CN1284442C - Method for cultivating three-prism candied fruit novel variety by nuclear gene substitution - Google Patents
Method for cultivating three-prism candied fruit novel variety by nuclear gene substitution Download PDFInfo
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Abstract
The present invention relates to a method for cultivating a new variety of triangular-prism mamoncillo, which is obtained by a cultivation method of nuclear gene transposition and comprises the specific processes that wide hylocereus undatus is selected as a male sterile line, and triangular-prism mamoncillo with high quality is used as a maintenance line and a restoring line; the male pollen of the triangular-prism mamoncillo is pollinated on the gynoecium of the hylocereus undatus to cause the hylocereus undatus to grow out fruit, bred seeds are seeded, bred sterile plants are used as a new female parent, and then the male pollen of the triangular-prism mamoncillo is pollinated on the gynoecium of the new female parent; a sterile line is cultivated by three to a plurality of generations of hybridization, a homozygotic sterile line is used as scions, and the plants of the triangular-prism mamoncillo with high quality are used as stocks to carry out graftage and asexual propagation for cultivating a new variety with a developed root system, strong tillering force, thick and strong stipe, large fruit, more granules, good fruit quality and fresh taste; moreover, the new variety has the medicinal value of treating high blood fat, diabetes and aging resistance, and new varieties of colorful triangular-prism mamoncillo with orange red pulp, pink pulp, lilac pulp, etc. among yellow pulp, red pulp and white pulp can also be obtained.
Description
Technical field
The present invention relates to the method for a kind of triangular prism mamoncillo breed of variety, particularly cultivate the method for shades of colour triangular prism mamoncillo series new varieties with repeatedly pollination method.
Background technology
Originating in Mexican a kind of Cactaceae triangular prism belongs to mamoncillo (Hylocereus undatus Britt Rose) and claims sesame fruit again, the triangular prism mamoncillo, it is a kind of fruit of Cactaceae triangular prism platymiscium, belonging to Cactaceae column Indian fig subfamily triangular prism on botany classification belongs to, be the perennial succulents of half climber, the historical existing centuries of artificial cultivation.The pericarp of triangular prism mamoncillo takes on a red color, and pericarp length has red microscler scale, and pulp is evenly distributed with the seed of sesame shape, the color adularescent of pulp, yellow, redness, and fruit and stem be edible all.Because the triangular prism mamoncillo is nutritious, cultivation easily, thick-flavor, fresh and sweet tasty and refreshing, rich vitamins A1, B1, B2, B3 and C, contain calcium, zinc, iron, element such as magnesium and phosphorus, ediblely can play clearing liver, grow lung, effects such as ease constipation also have medical value, begin to introduce China's south plantation in recent years, but at present because improved seeds are less, have only the triangular prism mamoncillo of white flesh to sell on market, price is also higher, redness, the triangular prism mamoncillo output of yellow pulp is higher, the taste also triangular prism mamoncillo than white flesh is good, but the planting requirement condition is higher, introduces a fine variety back incompatibility local climate and soil condition, and kind easily morphs.
Because the triangular prism mamoncillo originates in tropical desert area, it is more drought-enduring plant, happiness is wet to be afraid of to waterlog, the happiness temperature is afraid of cold, happiness light is afraid of cloudy, adapt to hot dry climate, suitable growth temperature is 15--40 ℃, thereby can only cultivate in the torrid areas, China belongs to the temperate zone, the subtropical zone, has only southern Guangxi, Guangdong, Taiwan, Fujian, Hainan, areas such as Yunnan can be planted, but the time of yielding positive results shorten relatively, have only 3-4 month general every year, be subject to winter freezing dead when temperature drops to below 5 ℃, and be subjected to soil, and climatic influences, its growth rate is also slow than the original producton location, sugar content is not high yet, all between 8-12%, though medicinal nutritive value is suitable, the kind mouthfeel that has is also not so good for the triangular prism mamoncillo sugar content of most of white flesh.
Technology contents
Be located in the torrid zone in order to make the triangular prism mamoncillo that originates in area, Central America can adapt to China, each province, the south China plantation of subtropical zone, and overcome yielding poorly of original fruit, poor quality, sugar content is low, phase of bearing fruit of blooming is short, and the little situation that adapts to soil, the hillside growth of red and yellow clay, the inventor has applied for the patent of invention of " cultivation and the extraction method of natural pigments thereof of triangular prism mamoncillo new varieties " to China Intellectual Property Office in 09 month 10 days calendar year 2001,＜number of patent application〉01128447,＜notification number〉1341345,＜the day for announcing〉2002 years 03 month 27 days,＜inventor〉Huang Jisen, Song Kaiquan, cover together strange, come the light industry.This invention relates to a kind of method of the new varieties that cultivate plants and the method for extracting pigment from the fruit of this plant, be to originate in the triangular prism mamoncillo of South America as scion, employing be grown in the vitality of Chinese South China extremely strong belong to wild species platymiscium kind terrible lotus together as stock, after grafting, cultivate a kind of triangular prism mamoncillo new varieties one red dragon fruit, the Huanglong fruit, dragon fruit, this triangular prism mamoncillo new varieties have kept drought-enduring, heat-resisting quality, also showed cold-resistant, deficient resistance, also waterlogging new germplasm, its fast growth, nutritious, the pulp succulence, the content height of oligosaccharide, can suppress the too high and fat absorption of cholesterol, can also reducing human blood to the picked-up of glucose, have hypoglycemic, the effect of blood fat, this invention also discloses the method for extracting pigment from the fruit of this plant, be that redness or yellow triangular prism mamoncillo are cleaned removal scale and carpopodium, put it to chopping pulverizing in the cutter mill, extract redness or xanthein with water extraction or alcohol extracting method, perhaps water extraction adds alcohol extracting method extraction redness or xanthein.This patent of invention adopt the vitality that is grown in Chinese South China extremely strong belong to the wild species platymiscium together, after grafting, cultivate a kind of triangular prism mamoncillo new varieties---red dragon fruit, the Huanglong fruit, dragon fruit, and successfully make its plantation begin to enter the implementation phase, poor mountain area and tor area have been come into, become new projects of peasant programme, simultaneously, the test of really extracting natural colouring matter by Huanglong with the red dragon fruit of the red pulp of bright-colored and yellow pulp, obtained corresponding thick product, for a kind of better outlet has been found in the comprehensive exploitation of triangular prism mamoncillo.
But the inventor finds to adopt original grafting also to have many weak points, 1, grafting needs the professional of agriculture of higher level, because grasp badly, scion is difficult to maybe can not survive; 2, to note after the grafting kind terrible lotus young shoot below the scion is removed, otherwise can grow fruitless kind of terrible lotus; 3, technology grasps that red dragon fruit may cause degenerating when bad, we also find in practice, adopt same triangular prism mamoncillo scion and a kind terrible lotus stem bar, grafting method and identical management, the result who has is bigger, and the result who has is little, the fruit sweet taste is disunity also, the phenomenon that the result is a white flesh can occur after adopting red scion grafting sometimes, the cause that Here it is red dragon fruit and kind gene that terrible lotus is good fully do not show on same land for growing field crops.
The inventor is just on the basis of original 01128447 patent, research and exploration have been passed through again, adopt gene engineering research to produce the nuclear gene displacement and cultivate the triangular prism mamoncillo, the new method of particularly red dragon fruit, Huanglong's fruit new varieties, make the triangular prism mamoncillo quality that cultivates and growth performance is more stable, to the better adaptability of cold climate, it is also easier to cultivate.
Technical scheme of the present invention is:
The method applied in the present invention is a nuclear gene displacement cultivating method; it is to select the common nightblooming cereus (kind terrible lotus) of nature wild germplasm as the wild abortion type male sterile line; the high-quality triangular prism mamoncillo plant of South America is as maintenance line and recover system; the male pollen of triangular prism mamoncillo is awarded on the gynoecium of common nightblooming cereus; make common nightblooming cereus grow fruit; strictness is selected and is brought out the hybrid seed that recovers fertility; again with the planting seed that brings out; the sterile plant that selects is as new maternal; again the male pollen of triangular prism mamoncillo is awarded on the gynoecium of new female parent; hybridization through three to some generations; cultivate stable and pure male sterile line, and then with the male sterile line after isozygotying as scion, triangular prism mamoncillo homology germplasm plant makes stock; carry out grafting or vegetative propagation, the merit of male parent own is reappeared and express.Make by such hybridization that its gene is mutual, adding up obtains some quantitative character in next generation and break through the original proterties of parents, makes it the new proterties more favourable than parents occur.
The method of triangular prism mamoncillo new varieties is cultivated in above-described nuclear gene displacement, and the triangular prism mamoncillo kind that adopts male pollen is red or yellow, white flesh.If select the male parent of different colours at every turn, cultivating the triangular prism mamoncillo kind obtain at last has multiple color, may be between between yellow and red, the white, as orange red, pink, pale purple etc., can bring colourful triangular prism mamoncillo new varieties to market.
The method that triangular prism mamoncillo new varieties are cultivated in above-described nuclear gene displacement also comprises the flower synchronization control technology, and concrete grammar is:
1. grasp father and mother's flowering habit, best poplar bloassom phase originally.With requirement and the local meteorological data of female parent to factors such as temperature, humidity, sunlight, select best pollination to regulate implantation time season, and the plantation of male parent was sowed with the alternate time difference in batches, allow its flowering time produce and stagger, make this flower synchronization of father and mother.
2. with the dispensing different fertilizer Composition Control flower bud differentiation time, with the plain short poplar bloassom of potassium in advance, executing nitrogen delays to bloom and delays fully short control triangular prism mamoncillo flowering time, especially make full use of bloom about 20 days once characteristic of this fruit, make this flower synchronization of father and mother that more multimachine meeting be arranged.
3. the short control of temperature control flower synchronization: triangular prism mamoncillo flowering habit has a distinguished particularity, promptly working as triangular prism mamoncillo bud changes just, when floral organ has formed, as the temperature quenching, overcast and rainy continuously, during conditions such as long-term low temperature, bud can change into leaf bud, as the bud change just begun temperature recover after the reversible again bud that becomes of this bud, it is helpful that this characteristic is that temperature control is regulated flower synchronization.
4. the limiting temperature regulation and control can be regulated flower synchronization: the triangular prism mamoncillo has irregular dormancy characteristics, and the critical temperature of its dormancy is 10 ℃ and 38 ℃, and when being in these two critical temperatures, the triangular prism mamoncillo will enter resting state and stagnate and do not grow.And the temperature of separating dormancy is 12 ℃ and 35 ℃, can correct temperature control, can in a planned way regulate the purpose that this flower synchronization of father and mother reaches cross pollination.
From open source literature and above-mentioned background technology; we recognize the common nightblooming cereus tendril property plant that the Cactaceae triangular prism of China's South China's spontaneous growth belongs to (be commonly called as a kind terrible lotus, Entada phaseoloides, overlord spend etc.), are a kind of both heat-resisting, cold-resistant, waterlogging, anti-fertilizer, are not afraid of poor ridge, can adapt to the natural wild germplasm plant that various soil are grown.Common climbing on barren hill, tor mud seam, disconnected wall and cement wall held up growth, and other treetops of tens meters high are labeled also and can unsettledly be grown even, and growing way is vigorous and can leave gorgeous flower.The branch of common nightblooming cereus, flower local resident all have food custom, and have certain medicinal functions such as clearing heat and detoxicating, and Guangxi, many places, Guangdong also have the artificial planting flower of gathering to make soup diet good merchantable brand.The not long fruit yet common nightblooming cereus only blooms has the people of Basic of Biology to be easy to observe from its flower, though common nightblooming cereus is the male and female colors, should have the self-pollination condition.The pollen that yet the stamen development of common nightblooming cereus is undesired, can not produce function, no loose powder insemination ability.But gynoecium is grown normal, can accept normal foreign pollen and be fertilized and normally bear fruit, belong to the spontaneous mutation male sterile plant, the true difficulty of typical wild abortion type male sterile plant of this n cell cytoplasmic inheritance finds, this is for utilizing three-way crossbreeding to have important practical significance, for the present invention has laid the material base of male sterile line, can as mother of project this.
Triangular prism mamoncillo male and female colors, pistil is grown normal, can self-fertility, and can award male sterile line its pollen and can recover fertility, be the male parent strain that more satisfactory common nightblooming cereus homology is joined, the objective trait parent that can do crossbreeding uses.
The theoretical foundation that the method for triangular prism mamoncillo is cultivated in nuclear gene displacement of the present invention is that Yuan Longping hybrid rice theory is the three line method breeding method, be the effective way that obtains a large amount of first filial generation seeds promptly with manually selecting male sterile line, maintenance line and recovery system, this breeding be current the most frequently used, save trouble most, a kind of breeding approach of instant effect, it provides abundant material base for the hybrid assembly.
The mechanism of this technical scheme is that the stability shape that has according to crop eliminated by natural selection or artificial selection after long-term self-pollination, so harmful proterties seldom appears in the offspring, vitality also can not occur and significantly reduce phenomenon.But by the heterozygote of xenial hybridization, its harmful recessive gene is often covered by dominant allele, does not therefore show.Pure and stable new varieties to be obtained for this reason and the method for repeatedly backcrossing must be used, make plasmon and nuclear gene acting in conjunction, by genetic recombination harmful recessive character is fully come out, and through selecting to eliminate the new varieties of the merit of finally selecting and remain out.This method of repeatedly backcrossing is exactly the process of nuclear gene displacement.We use this mechanism and adopt the wild abortion type male sterile plant---and common nightblooming cereus does maternal and adopts Mexico's high-quality triangular prism mamoncillo to make male parent; by the cross pollination sexual hybridization; and repeatedly backcross; the cell nucleus gene of male parent is transferred to maternal cytoplasm; allow the cell nucleus gene of maternal cytoplasm and male parent interact, cause isozygotying and cultivate stable and pure male sterile line.And then with the male sterile line after isozygotying as scion, triangular prism mamoncillo homology germplasm plant makes stock, carries out grafting or vegetative propagation, the merit of male parent own is reappeared and expresses, strict again selecting brings out the hybrid seed that recovers fertility.
Below describe the preparation method of detail of the present invention and triangular prism mamoncillo new varieties in detail:
1, the acquisition of " genes of interest " is tasted the kind that South America triangular prism mamoncillo mouthfeel is good, output is high, proterties is good through selecting product, the column high-level cultivating is made in the land for growing field crops that is incorporated in corresponding ecotope, as the male parent gene plant, treating that it is finished changes over to when blooming reproduction period vegetative period, extract pollen and do cross pollination on maternal gynoecium, and obtain " genes of interest ".
2, choose suitable carrier; introducing " genes of interest " settles down best in quality; natural wild " common nightblooming cereus " stem bar that branch is sturdy is transplanted on the soil of silt content more than 50%; make high-level cultivating with the concrete column column,, form tree crown for sufficient water, fertilizer, intensive care; treat flower bud differentiation; floral organ forms, and gets paternal pollen during flower synchronization and carries out cross pollination and intersperse among on the female pistil, allows the maternal solid acquisition first filial generation seed that recovers.
3, the proterties of the amplification plant of " genes of interest " is determined by cell nucleus, and female parent is a cytoplasmic inheritance, therefore the cell nucleus of male sterile line is from the maintenance line male parent, so male sterile line has just formed similar substantially " twins " of proterties with corresponding maintenance line, but the intragentic reorganization part of this hybrid cell of father and mother is from male parent, and a part is from female parent.Therefore first filial generation paternal cell nuclear only accounts for 50%, will obtain pure, stable hybrid generation for this reason, must repeatedly backcross, and carries out the nuclear gene displacement, the cell nucleus of male parent is constantly transferred in the maternal cytoplasm gone, and reaches the amplification of " genes of interest ".
4, set up the recombinant DNA molecules vegetative propagation and express the proterties that " genes of interest " controlled
Make stock with the triangular prism mamoncillo breeding of introducing, the male sterile line female parent after isozygotying is done scion and is done vegetative propagation by grafting, allows recombination gene obtain expressing, and strictness is selected the good plant that can educate as new varieties.
5, cultivate applying of new varieties
Adopt cuttage or tissue cultivating method to breed out new varieties in a large number and application.
Description of drawings
Fig. 1 recovers gene seed selection schematic diagram.
Fig. 2 is a nuclear gene displacement schematic diagram.
As shown in Figure 1, maternal (common nightblooming cereus) sterile line F1 * male parent (triangular prism mamoncillo) have recovery capability can obtain sterile, Can give birth to separation, can educate not have and separate three kinds, only have to educate not have to separate to be used for cottage propagation and tissue culture propagation.
As shown in Figure 2, maternal (common nightblooming cereus) sterile line and male parent (triangular prism mamoncillo) are carried out the male parent base that for the first time hybridization obtains Because 50%, the male parent gene that for the second time hybridization obtains is 75%, the male parent gene that obtains through five hybridization is 98.4375%, Substantially reached requirement.
Compare with existing kind with the triangular prism mamoncillo new varieties that the method for karyogene displacement cultivation triangular prism mamoncillo of the present invention is cultivated, substantive distinguishing features that it is outstanding and significant progress are:
1, new varieties triangular prism mamoncillo has superior hereditary basis
Show that hybrid vigour is fully showed, improved viability, high-quality, lasting, high yield, how anti-:
1. Blooming is born fruit, and 8-10 can be bloomed and be born fruit in individual month after the hybrid plant transfer. The phase of bearing fruit is bright by not having May-November The aobvious branch season.
2. it is wide to plant the region: southern open country plantation, and greenhouse gardening can be adopted in the north, and the lowest limit temperature is 2 ℃, and is general Can both normal growth under 12-35 ℃ of temperature.
3. best in quality: edible, medicinal function and the proterties of triangular prism mamoncillo can intactly remain. By analysis check, the father This all technical data all can keep not morphing.
4. output height: carry then and kind full can bear fruit after 10 months, the first year, the output per mu yield was 500 kilograms, and next year reaches 1500-2000 Kilogram entered more than the output 5000-6000 kilogram in high term on the 3rd year, and lasting high yield reached more than 20 years.
5. fruit is big, setting percentage is high, more than the single fruit weight 400-500 gram, and 1000 grams that weigh most, fruit is thick-flavor, and is fresh and sweet Tasty and refreshing, pol 16%-20%.
2, the coordinated morphosis of triangular prism mamoncillo
The red dragon fruit of new varieties well developed root system, tillering ability is strong, cane is sturdy, and the big grain of fruit is many, and the setting percentage height has coordinates form preferably Structure.
1. well developed root system, root matter and root amount are with the obvious advantage to show that root is many, root is long, root is thick, rooting ability is strong, root system blazons.
2. tillering ability is strong: tiller early, long tiller is fast, stem and the difference in size of tillering are not obvious, the high yield group of formation neat and consistent Body structure.
3. thick, the wall thickness of stem, anti-fertilizer, stem growing way are vigorous, and the top phase is proper temperature, moisture, fertilizer, sun-drenched Branch extends more than the 2-2.5 centimetre every day under the part.
4. the how general every bar cement column length fruit 30-40 of the big grain of fruit individual more than, single fruit weight 0.5-1 kilogram.
3, new varieties triangular prism mamoncillo has vigorous physiological function
1. can form fast the tree crown that large tracts of land covers: so photosynthetic efficiency is higher, dry-matter accumulation is many, produces about 8 times of fruit per year, From blooming to maturation results, about 40 days of southern area, one batch of northern area about 45 days.
2. improving activity of root system is strong, and the ventilation of root is low, is beneficial to the conveying of oxygen, and that oxygen is supplied with is sufficient, strengthened the root breath function and Nutrient absorption capability, many to apical growing point rotation nutriment by nutrition organs, the tip that concentrates on branch of therefore bearing fruit is many, The top receives the prosperity of nutritional labeling ability, is one of physiologic factor that fruit is many, fruit is big, fruit is heavy.
In addition, the discovery of male sterile plant is lost in the open country, is used in crossbreeding and can have exempted a large amount of castration workers that the large tracts of land breeding is made Do, save manpower, reduce seed costs, guarantee hybrid seed purity, very favourable to heterotic utilization.
4, cultivate the triangular prism mamoncillo kind that obtains multiple color is arranged
The pulp colour of new varieties triangular prism mamoncillo may be between between yellow and red, the white, as orange red, pink, pale purple etc., Can bring rich in variety triangular prism mamoncillo new varieties to market.
5, new varieties triangular prism mamoncillo is the good fruit of a kind of nutrition
Adopting gene engineering research to produce the karyogene displacement and cultivate the triangular prism mamoncillo, is a kind of good fruit new varieties, and the medical values such as treatment high fat of blood, diabetes are arranged, and is in particular in:
1. contain the vegetalitas albumin---heavy metal detoxification agent in the human body:
Contain abundant phytalbumin in the Indian fig mamoncillo, after this phytalbumin is taken in the body by people, albumin will be engulfed heavy metal ion automatically, and promptly form a hard compound shell that is covered in the heavy metal surface, avoid heavy metal digesting and assimilating by digestive system in stomach in the food, blocked the infringement of heavy metal to other healthy cells, finally excrete quickly and smoothly, so albumin has played the detoxication of removing heavy metal in the human body via excretory organs.
2. contain more anthocyan---anti-oxidant, Green Tea Extract, anti-aging dose:
The triangular prism mamoncillo contains abundant anthocyan.This is a kind of antioxidant, and it is anti-oxidant, and to be better than carotin with power strong more than 10 times, and can received heat and the test of time not perishable.After people take in anthocyan, anthocyan can stop more than 70-75 hour at the blood in human body in pipe, give full play to its oxidation resistance, Green Tea Extract, anti-aging effect and with the auxilliary mutually more remarkable effect of the vitamin C of itself, the embrittlement of eater's skin surface is delayed, increase soft and moist, light, sliding tender, remarkably productive to beautifying skin.
It is to penetrate people's cerebrovascular disorder and improve brain cell and prevent the sex change ability that anthocyan also has a function the most special, thereby suppresses the generation of dementia.
3. be rich in water-soluble dietary fiber---auxiliary treating diabetes health-care agent:
The triangular prism mamoncillo contains abundant water solutable edible fiber composition, this material can absorb moisture content in a large number in vivo and make volume own increase 10-15 doubly, the cellulose that expands can form spawn and the interior food of combination, prolonged the holdup time of food the human digestive system, slow down hunger, this point helps fat-reducing.Because the water-soluble dietary fiber gel combines food, cause the time dilation that changes into sugar in the food by starch, therefore the speed that discharges sugar is slowed down, and then human body slows down to the infiltration rate of sugar, the sugar that deposits small intestine in is very few, can not occur sudden hyperglycaemia phenomenon in the blood, the also corresponding raising of the anti-sugared degree of human body makes the human body sugar transform the absorption mechanism sexual function and obtains to regulate automatically and balance.Reduce superfluous sugar infiltration excretory system and excreted phenomenon, therefore the triangular prism mamoncillo is remarkable to glycosuria patient's health-care effect, the volumetric expansion significantly of water-soluble dietary fiber in addition, the proportion of the ight soil of human body increases, accelerated drainage rate, noxious material shortened in the large intestine time of staying during excrement made, and alleviated and avoided large intestine generation enteritis and carcinogenic possibility, for defaecation ease constipation tool unique function.
4. the triangular prism mamoncillo is the environmentally friendly machine without agriculture chemical:
The Premeabilisation of cells of Indian fig mamoncillo forces down in general crops.The Premeabilisation of cells of general plant is pressed about about 18 atmospheric pressure, and the Premeabilisation of cells of Indian fig mamoncillo is pressed only 12 atmospheric pressure, therefore in the fertilising process, the inorganic fertilizer salinity surpasses 3 ‰, the negative reaction of reverse osmosis then may appear, therefore almost can not use chemical fertilizer in the whole process of cultivation triangular prism mamoncillo, its fertilizer source only relies on the supply of using of farmyard manure.
The triangular prism mamoncillo is not owing to there is leaf, the branch of triangular prism has the parcel of thin-layer cell and thick cured layer, therefore, plant can prevent that fly from stining insect bite, do not find that in the orchard of whole plantation specific insect is caused harm and disease takes place yet, without spraying pesticide, therefore, the pollution-free food typical species of current really nuisanceless, the environment-friendly type of this fruit.
5. the red dragon fruit of new varieties is pure natural redness, xanthein resource treasure-house
The red imperial arnotto skin red meat of new varieties contains abundant rose, and the color of Huanglong's fruit pigment is vivid, this natural colouring matter is nontoxic to have exploitation to be worth very much, if can process, can increase considerably the additional output value of triangular prism mamoncillo, enlarge economic benefit and social benefit.
Embodiment
Embodiment 1: the common nightblooming cereus of selecting the nature wild germplasm is as the wild abortion type male sterile line; the red pulp triangular prism of the high-quality of South America mamoncillo plant is as maintenance line and recover system; the male pollen of triangular prism mamoncillo is awarded on the gynoecium of common nightblooming cereus; make common nightblooming cereus grow fruit; strictness is selected and is brought out the hybrid seed that recovers fertility; again with the planting seed that brings out; the sterile plant that selects is as new maternal; again the male pollen of triangular prism mamoncillo is awarded on the gynoecium of new female parent; hybridization through 6 generations; cultivate stable and pure male sterile line; and then with the male sterile line after isozygotying as scion, triangular prism mamoncillo homology germplasm plant makes stock, carries out grafting and vegetative propagation; the merit of male parent own is reappeared and express, obtain the triangular prism mamoncillo new varieties of red pulp.The inventor is with this kind called after " the red dragon fruit of China ", " the red dragon fruit in Guangxi ".
Embodiment 2: the common nightblooming cereus of selecting the nature wild germplasm is as the wild abortion type male sterile line; the yellow pulp triangular prism of the high-quality of South America mamoncillo plant is as maintenance line and recover system; the male pollen of triangular prism mamoncillo is awarded on the gynoecium of common nightblooming cereus; make common nightblooming cereus grow fruit; strictness is selected and is brought out the hybrid seed that recovers fertility; again with the planting seed that brings out; the sterile plant that selects is as new maternal; again the male pollen of triangular prism mamoncillo is awarded on the gynoecium of new female parent; hybridization through 7 generations; cultivate stable and pure male sterile line; and then with the male sterile line after isozygotying as scion, triangular prism mamoncillo homology germplasm plant makes stock, carries out grafting and vegetative propagation; the merit of male parent own is reappeared and express, obtain the triangular prism mamoncillo new varieties of yellow pulp.The inventor is with this kind called after " Chinese Huanglong fruit " or " Guangxi Huanglong fruit ".
Embodiment 3: the common nightblooming cereus of selecting the nature wild germplasm is as the wild abortion type male sterile line; the high-quality of South America is red and yellow; the triangular prism mamoncillo plant of three kinds of pulp of white is as maintenance line and recover system; the male pollen of triangular prism mamoncillo is awarded on the gynoecium of common nightblooming cereus; make common nightblooming cereus grow fruit; strictness is selected and is brought out the hybrid seed that recovers fertility; with the planting seed that brings out; the sterile plant that selects is as new maternal; again the male pollen of triangular prism mamoncillo is awarded on the gynoecium of new female parent; it is (red or yellow that the male pollen color of each male parent that adopts is respectively different colours pulp; white) plant; hybridization through 7 generations; cultivate stable and pure male sterile line; and then with the male sterile line after isozygotying as scion; triangular prism mamoncillo homology germplasm plant makes stock, carries out grafting and vegetative propagation, the merit of male parent own is reappeared and expresses; obtained multiple color, as the triangular prism mamoncillo new varieties of pink or orange red pulp.The inventor is with this kind called after " middle brightness dragon fruit " or " the color dragon fruit in Guangxi ".
Claims (3)
1; a kind of method of cultivating triangular prism mamoncillo kind; comprise the common nightblooming cereus of choosing the nature wild germplasm and originate in the process of the triangular prism mamoncillo of South America as kind gene germplasm; it is characterized in that: the method that triangular prism mamoncillo kind adopts displacement to cultivate obtains; detailed process is to select nature wild germplasm common nightblooming cereus as the wild abortion type male sterile line; the triangular prism mamoncillo plant of South America is as maintenance line and recover system; the male pollen of triangular prism mamoncillo is awarded on the gynoecium of common nightblooming cereus; make common nightblooming cereus grow fruit; select the hybrid seed that recovers fertility; again with the planting seed that brings out; the sterile plant that selects is as new maternal; again the male pollen of triangular prism mamoncillo is awarded on the gynoecium of new female parent; hybridization through three to some generations; cultivate stable and pure male sterile line; and then with the male sterile line after isozygotying as scion, triangular prism mamoncillo homology germplasm plant makes stock, carries out grafting and vegetative propagation.
2, the method for cultivation triangular prism mamoncillo kind according to claim 1, it is characterized in that: cultivating process also comprises the flower synchronization control method, and concrete grammar is:
(1) with requirement and the local meteorological data of female parent to temperature, humidity, sunlight, select the season of pollinating, regulate implantation time, grasp father and mother's flowering habit, poplar bloassom phase originally, the plantation of male parent was sowed with the alternate time difference in batches;
(2) the dispensing different fertilizer Composition Control flower bud differentiation time;
(3) the short control of temperature control flower synchronization;
(4) the limiting temperature regulation and control can be regulated flower synchronization.
3, the method for cultivation triangular prism mamoncillo kind according to claim 1, the triangular prism mamoncillo kind that adopts male pollen are red or yellow, white flesh.
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| CN 03118561 CN1284442C (en) | 2003-01-26 | 2003-01-26 | Method for cultivating three-prism candied fruit novel variety by nuclear gene substitution |
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| CN 03118561 CN1284442C (en) | 2003-01-26 | 2003-01-26 | Method for cultivating three-prism candied fruit novel variety by nuclear gene substitution |
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| CN1433678A CN1433678A (en) | 2003-08-06 |
| CN1284442C true CN1284442C (en) | 2006-11-15 |
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| CN 03118561 Expired - Fee Related CN1284442C (en) | 2003-01-26 | 2003-01-26 | Method for cultivating three-prism candied fruit novel variety by nuclear gene substitution |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102823487B (en) * | 2012-09-10 | 2015-06-10 | 马保良 | Method for cultivating new grape variety Bairu grape |
| CN104956882B (en) * | 2015-06-19 | 2017-08-08 | 宋开泉 | The high-yield planting method of the red Hylocereus undatus of triangular prism and fruit |
| CN105532234B (en) * | 2015-12-25 | 2018-07-10 | 蒙奇同 | The breeding method of red heart Hylocereus undatus |
| CN110089364A (en) * | 2019-05-08 | 2019-08-06 | 福建省农业科学院果树研究所 | A method of shortening dragon fruit crossbreeding process |
| CN112088771A (en) * | 2020-10-10 | 2020-12-18 | 南京科森挤出装备有限公司 | Method for vacuum extracting plant juice and inputting another plant to cultivate new product and cross breeding |
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