CN106818226B - Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees - Google Patents
Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees Download PDFInfo
- Publication number
- CN106818226B CN106818226B CN201710048089.2A CN201710048089A CN106818226B CN 106818226 B CN106818226 B CN 106818226B CN 201710048089 A CN201710048089 A CN 201710048089A CN 106818226 B CN106818226 B CN 106818226B
- Authority
- CN
- China
- Prior art keywords
- persimmon
- plant
- seed production
- parent
- male
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention provides a persimmon tree multi-variety top-grafting head-changing hybrid seed production method, which comprises the following steps: taking the place where no male persimmon tree germplasm resources exist within 10-15 km of a square circle as a hybrid planting field; selecting a persimmon tree without plant diseases and insect pests on the hybrid seed production land as a mother plant; grafting the male parent and different varieties which only bloom female flowers and do not have male flowers as female parents onto the stock plant, and growing for 2-3 years to produce seeds; the variety of the male parent is kaki, autumn or late autumn; the male parent is grafted on the top end of the mother plant, and the female parent is grafted around the mother plant. By adopting the hybrid seed production method, 7519-8162 hybrid seeds can be obtained on 1 parent plant which is 30-40 years old every year, which is far higher than that of the traditional hybrid seed production method (367 seeds), and the seed production efficiency is improved.
Description
Technical Field
The invention belongs to the technical field of agriculture and forestry breeding methods, and particularly relates to a persimmon tree multi-variety high-grafting head-changing hybrid seed production method.
Background
Persimmon (Diospyros kaki Thunb.) belongs to the genus Diospyros (Ebenaceae) of the family Ebenaceae, and most varieties only bear female flowers, very few varieties are hermaphroditic plants, and the whole plant bears only male flowers very rarely. 560 parts of persimmon variety resources collected in a persimmon germplasm resource garden in China only have 22 parts of growing male flowers. Therefore, it is very difficult to select the male parent with good characteristics in persimmon, so the process of transferring good genes to offspring has great blindness, and the capacity of seed formation after crossing is greatly different among different varieties, so that conventional cross breeding is difficult. However, conventional hybrid breeding is still the most effective means for persimmon tree breeding at present, for example, the sweet persimmon varieties 'Yangfeng', 'Xianlang', 'early autumn', 'sweet autumn', 'late autumn' and the like which are mainly planted at present are all obtained by artificial hybrid breeding, and the persimmon varieties bred by the hybrid breeding generally show unprecedented excellent quality, are favored by the market and account for more than 80% of the current cultivation area. Therefore, the cultivation of new persimmon varieties through cross breeding at the present stage still has great practical significance.
However, the traditional artificial hybridization breeding needs complicated procedures such as pollen collection, hybridization, bagging and the like, consumes a large amount of manpower and financial resources, and causes the female flower stigma to be damaged through the artificial hybridization, the fruit setting rate is between 9.0 and 24.0 percent, the average fruit setting rate is 15 percent, so the fruit setting rate is reduced, and the seed production rate is low.
Disclosure of Invention
In view of the above, the present invention provides a method for producing hybrid seeds of multiple varieties of persimmon trees by top grafting and head changing, which can improve the seed production rate of persimmon trees, in view of the shortcomings of the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
a persimmon tree multi-variety top-grafting head-changing hybrid seed production method comprises the following steps:
1) taking the place where no male persimmon tree germplasm resources exist within 10-15 km of a square circle as a hybrid planting field;
2) selecting a persimmon tree without plant diseases and insect pests as a mother plant on the hybrid seed production land in the step 1);
3) taking different persimmon tree varieties which only bloom female flowers and do not bloom male flowers as female parents;
4) grafting the male parent and the female parent in the step 3) onto the stock plant in the step 2), and growing for 2-3 years to produce seed;
the female parent is one or more of Qingzhou small calyx, Spearmint persimmon, Kigelia japonica, Taian small batter, locomotive persimmon, Fuping jian persimmon, Maocheng persimmon, Boai Baoyue yellow, Mopan persimmon, Pingguan, Yangfeng, early autumn, Xinjin 20, Xianlang, black persimmon, oil creeper head, Japanese compass, lotus persimmon, fire crystal, September, flagpole top and cinnabar red;
the male parent is kaki, autumn or late autumn;
the male parent is grafted on the top end of the mother plant, and the female parent is grafted around the mother plant, so that at least one head is ensured to survive the grafting.
Preferably, the stock plant is a 30-40 year old persimmon tree.
The invention provides a persimmon tree multi-variety top-grafting head-changing hybrid seed production method, which comprises the following steps: 1) taking the place where no male persimmon tree germplasm resources exist within 10-15 km of a square circle as a hybrid planting field; 2) selecting a persimmon tree without plant diseases and insect pests as a mother plant on the hybrid seed production land in the step 1); 3) taking different persimmon tree varieties which only bloom female flowers but do not bloom male flowers as female parents; 4) grafting the male parent and the female parent in the step 3) onto the stock plant in the step 2), and growing for 2-3 years to produce seed; the female parent is one or more of Qingzhou small calyx, Spearmint persimmon, Kigelia japonica, Taian small batter, locomotive persimmon, Fuping jian persimmon, Maocheng persimmon, Boai Baoyue yellow, Mopan persimmon, Pingguan, Yangfeng, early autumn, Xinjin 20, Xianlang, black persimmon, oil creeper head, Japanese compass, lotus persimmon, fire crystal, September, flagpole top and cinnabar red; the male parent is kaki, autumn or late autumn; the male parent is grafted on the top end of the mother plant, and the female parent is grafted around the mother plant, so that at least one head is ensured to survive the grafting. In the invention, the kaki, the autumn or the autumn serves as a male parent, and can pollinate other excellent varieties after multi-variety grafting, and the damage of artificial hybridization to female flower stigma is reduced, the forming capability of seeds is promoted, and the seed production rate is improved. By adopting the hybrid seed production method, 7519-8162 hybrid seeds can be obtained on 1 mother tree which is 30-40 years old every year, which is far higher than that of the conventional hybrid seed production method (367 seeds), and the seed production efficiency is improved.
Detailed Description
The invention provides a persimmon tree multi-variety top-grafting head-changing hybrid seed production method, which comprises the following steps:
1) taking the place where no male persimmon tree germplasm resources exist within 10-15 km of a square circle as a hybrid planting field;
2) selecting a persimmon tree without plant diseases and insect pests as a mother plant on the hybrid seed production land in the step 1);
3) taking different persimmon tree varieties which only bloom female flowers and do not bloom male flowers as female parents;
4) grafting the male parent and the female parent in the step 3) onto the stock plant in the step 2), and growing for 2-3 years to produce seed;
the female parent is one or more of Qingzhou small calyx, Spearmint persimmon, Kigelia japonica, Taian small batter, locomotive persimmon, Fuping jian persimmon, Maocheng persimmon, Boai Baoyue yellow, Mopan persimmon, Pingguan, Yangfeng, early autumn, Xinjin 20, Xianlang, black persimmon, oil creeper head, Japanese compass, lotus persimmon, fire crystal, September, flagpole top and cinnabar red;
the male parent is kaki, autumn or late autumn;
the male parent is grafted on the top end of the mother plant, and the female parent is grafted around the mother plant, so that at least one head is ensured to survive the grafting.
In the invention, the stock plant is a persimmon tree without plant diseases and insect pests for 30-40 years; the male parent is kaki, autumn or late autumn, and is grafted on the top of the mother plant to ensure that at least one head survives; the female parent is a good main cultivar of persimmon trees in the main producing area of China, only female flowers are bloomed, male flowers are not bloomed, and specifically, the female parent is one or more of Qingzhou small calyx, lotus mirror persimmon, jinqi persimmon, Taian small batter, locomotive persimmon, Fuping jian persimmon, Maocheng Yueyu persimmon, Boeiyue yellow, Mill persimmon, Pingguowu, Yangfeng, early autumn, Xingjin 20, Xianlang, black persimmon, oil basket head, Japanese compass, lotus flower persimmon, fire crystal, September green, flagpole top and cinnabar red, and the female parent is grafted around the parent plant, and each variety is ensured to survive for at least 1 head. In the present invention, the source of the female parent is not particularly limited, and a variety conventionally selected by those skilled in the art may be used.
In the present invention, the male parent is kaki, fall or fall. In the present invention, the source of the male parent is not particularly limited, and may be one conventionally selected by those skilled in the art.
In the embodiment of the present invention, the scions of the female parent and the male parent are preferably collected by self, and the collecting steps are specifically: collecting annual development branches or fruiting mother branches with pure varieties, no malformed fruits and plant diseases and insect pests and full development in the persimmon tree dormancy stage, and preserving moisture and storing in sand after the persimmon tree is picked, wherein the persimmon tree dormancy stage can be used at any time.
After the scions of the female parent and the male parent are collected, if the scions are not used immediately, preferably, the scions of the female parent and the male parent are subjected to sand storage; in an embodiment of the present invention, the step of preserving is preferably embodied as: the cut scions are respectively bound into bundles in a classified mode, marked and buried in cool and slightly wet sandy soil to prevent water evaporation so as to keep the vitality of the scions and delay the germination time of branches. Before the scions are buried in the sandy soil, the scions are preferably sealed with paraffin so as to better prevent water evaporation. Before use, the scions buried in sandy soil are taken out and then wrapped by wet cloth or wet newspaper to prevent water loss.
The scions of the female parent and the male parent are grafted when the female plant germinates, and the specific grafting method comprises the following steps: the scions of the female parent and the male parent are grafted on the skeletal branch or the central leading branch of the stock plant, and before grafting of the scions, the stock plant is preferably trimmed and shaped so as to leave a position for grafting. The grafting mode preferably adopts tongue grafting or double-tongue grafting.
In the invention, the male parent is grafted on the top of the mother plant; the female parent is grafted around the mother plant.
After the scion grafting of the female parent and the male parent is completed, the female parent and the male parent are guaranteed to preferentially and independently survive for at least 1 head, and the branches which are not grafted are recutted, so that the grafted female parent and the grafted male parent grow synchronously. After the grafting of the female parent and the male parent is finished, binding and fixing are preferably carried out on the female parent, the male parent and the female plant, the binding and fixing method is not particularly limited, and the conventional binding and fixing in the field can be adopted.
The preferable selection of the growth management of the persimmon trees after grafting comprises timely sprout removal of the branches of the mother plants which are not grafted, and timely watering is needed when the soil is dry. When the new bud grows to 35-45 cm, the binding is removed, and the scion bud which does not survive is timely replanted. In order to prevent the branches with high joints from being blown and damaged, the branches are bound by extending the growth direction of the new tips through small bamboo poles, and the core picking and the big leaf picking can be combined to promote the lignification of the new tips. The disease and insect pest of the persimmon trees are preferably controlled mainly and combined. In early spring, sap begins to flow, and before germination, 5 Baume lime sulphur is sprayed on the whole stock plant to kill various germs and overwintering ova. When 3-5 leaves grow on a new tip, 0.1% of AVG persimmon tree fruit retention and anti-falling agent is sprayed on the whole tree, the persimmon tree fruit retention and anti-falling agent is sprayed once after 20-25 days, the spraying is carried out until the inside and the outside of a tree chamber are uniformly sprayed, the leaves are sprayed with water to be dripped optimally, and the purpose is to increase the nutrition of the leaves, promote the growth of the new tip and accelerate healing.
The following will explain the persimmon tree multi-variety top-grafting head-changing hybrid seed production method provided by the present invention in detail with reference to the examples, but they should not be construed as limiting the scope of the present invention.
Comparative example
Research on fruit setting rate of different hybridization combinations of traditional persimmon trees
Test materials: the test was carried out in 2014 in a test base of the Wangeshan persimmon tree in Taishan district, Taian City, Shandong, and 10 female parent varieties were tested: semen Clerodendranthi Cyrtophylli, herba Menthae Pileae Scriptae, JINZHU, TAIAN XIAOHU, TITU, FUPINGJIAN, GONGCHEYUEZI, BOAIBAOYUHUANG, JIAOLIU, and PINGGUOWU.
And (3) combination and matching: according to the principle of consistent flowering period, 10 hybrid combinations of the small calyx seu fructus kaki, the large specularia persimmons, the large calyx kaki, the small flour paste with high safety, the large head persimmons, the large tippy persimmons, the large clitora persimmons and the large clitora persimmons are selected.
Pollen collection: collecting 100 flowers containing buds to be placed in the initial flowering period of a test variety of persimmons to bring the flowers back to a laboratory, poking the petals with forceps, gently taking down the anthers, naturally drying the taken-down anthers at room temperature to loose powder, and keeping the anthers at room temperature for 24 hours to completely loose the powder. Collecting pollen after pollen scattering, labeling in a clean small bottle, and storing in a refrigerator at 4 deg.C for use.
And (3) field cross pollination: selecting 3 flowers containing buds on each bearing branch of 10 female parent varieties to be tested in the full-bloom stage, dipping a small amount of persimmons with a rubber head to perform spot pollination on the stigma, then bagging, thinning the rest flowers, and performing combined treatment on more than 100 flowers by each cross pollination. The number of the 10 hybridized flowers is 1301.
Fruit setting rate statistics: and (5) picking bags 5-7 days after the final flowering phase of the tested variety, and respectively counting the fruit setting rate of each hybridization combination before fruit picking. The formula of the investigation fruit setting rate is as follows: fruit set (%). fruit number/pollinated flower number × 100. 195 total fruit bearing fruits are obtained by 10 hybridization combinations, and the average fruit bearing rate is 15.0 percent.
Counting the number of seeds: after the fruits are ripe, picking the fruits according to different hybridization combinations, marking and classifying and harvesting. Removing pulp, reserving seeds to obtain hybrid seeds, and counting the number of seeds of each hybridization combination respectively, wherein the number of hybrid seeds harvested by 10 hybridization combinations is 367, and the average number of hybrid seeds harvested by each hybridization combination is 36.7. The fruit set rate and seed number results are shown in table 1.
TABLE 1 fruit set rate and seed number for different hybridization combinations
Example 1
Multi-variety top-grafting head-changing hybrid seed production for persimmon trees
Selecting a good dry and fresh dual-purpose variety 'locomotive persimmon' which has robust growth potential and no disease and insect damage as a parent plant in Huangzhuang Zhenchangshengcun in a steel urban area of Laiwu city in Shandong province in 2012 and 4 months. And simultaneously and respectively grafting 10 excellent varieties (as female parents) of lotus specularia persimmon, jinqi persimmon, Taian small batter, Qingzhou small calyx, Fuping jian persimmon, Maocheng persimmon, Boai Baoyue yellow, Maochun persimmon, Pinggui and Bopo persimmon on the headstock persimmon in a multi-head high mode, and naturally pollinating after grafting. During grafting, the epipodophyllum kaki with a blooming flower is grafted at the top end of a mother plant (as a male parent), the other 9 varieties are respectively grafted at the periphery of the mother plant, and each variety is ensured to survive for at least 1 head; and after grafting is finished, all branches which are not grafted are re-cut, so that the branches which are not grafted and the grafted branches grow synchronously. During the period, the crown can be recovered in 2013 by conventional management, and seeds of 10 varieties of fruits are collected in 2014, 2015 and 2016 continuously and respectively. The number of seeds harvested for 10 varieties is shown in Table 2.
TABLE 2 number of seeds of many varieties of persimmon tree harvested three consecutive years after top grafting and crop changing
Example 2
In 2011, in Shandong province, Lin 26384in Wujing Garden river village in county, five villages, the monarch emisson (a common stock of a northern main persimmon tree variety) which has robust growth potential and no diseases and insect pests is selected as a stock plant in 37 years. And simultaneously and respectively inoculating 12 varieties of Yangfeng, early autumn, Xingjin 20, Xilang, Heishu, Jingyan, Fuping, Maocheng, Pangshui, Pinggu, Pinggui and Taiqiu on the parent plant of the kaffir. The 12 varieties only have the same plants of the male and female in autumn, namely the female flowers and the male flowers are bloomed; the other 11 varieties only bloom female flowers, but not male flowers. And (5) natural pollination is carried out after grafting. During grafting, the late autumn of the blooming flower is grafted at the top end of a stock plant, the other 11 varieties are respectively grafted at the periphery of the stock plant, and each variety is guaranteed to survive for at least 1 head; and after grafting is finished, all branches which are not grafted are re-cut, so that the branches which are not grafted and the grafted branches grow synchronously. During the period, the crown can be recovered in 2012 by conventional management, and seeds of 11 varieties of fruits are collected in 2013, 2014 and 2015 for three consecutive years. The number of seeds harvested for 11 varieties is shown in Table 3.
TABLE 3 number of seeds of many varieties of persimmon tree harvested three consecutive years after top grafting and crop changing
Example 3
'Yishui persimmon' which is robust in growth and free of diseases and insect pests in 35 years is selected as a mother plant in a Diospyros kaki orchard village in waterlogged sloping towns in Junan county of Shandong in 2012 4 months. And 13 varieties of early autumn, Xingjin 20, Xianlang, Yangfeng, black persimmon, oil basket head, Japanese compass, lotus persimmon, flint, September, flagpole top, cinnabar and Ganqiu are simultaneously and respectively connected on the stock plant in a multi-head manner. Only the 13 varieties have the same plants of the male and the female in the autumn, namely, the female flowers and the male flowers are bloomed; the rest 12 varieties only bloom female flowers but not male flowers. And (5) natural pollination is carried out after grafting. During grafting, the autumn of the blossoming male flowers is grafted at the top end of a stock plant, the rest 12 varieties are respectively grafted at the periphery of the stock plant, and each variety is guaranteed to survive for at least 1 head; and after grafting is finished, all branches which are not grafted are re-cut, so that the branches which are not grafted and the grafted branches grow synchronously. During the period, the crown can be recovered in 2013 by conventional management, and seeds of 12 varieties of fruits are collected in 2014, 2015 and 2016 continuously and respectively. The number of seeds harvested for the 12 varieties is shown in Table 4.
TABLE 4 number of seeds of many varieties of persimmon tree harvested three consecutive years after top grafting and crop changing
From the comparative example and the experimental results of examples 1 to 3, it can be obtained that the traditional artificial crossbreeding requires tedious procedures of pollen collection, hybridization, bagging and the like, a large amount of manpower and financial resources are consumed, 1 hybridization combination is required for pollen collection and preparation, 1 worker is required for pollen collection and preparation, 3 workers are required for manual ordering and bagging, 6 workers are required for 2 days, 2 workers are required for 1 day after fruit setting and bagging is required for 2 workers, the calculation is performed according to 100 yuan for each worker, thus the total amount of 900 yuan is only by one worker cost, 9000 yuan is required for 10 hybridization combinations, the number of harvested seeds is only 367, and 2.45 yuan is required for obtaining 1 seed on average.
On the basis of variety resources without the male flowers around the persimmon trees, multiple varieties of high-grafting head-changing natural hybrid pollination are adopted, one-time grafting can be performed, seeds are harvested for multiple years, 1 grafting worker grafts 10-12 excellent persimmon tree varieties (persimmon varieties containing 1 male flower) on one persimmon tree stock plant, the grafting work can be completed within 2 hours, 1 worker is enough in the period of conventional management, hybrid seeds can be obtained on 1 30-40-year-old mother tree as high as 7519-8162 seeds every year, the seed production cost is far lower than that of conventional hybrid seed production, and the seed production efficiency is improved.
According to the comparative example and the embodiment, the persimmon tree multi-variety top grafting and head changing hybrid seed production method can obtain 7519-8162 hybrid seeds on 1 parent tree growing for 30-40 years each year, which is far higher than that of the traditional hybrid seed production method (367 seeds), and the seed production efficiency is improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (2)
1. A persimmon tree multi-variety top-grafting head-changing hybrid seed production method comprises the following steps:
1) taking the place where no male persimmon tree germplasm resources exist within 10-15 km of a square circle as a hybrid planting field;
2) selecting a persimmon tree without plant diseases and insect pests as a mother plant on the hybrid seed production land in the step 1);
3) taking different persimmon tree varieties which only bloom female flowers and do not bloom male flowers as female parents;
4) grafting the male parent and the female parent in the step 3) onto the stock plant in the step 2), and growing for 2-3 years to produce seed;
the female parent is one or more of Qingzhou small calyx, Spearmint persimmon, Kigelia japonica, Taian small batter, locomotive persimmon, Fuping jian persimmon, Maocheng persimmon, Boai Baoyue yellow, Mopan persimmon, Pingguan, Yangfeng, early autumn, Xinjin 20, Xianlang, black persimmon, oil creeper head, Japanese compass, lotus persimmon, fire crystal, September, flagpole top and cinnabar red;
the male parent is kaki, autumn or late autumn;
the male parent is grafted on the top end of the mother plant, and the female parent is grafted around the mother plant, so that at least one head is ensured to survive independently in the grafting process.
2. The hybrid seed production method according to claim 1, wherein the stock plant is a 30-40 year old persimmon tree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710048089.2A CN106818226B (en) | 2017-01-20 | 2017-01-20 | Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710048089.2A CN106818226B (en) | 2017-01-20 | 2017-01-20 | Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106818226A CN106818226A (en) | 2017-06-13 |
| CN106818226B true CN106818226B (en) | 2020-05-05 |
Family
ID=59119975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710048089.2A Active CN106818226B (en) | 2017-01-20 | 2017-01-20 | Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106818226B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108307835A (en) * | 2018-03-20 | 2018-07-24 | 云南省德宏热带农业科学研究所 | A kind of engrafting method of the more strain frangipanis of homophyletic |
| CN112772171A (en) * | 2021-02-08 | 2021-05-11 | 福建省农业科学院果树研究所 | Method for increasing number of waxberry hybrid progeny |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102939847A (en) * | 2012-11-29 | 2013-02-27 | 保山市映山红甜柿开发有限公司 | High yield cultivation technical method for multi-head top grafting of jiro persimmon on syzygium aromaticum |
| CN103081724A (en) * | 2013-01-15 | 2013-05-08 | 西北农林科技大学 | Persimmon tree grafting method |
| CN105191675A (en) * | 2015-11-03 | 2015-12-30 | 王玉保 | Multi-line crossing multi-fruit variety scenery tree and grafting method thereof |
| CN106171558B (en) * | 2016-07-21 | 2019-05-03 | 山东农业大学 | A kind of 8 × 9 apple multi items height obtain hybrid seed method |
-
2017
- 2017-01-20 CN CN201710048089.2A patent/CN106818226B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106818226A (en) | 2017-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111837791B (en) | Method for quickly establishing clonal seed orchard of castanopsis hystrix and application thereof | |
| CN110235776B (en) | Tomato sunlight greenhouse seed production method | |
| CN111480570A (en) | Crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome and application | |
| CN109566404B (en) | Method for producing hybrid seeds of two-season loofah in one year | |
| CN106818226B (en) | Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees | |
| CN113785741A (en) | Seed production method for pepper breeding | |
| Silva et al. | Advances in the propagation of Brazilian Cherry tree | |
| CN105075854A (en) | Breeding method for early-maturing and disease-resistant broccoli | |
| CN106171558B (en) | A kind of 8 × 9 apple multi items height obtain hybrid seed method | |
| Teaotia et al. | Flowering, Pollination, Fruit-Set and Fruit-Drop Studies in Ber (Zizyphus Mauritian A Lamak) II. Pollination Fruit-Set, Fruit Development and Fruit-Drop | |
| CN111296195B (en) | One-year three-crop fruiting cultivation method of red-meat pitaya | |
| CN110249929B (en) | Rapid propagation method for muskmelon germplasm resources | |
| CN108739364B (en) | Method for improving seed production efficiency of self-incompatible gramineous plant hybridization | |
| CN110338053B (en) | Breeding method of orange pagoda cauliflower hybrid | |
| CN107494252A (en) | A kind of method for improving eggplant hybrid seeding efficiency | |
| CN113575413A (en) | Breeding method of new variety of michelia figo | |
| CN108651272B (en) | Breeding method of peaches with low cold demand | |
| CN107466661B (en) | Topping and lodging-resistant cultivation method for flax for fiber or oil fiber | |
| CN111869446A (en) | Grafting cultivation method of wisteria | |
| CN104885927A (en) | Natural pollination hybridizing method with castration for longan | |
| CN101116419A (en) | Centipede grass cross-breeding method | |
| CN111296284A (en) | Efficient walnut crossbreeding method | |
| CN111264379A (en) | Banana cultivation method convenient for artificial pollination | |
| CN104770288A (en) | Making method of tea tree bonsai | |
| CN116250477B (en) | Method for cross breeding by using male sterile litchi |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |