CN110301350A - A kind of selection of purple pagoda cauliflower cenospecies - Google Patents
A kind of selection of purple pagoda cauliflower cenospecies Download PDFInfo
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- CN110301350A CN110301350A CN201910741556.9A CN201910741556A CN110301350A CN 110301350 A CN110301350 A CN 110301350A CN 201910741556 A CN201910741556 A CN 201910741556A CN 110301350 A CN110301350 A CN 110301350A
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- purple
- cauliflower
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selections of purple pagoda cauliflower cenospecies, it is obtained using microspores culture or by traditional breeding method selfing homozygous purple DH system or inbred pure line as male parent, common pagoda cauliflower ogura cytoplasmic male sterile line mostly to obtain for backcross transformation obtains purple pagoda cauliflower first-filial generation new varieties by crossbreeding for female parent.The purple pagoda cauliflower of acquisition is to be formulated by ogura cytoplasmic male sterile line and purple cauliflower DH system, and recurrent parent is self-compatible, numerous system of cenospecies and sterile line can be carried out with honeybee pollination, seeding technique is reliable, substantially reduces breeding cost.
Description
Technical field
The invention belongs to field of plant breeding, and in particular to a kind of selection of purple pagoda cauliflower cenospecies.
Background technique
Pagoda cauliflower (Brassica oleracea L.var.botrytis cv.romanesco) also known as Rome flower coconut palm
Dish, bouquet have special external appearance characteristic, send out like Buddha's spiral shell hair worn in a bun or coil, arrange in the Pei Bonaqie ordered series of numbers point shape of rule, Mei Ge little
It is similar to big bouquet on bouquet.Pagoda cauliflower appears in Italy in 16th century, is Cruciferae Brassica genus brassica specie flower coconut palm
A kind of cauliflower variety type of dish mutation.Pagoda cauliflower last century Mo just start to plant in China, rich in vitamin C,
Vitamin K, crude fibre, mouthfeel is similar to general cauliflower, while ornamental value is preferable, as a kind of special vegetable by consumer gradually institute
It notes.
Pagoda cauliflower currently on the market is light green, and seed yet there are no purple pagoda from foreign countries both at home and abroad
The appearance or breeding research of cauliflower variety work.The germ plasm resource of common pagoda cauliflower is few, and is mostly sterility cross-bred
Kind, available resource is more rare.Therefore rich as can carrying out germplasm innovation and breeding utilization in few available resource
Rich pagoda cauliflower type, innovation are bred as not only nice but also good-looking new type, will meet market to the diversified need of variety type
It asks.
Applicant is on original pagoda cauliflower germplasm resource base, in conjunction with microspore culture and conventional hybridization breeding
Technology, using cytoplasmic male sterile line approach, having selected has independent intellectual property right, is fond of eating and has the purple of ornamental value again
Pagoda cauliflower cenospecies.
Summary of the invention
The purpose of the present invention is select purple pagoda cauliflower Hybrid with independent intellectual property rights.Utilize small spore
Sub- culture technique is quickly obtained homozygous breeding parent as recurrent parent or male parent, in conjunction with ogura cytoplasmic male sterility approach
Cenospecies female parent is selected, by hybrid seeding, Anthocyanin Content height is cultivated and tool ornamental value purple pagoda cauliflower is miscellaneous
Hand over generation new varieties.
The present invention is realized especially by following technical scheme:
A kind of selection of purple pagoda cauliflower cenospecies, comprising the following steps:
It 1) is female parent with the pagoda cauliflower Hybrid of the ogura cytoplasmic male sterility of introduction, with excellent double lists
Times system (DH system) is male parent, is hybridized.
2) transformation generation plant filters out the sterile single plant bloomed with Normal nectary, with self-compatible excellent DH system
For recurrent parent, carry out obtaining the ogura cytoplasmic male sterile line of inheritance stability mostly for backcross transformation.
3) bouquet circle and purple cauliflower germplasm are introduced, obtains F after being selfed for 1 generation2For segregating population, bouquet purple is screened
Deep, bouquet rounding single plant is separated again, obtains F3For strain, then to F3Microspores culture or selfing separation are carried out for plant,
Purple dihaploid homozygous plants are therefrom obtained, the excellent DH pure lines of bouquet purple depth are filtered out.
4) the ogura cytoplasmic male sterile line of the inheritance stability obtained with transformation is female parent, is obtained with microspores culture
Purple DH pure lines be male parent, preparing hybrid combination.
5) it is female parent that screening, which is obtained with the dihaploid sterile line, using dihaploid purple cauliflower as the hybridization of male parent
Kind, it is bred as excellent purple pagoda-shaped cauliflower cenospecies.
Further, ogura sterile source pagoda cauliflower material flower bud phase cabage-type rape hybridizes in step (1), obtains cell
A matter male transformation generation.
Further, after 6 generation of step (2) continuous backcross (BC6), the ogura cytoplasmic male sterility of inheritance stability is obtained
System makees maternal.
Further, with the F of purple cauliflower in step (3)3On behalf of microspore donor, the excellent of bouquet purple depth is filtered out
Good DH system makees male parent
The invention has the benefit that
1) initiative obtains purple pagoda cauliflower cenospecies, and bouquet purple, pagoda are pointed, and Comprehensive Traits performance in field is excellent
It is good, enrich cauliflower type.
2) compared with common pagoda-shaped cauliflower, the cenospecies bouquet purple, Anthocyanin Content is high, has novelty.
3) due to hybridizing the equal source Doubled haploid line of parents, cenospecies homozygosity is high, and the purple pagoda cauliflower of acquisition is miscellaneous
Kind of a neat and consistent is handed over, kind property is stablized.
4) the purple pagoda cauliflower obtained is to be formulated by ogura cytoplasmic male sterile line, and recurrent parent is
Self-compatible can carry out numerous system of cenospecies and sterile line with honeybee pollination, and seeding technique is reliable, substantially reduces breeding cost.
Detailed description of the invention
Fig. 1 is the breeding hybridized flow chart of the present invention.
Specific embodiment
Below in conjunction with specific embodiment of the present invention, technical solution of the present invention is clearly and completely described, is shown
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
As shown in Figure 1, present embodiments providing the selection of purple pagoda cauliflower cenospecies, following step is specifically included
It is rapid:
1) Screening of Germplasm and identification.Began in 2010, collected 7 portions of pagoda-shaped cauliflowers, began in 2013, collects
To 3 parts of purple cauliflower germ plasm resources.It is investigated through field planting, economical character and identification, filtering out can be 1 part of breeding utilization
Ogura cytoplasmic male sterility pagoda-shaped cauliflower and 3 portions of fertile pagoda-shaped cauliflowers and 2 parts of available bouquet consolidations
Spheroidal purple cauliflower germplasm.
2) male sterility source selects.Ogura cytoplasmic male sterility pagoda-shaped cauliflower cenospecies, is denoted as bj1841, table
Existing petal and nectary are normal, as male sterility source breeding material.
3) recurrent parent breeding.Using fertile leatherleaf pagoda-shaped cauliflower cenospecies as donor, it is denoted as RCK01, by small
Spore cultivation obtains 13 plants of dihaploid series, is denoted as DH1633, wherein DH1633-1 shows as leatherleaf, self-compatible, bouquet
Pale green, pagoda point, the development of florescence floral organ is normal, as sterile maternal recurrent parent.
4) sterile line breeding.Using bj1841 as sterile source, using DH1633-1 as recurrent parent, after 6 generation of continuous backcross
(BC6), the ogura cytoplasmic male sterile line for obtaining inheritance stability, is denoted as DH1633-1A.
5) purple male parent breeding.2014 with the purple cauliflower cenospecies C14302 that obtains in the market for F1In generation, passes through
Selfing, separation F in 20152For more than 300 strain of group, it is screened out from it more than 40 strain of single plant of bouquet purple depth, bouquet rounding, is continued
Individual plant selfing is reserved seed for planting, the F fertile to more than 40 strains in 20163Field test is carried out for strain, filters out that bouquet is dark purple not to be separated
2 strains carry out microspores culture, be denoted as PCK-1, PCK-3, therefrom screening obtain purple pagoda-shaped dihaploid DH homozygosis plant
Strain column, are denoted as DH6915, filter out bouquet darkviolet DH pure lines, are denoted as DH6915-1, show as dark green leaf color, and lobus cardiacus is purple
Color, bouquet darkviolet, tight circle, bennet and bud are purple, and floral organ development is normal.
6) purple pagoda cauliflower is breeding hybridized.It is mother with pagoda-shaped ogura cytoplasmic male sterile line DH1633-1A
This, using purple cauliflower DH6915 as male parent, prepares a large amount of hybrid combinations, by Field Screening, determines with DH6915-1 to be father
This cross combination is excellent in.It is prosperous that its cenospecies shows as plant strain growth gesture, bouquet purple, pagoda point, and Comprehensive Traits are excellent.
7) production of cenospecies.Using putting bee method breeding hybrid kind in greenhouse.
Embodiment 2
Because pagoda cauliflower needs repeatedly cut ball and overwintering cultivation, winter habit is stronger, needs to breed parent in greenhouse, in order to avoid
Wound after cutting ball is influenced introduced disease by rainwater.Recurrent parent can be awarded because of self-compatible in independent canopy by putting bee auxiliary
Powder obtains seed；Maternal sterile line plants sterile line and recurrent parent in independent canopy with the ratio of 1:1, needs to award by putting bee
Powder carries out backcrossing and obtains seed；Male parent is self-compatible DH system, needs to put bee in greenhouse and obtains seed.
Embodiment 3
If first-filial generation kind needs production of hybrid seeds amount bigger, can collect seed finding the outdoor cold bed isolation of appropriate area progress,
As a kind of special vegetable, the isolation production of hybrid seeds can be carried out in greenhouse under normal circumstances.
1) it selects planting greenhouse: according to production region, selecting common plastics greenhouse, glasshouse or heliogreenhouse, preceding work
It is preferably no and planted crop in cruciferae or plastic shed soil carried out sterilizing.Base manure and borax are used sufficient before transplanting.
2) sowing and field planting ratio: parent's culturing and transplanting seedlings, according to female parent: the ratio field planting of male parent 2:1 or 3:2.Because of female parent
Breeding time 15 days slower than male parent or so, need sow within 15-20 days ahead of time.By taking In Hangzhou Region of Zhe Jiang Province as an example, maternal sowing time is in August
The sowing time in ten days, male parent is late August, and seedling age 30 days or so, next year late March was bloomed.
3) florescence adjusts: in addition to sowing time is staggered, because of annual Changes in weather, normally meeting at the florescence, is needed to father for parents
This is regulated and controled by cutting the measures such as ball, rich water control.Maternal Chinese artichoke cuts ball for the first time and stays a level-one branch bouquet, and second
The secondary ball that cuts only stays a second branch again, after second branch bolting, only stays 20-30 three-level branch；Male parent will be according to female parent
After the bolting situation of bouquet determines that cutting ball for the first time stays a level-one branch bouquet, second of second branch cuts ball method, to mention
Early male parent is allowed to bloom, it is desirable that cut edge edge second branch stays intermediate 20-30 second branch.To allow male parent to bloom ahead of time, can subtract
Shaoshi fertilizer and increase irrigation times method are regulated and controled, and delay can take opposite approach.
4) rich water and pest management: the seedling management of pagoda cauliflower is the same as general cauliflower.The production of hybrid seeds rich water suitably to control
System reduces the generation of the diseases such as soft rot, sclerotiniose convenient for control bouquet size.Rush base fertilizer is applied, appropriate top dressing increases potash fertilizer,
Especially bouquet bolting Post flowering imposes the compound fertilizer based on potassium, and the knot angle phase regards plant strain growth situation application of phosphoric acid potassium dihydrogen
Foliar fertilizer.The prevention and treatment for paying special attention to bouquet and knot angle phase sclerotiniose and diamondback moth will stop for 15 days before putting bee because of honeybee pollination
Medication, blooming terminates final finishing plant, prevents and treats in time.
5) it harvests: being pulled out in time after the male parent florescence in time, the seed harvested from maternal sterile line is to hybridize one
For seed.Silique yellow maturity cracking before in time whole strain or in batches branch item harvest, stack after-ripening in one week or so, after by the gross maturation after
Threshing impurity elimination, is dried, label, packaging.
Purple pagoda-shaped cauliflower cenospecies, Mid-late ripening harvest for 90 days or so after field planting；Plant growing way is strong, and plant type half is straight
It is vertical, blade circle；Bouquet purple, pagoda-shaped, pinnacle of a pagoda, bennet purple；Bouquet list ball weight 0.8kg or so；The cenospecies comprehensive resistance
Excellent, bouquet partly covers leaf, and bouquet is bright in colour, is rich in anthocyanin, and have ornamental value.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understand without departing from the principles and spirit of the present invention can to these examples carry out it is a variety of variation, modification, replacement and
Modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of selection of purple pagoda cauliflower cenospecies, which comprises the following steps:
It 1) is female parent with the pagoda cauliflower Hybrid of the ogura cytoplasmic male sterility of introduction, with excellent pagoda flower coconut palm
Dish Doubled haploid line is male parent, is hybridized；
2) transformation generation plant filters out the sterile single plant bloomed with Normal nectary, using excellent Doubled haploid line as circulation parent
This, carries out obtaining the ogura cytoplasmic male sterile line of inheritance stability mostly for backcross transformation；
3) bouquet circle and purple cauliflower germplasm are introduced, obtains F after being selfed for 1 generation2For group, screening bouquet purple is deep, bouquet is round
Whole single plant carries out microspores culture or selfing separation, therefrom obtains purple dihaploid homozygous plants (DH system) and purple height generation
Inbred pure line filters out the excellent dihaploid pure lines of bouquet purple depth；
4) cytoplasmic male sterile line of the inheritance stability obtained with transformation is female parent, the purple DH system obtained with microspores culture
For male parent, preparing hybrid combination；
5) it is female parent that screening, which is obtained with the dihaploid sterile line, using dihaploid purple cauliflower as the cenospecies of male parent, is educated
At excellent purple pagoda-shaped cauliflower cenospecies.
2. a kind of selection of purple pagoda cauliflower cenospecies according to claim 1, which is characterized in that step
(1) ogura sterile source pagoda cauliflower material flower bud phase cabage-type rape hybridizes in, obtains a cytoplasmatic male transformation generation.
3. a kind of selection of purple pagoda cauliflower cenospecies according to claim 1, which is characterized in that step
(2) recurrent parent self-compatible in after 6 generation of continuous backcross, obtains the pagoda-shaped cauliflower cytoplasmic male sterility of inheritance stability
System makees maternal.
4. a kind of selection of purple pagoda cauliflower cenospecies according to claim 1, which is characterized in that step
(3) described in purple cauliflower segregating generation F3On behalf of microspores culture donor, the excellent DH of bouquet purple depth is filtered out
System makees male parent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115735760A (en) * | 2022-11-28 | 2023-03-07 | 宁波微萌种业有限公司 | Purple cauliflower breeding method |
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Application publication date: 20191008 |