 79cm, the lodging rate is 4.6%, the turnover rate is 0.0%, the empty stalk rate is 1.2%, the double ear rate is 1.5%, the green retention degree is 89%, the tillering rate is 4%, the ear length is 18.6cm, the ear thickness is 4.8cm, the bald tip length is 0.6cm, the ear row number is 12-18 rows, the average row number is 15.7, the row number is 34, the hundred grain weight is 37.5g, the ear cylinder type is green, the bud leaves are provided with sword leaves, the ear stems are white, and the grains are white. The field records that the grade of the big spot is 1-7, the average grade is 1.3, the grade of the small spot is 1-3, the average grade is 1.5, the disease index of the sheath blight is 0.0-31.1, the average grade is 9.8, the bacterial wilt is 0.0-0.0%, the average grade is 0.0%, the grade of the stem rot is 0.0-5.0%, the average grade is 0.7%, the grade of the rust disease is 1-3, and the average grade is 1.7.
The yield performance of the test of the "Tiancheng Xueyan 207" in the new variety region is as follows: in 2019, the average fresh ear yield in spring is 699.2 kg/mu, the yield is increased by 12.2% compared with the control, the corn is positioned at the 20 th position of 23 sweet corn varieties, the inter-site variation coefficient is 14.26%, and the per mu yield range is 512.5-812.1 kg/mu. The number of the test participants is 6 in spring, and the yield is increased by comparison in total of 6 in spring, which accounts for 100% of the number of the test participants. In 2019, the average yield of fresh fruit ears in autumn is 823.4 kg/mu, the yield is increased by 10.5% compared with the contrast, 20 sweet corn varieties are positioned at the 9 th position, the inter-site variation coefficient is 16.68%, and the yield per mu range is 566.7-1026.3 kg/mu. The yield of the autumn ginseng is increased by 6 points compared with that of the control in 6 points, which accounts for 100 percent of the autumn ginseng. The average fresh cluster yield in two seasons is 761.3 kg/mu, 11.3% higher than the control, and the average fresh cluster yield is 16 th. 12 points are tested in spring and autumn, and the yield is increased by 12 points and 100 percent.
The quality performance of the test of the "Tiancheng Xueyan 207" in the new variety is as follows: the quality evaluation of the two seasons is summarized with the sensory quality of 25.3 points, the smell and the flavor of 14.5 points, the color of 5.9 points, the sweetness of 16.1 points, the tenderness of 8.1 points, the thickness of the skin of 16.1 points, the quality evaluation grade is summarized with 86.0 points, and the quality evaluation grade is second grade according to the sensory grade index of the regional test fresh corn of China.
Example 3 Breeding of "Tiancheng fragrant, sweet and glutinous 255" variety is participated in the regional test of Guangxi crop new variety in 2020
1. Polycryptic gene bsw9941 was used as female parent.
2. Using single hidden gene (wxwx) type waxy corn self-bred line as male parent, cross-breeding to obtain white sweet waxy corn variety "Tiancheng fragrant sweet waxy 255", making hybrid seed be sweet and waxy, F1The grains are glutinous and planted separately F2The method is characterized in that the same cluster has sweet and glutinous grains, the number ratio of the sweet grains to the glutinous grains is 1:1 (figure 2), and the method enters the district test of the new variety of fresh glutinous corns in Guangxi in 2020.
Example 4
1. Bsw9941 was used as the female parent.
2. As shown in FIG. 7, the two genes (su) of the two genes are selected from the group consisting of Punuo and nuo1su1wxwx) type corn inbred line Feng 1868 as male parent. "Tiancheng color sweet glutinous rice" (Gui Ju Yu 2020036 number) male parent "Feng 1868" is derived from "Feng color sweet glutinous rice" variety in Hebei province, and sweet glutinous rice is recessive1su1wxwx) genotype (fig. 10). The breeding method of Feng 1868 is as follows: the second cycle line is bred by selecting sweet grains through continuous selfing for 7 generations by using a colorful sweet and glutinous hybrid as a basic material. The specific method comprises the following steps:
feng 1868The two-implicit-two-cycle sweet and glutinous rice system is formed by taking rich and sweet and glutinous hybrid seeds introduced in Hebei as base materials and selecting excellent plants for continuous selfing for 7 generations in 2011. In autumn of 2011, the rich sweet glutinous hybrid introduced in Hebei is selected as the basic material for planting in the isolation region, and F is selected2Selecting sweet grains from the sweet waxy clusters, selfing and pollinating once in spring 2012, and strictly performing plant selection and cluster selection to form a base material S during harvesting1Planting the selected material in the isolation area in autumn of 2012, selecting plant before castration, bagging, isolating, and selfing to obtain S2Selecting excellent single plants as selected ears during harvesting, performing ear row sowing on the selected ears in spring of 2013, strictly selecting strong excellent plants, bagging and selfing to form selfing S3Self-bred S formed by continuous bagging selfing from 2016 spring to 2017 autumn4→ selfing S5→ selfing S6→ selfing S7The sweet and glutinous double-cryptomorphic inbred line Feng 1868 is bred.
3. As shown in FIG. 2, hybridization F1Grains appear sweet, F2The cluster is light yellow sweet corn, and the sweetness edible taste is higher than the original common sweet glutinous (su)1su1wxwxwx) type double cryptomaize parent inbred line "feng 1868".
4. The method comprises the steps of breeding natural color sweet waxy corn (combination name), testing and matching hybrid combination in autumn in 2017, carrying out multi-point quality ratio test on the test-crossing combination in spring and autumn in 2018, identifying resistance, quality and yield, screening a seedling combination with good comprehensive performance by using spring data, 1868 xzj 1928, carrying out small-area seed production in autumn in 2018, participating in a district test of a new variety of Guangxi fresh waxy corn in spring in 2019, and passing examination and determination in 2020.
Example 5
1. Bsw9941 was used as the female parent.
2. As shown in FIG. 8, the gene (bt) is encoded by a crisp, sweet and waxy double cryptic gene1bt1wxwx) type maize inbred line "bw 1992" as male parent. "Tiancheng glutinous 261" (Gui Ju Yu 2019027)' father parent "bw 1992", which is from Guangxi university college of farming Wu Zi Cai professor "crisp sweet glutinous No. 6", sweet glutinous double recessive is (bt)1bt1wxwx) genotype (fig. 11). The breeding method of the bw1992 is as follows: isolating and pollinating with a crisp, sweet and glutinous No. 6 hybrid, and performing pollination at F2Selecting ears with sweet grains, selecting white sweet grains, planting, and performing pedigree method for continuous selfing for 8 generations to obtain crisp sweet glutinous rice (bt)1bt1wxwx) type double recessive homozygote. The specific method comprises the following steps:
f after isolated pollination by using crisp, sweet and glutinous No. 6 hybrid seeds as basic material2Selecting sweet grains from the cluster, selecting white sweet grains, and performing continuous selfing for 8 generations by using pedigree method to obtain crisp sweet and glutinous double-hidden (bt)1bt1wxwx) type homozygote. The method comprises the steps of obtaining a crisp, sweet and glutinous No. 6 hybrid seed in autumn in 2010, and planting F in spring in 2011 in the next year1Inbred S obtained by isolating pollination1At S1Selecting ears with sweet grains in the generation, selecting white sweet grains in autumn in 2011, sowing, selecting strong excellent plants in autumn 2014, bagging and selfing continuously to form selfing S2→ selfing S3→ selfing S4→ selfing S5→ selfing S6→ selfing S7→ selfing S8And breeding a crisp, sweet and glutinous double recessive inbred line bw 1992.
3. As shown in FIG. 3, hybridization F1The grains are pure white sweet, F2The cluster is pure white sweet corn, and the sweetness of the cluster is not lower than that of the original crisp sweet glutinous (bt)1bt1wxwx) type double cryptic maize parent inbred line "bw 1992".
4. Breeding Tiancheng glutinous 261 (combination name), carrying out 2016 spring test hybridization combination, carrying out a multi-point sample ratio test on the test hybridization combination in 2016 autumn and 2017 spring, identifying resistance, quality and yield, screening a sprout combination with better comprehensive performance, carrying out small-area seed production in 2017 autumn, participating in a regional test on a new variety of Guangxi fresh glutinous corn in 2018 spring, and obtaining an approval in 2019.
Example 6
1. Bsw9941 was used as the female parent.
2. In super sweet (sh)2sh2) The type corn inbred line is taken as a male parent and is derived from Huazhen super-sweet corn.
3. As shown in FIG. 3, hybridization F1Grains appear sweet, F2The ears are yellow-white double-color sweet cornsThe sweetness of the product is not lower than the original super-sweet taste (sh)2sh2) A parent maize inbred line.
Example 7
1. Bsw9941 was used as the female parent.
2. Crisp sweet (btbt) corn variety 'crisp sweet Jade No. 1' (sweet corn hybrid variety from Guangdong province) is taken as a male parent.
3. As shown in FIG. 3, hybridization F1Grains appear sweet, F2The ears are yellow and white double-color sweet corns, and the sweetness of the ears is not lower than that of the original crisp and sweet (btbt) parent corn variety in eating mouthfeel.
Example 8
1. Bsw9941 was used as the female parent.
2. Is used to increase the sweetness of sugar (su)1su1) The type is derived from a maize inbred line of corn institute of Guangxi agricultural science institute as a male parent.
3. As shown in FIG. 3, hybridization F1Grains appear sweet, F2The cluster is yellow-white double-color sweet corn, and the sweetness of the cluster is higher than that of the original common sweet (su)1su1) A parent maize inbred line.
Claims (8)
1. A breeding method of a multi-cryptosweet waxy corn inbred line is characterized by comprising the following steps:
(1) get bt1bt1Planting Wxwx genotype sweet waxy corn hybrid F1Isolating and pollinating to obtain F2Generation, at F2Selecting the cluster with sweet grains in the generation, selecting white sweet grains, selecting strong excellent plants, continuously isolating, selfing and pollinating for 7 generations to obtain bt1bt1A wxwx genotype sweet and waxy double crypto second cycle system;
(2) obtaining su1su1wxwx and sh2sh2The wxwx genotype hybrid seeds are mixed with powder in an open and free way for one time to obtain the hybrid seeds containing sh2sh2su1su1Progeny of the sweet waxy corn mixed variety of the wxwx three recessive genes;
(3) by strong excellent strain bt1bt1wxwx genotype crisp, sweet and glutinous double-crypto bicyclic system asFemale parent of sh2sh2su1su1Sweet grains of the sweet waxy corn mixed species containing wxwx three recessive genes are used as male parents for hybridization to obtain the product containing wx and bt1、sh2And su1The multiple recessive corn comprehensive hybrid seeds are obtained;
(4) planting the hybrid ears obtained above in an isolation area, mixing and pollinating for one time, and performing isolation pollination on the obtained hybrid ears F2Selecting the ears with sweet grains in the generations, selecting white sweet grains, performing directional selection by means of a pedigree method in combination with molecular markers, and isolating and continuously selfing and pollinating for 8 generations through selfing generation-adding selection, testing and matching and evaluation, so as to culture a stable multi-recessive homozygotic sweet waxy corn selfing line, which is numbered bsw 9941.
2. The method for breeding the inbred line of poly-cryptosweet waxy corn according to claim 1, wherein bt is1bt1The wxwx genotype sweet and glutinous hybrid is crisp, sweet and glutinous No. 6; the su1su1The wxwx genotype hybrid is sweet and glutinous; the sh2sh2The wxwx genotype hybrids are Xinyang sweet glutinous and Heyang sweet glutinous 88.
3. The breeding method of the inbred line of the multi-cryptosweet waxy corn according to claim 1, characterized in that the stable inbred line of the corn is bred by the pedigree method through continuous inbred and the pedigree method is combined with a molecular marker assisted directional selection method.
4. The breeding method of the inbred line of multi-cryptosweet waxy corn of claim 1, wherein the inbred line bsw9941 is hybridized with waxy single recessive genotype corn to obtain F1The sweet and waxy corn has waxy kernel and is planted separately2The two kinds of grains, namely sweet grains and glutinous grains, are arranged on the same cluster, and the number ratio of the sweet grains to the glutinous grains is 1: 1.
5. The method for breeding the inbred lines of multi-cryptosweet waxy corn according to claim 1, wherein the inbred line of corn is bsw9941 and sweet single cryptosweet waxy cornHybridizing sexual or sweet and glutinous double recessive genotype corn to obtain F1The grains of the sweet corn are sweet.
6. The method for breeding the inbred line of multi-cryptosweet waxy corn according to claim 4, wherein the waxy single recessive genotype is waxy wx type.
7. The method for breeding the inbred line of multi-recessive-sweet-waxy-corn according to claim 5, wherein the sweet single-recessive-genotype corn is Pususu1su1Type, crisp sweet btbt type or super sweet sh2sh2)And (4) molding.
8. The breeding method of the inbred line of poly-cryptosweet-waxy corn of claim 5, wherein the sweet-waxy double-recessive genotype corn is crisp-sweet-waxy double-cryptobt1bt1wxwx type super-sweet and glutinous double-hidden sh2sh2wxwx type or common sweet and glutinous double-cryptosu1su1Type wxwx.
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