CN111480570A - Crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome and application - Google Patents
Crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome and application Download PDFInfo
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- CN111480570A CN111480570A CN202010455704.3A CN202010455704A CN111480570A CN 111480570 A CN111480570 A CN 111480570A CN 202010455704 A CN202010455704 A CN 202010455704A CN 111480570 A CN111480570 A CN 111480570A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The invention relates to a method for crossbreeding between wild buckwheat rhizome and tartary buckwheat rhizome and application thereof, wherein tetraploid tartary buckwheat rhizome is used as a female parent, a diploid wild buckwheat rhizome compound plant doubling line and/or a naturally existing tetraploid wild buckwheat rhizome is used as a male parent for crossbreeding, and a crossbreeding variety obtained by adopting a crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome can live through winter in warmer regions in the south, thereby realizing asexual propagation production cultivation in one year; the breeding method of the invention not only utilizes the interspecific heterosis between the wild buckwheat and the tartary buckwheat, but also utilizes the heterosis between different wild buckwheat strains, further improves the perennial characteristic and the multiple regeneration capability of the interspecific hybrid of the wild buckwheat, and enables the branch cutting and the multiple cutting regeneration capability of hybrid plants to be powerful enough to support overwintering and multiple branch cutting propagation, thereby permanently keeping the heterosis of the hybrid and being widely popularized and applied in production.
Description
Technical Field
The invention relates to a plant breeding and propagating method, in particular to a crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome and application thereof.
Background
There are about 23 kinds of plants of the genus Fagopyrum, including annual cultivated species such as common buckwheat (also known as Fagopyrum esculentum) and tartary buckwheat (f.tataricum), and perennial species such as wild buckwheat (f.megasphatantium), wild buckwheat (f.pilus), and wild buckwheat (f.cymosus). The latter three perennial buckwheat species are difficult to distinguish by laymen due to their similar morphology, and are often referred to as wild buckwheat complex (f. The interspecific crossing of the buckwheat is difficult, and no example of successful crossing and utilization in production exists at present.
The heterosis utilization research of buckwheat is mainly on buckwheat. Gao Li Rong et al utilizes the characteristic of heterosis self-incompatibility of the flower columns of different plants of common buckwheat to cultivate common buckwheat inbred lines first, then utilizes 2 inbred lines to plant alternately, and carries out natural hybridization pollination between different flower type plants among the inbred lines to obtain about 50-80% of hybrid plants. This is a successful approach to partially exploit heterosis, which can increase yield by about 10-20%.
The use of hybrid crops is widely used mainly for rice, corn, sorghum and other major crops. The basic method is to cultivate male sterile line first and then to make natural hybridization between male sterile line and restoring line to produce hybrid seed. The major crop plants adopt the above-mentioned hybridization methods, mainly because of the lack of perennial characteristics. If the seeds have perennial characteristics, the seeds do not need to be produced every year. Can directly carry out asexual propagation such as cutting propagation and the like on the hybrid plants, and is popularized. Although no male sterile line is obtained from buckwheat, no hybrid buckwheat is produced by using the sterile line, but perennial buckwheat varieties exist in buckwheat plants, so that the characteristics of perennial buckwheat can be utilized to perform heterosis utilization and hybrid production, and production, popularization and application can be performed by asexual propagation. At present, no report is found on the buckwheat hybrid based on the perennial characteristics.
In view of this, this patent is filed.
Disclosure of Invention
Based on the problems, the inventor adopts tetraploid fagopyrum tataricum as a female parent, a diploid fagopyrum tataricum compound plant double line or a naturally existing tetraploid fagopyrum tataricum as a male parent to perform hybridization to cultivate fagopyrum tataricum and fagopyrum tataricum hybrid varieties, and the regeneration capacity of hybrids is greatly improved by utilizing the inheritance of perennial characteristics of perennial fagopyrum tataricum compound plants introduced by the hybridization, so that the hybrid plants can be subjected to propagation expansion and production utilization by adopting an asexual propagation method. The variety has the advantages of large grains, high flavone content, strong regeneration force after cutting and the like, can save seeding links, can improve the yield by more than 10 percent, and improves the quality of grains.
The technical scheme adopted by the invention is as follows:
a crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome, which comprises the following steps:
(1) selecting parents: tetraploid fagopyrum tataricum is used as a female parent, and a diploid fagopyrum tataricum compound plant doubling line and/or a naturally existing tetraploid fagopyrum tataricum is used as a male parent;
(2) castration and pollination: respectively cultivating the female parent and the male parent in the step (1) and adjusting the sowing period of the female parent to ensure that the flowering periods of the female parent and the male parent meet each other; castration is carried out on the female parent, pollen grains of the male parent are pollinated to flower columns of the female parent; after pollination, waiting for the seeds obtained by hybridization to be mature, harvesting to obtain hybrid seeds,
(3) carrying out thin sowing on the hybrid seeds obtained in the step (2) in the next sowing season to grow an amphidiploid interspecific hybrid plant (named as fagopyrum tataricum, F. tatari-cymosum), then harvesting seeds, carrying out thin sowing again, selecting plants which are early-maturing, non-dropping, thick, strong in regeneration force, full in seeds, good in seed form and high in yield from the later generations, and forming a good fagopyrum tataricum strain after multi-generation selective breeding stabilization;
(4) excellent cross breeding between strains: and (3) obtaining a plurality of excellent wild buckwheat lines by taking different tetraploid wild buckwheat as female parents, carrying out second sexual hybridization on the lines, and cultivating to obtain a wild buckwheat and wild buckwheat hybrid variety (named wild buckwheat hybrid). The different kinds of tetraploid fagopyrum tataricum are because many different diploid conventional fagopyrum tataricum naturally exist, and different kinds of tetraploid fagopyrum tataricum can be obtained by the method for obtaining the tetraploid fagopyrum tataricum in the step (1) of the invention and used as female parents. Furthermore, the male parent of the present invention includes a diploid line of Fagopyrum tataricum or the like and a naturally occurring tetraploid Fagopyrum tataricum, and forms various hybrids with the female parent of the present invention.
Further, the naturally occurring tetraploid wild buckwheat is some natural tetraploid type wild buckwheat distributed in Guizhou, Yunnan, Tibet, etc. The leaf, flower and fruit of the naturally occurring tetraploid wild buckwheat are small, the underground stem is mostly long and slender, most of the underground stem has underground transverse stem, and the tetraploid wild buckwheat is a tetraploid type in the wild buckwheat compound.
Further, in the step (1), the obtaining method of the tetraploid tartary buckwheat comprises the following steps: soaking tartary buckwheat seeds in 0.1-0.3 wt% colchicine water solution for 20-28 hours, and sowing and cultivating; from the seedling emergence to the two-leaf period, covering the stem tip of the plant with cotton, and dripping colchicine water solution on the cotton respectively 1 time in the morning and at night until the cotton is fully wet; removing cotton after 5-9 days, waiting for new buds at the treatment position to grow, and obtaining large-grained seeds, namely the tetraploid tartary buckwheat after the visible leaves are thickened and the flowers and fruits are grown to be fructified.
Further, in the step (1), the diploid wild buckwheat rhizome complex plant double line is obtained by: collecting wild buckwheat species in diploid wild buckwheat compound existing in the field, covering stem tips of the wild buckwheat plants with cotton when new buds begin in spring, and dripping 0.1-0.3 wt% colchicine aqueous solution on the cotton 1 time in the morning and at night each day until the wild buckwheat plants are fully wet; and removing the cotton after 5-9 days, waiting for new buds at the treatment position to emerge, and obtaining large-grained seeds, namely the diploid wild buckwheat rhizome compound plant double line, wherein the leaves are thickened and the flowers and fruits are enlarged.
The wild buckwheat variety in the diploid wild buckwheat compound is distributed in a large amount of diploid wild buckwheat compounds in southern provinces such as Guizhou province, Sichuan province and Yunnan province, leaves, flowers and fruits of the wild buckwheat variety are large, underground roots and stems are spherical, and the wild buckwheat variety generally does not transversely run under the ground.
Further, the colchicine aqueous solution was 0.2 wt%.
Further, in step (2), the castration operation is: and when the female parent plant blooms at the early stage, removing stamens when the anthers in the opened tartary buckwheat flowers are not cracked and pollen-dispersed at 8-11 points in the morning.
Further, in the step (2), the pollination operation is: and (3) applying the pollen grains of the just-scattered anther of the long-style short-stamen type just-bloomed flower of the male parent to the style of the castrated flower of the female parent tartary buckwheat.
Further, in the step (2), the emasculation and pollination are specifically: at the early flowering stage of the female parent plant, taking a magnifying glass at 8-11 points in the morning, removing stamens when the anther in the opened tartary buckwheat flower is not cracked and pollen-dispersed, and then coating pollen grains of the just pollen-dispersed anther of the just blossomed tartary buckwheat flower on the tartary buckwheat flower column.
Further, in the step (2), after pollination is finished, waiting for 25 days, and maturing and harvesting seeds obtained by hybridization. Since the plant grown from the hybrid seed is an amphidiploid interspecific hybrid (F.tatari-cymosus) of the tartary buckwheat and wild buckwheat compound plant, the plant morphology and growth development are between those of annual and perennial wild buckwheat compound plants, and the plant is easy to identify. The hybrid progeny has segregation such as seed falling, seed setting rate, branch thickness and number, long style short stamen or short style short stamen. Plants which are early-maturing, non-dropping, strong in regenerability, full in seeds, good in seed form and high in yield are selected from the offspring of the Fagopyrum tataricum, and after the plants are stably selected for multiple generations, excellent Fagopyrum tataricum strains which are early-maturing, high in selfing maturing rate, full in seeds and good in seed form are formed.
Further, in the step (3), the plants can be bred or utilized in production by adopting a branch cuttage or seed propagation mode, and the distance between the thin plants is more than 5 cm.
Further, in the step (4), the specific operation method of the cultivation is as follows: different excellent tartary buckwheat lines are obtained by taking different tetraploid tartary buckwheat as female parents, second sexual hybridization is carried out on the lines, combined hybrid plants with strong hybrid vigor, high yield and good quality are selected, and branch cuttage propagation is carried out to form hybrid varieties with consistent shapes.
The hybridization breeding method between wild buckwheat and tartary buckwheat is applied to breeding.
Furthermore, the application comprises the hybrid variety obtained by the hybrid breeding method between the wild buckwheat rhizome and the tartary buckwheat rhizome.
The inventor has used the method of the invention to obtain tetraploid tartary buckwheat female parents such as big tartary buckwheat No. 1, long black 4T tartary buckwheat, rice 55-4T and the like, tetraploid tartary buckwheat male parents such as red heart golden buckwheat No. 2, noble golden buckwheat No. 2 and the like, 5 interspecific hybrids (golden tartary buckwheat) and the like, and also carries out sexual hybridization for the 2 nd time between the filial generation non-falling seed strains to obtain extremely strong hybrid plants, obtains spring and autumn 2 seasons through branch propagation, and survives through winter to form a new species of noble golden tartary buckwheat No. 2.
Buckwheat species are difficult to be hybridized successfully, and the method can be successfully realized only by hybridizing tetraploid fagopyrum tataricum and tetraploid wild fagopyrum tataricum. The reason for this may be that tartary buckwheat is a self-pollinated crop, which can maintain its own genetic stability after long-term self-pollination, and the pollen recognition ability is degraded without strict pollen affinity recognition for a long time, so that interspecific hybridization can be realized relatively easily under the condition of artificial emasculation and pollination.
The problem of why the hybridization breeding of the tartary buckwheat is rarely carried out and the interspecific hybrid of the tartary buckwheat and the tartary buckwheat is rarely obtained is mainly because the tartary buckwheat flowers are extremely small and the artificial hybridization is very inconvenient. The artificial hybridization method used in the research is that when the tartary buckwheat flower just begins to open, the stamen of the flower is exposed and not cracked within tens of seconds, and at the moment, the stamen is most easily emasculated and pollinated under the assistance of a magnifying lens, namely, the artificial emasculation pollination method for the just-flowering flower. The technology ensures that the tartary buckwheat crossbreeding and interspecific crossbreeding can be successfully achieved.
The invention has the beneficial effects that:
the hybrid variety obtained by the hybrid breeding method between the wild buckwheat rhizome and the tartary buckwheat can live through winter in warmer areas in the south, thereby realizing asexual propagation production cultivation year after year. Namely, the cutting cultivation is carried out once, the spring and autumn of the current year can be continuously harvested, and under the condition of safe overwintering in the south, a low-investment ecological agricultural grain production mode of cultivating for 1 time year after year and harvesting for multiple seasons is realized.
The breeding method is used for cultivating interspecific hybrids of tartary buckwheat and wild buckwheat compound plants with strong perennial characteristics, the perennial characteristics of the wild buckwheat new species formed by common simple hybridization are between that of perennial wild buckwheat and annual tartary buckwheat, and most of the wild buckwheat new species are not enough to have enough wintering performance and strong repeated regeneration capacity. The cross breeding method of the invention not only utilizes the interspecific heterosis between the wild buckwheat and the tartary buckwheat, but also utilizes the cross between different wild buckwheat strains to generate the interspecific heterosis between strains, further improves the perennial characteristic and the multiple regeneration capability of the interspecific hybrid of the wild buckwheat, and ensures that the branch cutting and the multiple cutting regeneration capability of hybrid plants are strong enough to support overwintering and multiple branch cutting propagation, thereby permanently keeping the heterosis of the hybrid and being widely popularized and applied in production.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
Example 1: the embodiment provides a crossbreeding method and application of wild buckwheat and tartary buckwheat, and the method comprises the following specific steps:
(1) selecting parents: tetraploid tartary buckwheat is used as a female parent, and a diploid wild buckwheat compound plant doubling line or a naturally existing tetraploid wild buckwheat is used as a male parent;
(2) castration and pollination: respectively cultivating the female parent and the male parent in the step (1) and adjusting the sowing period of the female parent to ensure that the flowering periods of the female parent and the male parent meet each other; castration is carried out on the female parent, pollen grains of the male parent are pollinated to flower columns of the female parent; after pollination, waiting for the seeds obtained by hybridization to be mature, harvesting to obtain hybrid seeds,
(3) carrying out thin sowing on the hybrid seeds obtained in the step (2) in the next sowing season, wherein the inter-plant distance is 6cm, growing into amphidiploid interspecific hybrid plants, harvesting the seeds, carrying out thin sowing again, wherein the inter-plant distance is 6cm, selecting early-maturing, non-falling seeds, strong and strong regenerability, plump seeds, good seed form and high yield plants from the later generations, and carrying out multi-generation selective breeding to form excellent golden tartary buckwheat strains;
(4) excellent cross breeding between strains: and carrying out second sexual hybridization on the strains to obtain a plurality of excellent golden tartary buckwheat strains obtained by taking different tetraploid tartary buckwheat as female parents or different male parents, and breeding to obtain hybrid varieties among the golden tartary buckwheat strains.
Example 2: the embodiment provides a crossbreeding method and application of wild buckwheat and tartary buckwheat, and the method comprises the following specific steps:
(1) obtaining tetraploid tartary buckwheat of different tartary buckwheat varieties: soaking conventional tartary buckwheat seeds in 0.2% colchicine for 24 hours, and sowing and cultivating in a flowerpot; from seedling emergence to the two-leaf period, wrapping a small amount of cotton on the stem tip of the plant, and dripping 0.2% colchicine solution on the cotton respectively 1 time in the morning and at night until the cotton is fully moistened; removing cotton after about 1 week, waiting for new buds at the treatment position to emerge, and observing that the leaves are thickened, the flowers and the fruits are enlarged, and the fruity large-grained seeds are the tartary buckwheat autotetraploid; thereby, tetraploid strains of a plurality of different tartary buckwheat varieties can be cultivated, and female parents are prepared for interspecific hybrids of the tartary buckwheat;
(2) obtaining tetraploid wild buckwheat rhizome: a large amount of diploid wild buckwheat, namely wild buckwheat, exists in the wild of southern provinces such as Guizhou province, Sichuan province and Yunnan province; the method comprises the following steps of (1) covering a small amount of cotton on stem tips of conventional diploid wild buckwheat variety, namely wild buckwheat collection line plants, when new buds begin to sprout in spring, and dripping 0.2% colchicine solution on the cotton 1 time in the morning and evening each day until the cotton is fully moistened; removing the cotton after about 1 week, waiting for new buds to emerge at the treatment position, and showing that the leaves are thickened, the flowers and the fruits are enlarged, and the fruity large-grained seeds are the autotetraploid of the wild buckwheat rhizome. Meanwhile, some naturally occurring tetraploid types, namely tetraploid wild buckwheat, which are also distributed in Guizhou, Yunnan, Tibet and the like are adopted; thus obtaining a plurality of different wild buckwheat rhizome tetraploid strains, and preparing male parents for wild buckwheat rhizome interspecific hybrids;
(3) interspecific hybridization of tetraploid tartary buckwheat and tetraploid wild buckwheat: the tartary buckwheat homotetraploid and tetraploid wild buckwheat (long-style short stamen type plants) generated by the method are respectively used as a female parent and a male parent, the female parent and the male parent are cultivated in a flowerpot, and the sowing period of the female parent is adjusted to ensure that the two florescence meet. When the female parent plant blooms at the early stage, 8-11 points in the morning are provided with a magnifier, stamens of anthers in the opened tartary buckwheat flowers which are not cracked and shed pollen are removed, and pollen grains of the just-shed pollen anthers of the fresh tartary buckwheat flowers are coated on tartary buckwheat flower columns; interspecific hybridization is carried out in this way, and hundreds of flowers are pollinated; after 25 days, after the hybrid seeds are mature, harvesting the hybrid seeds; the plant grown from the hybrid seed is a diploid interspecific hybrid of tartary buckwheat and wild buckwheat (F.tatari-cymosum, golden tartary buckwheat), is between annual tartary buckwheat and perennial wild buckwheat in plant morphology and growth development, generally shows that the hybrid seed is self-fertile, strong in stalk, enhanced in lignification and developed in root system, is similar to the perennial wild buckwheat of a male parent, white in flower, short in style and self-fertile, and is larger than the wild buckwheat of the female parent, and the fruit has no groove and is different from the wild buckwheat of the female parent, so that the true hybrid seed is very easy to identify, and the progeny of the hybrid seed is separated into the falling seed property, the setting rate, the branch thickness and number, the long style short stamen or the short style short stamen; plants which are early-maturing, non-dropping, strong in regeneration force, full in seeds, good in seed form, high in yield and capable of achieving selfing of the same length of the flower column are selected from the plants, and after the plants are subjected to multi-generation selection stabilization, excellent golden tartary buckwheat strains which are early-maturing, high in selfing maturing rate, full in seeds and good in seed form are formed; by the same method, interspecific hybrid strains of different tartary buckwheat and different wild buckwheat can be obtained;
(4) hybridization between good lines of non-shedding grain of tartary buckwheat-tartary buckwheat interspecific hybrids (tartary buckwheat): hybridizing the obtained different Fagopyrum tataricum lines again, selecting a combined hybrid plant with strong heterosis, high yield and good quality, and performing branch cutting propagation to form a new hybrid variety, including a new variety of Fagopyrum tataricum No. 2;
under the condition of good temperature and humidity, the branches of the hybrid plants can automatically take root and survive and regenerate without being treated by auxin such as indolebutyric acid and the like;
(5) the branches of the hybrid plants are respectively subjected to direct cuttage rooting or are treated by auxin such as indolebutyric acid 75ppm and the like for several hours to 1 day, and then cuttage rooting is carried out, so that a group is formed by propagation, and the plant can live through the winter in a warmer area in the south, thereby realizing asexual propagation production cultivation in one year. Namely, the cutting cultivation is carried out once, the spring and autumn of the current year can be continuously harvested, and under the condition of safe overwintering in the south, a low-investment ecological agricultural grain production mode of cultivating for 1 time year after year and harvesting for multiple seasons is realized.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. A crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome is characterized by comprising the following steps:
(1) selecting parents: tetraploid fagopyrum tataricum is used as a female parent, and a diploid fagopyrum tataricum compound plant doubling line and/or a naturally existing tetraploid fagopyrum tataricum is used as a male parent;
(2) castration and pollination: respectively cultivating the female parent and the male parent in the step (1) and adjusting the sowing period of the female parent to ensure that the flowering periods of the female parent and the male parent meet each other; castration is carried out on the female parent, pollen grains of the male parent are pollinated to flower columns of the female parent; after pollination, waiting for the seeds obtained by hybridization to be mature, harvesting to obtain hybrid seeds,
(3) carrying out thin sowing on the hybrid seeds obtained in the step (2) in the next sowing season to form amphidiploid interspecific hybrid plants, then harvesting the seeds, carrying out thin sowing again, selecting plants which are early-maturing, non-dropping, strong in regeneration force, full in seeds, good in seed form and high in yield from the later generations, and carrying out multi-generation selective breeding to form excellent golden tartary buckwheat strains;
(4) excellent cross breeding between strains: and (3) obtaining a plurality of excellent wild buckwheat lines by taking different tetraploid wild buckwheat as female parents, carrying out second sexual hybridization on the lines, and cultivating to obtain a wild buckwheat and wild buckwheat hybrid variety.
2. The method for cross breeding between wild buckwheat rhizome and tartary buckwheat species as claimed in claim 1, wherein in step (1), said tetraploid tartary buckwheat is obtained by: soaking tartary buckwheat seeds in 0.1-0.3 wt% colchicine water solution for 20-28 hours, and sowing and cultivating; from the seedling emergence to the two-leaf period, covering the stem tip of the plant with cotton, and dripping the colchicine aqueous solution on the cotton respectively 1 time in the morning and at night until the cotton is fully wet; removing cotton after 5-9 days, waiting for new buds at the treatment position to grow, and obtaining large-grained seeds, namely the tetraploid tartary buckwheat after the visible leaves are thickened and the flowers and fruits are grown to be fructified.
3. The method for cross breeding between wild buckwheat rhizome and tartary buckwheat rhizome as claimed in claim 1, wherein in step (1), said diploid wild buckwheat rhizome complex plant double line is obtained by: collecting wild buckwheat species in diploid wild buckwheat compound existing in the field, covering stem tips of the wild buckwheat plants with cotton when new buds begin in spring, and dripping 0.1-0.3 wt% colchicine aqueous solution on the cotton 1 time in the morning and at night each day until the wild buckwheat plants are fully wet; and removing the cotton after 5-9 days, waiting for new buds at the treatment position to emerge, and obtaining large-grained seeds, namely the diploid wild buckwheat rhizome compound plant double line, wherein the leaves are thickened and the flowers and fruits are enlarged.
4. The method for cross breeding between wild buckwheat rhizome and tartary buckwheat rhizome as claimed in claim 2 or 3, wherein the colchicine water solution is 0.2 wt%.
5. The method for crossbreeding wild buckwheat rhizome and tartary buckwheat rhizome according to claim 1, wherein in the step (2), the castration operation comprises the following steps: and when the female parent plant blooms at the early stage, removing stamens when the anthers in the opened tartary buckwheat flowers are not cracked and pollen-dispersed at 8-11 points in the morning.
6. The method for cross breeding between wild buckwheat rhizome and tartary buckwheat rhizome as claimed in claim 1, wherein in step (2), said pollination is performed by: and (3) applying the pollen grains of the just-scattered anther of the long-style short-stamen type just-bloomed flower of the male parent to the style of the castrated flower of the female parent tartary buckwheat.
7. The method for cross breeding between wild buckwheat rhizome and tartary buckwheat rhizome as claimed in claim 1, wherein in step (2), after pollination is finished, waiting for 25 days, and maturing and harvesting seeds obtained by cross breeding.
8. The method for cross breeding between wild buckwheat rhizome and tartary buckwheat rhizome as claimed in claim 1, wherein in step (3), said plants can be bred or productively utilized by branch cutting or seed propagation, and the distance between said sparsely sowed plants is more than 5 cm.
9. The method for crossbreeding wild buckwheat rhizome and tartary buckwheat rhizome according to claim 1, wherein in the step (4), the specific operation method for cultivation comprises the following steps: different excellent tartary buckwheat lines are obtained by taking different tetraploid tartary buckwheat as female parents, the lines are subjected to second sexual hybridization, combined hybrid plants with strong hybrid vigor, high yield and good quality are selected, and branch cutting propagation is carried out to form hybrid varieties with consistent shapes.
10. Use of the crossbreeding method between wild buckwheat rhizome and tartary buckwheat rhizome as claimed in any one of claims 1 to 9 in breeding.
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| CN112167055A (en) * | 2020-11-05 | 2021-01-05 | 贵州师范大学 | Breeding and cultivation method of creeping tartary buckwheat variety growing in chlorophytum comosum shape |
| CN112385539A (en) * | 2020-11-05 | 2021-02-23 | 贵州师范大学 | Breeding method of early-maturing high-quality black rice buckwheat without cracking shells |
| CN114223533A (en) * | 2021-12-06 | 2022-03-25 | 吉林省白城市农业科学院(吉林省向日葵研究所) | Breeding method of high-yield and easy-to-peel tartary buckwheat variety |
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| CN112167055A (en) * | 2020-11-05 | 2021-01-05 | 贵州师范大学 | Breeding and cultivation method of creeping tartary buckwheat variety growing in chlorophytum comosum shape |
| CN112385539A (en) * | 2020-11-05 | 2021-02-23 | 贵州师范大学 | Breeding method of early-maturing high-quality black rice buckwheat without cracking shells |
| CN114223533A (en) * | 2021-12-06 | 2022-03-25 | 吉林省白城市农业科学院(吉林省向日葵研究所) | Breeding method of high-yield and easy-to-peel tartary buckwheat variety |
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