CN108848989A - A kind of method for culturing seedlings of complete female balsam pear - Google Patents
A kind of method for culturing seedlings of complete female balsam pear Download PDFInfo
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- CN108848989A CN108848989A CN201810775437.0A CN201810775437A CN108848989A CN 108848989 A CN108848989 A CN 108848989A CN 201810775437 A CN201810775437 A CN 201810775437A CN 108848989 A CN108848989 A CN 108848989A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Developmental Biology & Embryology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of method for culturing seedlings of complete female balsam pear.The present invention is by grafting the complete resulting seedling of female plant scion, and the reason of because of stock, compared with complete female seed of bitter gourd seedling, grafting plant root wants much flourishing, and growing way wants much better than, and resistance also wants much better than, and pest and disease damage is few, produces that melon amount is big, and hybrid seed production yields are high.And the germination of the full female plant bud of balsam pear is very strong, generally often tuck in bud all and can extract out a side shoot, the bud of side shoot again can pumping side shoot, one plant of full female plant can theoretically adopt infinite number of side shoot and make scion, the seedling of " unlimited " mostly is grafted into, the full female plant strain of " unlimited " mostly is formed.In addition sponge gourd is bigger than balsam pear seed yield, and general sponge gourd is 300~500/melon, and balsam pear is 20~30/melon, and sponge gourd nursery is more much easier than balsam pear, and stock is readily available.The present invention can simply and effectively obtain a large amount of complete female balsam pears, and avoid silver nitrate pollution on the environment, and the yield of seed is also very high.
Description
Technical field
The present invention relates to vegetables seedling-raising technique field, especially a kind of method for culturing seedlings of complete female balsam pear.
Background technique
Balsam pear multivitamin rich in, especially Victoria C occupy it is melon first of, also containing the multi mineral of needed by human body
Matter etc., balsam pear fruit have good edible value；Simultaneously containing Momordica charantial, P albumen, fat eliminating element etc., there are also hypoglycemic, drops
Blood lipid and antibacterial anticancer and other effects have good medical value.The cultivated area and sales volume of balsam pear all increase quickly in recent years.It is bitter
Melon heterosis, hybrid vigor is stronger, and cenospecies is mainly used in balsam pear production at present, and hybridization seed of bitter gourd demand is big.Utilize complete female balsam pear
Make maternal progress hybrid seeding, is the method for the most easy saving of labor of balsam pear hybrid seeding.Complete female balsam pear is present in breeder and passes through
In the stable More female lines of the high homogenous that the methods of hybridization, inbreeding of more generation are brought out, usually and there is staminiferous plant to mix.It is strong female at this
There is the male flower of staminiferous plant to go to the female flower pollination of full female plant with it in system, plant a part that resulting seed is bred as be have staminiferous plant and
A part is full female plant.This More female lines are mainly also to breed in this way.Make the maternal production of hybrid seeds with such More female lines, needs
It artificially pulls out staminiferous plant or cuts the male flower of staminiferous plant, this can waste plant and spend labour.Another method is to use silver nitrate
Liquid induces full female plant female flower to generate hermaphrodite flower, and the male pollen of hermaphrodite flower is taken to pollinate to full female plant female flower, and gained seed is theoretically complete
Part is full female plant, but actually still having small part plant is to have staminiferous plant, and this silver nitrate solution induction generates the side of hermaphrodite flower
Method not only makes toxic silver nitrate polluted environment, while seed production is not also high.
Summary of the invention
The purpose of the present invention is：A kind of method for culturing seedlings of complete female balsam pear is provided, it can simply and effectively obtain a large amount of balsam pears
Full female plant while saving labour, will not pollute environment, and seed production is high.
The invention is realized in this way：A kind of method for culturing seedlings of complete female balsam pear, includes the following steps：
1) full female plant plantation：By the More female lines balsam pear culturing and transplanting seedlings with full female plant, density is 800~1500 plants/
667m2, full female plant pinching when plant grows to 1 meter or more, pulling out all staminiferous plants, and will be left behind, allow its lateral bud sprout at side
Branch；A liquid fertilizer is applied within 5~7 days every to the full female plant left, liquid fertilizer is compound potassium sulfate fertilizer liquid, and mass concentration is 2~3%, is applied
Dosage is 0.2~0.3L/ plants；Base manure is farmyard manure, and amount of application is 2000~5000Kg/667m2；Full female plant is pollinated without male flower,
Female flower is felt uneasy fruit；
2) stock nursery：By sponge gourd kind presoaking and germinating nursery, matrix is peat or self-control matrix；It is sufficiently rotten for making matrix by oneself
It is 1 that ripe animal dung and 7 years or more did not planted the rural area soil of melon crop in mass ratio：2~2.5 are formulated；It, will when nursery
One bunch planting of seed one after presoaking and germinating first carries out in the case where shading rate is 70~80% sunshade nets, makes the hypocotyl of sponge gourd seedling
When being elongated to 4~5cm, sunshade net is thrown off, allows seedling to grow under light illumination sturdy.
3) full female plant side shoot and scion production are adopted：Be 30~50cm when full female plant side shoot grows to length, slightly for 0.2~
When 0.25cm, side shoot production scion is adopted；When adopting side shoot, every is stayed 1~3 section on maternal plant；
4) it grafts：By scion grafting on stock, healing environment healing is put after grafting into；
5) seedling management：Grafting opens canopy film after healing 7~10 days under healing environment, but still lid sunshade net, according to
Weather condition and seedling growing state gradually throw off sunshade net；It is grown under light later, drenches 1% compound potassium sulfate fertilizer liquid 1~3
Secondary, every 5~7 days are primary, when seedling it is long to 10~15cm when transplanted.
Grafting side described in step 4) is the plumular axis method of plant propagation with cutting, specifically：To sponge gourd seedling hypocotyl ion leaf base 0.3~
At 0.5cm, with sponge gourd hypocotyl is less than and with the comparable bamboo stick of balsam pear scion thickness, the oblique of 30~45 degree of angles is cut on bamboo stick
Face, at a glance, for the bottom surface and sponge gourd embryo axial line for making eye at 30~45 degree of angles, when underthrust, does not wear out plumular axis opposite side, but bamboo to oblique cutting
Label have hand sense of touch when being inserted into opposite side；Then this eye is inserted into the scion made, makes scion axis and sponge gourd axis at 25-35 degree
Angle；Grafting is placed under healing environment later and is healed, sponge gourd cotyledon is cut after 7~10 days, scion is allowed to grow.
Grafting side described in step 4) is casing grafting, specifically：First the teleflex that diameter is 0.2~0.25cm is cut
The short tube of 0.6~0.8cm of growth is stand-by；Then to the oblique partial application at 1~2cm of ion leaf of stock hypocotyl, make section with anvil
The wooden plumular axis line is at 30~45 degree of angles；When grafting, first short tube is covered on stock, keeps stock upper end equal with sleeve upper end, then
Scion is inserted down, scion notch is made to be close together with stock notch；Put healing environment healing after grafting into.
Grafting side described in step 4) is pipe clamp grafting, specifically：It is oblique at 1~2cm of ion leaf to stock hypocotyl
Partial application makes section with stock plumular axis line at 30~45 degree of angles；Selecting diameter is that 0.2~0.25cm pipe clamp makees fixing clamp；When grafting,
First pipe clamp is clipped on stock, then inserts down scion, so that the both sides of clip is close to stock on one side, is close to scion on one side, allow
Scion notch is close together with stock notch, puts healing environment healing after grafting into.
The ambient humidity of the healing environment be saturation state, temperature be 25~30 DEG C, summer shading rate be 70~
80%.
The scion point scion with terminal bud and the scion without terminal bud, when being made without the scion of terminal bud, in petiole
Oblique partial application at lower 0.5~1cm, makes section with scion axis at 30~45 degree of angles, then 0.5~1cm is crosscutting above petiole
One knife cuts off branch, just forms a scion without terminal bud；With terminal bud scion production when, 3~4cm long branch most
Under oblique partial application at 0.5~1cm under a piece of petiole, make section with scion axis at 30~45 degree of angles.
The present invention is by grafting the complete resulting seedling of female plant scion, the reason of because of stock, compared with complete female seed of bitter gourd seedling, transfers
Seedlings picking plant root wants much flourishing, and growing way wants much better than, and resistance also wants much better than, and pest and disease damage is few, and production melon amount is big, hybrid seeding
Yield is high.And the germination of the full female plant bud of balsam pear is very strong, and a side shoot can be extracted out by generally often tucking in bud all, and the bud of side shoot can be taken out again
Raw side shoot, one plant of full female plant can theoretically adopt infinite number of side shoot production scion, graft the seedling at " unlimited " mostly, formed " unlimited "
More complete female plant.In addition sponge gourd is bigger than balsam pear seed yield, general sponge gourd be 300~500/melon, balsam pear be 20~30/
Melon, and sponge gourd nursery is more much easier than balsam pear, stock is readily available.The present invention can simply and effectively obtain a large amount of complete female hardships
Melon, and pollution on the environment is avoided, and the yield of seed is also very high.
Specific embodiment
The embodiment of the present invention 1：A kind of method for culturing seedlings of complete female balsam pear, includes the following steps：
1) full female plant plantation：At the beginning of Luodian county 1 month, by the strong female balsam pear QC2005 with full female plant, (QC2005 is expensive
State province fruit tree research through 12 generations be bred as the More female lines balsam pear with full female plant, be primarily used to balsam pear hybridize female parent) educate
Transplantation of seedlings, density are 866 plants/667m2, by normal water and fertilizer management, when plant being made to grow to 1~1.5m, staminiferous plant has been pulled out, and
The full female plant pinching that will be left behind allows its lateral bud to sprout into side shoot.Apply a liquid fertilizer within 5 days every to the full female plant left, liquid fertilizer is sulphur
Sour K composite fertilizer liquid, concentration 3%, amount of application are 0.2~0.3L/ plants.Base manure is farmyard manure, amount of application 3000Kg/
667m2.Male balsam pear not to have been planted in 1000 meters around the full female plant plantation, and full female plant is made to pollinate without male flower, female flower is felt uneasy fruit,
Plant growing way is prosperous, and the side shoot of sending is mostly again strong, adopts to make the more of scion and good.
2) stock nursery：By sponge gourd kind presoaking and germinating nursery, container hole tray, hole tray is 50 specifications, and matrix is self-control base
Matter.Self-control matrix is that abundant decomposed animal dung and 7 years or more did not planted the rural area soil of melon crop by 1:2 are formulated.It educates
Miao Shixian is to carry out on 70%~80% sunshade net in shading rate, when making the hypocotyl elongation of sponge gourd seedling to 4cm~5cm, is thrown off
Sunshade net allows seedling to grow under light illumination sturdy.
3) full female plant side shoot and scion production are adopted：When full female plant side shoot grow to length be 30~50cm, rugosity be 0.2~
Side shoot production scion can be adopted when 0.25cm, every is stayed 1~3 section on maternal plant when adopting side shoot, to sprout from the axillary bud of section from now on
Issue side shoot.Each branch under adopting can make 1~3 scion, and scion divides the scion with terminal bud and the scion without terminal bud, without
The oblique partial application at 0.5~1cm under petiole when the scion production of terminal bud, makes section with scion axis at 30~45 degree of angles, then
Branch is cut off in the horizontal partial application of 0.5~1cm above petiole, just forms a scion without terminal bud.Scion with terminal bud be
Oblique partial application at 0.5~1cm under a piece of petiole under most, makes section with scion axis at 30~45 degree of angles.Without the scion of terminal bud
With a piece of leaf, blade is excessive will suitably to cut a part.Scion length with terminal bud is 2~3cm.
4) it grafts：To sponge gourd seedling at 0.3~0.5cm of hypocotyl ion leaf base, be slightly less than sponge gourd hypocotyl and with
The comparable bamboo stick of balsam pear scion thickness (inclined-plane for having 30 degree or so on bamboo stick) oblique cutting at a glance, makes the bottom surface and sponge gourd plumular axis of eye
For heart line at 30~45 degree of angles, underthrust does not wear out plumular axis opposite side at the moment, but there is hand sense of touch in when bamboo stick is inserted into opposite side.Then it will make
This eye is inserted into scion, makes scion axis and sponge gourd axis at 30 degree of left and right corners.It will heal under grafting healing environment later, through 7
Sponge gourd cotyledon (neatly having cut sponge gourd growing point) is cut after~10 days, its scion is then allowed to grow.
5) seedling management：Grafting heals 7~10 days under healing environment can open canopy film, but still lid sunshade net, root
Sunshade net can be gradually thrown off according to weather condition and seedling growing state.It is grown under light later, drenches 1% compound potassium sulfate fertilizer liquid 2
Secondary, every 5~7 days primary.When seedling it is long to 10~15cm when can transplant.
Healing environment is outer cover sunshade net, the arched shed of inner lid plastic foil, lid or not lid sunshade net can be lacked when temperature is low, together
When can also be heated in arched shed, when temperature is high can the cooling of more lid sunshade nets, summer, general 70%~80% shading rate could.
The humidity of healing environment is saturation state, and temperature is 25~30 DEG C.
By embodiment 1, full female plant grafting and traditional seedling are subjected to hybrid seeding comparative test, 0.1 mu of plot area, two
Secondary repetition.As a result, grafting is than traditional seedling well developed root system, resistance, growing way is good, with its production of hybrid seeds and traditional seed density in planting phase
With in situation, i.e., density is 866 plants/667m2, more 12% of single melon grain weight than traditional seedling, single plant melon number more 20% totally mentions
It is high by 34%.
The embodiment of the present invention 2：A kind of method for culturing seedlings of complete female balsam pear, includes the following steps：
1) full female plant plantation：In Luodian county at the beginning of 1 month, by the strong female balsam pear XLQC with full female plant, (XLQC is Guizhou
Save fruit tree research institute through 17 generations be bred as the More female lines balsam pear with full female plant, be primarily used to balsam pear hybridize female parent) nursery
Transplanting, density are 1191 plants/667m2, by normal water and fertilizer management, when plant being made to grow to one meter or more, staminiferous plant has been pulled out, and
The full female plant pinching that will be left behind allows its lateral bud to sprout into side shoot.Apply a liquid fertilizer within 7 days every to the full female plant left, liquid fertilizer is sulphur
Sour K composite fertilizer liquid, concentration 2%, amount of application are 0.2~0.3L/ plants.Base manure is farmyard manure, amount of application 5000Kg/
667m2.Male balsam pear not to have been planted in 1000 meters around the full female plant plantation, and full female plant is made to pollinate without male flower, female flower is felt uneasy fruit,
Plant growing way is prosperous, and the side shoot of sending is mostly again strong, and the scion adopted is mostly again good.
2) stock nursery：By sponge gourd kind presoaking and germinating nursery, container hole tray, hole tray is 50 specifications, and matrix is in the market
The peat soil of purchase.When nursery first shading rate be 70%~80% sunshade net on carry out, make the hypocotyl elongation of sponge gourd seedling to 4cm
When~5cm, sunshade net is thrown off, allows seedling to grow under light illumination sturdy.
3) full female plant side shoot and scion production are adopted：When full female plant side shoot grow to length be 30~50cm, rugosity be 0.2~
Side shoot production scion can be adopted when 0.25cm, every is stayed 1~3 section on maternal plant when adopting side shoot, to sprout from the axillary bud of section from now on
Issue side shoot.Each branch under adopting can make 1~3 scion, and scion divides the scion with terminal bud and the scion without terminal bud, without
The oblique partial application at 0.5~1cm under petiole when the scion production of terminal bud, makes section with scion axis at 30~45 degree of angles, then
Branch is cut off in the horizontal partial application of 0.5~1cm above petiole, just forms a scion without terminal bud.Scion with terminal bud be
Oblique partial application at 0.5~1cm under a piece of petiole under most, makes section with scion axis at 30~45 degree of angles.
4) it grafts：To the oblique partial application of stock, make section with stock plumular axis line at 30~45 degree of angles.By diameter be 0.2~
The teleflex of 0.25cm is cut into the short tube of long 0.6~0.8cm.First by pipe sleeve on stock when grafting, make stock upper end and casing
Upper end is equal, then inserts down scion, and scion notch is made to be close together with stock notch.Put healing ring after grafting into
Border.Healing environment is the plastic canopy for being stamped sunshade net outside, and humidity is saturated mode, is cured under the healing environment that temperature is 25~35 DEG C
It closes.
5) seedling management：Grafting heals 7~10 days under healing environment can open canopy film, but still lid sunshade net, root
Sunshade net can be gradually thrown off according to weather condition and seedling growing state.It is grown under light later, drenches compound fertilizer solution 3 times of 1%, every 5
It is primary.When seedling it is long to 10~15cm when transplant.
Healing environment is outer cover sunshade net, the arched shed of inner lid plastic foil, lid or not lid sunshade net can be lacked when temperature is low, together
When can also be heated in arched shed, when temperature is high can the cooling of more lid sunshade nets, summer, general 70%~80% shading rate could.
The humidity of healing environment is saturation state, and temperature is 25~30 DEG C.
By embodiment 2, full female plant grafting and traditional seedling are subjected to hybrid seeding comparative test, 0.1 mu of plot area, two
Secondary repetition.As a result, grafting is than traditional seedling well developed root system, resistance, growing way is good, identical as traditional seed density with its production of hybrid seeds
When, i.e. 1191 plants/667m2, single melon grain weight is 12% more than traditional seedling, and single plant melon number more 23% is overall to improve 35%；Grafting
At 1.2 times of thin planting, i.e. 993 plants/667m2, single melon grain weight is 11% more than traditional seedling, and single plant melon number more 52% is overall to improve
38%.
Of the present invention to be not limited to embodiment described in specific embodiment, those skilled in the art are according to the present invention
Technical solution obtain other embodiments, also belong to the scope of the technical innovation of the present invention.Obvious those skilled in the art
Various changes and modifications can be made to the invention by member without departing from the spirit and scope of the present invention.If in this way, of the invention
These modifications and variations belong within the scope of the claims in the present invention and its equivalent technologies, then the present invention is also intended to encompass these changes
Including modification.
Claims (6)
1. a kind of method for culturing seedlings of complete female balsam pear, it is characterised in that：Include the following steps：
1) full female plant plantation：By the More female lines balsam pear culturing and transplanting seedlings with full female plant, density is 800~1500 plants/667m2, wait plant
When strain grows to 1 meter or more, the full female plant pinching pulling out all staminiferous plants, and will be left behind allows its lateral bud to sprout into side shoot；To what is left
Full female plant applies a liquid fertilizer for every 5~7 days, and liquid fertilizer is compound potassium sulfate fertilizer liquid, and mass concentration is 2~3%, and amount of application is 0.2~
0.3L/ plants；Base manure is farmyard manure, and amount of application is 2000~5000Kg/667m2；Full female plant is pollinated without male flower, and female flower is felt uneasy
Fruit；
2) stock nursery：By sponge gourd kind presoaking and germinating nursery, matrix is peat or self-control matrix；It is sufficiently decomposed for making matrix by oneself
It is 1 that animal dung and 7 years or more did not planted the rural area soil of melon crop in mass ratio：2~2.5 are formulated；When nursery, it will soak seed
One bunch planting of seed one after vernalization first carries out in the case where shading rate is 70~80% sunshade nets, makes the hypocotyl elongation of sponge gourd seedling
When to 4~5cm, sunshade net is thrown off, allows seedling to grow under light illumination sturdy；
3) full female plant side shoot and scion production are adopted：It is 30~50cm when full female plant side shoot grows to length, is slightly 0.2~0.25cm
When, adopt side shoot production scion；When adopting side shoot, every is stayed 1~3 section on maternal plant；
4) it grafts：By scion grafting on stock, healing environment healing is put after grafting into；
5) seedling management：Grafting opens canopy film after healing 7~10 days under healing environment, but still lid sunshade net, according to weather
Situation and seedling growing state gradually throw off sunshade net；It is grown under light later, drenches compound potassium sulfate fertilizer liquid 1~3 time of 1%, every 5
~7 days are primary, when seedling it is long to 10~15cm when transplanted.
2. the method for culturing seedlings of complete female balsam pear according to claim 1, it is characterised in that：Engrafting method embryo described in step 4)
The axis method of plant propagation with cutting, specifically：To sponge gourd seedling at 0.3~0.5cm of hypocotyl ion leaf base, with less than sponge gourd hypocotyl and with hardship
The comparable bamboo stick of melon scion thickness, the inclined-plane at 30~45 degree of angles is cut on bamboo stick, and oblique cutting at a glance, makes the bottom surface and sponge gourd embryo of eye
For axial line at 30~45 degree of angles, when underthrust, does not wear out plumular axis opposite side, but there is hand sense of touch in when bamboo stick is inserted into opposite side；Then it will make
This eye is inserted into scion, makes scion axis and sponge gourd axis at 25-35 degree angle；Grafting is placed under healing environment later and is healed,
Sponge gourd cotyledon is cut after 7~10 days, and scion is allowed to grow.
3. the method for culturing seedlings of complete female balsam pear according to claim 1, it is characterised in that：Engrafting method set described in step 4)
Pipe grafting, specifically：The short tube that the teleflex that diameter is 0.2~0.25cm is first cut into long 0.6~0.8cm is stand-by；Then
To the oblique partial application at 1~2cm of ion leaf of stock hypocotyl, make section with stock plumular axis line at 30~45 degree of angles；When grafting, first
Short tube is covered on stock, keeps stock upper end equal with sleeve upper end, then inserts down scion, cut scion notch with stock
It is tight-lipped to stick together；Put healing environment healing after grafting into.
4. the method for culturing seedlings of complete female balsam pear according to claim 1, it is characterised in that：Engrafting method pipe described in step 4)
Grafting is pressed from both sides, specifically：To the oblique partial application at 1~2cm of ion leaf of stock hypocotyl, make section with stock plumular axis line at 30~
45 degree of angles；Selecting diameter is that 0.2~0.25cm pipe clamp makees fixing clamp；When grafting, first pipe clamp is clipped on stock, then inserts scion
Go down, so that the both sides of clip is close to stock on one side, be close to scion on one side, allows scion notch to be close together with stock notch, transfer
Put healing environment healing after connecting into.
5. the method for culturing seedlings of complete female balsam pear described in -4 any one claims according to claim 1, it is characterised in that：It is described
Healing environment ambient humidity be saturation state, temperature be 25~30 DEG C, summer shading rate be 70~80%.
6. the method for culturing seedlings of complete female balsam pear according to claim 1, it is characterised in that：The scion point connecing with terminal bud
Fringe and scion without terminal bud, when making without the scion of terminal bud, oblique partial application at 0.5~1cm, makes section with connecing under petiole
Cob line is at 30~45 degree of angles, and then branch is cut off in the horizontal partial application of 0.5~1cm above petiole, just forms one without terminal bud
Scion；When scion production with terminal bud, 3~4cm long branch most under oblique partial application at 0.5~1cm under a piece of petiole,
Make section with scion axis at 30~45 degree of angles.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357516A (en) * | 2020-03-06 | 2020-07-03 | 光泽县富民蔬菜专业合作社 | Method for high-position grafting of bitter gourds |
| CN114097460A (en) * | 2021-12-15 | 2022-03-01 | 金华市农业科学研究院(浙江省农业机械研究院) | Method for controlling excessive growth of bitter gourd heterogenous grafted seedlings by using growth retardant |
| CN116671440A (en) * | 2023-06-20 | 2023-09-01 | 江苏农林职业技术学院 | In-vitro regeneration method of luffa hypocotyl and preparation of culture medium |
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| CN107509536A (en) * | 2017-10-18 | 2017-12-26 | 广州市农业科学研究院 | A kind of outer micro-grafting method of test tube of balsam pear tissue-cultured seedling |
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| CN111357516A (en) * | 2020-03-06 | 2020-07-03 | 光泽县富民蔬菜专业合作社 | Method for high-position grafting of bitter gourds |
| CN114097460A (en) * | 2021-12-15 | 2022-03-01 | 金华市农业科学研究院(浙江省农业机械研究院) | Method for controlling excessive growth of bitter gourd heterogenous grafted seedlings by using growth retardant |
| CN114097460B (en) * | 2021-12-15 | 2022-10-14 | 金华市农业科学研究院(浙江省农业机械研究院) | Method for controlling excessive growth of bitter gourd heterogenous grafted seedlings by using growth retardant |
| CN116671440A (en) * | 2023-06-20 | 2023-09-01 | 江苏农林职业技术学院 | In-vitro regeneration method of luffa hypocotyl and preparation of culture medium |
| CN116671440B (en) * | 2023-06-20 | 2024-05-03 | 江苏农林职业技术学院 | In-vitro regeneration method of luffa hypocotyl and preparation of culture medium |
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