CN110338053B

CN110338053B - Breeding method of orange pagoda cauliflower hybrid

Info

Publication number: CN110338053B
Application number: CN201910741463.6A
Authority: CN
Inventors: 顾宏辉; 赵振卿; 王建升; 虞慧芳; 盛小光; 沈钰森
Original assignee: Zhejiang Academy of Agricultural Sciences
Current assignee: Zhejiang Academy of Agricultural Sciences
Priority date: 2019-08-12
Filing date: 2019-08-12
Publication date: 2021-06-04
Anticipated expiration: 2039-08-12
Also published as: CN110338053A

Abstract

The invention discloses a breeding method of orange pagoda cauliflower hybrid, which uses a pagoda orange DH line or an inbred pure line which is obtained by microspore culture or is homozygous by traditional breeding inbred as a male parent, uses a common pagoda cauliflower ogura cytoplasmic male sterile line obtained by multi-generation backcross transformation as a female parent, and obtains an orange pagoda cauliflower hybrid first-generation new variety by a hybridization way. The orange pagoda cauliflower obtained by the method is prepared from an ogara cytoplasmic male sterile line, and the recurrent parent is self-compatible, so that bee pollination can be performed to propagate hybrid seeds and the sterile line, and the seed production cost is greatly reduced.

Description

Breeding method of orange pagoda cauliflower hybrid

Technical Field

The invention belongs to the technical field of plant breeding, and particularly relates to a breeding method of orange pagoda cauliflower hybrid.

Background

Pagoda cauliflower (A. chinensis)Brassica oleracea L. var. botrytis cv. romanesco) Also known as Roman cauliflower, first described in Italy of the 16 th century, is a type of broccoli variety of the Brassica oleracea species of the Brassicaceae. The vegetable is introduced into China from the end of the twentieth century and is planted in a small amount in China as a special vegetable. The Palettuces are rich in vitamin C, K, crude fiber and carotenoid, and flower balls of the Palettuces are arranged in a Pennella cut-number array in a fractal manner, so that the Palettuces are both nutritional and ornamental.

The ordinary pagoda cauliflower seeds come from abroad, and the orange pagoda cauliflower variety does not appear or research work on breeding at home and abroad. The germplasm resources of the common pagoda cauliflower are few, most of the pagoda cauliflower are sterile hybrid seeds, and the available resources are rare. Therefore, if germplasm innovation and breeding utilization can be carried out on rarely available resources, the types of the pagoda cauliflowers are enriched, and the new types which are delicious and good are created, the requirements of the market on variety type diversification can be met.

Based on the original germplasm resources of the pagoda cauliflower, the applicant combines a microspore culture technology and a traditional cross breeding technology, and utilizes a cytoplasmic male sterile line approach to breed the orange pagoda cauliflower hybrid which has independent intellectual property rights, is delicious and has ornamental value.

Disclosure of Invention

The invention aims to breed an orange pagoda cauliflower hybrid variety with independent intellectual property rights. The method comprises the steps of utilizing a microspore culture technology to quickly obtain homozygous breeding parents, breeding a hybrid female parent by combining a cytoplasmic male sterility approach, and breeding a first-filial generation new variety of orange pagoda cauliflower with high carotene content and ornamental value through hybrid seed production.

The invention is realized by the following technical scheme:

a breeding method of orange pagoda cauliflower hybrid seeds comprises the following steps:

1) and hybridizing the cytoplasmic male sterile pagoda cauliflower hybrid variety serving as a female parent and the excellent doubled haploid line serving as a male parent to obtain the first generation of the ogura cytoplasmic male sterile transfer.

2) And (4) selecting sterile single plants which are flowering and have normal development of honey glands from the first transfer generation, and carrying out multi-generation backcross by using an excellent double haploid line with self-compatibility as a recurrent parent to obtain the genetic stable ogura cytoplasmic male sterile line.

3) Hybridizing fertile pagoda cauliflower with orange cauliflower to obtain orange pagoda cauliflower hybrid F₁And performing microspore culture or selfing separation on the hybrid to obtain an orange pagoda-type double haploid homozygous plant and an orange pagoda-type high-generation selfing pure line, and screening out a flower ball orange and pagoda-type excellent double haploid pure line.

4) And (3) preparing a hybrid combination by taking the genetically stable ogura cytoplasmic male sterile line obtained by transformation as a female parent and taking an orange pagoda-type double haploid pure line obtained by microspore culture as a male parent. Since the globus orange gene is dominant, the progeny hybrids obtained appear globus orange.

5) Screening to obtain the hybrid with double haploid ogura cytoplasmic male sterile line as female parent and double haploid orange pagoda cauliflower as male parent, and breeding to obtain the excellent orange pagoda cauliflower hybrid.

Further, in the step (1), a superior self-compatible pagoda cauliflower double haploid line is taken as a recurrent parent, and pollination hybridization is carried out in the bud period to obtain a first generation of the ogara cytoplasmic male sterile transformation.

Further, after 6 generations of continuous backcross in the step (2), a genetic stable ogura cytoplasmic male pagoda type cauliflower sterile line is obtained as a female parent.

Further, in the step (3), the first filial generation of the fertile pagoda cauliflower and the orange cauliflower is used as a microspore culture donor, and a pagoda-shaped excellent double haploid DH pure line with an orange flower ball is screened out to be used as a male parent.

The invention has the beneficial effects that:

1) the obtained orange pagoda cauliflower hybrid has an orange ball, a pagoda tip shape and excellent field comprehensive character performance.

2) Compared with the common pagoda-shaped cauliflower, the hybrid ball has orange color, high carotene content and no color change when being fried and boiled at high temperature.

3) Because the father and the mother are both from DH line, the purity is high, the obtained orange pagoda cauliflower hybrid is neat and consistent, and the market time of the ball is concentrated.

4) The obtained orange pagoda cauliflower is prepared by a cytoplasmic male sterile line, and the recurrent parent is self-compatible, so that honeybee pollination can be used for breeding hybrid and the sterile line, and the seed production cost is greatly reduced.

Drawings

FIG. 1 is a flow chart of the breeding of hybrid of the present invention.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

As shown in fig. 1, the present embodiment provides a breeding method of an orange pagoda cauliflower hybrid, which specifically includes the following steps:

1) and (4) germplasm resource screening. From 2010, 7 pagoda-type cauliflower and 2 orange cauliflower germplasm resources were collected. Through agronomic character observation and identification, 1 part of male sterile pagoda type cauliflower, 3 parts of fertile pagoda type cauliflower and 2 parts of available orange cauliflower germplasm which can be bred and utilized are screened out.

2) And (4) selecting a male sterile source. The male sterile pagoda-type cauliflower hybrid is marked as bj1841, shows normal petals and honey glands and is used as a male sterile source breeding material.

3) And (5) recurrent parent breeding. Fertile pinnate pagoda type cauliflower hybrid seeds are used as donors and are marked as RCK01, double-haploid series 13 strains are obtained through microspore culture and are marked as DH1633, wherein DH1633-1 shows that pinnate leaves, self-compatibility, light green flower balls and pagoda tips, flower organs at the flowering period are normally developed and are used as recurrent parents of sterile female parents.

4) And (5) breeding the sterile line. After continuous backcross for 6 generations (BC) with bj1841 as sterile source and DH1633-1 as recurrent parent₆) And obtaining a genetic stable cytoplasmic male sterile line which is marked as DH 1633-1A.

5) And (5) breeding the orange pagoda male parent. Hybridizing fertile leaf round-leaf type pagoda cauliflower (marked as RCK 03) with orange fertile cauliflower material (marked as C15502) to obtain orange pagoda cauliflower hybrid F₁And (4) generation. To obtain F₁And (3) carrying out microspore culture on the hybrid seeds serving as microspore donor materials, screening to obtain a series of homozygous orange pagoda double haploid DH (marked as DH 5617), and screening a pure cone orange pagoda DH (marked as DH 5617-13), wherein the pure cone orange floral DH is shown as a cone orange, a cone tip and more branches, and the floral organs are normally developed.

6) Orange pagoda cauliflower hybrid seed selection. The hybrid combination prepared by using pagoda type cytoplasmic male sterile line as female parent (DH 1633-1A) and orange pagoda type as male parent (DH 5617-13) shows that the plant grows vigorously, the flower ball is orange, the pagoda tip is sharp, and the comprehensive character is excellent.

7) And (5) producing hybrid seeds. And breeding the hybrid seeds by adopting a method of releasing bees in a greenhouse.

Example 2 parental breeding

Because the pagoda cauliflower needs to be cut into balls for multiple times and cultivated in winter, the parents need to be bred in a greenhouse, and the wounds after the ball is cut are prevented from being infected with diseases due to rainwater. The recurrent parent is self-compatible, and seeds can be obtained by bee releasing and supplementary pollination in a single shed; planting the sterile line and recurrent parent in a single shed of the female parent sterile line according to the proportion of 1:1, and carrying out backcross by putting bees for pollination to obtain seeds; the male parent is self-incompatible DH line, and the seeds are obtained by artificial bud stripping and pollination in the greenhouse during the flowering period.

EXAMPLE 3 hybrid seed production

If the first-filial generation seeds need to be produced in a larger quantity, open-air cold-ridge isolated seed production can be carried out in a suitable area, and isolated seed production can be carried out in a greenhouse under general conditions.

1) Selecting a planting greenhouse: according to the production region, selecting common plastic greenhouse, glass greenhouse or sunlight greenhouse, preferably not planting cruciferous crops in the former crop, or sterilizing greenhouse soil. And applying enough base fertilizer and borax before transplanting.

2) Sowing and planting ratio: parent seedling and transplanting, according to the female parent: and (3) permanent planting of the male parent in a ratio of 2:1 or 3: 2. Because the growth periods of the female parent and the male parent are close, the seeds can be sowed and transplanted at the same time. Taking the Hangzhou area as an example, the seeding period of the female parent and the male parent is the middle ten days of 8 months, the seedling age is about 30 days, and the female parent and the male parent bloom in the next 3 months.

3) Fertilizer water and pest management: the seedling management of the pagoda cauliflower is the same as that of the common cauliflower. The fertilizer water for the land preparation needs to be properly controlled, so that the size of the flower ball is convenient to control, and the diseases such as soft rot, sclerotinia and the like are reduced. Applying base fertilizer, top dressing, potassium fertilizer, especially applying compound fertilizer with potassium as main component after bolting and blooming of flower ball, and applying potassium dihydrogen phosphate foliage fertilizer according to plant growth condition in the angulus formation stage. Especially paying attention to the control of sclerotinia rot and diamond back moth in the flowering and fruiting periods, because bees pollinate, the application of the pesticide is stopped 15 days before the bees are put, and plants are arranged after the blossoming is finished for timely control.

4) Timely harvesting: timely removing the male parent after the flowering phase of the male parent is finished, and obtaining the seeds harvested from the female parent sterile line as the first filial generation seeds. Harvesting whole plants or branch strips in batches in time before yellow ripe cracking, stacking for about one week, then ripening, threshing after the whole batch is ripe, removing impurities, drying in the sun, labeling and packaging.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A breeding method of orange pagoda cauliflower hybrid is characterized by comprising the following steps:

1) performing pollination hybridization in a bud period by taking the hybrid variety of the Opura cytoplasmic male sterile Ziziphus jujuba as a female parent and taking the excellent self-compatible double haploid line of the Ziziphus juba as a male parent to obtain a first generation of the OGura cytoplasmic male sterile transfer;

2) screening sterile single plants which are transferred to one generation and are normally flowering and honeygland development, and carrying out multi-generation backcross by taking the excellent self-affinity pagoda cauliflower double haploid line as a recurrent parent to obtain an eura cytoplasmic male pagoda cauliflower sterile line with stable heredity;

3) hybridizing fertile pagoda cauliflower with orange cauliflower to obtain orange pagoda cauliflower hybrid F₁Carrying out microspore culture or selfing separation on the hybrid to obtain an orange pagoda-type double haploid homozygous plant and an orange pagoda-type high-generation selfing pure line, and screening out a flower ball orange and pagoda-type excellent double haploid pure line;

4) preparing a hybrid combination by taking the genetically stable ogura cytoplasmic male sterile line obtained by transformation as a female parent and taking an orange pagoda-type double haploid pure line obtained by microspore culture as a male parent;

5) and (4) screening the pagoda cauliflower with the flower ball being orange and the tip of the pagoda, and breeding the excellent orange pagoda cauliflower hybrid.

2. According to the claimsThe breeding method of the orange pagoda cauliflower hybrid seed in the step (1) is characterized in that the orange pagoda cauliflower hybrid seed F is obtained in the step (3)₁The breeding is replaced by a microspore culture donor, and a pagoda-shaped excellent double haploid DH pure line with a flower ball orange color is screened out to be used as a male parent.

3. The method for breeding the orange pagoda cauliflower hybrid as claimed in claim 1, wherein the pagoda type ogara cytoplasmic male sterile line with self-compatibility as recurrent parent is used as female parent in step (4).