CN102696370A - Twig cutting method of Lycium Chinense Mill. of variety Mengqi No.1 - Google Patents

Twig cutting method of Lycium Chinense Mill. of variety Mengqi No.1 Download PDF

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Publication number
CN102696370A
CN102696370A CN2012101598526A CN201210159852A CN102696370A CN 102696370 A CN102696370 A CN 102696370A CN 2012101598526 A CN2012101598526 A CN 2012101598526A CN 201210159852 A CN201210159852 A CN 201210159852A CN 102696370 A CN102696370 A CN 102696370A
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fringe
composition
root
cuttage
matrimony vine
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Inventor
王建平
秦海峰
郭喜平
温晓明
胡晓红
钟延平
鲍红春
张颖
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INNER MONGOLIA AUTONOMOUS REGION HORTICULTURAL INSTITUTE
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INNER MONGOLIA AUTONOMOUS REGION HORTICULTURAL INSTITUTE
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Abstract

The invention discloses a twig cutting method of Lycium Chinense Mill. of variety Mengqi No.1. The method comprises the steps of preparing branches Lycium Chinense Mill. of variety Mengqi No.1 into cutting woods; treating the cutting woods with a rooting composition to obtain treated cutting woods; and performing cuttage and seedling raising on the treated cutting woods. According to experiments provided by the invention, the twig cutting technology of Lycium Chinense Mill. of variety Mengqi No. 1 is systematically researched, and guidance for large-area popularized planting of Lycium Chinense Mill. of variety Mengqi No.1 is provided.

Description

No. 1 matrimony vine epicormic branch cuttage of a kind of illiteracy Qi method
Technical field
The present invention relates to biological technical field, relate in particular to No. 1 matrimony vine epicormic branch cuttage of a kind of illiteracy Qi method.
Background technology
China matrimony vine industry is occupied advantageous status in the world, the matrimony vine Products Development, the adjustment of masses' food configuration and consumption habit, the range of application of matrimony vine is more extensive, domestic and international demand increases day by day, in recent years matrimony vine supply falls short of demand.But the existing matrimony vine kind of China is very limited, is main with the lycium barbarum series of products mainly, and this can not satisfy the demand in market far away.Therefore, some high yields of seed selection, big, the high-quality of fruit are suitablely promoted the good matrimony vine kind of tailored version of development and are expanded that numerous to go out a large amount of good matrimony vine seedlings particularly important.
" cover Qi l number " matrimony vine is a kind of matrimony vine new varieties that the independent research of Inner Mongol academy of agricultural sciences horticulture institute selects, and recognizes fruit with illiteracy in 2005 to obtain kinds for No. 2005001 and assert certificates, and the l0 month in 2005 was carried out the achievement registration, and registration number is NK-20050102 [87].Matrimony vine has good characteristics such as fruit is big, output height " to cover Qi l number ".Supply falls short of demand for the matrimony vine seedling " to cover Qi l number " in river bend matrimony vine main producing region, large-area popularizing planting, is badly in need of the complete quick propagating technology of a cover.
Summary of the invention
The purpose of this invention is to provide a kind of matrimony vine epicormic branch cuttage method.
Method provided by the invention comprises the steps:
1) branch of clip matrimony vine tree is as inserting fringe;
2) with said slotting fringe with the compositions-treated of taking root, obtain handling the back and insert fringe;
3) inserted the fringe cuttage, grow seedlings in said processing back, realize the matrimony vine epicormic branch cuttage.
The step 2 of said method) in, the said composition of taking root is by solute and solvent composition, and said solute is made up of 2 kinds of materials with following mass ratio: naa: indolebutyric acid is 1:1.
In the said method, said solvent is a water;
Said naa and the said indolebutyric acid final concentration in the said composition of taking root is 100mg/kg-200mg/kg, and said naa and the said indolebutyric acid final concentration in the said composition of taking root all is specially 200mg/kg.
In the said method, said use is taken root compositions-treated for said slotting fringe base portion is picked the said composition of taking root.
In the said method, in the step 1), the rugosity of said slotting fringe is 0.2cm-0.4cm, and the rugosity of said slotting fringe is specially 0.3cm;
Said slotting fringe is a semi-lignified;
Lobe numbers on the said slotting fringe is 3-4, and the lobe numbers on the said slotting fringe is specially 3.
The branch of above-mentioned clip plant comprises the steps: that as slotting fringe the last annual spray of clip matrimony vine tree obtains the segment spray of 10-15cm; The upper end of this segment spray is a flat nick; Apart from terminal bud 2.5-3 centimetre; Lower cut is cut into the shape of a hoof, and apart from 0.5-1 centimetre of end bud side-lower, otch is level and smooth; The lower blade of this segment spray is all extractd, only stay and go up the 3-6 sheet most, and the base portion of this segment spray is put into clear water preserve and to obtain inserting fringe.
In the said method, in the step 3), said temperature of growing seedlings is 20 ℃-35 ℃; Said temperature of growing seedlings is specially 25 ℃-30 ℃; Said humidity of growing seedlings is 85%-95%; Said humidity of growing seedlings is specially 90%-95%.
Above-mentioned humidity is relative moisture, and relative moisture is meant the percent value of saturated steam density under actual contained water-vapour density of air and the equality of temperature.
The kind of above-mentioned matrimony vine is specially covers Qi No. 1.
Another object of the present invention provides a kind of composition of taking root.
The composition of taking root provided by the invention, by solute and solvent composition, said solute is made up of 2 kinds of materials with following mass ratio: naa: indolebutyric acid is 1:1.
In the above-mentioned composition, said solvent is a water.
In the above-mentioned composition, said naa and the said indolebutyric acid final concentration in the said composition of taking root is 100mg/kg-200mg/kg, and said naa and the said indolebutyric acid final concentration in the said composition of taking root all is specially 200mg/kg.
Above-mentioned composition is at the plant epicormic branch cuttage or promote that the application during spray is taken root in the plant cuttage also is the scope that the present invention protects; In the above-mentioned application, said plant is specially matrimony vine, and the kind of above-mentioned matrimony vine is specially covers Qi No. 1.
Experiment of the present invention proves; The present invention has carried out systematic research to the epicormic branch cuttage technology of " covering Qi No. 1 " matrimony vine; Utilize the root-growing agent of naa and earthworm diindyl butyric acid combination, when " covering Qi l number " matrimony vine epicormic branch cuttage, should select to insert the fringe rugosity is the semi-wooden scion of 0.2~0.4cm, and keeps 3~4 leaves; The optimum temperature of while seedling cultivation greenhouse is about 30 ℃, and humidity preferably remains between 95%~90%; Minimum humidity can not be lower than 80%, can improve survival rate, for " covering Qi l number " matrimony vine large tracts of land popularizing planting provides guidance.
Embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Overview experimental field among the following embodiment: this test is carried out in Inner Mongol gardening institute test site, and physical features is smooth, and soil property is a sandy loam, and the soil-ripening degree is high; Fertile soil, irrigation and drainage are convenient, and illumination is sufficient, and soil salt content is below 0.2%; Weeds are few, and damage by disease and insect is less, are easy to management.
Embodiment 1, No. 1 matrimony vine epicormic branch cuttage of illiteracy Qi method condition are groped
One, different root-growing agent prescriptions are handled " covering Qi l number " matrimony vine and are inserted the influence of fringe to the cuttage plant percent
1, cuttage
1), cutting bed is prepared
The cuttage seedbed be selected in Inner Mongol gardening experimental field.Soil property is a sandy loam, and irrigation and drainage are convenient, and leveling is ploughed deeply in former soil, make high 20cm, wide 1m, and the high bed of long 5m applies the sheep excrement 500kg/667m that becomes thoroughly decomposed then 2, covering the thick and river sand that mixed with 500 times of carbendazim of 5 ㎝ on the bed again, bed surface is paved, and waters primary water after mixing thoroughly deeply, only waters the bed surface sand bed, does not irrigate following soil, treats that bed surface does huskyly when diffusing, can carry out cuttage.The high 1.7m of seedling cultivation greenhouse, long 50 meters, wide 8 meters, the sunshade net that 50 ﹪ are taken in the outside is done and is shaded.
2), insert the preparation of fringe
Cutting selects for use on " cover Qi l number " matrimony vine (available from Inner Mongol gardening institute) good elite stand of new varieties annual spray as growing seedlings material; Choose early June when the new spray major part of extracting out reaches semi-lignified, branch is cut into the segment of 10-15cm, the upper end is a flat nick; Apart from terminal bud 2.5-3 centimetre; Lower cut is cut into the shape of a hoof, and apart from 0.5-1 centimetre of end bud side-lower, otch is level and smooth.Lower blade is all extractd, only stay and go up the 3-6 sheet most, base portion is put into clear water and is preserved, and obtains inserting fringe.
3), cuttage pre-treatment
Before the cuttage, earlier with the punching of punching nail-plate, nail rugosity 0.8cm, length 3cm, seeding row spacing are 6cm * 8cm, cuttage degree of depth 3cm.Be not advisable with jam-packed between seedling, water permeable.
4), different root-growing agent prescriptions are handled the back cuttage
Root-growing agent disposes following 4 kinds with root-growing agent naa NAA commonly used and indolebutyric acid IBA:
1. 100mg/kg naa (NAA) aqueous solution, 2. 100mg/kg indolebutyric acid (IBA) aqueous solution, 3. contain 100mg/kg naa (NAA) and 100mg/kg indolebutyric acid (IBA) solution (solvent is a water), 4. contain 200mg/kg naa (NAA) and 200mg/kg indolebutyric acid (IBA) solution (solvent is a water);
Above-mentioned 4 kinds of root-growing agent solution that prepare are mixed pasty state with talcum powder (also can be immersed in the root-growing agent solution inserting the fringe base portion, soak the 30min time, can reach same effect.) carry out following cuttage processing, to establish 4 experiments altogether and handle and a control treatment, each handles 100 strains, repeats 3 times:
C1 tests processing: with above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 100mg/kg NAA preparation;
C2 tests processing: with above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 100mg/kg IBA preparation;
C3 tests processing: with above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 100mg/kg NAA+100mg/kg IBA preparation;
C4 tests processing: with above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 200mg/kg NAA+200mg/kg IBA preparation;
CK control treatment: with above-mentioned 2) the slotting fringe for preparing directly inserts in the sand in seedbed.
5), seedbed management
After cuttage was handled, temperature remained between 25 ℃-30 ℃, humidity 90%-95%.Begin to refine seedling after 25 days, 30 days test statistics.
2, different root-growing agent prescriptions are to the influence of " covering Qi l number " matrimony vine plant percent
It is as shown in table 1 below to add up the number of taking root:
Table 1 for different root-growing agents to the several influence of taking root of " cover Qi l number " matrimony vine nursery stock
Figure BDA00001669226900041
Annotate: same letter representes that difference is not remarkable in the table, alphabetical different table differential different significantly (P < 0.05).
Can find out, behind the epicormic branch cuttage, the situation of taking root of different disposal carried out twice investigation statistics respectively, from table 1, can find out, behind cuttage 20d, remove contrast and do not take root basically, C1 has outside minority takes root, and other is handled and all have in a large number roots newly.Through variance analysis: the number average of taking root in 4 processing is significantly higher than contrast (CK), wherein C4 take root number at most, be 6.7 and be significantly higher than other processing; Next is that taking root of C2 and C3 counted more than 4, is significantly higher than C1; The number of taking root that C1 handles is minimum.
Statistics amount of growth and survival rate are shown in following table 2 and table 3:
Table 2 is the influences of different root-growing agents to " covering Qi l number " matrimony vine seedling growth amount
Figure BDA00001669226900042
Table 3 is that different root-growing agents are to the influence of " covering Qi l number " matrimony vine plant percent
Figure BDA00001669226900043
Annotate: same letter representes that difference is not remarkable in the table, alphabetical different table differential different significantly (P < 0.05).
From table 2, can find out in the table 3 that the root-growing agent of different formulations has facilitation in various degree to amount of growth and the survival rate of " covering Qi l number " matrimony vine nursery stock.Root length is that C1 and C3 handle and the longlyest be respectively 12.4cm and 10.8cm, is significantly higher than C2 and C4, but do not have significant difference between these two processing, and secondly be that C2 and C4 processing root length is respectively 7.6cm and 6.6cm, there is not significant difference between these two processing; The root of Ck is long the shortest.Secondly the root of C1 is C3 and C4 the most slightly for 0.18cm is significantly higher than other processing, and the rugosity of C2 is minimum not to exist significant difference with contrast.What branch length and branches and leaves number were maximum is that C3 and C4 handle, and secondly is C1 and C2.
Through the investigation discovery of above different aspect, the kind of root-growing agent and concentration are very different to the influence of the cuttage of No. 1 matrimony vine of illiteracy Qi, and methyl is handled (C1) take root quantity and survival rate height not as heteroauxin processing (C2), but root system is more sturdy.Heteroauxin handles (C2) though the number of taking root is more but root that grow is thinner, is significantly higher than independent use so two kinds of hormones mix the effect of using.But C4 is in rooting process, and wound is more, has putrefactive phenomenon to take place in the final-period management.Therefore the treatment effect of 100mg/kg methyl (NAA)+100mg/kg heteroauxin (IBA) is best.
Two, the slotting fringe of different rugosity is to the influence of " covering Qi No. 1 " matrimony vine plant percent
1, cuttage
1), cutting bed is prepared
Identical with above-mentioned one.
2), insert the preparation of fringe
Other with one 2) identical, the semi-lignified that different is chooses different rugosity covers No. 1 spray of Qi and studies.Three processing are established in test, 1. insert fringe rugosity 0.1-0.2cm; 2. insert fringe rugosity 0.2cm-0.4cm; 3. insert fringe rugosity 0.4-0.6cm.
Each handles investigation 100 strains, repeats 3 times, and each handles 300 strains altogether.
3), cuttage pre-treatment
Identical with above-mentioned one.
4) cuttage
With above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 200mg/kg NAA+200mg/kg IBA preparation.
5) seedbed management
After cuttage was intact, temperature remained between 25 ℃-30 ℃, humidity 90%-95%.Begin to refine seedling after 25 days, 30 days test statistics.
2, the slotting fringe of different rugosity is to the influence of " covering Qi l number " matrimony vine plant percent
Add up the influence of the survival rate of slotting fringe under identical management condition of different rugosity, the result is as shown in table 4.
Table 4 is the influence of the slotting fringe of different rugosity to " covering Qi l number " matrimony vine plant percent
Figure BDA00001669226900051
Annotate: same letter representes that difference is not remarkable in the table, alphabetical different table differential different significantly (P < 0.05).
From table 4, can find out: 3 kinds of differences are inserted between the plant percent of fringe rugosity all difference significantly (P < 0.05).It is the highest to insert take root quantity and survival rate of the nursery stock of fringe rugosity between 0.2-0.4cm, and respectively up to 6.3 and 88%, seedling growth is also more vigorous.Inserting the fringe rugosity is 4.9 at the nursery stock of the 0.1-0.2cm number of taking root, survival rate 60%, but to grow in the back that becomes to live generally more weak, is not easy to grow up to seedlings then.It is minimum at the plant percent of 0.4-0.6cm to insert the fringe rugosity, and survival rate has only 23%, but the nursery stock growing way that becomes to live is very prosperous.The low reason of slotting fringe survival rate that branch is thicker possibly be: what branch was thicker is water sprout mostly; Marrow is empty, and the cuttage rear lower is difficult for producing callus, perishable, so survival rate is on the low side; It is prosperous to form nursery stock growing way alive, and main cause is big, the easy accumulation nutrition of the thick blade of branch.So the slotting fringe of spray work that preferably choosing more medium (0.2-0.4cm) is transplanted a cutting in choosing should not select thick or meticulous branch.
Three, the slotting fringe of differing maturity is to the influence of " covering Qi l number " matrimony vine plant percent
1, cuttage
1), cutting bed is prepared
Identical with above-mentioned one.
2), insert the preparation of fringe
Other with one 2) identical, choose the slotting fringe of rugosity 0.3cm, and establish 4 processing again: 1. insert the fringe maturity and be lignification not; 2. inserting the fringe maturity is semi-lignified; 3. inserting the fringe maturity is lignification.Each handles investigation 100 strains, repeats 3 times, and each handles 300 strains altogether.
3), cuttage pre-treatment
Identical with above-mentioned one.
4) cuttage
With above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 200mg/kg NAA+200mg/kg IBA preparation.
5) seedbed management
After cuttage was intact, temperature remained between 25 ℃-30 ℃, humidity 90%-95%.Begin to refine seedling after 25 days, 30 days test statistics.
2, the slotting fringe of differing maturity is to the influence of " covering Qi l number " matrimony vine plant percent
The statistics survival rate is as shown in table 5,
The slotting fringe of table 5 differing maturity is to the influence of " covering Qi l number " matrimony vine plant percent
Figure BDA00001669226900061
Figure BDA00001669226900071
Annotate: same letter representes that difference is not remarkable in the table, alphabetical different table differential different significantly (P < 0.05).The tender not lignification of every branch taper, middle part semi-lignified, base portion be lignification.
Can be found out by table 5: the degree of lignification height is also very big to the influence of survival rate.There is significant difference (P < 0.05) between the plant percent of three kinds of different degrees of lignification.Not lignified branch is tender excessively, and easily dehydration is wilted, and root takes place to rot easily, and the quantity of taking root seldom has only 3.1, and survival rate is also very low has only 22%, though become seedling growth alive also very a little less than.Lignified nursery stock because the too high branches and leaves of degree of lignification are ripe, is fallen leaves again easily.Influence is taken root and is germinateed, and germination is also than other evening.The number of taking root is 3.7, and survival rate is 56%.The semi-lignified branch branches and leaves are not easy to come off, and take root number and survival rate are high, reach 4.8 and 86% respectively.Accumulated a large amount of nutrition in the branch, the seedling growth that becomes to live is very prosperous.So when selecting branch, should select the slotting fringe effect of the slotting work of the skill row culture of semi-lignified best as far as possible.
Four, different blade quantities are to the influence of " covering Qi l number " matrimony vine plant percent
1, cuttage
1), cutting bed is prepared
Identical with above-mentioned one.
2), insert the preparation of fringe
Other with one 2) identical, choose the semi-wooden scion of rugosity 0.3cm, and establish 5 processing again, 1. transplant a cutting and do not stay blade (0); 2. stay the 1-2 sheet on transplanting a cutting; 3. stay the 3-4 sheet on transplanting a cutting; 4. stay the 5-6 sheet on transplanting a cutting; 5. stay the 6-10 sheet on transplanting a cutting.Each handles investigation 100 strains, repeats 3 times, and each handles 300 strains altogether.
3), cuttage pre-treatment
Identical with above-mentioned one.
4) cuttage
With above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 200mg/kg NAA+200mg/kg IBA preparation.
5) seedbed management
After cuttage was intact, temperature remained between 25 ℃-30 ℃, humidity 90%-95%.Begin to refine seedling after 25 days, 30 days test statistics.
2, detect of the influence of different blade quantities to " covering Qi l number " matrimony vine plant percent
Through inserting the experimental study of leaving and taking different blade quantities on the fringe, add up survival rate, the result is as shown in table 6,
Table 6 is the influences of different blade quantities to " covering Qi l number " matrimony vine plant percent
Figure BDA00001669226900081
From table 6, can find do not have the slotting fringe of blade can not carry out photosynthesis, cause nutrition deficient and cause being difficult to take root, and survival rate be very low.And stay leaf too much can cause strong transpiration, and make and insert that moisture distributes in a large number in the fringe, disequilibrium, too big perishable the coming off of blade causes ground, garden germ to breed in a large number, causes disease.Through variance analysis, stay the cuttage rooting amount (4.5/fringe) and the survival rate (82%) of 3~4 leaves the highest, be significantly higher than other.Next is 5~6 leaves (72%), does not stay the slotting fringe of leaf minimum.Therefore, the blade of when cuttage, inserting fringe keeps 3~4 is advisable, but also can insert little the staying of the thick blade of fringe according to the size adjustment of rugosity of inserting fringe and blade more, inserts big then will the staying less of fringe spire sheet.
Five, the seedling cultivation greenhouse temperature is to the influence of " covering Qi l number " matrimony vine plant percent
1, cuttage
1), cutting bed is prepared
Identical with above-mentioned one.
2), insert the preparation of fringe
Other with one 2) identical, choose the slotting fringe of the semi-lignified of rugosity 0.3cm, and stay 3 blades on transplanting a cutting.Each handles investigation 100 strains, repeats 3 times, and each handles 300 strains altogether.
3), cuttage pre-treatment
Identical with above-mentioned one.
4) cuttage
With above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 200mg/kg NAA+200mg/kg IBA preparation.
5) seedbed management
After cuttage was intact, establish 3 processing: 1. temperature was made as 35 ℃~30 ℃; 2. temperature is made as 30 ℃~25 ℃; 3. temperature is made as 25 ℃~20 ℃.Each handles 100 strains of investigation nursery stock, repeats 3 times, and each handles 300 strains altogether.
Humidity is controlled at 90%-95%.Begin to refine seedling after 25 days, 30 days test statistics.
2, the seedling cultivation greenhouse temperature is to the influence of " covering Qi l number " matrimony vine plant percent
The statistics survival rate, the result is as shown in table 7,
Table 7 seedling cultivation greenhouse temperature is to the influence of " covering Qi l number " matrimony vine plant percent
Figure BDA00001669226900082
Figure BDA00001669226900091
Annotate: same letter representes that difference is not remarkable in the table, alphabetical different table differential different significantly (P < 0.05)
Temperature in the seedling cultivation greenhouse has very big relation with the survival rate of " covering Qi l number " matrimony vine spray nursery stock.The easy dehydration of the too high nursery stock of temperature is wilted, and root is difficult for taking root, and the seedlings root growth is slow, and plant percent is low, and unfavorable Cheng Miao is a little less than the seedling growth that becomes to live.Temperature is low excessively, is unfavorable for cuttage rooting, poor growth, and nursery stock is small and weak, is prone to take place butt rot.Through experimental study, temperature survival rate between 35~30 ℃ is 58%, and the quantity of taking root is 3.9/ fringe, and seedling growth is vigorous.The survival rate of temperature between 20~25 ℃ is 62%, and the number of taking root is a 2.4/fringe, and seedling growth is stronger.Temperature survival rate between 30~25 ℃ is the highest, reaches 89% seedling growth vigorous (seeing table 14).The plant percent of 3 kinds of Temperature Treatment and the significant difference between the number of taking root (P 0.05).So the temperature in greenhouse should preferably be controlled at about 30 ℃ when carrying out " covering Qi l number " matrimony vine epicormic branch cuttage, is about 30 ℃-25 ℃, maximum temperature can not surpass 35 ℃, and minimum temperature can not be lower than 20 ℃.
Six, seedling cultivation greenhouse humidity is to the influence of " covering Qi l number " matrimony vine plant percent
1, cuttage
1), cutting bed is prepared
Identical with above-mentioned one.
2), insert the preparation of fringe
Other with one 2) identical, choose the semi-wooden scion of rugosity 0.3cm, and stay 3 blades on transplanting a cutting.Each handles investigation 100 strains, repeats 3 times, and each handles 300 strains altogether.
3), cuttage pre-treatment
Identical with above-mentioned one.
4) cuttage
With above-mentioned 2) the slotting fringe base portion for preparing inserts in the sand in seedbed after being stained with the root-growing agent pasty liquid of a little 200mg/kg NAA+200mg/kg IBA preparation.
5) seedbed management
After cuttage was intact, temperature was 25 ℃-30 ℃, and establish 4 processing then: 1. humidity is made as 95%~90%; 2. humidity is made as 90~85%; 3. humidity is made as 85~80%; 4. humidity is made as 80~75%.Each handles 100 strains of investigation nursery stock, repeats 3 times, and each handles 300 strains altogether.
Begin to refine seedling after 25 days, 30 days test statistics.
2, detect of the influence of seedling cultivation greenhouse humidity to " covering Qi l number " matrimony vine plant percent
Humidity in the seedling cultivation greenhouse is to " covering Qi l number " matrimony vine spray direct relation of having grown seedlings, and humidity is too high, can cause bad root phenomenon, influences plant percent; Humidity is low excessively, is prone to cause the dehydration of spray tender leaf, causes the branch wilting, unfavorable Cheng Miao.Carry out the experimental study of 4 different humidity bench, result of study is as shown in table 8.
Table 8 is the influence of seedling cultivation greenhouse humidity to " covering Qi l number " matrimony vine plant percent
Figure BDA00001669226900101
Annotate: same letter representes that difference is not remarkable in the table, alphabetical different table differential different significantly (P < 0.05).
Show: taking root of matrimony vine nursery stock counted and the survival rate reduction along with the decline of seedling cultivation greenhouse humidity " is covered Qi l number " />.Through variance analysis (seeing table 8), the nursery stock of 4 different humidity sections take root quantity and survival rate significant difference (P < 0.05).Chamber humidity plant percent between 95%~90% is the highest, can reach 90%, and the number of taking root is a 5.2fringe, and seedling growth is prosperous.Chamber humidity plant percent between 90%~85% is 73%, and the number of taking root is a 4.8/fringe, and seedling growth is prosperous.Humidity plant percent between 85%~80% is 46%, and the number of taking root is a 4.1/fringe, seedling growth a little less than.Humidity only has 32% in that 80% following plant percent is minimum, and the number also minimum (3.2/fringe) of taking root.Therefore, the optimum humidity in greenhouse must be controlled at about 90~95% when carrying out " covering Qi l number " matrimony vine epicormic branch cutting, and minimum humidity can not be lower than 80%.
Above-mentioned experimental result is done following summary:
1) naa and two kinds of root-growing agents of earthworm diindyl butyric acid can both obviously improve amount of growth and the survival rate of the nursery stock of " covering Qi l number " matrimony vine epicormic branch cuttage; The plant percent that wherein uses 100mg/kgNAA to handle be 64% contrast exceed 1 times, the plant percent that 100mg/kgIBA handles be 75% contrast high by 43%.The plant percent that 100mg/kgNAA+100mg/kgIBA handles is 91%, and the plant percent that 200mg/kgNAA+200mg/kgIBA handles is 84%, so the effect of naa and two kinds of root-growing agents mixing of earthworm diindyl butyric acid use is better than independent result of use.The result of use of root-growing agent prescription is in proper order: 100mg/kgNAA+100mg/kgIBA>200mg/kgNAA+200mg/kgIBA>100mg/kgIBA>100mg/kgNAA.
2) find to insert the very big influence that all has alive that becomes of the number of blade, rugosity and the degree of lignification of fringe reservation through testing to " covering Qi l number " matrimony vine nursery stock.Difference is inserted the test of fringe rugosity and is found, the slotting effect of fringe rugosity between 0.2~0.4cm is best, and the survival rate and the quantity maximum of taking root are respectively 88% and 6.3; The result of the test of differing maturity is: the effect of semi-lignified degree is best, and survival rate is respectively 86% and 4.8 with the quantity of taking root; Blade is retained in 3~4 survival rate and the quantity of taking root is the highest, is respectively 82% and 4.5.Therefore when " covering Qi l number " matrimony vine epicormic branch cuttage, should select to insert the fringe rugosity is the semi-wooden scion of 0.2~0.4cm, and keeps 3~4 leaves.
3) optimum temperature of seedling cultivation greenhouse is about 30 ℃ during " cover Qi l number " matrimony vine epicormic branch cuttage, and survival rate can reach 89%; Maximum temperature can not surpass 35 ℃, and minimum temperature can not be lower than 20 ℃.
4) the matrimony vine plant percent descends with the reduction of seedling cultivation greenhouse humidity " to cover Qi l number ".The humidity of seedling cultivation greenhouse preferably remains between 95%~90% during epicormic branch cuttage, and survival rate is up to 90%; Minimum humidity can not be lower than 80% (humidity is lower than 80% following survival rate only has 32%).

Claims (10)

1. a matrimony vine epicormic branch cuttage method comprises the steps:
1) branch of clip matrimony vine tree is as inserting fringe;
2) with said slotting fringe with the compositions-treated of taking root, obtain handling the back and insert fringe;
3) inserted the fringe cuttage, grow seedlings in said processing back, realize the matrimony vine epicormic branch cuttage.
2. method according to claim 1 is characterized in that:
Step 2) in, the said composition of taking root is by solute and solvent composition, and said solute is made up of 2 kinds of materials with following mass ratio: naa: indolebutyric acid is 1:1.
3. method according to claim 1 and 2 is characterized in that:
Said solvent is a water;
Said naa and the said indolebutyric acid final concentration in the said composition of taking root is 100mg/kg-200mg/kg, and said naa and the said indolebutyric acid final concentration in the said composition of taking root all is specially 200mg/kg.
4. according to arbitrary described method among the claim 1-3, it is characterized in that:
Said use is taken root compositions-treated for said slotting fringe base portion is picked the said composition of taking root.
5. according to arbitrary described method among the claim 1-4, it is characterized in that:
In the step 1), the rugosity of said slotting fringe is 0.2cm-0.4cm, and the rugosity of said slotting fringe is specially 0.3cm;
Said slotting fringe is a semi-lignified;
Lobe numbers on the said slotting fringe is 3-4, and the lobe numbers on the said slotting fringe is specially 3.
6. according to arbitrary described method among the claim 1-5, it is characterized in that:
In the step 3), said temperature of growing seedlings is 20 ℃-35 ℃; Said temperature of growing seedlings is specially 25 ℃-30 ℃;
Said humidity of growing seedlings is 85%-95%; Said humidity of growing seedlings is specially 90%-95%.
7. composition of taking root, by solute and solvent composition, said solute is made up of 2 kinds of materials with following mass ratio: naa: indolebutyric acid is 1:1.
8. composition according to claim 7 is characterized in that:
Said solvent is a water.
9. composition according to claim 8 is characterized in that:
Said naa and the said indolebutyric acid final concentration in the said composition of taking root is 100mg/kg-200mg/kg, and said naa and the said indolebutyric acid final concentration in the said composition of taking root all is specially 200mg/kg.
Among the claim 7-9 arbitrary described composition at the plant epicormic branch cuttage or promote the application during spray is taken root in the plant cuttage; Said plant is specially matrimony vine.
CN2012101598526A 2012-05-22 2012-05-22 Twig cutting method of Lycium Chinense Mill. of variety Mengqi No.1 Pending CN102696370A (en)

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CN104082014A (en) * 2014-07-19 2014-10-08 白银市农业技术服务中心 Medlar seedling growing method
CN104737754A (en) * 2015-03-26 2015-07-01 广西壮族自治区农业科学院园艺研究所 Method for improving atemoya cutting seedling surviving rate
CN106305274A (en) * 2016-08-20 2017-01-11 阿拉善盟林木种苗站 Shoot cutting method for lyciumruthenicum murayr
CN107114179A (en) * 2017-04-18 2017-09-01 酒泉市祁连花青有机生态农产品开发有限公司 A kind of golden matrimony vine planting technology in salt-soda soil
CN108834896A (en) * 2018-07-17 2018-11-20 宁夏正杞红枸杞产业发展有限公司 A kind of fructus lycii implantation methods based on peaceful Qi 6 and peaceful Qi 8
CN115380751A (en) * 2022-05-10 2022-11-25 深圳市芭田生态工程股份有限公司 Cultivation method of Chinese wolfberry and selenium-rich Chinese wolfberry

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082014A (en) * 2014-07-19 2014-10-08 白银市农业技术服务中心 Medlar seedling growing method
CN104737754A (en) * 2015-03-26 2015-07-01 广西壮族自治区农业科学院园艺研究所 Method for improving atemoya cutting seedling surviving rate
CN106305274A (en) * 2016-08-20 2017-01-11 阿拉善盟林木种苗站 Shoot cutting method for lyciumruthenicum murayr
CN107114179A (en) * 2017-04-18 2017-09-01 酒泉市祁连花青有机生态农产品开发有限公司 A kind of golden matrimony vine planting technology in salt-soda soil
CN108834896A (en) * 2018-07-17 2018-11-20 宁夏正杞红枸杞产业发展有限公司 A kind of fructus lycii implantation methods based on peaceful Qi 6 and peaceful Qi 8
CN115380751A (en) * 2022-05-10 2022-11-25 深圳市芭田生态工程股份有限公司 Cultivation method of Chinese wolfberry and selenium-rich Chinese wolfberry
CN115380751B (en) * 2022-05-10 2023-07-25 深圳市芭田生态工程股份有限公司 Cultivation method of wolfberry and selenium-enriched wolfberry

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